JP6436799B2 - Cell culture management device, cell culture terminal device, and cell culture system - Google Patents

Description

  The present invention relates to an apparatus for cell culture.

The configuration of a conventional cell culture apparatus is as follows.
That is, a cell culture device is disclosed that receives cells to be cultured from a clean space formed in a cell manipulation chamber and forms a culture space in which the cells are cultured (for example, Patent Document 1).

JP 2011-160672 A

The problem with the conventional example is that many man-made operations occur in the process of cell culture, and there are multiple types of cells to be cultured. Needed to be uniform. However, it has been difficult to perform these processes artificially.
Therefore, an object of the present invention is to simplify the cell culture process.

  And in order to achieve this object, the present invention is a cell culture management device connected to a cell culture terminal for culturing cells, an identification information generating unit for generating identification information of a container into which cells are placed, and a plurality of containers Database information storing the identification information of these containers, cell concentration and liquid volume information of these containers, identification information of a plurality of containers stored in the database section, and cell concentration and liquid volume information of a plurality of containers And a control unit that issues a work instruction to the cell culture terminal.

  This achieves the intended purpose.

  According to the present invention, the cell culture process can be simplified.

The control block diagram of the cell culture system which concerns on one Embodiment of this invention. The control block diagram of a cell culture management apparatus. The control block diagram of a server part and a unit part. The control block diagram of a cell raising part. The flowchart of the process. The flowchart of the process. The flowchart of communication of a cell raising part and a server part. The flowchart of communication of a cell raising part and a server part. The flowchart of communication of a cell raising part and a server part. The control block diagram of a density | concentration adjustment sowing part. The flowchart of the process. The control block diagram of a cell culture part. The flowchart of the process. The top view of a centrifuge. The perspective view of a cell loosening machine.

DESCRIPTION OF EXEMPLARY EMBODIMENTS Hereinafter, an embodiment of a cell culture terminal, a cell culture management device, and a cell culture system including these according to an embodiment of the present invention will be described with reference to the accompanying drawings.
As shown in FIG. 1, the configuration of the entire cell culture system is divided into a client unit 1, a server unit 2, and a unit unit 3.

An apparatus including the client unit 1 and the server unit is a cell culture management device, and a terminal group including the unit unit 3 is referred to as a cell culture terminal device.
The client unit 1 is made up of three blocks. The manager performs scheduling management and sample acceptance management.
FIG. 2 shows a block diagram inside the client unit 1 and the server unit 2.

The first is the process management unit 4 which displays the cell culture process for the specimen performed in the unit unit 3 to the administrator.
The second is the barcode printing unit 5, that is, the identification information generating unit.
This prints in advance a barcode attached to a container in which a specimen is stored, a barcode attached to a container containing a reagent, and a barcode attached to an installation place of the containers. The barcode information to be printed is registered in advance in the database unit 6 provided in the server unit 2.

The identification information of the container into which the cells generated by the identification information generation unit and the identification information of the installation unit in which the container is installed are associated with each step of culturing cells in the process management unit 4.
The identification information is information associated with which cell is placed in which container and in which installation place in each step of culturing the cells by the process management unit 4 before cell culture. Is set.

The third is the sample entry unit 7. In order to confirm the sample delivered from the supplier, the barcode information affixed to the container in which the sample is placed is read and this barcode information is confirmed. Check if the sample was delivered correctly.
The server unit 2 manages processes while managing data and exchanging information with the unit unit, and is composed of two blocks.

The first is the database unit 6, which contains bar code information for identifying the specimen.
The second unit is a unit control unit 8 that manages processes while exchanging with the unit unit 3.
FIG. 3 shows a block diagram of the inside of the unit unit 3.

The unit part 3 is composed of three blocks.
The first is a cell raising part 9 which enters a small container, loosens the delivered sample cells, transfers them to a large container as a cell group of an appropriate concentration, and selects a cell group as a specimen for cell culture. It is a block which performs the process to prepare.
The second is the concentration-adjusted seeding unit 10, where the sample cells in the large container are added with a medium to obtain a predetermined concentration, and the sample cells are divided into 16 plates and 3 block well plates. It is a block that performs a process.

The third is the cell culture unit 11 for culturing the sample cells in the well plate, and then adding a predetermined reagent to the cultured sample cells according to a predetermined schedule, and then performing color determination, It is a block which performs the process of observing the culture state of a sample cell.
Now, the process of cell culture will be described more specifically.
As an example, a process for monitoring the reaction to three types of reagents for patient P will be described as an example.

<Configuration of cell raising portion 9>
First, a control block diagram of the cell raising unit 9 is shown in FIG.
The cell raising unit 9 includes an input unit 17, a first cell refill unit 18, a centrifuge unit 19, a medium adjustment unit 20, a cell loosening unit 21, a cell agitation unit 22, and a second cell refill unit 23. , And a specimen cell extraction unit 24, a cell concentration measurement unit 25, a control unit 26, and an operation unit 27.

The input unit 17 is supplied with a sample container and a reagent.
The first cell refilling unit 18 transfers the sample cells placed in the container installed in the loading unit 17 to another middle container, and measures the liquid volume and cell concentration of the cells in the middle container.
The centrifuge 19 separates the cells that have entered the inner container into cells and culture supernatant using a centrifuge.

The medium adjusting unit 20 adjusts the medium by removing the medium supernatant, adding a new medium, or the like.
The cell loosening unit 21 performs a process of loosening cells that have solidified in a part of the container (the bottom or the like) by the process by the centrifugal separator 19 by applying vibration or the like to the container.
The cell agitation unit 22 agitates the sample cells.

The second cell refill unit 23 adjusts the medium of the stirred sample cells and refills the large container.
The sample cell take-out unit 24 takes out the sample cells refilled in a large container.
The cell concentration measuring unit 25 measures the number of sample cells.
The control unit 26 controls the sample while communicating with the server unit according to a predetermined process.
The operation unit 27 and a touch panel type operation panel are used for user operation input.

<Traceability of cell raising part 9>
Next, securing traceability of the cell raising part will be described.
First, as an example of this embodiment, the reaction of three types of reagents is examined for a patient P. As the sample cells, a total of 12 samples are put into a small container (2 ml) containing the sample cells, 4 for each reagent.
And as a reagent, three types of reagent, Reagent A, Reagent B, and Reagent C, are delivered in a container.

Then, a container containing a medium for culturing the specimen cells is delivered.
About barcodes affixed to containers containing specimens, barcodes affixed to containers containing reagents, barcodes affixed to containers containing culture media, and barcodes affixed to installation locations of these containers, The bar code information is input and printed by the administrator in the bar code printing unit 5 of the client unit 1, and the bar code information is stored in the database unit 6 of the server unit 2. .

The barcode information is associated with the patient information in the database unit 6 and also associated with the process management information input from the process management unit 4 of the client unit 1 by the administrator.
First, barcode information of 12 small containers containing specimens delivered from a vendor, barcode information of 3 containers containing 3 reagents (reagent A, reagent B, reagent C), and medium The bar code information of the container in which is put is read by the operator at the handy terminal. Then, the read information is transferred to the unit control unit 8 of the server unit 2 through the control unit 26 of the cell raising unit 9.

In the unit control unit 8, the read barcode information and the barcode information stored in the database unit 6 of the server unit 2 are collated, and a specimen, a reagent, and a medium related to cell culture of a desired patient are delivered. Check whether there is any.
The handy terminal is a terminal with a barcode reading function, and also includes a display unit.

  When the barcode information of the container is read at this handy terminal, the result of whether or not the barcode is appropriate is displayed on the display unit. When the barcode is appropriate, the display unit displays where the container should be placed in the charging unit.

<Process of cell raising part 9>
Next, the process will be specifically described with reference to FIG.
The process is executed by the server unit 2, the client unit 1, the unit unit 3, the handy terminal, and the operator.
First, the operator operates the touch panel as the operation unit 27 provided in the cell raising unit 9 to set the mode of the cell raising unit 9 to the replenishment mode.
The replenishment mode is a mode for confirming whether there is an error in the input specimen and reagent before the start of the process.

  Next, the worker installs the delivered small container in a predetermined input unit 17 displayed on the handy terminal. The barcode information read by the barcode reader provided at the installation location of the input unit 17 is referred to the barcode information in the database unit 6 of the server unit 2 via the control unit 26. Then, it is confirmed whether or not the sample container is placed at the correct installation position (S1).

If the confirmed content is correct, the control part 26 of the cell raising part 9 will be in the state which can transfer to an operation mode. Then, the operator operates the touch panel of the operation unit 27 to set the operation mode.
Next, the sample cells contained in six 2 ml small containers installed in the input unit 17 are moved to the first cell refill unit 18 and refilled into six 15 ml medium containers, respectively. When refilling, the liquid amount and cell concentration of each of the six inner containers are measured. Then, the control unit 26 of the cell raising unit 9 transmits the barcode information attached to the refill destination container, the liquid amount and the cell concentration of the container to the unit control unit 8 of the server unit 2. The unit control unit 8 associates the barcode information of the refill destination, the liquid amount of the container, and the cell concentration with the sample information and stores them in the database unit 6 (S2).

Then, the sample cells that have entered the inner container move to the centrifuge 19 and are arranged in a balanced manner with respect to the installation base of the centrifuge 19. This arrangement is instructed from the unit control unit 8 of the server unit 2 to the control unit 26 of the cell raising unit 9. In the centrifuge 19, the sample cells are separated into cells and culture supernatant in the container by centrifugation (S3).
The rotation speed and time in the centrifugal separator 19 are controlled by the liquid volume and cell concentration of the container.

Specifically, the higher the cell concentration, the higher the rotation speed and the longer the time for centrifugation.
In the cell culture management device, the rotational speed and time in the centrifuge 19 of the container are calculated based on the identification information of the container in which the cells are placed and the information on the liquid amount and the cell concentration of the container.
Such a configuration makes it possible to perform appropriate centrifugation with respect to the cell concentration, so that the separation time can be optimized, the time efficiency can be improved, and the cells in the container can be cultured with the cells. It becomes possible to separate properly.

As a result, the cell culture process is simplified.
Then, after the unnecessary culture supernatant is removed in the medium adjustment unit 20 (S4), the cells are loosened in the cell loosening unit 21 (S5), and a new medium is added in the medium adjustment unit 20 (S6). ).
FIG. 15 shows the configuration of the cell loosening machine in the cell loosening unit 21.

The cell loosening machine 41 holds the container 42 in a fixed state, and strikes the container 42 with a shock by striking the container 42 while reciprocating horizontally with respect to the lower end of the container 42 fixed and held by the holding body 43. And a hitting portion 44 that loosens the cells in the container.
The magnitude of the impact force can be controlled by setting the speed of moving the hitting portion 44 horizontally and its period. The greater the horizontal movement speed, the greater the impact force.

In addition, the operation time of the hitting unit 44 can be set, and the longer the total operation time, the greater the degree of cell loosening.
In the cell culture management device, the magnitude of the impact force applied to the container and the operating time for loosening are calculated based on the identification information of the container in which the cells are placed and the information on the liquid volume and cell concentration of the container.

The magnitude and operating time of the impact force of the cell loosening machine 41 are controlled by the amount of liquid in the container and the cell concentration.
Specifically, the higher the cell concentration, the larger the impact force and the longer the operation time.
The unit control unit 8 that has received the information is configured to control the cell loosening device by applying an impact force for a predetermined time to the container in which the cells are placed.

With such a configuration, it becomes possible to perform appropriate cell loosening with respect to the cell concentration, so that it is possible to optimize operation time, improve time efficiency, and loosen cells appropriately. Become.
As a result, the cell culture process is simplified.
Then, after being stirred in the cell agitation unit 22 (S7), after passing through the centrifugal separation unit 19 again (S8), unnecessary culture supernatant is removed in the medium adjustment unit 20 (S9). And after loosening a cell in the cell loosening part 21 (S10), a culture medium is newly added in the culture medium adjustment part 20 (S11).

  Then, in the second cell refilling unit 23, it is divided into three 50 ml large containers and three 2 ml small containers. At this time, the control unit 26 of the cell raising unit 9 transmits the barcode information attached to the refill destination container to the unit control unit 8 of the server unit 2. The unit control unit 8 associates the refill destination barcode information with the sample information and stores it in the database unit 6 (S12).

  Then, while measuring the amount of liquid in a 50 ml large container, the cell concentration measuring unit 25 measures the number of specimen cells in the 50 ml and the number of specimen cells in a 2 ml container corresponding to each. Then, the cell concentration is converted (S13). Then, the information on the amount of the sample contained in the 50 ml large container and the cell concentration thereof is sent from the control unit 26 to the database unit 6 of the server unit 2. In the database unit 6, the liquid volume and the cell concentration are stored in association with the barcode information attached to the large container.

As a result of the cell raising unit 9, the sample cell contained in a large 50 ml container is extracted from the sample cell extracting unit 24 (S14).
As described above, in the embodiment of the present invention, the confirmation of the sample and the trace recording of the sample are performed while the unit unit 3 and the server unit 2 confirm the sample in cooperation with each step. This will be described in more detail.

FIG. 6 shows communication between the cell raising part 9 and the server part 2 in the step (S1) of checking the input member in FIG.
First, the specimen, the reagent, and the medium that are input to the input unit 17 of the cell raising unit 9 are sent from the control unit 26 of the cell raising unit 9 to the server unit 2 as barcode information attached to the container in which they are stored. To the unit controller 8 (S15).

The transmitted barcode information is inquired to the database unit 6 via the unit control unit 8 of the server unit 2 (S19). Then, it is confirmed whether the input specimen, the reagent, and the medium are correct. The result is transmitted from the unit control unit 8 to the control unit 26 of the cell raising unit 9 (S20).
Next, the control unit 26 of the cell raising unit 9 inquires as to whether or not the specimen, the reagent, and the medium have been placed at the correct installation position of the input unit 17 (S17). Specifically, for the specimen, the reagent, and the culture medium, the barcode information read by the barcode reader provided at the installation location of the container in which they are stored is sent from the control unit 26 of the cell raising unit 9 to the server unit. 2 is sent to the unit control unit 8 (S17). Then, it is confirmed whether or not the input specimen, the reagent, and the medium are installed at the correct positions (S21). Then, the result is transmitted from the unit control unit 8 to the control unit 26 of the cell raising unit 9 (S22).

FIG. 7 shows communication between the cell raising part 9 and the server part 2 in the container cell refilling step (S2) of FIG.
Information on the refill destination container of the specimen is sent from the control unit 26 of the cell raising unit 9 to the unit control unit 8 of the server unit 2 (S23). The information on the container to which the sample is refilled is linked and stored as sample information in the database unit 6 of the server unit 2 (S24).

FIG. 8 shows communication between the cell raising part 9 and the server part 2 in the step (S3, S8) of centrifuging the sample cell of FIG.
FIG. 20 shows a top view of the rotating part of the centrifuge.
Four buckets 59 a, 59 b, 59 c, 59 d are provided in the rotating part of the centrifuge at positions shifted by a rotation angle of 90 degrees with the rotation center 58 as the center as container mounting bases.

Each bucket is provided with 2 rows × 4 holders 60 for arranging containers containing cells.
When the rotational speed is increased in a state where the centrifuge is not balanced, a large vibration is generated, and there is a danger such as jumping out of contents. Therefore, it is desirable that the difference in the weight of the object between the buckets facing the rotation center is small.

First, the unit controller 8 of the server unit 2 calculates how to arrange the cell containers with respect to the holder of the bucket 59 of the centrifuge. The database unit 6 of the server unit 2 contains information on the liquid volume of the cell container, and the calculation is performed so that the liquid volume distribution between the buckets facing each other is equal to the rotation center 58.
More specifically, the containers are sequentially arranged in descending order of the liquid volume of the cell containers to be introduced at the holder positions (for example, 60a and 60b) that are point-symmetric with respect to the rotation center 58 between the opposing buckets. Calculate how to arrange them. When the number of cell containers to be introduced is an odd number, calculation is performed so that dummy containers having a weight corresponding to the weight of one cell container are arranged on the other side. And after calculating how to arrange all the containers, the unit control part 8 of the server part 2 sends the information on how to arrange to the control part 26 of the cell raising part 9 (S26). Based on the information, the control unit 26 of the cell raising unit 9 arranges the cell containers on the bucket of the centrifuge (S25). As a result, the centrifuge can operate the centrifuge in a balanced state.

In the above, the order in which the cell containers are arranged is the order in which the liquid amount is large. However, the order is not limited to this, and an arrangement method using a general mathematical search algorithm may be used.
In addition, the means for arranging the containers in the bucket 59 of the centrifuge is performed by a transport unit having a robot arm. This transport unit is controlled by the control unit 26 of the cell raising unit 9.

The control program for the cell raising part 9 is programmed according to the above steps.
FIG. 9 shows communication between the cell raising unit 9 and the server unit 2 in the step (S13) of measuring the liquid amount and cell concentration of the specimen cell in FIG.
Information on the liquid volume and cell concentration of the specimen is sent from the control unit 26 of the cell raising unit 9 to the unit control unit 8 of the server unit 2 (S27). The information on the container to which the sample is refilled is linked and stored as sample information in the database unit 6 of the server unit 2 (S28).

  As described above, the server unit 2 and the unit unit 3 associate the sample information as sample information while updating in which container the liquid amount and the cell concentration are stored for each process. The information is stored in the database unit 6. By doing so, it is possible to easily ensure the traceability of the specimen, so that the cell culture of the specimen can be performed correctly.

  Furthermore, since the identification information of the container in which the cells linked by the process management unit are placed and the identification information of the installation unit in which the container is installed are compared and determined and collated, there is an effect of preventing human error. .

<Configuration of Concentration Adjustment Sowing Unit 10>
Next, a control block diagram of the concentration adjusting seeding unit 10 is shown in FIG.
The concentration adjusting seeding unit 10 removes the culture supernatant from the sample container, the input unit 28 for supplying the reagent, and the sample cells contained in the large container installed in the input unit 28, or adds the medium. A concentration adjusting unit 29 for making a predetermined concentration, a cell seeding unit 30 for seeding cells in a large container on a 16-block * 3 well plate according to instructions of the server unit 2, and a well plate on which cells are seeded An extraction unit 31 for extraction setting, a control unit 32 for controlling the sample while communicating with the server unit 2 according to a predetermined process, and an operation unit 33 operated from a touch panel are configured.

And, in the cell culture management device that is the host system, based on the identification information of the container in which the cells stored in the database unit 6 are put, and the cell concentration and liquid volume information of this container, A cell concentration adjustment instruction for adjusting the cell concentration of the container and a cell liquid volume adjustment instruction for adjusting the liquid volume of the container are sent to the control unit 32.
The control unit 32 controls the concentration adjusting unit 29 so that the container in which the cells are placed has a predetermined concentration.
Specifically, if the cell concentration is too low, the medium supernatant is removed. On the other hand, if the cell concentration is too high, the medium is added to obtain a predetermined concentration.

<Traceability of concentration-controlled sowing unit 10>
Next, the traceability ensuring of the concentration adjustment seeding part will be described.
First, three large containers corresponding to each of the three types of reagents are introduced from the cell raising portion 9. In these three large containers, the cell concentration and the liquid amount measured by the cell raising unit 9 are linked in the database unit 6 of the server unit 2.

For the barcodes affixed to the large containers in which the specimens are stored and the barcodes affixed to the installation locations of these containers, the barcode information is input by the administrator in the barcode printing unit 5 of the client unit 1. Things are printed. The bar code information is stored in the database unit 6 of the server unit 2.
The barcode information is associated with the patient information in the database unit 6 and also associated with the process management information input from the process management unit 4 of the client unit 1 by the administrator.

First, the bar code information of the three large containers is read by the operator at the handy terminal, and the information is transferred to the unit control unit 8 of the server unit 2 through the control unit 32 of the concentration adjustment seeding unit 10. .
In the unit control unit 8, the read barcode information is collated with the barcode information stored in the database unit 6 of the server unit 2, and whether or not a specimen related to cell culture of a desired patient is input. Confirm.

The handy terminal is a terminal with a barcode reading function, and also includes a display unit.
When reading the barcode information of the container with this handy terminal, the result of whether or not the barcode is appropriate is displayed on the display unit. If the bar code is appropriate, the display unit displays where the container should be placed in the input unit 28.

  In the cell seeding unit 30, the specimens in the three large containers are seeded in three blocks of the well plate, respectively. Each block of the well plate has 16 wells, and a specimen is seeded in a predetermined well preset in the database unit 6 of the server unit 2. This well plate also has barcode information, and is associated with the barcode information of the three large containers in the database unit 6 of the server unit 2.

<Process of concentration adjustment seeding part 10>
Next, the process will be specifically described with reference to FIG.
The process is executed by the server unit 2, the client unit 1, the unit unit 3, the handy terminal, and the operator.
First, the operator operates the touch panel as the operation unit 33 provided in the concentration adjustment seeding unit 10 to set the mode of the concentration adjustment seeding unit 10 to the replenishment mode.

The replenishment mode is a mode for confirming whether there is an error in the input specimen and reagent before the start of the process.
Next, the worker installs the delivered large container in a predetermined charging unit 28 displayed on the handy terminal. The barcode information read by the barcode reader provided at the installation location of the input unit 28 is referred to the barcode information of the database unit 6 of the server unit 2 via the control unit 32. Then, it is confirmed whether or not the sample container is placed at the correct installation position (S29).

If the confirmed content is correct, the control unit 32 of the concentration adjusting seeding unit 10 is in a state where it can shift to the operation mode. Then, the operator operates the touch panel of the operation unit 33 to set the operation mode.
Next, the specimen cells in the three 50 ml large containers installed in the input unit 28 are moved to the concentration adjusting unit 29. Here, based on the identification information of the container in which the cells are sent from the cell culture management device, the cell concentration adjustment instruction for adjusting the cell concentration of the container, and the cell liquid volume adjustment instruction for adjusting the liquid volume of the container. When the cell concentration is too low, a treatment for removing the culture supernatant is performed. On the other hand, if the cell concentration is too high, a process of adding a medium to a predetermined concentration is performed (S30).

  Next, the sample cells in the three large containers are seeded in a well plate. At this time, the control unit 32 of the concentration adjustment seeding unit 10 transmits the barcode information attached to the well plate to the unit control unit 8 of the server unit 2. The unit control unit 8 stores the barcode information of the well plate in the database unit 6 in association with the sample information. Then, the unit control unit 8 instructs the control unit 32 as to which well on the well plate is to be seeded (S31).

As a result of the concentration-adjusted seeding unit 10, the sample cells that have entered the well plate are extracted from the extraction unit 31 (S32).
The control program of the concentration adjusting seeding unit 10 is programmed according to the above-described process.
As described above, the server unit 2 and the unit unit 3 associate the sample information as sample information while updating in which container the liquid amount and the cell concentration are stored for each process. The information is stored in the database unit 6. By doing so, it is possible to easily ensure the traceability of the specimen, so that the cell culture of the specimen can be performed correctly.

<Configuration of cell culture unit 11>
Next, a control block diagram of the cell culture unit 11 is shown in FIG.
The cell culture unit 11 includes an input unit 34, a medium exchange unit 35, a reagent addition unit 36, a cell determination unit 37, a removal unit 38, a control unit 39, and an operation unit 40.
The input unit 34 inputs a well plate and a reagent.

The medium exchange unit 35 exchanges the medium of the sample cells in the well plate installed in the input unit 34 according to the number of culture days.
The reagent adding unit 36 adds a reagent.
The cell determination unit 37 determines how much the cultured cells are cultured.
The take-out unit 38 takes out and sets a well plate in which cells are cultured.

The control unit 39 controls the sample while communicating with the server unit 2 according to a predetermined process.
The operation unit 40 is a touch panel type operation panel, and a user's operation input is performed.

<Traceability of cell culture unit 11>
Next, ensuring traceability of the cell culture unit 11 will be described.
First, a well plate is introduced from the concentration adjusting seeding unit 10.
The bar code affixed to the well plate and the bar code information of the bar code affixed to the place where the well plate is installed are printed by the administrator in the bar code printing unit 5 of the client unit 1. The bar code information is stored in the database unit 6 of the server unit 2.

The barcode information is associated with the patient information in the database unit 6 and also associated with the process management information input from the process management unit 4 of the client unit 1 by the administrator.
First, the barcode information of the well plate is read by the operator at the handy terminal, and the information is transferred to the unit control unit 8 of the server unit 2 through the control unit 39 of the cell culture unit 11.

In the unit control unit 8, the read barcode information is collated with the barcode information stored in the database unit 6 of the server unit 2, and whether or not a specimen related to cell culture of a desired patient is input. Confirm.
The handy terminal is a terminal with a barcode reading function, and also includes a display unit.

When the barcode information of the container is read at this handy terminal, the result of whether or not the barcode is appropriate is displayed on the display unit. Then, on the display unit, when the bar code is appropriate, the position of the container in which the container is to be installed is displayed.
In the cell culture unit 11, there are 16 wells in the three blocks of the well plate, and the specimen is cultured in a predetermined well preset in the database unit 6 of the server unit 2. This well plate also has barcode information, and is associated with the barcode information of the three types of reagents in the database unit 6 of the server unit 2.

<Process of cell culture unit 11>
Next, the process will be specifically described with reference to FIG.
The process is executed by the server unit 2, the client unit 1, the unit unit 3, the handy terminal, and the operator.
First, the operator operates the touch panel as the operation unit 40 provided in the cell culture unit 11 to set the mode of the cell culture unit 11 to the replenishment mode.

The replenishment mode is a mode for confirming whether there is an error in the input specimen and reagent before the start of the process.
Next, the worker installs the well plate input via the incubator in a predetermined input unit 34 displayed on the handy terminal. The barcode information read by the barcode reader provided at the installation location of the input unit 34 is referred to the barcode information in the database unit 6 of the server unit 2 via the control unit 39. Then, it is confirmed whether or not the well plate is placed at the correct installation position (S33).

If the confirmed content is correct, the control unit 39 of the cell culture unit 11 is in a state where it can shift to the operation mode. Then, the operator operates the touch panel of the operation unit 40 to set the operation mode.
Next, the specimen cell that has entered the well plate installed in the input unit 34 is divided into three steps according to the number of days of cell culture (S34). If the number of cell days is less than or equal to the predetermined number, the medium is changed and cell culture is continued (S35). If it is a predetermined day, a predetermined reagent is added (S36). If the predetermined number of days is exceeded, it is determined whether or not the cells are cultured (S37).

As a result of the cell culturing unit 11, the sample cells contained in the well plate cultured with the predetermined reagent added are extracted from the extraction unit 38 (S38).
The control program of the cell culture part 11 is programmed according to said process.

  As described above, the server unit 2 and the unit unit 3 associate the sample information as sample information while updating in which container the liquid amount and the cell concentration are stored for each process. The information is stored in the database unit 6. By doing so, it is possible to easily ensure the traceability of the specimen, so that the cell culture of the specimen can be performed correctly.

In the present embodiment, the movement of the sample cell between the units has been described as an artificial operation by the operator. However, this operation may be performed by a transfer robot.
The movement and operation of the specimen cell in the unit are performed by a robot.

  The present invention exerts an effect that the process of cell culture can be simplified, and thus can be widely applied to a cell culture apparatus that performs cell culture.

DESCRIPTION OF SYMBOLS 1 Client part 2 Server part 3 Unit part 4 Process management part 5 Barcode printing part 6 Database part 7 Sample entry part 8 Unit control part 9 Cell raising part 10 Concentration seeding part 11 Cell culture part 17 Input part 18 1st cell refilling Unit 19 Centrifugation unit 20 Medium adjustment unit 21 Cell loosening unit 22 Cell agitation unit 23 Second cell refilling unit 24 Sample cell removal unit 25 Cell concentration measurement unit 26 Control unit 27 Operation unit 28 Input unit 29 Concentration adjustment unit 30 Cell seeding unit 31 Extraction unit 32 Control unit 33 Operation unit 34 Input unit 35 Medium exchange unit 36 Reagent addition unit 37 Cell determination unit 38 Extraction unit 39 Control unit 40 Operation unit 41 Cell loosening machine 42 Container 43 Holder 44 Tapping unit 58 Rotation center 59, 59a, 59b, 59c, 59d Bucket 60 Holder AC Medicine P Patient

Claims (8)

A cell culture management device connected to a cell culture terminal for culturing cells,
An identification information generating unit for generating identification information of a container for containing cells;
A database section for storing a plurality of identification information of the containers, cell concentration and liquid volume information of the containers,
Based on the identification information of the plurality of containers stored in the database unit, and the cell concentration and liquid volume information of the plurality of containers, a control unit that gives work instructions to the cell culture terminal;
Equipped with a,
The control unit calculates the weight of the container based on the identification information of the plurality of containers stored in the database unit and the cell concentration and liquid volume information of the plurality of containers, and a predetermined weight distribution The plurality of containers are arranged in a plurality of groups.
Cell culture management device. The control unit, based on the container identification information stored in the database unit and the cell concentration and liquid volume information of the container, the magnitude and impact of the impact force applied to the container to loosen the cells And the duration when the is granted,
The cell culture management apparatus according to claim 1. The control unit adjusts the concentration of cells in the container for cell culture processing based on the container identification information stored in the database unit and the cell concentration and liquid volume information of the container. A cell concentration adjustment instruction, a cell liquid volume adjustment instruction for adjusting the liquid volume of the container, and a work instruction to the cell culture terminal,
The cell culture management apparatus according to claim 1. A cell culture terminal device connected to the cell culture management device according to claim 1 and culturing cells according to an instruction from the cell culture management device,
A centrifuge for centrifuging a plurality of containers containing cells;
A transport unit for disposing a plurality of containers on an installation base of the centrifuge;
In accordance with the group information of the plurality of containers received from the cell culture management device, the control unit that controls the transport unit to place the plurality of containers containing cells on the centrifuge mounting table,
A cell culture terminal device. A cell culture terminal device connected to the cell culture management device according to claim 2 and culturing cells in accordance with an instruction from the cell culture management device,
A cell unraveling machine that applies an impact force for a predetermined time to a container containing cells,
Based on the identification information of the container in which the cell is placed from the cell culture management device, the magnitude of the impact force applied to the container to loosen the cell, and the duration when the impact is applied, the cell loosening machine A control unit for controlling
A cell culture terminal device. A cell culture terminal device connected to the cell culture management device according to claim 3 and culturing cells according to an instruction from the cell culture management device,
About the container containing the cells, remove the medium supernatant or add a new medium and adjust the medium to adjust the concentration of the cells,
Based on the container identification information received from the cell culture management device and the cell concentration and liquid volume information of the container, so that the cell concentration in the container in which the cells are placed becomes a predetermined concentration. A control unit for controlling the density adjustment unit;
A cell culture terminal device. It further comprises a measuring unit for measuring the identification information of the container in which the cells are put, the liquid amount of the container and the cell concentration,
The control unit notifies the cell culture management device of information measured in the measurement unit,
The cell culture terminal device according to any one of claims 4 to 6 . The cell culture management device according to any one of claims 1 to 3 ,
The cell culture terminal device according to any one of claims 4 to 7 ,
With
The cell culture terminal device is connected to the cell culture management device,
Cell culture system.

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