JP6471689B2 - Colony detector, medium information registration system, program and hygiene management system - Google Patents

Description

  The present invention relates to a food hygiene management system and the like.

  In recent years, due to the growth of fast food and other food service industries and the increase in easy processed foods, the opportunity to eat food prepared by others has increased remarkably. On the other hand, when foods such as processed foods and processed foods are mixed with dangerous substances such as poisons or microorganisms, it directly affects the human body. When handling processed foods, not only the safety of raw materials but also the production There is a need for food safety that strictly controls the hygiene of the process and covers the entire production and processing of food.

  In particular, food poisoning incidents that have occurred in various places are the starting point, and it is a proposition to further improve the safety of foods. In addition to complying with the Food Sanitation Law, “HACCP (Hazard Analysis Critical) The number of companies that introduce “Control Point) System” and “FSSC (Food Safety System Certification) 22000” is also increasing.

  As a system for detecting microorganisms mixed in such processed foods, a film-type medium is used, a specimen cultured in the film-type medium is imaged, and image processing is performed on the image of the imaged medium. In addition, detection of colonies that are a mass of bacteria generated in a specimen and counting them are known (for example, Patent Document 1 or 2). In addition, recently, while binarizing the feature amount of each pixel of an image of a medium imaged using a plurality of threshold values, two pixels having different threshold values at different threshold values are physically adjacent to each other. Is also known (for example, Patent Document 3).

JP 2011-2112013 A JP 2012-135240 A International publication WO2000-039329 pamphlet

  However, in the systems of the above-mentioned patent documents and the like, since the threshold is fixed, there are many cases where colonies cannot be detected accurately.

  That is, in order to detect colonies accurately from a medium such as a film-type medium, including colony detection by visual inspection of workers, it is necessary to consider the relative relationship with the color of the medium. In the systems of the above-mentioned patent documents, even if the color of the medium fluctuates in each medium, pixels that constitute a colony (that is, colony pixels) are always detected by a certain threshold value. Since colonies are detected, there is a high possibility of causing false detection.

  The present invention has been made to solve the above-described problems, and its purpose is to accurately detect colonies even when the color of the medium fluctuates, such as individual differences or the occurrence of colonies. It is to provide a medium information registration system and the like that can be used.

  In order to solve the above-described problems, the culture medium information registration system and the like according to the present invention include an acquisition unit that acquires, as culture medium image data, medium data obtained by imaging a culture medium in which a colony is cultured. Extracting means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the medium image of the culture medium image data, and specifying the color feature amount having a predetermined condition from the color feature amount of the extracted pixel However, based on the specified color feature amount, a non-colony pixel different from the colony pixel used for imaging the colony is specified, and the color feature amount of the non-colony pixel is determined from the specified non-colony pixel. A specifying means for specifying, a pixel having a color feature amount having a predetermined color difference from the color feature amount of the specified non-colony pixel A configuration comprising: a detection unit that detects a colony pixel and detects the colony based on the detected colony pixel; and a registration unit that registers information about the colony including the detected colony in a database as medium information. have.

  With this configuration, the culture medium information registration system and the like according to the present invention specify the color feature amount of the pixel where the colony is not formed for each culture medium (that is, the color feature amount of the culture medium itself), and Since the colony pixels constituting the colony can be identified based on the feature value of the color of the pixel, individual differences in the color of the culture medium in which the colony is cultured, the influence of the imaging environment when the culture medium is imaged, and the colony Even when the color of the culture medium varies, such as when the color of the culture medium after culturing varies depending on the occurrence of colonies, colony pixels can be detected according to the variation.

  Therefore, the culture medium information registration system and the like according to the present invention can accurately detect colonies in any situation, and can register culture medium information including information related to accurately detected colonies in the database.

  In addition, the culture medium information registration system and the like according to the present invention can save resources as compared with the case where various data analysis and provision are performed using physical resources such as paper media, and the culture medium. By registering the culture medium information in the database, registration mistakes can be remarkably reduced and difficult medium management can be facilitated, so that resource saving can be achieved without wastefully consuming the culture medium.

  The medium information registration system and the like according to the present invention can accurately detect a colony in any situation, and can register medium information including information on the accurately detected colony in a database.

  Further, since the culture medium information registration system and the like according to the present invention can be digitized by registering the culture medium information in the database, it is possible to save resources and save the medium without wastefully consuming it. Recycling can be achieved.

It is a lineblock diagram showing the composition of one embodiment of the hygiene management system concerning the present invention. It is an example of the film type culture medium used for the sanitary management system of one Embodiment. It is an example of the work instruction used for the sanitary management system of one embodiment. It is a block diagram which shows the structure of the portable communication terminal device used for the sanitary management system of one Embodiment. It is a block diagram which shows the structure of the server apparatus used for the sanitary management system of one Embodiment. In one Embodiment, it is a figure for demonstrating that there exists an individual difference about the color of the culture medium itself which culture | cultivates a colony. It is a figure for demonstrating the operation principle of each process performed in the colony detection determination part of one Embodiment. It is FIG. (1) for demonstrating the detection process of the colony pixel in the colony detection determination part of one Embodiment. It is FIG. (2) for demonstrating the detection process of the colony pixel in the colony detection determination part of one Embodiment. It is a figure for demonstrating the histogram produced | generated in the colony detection determination part 422 of one Embodiment, and a non-colony pixel feature-value specific process. It is a figure for demonstrating the butterflies distance calculated in the colony detection determination part 422 of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the portable communication terminal device of one Embodiment. It is a flowchart which shows the operation | movement of the registration process of the culture medium information in the server apparatus of one Embodiment. It is a flowchart which shows the operation | movement of the colony detection determination process in the server apparatus of one Embodiment. It is the figure which imaged the color feature-value of the non-colony pixel specified in the culture medium image which should detect a colony in one Embodiment.

  Hereinafter, embodiments according to the present invention will be described with reference to the drawings. In addition, the following embodiment is a medium information registration system, program, and the like according to the present invention for a hygiene management system using a film-type medium in a food production line or an inspection line (hereinafter referred to as “work line”). It is embodiment at the time of applying a colony detection apparatus and a hygiene management system. However, the present invention is not limited to the following embodiments within the scope including the technical idea.

[1] Hygiene management system First, the hygiene management system S of this embodiment will be described with reference to FIG. In addition, FIG. 1 is a block diagram which shows the structure of the sanitary management system S of this embodiment.

  The sanitary management system S of the present embodiment uses a film-type culture medium (that is, a film-type culture medium) and performs data management on information on the culture medium (hereinafter referred to as “medium information”), thereby producing food. Or it is a system for inspecting the sanitary condition on inspection.

  In particular, the sanitary management system S of the present embodiment extracts a processed or processed food as a specimen in a work line 80 having a manufacturing process for manufacturing a processed or processed food or an inspection process for inspecting the food. In addition, this is a system for managing data on the culture state when a pre-specified bacterium such as a general bacterium or E. coli or the like occurring in a specimen in the film-type medium 60 is cultured.

  The hygiene management system S of the present embodiment manages and determines the sanitary state of food on the data, information relating to work in food production or inspection (hereinafter referred to as “work information”), and the extracted sample. Are registered in the server device 40 (specifically, the database 400) in association with the culture medium information of the film-type culture medium 60 acquired by the portable communication terminal device 10 before and after culturing the bacteria (colony) generated in It has a possible configuration.

Specifically, the sanitary management system S of the present embodiment is
(1) Pixels used to image a colony (hereinafter referred to as “colony” based on the feature amount of each pixel constituting a medium image (specifically, a predetermined target region) in which the film-type medium 60 is imaged. While specifying a pixel different from “pixel”), that is, a pixel constituting the color of the medium of the film-type medium 60 on the medium image (hereinafter referred to as “non-colony pixel”),
(2) Detecting colonies while detecting colony pixels on the medium image based on the color feature amount of the specified non-colony pixels,
It has a configuration.

  In order to realize such a configuration, the sanitary management system S of the present embodiment, as shown in FIG. 1, includes a plurality of portable communication terminal devices 10 used when registering culture medium information regarding each film-type culture medium 60. In addition, the administrator terminal device 20 that manages the sanitary management system S, the network 30, and the database 400, and in conjunction with the portable communication terminal device 10 or the administrator terminal device 20, registration management of medium information is performed. And a server device 40 that executes various types of processing.

  The work information of this embodiment includes, in addition to various types of information related to the manufacturing process or the inspection process, information regarding the culture of the food extracted from the manufacturing process or the inspection process as a specimen and the colonies generated in the specimen. In addition, it is registered in the database 400 using unique identification information (hereinafter referred to as “work ID”).

Specifically, each work information includes
(1) Work ID,
(2) Identification information (hereinafter referred to as “line ID”) of the work line 80 from which the sample is to be extracted or, if the work line 80 has a plurality of processes, the line ID and process identification information (hereinafter referred to as “line ID”). , Referred to as “process ID”) 71b;
(3) Product name of food as a sample, identification information of lot (hereinafter referred to as “lot ID”), lot name,
(4) Type of bacteria detected from specimens such as general bacteria or E. coli (hereinafter referred to as “test type”);
(5) Sample culture conditions such as dilution factor, type of diluent, culture temperature, culture humidity, and culture time,
(6) Specimen culture start time;
(7) a time after a predetermined time has elapsed since the start of culture of the specimen (hereinafter also referred to as “culture test time”);
(8) Sample incubator location such as incubator number and number of stages in incubator,
(9) Work date and time indicating the work start time and end time;
(10) Worker identification information (hereinafter referred to as “worker ID (employee ID)”) and worker name;
Is included. Each piece of work information is registered in the database 400 for each type and for each work.

  The portable communication terminal device 10 is, for example, a digital camera having a communication function, a tablet-type information terminal device, a smartphone or a portable telephone, or the like. And a communication terminal device portable by an operator.

  In particular, the portable communication terminal device 10 generates an image data (hereinafter referred to as “medium image data”) by imaging the film-type culture medium 60 by the camera function, and also images the work instruction sheet 70 and displays the instruction sheet. It has a configuration capable of generating an image.

  Then, the portable communication terminal device 10 acquires the work ID 71 and the culture medium ID 66 from the image data of the instruction manual image (hereinafter referred to as “instruction data image data”) and the culture medium image data of the film type culture medium 60, The acquired work ID 71 and medium ID 66, medium image data, and metadata of the medium image data can be registered in the database 400 of the server device 40 as medium information.

  On the other hand, when the portable communication terminal device 10 transmits the culture medium information to the server device 40, BLUETOOTH (registered trademark), wireless LAN (WLAN: Wireless Local Area Network), or wireless PAN (WPAN: Wireless Personal Area Network). The data is transmitted to the server device 40 directly or via the access point 50 using a communication standard for short-range wireless communication or the like, or is transmitted to the server device 40 using a public telephone line network via a mobile base station (not shown). It has a configuration.

  The portable communication terminal device 10 has a browser function constructed by a markup language such as XML (extensible Markup Language), and executes an operation input instruction and an operation confirmation by the operator using the browser function. The culture medium information is transmitted to the server device 40 via the browser function.

  The administrator terminal device 20 is an information communication terminal device such as a tablet information terminal device, a smartphone, a personal computer, or a workstation. The administrator terminal device 20 manages the administrator identification information (hereinafter referred to as “manager ID”), the administrator password, and the worker ID, manages the access authority to the server device 40, and is portable. It functions as a control device capable of performing management of the terminal ID of the communication terminal device 10, correction of registered medium information, and other management.

  Similarly to the portable communication terminal device 10, the administrator terminal device 20 has a browser function constructed by a markup language, and uses the browser function to exchange data with the server device 40 and to generate a report. It has a configuration capable of executing browsing and the like.

  Note that the administrator terminal device 20 determines the culture medium information when each operation is interrupted due to a failure of a machine that performs the process or the like in the same lot, or when an error in registration of culture medium information occurs in the operator. It has a configuration that can correct other information.

  The network 30 includes, for example, a public telephone line network (hereinafter referred to as “long-distance communication network”) including a mobile phone network, an IP (Internet Protocol) network such as a short-distance wireless network, or both of which are interconnected. Has been. However, the configuration of the network 30 is not limited to this.

  The server device 40 is a server device used in conjunction with the portable communication terminal device 10 or the administrator terminal device 20 to execute each data process for hygiene management of the lot and work line 80.

  Then, the server device 40 is linked with the portable communication terminal device 10, registers the culture medium information in each film-type medium 60 before the start of culture (hereinafter referred to as “initial registration”), and starts the culture in the database 400. While performing subsequent registration (hereinafter referred to as “post-cultivation registration”), the medium information transmitted from the portable communication terminal device 10 is acquired, and from the medium image formed by the medium image data of the acquired medium information In addition, it has a configuration for detecting colonies generated and cultured in a specimen.

Specifically, the server device 40
(1) Acquire media image data
(2) Extracting a color feature amount relating to the color of each pixel constituting a predetermined area in the culture medium image of the acquired culture medium image data,
(3) Specify a non-colony pixel based on the extracted color feature amount of each pixel, specify the color feature amount (representative value) of the non-colony pixel from the specified colony pixel,
(4) Detecting a colony based on the detected colony pixel while detecting a pixel having a color feature amount (representative value) of the specified non-colony pixel and a color feature amount having a predetermined color difference,
(5) Register information about the colonies including the detected colonies in the database as medium information;
It has a configuration.

  Further, in the process (3), the server device 40 classifies each gradation value and class when the color feature amount such as the luminance value, brightness, saturation, or RGB color component is defined by the gradation value. Based on a predetermined reference value (that is, a predetermined color feature amount) such as a reference value for binarization or a threshold for binarization, the color component of each pixel is classified into a plurality of types, The non-colony pixels are specified based on the classified result.

In particular, the server device 40 is configured to identify the non-colony pixels based on the number of pixels in each reference value. Specifically,
(3A) The number of pixels is counted for each reference value (for example, for each gradation value) for the color feature amount of each pixel extracted,
(3B) Based on the number of pixels of each reference value, specify a reference value having a predetermined condition, that is, a color feature amount,
(3C) specifying a non-colony pixel based on the specified color feature,
(3D) The color feature amount of the non-colony pixel is specified from the specified non-colony pixel.

  Note that the server device 40 replaces the processing of the above (3) with two classes of color feature values of each extracted pixel based on a predetermined reference value or according to a predetermined condition. And non-colony pixels may be specified based on the classified result.

  For example, the server device 40 classifies the extracted feature amount of each pixel into two classes by the k-means method, and specifies the classified pixel of the one class as a non-colony pixel. Further, for example, the server device 40 calculates an intermediate value based on the extracted maximum value and minimum value of the color feature amount of each pixel, binarizes each pixel using the intermediate value as a threshold value, and sets one value. The pixel having is identified as a non-colony pixel.

  In addition, the server device 40 according to the present embodiment counts the number of pixels for each color feature amount as the color feature amount satisfying a predetermined condition, and the color feature amount that is the mode value with respect to the number of pixels of each color feature amount. May be used.

  With such a configuration, in the sanitary management system S of the present embodiment, while specifying the color feature amount of the pixel where the colony is not formed for each medium (that is, the color feature amount of the non-colony pixel), Since the colony pixels constituting the colony can be specified based on the color feature amount of the non-colony pixel, individual differences in the color of the culture medium in which the colony is cultured, the influence of the imaging environment when imaging the culture medium, and Even when the color of the medium varies, such as when the color of the medium after the colony culture varies depending on the state of colony generation, colony pixels can be detected according to the variation.

  Therefore, in the sanitary management system S of the present embodiment, it is possible to accurately detect colonies in any situation and to register medium information including information related to the accurately detected colonies in the database. ing.

  Further, in the sanitary management system S of the present embodiment, it is possible to save resources as compared with the case where various data analyzes and provisions are performed using physical resources such as paper media, and the culture medium. By registering the culture medium information in the database 400, registration mistakes can be remarkably reduced, and management of difficult culture media can be facilitated. Therefore, resource saving can be achieved without consuming wasteful culture media.

[2] Film Type Medium Next, the film type medium 60 of the present embodiment will be described with reference to FIG. FIG. 2 is an example of a film-type medium 60 used in this embodiment.

  The film-type medium 60 used in the present embodiment is a medium for culturing bacteria generated in food as a specimen detected from each step of the work line 80 using a film or sheet-like dry medium. For example, the film-type medium 60 is used as a medium for culturing general live bacteria, coliforms, and Staphylococcus aureus. In addition, you may use as a culture medium which culture | cultivates various bacteria, substances, or microorganisms, such as mold | fungi, yeast, a Listeria bacterium, a microorganism for water quality, lactic acid bacteria, and a protein.

  The film-type medium 60 includes, for example, a base sheet 61 formed of a film and a circular frame formed on the base sheet 61 with reference to the center of the base sheet 61 as shown in FIG. (Hereinafter referred to as “circular frame”) 62, a culture layer 63 for culturing bacteria provided in the frame, a cover sheet 64 covering the culture layer 63, and the right side of the base sheet 61. Medium ID66.

  For example, the culture medium ID 66 is formed of a barcode such as a two-dimensional barcode or alphanumeric characters, and is imaged together when the film-type culture medium 60 is imaged, such as a barcode recognition function or an OCR (Optical Character Recognition) function. It is acquired in the portable communication terminal device 10 or the server device 40 using the image analysis function.

  The base material sheet 61 is not particularly limited as long as it is a film-like or sheet-like base material, and for example, a plastic film or paper can be used. Preferred examples of plastic films include resin films such as polyethylene, polypropylene, polyethylene terephthalate, polyethylene naphthalate, polymethacrylate, polymethyl methacrylate, polymethyl acrylate, polyester, and polycarbonate. Preferred examples include polyethylene terephthalate and polypropylene synthetic paper. Note that the polypropylene-based synthetic paper is a film synthetic paper whose main raw material is polypropylene.

  The film-type culture medium 60 has an IC tag in which a culture medium ID 66 is stored. The short-range wireless communication interface 140 (for example, an IC tag reader) included in the portable communication terminal apparatus 10 causes the medium to be connected to the portable communication terminal apparatus 10. ID66 may be acquired.

[3] Work instruction sheet Next, the work instruction sheet 70 of this embodiment will be described with reference to FIG. FIG. 3 is an example of a work instruction sheet 70 used in the present embodiment.

  The work instruction sheet 70 of the present embodiment includes, for each food group manufactured or inspected under the same conditions, that is, for each lot, work names and contents of one or more manufacturing processes or inspection processes, and work lines 80 to be used. It is an instruction document that describes the name of the food (processed food) to be manufactured or inspected, the quantity, and other information.

  Further, the work instruction sheet 70 of the present embodiment has an instruction sheet ID 71a formed by printing or other methods on the upper right and a plurality of process IDs 71b formed by printing or other methods on a predetermined area. . The instruction sheet ID 71a and each process ID 71b are used as the work ID 71, and are formed of, for example, a barcode such as a two-dimensional barcode or alphanumeric characters. The instruction ID 71a and each process ID 71b are imaged in the same manner as the film-type medium 60 when the registration of the film-type medium 60 is executed, and analyzed by the portable communication terminal device 10 or the server device 40. Be recognized.

  The work instruction sheet 70 has an IC tag in which the work ID 71 of the instruction sheet ID 71a or the process ID 71b is stored, like the film type culture medium 60, and has an interface (short distance) that the portable communication terminal device 10 such as a tag reader has. The portable communication terminal device 10 may acquire the work ID 71 by the wireless communication interface 140). In the following description, unless otherwise specified, the work ID 71 indicates an instruction ID 71a or a process ID 71b.

[4] Portable Communication Terminal Device Next, the configuration of the portable communication terminal device 10 of the present embodiment will be described using FIG. FIG. 4 is a block diagram showing the configuration of the portable communication terminal device 10 of the present embodiment.

  The portable communication terminal device 10 of the present embodiment has a data storage unit 100 having a memory function used when various programs are executed, an imaging function, image data of the film-type medium 60, and other image data. In addition to the image data generation unit 110 that generates the media information, the media information is registered in the server device 40 after the initial registration and the post-cultivation registration immediately after the culture in conjunction with the server device 40 (hereinafter referred to as “medium information registration processing”). And an application control unit 120 that executes processing.

  The portable communication terminal device 10 includes a network communication unit 130 that communicates with the server device 40 and other communication devices, a short-range wireless communication interface 140 that exchanges data with an IC tag and other communication interfaces, Controls the current position detection unit 150 that detects the current position, the display unit 160, the display control unit 161 that controls the display unit 160, the operation unit 170 for inputting user operations, the timer 180, and the entire apparatus. A portable terminal management control unit 190.

  And the portable communication terminal device 10 has various necessary members such as a microphone, a speaker, and an e-mail transmission / reception function when having a mail function such as a telephone function and e-mail. Further, the above-described units are connected to each other by a bus 11 to exchange data.

  The data storage unit 100 includes an application storage unit 101 that stores various application programs, an image data storage unit 102 that stores image data captured and generated by the image data generation unit 110, and a portable communication terminal device 10 and management / control programs, and a ROM / RAM 103 that is used as a work area during execution of each program and stores data used in each process executed by the portable communication terminal device 10. doing.

  In particular, the application storage unit 101 includes an application program (hereinafter referred to as “application”) that is executed by the application control unit 120 in conjunction with the image data generation unit 110, the operation unit 170, the display control unit 161, and the image data storage unit 102. Is stored). The application storage unit 101 also stores a browser program for realizing the browsing function described above.

  In the image data storage unit 102, the imaged medium image data and instruction sheet image data, the image ID for managing each image data, various metadata corresponding to each image data such as the imaging time, and the work ID 71 The medium ID 66 and various flag information are stored in association with each other. The image ID is arbitrary identification information that is appropriately given in each portable communication terminal device 10.

  The image data generation unit 110 includes an optical system, a CCDI sensor (Charge Coupled Device Image Sensor) that converts an optical image input from the optical system into an electrical signal, and image data based on the electrical signal generated by the CCDI sensor. And a generating unit that generates

  In particular, when the work instruction sheet 70 is imaged, the image data generation unit 110 converts the imaged work instruction sheet 70 into an image and generates instruction sheet image data. The image data generation unit 110 images the film-type medium 60 that has been imaged when the bacteria generated in the specimen are cultured or the film-type medium 60 that has been cultured is imaged. 60 pieces of medium image data are generated as medium image data.

  The application control unit 120 realizes the medium information registration process by a predetermined application. In particular, the application control unit 120 executes the network communication unit 130 and the display control unit 161 while executing various control programs for controlling each unit of the portable communication terminal device 10 using the culture medium registration application stored in the application storage unit 101. In addition, various processes are executed in conjunction with or controlled by the operation unit 170.

  Specifically, the application control unit 120 executes the medium registration application recorded in the application storage unit 101, controls the image data generation unit 110, acquires the medium image data of the film-type medium 60 captured. The image data is stored in the image data storage unit 102.

  Then, the application control unit 120 acquires the culture medium ID 66 from the imaged culture medium image data, and stores the culture medium image data, the metadata of the culture medium image data, and various flag information together with the acquired culture medium ID 66. Store in the unit 102. Note that the application control unit 120 may execute barcode recognition or OCR recognition in conjunction with another communication device or the database 400 via a communication line.

  In addition, the application control unit 120 performs initial registration for registering the culture medium information in the server device 40 at the start of the culture of the specimen after imaging of the culture medium image data or at a predetermined timing, and registers the culture medium information after the start of the culture of the specimen. Perform registration after incubation. In particular, the application control unit 120 identifies whether the registration is initial registration or post-cultivation registration based on the operator's instruction, and includes information (flag information) indicating either registration in the culture medium information in the server device 40. Send.

  The application control unit 120 acquires the current time from the timer 180 when the film-type medium 60 is imaged, and the image data storage unit as the metadata of the medium image data together with a predetermined image ID using the current time as the imaging time. 102. When the medium image data is transmitted to the server device 40, it is transmitted together.

  In addition, the application control unit 120, when the culture medium ID 66 and the work ID 71 that is the instruction ID 71a and the process ID 71b are stored in the IC tag, the operation unit 170, the display control unit 161, and the short-range wireless communication interface The medium ID 66 and the work ID 71 are acquired in conjunction with 140.

  When the server device 40 recognizes the medium ID 66 and the work ID 71 from the medium image data and the instruction sheet image data, the application control unit 120 transmits instruction sheet image data in addition to the medium image data as the medium information.

  Furthermore, the application control unit 120 may be realized as a function when the CPU (central processing unit) constituting the mobile terminal management control unit 190 executes the application in terms of implementation.

  The network communication unit 130 establishes a communication line with the server device 40 connected to the network 30 under the control of the application control unit 120 and the mobile terminal management control unit 190, and exchanges various data such as culture medium image data. Do.

  The short-range wireless communication interface 140 performs short-range wireless communication using an IC tag under the control of the application control unit 120 and the mobile terminal management control unit 190.

  The current position detection unit 150 recognizes the position of a GPS (Global Positioning System) satellite via the network 30 under the control of the application control unit 120 or the mobile terminal management control unit 190, and transmits a satellite transmitted from the GPS satellite. A signal (GPS signal) is detected.

  Then, the current position detection unit 150 calculates (that is, detects) coordinate values indicated by the latitude and longitude of the current position of the portable communication terminal device 10 based on the detected GPS signal. In addition, the current position detection unit 150 provides the calculated coordinate value to the application control unit 120 as position information.

  In addition, when the portable communication terminal device 10 has a telephone function or a short-range wireless function, the portable communication terminal device 10 is based on the direction and the radio field intensity received by the telephone base station or the like for the radio wave used for the telephone or the short-range radio. The current position of the portable communication terminal device 10 may be calculated (detected).

  The display unit 160 has an image display area of a predetermined size (for example, 5 inches, W480 × H960 pixels), is configured by a panel of liquid crystal elements or EL (Electro Luminescence) elements, and is generated by the display control unit 161. A predetermined image is displayed based on the display data. In particular, in the present embodiment, when the culture medium registration application is being executed, the display unit 160 is linked to the operation unit 170, and various displays, captured work instructions 70 and film-type culture media that have been imaged. 60 is displayed.

  The display control unit 161 generates drawing data necessary to cause the display unit 160 to draw a predetermined image under the control of the application control unit 120 or the mobile terminal management control unit 190, and the generated drawing data is displayed on the display unit. 160 is output.

  The operation unit 170 includes various confirmation buttons, operation buttons for inputting operation commands, a number of keys such as a numeric keypad, and a touch sensor provided on the display unit 160, and is used when performing each operation. It has become. Specifically, the operation unit 170 is used when performing operations for executing the various processes described above when the culture medium registration application is activated.

  In the present embodiment, the operation unit 170 can also be used when manually inputting the culture medium ID 66 directly.

  The timer 180 provides the application control unit 120 with the date and time when the image data generation unit 110 images the film-type medium.

  The portable terminal management control unit 190 is mainly configured by a central processing unit (CPU) and includes various input / output ports such as a key input port and a display control port, and the overall functions of the portable communication terminal device 10. In addition, overall functions for executing the information providing program are generally controlled.

[5] Server Device [5.1] Configuration of Server Device Next, the configuration of the server device 40 of this embodiment will be described with reference to FIG. FIG. 5 is a configuration diagram showing the configuration of the server device 40 of the present embodiment.

  As shown in FIG. 5, the server device 40 of the present embodiment communicates with a database 400 in which various information such as work information and culture medium information is stored, and the portable communication terminal device 10 and the administrator terminal device 20. A communication control unit 410, a data processing unit 420 that executes various processes such as a culture medium information registration process, a server management control unit 430 that controls each unit of the server device 40, a ROM / RAM 440 that is used to control each unit, and a time And a timer 450 used for management. The above-described units are connected to each other by a bus 41, and data transfer is performed between the components.

  The communication control unit 410 is a predetermined network interface, establishes a communication line with the portable communication terminal device 10 or the administrator terminal device 20, and exchanges various data with the portable communication terminal device 10 or the administrator terminal device 20. I do.

  The database 400 includes an HDD or an SSD, and includes a medium information database (hereinafter abbreviated as “medium information DB”) 401, a line information database (hereinafter abbreviated as “line information DB”) 402, and a lot information database (hereinafter referred to as “line information DB”). , “Lot information DB”) 403 and a work management database (hereinafter abbreviated as “work management DB”) 404. For example, each DB of the present embodiment constitutes a database according to the present invention.

The medium information DB 401 is a database in which medium information regarding the film-type medium 60 of the sample extracted for each process of each work line 80 is stored for each work ID 71 and medium ID 66. For example, in the culture medium information DB 401,
(1) Medium ID
(2) Imaged medium image data (3) Image capture time (4) Other information including flag information (5) Colony candidate specific information including feature amount relating to color of each colony candidate (6) Colony candidate imaged Information of each colony candidate (hereinafter referred to as “colony candidate information”) including the feature quantity of the detection pixel used for the detection and the coordinate position of the film-type medium 60 (hereinafter referred to as “colony candidate information”). ID) and the number of colonies (8) Results of medium determination for determining whether the film-type medium 60 (ie, specimen) is normal or abnormal based on the number of colonies (9) Work ID
Are recorded in association with each other.

  When the sample to be extracted is always extracted from the same process on the same work line 80 in the same lot, the work ID may be a lot ID, and the sample to be extracted is the same lot. However, when extracting from a different work line 80 or a different process, the line ID or the process ID 71b is obtained.

  The number of colonies and the result of medium determination in each medium information may be registered when the medium information is registered, or may be registered at a predetermined timing different from the registration timing of the medium information.

  Furthermore, when a plurality of imaging times of medium information of the same film type culture medium 60 are registered (that is, when there are a plurality of culture examination times described later), in the same culture medium ID 66, the culture medium at each culture examination time. Each medium information of an image, imaging time, the number of colonies, and medium determination is stored.

The line information DB 402 is a database in which line information regarding each work line 80 is stored for each line ID. For example, the line information DB 402 includes
(1) Line ID
(2) The process ID 71b of the process included in the work line 80 and the two types of data are recorded in association with each other. As the process type, a name for identifying each process such as material input, blending, filling, boiling, packaging, and packaging is used.

The lot information DB 403 is a database in which lot information regarding each lot is stored for each lot ID. For example, in the lot information DB 403,
(1) Lot ID (instruction ID 71a)
(2) Line ID of the work line used for lot production or inspection
(3) Lot work start time (4) Lot work end time (5) Five data of the work teams of the workers who perform the lot work are recorded in association with each other.

The work management DB 404 is a database that stores various types of information related to work contents. For example, the work management DB 404 includes
(1) Work ID
(2) Line ID (or line ID and process ID 71b) of the work line from which the sample is to be extracted (3) Type of sample (food) (product name, lot ID and / or lot name)
(4) Type of inspection (generic bacteria or bacteria to be detected such as E. coli)
(5) Culture conditions (dilution ratio, type of diluent, culture temperature, culture humidity and culture time)
(6) Culture start time (7) Time after a predetermined time has elapsed from the start of culture (hereinafter also referred to as “culture test time”)
(8) Culture location (for example, the position of the incubator number and the number of steps in the incubator)
(9) Work date and time (work start time and end time)
(10) Worker ID (and / or worker name)
Are recorded in association with each other.

  In addition, only a single time (that is, an end time for ending the culture of the specimen) may be set as the culture examination time, or after the statutory or predetermined time has elapsed from the culture start time, for example, the culture A plurality of times such as 24 hours from the start time, 48 hours, or 72 hours may be set.

The data processing unit 420 executes various data processing in accordance with the application recorded in the ROM / RAM 440. In particular, the data processing unit 420 executes a predetermined program,
(1) Operation management of the communication control unit 410;
(2) Medium information registration processing for acquiring various medium information in cooperation with each portable communication terminal device 10 and registering it in the database 400;
(3) A colony is detected from each medium image of each film type medium 60 during the medium information registration process or at a predetermined timing after the medium information registration process, the colonies are counted, and the medium is based on the number of colonies A colony detection determination process for executing the determination;
(4) lot determination processing for determining a lot based on registered medium information;
(5) Report processing for outputting the determination result in a report format;
(6) Management and control of the database 400;
I do.

  In particular, in the colony detection determination process, the data processing unit 420 specifies the color feature amount of the non-colony pixel indicating the color of the medium for each film-type medium 60, and based on the specified color feature amount of the non-colony pixel. While detecting colony pixels, colony detection and counting are performed.

  Specifically, the data processing unit 420 includes a registration processing unit 421 that executes a medium information registration process including management and control of the database 400, a colony detection determination unit 422 that executes a colony detection determination process, and a lot determination process. A lot determination processing unit 424 that executes and a report processing unit 425 that executes reporting processing.

  For example, the registration processing unit 421 of the present embodiment constitutes an acquisition unit and a registration unit according to the present invention, and the colony detection determination unit 422 of the present embodiment includes the extraction unit, the identification unit, and the detection unit of the present invention. Configure. Further, details of the registration processing unit 421, the colony detection determination unit 422, the lot determination processing unit 424, and the report processing unit 425 of this embodiment will be described later.

  The server management control unit 430 is mainly configured by a central processing unit (CPU), and integrally controls each unit of the server device 40 by executing a program. Specifically, the server management control unit 430 performs login processing for each user and various other controls based on a login request from the portable communication terminal device 10 by a user operation.

  Various programs necessary for driving the server device 40 are recorded in the ROM / RAM 440. In particular, in the ROM / RAM 440, information for defining the reference characteristics, medium determination (pass / fail determination) of each film type medium 60, determination criteria used when executing each determination of process determination, line determination, and lot determination. Each information such as information (hereinafter also referred to as “medium determination criterion information”) is stored. The ROM / RAM 440 is used as a work area during execution of each program.

  The timer 450 is used for managing the time required when the medium information registration process and the lot determination process are executed.

[5.2] Registration Processing Unit Next, details of the registration processing unit 421 in the server device 40 of the present embodiment will be described.

  The registration processing unit 421 according to the present embodiment performs a medium information registration process for registering each medium information in the medium information DB 401 while being associated with the work ID 71 in conjunction with the portable communication terminal device 10. In particular, the registration processing unit 421 executes different medium information registration processing based on flag information indicating whether the registration is initial registration or post-culture registration included in the culture medium information.

(Initial registration)
When the flag information indicating initial registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 performs a culture medium information registration process based on the initial registration.

  Specifically, when the registration processing unit 421 acquires the culture medium information via the communication control unit 410, the registration processing unit 421 specifies the culture medium ID 66 and the work ID 71 included in the culture medium information. In addition, the registration processing unit 421 searches the work management DB 404 based on the specified work ID 71, specifies the corresponding work information, and associates the acquired medium image data and imaging time with the specified work information. Is registered in the culture medium information DB 401.

  In particular, the registration processing unit 421 identifies the lot ID including the work line 80 from which the sample is extracted based on the acquired work ID 71, the line ID of the work line 80, the process ID 71b from which the sample is extracted, and the identification. The medium image data and the imaging time included in the medium information are registered in the medium information DB 401 in association with the lot ID, the line ID, and the process ID 71b.

  Then, the registration processing unit 421 registers the imaging time in the culture medium information DB 401 as the culture start time. As the culture start time, a preset time may be used instead of the imaging time on the assumption that the medium information registration process is executed at the time.

(Registration after culture)
When the flag information indicating post-cultivation registration is included in the culture medium information transmitted from the portable communication terminal device 10, the registration processing unit 421 performs a registration process based on post-cultivation registration.

  Specifically, when acquiring the culture medium information via the communication control unit 410, the registration processing unit 421 identifies the culture medium ID 66 and the work ID 71 included in the culture medium information, as in the initial registration. Then, the registration processing unit 421 searches the work management DB 404 based on the identified work ID 71, identifies the corresponding work information, and registers the imaging time in the culture medium information DB 401 in association with the identified work information. To do. Note that the culture examination time may be a preset time instead of the imaging time on the assumption that the culture information registration process is executed at the same time as the culture start time.

  On the other hand, the registration processing unit 421 registers the acquired medium image data in the medium information DB 401 together with the analysis result or without analysis while causing the colony detection determination unit 422 to perform image analysis. The colony detection determination unit 422 performs colony detection, colony count, and medium determination by image analysis, and details of the image analysis will be described later.

[5.3] Colony Detection Determination Unit [5.3.1] Principle of Operation of Colony Detection Determination Unit Next, the colony detection determination unit 422 in the server device 40 of the present embodiment will be described with reference to FIGS. The operation principle of will be described.

  6 is a diagram for explaining that there is an individual difference in the color of the culture medium itself for culturing colonies, and FIG. 7 is a diagram for explaining the operating principle of the colony detection determination unit 422 in the present embodiment. FIG. 8 and 9 are diagrams for explaining a colony pixel detection process in the colony detection determination unit 422 of the present embodiment.

  Usually, in order to detect colonies accurately from the film-type medium 60, including the colony detection by the worker's visual observation, the relative colors of the color of the film-type medium 60 itself and the color of the colonies cultured on the medium are used. It is also necessary to take into account such relationships.

  For example, if there is an individual difference in the color of the culture medium itself in which the colony is cultured, the influence of the imaging environment when imaging the culture medium, and the color of the culture medium itself after culturing the colony vary depending on the occurrence of the colony, The color of the medium may vary. In particular, as shown in FIGS. 6A, 6B, and 6C, even when the film-type medium 60 is formed of the same material, the color of the medium is not the same, and The color fluctuates.

  In addition, since the colony is formed on the medium, the color of the colony is often reflected in the color of the colony. In such a case, when the color of the medium changes, the color of the colony also changes. . In other words, colonies have the property that the intensity of color development increases as they are formed and grown on the medium, and changes in the color intensity appear in the formation process and formed colonies. As a result, the color of the medium is reflected in the color of the colony.

  In particular, since the area around the colony or when the colony does not grow sufficiently, an area with low color intensity called “smudge” is also formed. Reflected.

  Therefore, as shown in FIG. 7 (A), a threshold value for discriminating between a colony pixel and a non-colony pixel is fixed for each pixel extracted from the medium image, and a detection criterion for distinguishing the colony from the medium is fixed. If this happens, colony detection omission or false detection may occur, and it cannot be said that colonies can be detected accurately.

  Therefore, the colony detection determination unit 422 of the present embodiment determines, for each film-type culture medium 60, colonies based on the color feature amount of each pixel constituting a predetermined area (that is, the area of the culture layer 63) in the culture medium image. Identifies non-colony pixels that make up different non-colonies (ie, the medium itself), and detects colony pixels based on the color features (representative values) of the identified non-colony pixels, According to the detected colony pixel, the colony is detected and counted.

  That is, as shown in FIG. 7B, the colony detection determination unit 422 does not change the colony color even if the color of the non-colony varies for each film-type medium 60 and affects the color of the colony. Since the threshold value for identifying a non-colony can be changed, the colony can be accurately detected.

  For example, the colony detection determination unit 422 specifies the non-colony pixel (black portion) as illustrated in FIG. 8B while acquiring the medium image as illustrated in FIG. 8A. Based on the non-colony pixel, the color feature amount of the non-colony pixel as shown in FIG. Then, as illustrated in FIG. 9A, the colony detection determination unit 422 subtracts the feature amount of each pixel from the color feature amount of the non-colony pixel, and the subtraction result is as illustrated in FIG. 9B. A pixel having a predetermined value, that is, a feature amount having a predetermined color difference from the color feature amount of the non-colony pixel is detected as a colony pixel.

  8 and 9 are diagrams in which each process executed in the colony detection determination unit 422 is imaged.

  As described above, the colony detection determination unit 422 according to the present embodiment can detect colonies by detecting colony pixels based on the color of the medium for each film-type medium 60, and therefore, when the color of the medium fluctuates. Even if it exists, a colony can be detected accurately.

  Further, by having such a configuration, in the present embodiment, it is possible to accurately detect colonies in any situation, and to register medium information including information related to accurately detected colonies in the database. It can be done. Details of each process of the colony detection determination unit 422 will be described below.

[5.3.2] Details of Each Process of Colony Detection Determination Unit Next, details of each process of the colony detection determination unit 422 in the server device 40 of the present embodiment will be described with reference to FIGS. 10 and 11.

  FIG. 10 is a diagram for explaining the histogram and non-colony pixel feature amount specifying process generated in the colony detection determination unit 422 of the server device 40 of this embodiment, and FIG. 11 is the server of this embodiment. It is a figure for demonstrating the butterflies distance calculated in the colony detection determination part 422 of the apparatus 40. FIG.

  The colony detection determination unit 422 is controlled by the registration processing unit 421, and the medium image data included in the medium information transmitted from the portable communication terminal device 10 at the time of registration of the medium information in the medium information DB 401 or at a predetermined timing. Obtain media images from

  In addition, the colony detection determination unit 422 extracts the color feature amount of each pixel constituting the acquired medium image such as the RGB gradation value, luminance value, or saturation, and identifies the non-colony pixel while identifying the non-colony pixel. Specify the color feature of the pixel.

  Then, the colony detection determination unit 422 detects colony pixels based on the identified non-colony pixels, and based on the detected colony pixels, detects the colonies and counts them, and determines whether the specimen is acceptable based on the number of colonies. Are provided to the registration processing unit 421.

Specifically, the colony detection determination unit 422
(1) While specifying the region to be processed (the region of the culture layer 63) from the medium image of the medium image data, it relates to the color of each pixel constituting the region, such as the luminance, brightness, or RGB gradation value of each pixel. Color feature extraction processing for extracting feature quantities as color feature quantities,
(2) A non-colony pixel specifying process for specifying a non-colony pixel based on the extracted color feature while extracting a color feature having a predetermined condition based on the extracted color feature of each pixel.
(3) A specifying process for specifying a characteristic amount of a representative non-colony pixel from a plurality of specified non-colony pixels (hereinafter referred to as “non-colony pixel feature amount specifying process”),
(4) Detection processing (hereinafter referred to as “colony pixel detection processing”) for detecting a pixel having a color feature amount having a predetermined color difference from a color feature amount of a non-colony pixel as a colony pixel.
(5) Colony detection processing for counting the number of colonies while detecting colonies based on the detected colony pixels, and
(6) Medium determination process for performing medium determination based on the counted number of colonies,
Execute.

(Color feature extraction processing)
The colony detection determination unit 422 identifies the region to be processed (the region of the culture layer 63) from the medium image based on the position of the medium ID 66 and the like, and determines the luminance value, brightness, saturation, or sub for each pixel constituting the region. A preset color feature amount such as a gradation value of a pixel (RGB) is extracted. For example, when using a luminance value, the colony detection determination unit 422 extracts a gradation value indicating a luminance value from 0 to 255 in each pixel constituting a predetermined region in the culture medium image.

(Non-colony pixel identification process)
The colony detection determination unit 422 according to the present embodiment classifies the extracted color feature amount of each pixel into a plurality of types based on a predetermined reference value, and identifies a non-colony pixel based on the classified result. That is, the colony detection determination unit 422 counts the number of pixels for each reference value, and identifies a non-colony pixel based on the counted number of pixels.

  Specifically, the colony detection determination unit 422 uses a histogram of the number of pixels for each color feature amount (reference value) as the probability distribution of the color feature amount of the pixel (the color of the pixel having the color feature amount and the pixel number as coordinate axes). A histogram indicating a probability distribution in the feature amount is generated, and a color feature amount assumed to be a non-colony pixel is specified based on the generated histogram, and a non-colony pixel is specified based on the specified color feature amount.

  Usually, on the region of the culture layer 63 in the medium image, for example, a certain number or more of the total number of pixels, that is, a certain area or more (for example, at least one-half or more) of the entire area of the region is a colony. It is assumed that it is a non-colony pixel, not a pixel.

  Therefore, the colony detection determination unit 422 of the present embodiment is closest to the color feature amount of the colony pixel among the color feature amounts of the non-colony pixels as the color feature amount having a predetermined condition based on the generated histogram. A color feature amount assumed to be a color feature amount is specified, and a range of the color feature amount of a non-colony pixel is specified based on the specified color feature amount. And the colony detection determination part 422 specifies the pixel which belongs to the range of the color feature-value of a non-colony pixel as a non-colony pixel.

  In particular, the colony detection determination unit 422 sequentially accumulates the number of corresponding pixels according to a continuous change from the first reference value to the second reference value of the color feature amount for the generated histogram, and the accumulated pixel number A color feature amount at a preset value is specified as a reference feature amount.

  For example, when using the luminance value, the colony detection determination unit 422 classifies each pixel constituting the medium image into each luminance value from 0 to 255, and uses each color feature amount and the number of pixels as coordinate axes. A histogram as shown in FIG.

  Specifically, the colony detection determination unit 422 determines the color of the most colony as the second reference value from the color feature amount (luminance gradation value “255”) indicating the color of the medium itself as the first reference value. The number of pixels of each color feature amount is sequentially integrated up to the color feature amount indicating luminance (brightness gradation value “0” or the minimum gradation value when indicating a color as a colony such as R, G, or B). The color feature amount (for example, the color feature amount when the number of pixels is a half of the total number of pixels in the culture layer 63 region ( The gradation value)) is specified as the reference feature amount.

  Then, the colony detection determination unit 422 specifies the color feature amount from the first reference value to the reference feature amount as the range of the color feature amount of the non-colony pixel, and specifies the pixels belonging to the range as the non-colony pixel.

  Note that the colony detection determination unit 422 differs from the reference feature amount calculated by integrating the number of pixels as described above, in the generated histogram, the color feature amount having the mode number of pixels as a reference feature amount, The non-colony pixel may be specified by specifying the range of the color feature amount of the non-colony pixel based on the reference special feature amount in the same manner as described above.

  Further, the colony detection determination unit 422 extracts the minimum and maximum values of the color feature amount of each pixel in the area of the culture layer 63, binarizes the intermediate value between the minimum and maximum values as a threshold, A pixel having a value (for example, belonging to the maximum value side when the color feature amount is luminance) may be specified as a non-colony pixel.

  Then, the colony detection determination unit 422 uses the K-means method that can be classified into a specified number of classes without the need for teacher data based on the color feature amount of each pixel. ), And a pixel belonging to one class (for example, a class having a high average luminance when the color feature amount is luminance) may be specified as a non-colony pixel.

  Furthermore, the colony detection determination unit 422 may specify all the regions of the culture layer 63 in the medium image as non-colony pixels. Normally, in order to statistically identify the non-colony color, even if all colonies in the area are identified as non-colony pixels, there is almost no effect if the number of colony pixels is small, and there is no decrease in accuracy. At the same time, the processing speed can be improved.

(Non-colony pixel color feature identification processing)
The colony detection determination unit 422 specifies a color feature amount determined based on the color feature amount of each pixel identified as a non-colony pixel as a color feature amount (representative value) of the non-colony pixel.

  Specifically, the colony detection determination unit 422 uses the luminance value, brightness, or gradation value of the RGB color component as a color feature amount for each pixel identified as a non-colony pixel, and displays these values as a graph. The median value or the maximum value of the color feature amount at the time of conversion into the color mode, or the mode feature value or the average value color feature value with the maximum number of pixels is specified as the color feature amount (representative value) of the non-colony pixel.

  In addition, since there is no large blurring in the color of the non-colony pixels and the number of non-colony pixels is sufficiently large, the color feature amount of the non-colony pixels can be accurately determined using any of the median, mode, and average values. Can be identified as

  In addition, the colony detection determination unit 422, when calculating the color feature amount (representative value) of a non-colony pixel using a single color feature amount in each pixel such as a luminance value or brightness, is simply a median, maximum In the case of calculating a color feature amount (representative value) of a non-colony pixel using a plurality of color feature amounts such as gradation values of RGB color components, for example, each color feature amount ( That is, a combination of the median value, maximum value, mode value, or average value of each of RGB color components) is used.

  Further, the colony detection determination unit 422 may use the same color feature amount as the color feature amount used when specifying the non-colony pixels, or may use a different color feature amount. However, in order to calculate the color difference used when the colony pixel is detected, the colony detection determination unit 422 preferably uses the same color feature amount as the color feature amount used for the calculation of the color difference.

(Colony pixel detection process)
The colony detection determination unit 422 calculates the color difference between the color feature amount (representative value) of the identified non-colony pixel, such as the gradation value of each color component of RGB, and the feature amount of each pixel, and exceeds a predetermined color difference. A pixel having a color feature amount is detected as a colony pixel.

  Specifically, when using a plurality of color feature amounts such as RGB color components, the colony detection determination unit 422 includes a plurality of color components of each pixel specified as a non-colony pixel and a plurality of color components of each pixel. On the other hand, while generating a histogram for each color component gradation value, the similarity of the generated histogram is calculated as a color difference, and a colony pixel is detected based on the color difference calculated as the similarity. In particular, the colony detection determination unit 422 uses the batch coefficient and the batch distance to calculate the similarity in the histogram.

  For example, when the colony detection determination unit 422 uses a plurality of color feature amounts such as RGB color components and uses the batcher coefficient BC and the batcher distance DB, the RGB of each pixel specified as a non-colony pixel is used. While normalizing the color component p and the color component q of each pixel, the calculation shown in (Equation 1) and (Equation 2) is executed to detect a pixel having a batch rear distance of a certain value as a colony pixel.

  In addition, in the medium image as shown in FIG. 11A, the color portion of the medium is specified as a non-colony pixel, and each color component of RGB of the non-colony pixel is specified as a color feature amount (representative value). If it is, the colony detection determination unit 422 can detect the colony pixel based on the batcha rear distance as shown in FIG.

  Further, the colony detection determination unit 422 normalizes the RGB gradation values of each pixel and the non-colony pixels so that the sum of the RGB gradation values is “1”, and calculates a virtual coefficient.

  The batcher coefficient BC defines two or more histograms (that is, a histogram composed of tone values of the color components RGB in the above case) as a one-dimensional value, and the value is “0”. ”To“ 1 ”, and the higher the similarity, the higher the histogram. Further, the batcha rear distance DB is a value obtained by converting the batch rear coefficient into a distance, and it is determined that two histograms are more similar as the value is larger. Therefore, the colony detection determination unit 422 detects a colony pixel by calculating a buttery rear coefficient and converting it to a buttery rear distance.

  On the other hand, the colony detection determination unit 422, when using a single color feature amount such as a luminance value or brightness, performs Euclidean for the color component specified as the color feature amount of the non-colony pixel and the color component of each pixel. By calculating the distance or the Manhattan distance, a pixel having a color feature amount equal to or greater than a predetermined distance difference (that is, a color difference) is detected as a colony pixel.

  In addition, as a colony used when calculating the color difference from the color feature value (representative value) of the specified non-colony pixels, in principle, pixels that are not identified as non-colony pixels are targeted, but all pixels are targeted. It is good.

(Colony detection process)
The colony detection determination unit 422 performs ID filtering by performing connection and labeling of adjacent colony pixels while performing image filter processing for reducing noise based on the detected colony pixels, and connecting the colony pixel groups Are detected as one object, that is, a colony, and the number is counted as the number of colonies.

  In particular, the colony detection determination unit 422 includes an expansion process that replaces all the pixels adjacent to the colony pixel with colony pixels when there are other colony pixels adjacent to each colony pixel as the image filter process, and When there is a non-colony pixel adjacent to the colony pixel (hereinafter referred to as “non-colony pixel”), a colony is detected and the number of pixels detected while performing a contraction process to replace all adjacent pixels with non-colony pixels. Count.

In addition, the colony detection determination part 422 is for each detected colony.
(1) Various information of colony pixels included in the colony, such as the position of the colony pixel included in each colony, the feature amount relating to the color, and the arrangement position on the medium image, and
(2) Specify information related to colonies such as the position of the center of gravity of each colony on the medium image or the color feature amount (average).

  Then, the colony detection determination unit 422 provides various information including the number of colonies to the registration processing unit 421.

(Sample pass / fail judgment)
The colony detection determination unit 422 performs medium determination (that is, determination of pass / fail of the specimen) as to whether the specimen of each film type culture medium 60 is abnormal or normal. For example, the colony detection determination unit 422 determines pass / fail of each sample based on a threshold value of the number of bacteria set in advance according to the bacterial species to be detected from the sample (that is, the determination criterion), and the result is the registration processing unit 421. To provide.

  In particular, in the present embodiment, since the determination criteria are different based on the bacterial species and the dilution rate, the registration processing unit 421 obtains the medium determination criterion information from the ROM / RAM 440 based on the criteria used when cultivating the specimen and the bacterial species. Read out and perform medium determination.

  Each criterion information is specified in association with the culture medium ID 66. That is, since the type of the film-type medium 60 is different for each bacterial species detected from the specimen, the bacterial species to be detected can be specified by specifying the medium ID 66. Therefore, in this embodiment, the determination reference information specified based on the culture medium ID 66 can be read out by setting the dilution rate and the like in advance.

  Moreover, in this embodiment, since the pass / fail determination of each film type culture medium 60 is used in the process determination, the line determination, and the lot determination, the medium determination is executed in association with these determinations. In addition, the method of pass / fail determination of each film type culture medium 60 in process determination, line determination, and lot determination will be described later.

[5.4] Lot Determination Processing Unit Next, details of the lot determination processing unit 424 in the server device 40 of the present embodiment will be described.

  The lot determination processing unit 424 of the present embodiment analyzes data on hygiene management in the designated lot, work line 80 or process based on the instructions of the administrator input to the administrator terminal device 20 (ie, pass / fail determination). Execute.

  Specifically, the lot determination processing unit 424, for each lot, line information of the work line 80 used for the corresponding lot, and medium information registered in association with the corresponding work line 80 (at least the number of colonies). Based on the above, it is determined whether the food group manufactured or inspected under the same condition has a predetermined condition.

  In addition, when the lot determination is instructed, the lot determination processing unit 424 determines whether the work line 80 included in the corresponding lot and the processes belonging to the work line 80 are abnormal or normal in terms of hygiene management. 80 line determinations (that is, pass / fail determination for hygiene management) and process determinations for each process (that is, determination of pass / fail for hygiene management) are performed, and lot determination is performed using the process determination and line determination.

  In particular, when the lot determination is instructed, the lot determination processing unit 424, for example, the number of colonies of the film-type medium 60 in the medium information that has passed a predetermined culture inspection time such as 24 hours or 48 hours from the start of culture, Based on the medium information of the pass / fail determination of the specimen, the pass / fail of hygienic management of the process is determined, and the pass / fail of hygiene management of the work line 80 is determined based on the pass / fail determination of the process included in the corresponding work line 80. Based on the pass / fail determination of the work line 80 included in the corresponding lot, the pass / fail in sanitary management of the lot is determined.

  Note that the lot determination processing unit 424 of the present embodiment may execute not only lot determination but also process determination or line determination as data analysis.

(Process judgment)
The lot determination processing unit 424 has a culture start time at the same timing, a culture examination time at the same timing, a culture start time having a time within a preset time from the first time to the second time, or a preset first time. The culture start time or the culture test time has a predetermined condition, such as having a culture test time having a time within the time from the first time to the second time, and is registered in association with the same process in the same lot. Each process determination is performed based on the culture medium information of the plurality of film-type culture media 60 that has been performed. That is, the lot determination processing unit 424 executes each process determination by comprehensive determination based on each medium determination result of the plurality of film-type mediums 60.

  Specifically, the lot determination processing unit 424 displays the medium determination results of the corresponding film-type medium 60 (that is, all the medium determination results registered in the medium information having the process ID 71b of the process to be subjected to the process determination). In addition to obtaining, if the number of pass / fail of the judgment result satisfies a predetermined condition, the process judgment of the process to be judged is judged to be acceptable, and if not, the judgment should be made The process judgment of the process is judged as rejected.

  For example, the lot determination processing unit 424 determines that the conditions for all the film-type culture media 60 when the acceptable film-type culture media 60 are a predetermined ratio (60%) or more in the individual film-type culture media 60 in the corresponding process ( The number of colonies is equal to or less than a certain number), or the condition that the number of colonies of each film-type medium 60 corresponds (the average number of colonies of a single film-type medium 60 is equal to or less than a certain number). In this case, the corresponding process is determined to be acceptable.

  Note that the lot determination processing unit 424 of the present embodiment can change conditions depending on the type of specimen, the type of bacteria to be detected, etc., and can be set by an administrator or provided as part of a program. The

(Line judgment)
The lot determination processing unit 424 performs line determination for each work line 80 based on the pass / fail determination for hygiene management of a plurality of processes that are determined at a predetermined timing and registered in association with the corresponding line information. To do. That is, the lot determination processing unit 424 performs line determination by comprehensive determination based on the determination results of a plurality of processes of the same lot determined at a predetermined timing.

  Specifically, the lot determination processing unit 424 is determined at the same timing or a timing satisfying a predetermined condition, and has the line ID of the work line 80 to be subjected to line determination as described above. When the determination result of the determination is acquired and the number of pass / fail of the determination result satisfies a predetermined condition, the line determination of the work line 80 to be determined is determined to be acceptable, and the condition is not satisfied The line determination of the work line 80 to be determined is determined to be unacceptable.

  For example, when the three processes have the line ID of the work line 80 to be determined, the first process is “pass”, the second process is “pass”, and the third process is “fail”. In the case where the work line 80 is configured from a process in which the predetermined condition is “60%”, the lot determination processing unit 424 determines that the line determination of the work line 80 to be determined is acceptable.

(Lot judgment)
The lot determination processing unit 424 executes the lot determination for each lot based on the hygiene management pass / fail determination of the plurality of work lines 80 that are determined at a predetermined timing and registered in association with the corresponding lot information. To do. That is, the lot determination processing unit 424 executes lot determination by comprehensive determination based on the determination results of the plurality of work lines 80.

  Specifically, the lot determination processing unit 424 executes the lot determination based on the pass / fail determination in the work line 80 having the lot ID of the lot for which the lot determination is to be performed.

  For example, the lot determination processing unit 424 determines that the lot determination of the lot to be determined is acceptable when the line determination is successful in 60% of the work lines 80 having the lot ID, and the three work lines 80 determine the lot to be determined. Assume that a lot ID is included. In this case, when the first work line 80 is “pass”, the second work line 80 is “pass”, and the third work line 80 is “fail”, the lot determination processing unit 424 determines the lot to be determined. It is determined that the lot determination is acceptable.

  When the lot work time is long, the lot determination may be executed by dividing the time every predetermined time (for example, 8 hours or 12 hours). In this case, when the lot determination is performed while the work is suspended, a dummy result may be used, or it may be determined that the work is being suspended.

(Other)
The lot determination processing unit 424 can perform determinations and data analysis other than those described above using the culture medium information, line information, lot information, and work management DB 404, and the determination result or data analysis via the report processing unit 425. The result can be provided to the administrator terminal device 20 so that the administrator can view the result.

  Specifically, the lot determination processing unit 424 checks the reliability of the sample cultured in each film-type medium 60, that is, checks whether or not an inspection error has occurred. The medium information in the medium ID is subjected to a predetermined analysis of the medium information or the medium image data in time series from the culture start time to the end of the culture.

  For example, the lot determination processing unit 424 has a specific culture medium ID (same culture medium ID) 66 based on an instruction from the administrator received via the administrator terminal device 20, and starts from the culture start time to a predetermined time. Are extracted together with the medium image data. Then, the lot determination processing unit 424 performs a predetermined analysis on each medium information and each image data along a predetermined time series, or aggregates each medium information and each image data along a time series. In addition, the lot determination processing unit 424 causes the report processing unit 425 to generate the analysis result or the aggregation result as browsing data having a predetermined data format, and allows the administrator to browse the generated browsing data. 20 to provide.

  Note that the lot determination processing unit 424 is linked to the report processing unit 425, and at this time, based on an instruction from the manager terminal device 20, each medium information in one medium ID 66 is used for legal hygiene management reporting. May be made individually viewable.

  Further, the lot determination processing unit 424 may have a search function for medium information, line information, lot information, or work management information. Specifically, the lot determination processing unit 424 uses the lot ID (instruction ID 71a), the work line ID, the process ID 71b, the work ID 71, the culture start time, the lot work date and time, the culture medium ID 66, the specimen type, and the like as search keys. The database 400 may be searched, and the report processing unit 425 may be linked to generate data that allows the administrator to view the corresponding medium information and various types of information.

  Note that the report processing unit 425 is configured to search for medium information (medium image data, number of colonies and medium determination result), process information, and line specified by a search based on an instruction from the administrator received via the administrator terminal device 20. It is also possible to provide information or information on lots in a predetermined report format. That is, the report processing unit 425 is configured to be able to provide various information as evidence or reports in legal inspection reports and other cases.

[5.5] Report Processing Unit Next, details of the report processing unit 425 in the server device 40 of the present embodiment will be described.

  The report processing unit 425 performs data analysis related to hygiene management in the designated lot, work line 80 or process based on the administrator's instruction input to the administrator terminal device 20 and template data having a predetermined report format. An analysis result (that is, a determination result of pass / fail determination) is generated as report data, and the generated report data is provided to the administrator terminal device 20 so as to be viewable by the administrator via the communication control unit 410.

  Specifically, the report processing unit 425 extracts necessary information from the medium information, the line information, and the lot information such as the medium image data, the number of colonies, or the medium determination result, and the extracted information and the lot determination processing unit 424. Based on the analysis result obtained by the above, report data is generated while assigning each information to the template data. Then, the report processing unit 425 provides the administrator terminal device 20 with report data having an image of a medium in which the processing such as rotation is not executed and the identity is ensured.

  Note that, as described above, the report processing unit 425 is configured to generate predetermined report data or browsing data in accordance with various analyzes or searches and provide the data to the administrator terminal device 20. . In other words, the report processing unit 425 can also provide medium information, process information, line information, or lot-related information specified by the search in a predetermined report format based on the received administrator's instruction. In addition, it is configured so that various information can be provided as evidence or reports in statutory inspection reports and other cases.

[6] Hygiene management system operation process [6.1] Medium information registration process (portable communication terminal device)
Next, operation | movement of the culture medium information registration process in the portable communication terminal device 10 of this embodiment is demonstrated using FIG. In addition, FIG. 12 is a flowchart which shows the registration processing operation | movement of the culture medium information in the portable communication terminal device 10 of this embodiment.

  In this operation, it is assumed that corresponding lot information and line information associated with the work instruction sheet 70 are already stored in the line information DB 402 and the lot information DB 403.

  Further, it is assumed that the medium ID 66 or the instruction ID 71a is attached to the film-type medium 60 and the work instruction sheet 70 by a two-dimensional barcode.

  Further, in this operation, the number of colonies in each film-type medium 60 is detected at the time of registration in the medium information database 400, and the portable communication terminal device 10 uses RGB for capturing a medium image. It is assumed that calibration for the gradation value or the luminance value is performed.

  First, in the portable communication terminal device 10, when the application control unit 120 detects an activation instruction for the culture medium registration application via the operation unit 170 (step S101), the application control unit 120 reads the culture medium registration application from the application storage unit 101 and activates it ( Step S102). At this time, the application control unit 120 performs initialization of the work memory and other necessary processes under the control of the mobile terminal management control unit 190.

  Next, the application control unit 120 interlocks with the display control unit 161 to display an image for selecting whether the initial registration of the film-type medium 60 or the registration after culturing after a predetermined time has elapsed from the start of the culture. It is displayed on the display unit 160 and waits for its input (step S103).

  Next, when detecting an input of a selection instruction from the operation unit 170 (step S104), the application control unit 120 determines whether initial registration or post-cultivation registration and sets the result in flag information (step S105).

  Next, the application control unit 120 causes the display unit 160 to display a screen that prompts the imaging of the film-type medium 60, and waits for imaging by the image data generation unit 110 in conjunction with the operation unit 170 (step S106). At this time, the application control unit 120 causes a predetermined image to be superimposed on the display unit 160 in order to align the medium image to be acquired.

  Next, when the application control unit 120 detects imaging by the image data generation unit 110 in conjunction with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S107), the image data generation unit 110 causes the medium ID 66 to be detected. At the same time, the imaged medium image data of the film type medium 60 is acquired and stored in the image data storage unit 102 while giving a predetermined image ID (step S108).

  Next, the application control unit 120 acquires the current time from the timer 180 as the imaging time of the medium image data, and stores it in the image data storage unit 102 as metadata in association with the medium image data (step S109).

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in a predetermined area of the acquired imaged culture medium image data, acquires the culture medium ID 66, and stores the image data in association with the culture medium image data. The information is stored in the unit 102 (step S110).

  Next, the application control unit 120 causes the display unit 160 to display a screen for acquiring the instruction sheet image data together with the instruction sheet ID 71a and the process ID 71b in conjunction with the display control unit 161, and waits for acquisition of the instruction sheet image data. (Step S111).

  Next, when the application control unit 120 detects imaging by the image data generation unit 110 in cooperation with the image data generation unit 110, the display control unit 161, and the operation unit 170 (step S112), an instruction in which the work ID 71 is imaged. The book image data is acquired and stored in the image data storage unit 102 in association with the culture medium image data acquired in step S108 (step S113).

  In addition, when acquiring the process ID 71b as the work ID 71, or when acquiring the one process ID 71b from the plurality of process IDs 71b, the application control unit 120 operates in conjunction with the display control unit 161 and the operation unit 170, and the user The process ID 71b to be acquired is displayed, and the corresponding process ID 71b is acquired.

  Next, the application control unit 120 analyzes the two-dimensional barcode formed in a predetermined area of the imaged instruction sheet image data and the culture medium image data, acquires the work ID 71, and associates it with the instruction sheet image data. And stored in the image data storage unit 102 (step S114).

  Next, the application control unit 120 acquires the current time as the imaging time of the medium image data from the timer 180, and in conjunction with the display control unit 161 and the operation unit 170, the application control unit 120 acquires the acquired instruction image data, work ID 71, The medium image data, medium ID 66, and imaging time are confirmed (step S115).

  Note that the application control unit 120 may re-acquire the culture image data based on an instruction from the operator when the acquired medium information is confirmed by the operator. At this time, the application control unit 120 may be able to correct the medium image data by the operator in conjunction with the display control unit 161 and the operation unit 170.

  Next, the application control unit 120, based on the operator's instruction, together with flag information indicating whether it is initial registration or post-culture registration, terminal ID, and work ID 71, the acquired medium image data, its metadata, and whether it is initial registration. Flag information indicating whether it is registered after culture is transmitted as medium information to the server device 40 via the network communication unit 130 (step S116), and the registration process of the portable communication terminal device 10 is terminated.

  At this time, when transmitting the culture medium information, the network communication unit 130 executes login for accessing the server device 40 based on the terminal ID or the input ID and password, and the server device 40 is completed after the login is completed. Communication line is established and medium information is transmitted.

[6.2] Medium information registration processing (server device)
Next, the medium information registration processing operation in the server device 40 of the present embodiment will be described with reference to FIG. FIG. 13 is a flowchart showing the operation of registering medium information in the server device 40 of the present embodiment.

  In addition, this operation | movement is a process performed in conjunction with the culture medium information registration process performed by the portable communication terminal device 10, and the server apparatus 40 is based on the culture medium information transmitted from the portable communication terminal device 10. The following processing is executed.

  First, in the server device 40, when the communication control unit 410 receives the culture medium information transmitted from the portable communication terminal device 10 (step S300), the registration processing unit 421 is based on the flag information included in the received culture medium information. Whether the registration is initial registration or post-culture registration is determined (step S301).

  At this time, if the registration processing unit 421 determines initial registration, the process proceeds to step S302. If the registration processing unit 421 determines that it is not initial registration, that is, if it is determined to be post-culture registration, step S304 is performed. Move on to processing.

  Next, when it is determined as initial registration, the registration processing unit 421 extracts and specifies the work ID 71 included in the culture medium information (step S302).

  Next, the registration processing unit 421 registers the culture medium information in the culture medium information DB 401 based on the identified work ID 71 (step S303), and ends this operation. Although the culture start time is stored in advance in the work management DB 404, the registration processing unit 421 registers the imaging time included in the received medium information as the culture start time in the work management DB 404 in the process of step S303. Also good.

  On the other hand, if the registration processing unit 421 determines that the registration is after culture, it extracts and specifies the work ID 71 included in the culture medium information (step S304), and registers the culture medium information based on the imaging time included in the culture medium information. The culture examination time to be specified is specified (step S305).

  At this time, the registration processing unit 421 may be registered by designating a culture examination time for registering the culture medium information in conjunction with the portable communication terminal device 10, or the imaging time included in the received culture medium information. The culture examination time may be registered as it is. In addition, the registration processing unit 421 manages the culture medium registration at the culture test time that has already been performed, and specifies the culture test time based on the timing at which the culture medium information is received and the culture test time that has already been used to register the culture medium information. May be. That is, the registration processing unit 421 may specify the culture examination time by rewriting the execution flag each time the medium information is registered and comparing the received imaging time with reference to the information of the flag.

  Next, the registration processing unit 421 causes the colony detection determination unit 422 to perform image analysis on the received medium image data, and the feature amount (luminance value) of each pixel constituting the region of the culture layer 63 in the medium image. And the number of colonies generated in the specimen imaged based on the feature quantity of each pixel constituting the region of the culture layer 63 is identified and counted (step S307).

  Details of the colony identification and counting process in the process of step S307 will be described later.

  Next, the colony detection determination unit 422 reads out the medium determination criterion based on the type of the sample specified based on the work ID 71 and the fungus species to be detected, and detects the number of colonies detected based on the read sample determination criterion. It is determined whether or not satisfies a predetermined condition (step S308). That is, the colony detection determination unit 422 determines that it is acceptable (normal) when the predetermined condition is satisfied, and determines that it is unacceptable (abnormal) when the predetermined condition is not satisfied.

  The colony detection determination unit 422 is linked to the portable communication terminal device 10 instead of the automatic registration described above, and displays the detected number of colonies and the result of the pass / fail determination on the display unit 160 of the portable communication terminal device 10. The user may be allowed to view and confirm.

  Next, the registration processing unit 421 specifies culture start time information using the culture image data, the recognized medium ID 66, the determined pass / fail, the number of detected colonies, and the imaging time included in the medium information as the culture start time. In association with the work ID 71, it is registered in the culture medium information DB 401 (step S309), and this operation is terminated.

  The registration processing unit 421 displays line information and information to be registered on the display unit 160 of the portable communication terminal device 10, and based on a user instruction input via the portable communication terminal device 10, the culture medium Information or the like may be registered.

  Further, when the culture medium image data is transmitted from the portable communication terminal apparatus 10 and the medium ID 66 is recognized by analyzing each image data by the server apparatus 40, the registration processing unit 421 receives the reception in the process of step S301. The medium ID 66 imaged on each predetermined area of the medium image data thus obtained is analyzed, and the medium ID 66 is recognized and acquired. Similarly, the server device 40 also recognizes the work ID 71 in the instruction image data.

[6.3] Colony Detection Process Next, the operation of the colony detection process in the server device 40 of the present embodiment will be described with reference to FIGS. 14 and 15. 14 is a flowchart showing the operation of the colony detection process in the server device 40 of the present embodiment, and FIG. 15 is an image of the color feature amount of the non-colony pixel specified in the culture medium image in which the colony is to be detected. FIG.

  This operation is a process executed in the colony detection determination unit 422 under the control of the registration processing unit 421 in the process of step S307 of the culture medium registration process shown in FIG.

  First, the colony detection determination unit 422 executes a color feature amount extraction process for extracting a color feature amount (for example, a luminance value) of each pixel constituting the region of the culture layer 63 in the culture medium image acquired from the culture image data. (Step S401).

  Next, the colony detection determination unit 422 counts the number of pixels for each extracted color feature amount, and counts the number of pixels to generate a histogram (probability distribution of color feature amount) as the number of pixels for each color feature amount. (Step S402).

  Next, the colony detection determination unit 422 specifies a non-colony pixel based on the specified color feature amount while specifying a color feature amount satisfying a predetermined condition based on the generated histogram (step S403).

  Next, the colony detection determination unit 422 executes a non-colony pixel feature amount specifying process for specifying a feature amount of a representative non-colony pixel from the plurality of specified non-colony pixels (step S404).

  FIG. 15 shows a medium image for image analysis and the color feature amount of the non-colony pixel detected from the medium image by executing the non-colony pixel feature amount specifying process as described above.

  As shown in FIG. 15A, a medium image in which many colonies appear (colony appearance pattern 1), and as shown in FIG. 15B, a medium in which colonies appear sparsely is displayed as an image (colony colony). As shown in the appearance pattern 2) and FIG. 15C, in the case of a medium image in which no colonies appear, the color characteristics of the non-colony pixels are superimposed on the right corner of each medium image. Specify the amount.

  Next, the colony detection determination unit 422 executes colony pixel detection processing for detecting, as a colony pixel, a pixel having a color feature amount having a predetermined color difference from the color feature amount of a non-colony pixel (step S405).

  Next, the colony detection determination unit 422 executes colony detection processing for counting the number of colonies while detecting colonies based on the detected colony pixels (step S406), and ends the operation.

[7] Modification [7.1] Modification 1
In the above-described embodiment, the culture medium information registration process and the colony detection determination process are executed at the same timing, but the colony detection determination process may be executed at an arbitrary timing.

[7.2] Modification 2
In this embodiment, instead of performing the culture information registration process using a portable communication terminal device 10 such as a tablet information terminal device, a smartphone, or a mobile phone, a laptop or desktop personal computer is used. The registration process of the culture medium information may be realized by a computer and an image input device such as a scanner, a digital camera, or a smartphone.

  In this case, the personal computer and the image input device may be connected according to a predetermined communication standard, and the registration process of the culture medium information may be realized by using the personal computer and the image input device integrally, or a memory card. The medium image data and instruction sheet image data previously acquired by the image input device are stored in other physical memory, and the registration process of the medium information is realized while the stored image data is captured by the personal computer. May be.

[7.3] Modification 3
In the present embodiment, the portable communication terminal device 10, the administrator terminal device 20, and the server device 40 may be installed or used in the same site, or each may be installed in a remote place such as outside the country or remotely. It may be used on the ground and each process described above may be executed. However, it is assumed that the portable communication terminal device 10 is used in the same lot.

[7.4] Modification 4
In the present embodiment, registration of medium information in a specimen cultured in the film-type medium 60 using the film-type medium 60 is executed, but the medium in the specimen cultured in the agar medium using an agar medium. Information registration may be executed.

[7.5] Modification 5
In the present embodiment, instead of acquiring the culture medium ID 66 by imaging the film-type culture medium 60 by the image data generation unit 110, the culture medium ID 66 may be input by an operator's operation input.

  In this case, the application control unit 120 interlocks with the display control unit 161 and the operation unit 170 and causes the operator to manually input the culture medium ID 66 directly using the operation unit 170.

  In addition, the application control unit 120 controls the display control unit 161 and the operation unit 170 to select a display item such as a pull-down menu by clicking or touch selection, and causes the operator to input the culture medium ID 66.

[7.6] Modification 6
In the present embodiment, various processes are executed by the single server device 40, but various processes may be executed by a server system including a plurality of server devices 40.

  As described above, in the sanitary management system S of the present embodiment, the color feature amount of the pixel where the colony is not formed for each film type medium 60 (that is, the color feature amount of the medium itself) is specified, and the medium itself is identified. Since the colony pixels constituting the colony can be specified based on the feature amount of the color of the pixel, the individual difference of the color of the film type medium 60 in which the colony is cultured, the imaging environment when the film type medium 60 is imaged Even if the color of the film-type medium 60 varies, such as when the color of the film-type medium 60 after the colony culture and the color of the film-type medium 60 varies depending on the state of colony generation, colony pixels are detected according to the variation can do.

  Therefore, in the sanitary management system S of the present embodiment, colonies can be accurately detected in any situation, and medium information including information on the colonies that have been accurately detected can be registered in the database.

  Further, in the sanitary management system S of the present embodiment, it is possible to save resources as compared with the case where various data analyzes and provisions are performed using physical resources such as paper media, and the culture medium. By registering the culture medium information in the database, registration mistakes can be remarkably reduced and difficult medium management can be facilitated, so that resource saving can be achieved without wastefully consuming the culture medium.

  In the sanitary management system S of the present embodiment, the description has been made using food. However, the present invention is not limited to the food, and can be applied to products that are provided to all living organisms including drugs and drugs. .

  The present invention can be used for food and other sanitation management systems.

DESCRIPTION OF SYMBOLS S ... Hygiene management system 10 ... Portable communication terminal device 20 ... Manager terminal device 30 ... Network 40 ... Server device 60 ... Film type culture medium 70 ... Work instruction sheet 80 ... Work line 100 ... Data storage part 101 ... Application storage part 102 Image data storage unit 110 Image data generation unit 120 Application control unit 130 Network communication unit 140 Short-range wireless communication interface 150 Current position detection unit 160 Display unit 170 Operation unit 180 Timer 190 Mobile device management Control unit 400 ... database 401 ... medium information DB
402 ... Line information DB
403 ... Lot information DB
404 ... Work management DB
410 ... Communication control unit 420 ... Data processing unit 421 ... Registration processing unit 422 ... Colony detection determination unit 424 ... Lot determination processing unit 425 ... Report processing unit 430 ... Server management control unit

Claims (8)

An acquisition means for acquiring, as medium image data, data of a medium image obtained by imaging and imaging a medium for culturing colonies;
Extraction means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the culture medium image of the acquired culture medium image data;
Based on the extracted color feature amount, a non-colony pixel that is a pixel of the medium itself is specified, and a specifying unit that specifies a color feature amount of the non-colony pixel from the specified non-colony pixel;
Detecting means for detecting, as a colony pixel, a pixel having a color feature amount having a predetermined color difference from the color feature amount of the specified non-colony pixel, and detecting the colony based on the detected colony pixel;
Registration means for registering information about the colonies in the database as medium information, including the detected colonies;
Equipped with a,
The specifying unit counts the number of pixels for each color feature amount, and accumulates the number of pixels of each color feature amount in order from the color feature amount indicating the color of the culture medium to the color feature amount indicating the color of the colony. A color feature amount when the number of pixels obtained becomes a value corresponding to a predetermined ratio of the total number of pixels constituting the predetermined region is specified as a reference feature amount, and the reference feature amount is determined from the color feature amount indicating the color of the culture medium The culture medium information registration system characterized by specifying the pixel which belongs to the range to a non-colony pixel . The culture medium information registration system according to claim 1, wherein the predetermined ratio is ½. In the culture medium information registration system according to claim 1 or 2 ,
The color feature amount is a color feature amount of two or more colors;
The culture medium information registration system, wherein the color difference is defined by the similarity of histograms of two or more color feature values. In the culture medium information registration system according to any one of claims 1 to 3 ,
The culture medium information registration system, wherein the color difference is a difference in Euclidean distance based on the color feature amount. Computer
An acquisition means for acquiring, as medium image data, data of a medium image that is imaged by imaging a medium for culturing a colony,
Extraction means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the medium image of the acquired medium image data;
A specifying means for specifying a non-colony pixel, which is a pixel of the medium itself, based on the extracted color feature amount, and specifying a color feature amount of the non-colony pixel from the specified non-colony pixel;
Detecting means for detecting, as a colony pixel, a pixel having a color feature amount having a predetermined color difference from the color feature amount of the specified non-colony pixel, and detecting the colony based on the detected colony pixel; and
Registration means for registering information about the colonies in the database as medium information, including the detected colonies;
To function as,
The specifying unit counts the number of pixels for each color feature amount, and accumulates the number of pixels of each color feature amount in order from the color feature amount indicating the color of the culture medium to the color feature amount indicating the color of the colony. A color feature amount when the number of pixels obtained becomes a value corresponding to a predetermined ratio of the total number of pixels constituting the predetermined region is specified as a reference feature amount, and the reference feature amount is determined from the color feature amount indicating the color of the culture medium A program characterized by specifying pixels belonging to the above range as non-colony pixels . An acquisition means for acquiring, as medium image data, data of a medium image obtained by imaging and imaging a medium for culturing colonies;
Extraction means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the culture medium image of the acquired culture medium image data;
Based on the extracted color feature amount, a non-colony pixel that is a pixel of the medium itself is specified, and a specifying unit that specifies a color feature amount of the non-colony pixel from the specified non-colony pixel;
Detecting means for detecting, as a colony pixel, a pixel having a color feature amount having a predetermined color difference from the color feature amount of the specified non-colony pixel, and detecting the colony based on the detected colony pixel;
Equipped with a,
The specifying unit counts the number of pixels for each color feature amount, and accumulates the number of pixels of each color feature amount in order from the color feature amount indicating the color of the culture medium to the color feature amount indicating the color of the colony. A color feature amount when the number of pixels obtained becomes a value corresponding to a predetermined ratio of the total number of pixels constituting the predetermined region is specified as a reference feature amount, and the reference feature amount is determined from the color feature amount indicating the color of the culture medium A colony detection device that identifies pixels belonging to the above range as non-colony pixels . Computer
An acquisition means for acquiring, as medium image data, data of a medium image that is imaged by imaging a medium for culturing a colony,
Extraction means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the medium image of the acquired medium image data;
Based on the extracted color feature amount, a non-colony pixel that is a pixel of the culture medium itself is specified, and a specifying unit that specifies a color feature amount of the non-colony pixel from the specified non-colony pixel, and
Detecting means for detecting a pixel having a color feature amount having a predetermined color difference from the color feature amount of the identified non-colony pixel as a colony pixel, and detecting the colony based on the detected colony pixel;
Function as
The specifying unit counts the number of pixels for each color feature amount, and accumulates the number of pixels of each color feature amount in order from the color feature amount indicating the color of the culture medium to the color feature amount indicating the color of the colony. A color feature amount when the number of pixels obtained becomes a value corresponding to a predetermined ratio of the total number of pixels constituting the predetermined region is specified as a reference feature amount, and the reference feature amount is determined from the color feature amount indicating the color of the culture medium program and identifies the pixels belonging to a range of up to a non-colony pixels. A communication terminal device that generates a medium image obtained by imaging a medium for cultivating bacteria generated in a specimen as medium image data;
A server device for detecting colonies generated in the medium based on the medium image data and registering them in a database;
With
The server device is
An acquisition means for acquiring the culture medium image data from the communication terminal device;
Extraction means for extracting a color feature amount relating to a color of a pixel constituting a predetermined region in the culture medium image of the acquired culture medium image data;
Based on the extracted color feature amount, a non-colony pixel that is a pixel of the medium itself is specified, and a specifying unit that specifies a color feature amount of the non-colony pixel from the specified non-colony pixel;
Detecting means for detecting, as a colony pixel, a pixel having a color feature amount having a predetermined color difference from the color feature amount of the specified non-colony pixel, and detecting the colony based on the detected colony pixel;
Registration means for registering information about the colonies in the database as medium information, including the detected colonies;
With
The specifying unit counts the number of pixels for each color feature amount, and accumulates the number of pixels of each color feature amount in order from the color feature amount indicating the color of the culture medium to the color feature amount indicating the color of the colony. A color feature amount when the number of pixels obtained becomes a value corresponding to a predetermined ratio of the total number of pixels constituting the predetermined region is specified as a reference feature amount, and the reference feature amount is determined from the color feature amount indicating the color of the culture medium A hygiene management system characterized by identifying pixels belonging to the above range as non-colony pixels .

Images