JP6499740B2 - Food composition - Google Patents

Description

  The present invention relates to a food composition.

  Conventionally, various food compositions for adjusting the physical condition have been proposed. Among these, food compositions containing specific fungi or fungal extracts are said to be effective in promoting health.

  For example, among fungi, beef turf is effective in normalizing immune functions and is also used as a medicine.

  However, it is also known that pharmaceuticals or food compositions containing only the extract of Benicus mushroom are limited in terms of effects.

  In this regard, an immunomodulating preparation containing lactic acid bacteria in addition to Benix nokitake has been proposed (for example, Patent Document 1).

  However, there are many types of lactic acid bacteria, some of which contribute to health and some that do not. Therefore, it cannot be said that any kind of lactic acid bacteria enhances the immunoregulatory function of the extract of Benicus mushroom.

Utility Model Registration No. 3159091

  The problem to be solved by the present invention is to provide a food composition containing Benicus mushroom, which further enhances the function of normalizing immune function.

  The present invention provides a food composition comprising Benicus mushroom and Narine fungus.

  ADVANTAGE OF THE INVENTION According to this invention, it is a food composition containing Benicus mushroom, Comprising: The food composition which further improved the function which normalizes an immune function can be provided.

The figure which shows the change of the bodily sensation according to the past disease in the whole test subject in the 1st period survey and the 2nd period survey. The figure which shows the example of the answer column of VAS. The figure which shows the result of a 1st investigation. The figure which shows the result of a 2nd investigation. The figure which shows the result of a 3rd investigation. The figure which shows the change of the bodily sensation according to the past symptoms in the whole test subject. The figure which shows the 1st remarkable example. The figure which shows the 2nd remarkable example. The figure which shows the breakdown of the subject's past illness. The figure which shows the average value of the reply result of VAS regarding the bodily sensation of "fatigue", "pain", and "tone". The figure which shows the total result of the improved bodily sensation. The figure which shows the ratio with respect to the whole test subject of the test subject who felt the presence or absence of the improved bodily sensation. The figure which shows IL-12 productivity of a macrophage by a test substance alone. The figure which shows the induction | guidance | derivation by CpG-DNA of a macrophage. The figure which shows the result of IL-12 induction | guidance | derivation by the combination of a Benicus nokitake extract and Narine bacteria.

  Hereinafter, the food composition concerning one embodiment of the present invention is explained, referring to drawings.

(First embodiment)
The food composition according to the present embodiment includes Benixonochatake (Antrodia Cinnamomea) and Narine fungus (Lactobacillus acidophilus Er317 / 402). This food composition contributes to normalization of immune function.

(Benix nokitake)
Benix nokitake is a fungus that is unique to Taiwan, and only inhabits the persimmon trees that are found in Taiwan.

  The mycelium of the solid culture of Benix nokitake includes anthroquinonol, polysaccharides, triterpenes, superoxide dismutase, nucleic acids, GABA (γ-aminobutyric acid) and the like.

(Antroquinonol)
Antroquinonol has the following structural formula:

  Antroquinonol is a cyclohexane ketone compound having a linear hydrocarbon. Antroquinonol is anti-tumor (cancer cell autophagy, apoptosis induction), immune regulation, inflammation suppression, hypercholesterolemia, arteriosclerosis, fatty liver, liver fibrosis improvement, kidney protection, skin condition It is known that there is an effect such as improvement.

  The antitumor action of antroquinonol is considered as follows. EGF (epidermal growth factor) will be described as an example. When the GDP of the RAS protein that has been inactivated by binding to GDP is replaced by GTP, the RAS protein is activated and the cells proliferate. In normal cells, the activated RAS protein GTP is dephosphorylated to GDP, the RAS protein is inactivated, and cell growth stops.

  If the RAS protein is abnormal, the activated RAS protein GTP does not change to GDP, and the cells continue to grow.

  Antroquinonol is thought to inhibit FTase, which activates RAS protein. If the RAS protein cannot be activated, the cells die by autophagy. Therefore, antroquinonol has a function to stop the disordered growth of cells.

  Other effects of antroquinonol include LDL cholesterol reduction, C-reactive protein reduction, arteriosclerosis improvement, inhibition of NF-kB activity, increase of Nrf2 activity, reduction of urine protein, reduction of hematuria, reduction of BUN and creatinine. It is known that neutrophil accumulation in the kidney is reduced, creatinine kinase is inhibited, and lactate dehydrogenase is inhibited.

(Polysaccharide)
Benixotake contains, for example, β-D glucan as a polysaccharide. β-D glucan is said to be effective in improving immune function, antitumor, suppressing high blood pressure, reducing cholesterol, and improving symptoms of diabetes.

(Triterpene)
Benix nokitake is a triterpene, such as anthosine A, anthosine B, anthosine C, anthosine E, anthosine F, zancic acid D, zancic acid E, 15α-acetyl dehydrosulfuric acid, dehydroebric acid, dehydrosulfuric acid, etc. Including about 200 types. These triterpenes are said to be effective for liver protection, liver detoxification function improvement and liver cell regeneration, improvement of hypertension, suppression of inflammation, immune regulation and the like.

(Superoxide dismutase)
Benix nokitake contains superoxide dismutase. This superoxide dismutase is said to be effective in suppressing toxicity of active oxygen, antioxidant, anti-aging, and prevention of lifestyle-related diseases.

(Nucleic acid)
Benixotake contains adenosine as a nucleic acid. Adenosine is said to be effective in hair-growth effect, blood circulation promotion effect, prevention of heart disease and brain disease.

(GABA)
GABA is said to have effects such as a liver function improving action and a blood pressure rise inhibiting action.

(Narine fungus)
Narine bacteria are a type of lactic acid bacteria. Narine bacteria are derived from newborns and are known to have antibacterial and acid-resistant effects.

  Narine bacteria suppress the growth of harmful bacteria and contribute to the improvement of the intestinal environment.

(Other ingredients)
The food composition of the present embodiment may contain other components. Examples of other components may include polyphenols, vitamins, minerals, and other additives. Even if these components are contained, the normalizing action of immune function is not inhibited.

  Examples of polyphenols include hesperidin, vitamins include vitamin B2 and vitamin B12, and minerals include calcium, magnesium, zinc, iron, and molybdenum.

  In particular, since hesperidin can be expected to contribute to normalization of immune function, the effect of this embodiment is expected to be maintained or further enhanced.

  Other additives and surfactants, excipients, saccharides and the like can be mentioned.

(Example)
The food composition of this embodiment has a daily intake of 250 mg to 500 mg when the content of anthroquinonol is 5 mg / g or more in Benix nokitake powder, and Narine has a bacterial density of 1 billion. / G is adjusted to be 100 mg or more and 10 g or less. Among these, the most desirable blending weight ratio is Benixonocha mushroom powder: Narine bacteria = 250 mg: 150 mg = 5: 3 in the case of the above-described content rate. In each of the following prescription examples, the food composition is adjusted so that the powder of Benixonocha mushroom: Narine bacteria = 250 mg: 150 mg.

  If the daily intake is below each of the above minimum values, the effect of normalizing immune function is poor, and even if the daily intake exceeds the above maximum values, it is expected that the effect will be proportional to the intake Can not.

(Prescription Example 1)
Benix nokitake extract powder 15.200% by weight
Narine powder 9.100% by weight
Maltitol 44.700% by weight
Crystalline cellulose 15.000% by weight
Sucrose fatty acid ester 4,000% by weight
Seaweed powder 10.000% by weight
Silicon dioxide 2,000% by weight

  Here, Benix nokitake extract powder was manufactured by Golden Biotechnology Corporation. This Benix nokitake extract powder contains 5 mg / g or more of antroquinonol. Moreover, Narine bacteria used VITAWAY of Armenia. The density of Narine bacteria is 1 billion cells / g. The same applies to the following prescription examples.

(Prescription example 2)
Benix nokitake extract powder 15.200% by weight
Narine powder 9.100% by weight
Hesperidin 15.200% by weight
Maltitol 34.500% by weight
Crystalline cellulose 10.000% by weight
Sucrose fatty acid ester 4,000% by weight
Seaweed powder 10.000% by weight
Silicon dioxide 2,000% by weight

(Production method)
The ingredients of each of the above formulations are stirred with a mixer to produce a preliminary granule, and a binder is added to the preliminary granule for tableting. The tablet that was tableted was coated, and 330 mg was taken as one tablet to make the final tablet.

(Experiment 1)
Forty-three subjects with a history were divided into 21 members in the first group and 22 members in the second group. In the first phase survey, the food composition of Formulation Example 1 was taken once a day for 5 consecutive tablets (corresponding to 250 mg of Benix nokitake, 150 mg of Narine bacteria) for the first group for 30 consecutive days. . Thereafter, 30 days later, the food composition of Formulation Example 2 was taken once a day for 5 consecutive days (equivalent to 250 mg of Benix nokitake, 150 mg of Narine fungus, 250 mg of hesperidin) for 30 consecutive days.

  In the second phase survey, the food composition of Formulation Example 1 was taken once a day for 5 consecutive tablets (corresponding to 250 mg of Benix nokitake, 150 mg of Narine) for the second group for 30 consecutive days. . Thereafter, 30 days later, the food composition of Formulation Example 2 was taken once a day for 5 consecutive days (equivalent to 250 mg of Benix nokitake, 150 mg of Narine fungus, 250 mg of hesperidin) for 30 consecutive days.

(Result of Experiment 1)
Since there was no significant difference between the results of Formulation Example 1 and Formulation Example 2, only the results of Formulation Example 1 are shown.

  FIG. 1 is a diagram showing changes in body sensation according to previous symptoms in all subjects in the first and second phase studies.

  As shown in FIG. 1, 13 out of 20 subjects suffering from cancer and 8 out of 12 subjects suffering from rheumatism felt that the feeling was improved. Overall, 30 out of 43 subjects, or 69.8%, felt that the experience was improved.

  For further investigation, fatigue, pain, and physical condition were evaluated by VAS (visual analog scale).

  FIG. 2 is a diagram illustrating an example of a VAS answer column. As shown in FIG. 2, the better physical condition was set to “1”, the worse physical condition was set to “9”, and the subject's sensation was evaluated before ingestion and after ingestion for 30 days.

  FIG. 3 is a diagram showing the results of the first period survey. FIG. 3 shows the average value of VAS answer results for 8 subjects suffering from cancer. As shown in FIG. 3, 75% of the subjects felt that the sensation was improved. In particular, “fatigue” was markedly improved.

  FIG. 4 is a diagram showing the results of the second period survey. FIG. 4 shows the average value of VAS answer results for 12 subjects suffering from cancer. As shown in FIG. 4, 67% of the subjects felt that the sensation was improved. In particular, “fatigue” was markedly improved.

  FIG. 5 is a diagram showing the results of the first period survey and the second period survey. FIG. 5 shows the average value of the VAS answer results for 12 subjects suffering from rheumatism. As shown in FIG. 5, 67% of the subjects felt that the sensation was improved. Significant improvement was observed for any of “fatigue”, “pain”, and “physical condition”.

In each survey, the following differences in experience were described in the free description column.
・ The constipation was cured.
・ Spleen cancer pain decreased and basal body temperature increased.
・ Spleen cancer has become smaller and no surgery is required.
・ The efficacy of anticancer drugs has improved.
・ Improved stool condition and became able to sleep.
・ The number of lymphocytes and white blood cells increased.
・ Lung cancer improved.
・ Decreased white blood cells caused by administration of anticancer drugs.
・ Appetite has returned.

  As described above, with regard to Formulation Example 1 and Formulation Example 2, improvement in body sensation was observed particularly for subjects with a history of immune system diseases. Therefore, the food composition of this embodiment has an effect of contributing to normalization of immune function.

(Second Embodiment)
The food composition according to the present embodiment includes Benixonokitake (Antrodia Cinnamomea), Narine fungus (Lactobacillus acidophilus Er317 / 402), and broccoli. This food composition further contributes to normalization of immune function.

(broccoli)
Broccoli (Brassica oleracea var. Italica) is a kind of green-yellow vegetable of the genus Brassicaceae that is usually available in the city. Broccoli is rich in immunologically active substances.

  Therefore, normalization of immune function can be further promoted by adding broccoli to the formulation.

(Example)
The food composition of this embodiment has a daily intake of 250 mg to 500 mg when the content of anthroquinonol is 5 mg / g or more in Benix nokitake powder, and Narine has a bacterial density of 1 billion. / G is adjusted to be 100 mg or more and 10 g or less. Among these, the most desirable blending weight ratio is Benixonocha mushroom powder: Narine bacteria = 250 mg: 150 mg = 5: 3 in the case of the above-described content rate. In each of the following prescription examples, the food composition is adjusted so that the powder of Benixonocha mushroom: Narine bacteria = 250 mg: 150 mg.

  If the daily intake is below each of the above minimum values, the effect of normalizing immune function is poor, and even if the daily intake exceeds the above maximum values, it is expected that the effect will be proportional to the intake Can not.

  The broccoli powder is desirably 50 mg or more and 200 mg or less with respect to the above composition range. Below this range, the effect is poor, and above this range, the size of the tablet increases, making it difficult to drink.

(Prescription Example 3)
Benix nokitake extract powder 15.200% by weight
Narine powder 9.100% by weight
Broccoli powder 3.100% by weight
Seaweed powder 4.000% by weight
Maltitol 51.253 wt%
Crystalline cellulose 12.000% by weight
Sucrose fatty acid ester 4,000% by weight
Vitamin B2 0.104% by weight
Vitamin B12 0.243 wt%
Shellac 0.500% by weight
Carnauba wax

  Here, broccoli powder is broccoli powdered by freeze-drying. In Formulation Example 4, the same broccoli powder was used.

(Prescription Example 4)
Benix nokitake extract powder 15.200% by weight
Narine powder 9.100% by weight
Broccoli powder 3.100% by weight
Hesperidin 15.200% by weight
Seaweed powder 4.000% by weight
Maltitol 36.053 wt%
Crystalline cellulose 12.000% by weight
Sucrose fatty acid ester 4,000% by weight
Vitamin B2 0.104% by weight
Vitamin B12 0.243 wt%
Shellac 0.500% by weight
Carnauba wax

(Production method)
The ingredients of each of the above formulations are stirred with a mixer to produce a preliminary granule, and a binder is added to the preliminary granule for tableting. The tablet that was tableted was coated, and 330 mg was taken as one tablet to make the final tablet.

(Experiment 2)
Forty-seven subjects with a history of disease, the food composition of Formulation Example 3 was taken once a day for 5 consecutive days (equivalent to 250 mg of Benix nocturnus, 150 mg of Narine fungus, 50 mg of broccoli powder) for 30 consecutive days. . Then, 30 days later, the food composition of Formulation Example 4 was taken once a day for 30 consecutive days (equivalent to Benix nokitake 250 mg, Narine fungus 150 mg, broccoli powder 50 mg, hesperidin 250 mg) once a day for 30 consecutive days. went.

(Result of Experiment 2)
Since there was no significant difference between the results of Formulation Example 3 and Formulation Example 4, only the results of Formulation Example 3 are shown.

  FIG. 6 is a diagram showing changes in body sensation according to previous symptoms in the entire subject. As shown in FIG. 6, 24 of 30 subjects suffering from cancer felt that all of the 2 subjects suffering from Sjogren's syndrome had a better feeling. Overall, 33 out of 47 subjects, or 70.2%, felt that the experience was improved.

  FIG. 7 is a diagram showing a first prominent example. As shown in FIG. 7, before ingestion (A), the hair was thin and white was conspicuous, whereas after ingestion (B) for 30 days, black hair was growing.

  FIG. 8 is a diagram showing a second prominent example. As shown in FIG. 8, the inflammation of atopic dermatitis spread on the back before ingestion (A), whereas the inflammation almost disappeared after 30 days of ingestion.

The following were reported as other prominent examples.
・ Pancreatic cancer disappeared and no longer needed surgery.
-Cancer that had metastasized disappeared.
・ No melena due to colorectal cancer.
・ The pain of prostate cancer disappeared.
・ Rheumatic pain disappeared.
・ In multiple sclerosis, improved from being unable to walk to being able to climb stairs.
・ In lupus erythematosus, body temperature rose, white blood cell count, liver function, etc. became normal.
・ Recovery period from the decrease of leukocytes by administration of anticancer drugs was significantly shortened.

(Experiment 3)
Formulation Example 3 and Formulation 4 food composition once per 819 subjects with pre-existing conditions (5 venix nosych mushroom 250 mg, Narine fungus 150 mg, broccoli powder 50 mg, formulation example 4 equivalent to Hesperidin 150 mg), 1 day A single ingestion was conducted for 3 months and a questionnaire was taken. The number of people who returned the questionnaire was 554. There was no significant difference in results between Formulation Example 3 and Formulation Example 4. Accordingly, only the results of Formulation Example 4 are shown below.

  FIG. 9 is a diagram showing a breakdown of the history of the subject. As shown in FIG. 9, more than half of the subjects had a history of cancer, and about 10% of the subjects had a history of rheumatism or autoimmune disease.

(Result of Experiment 3)
FIG. 10 is a diagram illustrating an average value of VAS answer results regarding the bodily sensations of “fatigue”, “pain”, and “tone”. At the beginning and end of drinking every month, a VAS questionnaire was conducted.

  As shown in FIG. 10, in all periods, the end of drinking was improved more than the beginning of drinking for all the feelings of “fatigue”, “pain”, and “tone”.

  Specifically, with regard to “fatigue”, the start of the experiment was 5.35, but was improved to 3.26 at the end of the experiment. Regarding "pain", the start of the experiment was 3.97, but it was improved to 2.88 at the end of the experiment. “Physical condition” was 5.20 at the start of the experiment, but improved to 3.24 at the end of the experiment.

  FIG. 11 is a diagram showing the total results of improved experience. In the questionnaire, subjects were asked to check each experience improved by multiple responses.

  As shown in FIG. 11, in any period of 3 months, “physical improvement”, “test result improvement”, “improvement of fatigue”, “improvement of bowel movement”, “improvement of pain”, “improvement of sleep” ”And other prominent cases were reported, such as“ The cancer disappeared or became smaller ”.

  FIG. 12 is a diagram showing a ratio of subjects who felt the presence or absence of an improved body sensation to the whole subject.

  As shown in FIG. 12, 72% of the subjects felt an improvement in body sensation.

  The following is an excerpt of the items listed in the free entry column of the questionnaire. “Ellen” described below refers to a food composition according to Formulation Example 4.

(cancer)
“My son is so cute that it doesn't hurt if I put it in myself. His son is pancreatic cancer at the end of the stage (Stage 5). The cancer was found to have disappeared when I saw it under a microscope. I saved my life and couldn't find a word of thanks. "

  "Stage 4's stomach, lymph, and aortic cancer have been reduced in size from 1.4cm to 0.9cm, and the cancer of the other side has disappeared. I didn't take any medicine, so I think it was thanks to Ellen.

  "I feel better. I have back pain and back pain. I have improved liver function. There are very few side effects. I was told that I will continue to lose my hair. It was said that the life expectancy was 3 months, and the stomach was about 8 months pregnant with ascites, but there was no ascites storage, good peritoneal dissemination, and no metastasis. "

  “It became malignant lymphoma, and the value of white blood cells was 1900-3300 (reference value 3500) for 2 years and 5 months, but it exceeded the reference value at the end of August and reached 3600. And in the examination on October 31, 4300 "

  “The cancer marker number dropped and the teacher was surprised. I'm so happy that I just don't have to hit anticancer drugs.”

  “The water around the lungs disappeared and the doctor was surprised. The cancer around the throat is getting better little by little.

  “As of July, the number of urinations was 18 times a day and 10 stools. The amount of urination was small and the number of urinations was small, but it was normal on the third day after drinking Ellen. The number of toilets per day has decreased to 1/3, the standard of living has risen, and the cancer marker has become normal. "

  “The body is warm and the feet are stiff. The feet are light. The appetite has also come out. I'm looking forward to work. PSA drastically decreased from 194.8 to 11.4.

  “No cancer metastasis, no recurrence, no radiation treatment. There seems to be no person with breast cancer that has not undergone radiation treatment so far, and doctors said that if there is an opportunity to present at a conference, etc., please let me know ,Was glad."

  "Declared life expectancy as ovarian cancer in 2015. It has spread to all organs and if it cannot be operated, it is said that it has no life until the end of the year. Cancer was found in the lymph and wanted to live for the family and wanted to monitor As a result, the cancer of 8 mm to 1 cm on the bladder disappeared, the tumor on the vagina disappeared, the peritoneal dissemination of the large intestine disappeared, and the tumor of liver metastasis disappeared. Sexual ovarian cancer is getting better, but the original ovarian cancer marker is going up. The body is really subtle. I want to fight Ellen. "

  “I wanted to monitor my mother. On April 8, I had a diagnosis of colorectal cancer (Stage IV) and confirmed metastasis to the liver and lymph. As a result of monitoring (referred to this experiment 3, the same applies hereinafter), small cancer disappeared. The big cancer started to get smaller, and until my doctor told me to do an operation to remove the cancer, my mother, who was said to have a two-year survival rate of 16%, didn't expect it to be so good.

  “I was suffering from nausea, loss of appetite and fatigue after anti-cancer drugs. I participated in the Kakuren monitor since February 2016, but when I was monitoring, the side effects were lightest and my tumor marker. It was the lowest so far.I wanted to continue drinking and I applied.As a result, blood pressure was normal, body temperature kept at 36 degrees, stool has become normal. 8th 11.5 → November 8th 5.0 (reference value), ICA19-9 is June 8th 96 → November 8th 38. White blood cell counts are gradually increasing. The number of functions is maintained and the side effects are really light. "

  “A 39-year-old daughter was said to have colon cancer at a regular checkup in September and was immediately hospitalized. I got Ellen at the director's meeting. I was told that I was mistaken, and I was discharged immediately. I believe thanks to Ellen. "

(Rheumatic)
“Rheumatism. Since December last year, my hands, arms, and shoulders suddenly swelled, I couldn't sleep at night, moved, and had to endure. After that, I received an injection and a prescription, but the medicine I took did not fit my body. At present, I have two injections once a month.I don't know any ambiguity or smell because of side effects.I don't want remission, but I want to be lighter.I can only move by car in the countryside.If I can drive at least Happiness. ”→“ The pain disappeared on the 14th day. I was impressed when I woke up and there was no pain. From that day, there was no pain, and the teacher of Keio Hospital was “remission” at the November medical examination. I was saying. Life has become easier than I thought before drinking Ellen. "

  “Currently rheumatoid arthritis at the age of 52 and 28 years, and became severe. Liver function deteriorated as new drugs were administered, and the drug changed each time. The joints and hands of the legs bent, and deteriorated with age. I wanted to tell my experience because I was a nurse. ”→“ Light stiffness. Swelling and pain are calm. But there is pain when the weather is bad. Liver function values are normal. Inflammatory response is also normal. "→" There is no pain and the stiffness of my hands has disappeared. I've been struggling with pain for 23 years, but I'm really happy to have a painless life. "

(Autoimmune disease)
"Currently hospitalized in autoimmune disorders. Treatment is not standing prospect of steroid administration. Discharge." → "The value of ferritin did not fall at all, dropped to half from 8/22 and 9/1 at 782 → 386 Her physical condition is also very good. Liver function is normal. Comfortable stool, sleep well. Physical condition is also very good. "→" Comfortable. Good sleep, comfortable stool. Whatever you eat, delicious. Fun every day, no stress. Liver function values, ferritin, improvement. Discharge from the end of September. ”→“ Comfort. Liver function is dramatically better, now normal. ”

  “November 2013 surgery for lung cancer. Also suffered from psoriasis vulgaris for 30 years. Itchy face, waist, buttocks and legs are so severe that a large man wants to cry.” → “Liver numbers improved. Sheets. And the pajamas were bloody and laundered every day, but there was no 10 / 6.The belly circumference was clean and the head and neck were still there, but I wasn't tired, the feelings were calm, and I was fine.

(Other)
“When Joso flooded, the sewage was caught, and a sudden high fever continued for a week because of the bacteria at that time. After that, high fever began to appear frequently.” → “High fever once a week, but no longer I did not get out once in 3 months, the repetition of diarrhea and constipation was improved, the white blood cell level was normal, the skin was fine, the body weight decreased by 6 kg, the skin became white, and the hypothermia improved. "

  “Hepatitis C and colorectal cancer. I had surgery for colorectal cancer, but I wanted to improve immunity with banding.” → “I lost my dullness. My bowel movement was improved. I feel like it's the first time in a year. Thanks to Ellen. I think the test value has also improved. "→" I'm less tired, the color of the stool is normal. The pain is gone. I'm surprised that it ’s so light and so different. Hepatitis C is completely cured. ”

  “I have been suffering from irritable bowel syndrome and my gastrointestinal sensation has been painful since I was 15 years old, but I didn't bother. My neuralgia was about half as painful as before, making it easier to spend. Improved hypothermia.”

  “Parkinson's symptoms are mild. I can walk quickly. No cancer metastasis to the bladder. All blood tests are normal. I was surprised by the doctor that Parkinson could not think that his behavior would improve.”

  “The mouth became moist. My mouth was waking up many times in the night, but I started drinking and woke up drooling on the 4th day. I was impressed! Delicious."

  “The number of toilets in the night has decreased. Dementia has improved by more than 70%, and the people around me have been surprised.”

  “My lower body was numb and I couldn't put on my underwear, but now I can only walk when I sleep facing up, and I can walk. I've never been out for 10 years and 10 months, but on October 21st, I went out of Funabashi to Sahara 60 years ago and came back safely. "

  As described above, ingestion of the food compositions of Formulation Example 3 and Formulation Example 4 particularly improved symptoms such as cancer, rheumatism, autoimmune disease, and hepatitis C.

(Experiment 4)
The effects of Narine and Benix noctum extract on macrophage IL-12 production were measured in vitro.

(material)
-Cell name: J774.1 (JCRB0018) (macrophages)
Source: JCRB Cell Bank (HS Foundation)
Lot. No. : 01162014
Culture solution: RPMI 1640 containing 10% FBS and 1% P / S
Remarks: Adhered to mouse macrophage-like cell line, plate

・ Test substance Benix nokitake extract: Narine bacteria manufactured by Golden Biotechnology Corporation: VITAWAY Hesperidin, Armenian country: Broccoli powder manufactured by Hayashibara Co., Ltd .: Domestic broccoli micropowder manufactured by Kodama Foods Co., Ltd.

・ Main Reagents PRMI1640: Nacalai Tesque
10,000 U / ml Penicillin-10,000 μg / ml Streptmycin (P / S): Nacalai Tesque FBS: Gibco PBS: Nacalai Tesque
0.25% trypsin-EDTA: CpG-DNA from Nacalai Tesque, HC4033: IL-12 / IL-23 (p40) from Cosmobio, Nonallele-Specific, Mouse, ELISA kit, Quantikine:
Made by R & D

-Culture medium RPMI1640 was added to 50 ml of inactivated FBS and 5 ml of P / S.
-Preparation of CpG-DNA 0.2 ml of sterilized DW was added to 200 nmol of CpG-DNA lyophilized product, and completely dissolved by pipetting to prepare a 1 nmol / L (1 mM) solution.
-Preparation of test substance 10 ml of PBS was added to 100 mg of each test substance to prepare a 10 mg / ml solution.
Furthermore, using this solution and PBS and a culture medium, dilutions with final concentrations of 0.1 μg / ml, 1 μg / ml, 10 μg / ml, 100 μg / ml, and 1,000 μg / ml were prepared, respectively. did.

(Cell culture)
The cells are cultured in a carbon dioxide incubator (37 ° C., 5% CO ₂ ). For cell passage, after washing the cells with PBS (−), the cells are detached by trypsin-EDTA treatment. The detached cells are collected by adding a cell culture medium, and the cell density of the cell suspension is calculated using a fully automatic cell counter or a hemocytometer. The cell suspension is seeded in a culture flask and cultured in a carbon dioxide incubator (37 ° C., 5% CO ₂ ).

(IL-12 induction by CpG-DNA)
Inoculate J774.1 cells suspended in culture medium at 1 × 10 ⁵ / ml in a 48-well plate at 0.25 ml / well (0.25 × 10 ⁵ / well) and adhere completely to the plate. And overnight in a carbon dioxide incubator (37 ° C., 5% CO ₂ ). The culture solution was removed, and 0.25 ml of CpG-DNA (0, 0.01, 0.1, 1, 10 μM) serially diluted with the culture medium was added and cultured for 24 to 48 hours (2 per dilution rate). Well use, Appendix 1). After incubation, the sample was collected in an Eppendorf tube, centrifuged, and well samples under the same conditions were pooled and stored at −80 ° C. until evaluation of IL-12.

  Here, CpG-DNA is a continuous base sequence of unmethylated cytosine and guanine. CpG-DNA is a unique base sequence for bacteria and is recognized as a foreign substance in vertebrates such as humans. Therefore, if CpG-DNA is used, it can be investigated whether a macrophage is functioning normally.

  IL-12 is a cytokine that is produced by immune cells and leads to immune activation and antiallergic action. IL-12 activates NK cells and activates Th1 cells that lead to an antiallergic action, thereby contributing to alleviation of allergic symptoms. Therefore, by measuring the amount of IL-12 produced by macrophages, the immunostimulation and antiallergic action of each test substance can be confirmed.

(IL-12 induction by test substance alone)
Inoculate J774.1 cells suspended in culture medium at 1 × 10 ⁵ / mL in 48-well plate at 0.25 ml / well (0.25 × 10 ⁵ / well), and adhere completely to the plate And overnight in a carbon dioxide incubator (37 ° C., 5% CO ₂ ). Remove the culture solution, add 0.25 mL each of the four types of test substances (0, 1, 10, 100, 1,000 μg / ml) serially diluted and incubate for 24 to 48 hours (per dilution rate) 2well use, Appendix 1). After incubation, the sample was collected in an Eppendorf tube, centrifuged, and well samples under the same conditions were pooled and stored at −80 ° C. until evaluation of IL-12.

(IL-12 induction by a combination of Benix nokitake extract and Narine bacteria)
Inoculate J774.1 cells suspended in culture medium at 1 × 10 ⁵ / ml in a 48-well plate at 0.25 ml / well (0.25 × 10 ⁵ / well) and adhere completely to the plate. And overnight in a carbon dioxide incubator (37 ° C., 5% CO ₂ ). Based on the result evaluated in 11.6.2.2, a sample in which the amount of venix mushroom extract is changed with respect to a certain amount of narine bacteria having an amount of 1/2 to 1/10 that induces IL-12 at the maximum. (Appendix 2-1). The culture broth was removed, and 0.25 mL of Benix nokitake extract + Narine bacteria sample was added and cultured for 24 to 48 hours (2 wells used per dilution). After incubation, the sample was collected in an Eppendorf tube, centrifuged, and well samples under the same conditions were pooled and stored at −80 ° C. until evaluation of IL-12.

(Measurement of IL-12)
Mouse IL-12 activity was measured using 50 μL of a thawed sample (diluted as necessary) according to the protocol attached to IL-12 / IL-23 (p40), Nonallele-Specific, Mouse, ELISA Kit, Quantikine. .

(Result of Experiment 4)
FIG. 13 is a graph showing the IL-12 productivity of macrophages by a test substance alone. In FIG. 13A, the vertical axis represents the detected amount of IL-12, the horizontal axis represents the added amount of CpG-DNA, and in FIGS. 13B to 13E, the vertical axis represents the detected amount of IL-12. The horizontal axis represents the amount of test substance added. As shown in FIG. 13, each of Narine fungus, Benix nokitake extract, Hesperidin, and broccoli powder showed IL-12 induction performance against macrophages to some extent.

  Here, FIG. 13 (A) shows the induction of macrophages by CpG-DNA. As shown in FIG. 13 (A), the macrophages used in the experiment normally produced IL-12.

  FIG. 13 (B) is a diagram showing the induction of IL-12 of macrophages by Narine bacteria alone. As shown in FIG. 13 (B), Narine bacteria induced IL-12 most at a concentration of 1,000 μg / ml.

  FIG. 13 (C) is a diagram showing induction of macrophage IL-12 by Benix nokitake extract alone, and FIG. 13 (C1) is a diagram in which the vertical axis of FIG. 13 (C) is changed. As shown in FIG. 13 (C) and FIG. 13 (C1), Benix nokitake extract most induced IL-12 at a concentration of 100 μg / ml.

  FIG. 13 (D) is a diagram showing induction of macrophage IL-12 by hesperidin alone, and FIG. 13 (D1) is a diagram in which the vertical axis of FIG. 13 (D) is changed. As shown in FIG. 13 (D) and FIG. 13 (D1), hesperidin induced IL-12 most at a concentration of 10 μg / ml.

  FIG. 13 (E) is a diagram showing the induction of macrophage IL-12 by broccoli powder alone, and FIG. 13 (E1) is a diagram in which the vertical axis of FIG. 13 (E) is changed. As shown in FIG. 13 (E) and FIG. 13 (E1), broccoli powder most induced IL-12 at a concentration of 1,000 μg / ml.

  Next, the induction of IL-12 by a combination of Benix nokitake extract and Narine bacteria was measured. FIG. 14 shows the induction of macrophages by CpG-DNA. In FIG. 14, the vertical axis represents the detected amount of IL-12, and the horizontal axis represents the amount of CpG-DNA added. As shown in FIG. 14, the macrophages used in the experiment normally produced IL-12.

  FIG. 15 is a figure which shows the result of IL-12 induction | guidance | derivation by the combination of a Benicus mushroom extract and Narine bacteria. In FIG. 15, 1 on the horizontal axis is unstimulated (control), 2 is added with 1,000 μg / ml of Benicus mushroom extract, 3 is added with 500 μg / ml of Narine bacteria, 4 is 500 μg / ml of Narine bacteria, and Benixoki. Addition of bamboo extract 1 μg / ml, 5 indicates addition of 500 μg / ml of Narine bacteria and 10 μg / ml of Benix nokitake extract, and 6 indicates addition of 500 μg / ml of Narine bacteria and 100 μg / ml of Benix nokitake extract, respectively.

  As shown in FIG. 15, IL-12 induction of macrophages was more activated by the combination of Benicus nokitake extract and Narine bacteria. In particular, the induction of IL-12 was most activated when 500 μg / ml of Narine bacteria shown in 5 and 10 μg / ml of Benix nokitake extract were added.

  As described above, with regard to Formulation Example 3 and Formulation Example 4, remarkable improvement in bodily sensation was observed particularly for subjects with a history of cancer and immune system diseases. Therefore, the food composition of the present embodiment has an effect of remarkably contributing to normalization of immune function.

  Specifically, in addition to cancer, effects of improving various symptoms were observed for rheumatism, autoimmune disease, and hepatitis C. Therefore, the food composition of the present embodiment can be used for alleviating various symptoms of these diseases.

Claims (7)

Benix nokitake and
Narine bacteria,
A food composition comprising:   The food composition according to claim 1, further comprising broccoli.   The food composition according to claim 1 or 2, further comprising hesperidin.   The food composition according to any one of claims 1 to 3, wherein the use is normalization of immune function.   The food composition according to any one of claims 1 to 3, wherein the use is for alleviation of various symptoms of cancer.   The food composition according to any one of claims 1 to 3, wherein the use is for alleviation of various symptoms of autoimmune diseases.   The food composition according to any one of claims 1 to 3, wherein the use is for alleviation of various symptoms of rheumatism.