JP6501268B2 - Method of producing Bacillus spray liquid - Google Patents
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- JP6501268B2 JP6501268B2 JP2016145009A JP2016145009A JP6501268B2 JP 6501268 B2 JP6501268 B2 JP 6501268B2 JP 2016145009 A JP2016145009 A JP 2016145009A JP 2016145009 A JP2016145009 A JP 2016145009A JP 6501268 B2 JP6501268 B2 JP 6501268B2
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- 241000193830 Bacillus <bacterium> Species 0.000 title claims description 49
- 239000007921 spray Substances 0.000 title claims description 27
- 239000007788 liquid Substances 0.000 title claims description 26
- 238000000034 method Methods 0.000 title description 8
- 238000005273 aeration Methods 0.000 claims description 25
- 239000011259 mixed solution Substances 0.000 claims description 16
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 12
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 12
- 239000002244 precipitate Substances 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 239000000356 contaminant Substances 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 230000000249 desinfective effect Effects 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 239000010802 sludge Substances 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 238000004065 wastewater treatment Methods 0.000 description 9
- 230000001877 deodorizing effect Effects 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000004332 deodorization Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000010800 human waste Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000223935 Cryptosporidium Species 0.000 description 1
- 241000224466 Giardia Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000005654 stationary process Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Apparatus For Disinfection Or Sterilisation (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
- Treatment Of Sludge (AREA)
- Cultivation Of Plants (AREA)
- Housing For Livestock And Birds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
本発明は、脱臭などに用いるため、消毒した小規模排水処理施設の曝気槽混合液からバチルス菌を取り出してバチルス噴霧液を製造する方法に関するものである。 The present invention relates to a method for producing a bacillus spray liquid by removing Bacillus bacteria from the aerated tank mixed solution of a disinfected small scale wastewater treatment facility for use in deodorization and the like.
排水処理施設では水処理の結果、汚泥が大量に発生する。汚泥は水分を多量に含んだ液状で取り出され、多量の有機分を含んでいるため不安定ですぐ腐敗による悪臭が発生するなど、取扱いが困難であり、この汚泥を処理する場合、減量化し安定化することが必要である。一般的には汚泥を濃縮、消化、脱水、焼却というステップで処理しているが、廃棄される汚泥を肥料や建築資材などに再利用する技術も近年開発されている。 Wastewater treatment facilities generate large amounts of sludge as a result of water treatment. Sludge is taken out in a liquid form containing a large amount of water, and contains a large amount of organic components, making it unstable and causing an offensive odor immediately after rot, etc., making it difficult to handle. Needs to be Generally, sludge is treated in the steps of concentration, digestion, dewatering and incineration, but in recent years, technology for reusing discarded sludge for fertilizers and building materials has also been developed.
ところで、養豚農家などでは畜舎から発生する臭気の発生防止対策が求められており、大規模で高性能の糞尿処理設備が導入されている例が多い。しかしながら、高額な費用をかけて脱臭装置を設けても、臭気を完全に除去することはできず、周辺住民から苦情が来ることもあり、脱臭装置を設けていても畜舎内に脱臭剤を散布するなどの対策が講じられている。 By the way, a pig farmer or the like is required to take measures to prevent the generation of an odor generated from a barn, and there are many cases where large-scale, high-performance manure processing equipment has been introduced. However, even if deodorizing equipment is installed at high cost, the odor can not be completely removed, and there may be complaints from nearby residents, so even if deodorizing equipment is installed, the deodorizing agent is sprayed in the barn Measures have been taken.
バチルス菌を用いた脱臭方法は従来から良く知られており、例えば特許文献１にはバチルス菌を含む液体消臭剤をトイレの汚水タンクに適当な濃度で添加したり、生ごみなどに直接振りかけたりする方法が開示されている。 Deodorization methods using bacillus bacteria are well known in the prior art, for example, in Patent Document 1, a liquid deodorant containing bacillus bacteria is added to a waste water tank of a toilet at an appropriate concentration, or sprinkled directly on garbage etc. Methods are disclosed.
しかしながら、畜舎内に脱臭用バチルス菌を噴霧するとしても、現在販売されているバチルス菌の噴霧液は高額であり、多用するのが難しいという問題点がある。発明者は小規模排水処理施設、特に農業集落排水施設の余剰汚泥を分析し、バチルス菌が優占化していることを発見した。汚泥滞留時間の長いし尿処理施設では優占化の例が観察されるが、滞留時間の短い小規模な排水処理施設でバチルス優占化が進んでいるのは珍しい例である。 However, even if deodorizing Bacillus bacteria are sprayed in a barn, the spray solution of Bacillus bacteria currently sold is expensive, and there is a problem that it is difficult to frequently use. The inventor analyzed the excess sludge of a small scale wastewater treatment facility, particularly an agricultural settlement drainage facility, and found that Bacillus bacteria were dominant. An example of dominance is observed in a human waste treatment facility with a long sludge retention time, but it is a rare case that the dominance of bacillus is advanced in a small scale drainage treatment facility with a short residence time.
そこで、本発明においては本来廃棄する余剰汚泥からバチルス噴霧液を製造することにより、安価なバチルス噴霧液を製造することを目的とする。 Therefore, in the present invention, an object of the present invention is to produce a cheap Bacillus spray solution by producing a Bacillus spray solution from excess sludge originally discarded.
本発明は、農業集落排水施設の曝気槽混合液を取得する工程と、前記曝気槽混合液に対して２５０ｍｇ／Ｌ〜２５００ｍｇ/Ｌの濃度となるように次亜塩素酸ナトリウムを添加して消毒を行う消毒工程と、消毒後の曝気槽混合液を静置して沈殿物を取得する工程と、前記沈殿物中の夾雑物を除去する工程と、を有し、前記夾雑物が除去された沈殿物がバチルス噴霧液となることを特徴とするバチルス噴霧液の製造方法により、上記課題を解決する。
The present invention comprises the steps of obtaining an aeration tank mixed solution of agricultural settlement drainage facility , and adding and disinfecting sodium hypochlorite to a concentration of 250 mg / L to 2500 mg / L with respect to the aeration tank mixed solution a disinfecting step of performing the steps of acquiring the aeration tank mixture precipitates on standing after disinfection, have a, a step of removing the contaminants in the precipitate, the contaminants have been removed The above problems are solved by the method for producing a bacillus spray liquid, wherein the precipitate is a bacillus spray liquid .
本発明に係るバチルス噴霧液の製造方法によれば、本来廃棄する余剰汚泥を利用して製造するので原材料費が不要であり、バイオマス資源の循環利活用の面からもモデルケースになる。そして、本発明によれば市販の液体バチルスに対して大幅に安価で長期保存が可能なバチルス噴霧液を製造することができる。また、本発明により得られるバチルス噴霧液を農業に利用した場合、野菜の消毒や連作障害抑止にも効果がある。 According to the method for producing a bacillus spray liquid according to the present invention, since it is produced utilizing surplus sludge originally discarded, no raw material cost is required, and it is a model case also from the aspect of recycling and utilization of biomass resources. And according to the present invention, it is possible to produce a bacillus spray liquid which is significantly cheaper than a commercially available liquid bacillus and which can be stored for a long time. Moreover, when the bacillus spray liquid obtained by this invention is utilized for agriculture, it is effective also in disinfection of vegetables, and continuous crop disorder suppression.
本発明に係るバチルス噴霧液の製造方法の実施の形態について図面に基づいて説明する。ここで、図１は本発明に係るバチルス噴霧液の製造方法の模式図である。バチルス菌とは枯草菌（Ｂａｃｉｌｌｕｓ ｓｕｂｔｉｌｉｓ）であり、稲藁などに付着する菌である。また、バチルス菌はカビや細菌など、植物の病気を引き起こす２８種の病原菌を殺菌する能力を持つことが知られている。バチルス噴霧液は、消臭効果の他、土壌改善、連作障害などにも効果がある。 An embodiment of a method for producing a Bacillus spray liquid according to the present invention will be described based on the drawings. Here, FIG. 1 is a schematic view of a method for producing a Bacillus spray liquid according to the present invention. Bacillus bacteria are Bacillus subtilis and are bacteria that adhere to rice straw and the like. In addition, Bacillus bacteria are known to have the ability to kill 28 pathogens that cause plant diseases such as mold and bacteria. The Bacillus spray solution is effective not only for deodorizing effect but also for soil improvement, continuous crop failure and the like.
本発明に係るバチルス噴霧液を製造方法するため、まず、小規模排水処理施設の曝気槽から混合液を取得する。小規模排水処理施設とは、処理人数が公共下水道に比べて小規模な場合に設けられる排水処理施設全般のことであり、農業集落排水施設、漁業集落排水施設、林業集落排水施設、簡易排水施設、小規模集合排水処理施設、コミュニティプラント、合併浄化槽をはじめとする排水処理施設を指す。この施設内における曝気槽での滞留時間は公共下水道のし尿処理施設に比して比較的短い。そして、この曝気槽で発生する余剰汚泥、特に農業集落排水施設で発生する余剰汚泥はバチルス菌が優占化していることを発明者は発見した。そのため、農業集落排水施設の曝気槽混合液を使用することが望ましい。農業集落排水施設には工業排水が流入することはないので、安全性の高いバチルス噴霧液の製造が期待できる。 In order to produce the Bacillus spray liquid according to the present invention, first, the mixed liquid is obtained from the aeration tank of a small scale wastewater treatment facility. Small-scale wastewater treatment facility refers to the entire wastewater treatment facility provided when the number of people treated is smaller than that of a public sewer, and includes agricultural settlement drainage facilities, fishery settlement drainage facilities, forestry settlement drainage facilities and simplified drainage facilities. , Refers to wastewater treatment facilities including small-scale collective wastewater treatment facilities, community plants, and combined septic tanks. The residence time in the aeration tank in this facility is relatively short compared to the human waste treatment facility of the public sewer. Then, the inventor discovered that the excess sludge generated in the aeration tank, particularly the excess sludge generated in the agricultural settlement drainage facility, is dominated by Bacillus bacteria. Therefore, it is desirable to use the aeration tank mixed solution of agricultural settlement drainage facilities. Industrial drainage does not flow into agricultural settlement drainage facilities, so it can be expected to produce Bacillus spray liquid with high safety.
そこで、これらの施設から曝気槽混合液を取得する工程により、バチルス菌が優占化した余剰汚泥を得る。曝気槽混合液取得時のバチルス菌数は１×１０６個／g以上、好ましくは１×１０７〜１×１０８個／g、であることが望ましい。この時点では市販されている液体バチルスのバチルス菌数の１／７程度に過ぎないが、この後、曝気槽混合液を消毒し濃縮するので、最終的には液体バチルスと同等の濃度で使用することが可能になる。 Therefore, excess sludge in which Bacillus bacteria are dominant is obtained by the process of obtaining the aeration tank mixed solution from these facilities. The number of Bacillus bacteria at the time of obtaining the aeration tank mixed solution is desirably 1 × 10 6 cells / g or more, preferably 1 × 10 7 to 1 × 10 8 cells / g. At this point, it is only about 1/7 the number of Bacillus bacteria in the commercially available liquid bacillus, but after that, the aeration tank mixed solution is disinfected and concentrated, so it is finally used at the same concentration as liquid bacillus It becomes possible.
当該曝気槽混合液には大腸菌やウイルスなど、人体に有害な細菌やウイルスをはじめ、ジアルジアやクリプトスポルディウムなどの耐塩素性原虫類が含まれている。そのため、次に前記曝気槽混合液に次亜塩素酸ナトリウムを高濃度に添加し攪拌して消毒を行う。これが消毒工程である。攪拌は攪拌機等を用い、所定の時間行う。その濃度についていえば、次亜塩素酸ナトリウムの濃度が前記曝気槽混合液に対して２５０ｍｇ／Ｌ〜２５００ｍｇ/Ｌ、望ましくは８００ｍｇ／Ｌ〜１０００ｍｇ/Ｌとなるように添加する。このように健康リスクを引き起こす微生物を消毒し死滅させることができる程度に次亜塩素酸ナトリウムを添加し、攪拌して消毒を行う。 The aeration tank mixed solution contains bacteria and viruses harmful to human body such as Escherichia coli and viruses, and chlorine resistant protozoa such as Giardia and Cryptosporidium. Therefore, next, sodium hypochlorite is added to the aeration tank mixed solution to a high concentration, and the mixture is stirred and disinfected. This is the disinfection process. Stirring is performed for a predetermined time using a stirrer or the like. As for the concentration, sodium hypochlorite is added to the aeration tank mixed solution at 250 mg / L to 2500 mg / L, preferably 800 mg / L to 1000 mg / L. As such, sodium hypochlorite is added to the extent that microbes causing health risks can be disinfected and killed, and then disinfected by stirring.
一方、バチルス菌は芽胞形成菌なので、消毒時に芽胞を形成して生き残ることができるため、人体に有害な微生物のみ死滅させ、バチルス菌を残存させておくことが可能になる。 On the other hand, since bacillus bacteria are spore-forming bacteria, they can form spores and survive during disinfection, so that only microorganisms harmful to the human body can be killed and bacillus bacteria can be left.
表１は曝気槽混合液を採取し、次亜塩素酸ナトリウムの濃度と大腸菌数及びバチルス菌数の関係を示したものである。試料１〜試料４の曝気槽混合液を取得する小規模排水処理施設は同一であるが、採取日は異なる。試料１〜試料４に対し、次亜塩素酸ナトリウムの添加率を変化させて、それぞれの大腸菌数とバチルス菌数を測定した。なお、曝気槽混合液５００ｍｌに次亜塩素酸ナトリウムを加え、ジャーテスタで攪拌した後、採泥したものを測定している。大腸菌数とバチルス菌数は乾燥重量当たりの個数を表す。この表から明らかなように、次亜塩素酸ナトリウムの添加率が６００ｍｇ／Ｌまでは、バチルス菌数がほとんど影響を受けないことが分かる。また、次亜塩素酸ナトリウムの添加率が２００ｍｇ／Ｌ以上でないと大腸菌が死滅しないこともわかる。さらに、次亜塩素酸ナトリウムの添加率が１２００ｍｇ／Ｌでも１０７個／ｇの値を示しており、数多く残存している。この注入率で消毒した混合液であれば、病原細菌・ウイルスを含めて消毒されており、噴霧時の健康リスクは少ない。 Table 1 shows the relationship between the concentration of sodium hypochlorite and the number of E. coli and the number of Bacillus bacteria by collecting the aeration tank mixed solution. Although the small-scale waste water treatment facility which acquires the aeration tank liquid mixture of sample 1-sample 4 is the same, collection dates differ. The addition rate of sodium hypochlorite was changed with respect to samples 1 to 4, and the number of E. coli and the number of Bacillus bacteria were measured. In addition, after adding sodium hypochlorite to 500 ml of aeration tank liquid mixture and stirring it with a jar tester, what was collected is measured. The number of E. coli and the number of Bacillus bacteria represent the number per dry weight. As apparent from this table, it can be seen that the number of Bacillus bacteria is hardly affected until the addition rate of sodium hypochlorite is 600 mg / L. In addition, it is also found that E. coli does not die unless the addition rate of sodium hypochlorite is 200 mg / L or more. Furthermore, even if the addition rate of sodium hypochlorite is 1200 mg / L, the value of 10 7 pieces / g is shown, and many remain. A mixed solution disinfected at this injection rate is disinfected including pathogenic bacteria and viruses, and there is little health risk at the time of spraying.
次に、消毒後の曝気槽混合液を静置すると、沈殿物と上澄み液に分離するので、その沈殿物を取得する。この沈殿物はバチルス菌が優占化した泥であり、これがバチルス菌の噴霧液の原料となるものである。消毒済であるから健康リスクを生じさせることもない。また、バチルス菌が優占化されて臭いもない。１日〜２日静止させると分離して沈殿物が取得できるようになる。このように消毒済みの曝気槽混合液を沈殿させることで濃縮し、高濃度のバチルス噴霧液の原液を得ることを可能とする。 Next, when the aeration tank mixed liquid after sterilization is allowed to stand, it separates into a precipitate and a supernatant, so the precipitate is obtained. The precipitate is mud which is dominated by Bacillus bacteria, and this is the raw material of the Bacillus subtilis spray solution. Because it has been disinfected, it does not pose a health risk. In addition, the Bacillus bacteria are dominant and there is no smell. If it is stopped for 1 day to 2 days, it will separate and it will be possible to obtain a deposit. As described above, the disinfected aeration tank mixture is concentrated by settling to make it possible to obtain a stock solution of a high concentration of Bacillus spray solution.
次に、前記沈殿物中の夾雑物を除去する。もともと曝気槽混合液は生活排水であるから、トイレットペーパーなどの繊維が消毒後も残存している。そのまま噴霧液に用いると、高圧噴霧器が閉塞するため、篩などの濾過手段を用いて夾雑物を除去する。１００〜３００μｍの目幅の篩を使用することができる。これにより夾雑物は除去され、透過した泥を含む沈殿物をバチルス噴霧液として使用することができる。 Next, the impurities in the precipitate are removed. Originally, since the aeration tank mixed liquid is a domestic drainage, fibers such as toilet paper remain even after disinfection. If used as a spray liquid as it is, the high pressure sprayer is clogged up, and thus the contaminants are removed using a filtering means such as a sieve. A sieve with an eye width of 100 to 300 μm can be used. Contaminants are thereby removed, and the sediment containing the permeated mud can be used as a Bacillus spray.
こうして得られた噴霧液のバチルス菌数が１×１０４ 〜１×１０６個／ｍｌ、であることが望ましい。その後、噴霧液を希釈してバチルス噴霧液として使用する。例えば２０倍希釈などで噴霧することができる。希釈後の噴霧液中に、バチルス菌数が１×１０３ 〜１×１０５個／ｍｌ存在していれば、消臭効果が期待できるので、その割合まで希釈することができる。畜舎などに散布すれば消臭効果を期待でき、農業に用いれば土壌改良の他、葉面に散布すれば消毒を促進することができる。 The number of Bacillus bacteria in the spray solution thus obtained is preferably 1 × 10 4 to 1 × 10 6 cells / ml. Thereafter, the spray solution is diluted and used as a Bacillus spray solution. For example, it can spray by 20 times dilution etc. If the number of Bacillus bacteria is 1 × 10 3 to 1 × 10 5 pieces / ml in the spray solution after dilution, the deodorizing effect can be expected, so that the ratio can be diluted. It can be expected to have a deodorizing effect if it is applied to a barn or the like, and if it is used for agriculture it is possible to promote disinfection if it is applied to the foliage besides soil improvement.
本発明に係る実施例を説明する。ＭＬＳＳ濃度２９００ｍｇ／Ｌ、バチルス菌数が１×１０６個／ｍｌ（乾燥重量当たりで３．４×１０８個／ｇ）の農業集落排水処理施設の曝気槽混合液を取得した。次に前記曝気槽混合液１５リットルに次亜塩素酸ナトリウムを１２００ｍｇ／Ｌの濃度で攪拌し混合する。そしてこの混合液を静止させて下に沈殿した混合液２リットルを得る。これを３００μｍ目幅の篩で濾過し濾過液を回収保存する。この混合液は市販の液体バチルスと同等のバチルス菌濃度となるので、消毒効果が期待できる。 An embodiment according to the present invention will be described. An aeration tank mixed solution of an agricultural settlement drainage treatment facility having an MLSS concentration of 2900 mg / L and a Bacillus count of 1 × 10 6 cells / ml (3.4 × 10 8 cells / g on a dry weight basis) was obtained. Next, sodium hypochlorite is stirred and mixed at a concentration of 1200 mg / L in 15 liters of the aeration tank mixed solution. The mixture is then allowed to stand and 2 liters of mixture which has precipitated down are obtained. This is filtered with a sieve of 300 μm mesh width, and the filtrate is recovered and stored. Since this mixed solution has a Bacillus cell concentration equivalent to that of a commercially available liquid Bacillus, a disinfecting effect can be expected.
Ｓ１０１ 曝気槽混合液取得工程
Ｓ１０２ 消毒工程
Ｓ１０３ 静置工程
Ｓ１０４ 夾雑物除去工程
S101 Aeration tank mixed liquid acquisition process
S102 disinfection process
S103 stationary process
S104 Contaminant removal process
Claims (1)
前記曝気槽混合液に対して２５０ｍｇ／Ｌ〜２５００ｍｇ/Ｌの濃度となるように次亜塩素酸ナトリウムを添加して消毒を行う消毒工程と、
消毒後の曝気槽混合液を静置して沈殿物を取得する工程と、
前記沈殿物中の夾雑物を除去する工程と、
を有し、前記夾雑物が除去された沈殿物がバチルス噴霧液となることを特徴とするバチルス噴霧液の製造方法。 Obtaining the aeration tank mixed liquid of the agricultural settlement drainage facility ,
A disinfecting step of disinfecting by adding sodium hypochlorite to a concentration of 250 mg / L to 2500 mg / L with respect to the aeration tank mixed solution ;
Allowing the aeration tank mixture after sterilization to stand to obtain a deposit;
Removing contaminants in the precipitate;
Have a method for producing a Bacillus spray liquor precipitate the contaminants have been removed, characterized by comprising a Bacillus spray.
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