JP6550673B2 - Photoluminescence lifetime measuring apparatus and measuring method - Google Patents

Description

  The present invention relates to techniques for measuring the lifetime of photoluminescence, eg phosphorescence.

  Conventionally, it has been attempted to measure the oxygen partial pressure in a living body as a method for observing the condition of the living body. For example, when there is a cancer tissue in a living body, the oxygen partial pressure in that portion becomes lower than that of surrounding normal cells. Therefore, by measuring the partial pressure of oxygen, for example, it becomes possible to observe the presence or absence and spread of cancer tissue.

  As a method for measuring the partial pressure of oxygen in the living body, there is known one which introduces a certain probe into the living body and measures the intensity of phosphorescence produced by light irradiation of the probe. In this case, the relationship between the phosphorescence intensity and the oxygen partial pressure is experimentally obtained in advance, for example, a calibration curve is created. Then, the oxygen partial pressure is estimated from the obtained phosphorescence intensity.

  However, the phosphorescence intensity also depends on the probe concentration. Especially in the living body, it is difficult to obtain an accurate probe concentration, so the reliability of the obtained partial pressure of oxygen was questionable.

  Therefore, Non-Patent Document 1 below proposes a technique using an iridium complex as a probe. According to this, based on the relationship between the lifetime of phosphorescence generated in the iridium complex and the oxygen partial pressure, it is possible to determine the oxygen partial pressure without depending on the probe concentration.

  Moreover, the measurement technique of the oxygen partial pressure which used the phosphorescence lifetime is also described in the following patent document 1.

JP 2007-232716 A

Toshitada Yoshihara, Atsushi Kobayashi, Shinpei Oda, Masahiro Hosaka, Toshiyuki Takeuchi, Seiji Tobita, "Iridium complex probes for monitoring of cellular oxygen levels and imaging of hypoxic tissues", Proc. Of SPIE 8233, 82330A-1 (2 February 2012)

  By the way, in the above-described conventional technique, it is necessary to use a special equipment such as a photon counter in order to measure the phosphorescence lifetime, in addition to the normal microscope for ecological observation. For this reason, there exists a problem that it becomes expensive as the whole apparatus. Moreover, in the prior art, there is also a problem that it is difficult to accurately measure the oxygen partial pressure which fluctuates dynamically in the living body because the time required to measure the life is long.

  The present invention has been made based on the above findings. The main object of the present invention is to provide a technique capable of reducing the cost of the apparatus and capable of quickly measuring the lifetime of photoluminescence (phosphorescence or fluorescence).

  Means for solving the above-described problems can be described as follows.

(Item 1)
It has an image acquisition unit and an analysis unit,
The image acquisition unit includes a first scanning unit and a recording unit.
The first scanning unit is configured to acquire a first image of the object by scanning the object in at least one direction.
The recording unit is configured to record the acquired first image,
A photoluminescence life measuring apparatus, wherein the analysis unit is configured to calculate a life of photoluminescence in the object by calculating a shake function in the one direction for the first image.

(Item 2)
Furthermore, a second scanning unit is provided,
The second scanning unit is configured to acquire a second image of the object by scanning in the same direction as the scanning direction by the first scanning unit,
The scanning speed in the second scanning unit is lower than the scanning speed in the first scanning unit,
The recording unit is configured to record the acquired second image,
The photoluminescence lifetime measuring apparatus according to Item 1, wherein the analysis unit is configured to further use image information in the second image in order to calculate the lifetime of the photoluminescence in the object.

(Item 3)
Furthermore, it has a multiphoton excitation unit,
The photoluminescence lifetime measuring device according to Item 1 or 2, wherein the multiphoton excitation unit is configured to cause light emission by a multiphoton absorption process to a probe disposed in the object.

(Item 4)
The photoluminescence lifetime measuring apparatus according to any one of Items 1 to 3, wherein the first scanning unit is configured using a resonant scanner capable of scanning in at least the one direction.

(Item 5)
The photoluminescence lifetime measuring apparatus according to any one of Items 1 to 4, wherein the second scanning unit is configured using a galvano scanner capable of scanning in at least the one direction.

(Item 6)
The analysis unit is configured to calculate a shake function in one direction with respect to a divided image obtained by dividing the first image. The photoluminescence life measurement according to any one of items 1 to 5. apparatus.

(Item 7)
Furthermore, it has a lifetime image generation unit and a presentation unit,
The lifetime image generation unit is configured to generate a lifetime image representing the lifetime calculated by the analysis unit,
The photoluminescence lifetime measuring apparatus according to any one of Items 1 to 6, wherein the presentation unit is configured to present the lifetime image to a user.

(Item 8)
Furthermore, a second scanning unit is provided,
The second scanning unit is configured to acquire a second image of the object by scanning in the same direction as the scanning direction by the first scanning unit,
The second scanning unit includes an anti-blur unit for preventing the blur of the second image,
The recording unit is configured to record the acquired second image,
The photoluminescence lifetime measuring apparatus according to Item 1, wherein the analysis unit is configured to further use image information in the second image in order to calculate the lifetime of the photoluminescence in the object.

(Item 9)
The image sampling interval for acquiring the first image is longer than the image sampling interval at which blurring due to a point spread function (PSF) can be observed. Photoluminescence according to any one of items 1 to 8. Life measuring device.

(Item 10)
Using the photoluminescence lifetime measuring device according to any one of items 1 to 9,
The first scanning unit acquiring a first image of the object by scanning the object in at least one direction;
Recording the acquired first image by the recording unit;
Calculating the life of the photoluminescence in the object by calculating the shake function in the one direction for the first image by the analysis unit.

(Item 11)
Comprising the photoluminescence lifetime measuring device according to any one of items 1 to 9 and an oxygen partial pressure calculating unit;
The oxygen partial pressure calculation unit is configured to calculate an oxygen partial pressure in the object using the calculated lifetime of the photoluminescence.

  According to the photoluminescence lifetime measuring apparatus according to the present invention, since it is not necessary to use special equipment such as a photon counter, the cost of the apparatus can be reduced. In addition, according to the apparatus of the present invention, it is possible to quickly measure the lifetime of photoluminescence (phosphorescence or fluorescence) based on a blur function calculated from an image obtained by scanning.

It is a block diagram showing a schematic structure of a photoluminescence life measuring device concerning one embodiment of the present invention. It is a flowchart for demonstrating the measuring method using the measuring apparatus of FIG. It is explanatory drawing for demonstrating notionally the image obtained by a low-speed scan. It is explanatory drawing for demonstrating notionally the image obtained by high-speed scanning. It is explanatory drawing for demonstrating notionally operation which divides | segments a 1st or 2nd image and obtains a divided image. It is explanatory drawing for demonstrating the calculation procedure of a blurring function. It is a figure which shows the example of the 2nd image without blurring . It is a figure which shows the specific example of a 1st image with blurring . It is a figure which shows the example which divided | segmented the 2nd image of FIG. It is a figure which shows the example which divided | segmented the 1st image of FIG.

  Hereinafter, a photoluminescence life measuring apparatus according to an embodiment of the present invention will be described with reference to the attached drawings.

(Configuration of the embodiment)
A photoluminescence life measuring apparatus (hereinafter, sometimes simply referred to as “measuring apparatus”) according to the present embodiment includes an image acquiring unit 10 and an analyzing unit 20 as main components. Furthermore, this measuring apparatus additionally includes a multiphoton excitation unit 30, a lifetime image generation unit 40, and a presentation unit 50.

  The image acquisition unit 10 includes a first scanning unit 11, a second scanning unit 12, and a recording unit 13.

  The first scanning unit 11 is configured to acquire a first image of the object 1 by scanning the object 1 in at least one direction. The first scanning unit 11 of the present embodiment is configured using a resonant scanner capable of high-speed scanning in both left and right directions. The resonant scanner acquires a line-shaped image in the X direction (width direction) by vibrating a mirror using a resonance phenomenon. Further, an XY image can also be acquired by shifting the scanning start position in the Y direction (height direction). Therefore, the first image in the present embodiment is used to include a line-shaped image acquired by a scanner.

  The second scanning unit 12 is configured to acquire a second image of the object 1 by scanning in the same direction as the scanning direction by the first scanning unit 11. The scanning speed in the second scanning unit 12 is slower than the scanning speed in the first scanning unit 11. The second scanning unit 12 in this embodiment is configured using a galvano scanner capable of scanning in both the left and right directions. The galvano scanner acquires a line-shaped image in the X direction (width direction) using a so-called galvano mirror which is reciprocated by an actuator. Further, an XY image can also be acquired by shifting the scanning start position in the Y direction (height direction). Accordingly, the second image in the present embodiment is used to include a line-shaped image acquired by a scanner, as in the case of the first image. Here, the second scanning unit 12 can scan an object 1 at the same position and direction as the first scanning unit 11 to acquire an image. Specifically, the second scanning unit 12 can simultaneously scan an object at the same position as the scanning position in the first scanning unit 11 via the half mirror 14 (see FIG. 1).

  The recording unit 13 is configured to record the first image acquired by the first scanning unit 11 and the second image acquired by the second scanning unit 12. Each of the first image and the second image may be recorded in units of line images acquired by a scanner.

  The analysis unit 20 is configured to calculate the lifetime of the photoluminescence of the object 1 by calculating the shake function in one direction (scanning direction) of the first image. Here, the blur function in one direction means a blur function corresponding to a pixel value in one direction or a waveform thereof in this embodiment. An example of calculating the blur function will be described later.

  Specifically, the analysis unit 20 of the present embodiment includes an image division unit 21, a shake function calculation unit 22, a life calculation unit 23, and an oxygen partial pressure calculation unit 24.

  The image dividing unit 21 is a functional element that generates divided images for the first image and the second image by dividing the first image and the second image into a plurality of regions.

  The shake function calculation unit 22 is configured to calculate a shake function in the first image and the second image using the first image and the second image (more specifically, divided images generated therefrom) ( Later). That is, the analysis unit 20 of this example uses the image data in the second image in calculating the blur function for the first image. Further, the analysis unit 20 of this example is configured to calculate a blur function in at least one direction for a divided image obtained by dividing the first image (described later).

  The lifetime calculation unit 23 is configured to calculate the lifetime of phosphorescence using the obtained blur function. The oxygen partial pressure calculation unit 24 is configured to calculate the oxygen partial pressure using the obtained phosphorescence lifetime. The detailed operation of the life calculation unit 23 will also be described later.

  The multiphoton excitation unit 30 is configured to cause the probe disposed inside the object 1 to emit light by a multiphoton absorption process. Such a multiphoton excitation unit can be configured, for example, in the same manner as a pump laser used in a conventional two-photon microscope, and thus detailed description thereof will be omitted. When a two-photon microscope is used, a pinhole as used in a confocal microscope can be omitted, so that a blurred image as described later can be taken.

  The lifetime image generation unit 40 is configured to generate a lifetime image (described later) representing the lifetime calculated by the analysis unit 20.

  The presentation unit 50 is configured to present a lifetime image to the user. The presentation unit 50 is not particularly limited as long as it can present an image to the user, such as an LCD display or a printer. In addition, the presentation unit 50 may be configured to provide presentation data to an external system.

(Operation of Measuring Device of Embodiment)
Next, the operation of the measurement apparatus according to the present embodiment will be described with further reference to FIG.

(Step SA-1 in FIG. 2)
First, when performing the measurement method in the present embodiment, a light emitting material (phosphorescent material in the present embodiment) to be a probe is supplied to the target 1 in advance. As such a luminescent material, for example, an iridium complex (see Non-Patent Document 1) is known, but is not limited thereto. For example, when measuring the luminescence lifetime in a living body, the probe is administered into the living body by an appropriate means such as injection.

  Next, the multi-photon excitation unit 30 irradiates the object with light (that is, laser light) that causes light emission through a multi-photon absorption process in the probe. Such a method of generating and transmitting excitation light is well known from the prior art, and thus the detailed description is omitted. In the present embodiment, phosphorescence can be generated in the probe arranged on the object 1 by irradiation with excitation light.

Simultaneously with or immediately after the excitation light irradiation described above, the first scanning unit 11 of the image acquisition unit 10 acquires a first image while scanning the object 1. Here, if the scanning speed of the first scanning unit 11 is slow, adjacent pixel positions can not be scanned until the luminescence of phosphorescence is attenuated, and the phosphorescence at one point is simply point 3 ( It is observed as a schematic diagram of FIG. However, in the present embodiment, since the scanning speed of the first scanning unit 11 is sufficiently high, adjacent pixel positions can be scanned before the emission of phosphorescence is attenuated. Then, afterglow is observed even at pixel positions that are not originally illuminated. That is, the first image obtained by the first scanning unit 11 of the present embodiment, corresponding to the lifetime of phosphorescence, see schematic diagram of the scanning direction blanking Les 4 stretched (to the right in FIG. 4) (Fig. 4 ) Will be. The obtained first image of one line is recorded by the recording unit 13. Similarly, images can be recorded line by line. Also in the case of so-called bidirectional scanning in which scanning is alternately performed in the + X direction and the −X direction, an image can be recorded line by line in the same manner as described above.

(Step SA-2 in FIG. 2)
On the other hand, simultaneously with the scanning by the first scanning unit 11, the second scanning unit 12 also acquires the second image while scanning the object 1 at the same position and direction as the first scanning unit 11. Here, the scanning speed of the second scanning unit 12 is slower than that of the first scanning unit 11 (that is, sufficiently slower than the expected lifetime of phosphorescence). For this reason, in the second scanning unit 12, adjacent pixel positions can not be scanned until the luminescence of phosphorescence is attenuated, and as a result, phosphorescence at one point is observed as a mere point. Therefore, the second image obtained by the second scanning unit 12 of the present embodiment does not have a blur that reflects the lifetime of phosphorescence (see FIG. 3). The obtained second image is recorded by the recording unit 13 in the same manner as the first image.

  Here, in the present embodiment, since the second image is acquired while scanning the object 1 in the second scanning unit 12 simultaneously with the scanning by the first scanning unit 11, for example, the object (sample) is Even when moved, there is an advantage that it becomes easy to acquire two images at the same position. However, when the movement of the object is small or when a certain amount of error is acceptable, it is not always necessary to scan at the same time. Further, the acquisition timing of the first image and the second image does not have to be the same, and a slight deviation is allowed, and for example, alternate acquisition is possible. In short, if it is possible to calculate a shake function having a degree of accuracy that is practically acceptable, it is possible to set various image acquisition times.

(Step SA-3 in FIG. 2)
Next, the image dividing unit 21 of the analyzing unit 20 divides the first image and the second image into a plurality of regions. An image obtained by being divided is referred to as a divided image in this specification. An example of a divided image generated from the first image or the second image is schematically shown in FIG. In FIG. 5, the divided areas are indicated by reference numerals 61 to 6 n.

(Step SA-4 in FIG. 2)
Next, the shake function calculation unit 22 calculates a shake function in each divided image using divided images (corresponding to the same position) obtained from the first image and the second image described above. Hereinafter, a specific example of the blur function calculation procedure will be described with reference to FIG. In FIG. 6, the scanning direction (that is, the blur direction in the image) is the x direction.

  The phosphorescence intensity distribution g (x) on the x-axis in the first image can be expressed by the following formula.

As can be seen from the above, since g (x) and f (x) are known from the first and second images, these images can be used to determine h _j (x) at each point of the image it can. Therefore, in the present embodiment, h _j (x) in the first image is obtained using g (x) obtained from the first image and f (x) (corresponding to an example of the image information) obtained from the second image. This corresponds to calculating (corresponding to an example of the blurring function). In the present embodiment, h _j (x) in the first image is obtained in units of divided images.

(Step SA-5 in FIG. 2)
Next, the lifetime calculation unit 23 calculates the phosphorescence lifetime of the probe in the region using the obtained h _j (x). This calculation can be performed by obtaining a correspondence relationship (for example, a calibration curve) between the phosphorescence lifetime and h _j (x) in advance through experiments or the like.

(Step SA-6 in FIG. 2)
Next, the oxygen partial pressure calculation unit can calculate the oxygen partial pressure in the region using the obtained phosphorescence lifetime. Since the relationship between the phosphorescence lifetime and the oxygen partial pressure is known (see, for example, Non-Patent Document 1), the oxygen partial pressure can be calculated by using the relationship.

(Step SA-7 in FIG. 2)
On the other hand, the lifespan image generation unit 40 changes the color or texture of the divided area according to the obtained lifespan, and superimposes it on the first image or the second image. As a result, a life image in which the life or oxygen partial pressure distribution can be understood at a glance can be generated.

  Next, the presenting unit 50 presents the obtained life image to the user.

  In the measurement apparatus of the present embodiment, the phosphorescence life can be easily calculated by software-like image processing using an apparatus configuration of a so-called two-photon microscope. Therefore, according to this device, since it is not necessary to use special equipment such as a photon counter, there is an advantage that cost reduction of the device can be achieved.

  Further, in the measurement apparatus of the present embodiment, photoluminescence (phosphorescence or fluorescence) lifetime measurement is performed in software (that is, by computer calculation) based on a blur function calculated from an image obtained by scanning. Therefore, there is also an advantage that this measurement operation can be performed quickly. Of course, it is not essential to implement the calculation procedure by a computer program. For example, the above-described calculation can be performed using hardware such as ASIC or using hardware and a program together.

  Furthermore, according to the measurement apparatus of the present embodiment, since the apparatus configuration of a so-called two-photon microscope is used, the fluorescence images obtained by administering the fluorescent probe to the sample are the first and second images obtained by phosphorescence as described above. It is possible to shoot at the same time (so-called multi-color shooting). Then, there is also an advantage that the two-photon biological tissue construction and the oxygen partial pressure image can be displayed in an overlapping manner.

(Specific example of first and second images)
FIG. 7 shows an example of the second image with less blurring . FIG. 8 shows an example of a first image that is blurred with respect to the second image. In these drawings, an example of scanning in the horizontal direction in the drawings is shown.

  FIG. 9 shows an example in which the divided region is generated by dividing the image of FIG. FIG. 10 shows an example in which the divided region is generated by dividing the image of FIG.

  The contents of the present invention are not limited to the above embodiments. In the present invention, various modifications can be made to the specific configuration within the scope of the claims.

  For example, in the embodiment described above, the shake function is calculated for each divided area, but it is possible to calculate the shake function for each image.

  In the above-described embodiment, a resonant scanner is used as the first scanning unit 11 and a galvano scanner is used as the second scanning unit 12. However, the present invention is not limited to this, and a difference in scanning speed sufficient to calculate a blur function is obtained. You can use any scanner you have. For example, a high speed type galvano scanner can be used as the first scanning unit 11, and a low speed type galvano scanner can be used as the second scanning unit 12. Furthermore, it is also possible to use one scanner capable of changing the scanning speed as the first scanning unit for high-speed operation and as the second scanning unit for low-speed operation. Instead of the scanners described above, a polygon mirror or an acoustooptic device can be used as the first or second scanning unit.

  In the above-described embodiment, the blur function or the degree of deformation thereof is calculated using the first image and the second image. However, when the second image is obtained in advance by learning or the like, only the acquisition of the first image is performed. It is considered that the blur function can be calculated with

  Furthermore, two types of probes having different emission wavelengths and lifetimes can be applied to the object, and the respective emission wavelengths can be detected simultaneously by the first scanning unit and the second scanning unit. In this way, images corresponding to the respective emission wavelengths can be acquired as the first image and the second image. In this case, blurring occurs in the image corresponding to the long wavelength of the light emission life, and blurring does not occur in the image for the short wavelength of the light emission life, so the blur function can be calculated in the same manner as described above.

  Or in the said embodiment, a 2nd scanning part can also be set as the structure which acquires an image via a pinhole (an example of a blurring prevention means) used with a confocal microscope. In this way, even if the first scanning unit and the second scanning unit have the same scanning speed, a second image with less blur can be acquired. In this way, there is an advantage that mounting as a device becomes easy.

  Furthermore, in the above-described embodiment, phosphorescence is assumed as photoluminescence. However, if the scanning speed in the first scanning unit is sufficiently high, it is possible to measure the fluorescence lifetime based on the same principle as described above. is there.

  In the embodiment described above, the first image and the second image are acquired via the half mirror 14, but the installation of the half mirror is not essential, and the image is acquired via an independent optical path. There may be. In short, there are no particular restrictions on the optical configuration for acquiring the first image and the second image.

  Furthermore, in FIG. 1, although the 1st scanning part 11 and the 2nd scanning part 12 are described as an independent element, it is not the meaning which requires two scanning parts as a mechanical structure. For example, appropriate scanning means such as one galvano mirror or polygon mirror can be shared as the first scanning unit and the second scanning unit. When the acquisition timings of the first image and the second image do not have to be simultaneous, it becomes easy to share the same scanning means.

  In the above-described embodiment, the image sampling interval for acquiring the first image and the second image is set to be longer than the image sampling interval at which the blur due to the point spread function (PSF) can be observed (that is, the image resolution is increased). (Lower). PSF is a function representing blur determined by the wave nature of light, and is observed when the image sampling interval is somewhat short. If the acquired image is affected by blur due to PSF, the accuracy of the blurring function calculated using the first and second images may deteriorate, and the phosphorescence lifetime estimation accuracy may deteriorate. In the case of performing processing for removing PSF by calculation, there is a problem that the number of calculation processing steps increases. On the other hand, by increasing the image sampling interval of the first image and the second image to such an extent that blur due to PSF is not observed (however, it is shortened so that blur due to phosphorescence lifetime can be observed). There is an advantage that the influence can be removed and the phosphorescence lifetime estimation accuracy can be improved. For the second image that is not affected by the blur function, the effect of PSF can be removed by performing image processing for reducing the resolution of the image obtained at a short sampling interval. However, it is preferable to set the sampling interval at the time of acquiring the second image to be as long as that of the first image because this can reduce the number of image processing steps.

  Each of the above-described components only needs to exist as a functional block, and does not have to exist as independent hardware. As a mounting method, hardware or computer software may be used. Furthermore, one functional element in the present invention may be realized by a set of a plurality of functional elements, and a plurality of functional elements in the present invention may be realized by one functional element.

  Furthermore, the functional elements may be arranged at physically separated positions. In this case, the functional elements may be connected by a network. It is also possible to realize functions or configure functional elements by grid computing or cloud computing.

DESCRIPTION OF SYMBOLS 1 object 3 point image 4 blurred image 10 image acquisition part 11 1st scanning part 12 2nd scanning part 13 recording part 14 half mirror 20 analysis part 21 image division part 22 calculation part 23 lifetime calculation part 24 oxygen partial pressure calculation part 30 Multi-photon excitation unit 40 Lifetime image generation unit 50 Presentation unit 61-6n Divided region

Claims (11)

An apparatus for measuring the lifetime of photoluminescence in an object, comprising
It has an image acquisition unit and an analysis unit,
The image acquisition unit includes a first scanning unit and a recording unit.
The first scanning unit is configured to acquire a first image of the object by scanning the object in at least one direction.
Further, the scanning speed of the first scanning unit is set to be high enough to acquire a first image having a blur extending in the one direction from the light emitting point by the photoluminescence.
The recording unit is configured to record the acquired first image,
The analysis unit is configured to calculate a life of photoluminescence in the object by calculating a shake function in the one direction using the first image having the shake. measuring device. Furthermore, a second scanning unit is provided,
The second scanning unit is configured to acquire a second image of the object by scanning in the same direction as the scanning direction by the first scanning unit,
The scanning speed in the second scanning unit is set to a scanning speed slow enough that the second image does not have a blur extending in one direction from the light emitting point by the photoluminescence .
The recording unit is configured to record the acquired second image,
The photoluminescence lifetime measuring apparatus according to claim 1, wherein the analysis unit is configured to further use image information in the second image in order to calculate the lifetime of the photoluminescence in the object. Furthermore, it has a multiphoton excitation unit,
The photoluminescence lifetime measuring apparatus according to claim 1, wherein the multiphoton excitation unit is configured to cause light emission by a multiphoton absorption process to a probe disposed in the object. The photoluminescence lifetime measuring apparatus according to any one of claims 1 to 3, wherein the first scanning unit is configured using a resonant scanner capable of scanning in at least the one direction. The photoluminescence lifetime measuring apparatus according to claim 2 , wherein the second scanning unit is configured using a galvano scanner capable of scanning in at least the one direction. The photoluminescence lifetime according to any one of claims 1 to 5, wherein the analysis unit is configured to calculate a shake function in the one direction with respect to a divided image obtained by dividing the first image. measuring device. Furthermore, it has a lifetime image generation unit and a presentation unit,
The lifetime image generation unit is configured to generate a lifetime image representing the lifetime calculated by the analysis unit,
The photoluminescence lifetime measuring apparatus according to any one of claims 1 to 6, wherein the presentation unit is configured to present the lifetime image to a user. Furthermore, a second scanning unit is provided,
The second scanning unit is configured to acquire a second image of the object by scanning in the same direction as the scanning direction by the first scanning unit,
The second scanning unit includes an anti-blur unit for preventing the blur of the second image,
The recording unit is configured to record the acquired second image,
The photoluminescence lifetime measuring apparatus according to claim 1, wherein the analysis unit is configured to further use image information in the second image in order to calculate the lifetime of the photoluminescence in the object. The photoluminescence according to any one of claims 1 to 8, wherein an image sampling interval for acquiring the first image is set longer than an image sampling interval capable of observing blurring due to a point spread function (PSF). Sense life measuring device. Using the photoluminescence lifetime measuring apparatus according to any one of claims 1 to 9,
The first scanning unit acquiring a first image of the object by scanning the object in at least one direction;
Recording the acquired first image by the recording unit;
Calculating the life of the photoluminescence in the object by calculating the shake function in the one direction for the first image by the analysis unit. It comprises the photoluminescence lifetime measuring device according to any one of claims 1 to 9, and an oxygen partial pressure calculation unit,
The oxygen partial pressure calculation unit is configured to calculate an oxygen partial pressure in the object using the calculated lifetime of the photoluminescence.