JP6567808B2 - Cleaning composition - Google Patents

Description

  The present invention relates to a cleaning composition.

  Dry skin is a symptom that is commonly seen in dry seasons. On the other hand, many people suffer from chronic dry skin throughout the year. In healthy skin, intercellular lipids such as cholesterol and ceramide are considered to play an important role in the barrier function of the stratum corneum. Chronic dry skin is in a state where the water retention capacity of the keratin is reduced due to a decrease in intercellular lipids such as cholesterol and ceramide.

JP 2000-265191 A JP 2003-238390 A JP 2003-306426 A JP-A-11-92785

  In order for people with dry skin not to exacerbate their symptoms, moisturize with a moisturizing agent that maintains keratinous water, and do not further reduce intercellular lipids such as cholesterol and ceramide in the keratin during washing. Is also necessary. Currently, there are many cleansing agents for dry skin such as face wash and body wash.

  However, there are individual differences in the symptoms of dry skin, and it can not be said that there are still sufficient cleansing agents on the market that are particularly satisfactory for those who have chronic dry skin symptoms. An ideal cleansing agent for chronic dry skin is to retain intercellular lipid components (cholesterol, ceramide, etc.) necessary for the barrier function of the stratum corneum while removing unnecessary sebum soil components. It is considered that it has a selective cleaning property. Some cleaning agents having selective cleaning properties have been proposed so far (for example, Patent Documents 1 to 4). However, there are few cleaning agents that have a selective cleaning effect on commercialized products, and the selective cleaning effect is not satisfactory.

  An object of the present invention is to provide a cleaning agent having selective detergency capable of efficiently removing sebum dirt unnecessary for the skin and suppressing the elution of intercellular lipids necessary for the keratin barrier function. And

  The present inventors use a combination of an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, and blend these surfactants in a specific ratio to achieve high selective detergency. It has been found that a cleaning agent having is obtained.

  That is, the present invention is a cleaning composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, the anionic surfactant and the nonionic surfactant. In a mass ratio of 2/1 to 1/3, and the amphoteric surfactant is a mass of 1/4 or less with respect to the total amount of the anionic surfactant and the nonionic surfactant. A cleaning composition containing a ratio is provided.

  Cholesterol, ceramides, etc. that effectively remove sebum such as squalene, which is unnecessary dirt on the skin, and play an important role for the keratin barrier function by washing the skin with the above-mentioned cleaning composition Elution of intercellular lipids can be suppressed.

  The anionic surfactant is one selected from the group consisting of cocoyl methyl taurine sodium taurine, N-coconut oil fatty acid acyl-L-glutamic acid sodium, caproyl methyl taurine sodium and N-coconut oil fatty acid acylglycine sodium. It is preferable to include the above.

  The nonionic surfactant preferably contains a polyoxyethylene sorbitan monolaurate having an average added mole number of ethylene oxide of 10 to 100.

  The amphoteric surfactant is selected from the group consisting of lauryl hydroxysulfobetaine, amidopropyl betaine laurate, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and coconut oil fatty acid amidopropyl betaine. It is preferable to include two or more.

  The detergent composition may further contain at least one selected from the group consisting of pH adjusters, polyhydric alcohols, humectants, cholesterols, phytosterols, ceramides, and cationic polymers.

  The present invention also relates to a cleaning composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, wherein the anionic surfactant is an amino acid-based anionic interface. The nonionic surfactant contains a polyoxyethylene sorbitan monolaurate having an average added mole number of ethylene oxide of 10 to 100, and the amphoteric surfactant is a betaine amphoteric surfactant. And a selective detergent composition having a leakage rate of the fluorescent dye from the fluorescent dye-encapsulated liposome of 30% or less.

  The present invention also relates to a cleaning composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, wherein the anionic surfactant is an amino acid-based anionic interface. The nonionic surfactant contains a polyoxyethylene sorbitan monolaurate having an average added mole number of ethylene oxide of 10 to 100, and the amphoteric surfactant is a betaine amphoteric surfactant. A detergent composition that suppresses elution of intercellular lipids is provided.

  By washing the skin using the cleaning composition of the present invention, it is possible to remove sebum dirt such as squalene unnecessary for the skin and to suppress elution of intercellular lipids necessary for the keratin barrier function. .

6 is a graph showing a ratio of squalene concentration / cholesterol concentration in Test Example 3.

  Hereinafter, the present invention will be described in detail.

  The cleaning composition of the present invention contains an anionic surfactant, a nonionic surfactant and an amphoteric surfactant.

(Anionic surfactant)
The anionic surfactant used for the said detergent composition is not specifically limited, A well-known thing can be used. Among the anionic surfactants, amino acid-based anionic surfactants are preferably used. Examples of the amino acid-based anionic surfactant include cocoyl methyl taurine taurine sodium, cocoyl methyl taurine sodium, cocoyl methyl taurine potassium, cocoyl methyl taurine magnesium, N-coconut oil fatty acid sodium acyl-L-glutamate, potassium cocoyl glutamate, Cocoylglutamate triethanolamine, caproylmethyltaurine sodium, N-coconut oil fatty acid acylglycine sodium, cocoylglycine potassium, cocoylglycine triethanolamine, lauroylglutamate sodium, lauroylmethyl-β-alanine sodium, cocoylsarcosine sodium, cocoylalanine tri Ethanolamine, sodium stearoyl glutamate, sodium myristoyl glutamate Um, sodium cocoyl isethionate, and the like.

  Among the amino acid anionic surfactants, those having lower cytotoxicity are preferably used. Examples of amino acid-based anionic surfactants having low cytotoxicity include cocoyl methyl taurine taurine sodium, N-coconut oil fatty acid acyl-L-glutamic acid sodium, caproyl methyl taurine sodium, N-coconut oil fatty acid acylglycine sodium, Examples include sodium lauroyl aspartate, sodium myristoyl glutamate, sodium lauroyl glutamate, potassium cocoyl glutamate, and the like. Among these, cocoyl methyl taurine sodium taurine, N-coconut oil fatty acid acyl-sodium L-glutamate, caproylmethyl taurine sodium or sodium N-coconut oil fatty acid acylglycine is more preferably used. An anionic surfactant may be used individually by 1 type, and may use 2 or more types together.

(Nonionic surfactant)
The nonionic surfactant used for the said cleaning composition is not specifically limited, A well-known thing can be used. Examples of nonionic surfactants include polyoxyethylene sorbitan fatty acid esters and polyglycerin fatty acid esters.

  Among the polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monoolein are selected from the viewpoints of maintaining a balance with anionic surfactants and amphoteric surfactants and improving selective cleaning properties. It is preferable to use acid esters or polyoxyethylene sorbitan monostearate. Polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monooleate, and polyoxyethylene sorbitan monostearate are sorbitan fatty acid esters that are partial esters of sorbitol or sorbitan and lauric acid, oleic acid, or stearic acid, respectively. In addition, ethylene oxide is added. Among these, it is more preferable to use polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monooleate or polyoxyethylene sorbitan monostearate having an average added mole number of ethylene oxide of 10 to 100. The average added mole number of ethylene oxide is preferably 20 to 90, and more preferably 40 to 80. Specifically, for example, polyethylene glycol-80 sorbitan laurate, polyethylene glycol-40 sorbitan oleate, polyethylene glycol-40 sorbitan stearate, or the like can be used.

  Polyglycerol fatty acid ester is an ester of polyglycerol obtained by polymerizing glycerol and fatty acid. Among the polyglyceryl fatty acid esters, polyglyceryl-2 distearate, polyglyceryl-2 diisostearate, polyglyceryl triisostearate are included among the polyglycerin fatty acid esters from the standpoint of maintaining a balance with anionic surfactants and amphoteric surfactants. -2, polyglyceryl-3 distearate, polyglyceryl-3 diisostearate, polyglyceryl-6 distearate, polyglyceryl-6 diisostearate, polyglyceryl-10 laurate, polyglyceryl-10 myristate, polyglyceryl-10 stearate, polyglyceryl-10 isostearate , Polyglyceryl-10 oleate, polyglyceryl-10 distearate, polyglyceryl-10 diisostearate, polyglyceryl tristearate-10 Polyglyceryl-10 trioleate, polyglyceryl-10 pentastearate, polyglyceryl-10 pentahydroxystearate, polyglyceryl-10 pentaisostearate, polyglyceryl-10 pentaoleate, polyglyceryl-10 heptearate, polyglyceryl-10 heptaoleate It is preferable to use polyglyceryl-10 decastearate, polyglyceryl-10 decaisostearate or polyglyceryl-10 dekaoleate. A nonionic surfactant may be used individually by 1 type and may use 2 or more types together.

(Amphoteric surfactant)
As an amphoteric surfactant used for the said cleaning composition, a well-known thing can be used without a restriction | limiting in particular. For example, lauryl hydroxysulfobetaine, lauric acid amidopropyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, N-lauroyl-N′-carboxymethyl-N′— Examples include sodium hydroxyethylethylenediamine, lauramidopropyl hydroxysultain, amide propyl dimethylamine oxide laurate, hydroxyalkyl (C12-14) hydroxyethyl sarcosine, coconut oil fatty acid amidopropyl betaine and the like. Among these, lauryl hydroxysulfobetaine, lauric acid amidopropyl betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine or coconut oil fatty acid amidopropyl betaine, which are betaine-based amphoteric surfactants, are used. Is preferred. One amphoteric surfactant may be used alone, or two or more amphoteric surfactants may be used in combination.

  The cleaning composition according to the present invention has a mass ratio of the anionic surfactant to the nonionic surfactant (hereinafter also referred to as “(a)”) of 2/1 to 1/3. It is a range.

  In the cleaning composition according to the present invention, the mass ratio of amphoteric surfactant / {anionic surfactant + nonionic surfactant} contained (hereinafter also referred to as “(b)”) is ¼. It is as follows. (B) is preferably 1/8 or less, more preferably 1/16 or less.

  The reason why the detergent composition has high selective detergency by including an anionic surfactant, a nonionic surfactant and an amphoteric surfactant in the specific mass ratio is estimated as follows. However, it is not limited to this theory. When any one type of surfactant is present in the aqueous solution, some molecules form micelles, but some molecules are free. Surfactant molecules present in a free state tend to adsorb and penetrate into the stratum corneum, destabilize the lamellar structure of intercellular lipids, and easily break the barrier function of the skin. On the other hand, when an anionic surfactant, a nonionic surfactant, and an amphoteric surfactant are present in an aqueous solution, these surfactants tend to form micelles. Decrease. Then, since the lamellar structure of the intercellular lipid can be further stabilized, it is considered that cholesterol necessary for the skin is hardly eluted. Further, it is considered that the increase in the internal volume of the micelle makes it easier to embrace the dirt and improves the ability to clean unnecessary sebum dirt.

  The content of the anionic surfactant may be any amount as long as (a) and (b) satisfy the above conditions as a balance with the nonionic surfactant and the amphoteric surfactant. It is 2-25 mass% in the whole thing, More preferably, it is 5-20 mass%. Further, the content of the nonionic surfactant may be an amount that satisfies the specific conditions (a) and (b) as a balance with the anionic surfactant and the amphoteric surfactant, preferably It is 1-20 mass% in cleaning agent composition whole quantity, More preferably, it is 3-15 mass%. Further, the content of the amphoteric surfactant may be an amount satisfying the condition (b) as a balance with the anionic surfactant and the nonionic surfactant, but preferably in the total amount of the detergent composition. It is 0.01-10 mass%, More preferably, it is 0.1-5 mass%.

  The total content of the anionic surfactant, nonionic surfactant and amphoteric surfactant is preferably 1 to 50% by mass, more preferably 5 to 30% by mass in the total amount of the detergent composition. is there. In addition, although the said total content is suitably set within the said range according to a use, a formulation form, etc., for example, if it is a liquid body wash, 10-20 mass% is preferable.

  In another embodiment of the present invention, a detergent composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, wherein the anionic surfactant is an amino acid anion. The nonionic surfactant contains an ionic surfactant, the polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100, and the amphoteric surfactant is betaine-based amphoteric A selective detergent composition comprising a surfactant and having a leakage rate of the fluorescent dye from the fluorescent dye-encapsulated liposome of 30% or less is provided.

  In the present embodiment, the amino acid anionic surfactant, the polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100 and the betaine amphoteric surfactant are the same as those described above. Can be used.

  More preferably, the detergent composition is a selective detergent composition in which the fluorescent dye leakage rate from the fluorescent dye-encapsulated liposome is 20% or less. The fluorescent dye leakage rate is, for example, that the total content of the surfactant and the liposome containing an intercellular lipid model composed of intercellular lipids such as ceramide, palmitic acid, and cholesterol, and encapsulating calcein as a fluorescent dye, for example, The leakage rate of calcein from the liposome can be determined by fluorescence measurement by reacting with a detergent composition of 3% by mass.

  In yet another embodiment of the present invention, a detergent composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, wherein the anionic surfactant is an amino acid type The anionic surfactant is included, and the nonionic surfactant includes a polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100, and the amphoteric surfactant is a betaine-based surfactant. Provided is a detergent composition that suppresses elution of intercellular lipids, which contains an amphoteric surfactant.

  In the present embodiment, the amino acid anionic surfactant, the polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100 and the betaine amphoteric surfactant are the same as those described above. Can be used.

  The detergent composition can suppress elution of intercellular lipids. For example, when the index of intercellular lipid is cholesterol and the index of sebaceous gland-derived lipid is squalene, the amount of squalene eluted from the application site and cholesterol when the detergent composition is applied to skin The ratio to the amount of elution (cholesterol elution amount / squalene elution amount) is preferably 2 or more, more preferably 3 or more, and still more preferably 4 or more on a mass basis.

(Other ingredients)
The detergent composition may contain additives such as a pH adjuster, a polyhydric alcohol, a humectant, cholesterols, phytosterols, ceramides, and a cationic polymer in addition to the surfactant.

  Examples of pH adjusters include inorganic acids (hydrochloric acid, sulfuric acid, phosphoric acid, polyphosphoric acid, boric acid, etc.), organic acids (lactic acid, sodium lactate, acetic acid, citric acid, sodium citrate, tartaric acid, malic acid, succinic acid). , Sodium succinate, oxalic acid, gluconic acid, fumaric acid, propionic acid, aspartic acid, epsilon aminocaproic acid, glutamic acid and aminoethylsulfonic acid), gluconolactone, ammonium acetate, inorganic base (sodium bicarbonate, sodium carbonate, Potassium hydroxide, sodium hydroxide, calcium hydroxide, and magnesium hydroxide) and organic bases (such as monoethanolamine, triethanolamine, diisopropanolamine, lysine, and triisopropanolamine).

  Examples of the polyhydric alcohol include glycerin, 1,3-butylene glycol, propylene glycol, polyethylene glycol and diglycerin trehalose.

  Examples of the humectant include lipids such as phospholipids (for example, lecithin and hydrogenated lecithin); plant extracts such as hamamelis extract, tea extract and perilla extract; hyaluronic acid, sodium hyaluronate, acetyl hyaluronic acid, sodium acetyl hyaluronate, Heparin analogues, chondroitin, mucopolysaccharides such as sodium chondroitin sulfate; polymer compounds such as collagen, elastin, keratin, chitin and chitosan; Alcohols; alanine, serine, leucine, isoleucine, threonine, glycine, proline, hydroxyproline, glucosamine, aspartic acid, theanine and arginine Which amino acids; natural moisturizing factors such as sodium lactate, urea and sodium pyrrolidonecarboxylate; sugar alcohols such as sorbitol; natural moisturizing factor derived components such as lactic acid, sodium pyrrolidonecarboxylate and urea; polyglutamic acid; MPC polymers (eg LIPIDURE ( A polymer having a phospholipid polar group such as (registered trademark); polyoxypropylene methylglucoside; trimethylglycine (betaine); hydroxyethylurea; acrylic acid / acrylamide / dimethyldiallylammonium chloride copolymer; Among these, lipids such as phospholipids; plant extract extracts such as hamamelis extract, tea extract and perilla extract are preferable in consideration of the feeling of use.

  Examples of cholesterols include cholesterol, stigmasterol, lanosterol, ergosterol and the like.

  Examples of phytosterols include phytosterol, phytosterol, and dehydrophytosterol.

As ceramides, for example, known ceramides can be used regardless of naturally derived products or synthetic products (including pseudo-ceramides). It is also possible to use commercially available products including synthetic products, semi-synthetic products, derivatives and composites. As such products, for example, “Ceramide 2”, “Ceramide 2” manufactured by Takasago International Inc. “Liposome”, “ceramide 2 vesicle” and “ceramide 2,5 vesicle”, “ceramide 3” and “ceramide 6 (II)” manufactured by EVONIC, and “phytopresome cera 2” and “manufactured by Nippon Seika Co., Ltd.” Phytosome sera 2, 3, 6 ". As pseudo-ceramide, although not ceramide itself, an ingredient having properties similar to ceramide has been developed. Pseudoceramides include, for example, “Eldeu PS306” manufactured by Ajinomoto Co., Inc., which is commercially available as dilauroyl glutamate (octyldodecyl / phytosteryl / behenyl), and Ajinomoto, which is commercially available as dilauroyl glutamate (phytosteryl / octyldodecyl). Examples thereof include “El Deu PS203” manufactured by Co., Ltd. and “Sofcare Ceramide SL-E” manufactured by Kao Corporation, which is commercially available as cetyl PG hydroxyethyl palmitamide.
In addition, sphingoid bases such as sphingosine and phytosphingosine, glycosphingolipids and sphingophospholipids are also known as ceramides, and commercially available raw materials can be used.

  Examples of the cationic polymer include polyquaternium-7, polyquaternium-10, polyquaternium-39, cationized cellulose, and hydroxypropyltrimonium chloride starch.

  The said cleaning composition may contain another component in the range which does not impair the effect of invention. Examples of other components include antioxidants, polysaccharides, amino acids, peptides, preservatives, fragrances, and coloring agents.

  Although the pH of the said cleaning composition is not specifically limited, It is 4.5-9.0, Preferably it is 5.0-8.0.

  The said cleaning composition can be used in order to wash | clean the sebum dirt of the skin of a face and a body. The above-mentioned detergent composition has selective detergency that can remove sebum soil unnecessary for the skin and retain intercellular lipids such as cholesterol and ceramide which are important for the keratin barrier function. Therefore, the above-mentioned cleaning composition hardly breaks the barrier function of the skin, and can be cleaned without deteriorating the symptoms of dry skin. The above-mentioned cleaning composition can be suitably used for cleaning chronic dry skin.

  The said detergent composition is excellent in foaming property. Therefore, sufficient foaming can be obtained by the user's hand without using a special instrument or the like for foaming the cleaning composition. The higher the foamability, the more efficiently the sebum dirt can be removed.

  The detergent composition can be used as a face wash, body wash, hand soap and the like. You may use the said cleaning composition foamed with instruments, such as a foam discharge type container.

  EXAMPLES Hereinafter, although an Example demonstrates this invention in detail, this invention is not limited by these.

(Test Example 1: Evaluation of cytotoxicity of amino acid surfactant)
Corneal epithelial cell line HCE-T (RIKEN BioResource Center) is seeded at a density of 1.0 × 10 ⁴ cells / well per well of a 96-well microtiter plate (Corning) at 37 ° C., 5% CO _2. The cells were cultured for 72 hours under the condition of humidity 90% RH. After confirming that the cells have been cultured to a confluent state, the medium is removed, and various amino acid-based anionic surfactants diluted with the medium so that the final concentration becomes 0.02% are added, and 1 is added at room temperature. Incubated for minutes. Thereafter, each test solution was completely removed. A live cell detection reagent CellCounting Kit-8 (manufactured by Dojindo) was added to each well in an amount of 100 μL, and cultured for 1 hour at 37 ° C., 5% CO ₂ and 90% humidity. Absorbance was measured using VersaMax (manufactured by Molecular Devices), and the amount of color developed in response to living cells was determined (measurement wavelength: 450 nm). Based on the actually measured values, the cell viability was calculated using the following formula, and the cytotoxicity of various amino acid surfactants was evaluated.
Cell viability (%) = 100 × (absorbance of each sample treated group) / (absorbance of untreated group)
In the following test, only an amino acid surfactant having a cell viability of 80% or more was used.

(Test Example 2: CL leakage rate evaluation using a pseudo intercellular lipid model)
After 392 mg of calcein was dispersed in 5 mL of purified water and adjusted to pH 7.5 using 5M NaOH, purified water was added to make a total volume of 10 mL to prepare a 63 mM calcein (CL) solution. Separately, 22 mg of ceramide (manufactured by Kao Corporation), 39 mg of palmitic acid (manufactured by Wako Pure Chemical Industries, Ltd.), 39 mg of cholesterol (manufactured by Wako Pure Chemical Industries, Ltd.) were weighed, and these were combined to give 5 mL of chloroform / methanol = It was dissolved at 2/1 (v / v). 2 mL of the obtained solution was collected in an eggplant flask, and the solvent was removed under reduced pressure to prepare a thin film. Furthermore, it was left under reduced pressure with a vacuum pump for 1 hour, and the solvent was completely removed to prepare a thin film of a lipid mixture comprising intercellular lipids. After adding 2 mL of CL solution to the eggplant flask in which the thin film was prepared, the thin film was peeled off using a vortex mixer while heating to 80 ° C. Subsequently, under a heating condition of 80 ° C., a CL type solution and a thin film mixture were sonicated for 20 minutes using a probe type sonicator, and then cooled to room temperature in a dark place. The mixture was filtered through a Sephadex G-50 Fine gel column (manufactured by GE Healthcare Japan) conditioned with Tris-HCl buffer (pH 7.5) to obtain a CL-encapsulated liposome fraction. 150 μL of a solution containing the CL-encapsulated liposome fraction was added to each well of a 96-well microtiter plate (Corning), and a single or mixed surfactant shown in Table 1 or Tris-HCl buffer (pH 7.5) as a control ) Was added at room temperature for 5 minutes, and the fluorescence intensity Fs (Ex / Em: 494/520 nm) was measured with a fluorescence plate reader. Thereafter, 20 μL of 10% Triton X-100 was added, and allowed to stand at room temperature for 5 minutes, and then fluorescence intensity Ff (Ex / Em: 494/520 nm) was measured with a fluorescence plate reader. The CL leakage rate was calculated using the following formula. A combination of surfactants that provided low irritation was studied.
CL leakage rate (%) = 100 × {(Ff−Fs in control treatment group) − (Ff−Fs in each sample treatment group)} / (Ff−Fs in control treatment group)

The blending ratio unit in Table 1 is mass%. Details of the components in the table are as follows.
・ Lauroyl glutamate sodium: Amisoft LS-11 (Ajinomoto Healthy Supply)
-Polyglyceryl monoisostearate: Decaglyn1-ISV (manufactured by Nikko Chemicals)
2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine: NIKKOL AM101 (Nikko Chemicals)

  According to the above evaluation, by using a combination of an anionic surfactant, a nonionic surfactant and an amphoteric surfactant, the CL leakage rate from the CL-encapsulated liposome, which is a pseudo-cellular lipid model, can be reduced. I understood.

(Test Example 3: Evaluation of sebum selective detergency in human skin elution test, and evaluation of correlation between CL leakage rate in simulated intercellular lipid model and sebum selective detergency in human skin elution test)
A 10% by mass detergent composition containing the same type of surfactant as used in Test Example 1 at a compounding ratio shown in Table 2 was prepared. A 20 mm caliber cup was fixed to the human forearm (n = 8, inside the forearm of men in their 20s to 30s), and 5 mL of a 10% by mass detergent composition was placed in the cup and capped. The cup was shaken at 160 rpm / min for 5 minutes to obtain a detergent composition containing an eluate from human skin as an eluate. The blending ratio unit in Table 2 is mass%.

  An arbitrary amount of ethyl acetate was added to the obtained eluate, and 1 M NaOH was further added to decompose the surfactant in the eluate. The ethyl acetate layer and the aqueous layer of the eluate were separated in a transparent state by centrifugation, and the ethyl acetate layer was recovered. Further, the same operation was repeated twice to collect the ethyl acetate layer, the solvent was removed with an evaporator, and 1 mL of chloroform was added to obtain an extract. This extract was analyzed under the following GC analysis conditions, and squalene and cholesterol concentrations (mass basis) were quantified. From the obtained concentration, the ratio of squalene concentration / cholesterol concentration in the extract was calculated. The results are shown in FIG.

GC analysis condition model: Agilent GC7890A
Column: Cross Linked 5% Phenyl Methyl Silicone 25m × 0.2mmφ × 0.33μm
Carrier gas: He, 1.0 ml / min, split ratio, 20: 1
Temperature conditions: 160 ° C., 1 min → temperature increase (20 ° C./min)→200° C., 1 min → temperature increase (10 ° C./min)→300° C., 10 min
Inlet temperature: 250 ° C., detector temperature: 300 ° C. (hydrogen flame ionization detector (FID))
Injection volume: 1 μl

  From the above evaluation, it is found that the ratio of squalene concentration / cholesterol concentration is increased by using a combination of an anionic surfactant, nonionic surfactant and amphoteric surfactant, that is, selective detergency is increased. It was. In addition, from Test Example 2 and Test Example 3, it is found that there is a correlation between the CL leakage rate from the pseudo-cellular lipid model evaluated in Test Example 2 and the sebum selective detergency in the human skin elution test. It was.

(Test Example 4: Foaming and selective detergency evaluation)
A cleaning composition containing each surfactant was prepared at a blending ratio shown in Table 3 (unit: mass%). Since it was found from the above-described Test Example 2 and Test Example 3 that there is a correlation between the CL leakage rate from the CL-encapsulated liposome and the sebum selective detergency, each formulation of the surfactant was used with the CL leakage rate as an index. The examples were evaluated for selective detergency. It can be said that the lower the CL leakage rate, the higher the selective cleaning performance. The CL leakage rate evaluation was performed in the same manner as in Test Example 2. Moreover, foaming property was also evaluated. As a foaming property evaluation method, 50 ml of the detergent composition of each formulation example was foamed for 2 seconds using a miller (“IFM-620DG” manufactured by Iwatani Corporation), and the foam in the Milcer container was left standing for 1 minute. The amount (volume) was measured. When the measured amount of foam was less than 120 ml, it was evaluated as “×”, when it was 120 ml or more and less than 240 ml, “Δ”, and when it was 240 ml or more, it was evaluated as “◯”. The results are shown in Table 4. The higher the foaming property, the stronger the sebum detergency.

The details of each surfactant in the table are as follows.
・ Cocoyl methyl taurine taurine sodium: Diapon K-TS (manufactured by NOF Corporation)
・ N-coconut oil fatty acid acyl-sodium L-glutamate: Amisoft ECS22-SB (Ajinomoto Healthy Supply)
Caproyl methyl taurine sodium: Diapon HF-SF (manufactured by NOF Corporation)
・ N-coconut oil fatty acid acylglycine sodium: Amilite GCS-12K (Ajinomoto Healthy Supply Co., Ltd.)
PEG-80 sorbitan laurate: Nonion LT-280 (manufactured by NOF Corporation)
・ Laurylhydroxysulfobetaine: Anhithol 20HD (Kao Corporation)
Lauric acid amidopropyl betaine: Levon 2000L (manufactured by Sanyo Chemical Industries)
2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine: Nissan Anon GLM-R-LV (manufactured by NOF Corporation)
Palm oil fatty acid amidopropyl betaine: Levon 2000HG (manufactured by Sanyo Chemical Industries)

  In the cleaning composition of the example, the foaming property was sufficiently high, and the CL leakage rate was sufficiently low. Therefore, it was found from the above evaluation that the cleaning composition of the example can be expected to have high selective detergency of efficiently removing sebum dirt and suppressing elution of intercellular lipids such as cholesterol.

Hereinafter, formulation examples of the present invention will be shown.
<Formulation formulation example 1: Foam discharge type face wash>
Cocoyl methyl taurine taurine sodium 5% by weight
PEG-80 sorbitan laurate 7% by weight
Lauryl hydroxysulfobetaine 2% by weight
Sodium hyaluronate 0.1% by weight
Dipotassium glycyrrhizinate 0.1% by weight
Cholesterol 0.2% by weight
Ceramide-2 0.01% by weight
Glycerin 5% by weight
Polyquaternium-7 0.1% by weight
Dibutylhydroxytoluene 0.05% by weight
Phenoxyethanol 0.3% by weight
EDTA-2Na 0.05 wt%
Citric acid appropriate amount perfume appropriate amount water balance
Total 100% by weight

<Prescription Formulation Example 2: Body Wash>
N-coconut oil fatty acid acyl-sodium L-glutamate 10% by weight
N-coconut oil fatty acid acylglycine sodium 6% by weight
PEG-40 sorbitan stearate 8% by weight
2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine 3% by weight
Sorbitol 5% by weight
Dioleic acid PEG-120 methyl glucose 1% by weight
Phytosterol 0.3% by weight
Ceramide-3 0.01% by weight
Polyquaternium-10 0.2% by weight
Dibutylhydroxytoluene 0.05% by weight
Propyl paraoxybenzoate 0.3% by weight
EDTA-2Na 0.05 wt%
Succinic acid appropriate amount sodium succinate appropriate amount perfume appropriate amount water balance
Total 100% by weight

<Prescription Formulation Example 3: Hand Wash>
Caproyl methyl taurine sodium 4% by weight
PEG-80 sorbitan laurate 2% by weight
Coconut oil fatty acid amidopropyl betaine 1% by weight
Cholesterol 0.05% by weight
Ceramide-1 0.01% by weight
BG 5% by weight
Alanine 0.2% by weight
Glycine 0.2% by weight
Cationized cellulose 0.1% by weight
Dibutylhydroxytoluene 0.05% by weight
Methyl paraoxybenzoate 0.2% by weight
Propyl paraoxybenzoate 0.1% by weight
EDTA-2Na 0.05 wt%
Lactic acid Appropriate amount Sodium lactate Appropriate amount Water Balance
Total 100% by weight

<Formulation formulation example 4: foam discharge type body wash>
N-coconut oil fatty acid acylglycine sodium 2% by weight
N-coconut oil fatty acid acyl-sodium L-glutamate 2% by weight
Cocoyl methyl taurine taurine sodium 1% by weight
PEG-80 sorbitan laurate 6% by weight
1.5% by weight of lauric acid amidopropyl betaine
Hamamelis extract 0.1% by weight
Hydroxyethylurea 0.1% by weight
Cholesterol 0.2% by weight
Ceramide-1 0.01% by weight
Ceramide-2 0.01% by weight
Ceramide-3 0.01% by weight
Glycerin 5% by weight
Polyquaternium-7 0.1% by weight
Dibutylhydroxytoluene 0.1% by weight
EDTA-2Na 0.05 wt%
Citric acid appropriate amount Sodium citrate appropriate amount Fragrance appropriate amount Water Balance
Total 100% by weight

Claims (8)

A detergent composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant,
Containing the anionic surfactant and the nonionic surfactant in a mass ratio of 2/1 to 1/3,
Containing the amphoteric surfactant in a mass ratio of 1/4 or less with respect to the total amount of the anionic surfactant and the nonionic surfactant;
The content of the anionic surfactant is 2 to 25% by mass in the total amount of the cleaning composition, and the content of the nonionic surfactant is 1 to 20% by mass in the total amount of the cleaning composition. Yes, the content of the amphoteric surfactant is 0.01 to 10% by mass in the total amount of the detergent composition,
The anionic surfactant is sodium cocoyl methyl taurine, sodium cocoyl methyl taurine, potassium cocoyl methyl taurine, magnesium cocoyl methyl taurate, N-coconut oil fatty acid acyl-L-sodium glutamate, potassium cocoyl glutamate, triethanol cocoyl glutamate Amine, sodium caproylmethyl taurate, N-coconut oil fatty acid acylglycine sodium, cocoylglycine potassium, cocoylglycine triethanolamine, sodium lauroylglutamate, sodium lauroylmethyl-β-alanine, cocoylalanine triethanolamine, sodium stearoylglutamate, myristoyl Sodium glutamate, sodium cocoyl isethionate, lauroyl And at least one selected from the group consisting of sodium aspartate,
A detergent composition comprising the nonionic surfactant comprising one or more selected from the group consisting of polyoxyethylene sorbitan monolaurate, polyoxyethylene sorbitan monooleate and polyoxyethylene sorbitan monostearate
(However,
Component (A) N-acyl acidic amino acid or salt thereof 2.5 to 15% by mass,
Component (B) Alkyl ether carboxylic acid represented by the following general formula (1) or a salt thereof 0.3 to 8% by mass,
R ¹ —O— (CH ₂ CH ₂ O) _n —CH ₂ —COOM (1)
(In the general formula (1), ^{R 1} represents an alkyl group having 4 to 22 carbon atoms, n represents a number of 0 to 20, M represents a hydrogen atom, an alkali metal, an alkaline earth metal or ammonium. )
Component (C) One or more amphoteric surfactants selected from the group consisting of alkylamidopropylbetaines having an alkyl group having 8 to 14 carbon atoms and alkylhydroxysulfobetaines having an alkyl group having 8 to 14 carbon atoms 0.2-5% by mass, and
Ingredient (D) water
The ratio of the total amount [(B) + (C)] of the component (B) and the component (C) to the component (A) is a mass ratio [ B) + (C)] / (A) × 100 = 15 to 120, and the ratio of the component (C) to the component (A) is (C) / (A) × 100 = 5 in mass ratio. Exclude the liquid detergent composition that is ~ 70. )   The anionic surfactant is one selected from the group consisting of cocoyl methyl taurine sodium taurine, N-coconut oil fatty acid acyl-L-glutamic acid sodium, caproyl methyl taurine sodium and N-coconut oil fatty acid acylglycine sodium. The cleaning composition of Claim 1 containing the above.   The said nonionic surfactant is a cleaning composition of Claim 1 or 2 containing the polyoxyethylene sorbitan monolauric acid ester whose average addition mole number of ethylene oxide is 10-100.   The amphoteric surfactant is selected from the group consisting of lauryl hydroxysulfobetaine, amidepropylbetaine laurate, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine and coconut oil fatty acid amidopropylbetaine. The cleaning composition according to any one of claims 1 to 3, comprising one or more.   Furthermore, at least 1 sort (s) selected from the group which consists of a pH adjuster, a polyhydric alcohol, a moisturizer, cholesterol, phytosterols, ceramides, and a cationic polymer is contained in any one of Claims 1-4. The cleaning composition as described. The total content of the anionic surfactant, the nonionic surfactant, and the amphoteric surfactant is 1 to 50% by mass in the total amount of the detergent composition. The cleaning composition according to item. A detergent composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant,
The anionic surfactant includes an amino acid anionic surfactant,
The nonionic surfactant includes polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100,
The amphoteric surfactant includes a betaine amphoteric surfactant,
The content of the anionic surfactant is 2 to 25% by mass in the total amount of the detergent composition,
Selective detergent composition wherein leakage rate of fluorescent dye from fluorescent dye-encapsulated liposome measured by the following method is 30% or less
(However,
Component (A) N-acyl acidic amino acid or salt thereof 2.5 to 15% by mass,
Component (B) Alkyl ether carboxylic acid represented by the following general formula (1) or a salt thereof 0.3 to 8% by mass,
R ¹ —O— (CH ₂ CH ₂ O) _n —CH ₂ —COOM (1)
(In the general formula (1), ^{R 1} represents an alkyl group having 4 to 22 carbon atoms, n represents a number of 0 to 20, M represents a hydrogen atom, an alkali metal, an alkaline earth metal or ammonium. )
Component (C) One or more amphoteric surfactants selected from the group consisting of alkylamidopropylbetaines having an alkyl group having 8 to 14 carbon atoms and alkylhydroxysulfobetaines having an alkyl group having 8 to 14 carbon atoms 0.2-5% by mass, and
Ingredient (D) water
The ratio of the total amount [(B) + (C)] of the component (B) and the component (C) to the component (A) is a mass ratio [ B) + (C)] / (A) × 100 = 15 to 120, and the ratio of the component (C) to the component (A) is (C) / (A) × 100 = 5 in mass ratio. Exclude the liquid detergent composition that is ~ 70. ).
Fluorescent dye leakage rate measuring method: liposome containing calcein as a fluorescent dye and having an intercellular lipid model and a detergent composition are reacted, and fluorescence intensity Ff (Ex / Em: 494/520 nm) is obtained using a fluorescent plate reader. Measure. As a control, the same measurement is carried out for those not containing a surfactant, and the leakage rate of calcein from the liposome is calculated by the following formula.
Leakage rate (%) = 100 × {(Ff−Fs in control treatment group) − (Ff−Fs in each sample treatment group)} / (Ff−Fs in control treatment group) A detergent composition comprising an anionic surfactant, a nonionic surfactant and an amphoteric surfactant,
The anionic surfactant includes an amino acid anionic surfactant,
The nonionic surfactant includes polyoxyethylene sorbitan monolaurate having an average addition mole number of ethylene oxide of 10 to 100,
The amphoteric surfactant includes a betaine amphoteric surfactant,
A detergent composition that suppresses elution of intercellular lipids (however, it is a foam aerosol composition ,
Component (A) N-acyl acidic amino acid or salt thereof 2.5 to 15% by mass,
Component (B) Alkyl ether carboxylic acid represented by the following general formula (1) or a salt thereof 0.3 to 8% by mass,
R ¹ —O— (CH ₂ CH ₂ O) _n —CH ₂ —COOM (1)
(In the general formula (1), ^{R 1} represents an alkyl group having 4 to 22 carbon atoms, n represents a number of 0 to 20, M represents a hydrogen atom, an alkali metal, an alkaline earth metal or ammonium. )
Component (C) One or more amphoteric surfactants selected from the group consisting of alkylamidopropylbetaines having an alkyl group having 8 to 14 carbon atoms and alkylhydroxysulfobetaines having an alkyl group having 8 to 14 carbon atoms 0.2-5% by mass, and
Ingredient (D) water
The ratio of the total amount [(B) + (C)] of the component (B) and the component (C) to the component (A) is a mass ratio [ B) + (C)] / (A) × 100 = 15 to 120, and the ratio of the component (C) to the component (A) is (C) / (A) × 100 = 5 in mass ratio. A liquid detergent composition of ~ 70;
(A) Polyoxyethylene sorbitan monofatty acid ester having an average added mole number of ethylene oxide of 60 to 100 mol
(B) Nonionic surfactant represented by the formula (I)
RCOX (I)
(Wherein RCO is an acyl group having 8 to 16 carbon atoms, X is a residue obtained by removing one hydrogen atom bonded to the nitrogen atom of one or more amine compounds selected from diethanolamine and / or N-methylethanolamine) Or a residue obtained by removing a hydrogen atom from one hydroxyl group of a disaccharide.)
(C) an acylamino acid type anionic surfactant having an acyl group having 8 to 14 carbon atoms, and
(D) Water
And a liquid detergent composition containing ).

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