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JP6630279B2 - Fried taste enhancer - Google Patents
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JP6630279B2 - Fried taste enhancer - Google Patents

Fried taste enhancer Download PDF

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JP6630279B2
JP6630279B2 JP2016550160A JP2016550160A JP6630279B2 JP 6630279 B2 JP6630279 B2 JP 6630279B2 JP 2016550160 A JP2016550160 A JP 2016550160A JP 2016550160 A JP2016550160 A JP 2016550160A JP 6630279 B2 JP6630279 B2 JP 6630279B2
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taurine
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JPWO2016047570A1 (en
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前田 竜郎
竜郎 前田
葉 廣瀬
葉 廣瀬
健二郎 下司
健二郎 下司
辰徳 西出
辰徳 西出
祐二 田上
祐二 田上
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Nisshin Foods Inc
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Description

本発明は、揚げ物の旨味や味の深みを増大させることができる、揚げ物の呈味増強剤及び揚げ物の製造方法に関する。   TECHNICAL FIELD The present invention relates to a flavor enhancer for fried food and a method for producing fried food, which can increase the umami and taste of the fried food.

揚げ物は、高温の油で加熱する調理法であり、加熱と同時に、食材表面の水分が油に置換されるため、食材の風味と油の風味と高温による焦げの風味が相俟って、独特の味わいが得られる食品である。他方、揚げ物は、調理後時間が経過したり、調理の仕方によっては油感の高いものができてしまい、風味に厚み、濃厚感が足りなくなったり、しつこくて食べにくくなったりすることがある。
そのため、食材の風味を活かし、揚げ物独特の風味も維持しながら、深い味わいを有する揚げ物が求められている。
Fried food is a cooking method of heating with high-temperature oil.At the same time as heating, the moisture on the surface of the food is replaced with oil, so the flavor of the food, the flavor of the oil, and the flavor of the burn due to the high temperature are combined, making it unique. It is a food that can be tasted. On the other hand, deep-fried foods may have a long time after cooking, or may have a high oily feeling depending on the manner of cooking, and may have a lack of thickness and richness in flavor, or may be persistent and difficult to eat.
Therefore, there is a demand for a deep-fried fried food while making use of the flavor of the food material and maintaining the unique flavor of the fried food.

特許文献1〜3には、カルノシンやアンセリンのようなイミダゾールジペプチド、タウリンのようなスルホン基含有化合物を用いるコク味付与剤が開示されている。また、特許文献4には、分子量1万以上のペプチド分画の比率が10%以上で、分子量2000以下の分画が大半を占める酵母分解エキスが、畜肉又は魚介類を煮出ししただし液について、だしの呈味を強化することが開示されている。また、特許文献5には、肉質蛋白質を分解した調味料が開示されている。しかしながら、これらの成分によっても、揚げ物特有の風味にコクのある厚み、濃厚感を与え、しつこさのない揚げ物を得るには不十分であった。   Patent Documents 1 to 3 disclose kokumi imparting agents using imidazole dipeptides such as carnosine and anserine and sulfone group-containing compounds such as taurine. Further, Patent Document 4 discloses that a yeast-decomposed extract in which the fraction of a peptide having a molecular weight of 10,000 or more is 10% or more and the fraction having a molecular weight of 2,000 or less occupies the meat or fish and shellfish, It is disclosed that the taste of the soup is enhanced. Patent Document 5 discloses a seasoning obtained by decomposing meaty proteins. However, even with these components, the flavor unique to the fried food is given a rich thickness and richness, and it is insufficient to obtain a fried food without persistence.

特開平7−203895号公報JP-A-7-203895 特開平7−265008号公報JP-A-7-265008 特開平8−289760号公報JP-A-8-289760 特開2005−102549号公報JP 2005-102549 A US2007077333(A1)US2007707333 (A1)

本発明は、食材の風味を活かし、揚げ物独特の風味も維持しながら、深い味わいを有する揚げ物を容易に得ることができる呈味増強剤等を提供することを課題とする。   It is an object of the present invention to provide a taste enhancer and the like that can easily obtain a deep-fried fried product while maintaining the unique flavor of the fried product while utilizing the flavor of the foodstuff.

上記の課題を達成するために、本発明者らは、種々の成分を揚げ物に適用し検討を重ねた。その結果、タウリンと動物性又は植物性蛋白の抽出物由来の特定の分子量のペプチドとの混合物が、揚げ物の風味に深みを与え、これを用いると味わい深い揚げ物食品が簡便に得られることを見出し、本発明を完成した。   In order to achieve the above object, the present inventors have applied various components to fried foods and made repeated studies. As a result, it has been found that a mixture of taurine and a peptide having a specific molecular weight derived from an animal or vegetable protein extract gives a deep flavor to the fried food, and that a fried food with a high taste can be easily obtained by using this. The present invention has been completed.

即ち本発明は、タウリン及び、動物性又は植物性蛋白の抽出物由来の分子量80万〜1万のペプチドの混合物を含有する揚げ物の呈味増強剤を提供する。
また本発明は、具材100質量部に対して、タウリン0.001〜0.5質量部、及び動物性又は植物性蛋白の抽出物由来の分子量が80万〜1万のペプチドの混合物0.001〜0.5質量部を付着させた後、油ちょうする揚げ物の製造方法を提供する。
That is, the present invention provides a taste enhancer for fried food containing a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from an extract of animal or vegetable protein.
In addition, the present invention provides a mixture of 0.001 to 0.5 parts by mass of taurine and a peptide mixture having a molecular weight of 800,000 to 10,000 derived from an extract of animal or plant protein, based on 100 parts by mass of ingredients. The present invention provides a method for producing fried frying after attaching 001 to 0.5 parts by mass.

図1は、鶏胸肉から得た抽出物のHPLCチャートを示す図である。FIG. 1 is a diagram showing an HPLC chart of an extract obtained from chicken breast. 図2は、鶏腿肉から得た抽出物のHPLCチャートを示す図である。FIG. 2 is a diagram showing an HPLC chart of an extract obtained from chicken thigh.

本発明の呈味増強剤及び揚げ物の製造方法で用いるタウリンは、牛の胆汁中から発見された成分であり、貝類、イカ、タコの肉や神経組織等に含まれている。タウリンは生物から抽出したものでも、合成したものでも使用することができるが、好ましくは抽出して得られたものである。タウリンは、市販品を利用してもよい。
本発明の呈味増強剤におけるタウリンは、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.3質量部になるよう配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、呈味増強剤10g中、タウリンの配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のタウリンの配合量(濃度)は好ましくは0.01〜5質量%である。また、呈味増強剤が、調味料や衣材である場合も同様に、呈味増強剤100質量%中のタウリンの配合量(濃度)は好ましくは0.01〜5質量%である。
本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、タウリンを0.001〜0.5質量部、好ましくは0.01〜0.3質量部付着させる。
Taurine used in the method for producing a taste enhancer and fried food of the present invention is a component found in bile of cattle, and is contained in shellfish, squid, octopus meat, nervous tissue, and the like. Taurine can be used either as extracted from an organism or as synthesized, but is preferably obtained by extraction. Taurine may use a commercial item.
The amount of taurine in the taste enhancer of the present invention is preferably 0.001 to 0.5 part by mass, more preferably 0.01 to 0.3 part by mass, based on 100 parts by mass of the ingredients of the fried food. Parts. For example, in a case where 10 g of a taste enhancer is adhered as powdered flour to 100 g of ingredients and a fried product is produced, the amount of taurine in 10 g of the flavor enhancer is preferably 0.001 to 0.5 g, and flour is powdered. The amount (concentration) of taurine in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass. Similarly, when the taste enhancer is a seasoning or a clothing material, the amount (concentration) of taurine in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass.
In the method for producing fried food of the present invention, 0.001 to 0.5 part by mass, preferably 0.01 to 0.3 part by mass of taurine is adhered to 100 parts by mass of ingredients of the fried product.

本発明の呈味増強剤及び揚げ物の製造方法で用いる動物性又は植物性蛋白の抽出物(抽出エキス)由来のペプチドは、牛、豚、鶏等の畜肉類;鰯、鮭等の魚類;イカ、タコ等の軟体動物類等の動物性蛋白から抽出して得られたペプチド、又は、大豆、麦、米等の穀類;ジャガイモ等のイモ類;ニンジン、ゴボウ等の根菜類等の植物性蛋白から抽出して得られたペプチドの内、ゲル濾過による分子量が80万〜1万ダルトンのペプチドである。動物性又は植物性蛋白由来であっても、加水分解を行ったものや、ゲル濾過による分子量が1万ダルトン未満のペプチドであると、ペプチド特有の苦味が強くなるため、本発明の効果は得られない。また、ゲル濾過による分子量が80万ダルトンを超えると、味が感じられにくくなる上、調製がしづらくなるため、コスト的にも不利になる。   Peptides derived from animal or vegetable protein extracts (extracted extracts) used in the method for producing a taste enhancer and fried food of the present invention include: meats such as cows, pigs, chickens; fishes such as sardines and salmon; Peptides extracted from animal proteins such as molluscs such as octopus, octopus, etc., or cereals such as soybean, wheat, rice; potatoes such as potatoes; and vegetable proteins such as root vegetables such as carrots and burdock Among the peptides obtained by extraction from E., it is a peptide having a molecular weight of 800,000 to 10,000 dalton by gel filtration. Even if it is derived from animal or vegetable protein, if it is hydrolyzed or a peptide having a molecular weight of less than 10,000 daltons by gel filtration, the effect of the present invention is obtained because the bitterness specific to the peptide becomes stronger. I can't. On the other hand, if the molecular weight by gel filtration exceeds 800,000 daltons, the taste becomes less likely to be felt and the preparation becomes more difficult, which is disadvantageous in terms of cost.

上記動物性又は植物性蛋白としては、動物又は植物における、蛋白質を多く含む部位を用いることが好ましく、例えば、動物性蛋白としては、筋肉、表皮等を、植物性蛋白としては、種、実等を用いることが好ましい。特に動物性蛋白が好ましく、筋肉繊維等の繊維状蛋白が最も好ましい。
原料となる動物性又は植物性蛋白からの抽出には、アルコール又は酸を用いて抽出する、公知の蛋白抽出方法を用いることができる。例えば、原料を細断、破砕等の方法で細分化し、50〜90%エタノール等のアルコール溶液、又は、10〜500mM酢酸等の酸溶液を用いて抽出することができる。こうして得られた蛋白抽出物から、目的物である分子量80万〜1万のペプチドを得る。また、動物性蛋白又は植物性蛋白としては、それぞれ、単一種類の動物又は植物のものを用いても良いし、複数種類の動物又は植物のものを組み合わせて用いても良い。また、動物性蛋白と植物性蛋白とを組み合わせて原料とすることもできる。
As the above-mentioned animal or plant protein, it is preferable to use a protein-rich site in an animal or a plant. For example, as the animal protein, muscle, epidermis, etc., and as the plant protein, seed, fruit, etc. It is preferable to use In particular, animal proteins are preferable, and fibrous proteins such as muscle fibers are most preferable.
For the extraction from animal or vegetable protein as a raw material, a known protein extraction method of extracting with an alcohol or an acid can be used. For example, the raw material can be minced by a method such as shredding or crushing, and extracted using an alcohol solution such as 50-90% ethanol or an acid solution such as 10-500 mM acetic acid. From the protein extract thus obtained, the target peptide having a molecular weight of 800,000 to 10,000 is obtained. In addition, as the animal protein or the plant protein, a single type of animal or plant may be used, or a plurality of types of animal or plant may be used in combination. Further, a raw material may be used in combination with an animal protein and a vegetable protein.

目的とする分子量のペプチドを得る方法は、ゲル濾過、限外濾過、遠心分離等の公知の方法によって、上記蛋白抽出物から分取する方法を用いることができるが、本発明における「ペプチドの混合物」には、分子量が80万〜1万(1万以上80万以下)のペプチドに加え、分取操作上、不可避的に混入する分子量80万超、1万未満の分画を含むことも、本願の効果を妨げない限り許容される。
本発明における「ペプチドの混合物」は、動物性又は植物性蛋白からの抽出物に由来するものであり、分子量が相互に異なるペプチドを含む混合物である。より好ましくは、ゲル濾過により分子量の分布を分析したときに、分子量が1万以上80万以下の範囲に複数のピークを有する混合物である。
「ペプチドの混合物」を構成するペプチドの分子量は、好ましくは50万〜5万、更に好ましくは50万〜10万である。
As a method for obtaining a peptide having a target molecular weight, a method of fractionating from the above protein extract by a known method such as gel filtration, ultrafiltration, or centrifugation can be used. Includes, in addition to peptides having a molecular weight of 800,000 to 10,000 (10,000 or more and 800,000 or less), a fraction having a molecular weight of more than 800,000 and less than 10,000, which is inevitably mixed in the preparative operation. Acceptable as long as the effects of the present application are not hindered.
The “mixture of peptides” in the present invention is derived from extracts from animal or plant proteins, and is a mixture containing peptides having mutually different molecular weights. More preferably, it is a mixture having a plurality of peaks in a molecular weight range of 10,000 to 800,000 when a molecular weight distribution is analyzed by gel filtration.
The molecular weight of the peptide constituting the “mixture of peptides” is preferably 500,000 to 50,000, more preferably 500,000 to 100,000.

本発明における「ペプチドの混合物」は、ゲル濾過法により決定される分子量において、分子量が80万〜1万のペプチドの割合が、全ペプチドの合計質量に対して80〜100質量%であることが好ましく、より好ましくは90〜100質量%、更に好ましくは100質量%である。同様に、本発明における「ペプチドの混合物」は、ゲル濾過法により決定される分子量において、分子量が50万〜5万(又は50万〜10万)のペプチドの割合が、全ペプチドの合計質量に対して80〜100質量%であることが好ましく、より好ましくは90〜100質量%、更に好ましくは100質量%である。
分子量を分析するためのゲル濾過HPLCの具体的な条件は実施例において後述する。
In the “mixture of peptides” in the present invention, the ratio of peptides having a molecular weight of 800,000 to 10,000 in the molecular weight determined by the gel filtration method may be 80 to 100% by mass relative to the total mass of all peptides. Preferably, it is 90 to 100% by mass, more preferably 100% by mass. Similarly, the “mixture of peptides” in the present invention is such that the ratio of peptides having a molecular weight of 500,000 to 50,000 (or 500,000 to 100,000) in the molecular weight determined by the gel filtration method is expressed by the total mass of all peptides. It is preferably 80 to 100% by mass, more preferably 90 to 100% by mass, and still more preferably 100% by mass.
Specific conditions of gel filtration HPLC for analyzing the molecular weight will be described later in Examples.

本発明の呈味増強剤におけるペプチドは、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.2質量部になるよう配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、呈味増強剤10g中、ペプチドの配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のペプチドの配合量(濃度)は好ましくは0.01〜5質量%である。
本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、ペプチドを0.001〜0.5質量部、好ましくは0.01〜0.2質量部付着させる。
The amount of the peptide in the taste enhancer of the present invention is preferably 0.001 to 0.5 part by mass, more preferably 0.01 to 0.2 part by mass, based on 100 parts by mass of the ingredients of the fried food. Parts. For example, in a case where 10 g of the taste enhancer is adhered to 100 g of the ingredients as a ground powder to produce fried food, the amount of the peptide in 10 g of the taste enhancer is preferably 0.001 to 0.5 g, and the ground powder is The amount (concentration) of the peptide in 100% by mass of the taste enhancer is preferably 0.01 to 5% by mass.
In the method for producing fried foods of the present invention, 0.001 to 0.5 parts by mass, preferably 0.01 to 0.2 parts by mass, of the peptide is attached to 100 parts by mass of ingredients for fried foods.

本発明の呈味増強剤におけるタウリンとペプチドの配合割合(質量比,前者:後者)、及び、本発明の揚げ物の製造方法によけるタウリンとペプチドとの使用割合(質量比,前者:後者)は、それぞれ、100:1〜1:100であることが好ましく、より好ましくは10:1〜1:10である。   Mixing ratio of taurine and peptide in the taste enhancer of the present invention (mass ratio, former: latter) and use ratio of taurine and peptide in the method for producing fried food of the present invention (mass ratio, former: latter) Is preferably from 100: 1 to 1: 100, and more preferably from 10: 1 to 1:10.

本発明の呈味増強剤は、上記の成分に加えて、酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩から選択される1種以上を配合すると、更に深い味わいが得られるようになるため好ましい。酸性アミノ酸は、側鎖にカルボキシル基等のプロトンを放出する基を有するアミノ酸であり、アスパラギン酸、グルタミン酸などが挙げられる。炭化水素アミノ酸は、側鎖に炭化水素基を有するアミノ酸であり、アラニン、バリン、ロイシン、イソロイシンなどが挙げられる。これらのアミノ酸(酸性アミノ酸、炭化水素アミノ酸、グリシン、又はそれらの塩)は、一種を単独で用いても良いし、2種以上を併用することもできる。以上の中でも、アスパラギン酸、グルタミン酸、アラニン、グリシンおよびその塩から選択される1種以上を配合するのが好ましい。   The taste enhancer of the present invention is preferably added with one or more selected from acidic amino acids, hydrocarbon amino acids, glycine, and salts thereof, in addition to the above components, because a deeper taste can be obtained. . The acidic amino acid is an amino acid having a group capable of releasing a proton such as a carboxyl group in a side chain, and examples thereof include aspartic acid and glutamic acid. The hydrocarbon amino acid is an amino acid having a hydrocarbon group in a side chain, and includes alanine, valine, leucine, isoleucine and the like. These amino acids (acidic amino acids, hydrocarbon amino acids, glycine, or salts thereof) may be used alone or in a combination of two or more. Among them, it is preferable to mix at least one selected from aspartic acid, glutamic acid, alanine, glycine and salts thereof.

本発明の呈味増強剤における上記アミノ酸は、その使用量が、揚げ物の具材100質量部に対して、好ましくは0.001〜0.5質量部、より好ましくは0.01〜0.2質量部となるように配合する。例えば、具材100gに呈味増強剤10gを打ち粉として付着させ、揚げ物を製造する場合、打ち粉である呈味増強剤10g中、アミノ酸の配合量は好ましくは0.001〜0.5gであり、打ち粉である呈味増強剤100質量%中のアミノ酸の配合量(濃度)は好ましくは0.01〜5質量%である。また、本発明の揚げ物の製造方法においては、揚げ物の具材100質量部に対して、アミノ酸ペプチドを0.001〜0.5質量部、好ましくは0.01〜0.2質量部付着させる。
本発明の呈味増強剤におけるタウリンとアミノ酸の配合割合(質量比,前者:後者)、及び、本発明の揚げ物の製造方法によけるタウリンとアミノ酸との使用割合(質量比,前者:後者)は、それぞれ、100:1〜1:100であることが好ましく、より好ましくは10:1〜1:10である。
The amount of the amino acid in the flavor enhancer of the present invention is preferably 0.001 to 0.5 part by mass, more preferably 0.01 to 0.2 part by mass, based on 100 parts by mass of the ingredients of the fried food. It is blended so as to be parts by mass. For example, when the flavor enhancer 10g is adhered to the ingredients 100g as ground powder to produce fried food, in 10g of the flavor enhancer that is ground powder, the amount of the amino acid is preferably 0.001 to 0.5g. The amount (concentration) of the amino acid in 100% by mass of the flavor enhancer, which is powdered flour, is preferably 0.01 to 5% by mass. In the method for producing fried food of the present invention, 0.001 to 0.5 part by mass, preferably 0.01 to 0.2 part by mass of the amino acid peptide is attached to 100 parts by mass of ingredients of the fried product.
Mixing ratio of taurine and amino acid in the taste enhancer of the present invention (mass ratio, former: latter) and use ratio of taurine and amino acid in the method for producing fried food of the present invention (mass ratio, former: latter) Is preferably from 100: 1 to 1: 100, and more preferably from 10: 1 to 1:10.

本発明の呈味増強剤は、前述したタウリン、ペプチド、アミノ酸に加えて、呈味増強剤を揚げ物食品に適用する形態に合わせて、適宜公知のその他成分を配合することができる。   In the taste enhancer of the present invention, in addition to the above-described taurine, peptide, and amino acid, other known components can be appropriately blended according to the form in which the taste enhancer is applied to deep-fried foods.

本発明の呈味増強剤を揚げ物用の調味料として用いる場合、水;油脂;酒;みりん;醤油、塩;砂糖;香辛料等、公知の調味料に配合される成分を配合して製造できる。本発明の呈味増強剤を揚げ物用の打ち粉として用いる場合、小麦粉等の穀粉;澱粉;糖類等、公知の打ち粉に配合される成分を配合して製造できる。本発明の呈味増強剤を揚げ物用の衣材として用いる場合、小麦粉等の穀粉;澱粉;糖類;卵粉;増粘剤;食塩、粉末醤油、発酵調味料やその他の調味料;香辛料;香料;ビタミン等の栄養成分;着色料;油脂;膨張剤等、公知の衣材に配合される成分を配合して製造できる。その他成分の配合量は、上記の本発明の呈味増強剤中のタウリン、ペプチド、アミノ酸量を配合できるように調整すればよいが、一般的には呈味増強剤100質量%中95〜99.9質量%程度である。   When the flavor enhancer of the present invention is used as a seasoning for fried foods, it can be produced by blending components that are blended with known seasonings such as water, oils and fats, sake, mirin, soy sauce, salt, sugar, and spices. When the flavor enhancer of the present invention is used as flour for frying, it can be produced by blending components known in flour such as flour such as flour; starch; and sugars. When the flavor enhancer of the present invention is used as a clothing material for fried foods, flour such as flour; starch; saccharides; egg powder; thickener; Nutrient components such as vitamins; coloring agents; fats and oils; swelling agents; and other components blended in known clothing materials. The amount of the other components may be adjusted so that the amount of taurine, peptide and amino acid in the above-mentioned taste enhancer of the present invention can be mixed, but generally, 95 to 99 in 100% by mass of the taste enhancer. About 0.9% by mass.

本発明の呈味増強剤を用いて揚げ物を製造する場合、上記の呈味増強剤の形態に応じて、通常の製造方法により行えばよい。例えば、揚げ物用の調味料の呈味増強剤を用いる場合、呈味増強剤をボウル等に張り、これに具材をくぐらせる等して付着させ、そのままか衣材を付着させて油ちょうする。また、揚げ物用の打ち粉の呈味増強剤を用いる場合、打ち粉を具材にまぶして付着させ、そのままか更に衣材を付着させて油ちょうする。また、揚げ物用の衣材の呈味増強剤を用いる場合、具材をそのままか必要に応じて調味液や打ち粉を付着させた後、衣材をまぶして付着させるか、衣液を水に溶かしたバッター液を付着させ、油ちょうすればよい。
揚げ物の具材としては、揚げ物とされる公知の各種の具材を特に制限なく用いることができ、例えば、牛、豚、鶏等の畜肉類、キス、エビ、イカ、タコ等の魚介類、イモ、ナス、カボチャ、ニンジン、タマネギ、キノコ類等の野菜類等が挙げられる。
When a fried product is produced using the taste enhancer of the present invention, it may be carried out by a usual production method according to the form of the taste enhancer. For example, when a flavor enhancer for a seasoning for fried food is used, the flavor enhancer is put on a bowl or the like, and the ingredients are passed through the bowl, etc., and then attached, or the clothing is attached as it is and the oil is fried. . In addition, when using a flavor enhancer of powdered flour for frying, the powdered flour is sprinkled on the ingredients and adhered to the ingredients, or the clothing is further adhered and fried. Also, when using a flavor enhancer for fried clothing, apply the seasoning liquid or powdered flour as it is or as necessary, then apply the clothing by spraying the clothing, or apply the clothing liquid to water. What is necessary is just to adhere the dissolved batter liquid and fry it.
As the ingredients of the fried food, various known ingredients that are fried can be used without particular limitation, for example, beef, pork, meat such as chicken, kiss, shrimp, squid, seafood such as octopus, Vegetables such as potatoes, eggplants, pumpkins, carrots, onions, mushrooms and the like can be mentioned.

本発明の呈味増強剤は、揚げ物用の公知の調味料、打ち粉又は衣材等に添加して用いる添加剤であっても良い。添加剤である呈味増強剤は、タウリン、分子量80万〜1万のペプチドの混合物のみからなるものや、タウリンと分子量80万〜1万のペプチドの混合物に更に前記のアミノ酸を配合したものであっても良い。また、本発明の揚げ物の製造方法においては、具材に対して、タウリンと分子量80万〜1万のペプチドの混合物と所望により配合する前記のアミノ酸を任意の順序で順次付着させても良いし、それらを含む混合物等として同時に付着させても良い。油ちょうは、油の温度を130℃以上に加熱して行うことが好ましく、より好ましくは160℃〜200℃に加熱して行う。   The flavor enhancer of the present invention may be an additive that is used by adding it to known seasonings for fried foods, powdered flour, clothes, and the like. The taste enhancer, which is an additive, is composed of only a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 or a mixture of taurine and a mixture of a peptide having a molecular weight of 800,000 to 10,000 which is further compounded with the amino acid. There may be. Further, in the method for producing fried food of the present invention, a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 and the aforementioned amino acid to be blended as desired may be sequentially adhered to the ingredients in any order. And a mixture containing them may be simultaneously attached. The frying is preferably performed by heating the oil to a temperature of 130 ° C. or higher, more preferably by heating to 160 ° C. to 200 ° C.

以下、実施例を挙げて、本発明を更に詳細に説明するが、本発明は以下の実施例より何ら限定されるものではない。   Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples.

(参考例1〜3)鶏由来のペプチドの製造
鶏胸肉100gをハサミで3mm角程度に細断し、更にホモジナイザーを用いて粉砕した。これに100mLの70%エタノールを加え、60分間振盪(60rpm)して抽出を行った。抽出操作後、0.45μm のフィルターで濾過して濾液を収集した。
この濾液のゲル濾過HPLCのチャートを図1に示す。このチャートの分子量80万〜1万の範囲を分取して参考例1とし、50万〜5万の範囲を分取して参考例2とし、80万超の範囲を分取して参考例3とした。
(Reference Examples 1 to 3) Production of Chicken-Derived Peptide 100 g of chicken breast was cut into pieces of about 3 mm square with scissors, and further ground using a homogenizer. To this, 100 mL of 70% ethanol was added, and the mixture was shaken (60 rpm) for 60 minutes to perform extraction. After the extraction operation, the solution was filtered through a 0.45 μm filter to collect a filtrate.
The gel filtration HPLC chart of this filtrate is shown in FIG. The range of the molecular weight of 800,000 to 10,000 is sampled as Reference Example 1, the range of 500,000 to 50,000 is sampled as Reference Example 2, and the range of more than 800,000 is sampled. It was set to 3.

上記のゲル濾過HPLCは下記条件にて行った。
カラムとしてTSK-GEL G3000SWXL+TSK-GEL G2000SWXL (東ソー社製)を使用した。移動相は、0.3MNaClを含むリン酸緩衝液20mM(pH7.0)を用いた。測定は25℃で行ない、流速0.5mL/分、検出器はDAD(ダイオードアレイ検出器)を用い280nmにおける吸光度を検出した。
また、参考例1〜3のペプチドの分取は、同条件のゲル濾過HPLCにおいて分子量マーカーにより作成した検量線から分子量画分を計算し、フラクションコレクターを用いて行った。各分子量画分のフラクションを回収した後、凍結乾燥機を用いて粉末にした。タンパク質の分子量マーカーとして、オリエンタル酵母工業(株)社製のグルタミン酸脱水素酵素(MW:290,000)、乳酸脱水素酵素(MW:142,000)、エノラーゼ(MW:67,000)、ミオキラーゼ(MW:32,000)、シトクロムC(MW:12,400)を用いた。
The gel filtration HPLC was performed under the following conditions.
As a column, TSK-GEL G3000SWXL + TSK-GEL G2000SWXL (manufactured by Tosoh Corporation) was used. The mobile phase used was 20 mM phosphate buffer (pH 7.0) containing 0.3 M NaCl. The measurement was performed at 25 ° C., the flow rate was 0.5 mL / min, and the absorbance at 280 nm was detected using a DAD (diode array detector) as a detector.
The peptides of Reference Examples 1 to 3 were fractionated by calculating the molecular weight fraction from a calibration curve prepared by using a molecular weight marker in gel filtration HPLC under the same conditions, and using a fraction collector. After collecting the fraction of each molecular weight fraction, it was made into a powder using a freeze dryer. Glutamate dehydrogenase (MW: 290,000), lactate dehydrogenase (MW: 142,000), enolase (MW: 67,000), myokylase (MW: 32,000), cytochrome manufactured by Oriental Yeast Co., Ltd. C (MW: 12,400) was used.

(参考例4〜6)鶏由来のペプチドの製造
参考例1と同様に、鶏腿肉200gを用いて抽出を行い、濾液を得た。この抽出物のゲル濾過HPLCのチャートを図2に示す。このチャートの分子量80万〜1万の範囲を分取して参考例4とし、50万〜10万の範囲を分取して参考例5とし、1万未満の範囲を分取して参考例6とした。
(Reference Examples 4 to 6) Production of Chicken-Derived Peptide In the same manner as in Reference Example 1, extraction was performed using 200 g of chicken thigh to obtain a filtrate. A gel filtration HPLC chart of this extract is shown in FIG. The range of the molecular weight of 800,000 to 10,000 of this chart is sampled as Reference Example 4, the range of 500,000 to 100,000 is sampled as Reference Example 5, and the range of less than 10,000 is sampled. 6.

(製造例1〜12)
表1に示す成分を均一に混合して、製造例1〜12のから揚げ用衣材をそれぞれ製造した。製造例1〜12の揚げ用衣材は、製造例1がタウリン、製造例7が分子量80万〜1万のペプチドを含有しない点で比較例であり、それら以外は実施例である。
(Production Examples 1 to 12)
The ingredients shown in Table 1 were uniformly mixed to produce the fried clothing materials of Production Examples 1 to 12, respectively. The fried clothing materials of Production Examples 1 to 12 are comparative examples in that Production Example 1 does not contain taurine and Production Example 7 does not contain a peptide having a molecular weight of 800,000 to 10,000, and the other examples are examples.

(評価試験1)
鶏腿肉の細片(25g/個)を20個ずつ用意し、製造例1〜12の各衣材を、肉100gあたりの衣材の付着量が10gになるよう計量して、肉全体にまぶしてよく付着させた。衣材の付着した鶏腿肉を、170℃に熱したサラダ油で4分間油ちょうして、鶏肉のから揚げを製造した。揚げたてのから揚げ(油からあげて5分後)、及び室温(約25℃)に1時間放置したから揚げの風味を、それぞれ10名のパネラーに、表2に示す評価基準で評価してもらった。その平均点を表1に示す。
(Evaluation test 1)
20 pieces of chicken thigh pieces (25 g / piece) were prepared, and the respective garments of Production Examples 1 to 12 were weighed so that the amount of the garment attached per 100 g of the meat was 10 g, and the whole meat was weighed. It was dusted and attached well. The chicken thigh to which the clothing material adhered was fried in salad oil heated to 170 ° C. for 4 minutes to produce fried chicken. The flavors of the freshly fried (five minutes after raising from the oil) and the fried food left for 1 hour at room temperature (about 25 ° C.) were evaluated by ten panelists according to the evaluation criteria shown in Table 2. . Table 1 shows the average points.

Figure 0006630279
Figure 0006630279

Figure 0006630279
Figure 0006630279

(製造例13〜20)
下記表3に示す配合で、製造例13〜20の揚げ物用衣材を製造した。製造例13〜20の揚げ用衣材は、製造例17〜20の揚げ用衣材が、製造例17〜19に含まれるペプチドの分子量が80万超である点及び製造例20に含まれるペプチドの分子量が1万未満である点で、それぞれ比較例であり、それら以外は実施例である。
(評価試験2)
各揚げ物用衣材100質量部に対して、水200質量部を混合してバッター液を製造した。打ち粉をした豚ロース肉100gに、このバッター液を絡め、更にパン粉を付着させ、170℃に熱したサラダ油で6分間油ちょうして、とんかつを製造した。
揚げたてのとんかつ(油からあげて5分後)、および室温(約25℃)に1時間放置したとんかつの風味を、それぞれ10名のパネラーに、表4に示す評価基準で評価してもらった。その平均点を表3に示す。
(Production Examples 13 to 20)
With the composition shown in Table 3 below, the fry clothing materials of Production Examples 13 to 20 were produced. The fried clothing materials of Production Examples 13 to 20 are the same as the fried clothing materials of Production Examples 17 to 20 except that the peptide contained in Production Examples 17 to 19 has a molecular weight of more than 800,000 and the peptide contained in Production Example 20 Are comparative examples in that the molecular weight is less than 10,000, and the others are examples.
(Evaluation test 2)
200 parts by mass of water was mixed with 100 parts by mass of each fry clothing material to produce a batter solution. This batter solution was entangled with 100 g of beaten pork loin meat, and bread crumbs were further adhered thereto. The mixture was fried with salad oil heated to 170 ° C. for 6 minutes to produce a tonkatsu.
The flavors of freshly fried tonkatsu (after 5 minutes from the oil) and tonkatsu left at room temperature (about 25 ° C.) for 1 hour were evaluated by ten panelists according to the evaluation criteria shown in Table 4. Table 3 shows the average scores.

Figure 0006630279
Figure 0006630279

Figure 0006630279
Figure 0006630279

(製造例21〜30)
下記表5の配合で、製造例21〜30のから揚げ用衣材を製造した。製造例21〜30のから揚げ用衣材は、何れも実施例である。
(評価試験3)
評価試験1と同様にして、製造例21〜28のから揚げ用衣材を用いて鶏肉のから揚げを製造した。揚げたてのから揚げ(油からあげて5分後)、および室温(約25℃)に1時間放置したから揚げの風味を、それぞれ10名のパネラーに、表2に示す評価基準で評価してもらった。その平均点を表5に示す。なお、表5には製造例4の結果を再掲する。
(Production Examples 21 to 30)
With the composition shown in Table 5 below, the fried clothing materials of Production Examples 21 to 30 were produced. The fried clothing materials of Production Examples 21 to 30 are all examples.
(Evaluation test 3)
In the same manner as in Evaluation Test 1, chicken fried chicken was produced using the fried clothing materials of Production Examples 21 to 28. The flavors of freshly fried (five minutes after raising from the oil) and fried for one hour at room temperature (about 25 ° C.) were evaluated by ten panelists according to the evaluation criteria shown in Table 2. . Table 5 shows the average scores. Table 5 shows the results of Production Example 4 again.

Figure 0006630279
Figure 0006630279

表1及び表3に示す結果から、タウリンと特定の分子量のペプチドの配合した実施例の呈味増強材によれば、食材の風味を活かし、揚げ物独特の風味も維持しながら、コク味及び総合的な風味を向上させることができることが判る。また、表5に示す結果から、酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩の一種以上を更に配合することにより、更に深い味わいが得られることが判る。   From the results shown in Tables 1 and 3, according to the taste enhancer of the example in which taurine and a peptide having a specific molecular weight are blended, the flavor of the ingredients is utilized, and while maintaining the unique flavor of fried food, the body taste and the overall taste are improved. It can be understood that the typical flavor can be improved. Further, from the results shown in Table 5, it is found that a further deep taste can be obtained by further blending one or more of acidic amino acids, hydrocarbon amino acids, glycine and salts thereof.

本発明の呈味増強剤は、揚げ物用の通常の調味料、打ち粉又は衣材等に添加したり、揚げ物用の通常の調味料、打ち粉又は衣材等として用いたりして、通常の方法で揚げ物を製造するだけで、コク味を有し、油のしつこさ等も感じさせにくい揚げ物を得ることができる。本発明の揚げ物の製造方法によれば、容易に、コク味を有し、油のしつこさ等も感じさせにくい揚げ物を得ることができる。コク味とは、味香りに厚みがあり、口中に広がった風味が長く続き、深い味わいを有することをいう。
The flavor enhancer of the present invention can be added to a normal seasoning for fried food, flour or a batter, or used as a normal seasoning for fried food, batter or a batter, etc. By simply producing the fried product by the method, it is possible to obtain a fried product that has a rich taste and does not easily feel the persistence of oil. ADVANTAGE OF THE INVENTION According to the manufacturing method of the deep-fried food of this invention, the deep-fried food which has a full-bodied taste and is hard to feel the persistence of oil, etc. can be easily obtained. The full-bodied taste means that the taste and aroma are thick, the flavor spread in the mouth lasts long, and has a deep taste.

Claims (7)

タウリン及び、動物の筋肉である動物性蛋白の抽出物由来の分子量80万〜1万のペプチドの混合物を含有する揚げ物の呈味増強剤 A taste enhancer for fried food containing a mixture of taurine and a peptide having a molecular weight of 800,000 to 10,000 derived from an extract of animal protein that is animal muscle . 前記ペプチドが、分子量50万〜5万のペプチドである、請求項1に記載の呈味増強剤。   The taste enhancer according to claim 1, wherein the peptide is a peptide having a molecular weight of 500,000 to 50,000. 更に酸性アミノ酸、炭化水素アミノ酸、グリシン及びこれらの塩から選択される1種以上を配合する、請求項1又は2に記載の呈味増強剤。   The taste enhancer according to claim 1 or 2, further comprising one or more selected from acidic amino acids, hydrocarbon amino acids, glycine, and salts thereof. 揚げ物用の調味料、打ち粉又は衣材である、請求項1〜3のいずれか1項に記載の呈味増強剤。   The flavor enhancer according to any one of claims 1 to 3, which is a seasoning, flour, or a batter for fried foods. 前記タウリンの配合量が0.01〜5質量%である、請求項4に記載の呈味増強剤。   The taste enhancer according to claim 4, wherein the amount of the taurine is 0.01 to 5% by mass. 前記ペプチドの配合量が0.01〜5質量%である、請求項4又は5に記載の呈味増強剤。 The taste enhancer according to claim 4 or 5 , wherein the amount of the peptide is 0.01 to 5% by mass. 具材100質量部に対して、タウリン0.001〜0.5質量部、及び動物の筋肉である動物性蛋白の抽出物由来の分子量が80万〜1万のペプチドの混合物0.001〜0.5質量部を付着させた後、油ちょうする揚げ物の製造方法。   0.001 to 0.5 parts by mass of taurine and a mixture of peptides having a molecular weight of 800,000 to 10,000 derived from an extract of animal protein, which is animal muscle, based on 100 parts by mass of ingredients. A method for producing fried food which is fried after attaching 5 parts by mass.
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