JP6654826B2 - Composition - Google Patents

Description

  The present invention relates to a composition comprising component (A) astaxanthin; component (B) Lemon balm plant of the family Labiatae, or extract thereof. Further, the present invention relates to an astaxanthin-degradation inhibitor and a degradation-suppressing method using a plant of the genus Lamiaceae, the genus Lemon balm, or an extract thereof.

Astaxanthins are known to have functions such as an antioxidant effect, an anti-inflammatory effect (see Patent Documents 1 and 2), a skin aging preventing effect (see Patent Document 3), and a whitening effect (Non-Patent Document 1). ing. For this reason, it has been studied and implemented to add astaxanthins to raw materials of foods, cosmetics, pharmaceuticals, and processed products thereof.
However, since astaxanthins are structurally unstable, it is difficult to incorporate them in a stable state in a preparation by photolysis or component oxidation.
In order to stabilize astaxanthins, some contrivances such as a combination of astaxanthins and ascorbic acid have been made (see Patent Document 4).
In addition, Patent Documents 5 to 7 relate to stabilization of carotenoid pigments.
Patent Document 5 discloses that a. An oil-soluble plant extract containing astaxanthin extracted from the petals of Adonis paraestina belonging to the family Ranunculaceae, Betula spp .; b. A dihydric alcohol having 4 to 8 carbon atoms; c. A polyphenol; d. An external preparation for skin containing a chelating agent is disclosed.
Patent Document 6 discloses that one or more of an organic acid having two or more carboxyl groups (excluding citric acid), glycine, and salts thereof contain a carotenoid pigment or a carotenoid pigment. There is disclosed a method for stabilizing a carotenoid pigment to be added to an object.
Patent Document 7 discloses the use of iron ions, an iron chelating agent, dipropylene glycol, and a water-soluble antioxidant in combination.

However, astaxanthins are unstable as described above, and it is difficult to stabilize astaxanthins because it is difficult to suppress the influence of light and the effects of other components. Was. Furthermore, when used in skin external preparations or cosmetics, the color of the astaxanthin compounded in the preparation is visible to the user, so that the discoloration change is a major problem in quality. Furthermore, if astaxanthins are decomposed and discolored, the antioxidant effect, anti-inflammatory effect, and skin aging prevention effect originally expected of astaxanthins cannot be sufficiently exhibited in the preparation.
For these reasons, it has been desired to stably blend astaxanthins which are known to have many useful effects.

JP-A-2-49091 Japanese Patent Application Laid-Open No. 9-143063 JP-A-5-155736 JP-A 1-215263 JP 2010-105988 A JP-A-6-264055 JP 2012-31067 A

Int. J. Mol. Sci. 2014 (15) 8293-8315

  The problem to be solved by the present invention is to provide a composition for improving the stability of astaxanthins, which are known to have useful effects such as whitening and anti-aging, and to provide a stabilizer and a stabilizing method. is there.

  In view of such circumstances, the present inventors have conducted intensive studies and, as a result, have found that astaxanthins can be stabilized by using a Lamiaceae plant of the genus Larvae and the extract thereof, to complete the present invention. Reached. By combining the astaxanthins with the lemon balm plant of the genus Lamiaceae or the extract of the mint, it is possible to exert a remarkable effect in preventing the fading of the astaxanthins by light. And, by stabilizing the astaxanthins suitably, the effects such as anti-aging effect and whitening effect of the astaxanthins contained in cosmetics and the like can be remarkably enhanced, and thereby such effects of the astaxanthins Can be provided.

The present invention provides a composition containing component (A) astaxanthins and component (B) Lemon balm plant of the genus Labiatae or its extract.
The present invention provides a cosmetic, an external preparation for skin, or a quasi-drug containing the composition.
The present invention provides the use of the composition for the manufacture of cosmetics, external preparations for the skin, quasi-drugs, pharmaceuticals, foods and drinks, or feeds.
The present invention provides a stabilizing agent for astaxanthin, which contains a Lamiaceae plant of the genus Lamiaceae, Lemon Balm plant or an extract thereof.

  ADVANTAGE OF THE INVENTION According to this invention, the composition which raises the stability of astaxanthin which is known to have useful effects, such as whitening and aging prevention, a stabilizer, and a stabilization method can be provided.

The astaxanthins of the component (A) in the present technology are one kind of carotenoids, and include astaxanthin and astaxanthin derivatives such as astaxanthin esters unless otherwise specified. The astaxanthins can be selected from astaxanthin and astaxanthin derivatives.
In general, astaxanthins are known to have an antioxidant effect, an anti-inflammatory effect, a skin aging preventing effect, a whitening effect, and the like. It is known that astaxanthin is widely distributed in nature and usually exists as an astaxanthin fatty acid ester, and also exists as an astaxanthin protein (oborbin, cluster cyanine) bound to a protein in a crustacean or the like. Usually, in the extraction of naturally-derived astaxanthin, the ratio of astaxanthin and astaxanthin fatty acid ester is increased so that the above-mentioned effects are suitably exerted, and the product is produced.

Furthermore, astaxanthin derivatives include, in addition to naturally occurring astaxanthin esters, appropriately synthesized astaxanthin salts, oxidized forms, reduced forms, geometric isomers, optical isomers, glycosides, ester forms, and the like.
More specific astaxanthin derivatives include, for example, amino acid esters such as glycine and alanine, carboxylic acid esters such as acetate ester and citrate ester and salts thereof; inorganic acid esters such as phosphate ester and sulfate ester and salts thereof; Glycosides such as glucoside; polyunsaturated fatty acids such as eicosapentaenoic acid and docosahexaenoic acid; unsaturated fatty acids such as oleic acid and linoleic acid; and fatty acid esters selected from saturated fatty acids such as palmitic acid and stearic acid. The monoester and the same or different diester are selected. Examples of the fatty acid include lower fatty acids having 2 to 4 carbon atoms, middle fatty acids having 5 to 12 carbon atoms, and higher fatty acids having 13 or more carbon atoms (for example, 16 to 22 carbon atoms).

Astaxanthin in a narrow sense is a red dye having an absorption maximum at 476 nm (ethanol) and 468 nm (hexane), and has a chemical structure of 3,3′-dihydroxy-β, β-carotene-4,4′-dione (C ₄₀ H). ₅₂ 0 _4, a molecular weight 596.82). This astaxanthin has 3S, 3S'-form, 3S, 3R'-form (meso-form), 3R, 3R'-form depending on the configuration of the hydroxyl group at the 3 (3 ')-position of the ring structure present at both ends of the molecule. There are three isomers of the body. Furthermore, there are cis- and trans- isomers of the conjugated double bond at the center of the molecule. For example, all cis-, 9-cis and 13-cis forms. In the present technology, any one can be used.

  Here, the hydroxyl group at the 3,3'-position can form an ester with a fatty acid. Astaxanthin from krill is a diester with two fatty acids combined, and the one from H. pluvialis is a 3S, 3S'-form with a high content of monoesters with one fatty acid combined . Astaxanthin obtained from red yeast (Phaffia Rhodozyma) has a 3R, 3R'-form and an opposite structure to the 3S, 3S'-form normally found in nature.

The component (A) astaxanthins of the present technology includes those extracted and purified from any of animals, plants, algae, bacteria and fungi. The product is not limited to those derived from nature, and any product (organic synthetic product) obtained according to a conventional method can be used. It is also possible to mix and use these as appropriate. In addition, astaxanthins of natural origin are derived from astaxanthins produced by living organisms.
Astaxanthins used in the present technology can be, for example, those extracted from natural products such as krill, salmon, trout, Fussou grass, red yeast, Haematococcus algae, and synthetic products. Also, for example, Katakura Chickalin Co., Ltd., Marine Daio Co., Ltd., Swiss Pharmaceuticals, Takeda Paper Co., Ltd., Fuji Chemical Industry Co., Ltd., Oriza Yuka Co., Ltd., Wako Pure Chemical Co., Ltd., Biogenic General products commercially available from companies and the like can be used.
Among them, those extracted from Haematococcus algae (hereinafter, also referred to as Haematococcus algae extract) are particularly preferable in terms of quality and productivity.
The extraction solvent for obtaining astaxanthins from natural products may be an aqueous solvent or an organic solvent.
As the organic solvent, alcohol (methanol, ethanol, isopropanol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, etc.), ethyl acetate, ether, hexane and the like can be used. In addition, carbon dioxide or the like in a supercritical state can also be used. These solvents may be used alone or as a mixture of two or more.

  As the origin of the Haematococcus alga extract that can be used in the present technology, specifically, Haematococcus pluvialis, Haematococcus lacustris, Haematococcus capensis, and Haematococcus capensis Haematococcus droebakensis, Haematococcus zimbabwiensis and the like. In addition, any commercially available Haematococcus alga extract can be used.

When astaxanthins are contained in the composition of the present technology, they may be blended using astaxanthin-containing products (for example, biological products). At this time, the content of astaxanthins in the product used as the component (A) (astaxanthin-free form) is not particularly limited, but is preferably 0.01 to 50% by mass (hereinafter simply referred to as “%”). It is more preferably 0.1 to 30%, particularly preferably 1 to 20%.
Further, the amount of astaxanthins in the composition of the present technology is preferably 0.000001 to 5%, more preferably 0.000001 to 0.5%, and still more preferably 0.0001 to 0.1%. %.
The dose or application amount of the component (A) astaxanthin is usually 0.001 to 10 mg (astaxanthin-free form), preferably 0.01 to 1 mg, and is divided into once to three times a day. It may be applied.

As the component (B) in the present technology, the scientific name of the plant of the genus Lamiaceae (Lamiaceae) genus (Melissa) lemon balm plant (hereinafter also referred to as “lemon balm plant”) is called (Melissa officinalis), and aliases such as melissa and mulberry , And also referred to as "Yamahakka". The lemon balm plant is widely used for flavoring food and drink.
In addition, the use site of the lemon balm plant, roots, rhizomes, stems, leaves, flowers, fruits, seeds and the like is not particularly limited, but it is preferable to use the above-ground parts in terms of astaxanthin stabilization, leaves are more suitable, It is preferable to use the plant as it is or after crushing it. Such a plant can be used as it is or as a juice obtained by squeezing it, a dried product obtained by drying the plant itself or a crushed product thereof, or an extract extracted therefrom. Is preferred.

The extraction method from the lemon balm plant is not particularly limited, and a general extraction method can be used.
The above-mentioned lemon balm extract is dried or cut without drying any one or more of whole plants, leaves, petals, seeds, roots and rhizomes, and then cut at a low temperature (for example, less than 4 ° C.), a normal temperature (for example, 4 ° C.). -40 ° C) and under heating (eg, 40-100 ° C) by extraction with a solvent. In addition, it is desirable to use 50 to 10000 mL of a solvent for 100 g of the lemon balm plant.

In addition, examples of the extraction solvent include water, an organic solvent, and the like.
Examples of the organic solvent include alcohols (monohydric, dihydric or trihydric or higher alcohols), esters (ethyl acetate, butyl acetate, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether). ), Hydrocarbons (hexane, benzene, pentane, etc.). In addition, carbon dioxide or the like in a supercritical state can also be used.
Examples of the monohydric alcohol include methanol, ethanol, isopropanol and the like. Examples of the dihydric alcohol include 1,3-butylene glycol, ethylene glycol, propylene glycol and the like. Examples of the trihydric alcohol include glycerin. Examples of the trivalent or higher alcohol include sugar alcohols such as sorbitol and maltitol, and polyethylene glycol. Of these, lower alcohols having 1 to 4 (preferably 2 to 3) carbon atoms are preferred.
From the viewpoint of use, water and / or a water-soluble organic solvent are preferable, and water, a lower alcohol (more preferably, ethanol, methanol, 1,3-butylene glycol or the like) or a mixed solvent thereof is more preferable. .
From the illustration of these extraction solvents, they may be used alone or as a mixture of two or more. Further, those extracted twice or more using different solvents may be mixed and used.

  As an example of a preferable extraction method, extraction is performed for 1 to 5 days using a lower alcohol (ethanol or 1,3-butylene glycol) having a solvent concentration of 0 to 100% by volume. The concentration of the organic solvent is preferably 30% by volume or more, more preferably 50 to 100% by volume. The temperature at the time of extraction is not particularly limited. For example, extraction at room temperature (for example, extraction at 0 to 40 ° C.) or heat extraction at 30 to 70 ° C. is preferable.

The extract of the lemon balm plant with the solvent may be in any form such as liquid, paste, gel, solid, and powder. The extract can be used as it is after preparation, but may be used after reconcentrating, drying or spray-drying it in water or a polar solvent.
Further, if necessary, a treatment such as decolorization, deodorization, or desalination may be performed using filtration, activated carbon, ion exchange resin or the like within a range that does not affect the effects of the present invention. It can also be used after fractionation by a method such as ultrafiltration, ion exchange chromatography, or gel filtration chromatography.

  The content (dry solid content) of the component (B) lemon balm plant or extract thereof of the present technology in the composition of the present technology is preferably 0.00001 to 1.0%, more preferably 0.0001 to 0.1%. And particularly preferably 0.001 to 0.01%.

  The content ratio (dry solid content) of the component (A) astaxanthin to the component (B) lemon balm plant or extract thereof in the composition of the present technology is not particularly limited, either. The content ratio by mass of A) / (B) is preferably 0.001 to 100, more preferably 0.01 to 10, and particularly preferably 0.02 to 3.

Furthermore, when collagen is contained as the component (C) in the present invention, the photostability of astaxanthin can be further enhanced. The collagen may be derived from marine organisms such as fish and jellyfish, may be derived from terrestrial organisms such as cows, pigs, and horses, or may be other. In addition, collagen may be used as it is, or may be used after being subjected to treatment such as atherolation, if necessary, and may be further subjected to hydrolysis by acid, alkali, enzyme treatment, or the like. it can. Among them, from the viewpoint of further increasing the stability, preferably, collagen, a hydrolyzate of collagen, and acylated atelocollagen; particularly preferably, water-soluble collagen, hydrolyzed collagen, atelocollagen, and succinyl atelocollagen are exemplified.

Examples of the collagen to be added to the composition of the present invention or the stabilizer for astaxanthin include Maringen S-03 (PF) (manufactured by Nitta Gelatin Co., Ltd.) and Maringen Peptide MP (PF) (manufactured by Nitta Gelatin Co., Ltd.) And commercial products such as shark fin telocollagen 1% PE (manufactured by Koken Co., Ltd.), porcine atelocollagen 1% PE (manufactured by Koken Co., Ltd.), Ocean Collagen (manufactured by Air Water Co., Ltd.), and Succinyl Ocean Collagen (manufactured by Air Water Co., Ltd.) It may be used, or one extracted according to a conventional method may be used.

The content of the component (C) may be appropriately set as necessary, and is, for example, 0.0000001 to 3% by weight, preferably 0.000001 to 1% by weight, and more preferably 0.00001 to 0.1%. % By weight, more preferably 0.00003 to 0.015% by weight, and particularly preferably 0.00015 to 0.015% by weight.

  The components of the present technology may contain, if necessary, components usually used for preparations of cosmetics, quasi-drugs, pharmaceuticals, etc. as long as the effects of the present invention are not impaired. As the component, for example, water (purified water, hot spring water, deep water, etc.), oil agent, surfactant, metal soap, gelling agent, powder, alcohols, water-soluble polymer, film forming agent, resin, ultraviolet light Protective agent, clathrate compound, antibacterial agent, fragrance, deodorant, salt, pH adjuster, freshener, animal / microbial extract, plant extract, blood circulation promoter, astringent, antiseborrheic, whitening agent , Anti-inflammatory agents, active oxygen scavengers, cell activators, humectants, chelating agents, keratolytic agents, enzymes, hormones, vitamins and the like.

The composition of the present technology can be obtained by a known production method. The composition of the present technology can be used in cosmetics, pharmaceuticals, foods and drinks, functional foods, skin external preparations, quasi-drugs, feeds, and the like. The compositions of the present technology can be used to make them.
Examples of the form in which the composition of the present technology is blended include cosmetics, quasi-drugs, pharmaceuticals, foods and drinks, functional foods (eg, supplements, foods for specified health use, etc.), animal feeds, and the like. Can be When blending the composition of the present technology into these forms, the composition may contain an appropriately acceptable component.
The present technology can be suitably used particularly for cosmetics and quasi-drugs. In this case, the component (A) and the component (B) are brought into contact with or applied to the skin. Specific examples of cosmetics and quasi-drugs include, for example, basic cosmetics such as milky lotions, creams, lotions, essences, packs, cleansers, makeup cosmetics such as foundations, blushes, lipsticks, and hair nourishes , Hair tonics, shampoos, rinses, and other hair cosmetics, dispersions, ointments, solutions, tablets, aerosols, patches, cataplasms, liniments and the like.

Conventionally, astaxanthins have useful effects as described above, but are difficult to obtain the expected effects due to their structural instability, and it is difficult to maintain the effects over a long period of time.
However, as shown in Examples below, the astaxanthins in the composition can be stabilized by blending the lemon balm plant or its extract with the composition containing the astaxanthins.

By using the component (B) of the present technology, the above-mentioned lemon balm plant or an extract thereof, it is possible to improve the stability of astaxanthins, and thereby it is possible to effectively exert the efficacy of astaxanthins. For example, the above-mentioned lemon balm plant or its extract can exert a remarkable effect on prevention of fading of astaxanthins and suppression of photodecomposition.
And astaxanthins are useful for prevention and improvement of rough skin and wrinkles, prevention and improvement of spots, and are also useful for prevention of aging and whitening, so the lemon balm plant or extract thereof has these effects of astaxanthins. (For example, an anti-aging effect and a whitening effect) can be dramatically improved. By using the lemon balm plant or the extract thereof, the effect of astaxanthins can be maintained for a longer period of time, or the same or more effect can be expected even if the content of astaxanthins is reduced.

Therefore, the composition containing the component (A) astaxanthins of the present technology and the component (B) Lemon balm plant of the family Labiatae or the extract thereof can stably and effectively stabilize the above-mentioned effects of the astaxanthins. Can be demonstrated.
In addition, since the lemon balm plant or the extract thereof of the present technology has a stabilizing effect on astaxanthins, the lemon balm plant or the extract thereof can be used as an astaxanthin stabilizer containing the lemon balm plant or the extract as an active ingredient. It is.
The lemon balm plant or extract thereof of the present technology may be used for stabilization of astaxanthins, and can be used for various preparations for stabilizing astaxanthins. It can also be used for manufacturing.
Therefore, the lemon balm plant of the present technology or an extract thereof is used for stabilizing astaxanthins, for external preparations for skin, cosmetics, pharmaceuticals, quasi-drugs, foods and drinks and functional foods (for example, foods for specified health use, supplements, etc.). Etc.).
The amount of use (dry solids) of the lemon balm plant or the extract thereof of the present technology is not particularly limited, but is preferably 0.01 to 1000 parts by mass, more preferably 0.1 to 1 part by mass of astaxanthins. １００100 parts by mass.

  Hereinafter, the present invention will be described more specifically with reference to Examples and the like, but the scope of the present invention is not limited thereto.

[Production Example 1: Production of lemon balm hot water extract 1]
Lemon balm (scientific name: Melissa officinalis) leaves (100 g) were cut into small pieces, added with 3000 mL of water, extracted at 90 ° C. for 6 hours, and filtered to remove insolubles, thereby obtaining a lemon balm extract 1. The dry solids content of this extract was 2.0%.

[Production Example 2: Production of lemon balm 50% ethanol extract 2]
100 g of lemon balm (scientific name: Melissa officinalis) is finely cut, 3000 mL of a 50% by volume aqueous solution of ethanol is added, and the mixture is extracted in a cool and dark place (4 to 10 ° C.) for 2 days. 2 was obtained. The dry solids content of this extract was 2.0%.

[Production Example 3: Production of 90% ethanol extract of lemon balm 3]
Lemon balm (Melissa officinalis) leaves (100 g) are finely cut, 3000 mL of a 90% by volume aqueous ethanol solution is added, extracted at about 55 ° C. for 24 hours, then left at 5 ° C. for 1 week, and insoluble substances are removed by filtration. Thus, a lemon balm 90% ethanol extract 3 was obtained. The dry solids content of this extract was 1.5%.

Example 1
<Test 1 for inhibiting photodecomposition (stabilizing effect) of astaxanthin>
0.00025% by mass of astaxanthin (manufactured by Wako Pure Chemical Industries), 0.00475% by mass of tri (capric acid / caprylic acid) glyceryl (manufactured by Nisshin Oillio), 25% by mass of dimethyl sulfoxide, lemon balm of Production Example 3 An aqueous solution containing the extract at a concentration of 0.00008 to 0.01% by mass as a dry solid content was prepared. 200 μL of each of these aqueous solutions was added to each 96-well plate, and the absorbance was measured. Thereafter, each solution was irradiated with ultraviolet light (UVA + B) three times for 5 minutes, and after the irradiation, the absorbance was measured. In addition, a solution to which no lemon balm plant extract was added was used as a control (0%). In addition, the content of astaxanthins in [Example] is astaxanthin-free conversion.

Astaxanthin residual rate is measured absorbance at 470nm of each sample,
Astaxanthin residual rate (%) = ((A−B) / (C−D)) × 100 (Formula 1)
A = absorbance of astaxanthin-containing system irradiated with ultraviolet light B = absorbance of astaxanthin-free system irradiated with ultraviolet light C = absorbance of astaxanthin-containing system not irradiated with ultraviolet light D = absorbance of astaxanthin-free system not irradiated with ultraviolet light

  The results are shown in Table 1. From these results, the lemon balm plant extract was able to suitably prevent the discoloration of astaxanthins under ultraviolet irradiation conditions. Astaxanthins are decomposed and discolored by ultraviolet rays, and their effects are reduced. This ultraviolet light has a more remarkable chemical action than sunlight. Therefore, the lemon balm plant extract had an excellent effect on suppressing the photolysis of astaxanthins. Therefore, the lemon balm plant extract can enhance the stability of astaxanthins and maintain its effect for a long period of time.

Example 2
<Test 2 for inhibiting photodecomposition (stabilizing effect) of astaxanthin>
0.00025% by mass of astaxanthin (manufactured by Wako Pure Chemical Industries), 0.00475% by mass of tri (capric acid / caprylic acid) glyceryl (manufactured by Nisshin Oillio), 25% by mass of dimethyl sulfoxide, lemon balm of Production Example 3 An aqueous solution containing 0.01% by mass of the extract as a dry solid content and 0.02% by mass of shark fin telocollagen (manufactured by Koken Co., Ltd.) or both was prepared. 200 μL of each of these aqueous solutions was added to each 96-well plate, and the absorbance was measured. Thereafter, each solution was irradiated with ultraviolet light (UVA + B) three times for 5 minutes, and after the irradiation, the absorbance was measured. In addition, a solution to which no lemon balm plant extract was added was used as a control (0%). In addition, the content of astaxanthins in [Example] is astaxanthin-free conversion.

Astaxanthin residual rate is measured absorbance at 470nm of each sample,
Astaxanthin residual rate (%) = ((A−B) / (C−D)) × 100 (Formula 1)
A = absorbance of astaxanthin-containing system irradiated with ultraviolet light B = absorbance of astaxanthin-free system irradiated with ultraviolet light C = absorbance of astaxanthin-containing system not irradiated with ultraviolet light D = absorbance of astaxanthin-free system not irradiated with ultraviolet light

The results are shown in Table 2. From these results, it was shown that the lemon balm plant extract suitably prevented the decomposition and fading of astaxanthin by ultraviolet rays. Further, from the results in Table 2, it was found that the discoloration inhibitory effect of astaxanthin was further enhanced by further combining collagen with the lemon balm plant extract. Therefore, by combining the lemon balm plant extract, or the lemon balm plant extract with collagen, an excellent effect on suppressing the photodecomposition of astaxanthins is exhibited, the stability of the astaxanthins is increased, and the effect can be maintained for a long time. It is possible.
As a comparative example, when 0.01 mM of ascorbic acid which is a commercially available antioxidant was tested in the same manner, the residual ratio of astaxanthin was about 44%. Therefore, 0.01% of the lemon balm plant extract contained 0% ascorbic acid. It can be said that the compound has an astaxanthin stabilizing effect superior to 0.01 mM.

Example 3 [Formulation in lotion]
(Ingredient) (%)
1 Glycerin 5.0
2 1,3-butylene glycol 5.0
3 Lactic acid 0.05
4 Sodium lactate 0.1
5 Water-soluble collagen (Note 1) 0.01
6 Lemon Balm Extract 1 0.05
7 Polyoxyethylene monooleate (20EO) sorbitan 1.2
8 Ethanol 8.0
9 Oil containing 5% astaxanthin (Note 2) 0.001
10 Methyl p-hydroxybenzoate 0.1
11 Fragrance 0.05
12 Remaining purified water
(Note 1) PANCOGEN MARINE (GATTEFOSSE)
(Note 2) Astaxanthin-5C (manufactured by Oriza Yuka)

(Production method)
A: Components (7) to (11) are mixed and dissolved.
B: Components (1) to (6) and (12) are mixed and dissolved.
C: A was added to B and mixed to obtain a lotion.

Example 4 [Formulation in emulsion]
(Ingredient) (%)
1 Polyoxyethylene monostearate (20EO) sorbitan 1.0
2 Polyoxyethylene trioleate (20EO) sorbitan 0.5
3 Glyceryl monostearate 1.0
4 Stearic acid 0.5
5 Behenyl alcohol 0.5
6 Squalane 8.0
7 Carboxyvinyl polymer 0.1
8 Methyl paraoxybenzoate 0.1
9 Sodium hydroxide 0.05
10 Purified water Remaining 11 Ethanol 5.0
12 lemon balm extract 2 0.01
13 Oil containing 1% astaxanthin (Note 3) 0.01
14 Fragrance 0.05
(Note 3) Astaxanthin derived from Adonis Palestinian flower

(Production method)
A: Components (1) to (6) are uniformly mixed at 70 ° C.
B: Mix components (7) to (10) uniformly at 70 ° C. C: Add A to B to emulsify and cool to room temperature.
D: (11) to (14) were added to C and mixed uniformly to obtain an emulsion.

Example 5 [Incorporation in Liquid Foundation (Oil-in-Water Cream)]
(Ingredient) (%)
11,3-butylene glycol 5.0
2 Hydrogenated soybean phospholipid 0.5
3 Titanium oxide 5.0
4 Bengala 0.1
5 Yellow iron oxide 1.0
6 Black iron oxide 0.05
7 Acrylic acid / alkyl methacrylate copolymer (Note 4) 0.5
8 Triethanolamine 1.5
9 Purified water Remaining 10 Glycerin 5.0
11 Ethyl paraoxybenzoate 0.1
12 Stearic acid 0.9
13 Glycerin monostearate 0.3
14 Cetostearyl alcohol 0.4
15 Polyoxyethylene monooleate (20EO) sorbitan 0.2
16 Polyoxyethylene trioleate (20EO) sorbitan 0.2
17 2-Ethylhexyl paramethoxycinnamate 5.0
18 Lemon Balm Extract 3 0.05
19 Oil containing 5% astaxanthin (Note 5) 0.05
20 fragrance 0.02
(Note 4) Pemulen TR-2 (NOVEON)
(Note 5) Astaxanthin derived from Haematococcus pluvialis

(Production method)
A: Components (1) to (6) are dispersed.
B: Add components (7) to (11) to A and mix uniformly at 70 ° C.
C: Components (12) to (17) are uniformly mixed at 70 ° C.
D: Add B to C, emulsify, and cool to room temperature.
E: Components (18) to (20) were added to D to obtain an oil-in-water creamy liquid foundation.

Example 6 [Formulation in sunscreen cosmetics (water-in-oil type cream)]
(Ingredient) (%)
1 Polyoxyethylene monolaurate (20EO) sorbitan 0.2
2 Polyoxyethylene (60) hydrogenated castor oil 0.1
3 Remaining purified water 4 Dipropylene glycol 10.0
5 Magnesium sulfate 0.5
6 Ascorbic acid glucoside 2.0
7 Lemon balm extract 1 0.3
8 Hydrolyzed collagen (Note 6) 0.02
9 PEG-9 polydimethylsiloxyethyl dimethicone 3.0
10 Decamethylcyclopentasiloxane 20.0
11 Isotridecyl isononanoate 5.0
12 2-Ethylhexyl paramethoxycinnamate 8.0
13 Oil containing 5% astaxanthin (Note 7) 0.01
14 Dimethyl stearyl ammonium hectorite 1.2
(Note 6) Marine Gen Peptide MP (PF) (manufactured by Nitta Gelatin Co., Ltd.)
(Note 7) ASTAX-S (Marine Daiosha)

(Production method)
A: Components (1) to (8) are uniformly dispersed.
B: Components (9) to (14) are uniformly dispersed.
C: While stirring B, A was gradually added and emulsified to obtain a water-in-oil cream sunscreen cosmetic.

Example 7 [Blending to ointment]
(Ingredient) (%)
1 Triethanolamine 2.0
2 Glycerin 5.0
3 Dipotassium glycyrrhizinate 0.5
4 Remaining purified water 5 Stearic acid 18.0
6 Cetanol 4.0
7 Lemon balm extract 2 0.05
8 Oil containing 20% astaxanthin (Note 8) 0.01
9 dl-α-tocopherol acetate 0.2
10 Methyl p-hydroxybenzoate 0.1
(Note 8) Astaxanthin-20C (manufactured by Oriza Yuka)

(Production method)
A: Components (1) to (4) are uniformly dissolved and kept at 75 ° C.
B: Components (5) to (10) are mixed by heating and kept at 75 ° C.
C: B was gradually added to A to obtain an ointment.

Example 8 [Blending to lotion]
(Ingredient) (%)
1 polyoxyethylene (20EO) sorbitan monolaurate
1.2
2 Ethanol 8.0
3 Oil containing 5% astaxanthin (Note 9) 0.001
4 Methyl paraoxybenzoate 0.2
5 Glycerin 5.0
6 1,3-butylene glycol 6.5
7 Lemon balm extract 1 0.03
8 Succinyl atelocollagen (Note 10) 0.05
9 Remaining purified water (Note 9) Astaxanthin derived from Fukuju (Note 10) Succinyl Ocean Collagen (Air Water Co., Ltd.)

(Production method)
A: Components (1) to (4) are mixed and dissolved.
B: Components (5) to (9) are mixed and dissolved.
C: A and B were mixed to obtain a uniform lotion.

Example 9 [Blending to Lip Cream]
(Ingredient) (%)
1 Candelilla wax 4.0
2 Ethylene / propylene copolymer 10.0
3 Pentaerythritol rosinate 5.0
4 Cetyl 2-ethylhexanoate 22.0
5 Vaseline 10.0
6 Liquid lanolin acetic acid 15.0
7 Diisostearyl malate 25.0
8 Diglyceryl triisostearate Remaining 9 Vitamin E 0.5
10 1,3-butylene glycol 1.0
11 Smoke-like anhydrous silicic acid 1.0
12 lemon balm extract 3 0.05
13 Oil containing 5% astaxanthin (Note 11) 0.3
13 Phenoxyethanol 0.3
14 dibutylhydroxytoluene 0.1
(Note 11) Krill-derived astaxanthin

(Production method)
A: Components (1) to (9) are uniformly dissolved and mixed at 100 ° C.
B: Add (10), (11) and (12) and mix uniformly.
C: B, (13) and (14) are added to A and mixed and dispersed.
D: After defoaming, C was filled in a container and cooled to obtain a lip balm.

The lotions, emulsions, oil-in-water creamy liquid foundations, water-in-oil creamy sunscreen cosmetics, ointments, lotions, and lip balms described in Examples 3 to 9 all have the effect of inhibiting the photodegradation of astaxanthin. It was excellent and maintained stability, and was useful for exerting effects on the skin such as improvement of rough skin, prevention of aging, and prevention and improvement of spots.

A composition characterized by containing the astaxanthins of the present technology and the Labiatae L. spp.lemon balm plant or an extract thereof, and a stabilizer and a stabilization method for the astaxanthins using the lemon balm plant or the extract thereof It can improve the efficacy of astaxanthins, and is useful for, for example, exerting effects on the skin such as prevention of aging and prevention and improvement of spots. Since the lemon balm plant is also used as a food, it is highly safe, so cosmetics containing the lemon balm plant or an extract thereof, an external preparation for the skin, pharmaceuticals, food and drink, and feed are expected to have high safety. be able to.

Claims (7)

Sheet containing source family perfume genus Mentha lemon balm plant or an extract thereof, inhibitors of photodegradation of astaxanthin. Agent according to claim 1, further comprising a collagen in further. Collagen is soluble collagen, hydrolyzed collagen, atelocollagen, agent according to claim 2, wherein at least one member selected from succinyl atelocollagen. Decomposition for suppressing the by luer Sutakisanchi in to ultraviolet light, agent according to any one of claims 1 to 3. The agent according to any one of claims 1 to 4, for a cosmetic, a skin external preparation or a quasi-drug containing astaxanthin . Labiatae Perfume genus Mentha lemon balm plant or method of inhibiting photodecomposition of astaxanthin using the extract. For suppressing decomposition by ultraviolet light of astaxanthin, methods who claim 6.