JP6666080B2 - Wrinkle improving method, wrinkle improving function improving method, wrinkle improving cosmetic, wrinkle improving function improving cosmetic, use thereof, and method for producing wrinkle improving cosmetic - Google Patents

Description

  The present invention relates to a wrinkle improving method. More specifically, a wrinkle improving method that exhibits a superior wrinkle improving effect compared to conventional techniques, a wrinkle improving function improving method, a wrinkle improving cosmetic, a wrinkle improving function improving cosmetic, and use thereof, and for wrinkle improving The present invention relates to a method for producing a cosmetic.

Maintaining healthy and beautiful skin is a problem of great interest, especially for women. However, the condition of the skin is constantly affected by factors such as humidity, ultraviolet rays, cosmetics, aging, illness, stress, and eating habits, and as a result, various skin troubles such as decreased skin functions and aging of the skin. Occurs.
Among these troubles, wrinkles are known to be caused by aging of the skin due to aging, photoaging due to exposure to sunlight, and the like.

  Conventionally, various methods for improving wrinkles have been studied. For example, a method is known in which dermis tissue is proliferated using a retinoid or retinoic acid to improve wrinkles (for example, Patent Documents 1 and 2). Also, a method of improving wrinkles by blending collagen or hyaluronic acid is known (for example, Patent Documents 3 and 4).

  In addition, in recent years, cosmetics exhibiting a wrinkle improving effect using an antioxidant have been developed. For example, Patent Literature 5 discloses a cosmetic containing fructosyl dipeptide and an antioxidant, and Patent Literature 6 discloses a cosmetic containing astaxanthin and tocotrienol.

  As a method other than the method using a cosmetic, for example, Patent Literature 7 discloses a method of performing light rejuvenation, wrinkle removal, and the like by irradiating light having a maximum intensity at about 630 nm.

JP-A-62-185005 JP-A-2-288822 JP-B-33-500 Japanese Patent Publication No. 4-65047 JP 2007-8847 A JP 2003-55188 A JP 2006-519047 A

  As described above, wrinkle improvement methods have been conventionally developed from various directions, but the wrinkle improvement effect is not sufficient with the conventional methods, and a more effective wrinkle improvement method has been desired.

  Therefore, it is a main object of the present invention to provide a wrinkle improvement technique that exhibits a superior wrinkle improvement effect as compared with the conventional technique.

  The present inventors have conducted intensive studies on a method for exhibiting wrinkle improvement effects, cosmetics to be used, and the like. As a result, irradiation of light having at least one emission peak at a wavelength of 405 to 904 nm and skin application of tocotrienol were each performed. The inventors have found that the effect of improving wrinkles is synergistically improved more than expected, and have completed the present invention.

That is, in the present invention, first, a light irradiation step of irradiating the skin with light having at least one emission peak at a wavelength of 405 to 904 nm,
An application step of applying tocotrienol to the skin,
Is provided at least.
Also, a method for improving the wrinkle improving function of tocotrienol,
A light irradiation step of irradiating the skin with light having at least one emission peak at a wavelength of 405 to 904 nm,
An application step of applying tocotrienol to the skin,
And a method for improving the wrinkle improving function of tocotrienol.

The present invention also provides a tocotrienol-containing wrinkle improving cosmetic which is applied before and / or after irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm.
Further, a cosmetic that improves a wrinkle improving function by irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm,
A wrinkle improving function improving cosmetic containing tocotrienol is also provided.

Further, a method of applying the wrinkle improving cosmetic or the wrinkle improving function improving cosmetic to the skin,
An application method for applying the wrinkle improving cosmetic or the wrinkle improving function improving cosmetic to the skin for at least two weeks or more continuously is also provided.
In the application method according to the present invention, the effect of the present invention can be obtained by applying the wrinkle improving cosmetic or the wrinkle improving function improving cosmetic to the skin continuously for at least two weeks or more. For example, before and / or after irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm for wrinkle improvement, the wrinkle improving cosmetic or the wrinkle improving function improving cosmetic is continuously used for at least 2 weeks or more. And applied to the skin.

Furthermore, the present invention also provides use of tocotrienol before and / or after irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm for wrinkle improvement.
The present invention also provides use of the wrinkle improving cosmetic before and / or after irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm for wrinkle improvement.

In addition, the present invention provides a method for producing a wrinkle improving cosmetic, which is applied before and / or after irradiation with light having at least one emission peak at a wavelength of 405 to 904 nm,
Perform at least an addition step of adding tocotrienol,
Also provided is a method for producing a cosmetic for improving wrinkles.

  In the present invention described above, as light having at least one emission peak at a wavelength of 405 to 904 nm, for example, red light having at least one emission peak at a wavelength of 590 to 750 nm can be used. The red light can be emitted using, for example, a red LED.

  ADVANTAGE OF THE INVENTION According to this invention, compared with the conventional wrinkle improvement technique, it is possible to obtain a much higher wrinkle improvement effect.

FIG. 4 is a schematic diagram schematically illustrating a test method in Experimental Example 1. 6 is a drawing-substituting graph showing a measurement result of a total wrinkle average depth (μm) in Experimental Example 1. 6 is a drawing-substituting graph showing a measurement result of a maximum wrinkle average depth (μm) in Experimental Example 1. 6 is a drawing-substitute graph showing measurement results of wrinkle volume (mm ³ ) in Experimental Example 1. 6 is a drawing-substitute graph showing measurement results of surface roughness (μm) in Experimental Example 1. 9 is a drawing substitute graph showing a questionnaire result of a feeling of effect in Experimental Example 1. FIG. 10 is a drawing-substituting graph showing the amount of change (μm) in the average wrinkle depth in Experimental Example 2. FIG. 9 is a graph substituted for a drawing showing the amount of change (μm) in the maximum wrinkle average depth in Experimental Example 2. 9 is a graph as a substitute of a drawing, showing a change amount (mm ³ ) of a wrinkle volume in Experimental Example 2. 9 is a graph as a substitute of a drawing, showing a variation (μm) in surface roughness in Experimental Example 2.

  Hereinafter, a preferred embodiment for carrying out the present invention will be described. The embodiment described below is an example of a typical embodiment of the present invention, and the scope of the present invention is not construed as being narrow.

1. Wrinkle improvement method, wrinkle improvement function improvement method The wrinkle improvement method according to the present invention irradiates the skin with light having at least one emission peak at a wavelength of 405 to 904 nm (hereinafter, also referred to as “light of a wavelength of 405 to 904 nm”). At least a light irradiation step and an application step of applying tocotrienol to the skin are performed. In the wrinkle improvement method according to the present invention, by performing the above two steps in combination, an excellent wrinkle improvement effect can be obtained due to a synergistic effect of both steps. In the present invention, either step may be performed first.

  The method for improving wrinkle-improving function according to the present invention is a method for improving the wrinkle-improving function of tocotrienol, which comprises irradiating the skin with light having a wavelength of 405 to 904 nm and applying the tocotrienol to the skin. Is performed at least. The method for improving wrinkle improvement function according to the present invention is to perform the two steps described above in combination, thereby obtaining an excellent wrinkle improvement effect by a synergistic effect of both steps, and improving the wrinkle improvement function of tocotrienol. Is possible. In the present invention, either step may be performed first.

  Hereinafter, each step of the wrinkle improvement method and the wrinkle improvement function improvement method according to the present invention will be described in detail.

(1) Light Irradiation Step In the present invention, the light irradiation step is a step of irradiating the skin with light having a wavelength of 405 to 904 nm.

  The type of light used in the light irradiation step of the present invention is not limited as long as the light has a wavelength of 405 to 904 nm, and the color and visibility of the light are not limited. Light emitted from a known light source can be freely selected and used. Can be. For example, visible light, invisible light, laser light, LED light, and the like can be given. In the present invention, it is particularly preferable to use red light having at least one emission peak at a wavelength of 590 to 750 nm (hereinafter, also referred to as “red light having a wavelength of 590 to 750 nm”). In this case, the irradiation of the red light can be performed by freely using a known light source capable of emitting the red light of the wavelength. In the present invention, the irradiation of the red light is particularly performed using a red LED. Is preferably performed. By using red light having a wavelength of 590 to 750 nm, a higher wrinkle improvement function can be exhibited. In addition, unlike a light source such as a fluorescent lamp or an incandescent lamp, an LED can easily obtain light containing only light of a desired wavelength without light of an unnecessary wavelength. Can be irradiated.

  At present, LEDs (light emitting diodes) of various colors such as blue, green, red, yellow, and white are known due to the difference in wavelength and the mixing method. In the present invention, red LEDs are used. Is more preferable. When the wavelength of light emitted from the compound constituting the LED is around 660 nm, the compound emits red light. The red LED is generally used for illumination, an indicator, a tail lamp of an automobile, a light source of a portion for displaying red such as a traffic light, and the like.

  The specific wavelength of the red LED that can be used in the present invention is not particularly limited as long as it is in a range in which red light is emitted, and can be freely set according to the purpose and the specifications of the device to be used. For example, a red LED having a wavelength of 600 to 700 nm can be used.

In the wrinkle improvement method and the wrinkle improvement function improvement method according to the present invention, the mode of irradiating light having a wavelength of 405 to 904 nm is not particularly limited, and a device or the like including light having a wavelength of 405 to 904 nm may be directly contacted with the skin. Irradiation may be performed in any suitable manner, or the device or the like may be installed at a location away from the skin and irradiated. Further, the irradiation time of the LED light to the skin, the intensity of the irradiated light, and the like are not particularly limited. For example, as irradiation conditions for one irradiation, irradiation time: 1 to 80 minutes, preferably 5 to 60 minutes, illuminance: 0.01 to 200 mW / cm ² , preferably 1 to 110 mW / cm ² , irradiation intensity: 0. It can be set to 01 to 200 J / cm ² , preferably 1 to 140 J / cm ² . Further, the number of times of LED light irradiation is not particularly limited, but in the present invention, it is particularly preferably 1 to 10 times, more preferably 2 to 8 times, and still more preferably 3 to 6 times. In addition, the irradiation frequency of the LED light is not particularly limited, but in the present invention, it is particularly preferable to irradiate every 1 to 3 weeks.

(2) Application Step In the present invention, the application step is a step of applying tocotrienol to the skin.
In the wrinkle improving method and the wrinkle improving function improving method according to the present invention, the mode of applying tocotrienol to the skin is not particularly limited. For example, the tocotrienol can be contained in cosmetics and applied to the skin.

  Tocotrienol is a generic term for isomers of tocopherol (vitamin E) and is a naturally occurring component contained in wheat, rice bran, palm oil and the like. Further, tocotrienol includes α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol and the like. In the present invention, these can be used alone or as a mixture of two or more. The tocotrienol used in the present invention is not particularly limited, but is preferably a mixture of the above four types of tocotrienol from the viewpoints of easy availability of raw materials, high purity at the time of purification, safety to living bodies, and the like.

  In the present invention, the period of application of tocotrienol to the skin is not particularly limited, and can be freely set according to the intended effect, the number of irradiations of light, the frequency of irradiation, and the like. In the present invention, in particular, it is preferable to apply continuously for one week or more, and it is more preferable to apply continuously for two weeks or more. In this case, "continuously applying" means applying at least so that tocotrienol stays on and / or in the skin, and is applied once to several times a day or one to several days. It also includes a method of applying it to other places.

2. Wrinkle improving cosmetic, wrinkle improving function improving cosmetic The wrinkle improving cosmetic according to the present invention is applied before and / or after irradiation with light having a wavelength of 405 to 904 nm, and contains tocotrienol. I do. By applying the wrinkle improving cosmetic composition according to the present invention before and / or after irradiation with light having a wavelength of 405 to 904 nm, an excellent wrinkle improving effect can be obtained.

  Further, the cosmetic for improving wrinkle improving function according to the present invention is a cosmetic for improving wrinkle improving function by light irradiation at a wavelength of 405 to 904 nm, and is characterized by containing tocotrienol. By applying the cosmetic for improving wrinkle improvement function according to the present invention before and / or after irradiation with light having a wavelength of 405 to 904 nm, it becomes possible to obtain an excellent wrinkle improvement effect, and a wavelength of 405 to 904 nm. It is possible to further improve the effect of wrinkle improvement by light irradiation.

  The wrinkle improving cosmetic or the wrinkle improving function improving cosmetic according to the present invention (hereinafter, also referred to as “cosmetic according to the present invention”) can be applied to any form of cosmetic. For example, skin care cosmetics such as lotion, milky lotion, cream, eye cream, serum, massage, pack, hand cream, body cream, sunscreen, facial cleanser, powder foundation, liquid foundation, makeup base It can be applied to makeup, white powder, concealer, makeup cosmetics such as eyeshadow, and the like.

  In the cosmetics according to the present invention, it is possible to freely select one or more components that can be used for cosmetics in addition to tocotrienol. For example, bases, preservatives, emulsifiers, coloring agents, preservatives, surfactants, ultraviolet absorbers, antioxidants, humectants, ultraviolet absorbers, fragrances, antiseptic / antifungal agents, extender pigments, coloring pigments, alcohols, All additives usually used in the cosmetics field, such as polyhydric alcohols, water, oils, thickeners, powders, chelating agents, enzymes and pH adjusters, can be contained.

  Moreover, since tocotrienol contained in the cosmetic according to the present invention is a naturally-derived component as described above, there is little need to be careful when used in combination with other active ingredients. Therefore, in addition to tocotrienol, other active ingredients can be freely added to the cosmetic according to the present invention as needed. For example, any active ingredient such as an antioxidant, an anti-inflammatory agent, a cell activator, an ultraviolet ray inhibitor, a humectant, a blood circulation promoter, an antibacterial agent, a whitening agent, and an active enzyme remover can be blended.

  Examples of antioxidants include vitamin C and its derivatives, carotenoids, dibutylhydroxytoluene, butylhydroxyanisole, rutin and its derivatives, glutathione and its derivatives, superoxide dismutase, mannitol, calyx extract, gennoshoko extract, hawthorn extract Extract, peonies extract, ginkgo extract, Scutellaria (Oregon) extract, Maikaika (Makai, Hamanas) extract, Tormentilla extract, grape extract, Yasajitsu (Yasa) extract, Yukinoshita extract, Rosemary (Mannenrou) Extract, tea extract (such as oolong tea, black tea, green tea, etc.).

  Examples of the anti-inflammatory agent include glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, sulfur and its derivatives, aloe extract, Ashitaba extract, nettle extract, cinnamon (Kawarayogi) extract, turmeric extract, yellowfin ( Ole) extract, Hypericum perforatum extract, chamomile extract, Comfrey (Hypericum perforatum) extract, honeysuckle (Kingenka) extract, watercress extract, Salvia (sage) extract, Waremokou (Jiyu) extract, perilla extract, Examples include birch extract, elder extract, cattail (squirt) extract, sapling extract, eucalyptus extract, mugwort extract, astragalus extract, Kumazasa extract, and zinc oxide.

  Examples of cell activators include caffeine, cockscomb extract, shell extract, shellfish extract, royal jelly, silk protein and its degradation products or their derivatives, lactoferrin or its degradation products, chondroitin sulfate, hyaluronic acid, etc. Mucopolysaccharides or salts thereof, collagen, yeast extract, lactic acid bacteria extract, bifidobacterium extract, fermentation metabolic extract, ginkgo extract, barley extract, assembly extract, diatom extract, carrot extract, glycolic acid , Citric acid, lactic acid, malic acid, tartaric acid, vitamin A succinate and its derivatives, asparagus extract, soybean extract, jujube extract, garlic extract, beech extract, microbial fermentation metabolite, reishi Extract and the like.

Examples of UV inhibitors include 2-ethylhexyl paramethoxycinnamate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sodium sulfate, 4-t-butyl-4'- Examples include methoxydibenzoylmethane, 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, zinc oxide, oxybenzone and derivatives thereof, fine titanium oxide and fine zinc oxide. The use of fine particles of inorganic powder such as titanium oxide and zinc oxide provides a higher effect.

  Examples of humectants include proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, glycine, serine, aspartic acid, glutamic acid, arginine, amino acids such as theanine and derivatives thereof, sorbitol, erythritol, Trehalose, inositol, glucose, sucrose and its derivatives, dextrin and its derivatives, saccharides such as honey, D-panthenol and its derivatives, urea, phospholipids, ceramide, spinach extract, shobu extract, germ extract, senkyu extract Products, mallow extract, lycopodium extract, dokudami extract, hamamelis extract, bodaige extract, marronnier extract, quince extract and the like.

  Examples of the blood circulation promoting agent include yuzu extract, arnica extract, pepper tincture, shobu extract, γ-oryzanol and the like.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, p-hydroxybenzoic acid ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol and the like.

  Examples of whitening agents include ascorbic acid or a derivative thereof, arbutin, ellagic acid, linoleic acid, glycyrrhizic acid and its derivatives, tranexamic acid, placental extract, chamomile extract, licorice extract, edible extract, orgonum extract, Seaweed extract, kudin extract, calyx extract, gokahi extract, rice bran extract, wheat germ extract, saishin extract, hawthorn extract, sempens extract, white lily extract, peony extract, Sempukuka extract, soybean Extracts, tea extracts, molasses extracts, juniper extracts, grape extracts, hop extracts, Maika mosquito extracts, mocca extracts, Saxifraga extracts, rosemary extracts and the like.

  As an example of the active oxygen removing agent, any agent having an action such as suppression of lipid peroxide production can be freely selected and used, for example, superoxide dismutase, mannitol, quercetin, catechin and the like. Derivatives, rutin and its derivatives, peanut extract, yasazi extract, melissa extract, arhat extract, retinol and its derivatives, vitamins A such as carotenoids, thiamine and its derivatives, riboflavin and its derivatives, pyridoxine and its derivatives, Vitamin Bs such as nicotinic acid and derivatives thereof, dibutylhydroxytoluene and butylhydroxyanisole.

  In the cosmetic according to the present invention, the amount of tocotrienol is not particularly limited and can be freely set as appropriate, but is preferably contained in an amount of 0.01% by mass or more and 10% by mass or less, % Or more and 1% by mass or less. Within this range, the effect of improving wrinkles can be sufficiently exhibited.

  Since the cosmetic according to the present invention is a cosmetic mainly containing tocotrienol, which is a naturally derived component, it has high safety and can be used continuously for a long period of time.

3. Application method The application method according to the present invention is a method for applying the wrinkle improving cosmetics according to the present invention or the wrinkle improving function improving cosmetics according to the present invention to the skin, and is applied continuously for at least two weeks or more. It is characterized by the following. By using the coating method according to the present invention, wrinkles can be more effectively improved when the cosmetic according to the present invention is used. "Applying for 2 weeks continuously" means that tocotrienol is applied at least on the skin and / or in the skin so that it stays for at least 2 weeks. A method of applying every few days or the like is also included.

  The application method according to the present invention requires the cosmetic according to the present invention to be applied continuously for at least two weeks, but it is also possible to apply the cosmetic according to the present invention continuously for a longer period. It is. As described above, the cosmetic according to the present invention is a cosmetic mainly containing tocotrienol, which is a naturally-derived component, and therefore has high safety and is excellent in long-term continuous use.

  In the application method according to the present invention, if the cosmetic according to the present invention is applied continuously for at least two weeks or more, the order of irradiation with light having a wavelength of 405 to 904 nm is not particularly limited. For example, a method of irradiating light of a wavelength of 405 to 904 nm a required number of times during continuous application of the cosmetic of the present invention for two weeks, wavelength of 405 to 904 nm after applying the cosmetic of the present invention for two weeks continuously , A method of continuously applying the cosmetic according to the present invention for two weeks after irradiating light having a wavelength of 405 to 904 nm, a method of combining these methods, and the like can be freely set. it can.

4. Use of Tocotrienol, Use of Cosmetic for Improving Wrinkles or Use of Cosmetic for Improving Wrinkles The use of the tocotrienol according to the present invention is performed before and / or after irradiation with light having a wavelength of 405 to 904 nm for improving wrinkles. It is characterized by being used. In addition, the use of the cosmetic according to the present invention is achieved by improving the wrinkle improving cosmetic or the wrinkle improving function according to the present invention before and / or after irradiation with light having a wavelength of 405 to 904 nm for wrinkle improvement. It is characterized by using cosmetics. By using these according to the present invention, it becomes possible to obtain an excellent wrinkle improvement effect by synergistic effect of irradiation of light having a wavelength of 405 to 904 nm and tocotrienol.

5. The method for producing a cosmetic for improving wrinkles is a method for producing a cosmetic for improving wrinkles, which is applied before and / or after irradiation with light having a wavelength of 405 to 904 nm, wherein at least tocotrienol is added. It is characterized in that an addition step is performed.

  In the production method according to the present invention, the point at which the addition step is performed is not particularly limited. In the addition step, only tocotrienol may be added, or one or two or more of the above-mentioned components or other active ingredients which can be used in ordinary cosmetics may be freely selected and appropriately selected. It is also possible to add.

  Hereinafter, the present invention will be described in more detail based on examples. The embodiment described below is an example of a typical embodiment of the present invention, and the scope of the present invention is not construed as being narrow.

1. Experimental Example 1: Examination of Wrinkle Improvement Effect (1) Test Method In this experiment, red light emitted from a red LED was used as an example of light having a wavelength of 405 to 904 nm. Using a device (device name: Omnilux Rev, wavelength: 633 nm) capable of irradiating red LED light, a light intensity of 126 J / cm ² for 20 minutes was measured every two weeks as shown in FIG. Five times, all the faces of the 18 subjects were irradiated with red LED light. The irradiation of the red LED light by the device was performed at a distance of about 3 to 5 cm from the skin of the subject. After the first irradiation, the tocotrienol preparation prepared by the following method was applied twice daily in the morning and evening only to the half face of the subject, and nothing was applied to the other half face. Furthermore, even after irradiation of red LED light five times, the tocotrienol preparation was continuously applied for two months.

In addition, the tocotrienol preparation used what was prepared by the following manufacturing method using the following prescription.
<Prescription>
(Component): (% by mass)
1. Hydrogenated soybean phospholipid: 0.8
2. Cholesterol: 0.2
3. Polyoxyethylene monooleate (20 mol) sorbitan: 1
4. Glycerin: 10
5. 1,3-butylene glycol: 10
6. Methyl paraoxybenzoate: 0.1
7. Liquid paraffin: 5
8. Hexa (hydroxystearic acid / stearic acid / rosinic acid) dipentaerythrityl: 3
9. Dimethyl polysiloxane (Note 1): 0.5
10. Cetostearyl alcohol: 2
11. Tocotrienol (Note 2): 0.3
12. Carbomer (Note 3): 0.1
13. (Acrylates / alkyl acrylate (C10-30)) crosspolymer (Note 4): 0.1
14． Sodium hydroxide: 0.06
15. Ethanol: 10
16. Purified water: remaining amount Note 1: KF-96A-100CS (manufactured by Shin-Etsu Chemical Co., Ltd.)
Note 2: Tricot 92 (Eisai, tocotrienol purity: 70%)
Note 3: CARBOPOL 980 (manufactured by LUBRIZOL ADVANCED MATERIALS)
Note 4: CARBOPOL1382 (manufactured by LUBRIZOL ADVANCED MATERIALS)
<Production method>
A: Components 1 to 5 were uniformly mixed at 80 ° C.
B: Components 6 to 11 were uniformly mixed at 80 ° C.
C: B was added to A and mixed uniformly.
D: Components 12 to 16 were added to C and mixed uniformly to obtain a tocotrienol preparation.

(2) Evaluation The wrinkle improvement effect was evaluated before the first irradiation and before the third irradiation as shown in FIG. 1 in order to verify the synergistic effect of the irradiation of the red LED light and the cosmetic for wrinkle improvement according to the present invention. The test was performed two weeks after the fifth and fifth irradiations, and one month and two months later, in order to compare the durability of the wrinkle improving effect.

Specifically, the subject was placed in a resting state with his eyes lightly closed in a sitting position, and a replica from the outer corner of the eye to below the eye was collected using a replica agent (SILFLO (manufactured by Flexico Developments, UK)). About the collected replica, the total wrinkle average depth (μm), the maximum wrinkle average depth (μm), the wrinkle volume (mm ³ ), and the surface roughness (μm) were measured by using a measuring instrument for analysis (PRIMOS (manufactured by GFMestechnik)). ) Were measured for all four items.

  In addition, subjects were given questionnaire surveys on the feeling of effectiveness and satisfaction before the third irradiation, two weeks after the fifth irradiation, and one month and two months after the fifth irradiation. The therapeutic effect on the pores and the persistence of the effect were investigated.

(3) Results The measurement results (average values of 18 subjects) obtained by the above test method are shown in FIGS. FIGS. 2 to 5 show only the red LED light irradiation for each item of total wrinkle average depth (μm), maximum wrinkle average depth (μm), wrinkle volume (mm ³ ), and surface roughness (μm). It is the graph which compared the measurement result of the site | part (indicated by "absence" in a graph) with the measurement result of the site | part which applied the tocotrienol formulation in addition to red LED light irradiation (indicated by "presence" in a graph). FIG. 6 shows the results of the feeling of effect obtained from the subject.

(4) Discussion Comparing the site where the tocotrienol preparation was applied and the site where the application was not applied, the site where the tocotrienol preparation was applied was compared with the site where the tocotrienol preparation was not applied, and in all items, the red LED light irradiation was performed. It was found that each value had a decreasing tendency from the start time. As a result of analysis using the t-test, a significant difference was found particularly in the items of maximum wrinkle average depth (μm), wrinkle volume (mm ³ ), and surface roughness (μm). In addition, significant differences were observed in the maximum wrinkle average depth (μm) and the wrinkle volume (mm ³ ) two weeks after the fifth irradiation, and in the surface roughness (μm) after one month. From the above results, it has been clarified that the application of tocotrienol synergistically enhances the wrinkle improvement effect as compared with the irradiation of only red LED light.

  As shown in FIG. 6, in the questionnaire on the effect of the subject, the effect feeling “slightly inconspicuous” can be obtained high only by the red LED light irradiation, but the effect feeling “slightly inconspicuous” Compared to irradiation alone, the use of the application of the tocotrienol preparation was clearly higher in evaluation. In addition, in the case of using the tocotrienol preparation in combination, there was a subject who had a feeling of "obviously inconspicuous", but no effect was obtained at all only with red LED light irradiation.

2. Experimental Example 2: Investigation of synergistic effect (1) Test method When only tocotrienol preparation was applied for 4 weeks (tocotrienol alone group), when only irradiation with red LED light was performed for 4 weeks (red LED alone group), In the case where the application and the irradiation of the red LED light were performed for 4 weeks (combination group), the difference in the effect was verified. Specifically, the application of the tocotrienol preparation and / or the irradiation of red LED light were performed on seven subjects in each group. The formulation of the tocotrienol preparation, the device for irradiating red LED light, and the irradiation conditions are the same as those in Experimental Example 1.

(2) Evaluation For each group, before the start of the test and after the end of the test (four weeks), replicas were collected in the same manner as in Experimental Example 1. For the collected replica, a total wrinkle average depth (μm), a maximum wrinkle average depth (μm), and a wrinkle volume (mm ³ ) were measured using a pattern projection type three-dimensional measuring device (trade name “PRIMOS PICO” manufactured by GFMestechnik). ) And surface roughness (μm) were measured.

(3) Results With respect to the measurement results (average of seven subjects) obtained by the above test method, the amount of change between before the start of the test and after the end of the test (four weeks) was calculated. The change amounts calculated for each item are shown in FIGS.

(4) Discussion As shown in FIGS. 7 to 10, the combined group in which the application of the tocotrienol preparation and the irradiation of the red LED light were performed showed, in all items, an effect that was greater than the additive effect of the tocotrienol alone group and the red LED alone group, That is, a synergistic effect could be confirmed. For example, as shown in FIG. 9, the wrinkle volume is about −0.25 even when the change amount of the tocotrienol alone group and the change amount of the red LED alone group are added. The combined group irradiated with red LED light had a change of -0.36.

From the above results, by using the application of the tocotrienol preparation and the irradiation of the red LED light in combination, the application of the tocotrienol preparation alone and the effect of improving wrinkles more than the effect expected from the single irradiation of the red LED light can be exhibited. Proven.

Claims (3)

A light irradiation step of irradiating the skin with only red LED light having a wavelength of 600 to 700 nm,
An application step of applying tocotrienol to the skin,
At least a wrinkle improvement method.   The wrinkle improvement method according to claim 1, wherein the light irradiation step is performed 3 to 6 times every 1 to 3 weeks.   The wrinkle improvement method according to claim 1, wherein the application step is performed continuously for two weeks or more.

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