JP6668080B2 - Humidifying bag and incubator with humidifying bag - Google Patents

Description

  The present invention relates to a device for culturing a culture such as cells, for example, a humidification bag that is disposed in a culture chamber of an incubator and humidifies a culture environment, and an incubator provided with the humidification bag.

  A device for culturing a culture, such as an incubator, is used for culturing a culture such as human cells or animal cells. As an example of an incubator, Patent Document 1 discloses a CO2 incubator that supplies carbon dioxide (CO2) gas into a culture chamber.

  In a culture room of a CO2 incubator, a humidity environment of 90% or more is required for culturing a culture. In order to obtain a humidity environment of 90% or more in the culture room, usually, a tray-type humidification vat without an upper lid is prepared, water is poured into the humidification vat, and the humidification vat containing the water is placed in the culture room of the CO2 incubator. Put in. Thus, the culture chamber is humidified by the generation of water vapor, and a humidity environment of 90% or more can be obtained.

JP 2010-154793 A

  By the way, when an operator carries a humidification bat with water in a tray-type humidification bat without an upper lid, it is difficult to handle because the water easily leaks from the humidification bat. In addition, in a state where the humidified bat containing water is placed in the culture chamber, there is a problem that water is spilled from the humidified bat in the culture chamber when an external force such as an earthquake is applied to the CO2 incubator.

  The present invention has been made in view of the above, and an object of the present invention is to eliminate the spillage of a liquid such as water inside a device such as a culture room, so that it is easy to handle, and the inside of the device can be easily formed. An object of the present invention is to provide a humidifying bag capable of humidifying and an incubator provided with the humidifying bag.

  In order to achieve the above object, the humidifying bag according to claim 1 is a humidifying bag for humidifying the inside of the device, the bag body having an opening, and enclosing a liquid; and A gas permeable member disposed in the bag body so as to close the opening of the back body, and humidifying the inside of the device through a gas of the liquid without passing the liquid in the bag body; By covering the permeable member, the gas is prevented from flowing out of the bag body from the gas permeable member, and is removed when the inside of the device is humidified to expose the gas permeable member. And a prevention sheet.

  In the humidifying bag according to the first aspect, the liquid does not leak from the opening because the gas-permeable member does not pass the liquid when the bag body is filled with the liquid. Moreover, the gas permeation prevention sheet can prevent the liquid gas from leaking from the gas permeable member.

  For this reason, the humidification bag is easy to handle by completely preventing the liquid such as water from spilling inside the apparatus, and the liquid gas leaks from the gas permeable member if the gas permeation prevention sheet is removed. Therefore, the inside of the device can be easily humidified by the liquid gas.

  The humidifying bag according to claim 2, further comprising a covering sheet that is adhered on the gas permeation prevention sheet and is peeled off when the humidification is performed to remove the gas permeation prevention sheet from the gas permeable member. It is characterized by doing.

  In the humidifying bag according to the second aspect, when the inside of the device is humidified, by peeling off the covering sheet, the gas-permeable preventing sheet together with the covering sheet can be easily and reliably removed from the gas-permeable member. it can.

  In the humidifying bag according to claim 3, the gas permeation prevention sheet has a cut portion, and the cut portion is a peeling portion that is removed from the gas permeable member when the cover sheet is peeled off; The remaining portion remaining on the main body side is separated.

  In the humidifying bag according to the third aspect, when the inside of the apparatus is humidified, the cover sheet is peeled off, so that only the peeled portion of the gas permeation prevention sheet together with the cover sheet is easily and reliably separated from the gas permeable member. Can be removed.

  In the humidifying bag according to the fourth aspect, the bag body has an injection portion for injecting the liquid.

  In the humidifying bag according to the fourth aspect, when the operator humidifies the inside of the device, the operator can later inject any liquid necessary for humidification into the humidifying bag.

  An incubator provided with the humidifying bag according to claim 5, wherein the incubator has an interior provided with a predetermined humidity environment, and has an opening, a bag body for enclosing a liquid, and the opening of the back body. A gas permeable member that is disposed on the bag body so as to close a portion thereof and humidifies the inside of the incubator through a gas of the liquid without passing the liquid in the bag body; and covering the gas permeable member. A gas permeation prevention sheet that prevents the gas from going out of the bag main body from the gas permeable member and removes the humidified inside of the incubator to expose the gas permeable member. Humidifying bag.

  In the incubator provided with the humidifying bag according to the fifth aspect, the liquid does not leak from the opening since the gas-permeable member does not pass through the liquid when the bag body is filled with the liquid. Moreover, the gas permeation prevention sheet can prevent the liquid gas from leaking from the gas permeable member.

  For this reason, the humidifying bag can be easily handled by completely preventing the liquid such as water from spilling in the culture chamber of the incubator inside the apparatus, and the gas permeable member can be removed by removing the gas permeation prevention sheet. Liquid gas leaks out of the incubator, so that the liquid gas can easily humidify the culture chamber of the incubator.

  According to the present invention, there is provided a humidifying bag and a humidifying bag capable of easily humidifying the inside of the device by completely eliminating spillage of liquid such as water inside the device such as a culture room. Can provide an incubator.

FIG. 1 is a perspective view showing an appearance of a first embodiment of a preferable humidifying bag of the present invention. FIG. 2 is a perspective view showing an example of the shape of a bag body, a gas permeable film, and an opening of the bag body of the humidifying bag shown in FIG. FIG. 2 is an exploded perspective view showing a bag body, a gas permeable film, a water vapor permeation prevention sheet, and a covering sheet of the humidifying bag shown in FIG. FIG. 4 is a schematic view showing an example of a cross-sectional structure of the humidifying bag shown in FIGS. 1 and 3. It is a schematic diagram which shows the state which packaged the bag for humidification shown in FIG. 4 with the outer bag. It is a schematic diagram which shows the state which peeled the covering sheet from the bag main body and removed the peeling part of the water vapor permeation prevention sheet, and exposed the gas permeable film of the bag main body. It is a longitudinal section showing the preferred embodiment of the CO2 incubator to which the above-mentioned humidification bag is applied. It is a perspective view which shows 2nd Embodiment of the bag for humidification of this invention. It is a front view showing a 3rd embodiment of the bag for humidification of the present invention.

  Hereinafter, embodiments for carrying out the present invention (hereinafter, referred to as embodiments) will be described with reference to the drawings.

<First embodiment>
FIG. 1 is a perspective view showing an external appearance of a first embodiment of a preferred humidifying bag of the present invention. FIG. 2 is a perspective view showing an example of the shape of the bag body 2, the gas permeable film 3 having gas permeability, and the opening 4 of the bag body 2 of the humidifying bag 1 shown in FIG.

  The humidifying bag 1 shown in FIG. 1 and FIG. 2 is a liquid sealing bag for humidifying the culture chamber by being disposed inside the processing apparatus, for example, in the culture chamber of a CO2 incubator or in the culture chamber of a multi-incubator. is there.

  The bag body 2 of the humidifying bag 1 contains a liquid such as sterilized water. In at least a part of the humidifying bag 1, a gas permeable film 3 made of a material having gas permeability is arranged. The gas permeable film 3 is arranged so as to cover the opening 3 of the bag body 2.

  In an incubator, it is used for culturing cultures such as human cells or animal cells. In the culture room of the incubator, a humidity environment of 90% or more is required for culturing the culture, so the humidification bag 1 is placed in the culture room. Thereby, the steam of the sterilized water in the humidifying bag 1 can humidify the inside of the culture chamber from inside the bag main body 2 of the humidifying bag 1 through the gas permeable film 3 made of a material having gas permeability. However, since the gas permeable film 3 does not allow liquid sterilized water to pass through, the liquid sterilized water itself does not leak from the humidification bag 1 through the gas permeable film 3.

  Next, a preferred structure example of the humidifying bag 1 will be further described.

  The humidifying bag 1 shown in FIGS. 1 and 2 has a bag body 2. The bag body 2 is, for example, a heat-resistant plastic bag-shaped member. The bag body 2 is made of, for example, a transparent material, a polyethylene film, a vinyl film, or the like that does not leak the liquid to be put into the bag body 2. The bag body 2 is not limited to a transparent film, but may be made of a translucent or opaque film, and is not particularly limited in material.

  The material for forming the bag body 2 is, for example, formed in a tubular shape in advance, so that one end 2A and the other end 2B of the bag body 2 each have an opening. One end 2A of the bag body 2 is sealed by heat sealing, so that the opening of the one end 2A is closed in advance.

  For example, the inside of the bag body 2 is filled with sterile water from the opening of the other end 2B of the bag body 2, and then the other end 2B of the bag body 2 is sealed by heat sealing. 2 is filled with sterilized water. Thereby, sterilized water as a liquid for generating steam for humidification is sealed inside the bag body 2 so as not to leak.

  As shown in FIGS. 1 and 2, the bag body 2 is, for example, a rectangular bag-shaped member. The bag body 2 has a first surface 2C and a second surface 2D opposite to the first surface 2C. Having. The first surface portion 2C of the bag body 2 is provided with, for example, a rectangular or square opening 4. The area of the opening 4 can be set arbitrarily according to the required amount of sterilized water vapor in the first surface 2C.

  As shown in FIG. 2, the gas permeable film 3 is an example of a gas permeable member. The four sides of the gas permeable film 3 are tightly fixed to the inner surface of the first surface 2C on the inner surface side of the first surface 2C of the bag body 2 by, for example, heat sealing. The gas permeable film 3 is formed in a rectangular shape or a square shape according to the shape of the opening 4, and the vertical size and the horizontal size of the gas permeable film 3 The size of the opening 4 is set to be larger than the size in the vertical direction and the size in the horizontal direction so that it can be covered.

  Thereby, the gas permeable film 3 covers the opening 4 from the inner side of the first surface 2C. Therefore, the sterilized water in the bag body 2 does not leak out of the bag body 2 from between the gas permeable film 3 and the inner surface of the first surface portion 2C of the bag body 2.

  The gas-permeable film 3 is made of a material having a gas-permeable property that allows passage of water vapor (gas) of sterilized water W (liquid) but does not allow passage of a liquid, for example, a high-density polyethylene nonwoven fabric. . The gas permeable film 3 can also be referred to as a perforated film having minute holes that allow gas to pass but not liquid.

  Next, refer to FIG. 3 and FIG.

  FIG. 3 is an exploded perspective view showing the bag main body 2, the gas permeable film 3, the water vapor permeation prevention sheet 5, and the covering sheet 6 of the humidifying bag 1 shown in FIG.

  As shown in FIG. 3, the humidifying bag 1 preferably has a water vapor transmission preventing sheet 5 and a covering sheet 6 in addition to the bag body 2 and the gas permeable film 3. The water vapor permeation prevention sheet 5 is an example of a gas permeation prevention sheet.

  The water vapor permeation prevention sheet 5 is formed in a rectangular or square shape according to the shape of the opening 4 of the bag body 2. The vertical dimension and the horizontal dimension of the water vapor transmission preventing sheet 5 are set to the vertical dimension and the horizontal dimension of the opening 4 so that the opening 4 and the gas permeable film 3 can be reliably covered. In comparison, it is set to be large. The water vapor permeation prevention sheet 5 is a thin film made of, for example, plastic that is impermeable to water vapor. The covering sheet 6 is, for example, a rectangular or square sheet having a dimension capable of completely covering the water vapor transmission preventing sheet 5 from above.

  An adhesive portion 6B is formed on the entire surface of the back surface 6A of the cover sheet 6, and the cover sheet 6 completely covers the water vapor permeation prevention sheet 5 so that the outer surface of the water vapor permeation prevention sheet 5 and the bag body 2 To the outer surface of the first surface portion 2C. Thereby, the covering sheet 6 can completely cover the water vapor transmission preventing sheet 5 and the gas permeable film 3.

  FIG. 4 is a schematic view showing an example of a cross-sectional structure of the humidifying bag 1 shown in FIGS. FIG. 5 is a schematic view showing a state in which the humidifying bag 1 shown in FIG.

  As shown in FIG. 4, the gas permeable film 3 is tightly fixed to the inner surface 2E side of the first surface 2C of the bag body 2 so as to correspond to the opening 4 of the bag body 2. On the other hand, the water vapor permeation prevention sheet 5 is tightly fixed to the outer surface 2F side of the first surface 2C of the bag body 2 so as to cover the opening 4 of the bag body 2.

  The method for fixing the gas permeable film 3 and the water vapor permeation prevention sheet 5 to the first surface 2C of the bag body 2 is preferably performed as follows. For example, before closing the opening of the one end 2A and the opening of the other end 2B of the bag main body 2, a heating member for heat sealing is put in the bag main body 2 and the outside of the bag main body 2 corresponds. Another heating member is arranged correspondingly. In this state, the adhesive on the four sides of the gas permeable film 3 and the adhesive on the four sides of the water vapor permeation prevention sheet 5 are applied to the inner surface 2E of the first surface 2C and the outer surface 2F of the first surface 2C, respectively. It is fixed at the same time by crimping.

  As a result, the gas permeable film 3 and the water vapor permeation prevention sheet 5 are brought into contact with the first face 2C of the bag body 2 by the first heat sealing operation. The inner surface 2E and the outer surface 2F of the surface portion 2C can be fixed in close contact with each other. That is, by one heat sealing operation, the gas permeable film 3 is fixed to the inner surface side of the first surface portion 2C of the bag body 2 so as to correspond to the opening 4 of the bag body 2 and also prevents water vapor transmission. The seat 5 is fixed to the outer surface 2F side of the first surface 2C of the bag body 2 so as to correspond to the opening 4 of the bag body 2.

  As shown in FIGS. 4 and 5, preferably, the water vapor permeation prevention sheet 5 has, for example, continuous cut portions 10 formed in advance on four sides thereof. The cut portion 10 is a half-cut groove formed from the outer surface side of the water vapor transmission prevention sheet 5 to an intermediate position in the thickness of the water vapor transmission prevention sheet 5. This cut portion 10 may be formed continuously or intermittently.

  Thereby, the water vapor permeation prevention sheet 5 has a rectangular or square peeling portion 11 which is an area inside the cut portion 10 and a frame-shaped release portion which is an area outside the cut portion 10 with the cut portion 10 as a boundary. And a remaining portion 12. This cut 10 is also shown in FIG.

  The size of the peeling portion 11 is the same as the size of the opening 4 of the bag body 2 or slightly larger than the size of the opening 4. This is for exposing the entire opening 4 when the peeling portion 11 is peeled off when the culture chamber is humidified. The frame-shaped remaining portion 12 is a portion left outside the opening 4 on the outer surface 2F of the first surface portion 2C of the bag body 2.

  As shown in FIGS. 4 and 5, the opening at one end 2A and the opening at the other end 2B of the bag body 2 are sealed by, for example, heat sealing, and the inside of the bag body 2 contains liquid as liquid. Is filled with sterilized water W. When the sterilized water W is filled in this way, the opening 4 of the bag main body 2 is completely closed by the gas permeable film 3, so that the sterilized water W in the bag main body 2 is released from the opening 4. Leakage to the outside of the bag body 2 is reliably prevented.

Moreover, since the water vapor permeation prevention sheet 5 covers the opening 4 of the bag body 2 from the outside, when the humidification bag 1 is not used, the water vapor permeation prevention sheet 5 allows the water vapor of the sterilized water W to be gas permeable. Release to the outside through the film 3 can be reliably prevented.
In the state of the final product illustrated in FIG. 5, the humidifying bag 1 illustrated in FIG. Therefore, the humidifying bag 1 is protected by the outer bag 13 shown in FIG. 5 when not in use, and the steam does not leak from the bag body 2.

  When the humidifying bag 1 humidifies the culture chamber of the incubator, as shown in FIG. 6, the operator peels off the covering sheet 6 so that the peeling portion 11 of the water vapor permeation preventing sheet 5 is put together with the peeling sheet 6. Can be removed.

  Next, a CO2 incubator 100 will be described with reference to FIG. 7 as a preferred example of an incubator in which the above-described humidifying bag 1 is disposed.

  The CO2 incubator 100 is used for culturing cultured cells requiring a high CO2 concentration by controlling the temperature and the CO2 concentration in the culture chamber 101 of the CO2 incubator 100. The CO2 incubator 100 is installed in an animal cell culture room (not shown), and the animal cell culture room has a germicidal lamp therein. A clean bench is placed next to the animal cell culture room, and the environment is such that culture can be performed immediately after aseptic operation.

  When culturing cells using the CO2 incubator 100, for example, the concentration of CO2 is 5%. The reason why a predetermined CO2 concentration, for example, 5% concentration of CO2 is used when culturing cells in this manner is as follows.

(1) Maintenance of pH As time elapses after culturing cells, the pH in the culture solution tends to be more acidic. Therefore, if sodium bicarbonate is mixed in the culture solution and a thin carbon dioxide gas is present in the incubator, a buffering action occurs and the pH is hardly fluctuated. When the culture solution becomes acidic, it is necessary to mix a substance that changes color and replace the culture solution when the color changes.

(2) Requirement of carbon dioxide Not all photosynthetic bacteria, but some photosynthetic bacteria require carbon dioxide as a carbon source.

(3) Partial pressure of carbon dioxide in arterial blood If carbon dioxide is present at 5% in the atmosphere, the atmospheric pressure is 760 mmHg, which is 5%.

  Next, a preferred structure example of a CO2 incubator to which the above-described humidifying bag 1 is applied will be described with reference to FIG.

  FIG. 7 is a longitudinal sectional view showing a preferred embodiment of a CO2 incubator to which the above-described humidifying bag 1 is applied.

  The CO2 incubator 100 shown in FIG. 7 has a rectangular parallelepiped culture chamber 101 inside. The culturing chamber 101 can uniformly control the wall surface temperature in the tank of the culturing chamber 101 by adopting a so-called water jacket heating method. In the culture room 101, dew condensation on the wall surface inside the tank, which is a hotbed of various bacteria, can be minimized by a breeze circulation system using vertical airflow.

  As shown in FIG. 7, the CO2 incubator 100 has a main body 102 and a door 103. The main body 102 and the door 103 form a metal rectangular parallelepiped box, and a culture chamber 101 is provided therein. The main body 102 has a front opening 104. The door 103 is attached to the main body 102 by a hinge, and the door 103 can open and close the front opening 104. Inside the door 103, a heater 105 and a heat insulating material (not shown) are arranged. On the front surface of the door 103, a touch panel type operation unit 106 is arranged.

  The main body 102 includes a water jacket 107. The water jacket 107 is arranged on the top surface, the bottom surface, the left and right side surfaces, and the back surface of the culture room 101. Further, a heater H is disposed on a water jacket 107 (designated by reference numeral 107A) on the bottom surface. Thus, the culture chamber 101 can uniformly control the wall surface temperature in the tank by employing a so-called water jacket method.

  A sirocco fan 108 and a CO 2 supply port 109 are provided at the back of the main body 102. When the sirocco fan 108 is operated according to a command from the control unit, in the atmosphere in the culture room 101, as shown by an arrow R, the dew condensation on the wall surface in the tank that becomes a hotbed of various bacteria is performed by a breeze circulation method using vertical airflow. Can be minimized. The CO2 supply port 109 supplies carbon dioxide from outside to the culture chamber 101 to set the CO2 concentration in the culture chamber 101 to, for example, 5%.

  As shown in FIG. 7, the water jacket 107A on the bottom has a positioning recess 120 for positioning the humidifying bag 1. The depth at which the positioning recess 120 is formed is, for example, about 1 mm to 2 mm.

  The positioning recess 120 is provided for positioning and arranging the humidifying bag 1 at the bottom of the culture chamber 101 so as not to be displaced.

  The heat generated by the heater H heats the humidifying bag 1 positioned in the positioning concave portion 120, so that the sterilized water vapor V from the humidifying bag 1 passes through the gas permeable film 3, and the culturing is performed. The release into the chamber 101 is promoted.

  Next, an example in which the worker arranges the humidifying bag 1 in the culture room 101 of the CO 2 incubator 100 shown in FIG. 7 and humidifies the inside of the culture room 101 using the steam V of the sterilized water W. Will be described.

  FIG. 6 is a schematic diagram showing a state in which the gas permeable film 3 of the bag body 2 is exposed by peeling off the covering sheet 6 from the bag body 2 and removing the peeling portion 11 of the water vapor permeation preventing sheet 5 together with the covering sheet 6. FIG.

  As shown in FIG. 5, in the humidifying bag 1 before use, the opening of the one end 2A and the opening of the other end 2B of the bag main body 2 are already sealed by, for example, heat sealing. Is filled with sterile water W in advance. Since the opening 4 of the bag body 2 is closed off by the gas permeable film 3, the sterilized water W in the bag body 2 is reliably prevented from leaking from the opening 4.

  Moreover, in the humidifying bag 1 before use, the water vapor permeation prevention sheet 5 covers the gas permeable film 3 exposed at the opening 4 of the bag body 2 from the outside, so that the water vapor of the sterilized water W is gas permeable. It is prevented from being released outside through the conductive film 3. The inside of the bag body 2 is completely sterilized with sterilized water W. The humidifying bag 1 is packaged in an outer bag 13.

  Therefore, when disposing the humidifying bag 1 in the culture chamber 101 of the CO2 incubator 100 shown in FIG. 7, the operator tears the outer bag 13 and takes out the humidifying bag 1.

  Thereafter, as shown in FIG. 6, the operator lifts the covering sheet 6 with a finger and peels it off the outer surface 2F of the first surface portion 2C of the bag main body 2 to remove the humidifying bag 1 from the CO2 incubator shown in FIG. 7. The positioning is performed in the positioning recess 120 in the 100 culture chambers 101.

  In this manner, by peeling off the covering sheet 6 shown in FIG. 6 from the outer surface 2F side of the first surface portion 2C of the bag body 2, the adhesive portion 6B of the covering sheet 6 can only remove the peeling portion 11 of the water vapor transmission preventing sheet 5. By lifting them together, they can be separated from the cut portions 10 and removed from the gas permeable film 3. As described above, since the peeling portion 11 can be removed from above the opening 4 of the bag body 2, the gas permeable film 3 covering the opening 4 of the bag body 2 can be exposed.

  As shown in FIG. 6, when the gas permeable film 3 covering the opening 4 of the bag body 2 is exposed, the sterilized water W in the bag body 2 is heated by the heater H shown in FIGS. Thus, the steaming of the sterilized water W is promoted to become steam V. The water vapor V passes through the gas permeable film 3 and is supplied into the culture chamber 101 shown in FIG.

  Then, as shown in FIG. 7, the water vapor V is circulated in the atmosphere in the culture chamber 101 by a breeze circulating method using vertical airflow as indicated by an arrow R, thereby causing human cells or animal cells to circulate. In order to culture the culture, the inside of the culture room 101 is maintained in a humidity environment of 90% or more.

  By the way, since the humidifying bag 1 can be placed in the positioning recess 120 in the culture room 101 of the CO 2 incubator 100 shown in FIG. 7, the operator can easily position the humidification bag 1 in the culture room 101. it can. Moreover, since the positioning recess 120 is provided at the bottom of the culture chamber 101, there is an advantage that the operator can easily determine where to place the humidification bag 1 in the culture chamber 101.

  By using the humidifying bag 1 having the above-described configuration, the liquid W such as water does not spill in the culture chamber 101 of the incubator 100 at all, as compared with the case of using a conventional humidifying vat. Therefore, when trying to obtain a humidity environment in the culture room 101, the handling becomes easy, and the inside of the culture room 101 can be easily humidified. Therefore, the workability of the cell culture operation by the incubator 100 can be improved.

  Further, even if an external force such as an earthquake is applied to the CO2 incubator 100, the use of the humidifying bag 1 eliminates the inconvenience of water spilling.

<Second embodiment>
Next, FIG. 8 is a perspective view showing a second embodiment of the humidifying bag of the present invention.

  The humidifying bag 1 shown in FIG. 8 has substantially the same structure as the humidifying bag 1 of the first embodiment shown in FIGS. 1 and 5, but differs in the following points.

  In the humidifying bag 1 in which the sterilized water W of the first embodiment shown in FIGS. 1 and 5 is already sealed, as shown in FIG. 5, the inside of the bag body 2 is filled with the sterilized water W in advance. Since the humidification bag 1 is a product filled with sterilized water W in advance, if the operator places the humidification bag 1 in the culture room 101, the humidification process in the culture room 101 can be performed immediately.

  On the other hand, in the case of the humidifying bag 1 of the second embodiment shown in FIG. 8, the one end 2A and the other end 2B of the bag body 2 are sealed together, and The end part 2B is provided with an injection part 20 for injecting and filling sterile water. The humidifying bag 1 is empty because the bag body 2 is not filled with sterile water in advance.

  Thereby, in the case of the humidifying bag 1 of the second embodiment shown in FIG. 8, the operator later supplies the necessary liquid, for example, sterilization, into the bag main body 2 of the humidifying bag 1 through the injection unit 20. Water W can be filled. Thereafter, if the injection part 20 is closed with the stopper 21, the humidifying bag 1 can be sealed so that the sterilized water W does not leak.

<Third embodiment>
Next, FIG. 9 is a front view showing a third embodiment of the humidifying bag of the present invention.

  The humidifying bag 1S of the third embodiment shown in FIG. 9 has substantially the same structure as the humidifying bag 1 of the first embodiment shown in FIGS. 1 and 5, but differs in the following points.

  This humidifying bag 1S has an extension 1T at the upper end. A hole 1H is formed in the extension 1T. A support rod P is arranged in the culture chamber. The extension 1T can be detachably suspended from the support bar P by using a hook 1R. By doing so, the humidifying bag 1S can be humidified from an intermediate position in the culture room so as to be suspended in the culture room. Of course, the humidification bag 1S may be placed at the bottom of the culture room without being hung.

  As described above, the humidifying bag 1 (1S) of the embodiment of the present invention is a humidifying bag for humidifying the inside of a processing device such as the incubator 100.

  The humidifying bag 1 (1S) has an opening 4 and is arranged in the bag body 2 for enclosing a liquid (for example, sterile water W) and in the bag body 2 so as to close the opening 4 of the back body 2. A gas permeable member 3 that humidifies a culture chamber 101 of an incubator 100, which is an example of the inside of the apparatus, through a liquid gas (steam of sterile water W) without passing the liquid in the bag body 2; By covering the member 3, gas is prevented from flowing out of the bag body 2 from the gas permeable member 3, and is removed when the culture chamber 101 of the incubator 100 is humidified to expose the gas permeable member 3. A transmission prevention sheet 5 is provided.

  Thereby, in the humidifying bag 1 (1S), the gas permeable member 3 does not pass through the liquid while the bag body 2 is filled with the liquid, and the gas permeation prevention sheet 5 allows the liquid to pass from the gas permeable member 3 to the liquid. The gas can be prevented from leaking into the culture room 101 of the incubator 100.

  For this reason, the humidifying bag 1 does not spill liquid such as water in the culture chamber 101 of the incubator 100 at all, and is easier to handle than using a conventional humidifying vat. Moreover, if the gas permeation prevention sheet 5 is removed, the liquid gas leaks from the gas permeable member 3, so that the inside of the culture chamber 101 can be easily humidified by the liquid gas.

  In the humidification bag 1 (1S), the humidification inside of the culture room 101 of the incubator 100 is peeled off when the humidification inside the culture room 101 of the incubator 100, so that the gas permeable prevention sheet 5 And a covering sheet 6 for removing the sheet from the sheet.

  Thus, by peeling off the covering sheet 6, both the covering sheet 6 and the gas permeation prevention sheet 5 can be easily and reliably removed from the gas permeable member 3.

  In the humidifying bag 1, the gas permeation prevention sheet 5 has a cut portion 10, and the cut portion 10 has a cut portion 10 that is removed from the gas permeable member 3 when the cover sheet 6 is peeled off, and a bag body 2 side. The remaining portion 12 is separated. Thereby, when the inside of the culture chamber 101 of the incubator 100 is humidified, the cover sheet 6 is peeled off, so that only the peeling portion 11 of the gas permeation prevention sheet 5 together with the cover sheet 6 can be easily and reliably separated from the gas permeable member 3. Can be removed.

  In the humidifying bag according to the fourth aspect, the bag body has an injecting portion for containing a liquid. Thus, when humidifying the inside of the culture chamber 101 of the incubator 100, the operator can later inject any liquid necessary for humidification into the humidification bag 1.

  An incubator provided with the humidifying bag according to claim 5 is an incubator for setting the inside to a predetermined humidity environment, and has an opening, a bag body for enclosing a liquid, and an opening of the back body. A gas permeable member that is disposed in the bag body so as to close and humidifies the inside of the incubator through a liquid gas without passing the liquid in the bag body, and a gas permeable member that covers the gas permeable member to cover the gas from the gas permeable member. A humidification bag having a gas permeation prevention sheet that prevents the gas permeation member from coming out of the bag body and removes the inside of the incubator when it is humidified to expose the gas permeable member.

  Thereby, in the incubator 100 provided with the humidifying bag 1 (1S), the gas permeable member 3 does not allow the liquid to pass through, while the bag body 2 of the humidifying bag 1 (1S) encloses the liquid. The prevention sheet 5 can prevent the liquid gas from leaking from the gas permeable member 3 into the culture room 101 of the incubator 100.

  For this reason, the humidifying bag 1 does not spill liquid such as water in the culture chamber 101 of the incubator 100 at all, and is easier to handle than using a conventional humidifying vat. Moreover, if the gas permeation prevention sheet 5 is removed, the liquid gas leaks from the gas permeable member 3, so that the inside of the culture chamber 101 can be easily humidified by the liquid gas.

  As described above, the present invention has been described with reference to the embodiments. However, each embodiment is an example, and the scope of the invention described in the claims can be variously changed without departing from the gist of the invention. .

  Although the CO2 incubator is used as the incubator using the humidifying bag according to the embodiment of the present invention, the incubator is not limited to this, and may be a multi-gas incubator.

  In the illustrated incubator 100, as a heating method, a water jacket method in which a culture chamber is heated using a heater disposed on a bottom surface and water jackets disposed on a side surface, a top surface, and a back surface is employed.

  However, not limited to this, another heating method in the incubator is a direct heat jacket method that heats the culture chamber using a heater arranged on the bottom and panel heaters arranged on the side, top and back. You may.

  As shown in FIG. 7, in the water jacket 107A on the bottom surface, the positioning concave portion 120 is provided to dispose the humidifying bag 1 at the bottom of the culture chamber 101 so as not to be displaced. However, the present invention is not limited to this, and the water jacket 107A on the bottom surface may be flat without providing the positioning recess 120.

  As shown in FIGS. 4 to 6 described above, in the humidifying bag 1 (1S), the opening 4 is formed only on the first surface 2C side of the bag body 2, and the opening 4 is formed by the gas permeable film 3. It is closed to cover. However, in the humidifying bag 1 (1S) of the embodiment of the present invention, openings are formed in both the first surface 2C and the second surface 2D of the bag body 2, and these openings are respectively formed in the gas permeable film 3 A structure may be adopted in which the cover is closed by covering with a cover.

  The opening 4 is provided on at least one of the first surface 2C and the second surface 2D of the bag body 2 according to the type of the incubator, the size of the culture room, and the contents of the culture experiment using the incubator, and the area of the opening 4 is reduced. Just set it. Depending on the type of incubator, the size of the culture room, and the contents of the culture experiment using the incubator, a large opening 4 is provided for the small-sized bag body 2 or a small opening 4 is provided for the large-sized bag body 2. It can also be provided.

  The shape of the humidifying bag 1 (1S) is not limited to the illustrated rectangular or square shape, but may be other shapes such as a circular shape, and is not particularly limited.

  The shape of the opening 4 formed in the humidifying bag 1 (1S) is not limited to the illustrated rectangular or square shape, but may be other shapes such as a circular shape, and is not particularly limited.

  As the liquid sealed in the humidifying bag 1 (1S), sterilized water W is used to make the inside of the culture chamber 101 a humidity environment of 90% or more. However, the liquid is not limited to this, and other types of water or water may be used. Alternatively, the liquid may be a different kind of liquid from water.

  Further, the humidifying bag 1 may be disposed in a tray-type vat without an upper lid, which is conventionally used, in the culture chamber 101.

DESCRIPTION OF SYMBOLS 1 Humidification bag 1S Humidification bag 2 Bag main body 3 Gas permeable film (gas permeable member)
4 Opening 5 Water vapor permeation prevention sheet (gas permeation prevention sheet)
Reference Signs List 6 covering sheet 11 peeling portion of water vapor permeation prevention sheet 12 remaining portion of water vapor permeation prevention sheet 100 incubator 101 culture room H heater

Claims (5)

A humidifying bag for humidifying the inside of the device,
A bag body having an opening and for enclosing a liquid,
Wherein disposed on the inner surface side of said bag body so as to close the opening of the bag body, the liquid in the bag body and the gas permeable member to humidify the interior of the device through the gas of the liquid without passing ,
The gas permeable member is disposed on the outer surface side of the bag body so as to close the opening of the bag body, and covers the gas permeable member to prevent the gas from flowing out of the bag body from the gas permeable member. A gas permeation prevention sheet that removes the inside of the device when humidifying and exposes the gas permeable member,
A humidifying bag comprising:   2. A coating sheet which is adhered on said gas permeation prevention sheet and is peeled off when humidifying to remove said gas permeation prevention sheet from said gas permeable member. A humidifying bag according to claim 1.   The gas permeation prevention sheet has a cut portion, and the cut portion separates a peeled portion that is removed from the gas permeable member when the cover sheet is peeled off, and a remaining portion that remains on the bag body side. The humidifying bag according to claim 2, wherein the humidifying bag is provided.   The humidifying bag according to any one of claims 1 to 3, wherein the bag body has an injection portion for injecting the liquid. An incubator provided with a humidifying bag , wherein the inside of the humidifying bag is set to a predetermined humidity environment using the humidifying bag according to any one of claims 1 to 4 .

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