JP6693731B2 - Method for screening microorganisms for defective fermentation inhibitor, method for producing defective fermentation inhibitor, and method for fermentation of biomass using defective fermentation inhibitor obtained by the same method - Google Patents

Description

  The present invention relates to a method for screening a microorganism for a defective fermentation inhibitor, a method for producing a defective fermentation inhibitor, and a method for fermentation of biomass using the defective fermentation inhibitor obtained by the method.

  BACKGROUND ART Conventionally, in a livestock house for raising livestock such as cows and pigs or a chicken house where chickens are raised (hereinafter collectively referred to as a livestock house, etc.), odor may be a problem depending on the location environment.

  In particular, in livestock shelters and the like close to residential areas, various measures are taken in order to suppress the odor generated from excretions of livestock and poultry (hereinafter collectively referred to as livestock).

  Among them, the method of spraying a predetermined deodorant material such as liquid or powder into the inside of a livestock house or the like can be carried out relatively easily over a wide range and is widely used.

  For example, Patent Document 1 describes that by spraying an extraction residue of coffee, ammonia gas generated from manure and other odorous gases can be adsorbed and the deodorizing process of manure can be effectively performed.

  Further, in Patent Document 2, a culture of filamentous fungi belonging to the genus Scopraliopsis, which has a high ability to utilize and decompose ammonia, is used to remove ammonia odor derived from organic waste such as manure of livestock and raw garbage. The method of deodorizing is described.

JP, 06-141722, A JP, 2002-086107, A

  However, even with the above-mentioned conventional malodor reducing means, a sufficient odor suppressing effect has not yet been obtained, and the practicality is poor.

  For example, in the method of spraying the extraction residue of coffee, the ability to adsorb odor is poor, and a considerable amount of extraction residue is required to exert a sufficient adsorption effect. Then, there arises a problem that a coffee odor newly generated by the extraction residue itself becomes a problem and that the volume of manure and the like increases.

  Further, according to the method using the culture of filamentous fungus, since the filamentous fungus itself proliferates, it is possible to avoid the problem that the bulk of manure and the like is extremely increased with the treatment, but the growth conditions of the filamentous fungus are relatively strict. However, there is a problem that the filamentous fungus cannot be propagated on the object to be treated unless it is in a sufficiently dry state.

  In addition, although various treatments with microorganisms have been performed so far, effective bacteria such as added odor itself giving off a bad odor during fermentation have not been obtained, and there remains a problem in suppressing odor by fermentation. Was there.

  Further, there is a demand for a microorganism capable of suppressing odor not only in excrement of livestock and the like discharged from livestock sheds and the like, but also in activated sludge secondarily produced by water treatment by the activated sludge method. In addition, not only excrement of livestock and activated sludge but also so-called waste biomass (hereinafter simply referred to as biomass) including food waste, construction waste, black liquor, sewage sludge, garbage, etc. Microorganisms capable of suppressing odor are desired.

  The present invention has been made in view of such circumstances, the odor generated from the biomass, it is possible to reduce by fermentation without being relatively trapped in the water content, moreover, by suppressing the growth of malodor producing bacteria. Provided is a method for screening a microorganism for a poor fermentation inhibitor capable of preventing poor fermentation.

  The present invention also provides a method for producing the poor fermentation inhibitor and a method for fermenting biomass using the poor fermentation inhibitor.

  In order to solve the above conventional problems, in the microorganism screening method for the poor fermentation inhibitor according to the present invention, 0.6 ± 0.1 parts by weight of dry Stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, 0.6 ± 0.1 Part by weight of dried okara powder, 12 ± 1 part by weight of brackish water containing photosynthetic bacteria, a standing step for standing for a predetermined period of time, and a predetermined amount of the mixed solution after the standing step. Weakly sterilized in a container that is 10 cm or more from any position on the outer surface of the contained shape of the mixed liquid and 17 cm or less from at least any position on the outer surface. A container containing step for forming a region, a predetermined container containing the mixed solution through the container containing step is placed in a heating space, and the heating space is heated to about 2.5 atm from 150 to 160 ° C. at about 2.5 atm. It takes 45 to 60 minutes to reach the state, and the temperature is 150 to 160 ° C at approximately 2.5 atmospheric pressure. Hold for 1 to 3 minutes, then set the temperature of the heating space to 115 ~ 125 ℃ at about 2 atm for 3 to 5 minutes, and set the temperature down to 115 ~ 125 ℃ at about 2 atm for 20 to 40 minutes. Furthermore, the heating space is cooled to room temperature and normal pressure for 24 to 30 hours to lower the temperature, and the microorganism selection step of leaving the heat-selected microorganisms in the mixed solution is performed.

  Further, in the method for producing a poor fermentation inhibitor according to the present invention, 0.6 ± 0.1 parts by weight of dry Stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, 0.6 ± 0.1 parts by weight of dry okara powder, photosynthesis 12 ± 1 part by weight of brackish water containing bacteria, a static step of leaving a mixed solution for a predetermined time, and the mixed solution that has passed through the static step is stored in a predetermined container, and the mixed solution is stored. In 10 cm or more from any position on the outer surface of the containing shape of the same mixed solution, and a container containing step of forming a weak sterilization area that is 17 cm or less from at least any position on the outer surface, Place a predetermined container containing the mixed solution in the heating space after the container storage process, and raise the heating space from room temperature and normal pressure to 150 to 160 ° C at about 2.5 atm for 45 to 60 minutes. Warm up and maintain the condition of 150-160 ℃ at about 2.5 atmosphere for 1-3 minutes, and then heat the heating space at about 2 atmosphere. The temperature is set to 115-125 ℃ for 3-5 minutes, the temperature is kept at 115-125 ℃ for 20-40 minutes at about 2 atm, and the heating space is kept at room temperature and normal pressure for 24-30. The temperature is lowered over time, a microorganism selection step of leaving the selected microorganisms heated in the mixed solution, and at least the mixed solution that has undergone the microorganism selection step is solid-liquid separated by means capable of transferring to the liquid phase. A solid-liquid separation step of obtaining a microorganism-containing liquid, a sugar source is added to the obtained microorganism-containing liquid and fermented at room temperature and atmospheric pressure for a predetermined time, the pH of the microorganism-containing liquid is 4.5 or less, and the redox potential is- The first fermentation step to 100 mV or less, and the fermentation solution of Stevia stalk is added to the microorganism-containing solution that has undergone the first fermentation step, and fermented until the pH becomes 3.1 or less And a fermentation step.

  Further, in the method for producing a poor fermentation inhibitor according to the present invention, the stevia stalk ripening solution is prepared by adding 10 ± 2 parts by weight of water to 1 part by weight of dry stevia stalk powder. Stevia stalk dispersion preparation step to prepare a, Stevia stalk dispersion obtained through the Stevia stalk dispersion preparation step is stored in a predetermined container, and the same Stevia stalk dispersion is stored in the stored Stevia stalk dispersion. 10 cm or more from any position on the outer surface of the shape, and, and a stevia stalk dispersion liquid container accommodating step of forming a weak sterilization region that is 17 cm or less from at least any position on the outer surface, and the stevia stalk A predetermined container containing Stevia stalk dispersion liquid is placed in the heating space through the dispersion liquid container storing step, and the heating space is heated from room temperature and atmospheric pressure to about 2.5 atm to 150 to 160 ° C until 30 to 40 ° C. It takes 15 minutes to heat up at 2.5 bar Maintain the condition of 0-160 ℃ for 1-3 minutes, then set the temperature of heating space to 115-125 ℃ at about 2 atm for 3-5 minutes, and set the temperature to 115-125 ℃ at about 2 atm. Maintained for 20-40 minutes, by further cooling to 85-95 ° C., Stevia stem-derived microorganism selection step of leaving the dried Stevia stem-derived microorganisms heat-selected in the Stevia stem dispersion, and 85- Stevia stalk containing microorganisms derived from Stevia stalk with a liquid phase Brix value of 2.0 to 4.0 by solid-liquid separation of at least the Stevia stalk dispersion cooled to 95 ° C by means capable of transferring microorganisms to the liquid phase. Stevia stalk extract preparation step to obtain the extract of, the Stevia stalk extract is heated and boiled down and then cooled, Brix value 4.0 ~ 7.0 pH 6.0 or less, ORP is 10 ~ 99mV Stevia stalk concentrate. And a step of concentrating the stevia stem concentrate at room temperature. , Having the characteristics also pH is the ORP at 5.0 or less is prepared through the ripening step to obtain the following Stevia stems ripening liquid -100 mV, the.

  Further, in the method for fermenting biomass according to the present invention, 1 part by weight of the defective fermentation inhibitor obtained by the method for producing a defective fermentation inhibitor according to claim 2 or 3 is diluted with a predetermined amount of water to be diluted. A liquid is prepared, and this diluted liquid is added to 8000 to 10000 parts by weight of biomass to give a poor fermentation inhibitor mixing step of adjusting the water content to 65 to 70%, and the biomass that has gone through the bad fermentation inhibitor mixing step is used as a surface layer. From 40 to 50 cm, the first biomass fermentation process, which is allowed to stand in a stacked state until the internal temperature reaches 55 ° C, and the biomass that has undergone the first biomass fermentation process is cut back to a temperature that does not exceed 75 ° C. A second biomass fermentation process that performs aerobic fermentation of the biomass until the moisture content of the biomass is 40% or less, a sieving process that sifts the biomass that has undergone the second biomass fermentation process, and a sieving process. Work And the aging step in which the aged biomass is accumulated and allowed to stand until the water content becomes 20% or less.

  According to the method for screening a microorganism for poor fermentation inhibitor according to the present invention, 0.6 ± 0.1 parts by weight of dry Stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, and 0.6 ± 0.1 parts by weight of dried okara powder. , 12 ± 1 part by weight of brackish water containing photosynthetic bacteria, a standing step of leaving the mixed solution to stand for a predetermined time, and the mixed solution which has passed through the standing step was housed in a predetermined container and stored. A container containing step of forming a weak sterilization area that is 10 cm or more from any position on the outer surface of the mixed solution containing shape of the mixed solution, and is 17 cm or less from at least any position on the outer surface. , A predetermined container containing the mixed solution through the container storing step is placed in a heating space, and the heating space is heated from room temperature and atmospheric pressure to about 2.5 atm to 150 to 160 ° C for 45 to 60 minutes. The temperature is raised to about 2.5 atm, and the temperature is maintained at 150 to 160 ° C for 1 to 3 minutes. The temperature of the space is set to 115-125 ℃ at about 2 atm for 3 to 5 minutes to set the temperature down, and the temperature of 115-125 ℃ is maintained at about 2 atm for 20 to 40 minutes. The temperature is lowered to 24 to 30 hours to reach the state, and the microorganism selection step of leaving the selected microorganisms in the mixed solution by heating is performed, so that the odor generated from the biomass is relatively trapped in the water content. It is possible to provide a method for screening a microorganism for a defective fermentation inhibitor, which can be reduced by fermentation without fermentation, and which can prevent the fermentation of malodor producing bacteria and prevent defective fermentation.

  Further, according to the method for producing a poor fermentation inhibitor according to the present invention, 0.6 ± 0.1 parts by weight of dry stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, and 0.6 ± 0.1 parts by weight of dried okara powder. , 12 ± 1 part by weight of brackish water containing photosynthetic bacteria, a standing step of leaving the mixed solution to stand for a predetermined time, and the mixed solution which has passed through the standing step was housed in a predetermined container and stored. A container containing step of forming a weak sterilization area that is 10 cm or more from any position on the outer surface of the mixed solution containing shape of the mixed solution, and is 17 cm or less from at least any position on the outer surface. , A predetermined container containing the mixed solution through the container storing step is placed in a heating space, and the heating space is heated from room temperature and atmospheric pressure to about 2.5 atm to 150 to 160 ° C for 45 to 60 minutes. The temperature is raised at about 2.5 atmospheres and the temperature is maintained at 150-160 ℃ for 1-3 minutes. The temperature is set to 115-125 ° C over 3-5 minutes to set the temperature down, and the temperature is maintained at 115-125 ° C for 20-40 minutes at about 2 atm, and the heating space is kept at room temperature and normal pressure. ~ 30 hours to lower the temperature, a microorganism selection step of leaving the selected microorganisms in the mixed solution by heating, and at least solid-liquid separation of the mixed solution that has undergone the microorganism selection step by means capable of transferring the microorganisms to the liquid phase A solid-liquid separation step of obtaining a microorganism-containing liquid by adding a sugar source to the obtained microorganism-containing liquid and fermenting for a predetermined time at room temperature and atmospheric pressure, the pH of the microorganism-containing liquid is 4.5 or less, and the redox potential Fermentation step to make the -100 mV or less, and fermented Stevia stalk ripening solution to the microorganism-containing liquid that has undergone the first fermentation step and ferment until the pH becomes 3.1 or less Since the fermentation step of 2 is included, the odor generated from the biomass is It is possible to provide a method for producing a poor fermentation inhibitor which can be reduced by fermentation without being relatively concerned with the content, and which can prevent bad fermentation by suppressing the propagation of malodor producing bacteria.

  The Stevia stem ripening solution is a Stevia stem dispersion liquid preparation step of preparing a Stevia stem dispersion liquid by adding 10 ± 2 parts by weight of water to 1 part by weight of dry Stevia stem powder. Stevia stem dispersion obtained through the stem dispersion preparation step is stored in a predetermined container, and 10 cm or more from any position on the outer surface of the storage shape of the stevia stem dispersion stored in the stored stevia stem dispersion. And, and the Stevia stem dispersion liquid container accommodation step of forming a weak sterilization area that is 17 cm or less from at least any position on the outer surface, and the Stevia stem dispersion liquid is accommodated through the Stevia stem dispersion liquid container accommodation step. The predetermined container is placed in the heating space, and the heating space is heated from room temperature and normal pressure to 150 to 160 ° C at about 2.5 atm for 30 to 40 minutes, and at about 2.5 atm to 150 to 160 ° C. Maintain 160 ℃ for 1-3 minutes, then add The temperature of the space is set to 115-125 ℃ at 2 atm for 3-5 minutes, and the temperature is kept at 115-125 ℃ for 20-40 min at 2 atm, and then cooled to 85-95 ℃. By performing a Stevia stalk-derived microorganism selection step of leaving the dried Stevia stalk-derived microorganisms that have been heat-selected in the Stevia stalk dispersion, at least the microorganisms in a liquid phase of the Stevia stalk dispersion cooled to 85 to 95 ° C. Solid-liquid separation by means that can be transferred to, a Stevia stalk extract preparation step to obtain an extract of Stevia stalk containing a Stevia stalk-derived microorganism in a liquid phase Brix value of 2.0 to 4.0, and the Stevia stalk The extract is heated and boiled down and then cooled, the Brix value is 4.0 to 7.0, the pH is 6.0 or less, and the ORP is 10 to 99 mV. Aged, and stevia stem ripening with pH less than 5.0 and ORP less than -100mV A ripening step of obtaining a liquid, and if it is prepared through the steps, a better Stevia stem ripening liquid can be prepared, and a method capable of producing a poor fermentation inhibitor that can function more steadily is provided. be able to.

  According to the method for fermenting biomass according to the present invention, 1 part by weight of the poor fermentation inhibitor obtained by the method for producing a poor fermentation inhibitor according to claim 2 or 3 is diluted with a predetermined amount of water. A dilute solution is prepared by adding the diluted solution to 8000 to 10000 parts by weight of biomass to prepare a poor fermentation inhibitor mixing step for adjusting the water content to 65 to 70%, and a biomass subjected to the poor fermentation inhibitor mixing step. , The first biomass fermentation process in which it is left in a piled state until the temperature inside the surface layer reaches 40 to 50 cm reaches 55 ° C, and the biomass that has passed through the first biomass fermentation process is cut back and does not exceed 75 ° C. A second biomass fermentation step of performing aerobic fermentation of the biomass until the moisture content of the biomass becomes 40% or less while maintaining the temperature; and a sieving step of sieving the biomass that has undergone the second biomass fermentation step while unraveling the biomass. Sieving Since the biomass that has undergone the mashing process has been accumulated and the aging process in which the biomass is allowed to stand until the water content falls to 20% or less, the odor generated from the biomass can be reduced by fermentation without being restricted by the water content. In addition, it is possible to provide a method for fermenting biomass, which can prevent the fermentation of malodor-producing bacteria and prevent defective fermentation, and can obtain a fermented product that can be used as a fertilizer.

  The present invention, the odor generated from the biomass, can be reduced by fermentation without being relatively trapped in the water content, moreover, of the microorganisms for poor fermentation inhibitors that can prevent the bad fermentation by suppressing the growth of malodor producing bacteria. A screening method is provided.

  The biomass to be fermented here is not particularly limited, and for example, not only excrement of livestock and activated sludge, but also pruned branches, food waste, construction waste, black liquor, sewage sludge, food waste, etc. The so-called waste-based biomass including can be used.

  The method for screening a microorganism for a poor fermentation inhibitor according to the present embodiment includes a stationary step, a container accommodation step, and a microorganism selection step.

  In the stationary step, a material as an extraction raw material for the extract contained in the poor fermentation inhibitor (hereinafter also collectively referred to as an extract extraction material) and brackish water containing photosynthetic bacteria are mixed and allowed to stand still. This is the process of eluting the extract in brackish water.

  In particular, in the microorganism screening method for the poor fermentation inhibitor according to the present embodiment, as an extract extraction material, 0.6 ± 0.1 parts by weight of dry stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, and 0.6 ± 0.1 parts by weight of Dried okara powder is used.

  In addition, it is desirable that lactic acid bacteria derived from plants adhere to at least one of these extract extraction materials. Examples of this plant-derived lactic acid bacterium include Lactobacillus plantarum and Lactobacillus buchneri. In particular, when using the extract extract material adhered Lactobacillus buchneri, by the formation of the weak sterilization region described later, it is possible to obtain a mixed liquid Lactobacillus buchneri survive the microbial selection step, also within the weak sterilization region. Propagation of microorganisms such as existing fungi, molds and yeasts that cause poor fermentation is effectively suppressed by the metabolites of Lactobacillus buchneri, such as lactic acid, acetic acid, and propanediol, thus stabilizing the poor fermentation inhibitor. Manufacturing can be made more robust.

  Brackish water containing photosynthetic bacteria is preferably mixed in an amount of 12 ± 1 part by weight with respect to each of the extract material of 0.6 ± 0.1 part by weight.

  As photosynthetic bacteria contained in this brackish water, for example, Rhodospirillum genus called purple non-sulfur bacteria, Chromatium genus called red sulfur bacterium, and Chlorobium genus called green sulfur bacterium can be used. It is desirable to use a group of photosynthetic bacteria belonging to the genus Chromatium called.

  Then, in the stationary step, the extract extraction material and brackish water containing photosynthetic bacteria are mixed in predetermined amounts, and the mixture is allowed to stand, for example, at room temperature for a predetermined time. The time required for standing may be, for example, 15 to 20 days.

  In the container accommodation step, the mixed liquid that has undergone the stationary step is accommodated in a predetermined container. The material and shape of the container to be stored are not particularly limited, but the characteristic of this step is that a weak sterilization area can be formed so that complete sterilization is not performed when heated in the microorganism selection step described later. Store in a simple container.

  This weak sterilization area is 10 cm or more from any position on the outer surface of the solid and three-dimensional shape of the liquid mixture contained in a predetermined container (hereinafter, simply referred to as an accommodation shape), and The inner area is 17 cm or less from at least one position on the outer surface.

  Here, if the storage shape is less than 10 cm from any outer surface and a shape that does not form a weak sterilization area, it is completely sterilized by the heat applied in the microorganism selection step described below, and the required bacteria Can no longer be selected.

  In addition, in the shape of the accommodation shape, where the area exceeding 17 cm is formed from any position on the outer surface, the area where the heat required for selection does not reach the deep part of the mixed liquid (non-selected area) Since it is formed, there is a possibility that many unnecessary bacteria may be mixed, so it is not always necessary to exclude the formation of the non-selection region, but the volume of this non-selection region is smaller than the volume of the weak sterilization region. It is desirable to do.

  By storing the mixed solution in a container capable of forming a weak sterilization region, more preferably in a container capable of forming a weak sterilization region without forming a non-selected region, unnecessary microorganisms (causing poor fermentation) are eliminated. However, it is possible to select the microorganisms that promote good fermentation, that is, the above-mentioned lactic acid bacteria and photosynthetic bacteria.

  The microorganism selection step is a step of selecting a lactic acid bacterium or a photosynthetic bacterium that promotes favorable fermentation by heating the mixed solution, and is specifically composed of a first heating step, a second heating step, and a temperature lowering step. It

  In the first heating step, a predetermined container containing the mixed liquid is placed in the heating space, and the heating space is heated from room temperature and normal pressure to about 2.5 atm to 150 to 160 ° C. in 45 to 60 minutes. The temperature is raised at about 2.5 atm and maintained at 150 to 160 ° C for 1 to 3 minutes.

  As the heating space in the first heating step and the second heating step described later, for example, a sterilization target accommodation space of an autoclave or a heat treatment space of a pressure cooker can be used.

  In the second heating step, following the first heating step, the temperature of the heating space is set to about 115 to 125 ° C at about 2 atm for 3 to 5 minutes, and the temperature is set to about 115 to 125 ° C at about 2 atm. For 20-40 minutes.

  Then, in the temperature lowering step, subsequent to the second heating step, the temperature of the heating space is lowered to room temperature and normal pressure for 24 to 30 hours.

  In this way, by performing the microorganism selection step including the first heating step, the second heating step, and the temperature lowering step, the microorganisms such as lactic acid bacteria and photosynthetic bacteria that have been heat-selected in the mixed solution are left behind, resulting in defective fermentation. It is possible to screen for microorganisms for inhibitors.

  The present application also provides a method for producing a poor fermentation inhibitor. In particular, it can be said that the method for producing a poor fermentation inhibitor according to the present embodiment utilizes the microorganism obtained by the method for screening a microorganism for a poor fermentation inhibitor described above.

  Specifically, the method for producing a poor fermentation inhibitor according to this embodiment includes a stationary step, a container accommodation step, a microorganism selection step, a solid-liquid separation step, a first fermentation step, and a second fermentation step. And a fermentation process. Incidentally, the stationary step and the container accommodation step in the method for producing a defective fermentation inhibitor according to the present embodiment, the microorganism selection step is the same as the same step in the method for screening a microorganism for a defective fermentation inhibitor described above, and therefore the description is omitted. To do.

  The solid-liquid separation step in the method for producing a poor fermentation inhibitor according to the present embodiment is a step of solid-liquid separating the mixed solution that has undergone the microorganism selection step to obtain a liquid phase.

  Here, the solid-liquid separation does not necessarily have to completely remove the solid content, and is an image to the extent that the solid content is roughly removed while transferring the selected microorganisms to the liquid phase to improve the fluidity. Such solid-liquid separation can be performed, for example, by storing the mixed liquid in a cloth bag or the like and utilizing the dehydrating function of the washing machine. The solid-liquid separation may be carried out by applying a centrifugal force to the extent that microorganisms can be left in the liquid phase by using a general solid-liquid separation device. The liquid phase may be obtained by soup.

  That is, in the present solid-liquid separation step, the mixed solution which has undergone the microorganism selection step is subjected to solid-liquid separation at least by means capable of transferring microorganisms to the liquid phase to obtain a microorganism-containing liquid.

  The first fermentation step is a step of adding a sugar source to the obtained microorganism-containing liquid and performing fermentation at room temperature and atmospheric pressure for a predetermined time. The sugar source is not particularly limited, and it is possible to use glucose, sucrose, white sugar, liquid sugar, honey, maple syrup, oligosaccharide, beet sugar, etc. Among them, maple syrup contains the obtained microorganism. A component that promotes growth is contained in the useful microorganism group contained in the liquid, and also when the maple syrup is actually used in the study of the present inventors, the useful microorganism group to be proliferated is positively increased. It is desirable because the results that can be obtained have been obtained. The addition amount of these sugar sources can be 0.011 ± 0.006 parts by weight, for example, per 1 part by weight of the microorganism-containing liquid.

  This first fermentation step is performed until the pH of the microorganism-containing liquid is 4.5 or lower and the redox potential is -100 mV or lower.

  The second fermentation step is a step of performing fermentation by adding the Stevia stem ripening solution to the microorganism-containing solution that has undergone the first fermentation step. The addition amount of the Stevia stem ripening liquid to the microorganism-containing liquid can be about 0.38 to 0.4 parts by weight with respect to 0.38 parts by weight of the microorganism-containing liquid. This second fermentation step is carried out until the pH is below 3.1.

  Then, by passing through the second fermentation step, the obtained microorganism-containing liquid can be used as a poor fermentation inhibitor.

  Here, the ripening solution of Stevia stalk used in the second fermentation step was obtained by immersing Stevia stalk in water or the like to extract an extract and fermenting with Stevia stalk-derived microorganisms. A fermentation broth (hereinafter, also referred to as a Stevia stem simple ripening liquid) can also be used.

  However, Stevia stem ripening liquid, Stevia stem dispersion preparation step, Stevia stem dispersion container storage step, Stevia stem-derived microorganism selection step, Stevia stem extract preparation step, extract concentration step, ripening step It is more desirable to prepare via

  The stevia stalk dispersion preparation step is a step of adding water to the powder of the dry stevia stalk to prepare a dispersion of the stevia stalk, and specifically, 10 parts by weight ± 1 part by weight of the dry stevia stalk powder. Prepare by adding about 2 parts by weight of water.

  Stevia stalk dispersion container storage step is stored in a container capable of forming a weakly sterilized region so that complete sterilization is not performed when heated in the Stevia stalk-derived microorganism selection step described below, substantially similar to the container storage step described above. It is a process.

  The weak sterilization region in the Stevia stem dispersion liquid container accommodation step is 10 cm or more from any position on the outer surface of the accommodation shape of the Stevia stem dispersion liquid contained in a predetermined container, and on the outer surface. 17 cm or less from at least one of the positions. More preferably, a container capable of forming a weak sterilization region without forming a non-selection region exceeding 17 cm from any position on the outer surface is used.

  The Stevia stalk-derived microorganism selection step is also a step of selecting Stevia stalk-derived microorganisms by heating the Stevia stalk dispersion, like the above-described microbial selection step, and specifically, the first Stevia stalk heating step and the second step. And Stevia stem heating process.

  In the first Stevia stalk heating step, a predetermined container containing the Stevia stalk dispersion liquid is placed in the heating space through the Stevia stalk dispersion container storing step, and the heating space is kept at room temperature and pressure from about 2.5 atm. The temperature is raised to a state of 150 to 160 ° C over 30 to 40 minutes, and the state of 150 to 160 ° C is maintained at about 2.5 atmospheric pressure for 1 to 3 minutes.

  In the second Stevia stalk heating step, subsequent to the first Stevia stalk heating step, the temperature of the heating space is set to 115 to 125 ° C. at about 2 atm for 3 to 5 minutes, and the temperature is set to about 2 atm to 115 to 125 ° C. Maintain at 125 ℃ for 20-40 minutes.

  And after passing through this 1st Stevia stalk heating process and a 2nd Stevia stalk heating process, by cooling to 85-95 degreeC, the dry stevia stalk derived from the heat selected in the Stevia stalk dispersion liquid is derived. Let the microorganisms remain.

  The Stevia stalk extract preparation step is a step of obtaining a liquid phase by solid-liquid separation from the Stevia stalk dispersion liquid that has undergone the Stevia stalk-derived microorganism selection step.

  Specifically, following the above-mentioned second Stevia stalk heating step, the Stevia stalk dispersion liquid cooled to 85 to 95 ° C is subjected to solid-liquid separation at least by means capable of transferring microorganisms to the liquid phase, As an extract of Stevia stem. This Stevia stem extract has a Brix value of 2.0 to 4.0 and contains microorganisms derived from Stevia stem.

  The extract concentration step is a step in which the stevia stem extract is heated and boiled down to be concentrated to a predetermined concentration to obtain a stevia stem concentrate. Specifically, Stevia stalk extract is heated and boiled down, and concentrated to a Brix value of 4.0 to 7.0, a pH of 6.0 or less, and an ORP of 10 to 99 mV when cooled to about room temperature.

  The ripening step is a step of aging the Stevia stalk concentrated solution at room temperature with fermentation to obtain a Stevia stalk ripening solution having a pH of 5.0 or less and an ORP of -100 mV or less.

  In this way, the Stevia stalk dispersion preparation step, the Stevia stalk dispersion container accommodation step, the Stevia stalk-derived microorganism selection step, the Stevia stalk extract preparation step, the extract concentration step, and the aging step, Thus, it is possible to obtain a ripening solution of Stevia stalk suitable for producing a poor fermentation inhibitor.

  The present application also provides a method for fermenting biomass using the poor fermentation inhibitor obtained by the method for producing a poor fermentation inhibitor described above.

  In particular, according to the method for fermenting biomass according to the present embodiment, it is possible to ferment biomass while suppressing the generation of malodor as much as possible, and depending on the type of biomass, the fermented product obtained may be used as a high-quality fertilizer or the like. It is also possible to use as.

  The biomass fermentation method according to the present embodiment includes a defective fermentation inhibitor mixing step, a first biomass fermentation step, a second biomass fermentation step, a sieving step, and an aging step.

  The defective fermentation inhibitor mixing step is a step of adjusting the water content to a predetermined level while adding and mixing the defective fermentation inhibitor obtained by the above-mentioned method for producing a defective fermentation inhibitor to biomass.

  The mixing ratio of the bad fermentation inhibitor and the biomass is preferably 8000 to 10000 parts by weight for 1 part by weight of the bad fermentation inhibitor, but the bad fermentation inhibitor for a large amount of biomass. In order to adjust the water content while mixing it uniformly, add 1 part by weight of the bad fermentation inhibitor to the amount of water required to make the water content of 8000 to 10000 parts by weight of biomass 65 to 70%. It is desirable to prepare a diluted solution of the poor fermentation inhibitor and add this diluted solution uniformly while cutting and stirring the biomass.

  The first biomass fermentation process is a process in which the biomass that has been subjected to the poor fermentation inhibitor mixing process is allowed to stand in a piled state to mainly perform anaerobic fermentation. This first biomass fermentation step is performed until the temperature of 40 to 50 cm from the surface layer of the deposited biomass reaches 50 to 55 ° C, preferably about 55 ° C, due to the heat of fermentation. In the first biomass fermentation step, fermentation is performed by anaerobic microorganisms in the biomass, and generally, a sulfur-containing compound metabolized by the anaerobic microorganisms causes a bad odor. According to the biomass fermentation method of the present invention, the malodor is suppressed because the poor fermentation inhibitor is mixed with the biomass.

  The second biomass fermentation process is a process of cutting back the biomass that has undergone the first biomass fermentation process and promoting aerobic fermentation while introducing oxygen into the biomass.

  In this second biomass fermentation step, the aerobic fermentation of the biomass is performed until the moisture content of the biomass becomes 40% or less while maintaining the temperature not exceeding 75 ° C. In addition, the biomass is heated at a predetermined timing so that the temperature of the biomass due to the heat of fermentation does not exceed 75 ° C., and the fermentation is performed while cooling and supplying air.

  The second biomass fermentation step is performed until the moisture content of the biomass is reduced to 30 to 40%, preferably about 40%, and more preferably 70% even when water is added so that the moisture content is 50%. Do not increase the temperature above 0 ° C again.

  The sieving step is a step of sieving the biomass that has undergone the second biomass fermentation step while unraveling it. This step is performed in order to unravel the biomass into a state suitable for fertilizer and to remove relatively large pieces of wood.

  The aging step is a step in which the biomass that has been subjected to the sieving step is placed in a piled state and allowed to stand until the water content becomes 20% or less. In this aging step, filamentous fungi grow on the surface of the deposited biomass, and further fermentation is performed.

  As described above, according to the method for fermenting biomass according to the present embodiment, since the steps described above are performed, the odor generated from biomass can be reduced by fermentation without being relatively trapped in the water content, and The fermentation of malodor producing bacteria can be suppressed to prevent defective fermentation.

  Further, the obtained fermented biomass does not give off a bad odor even when fertilizing fields such as when the biomass is excrement of livestock or activated sludge, and is a very useful fertilizer for plants. Can be used as

  Hereinafter, the method for screening a microorganism for a poor fermentation inhibitor, the method for producing a bad fermentation inhibitor, and the method for fermenting biomass according to the present embodiment will be specifically described with reference to Examples.

[1. Screening of microorganisms for defective fermentation inhibitors]
In a 20 L stainless steel container (32 cm in diameter and 38 cm in height), 0.85 ± 0.15 kg of dry stevia stalk powder, 0.85 ± 0.15 kg of rice bran powder, and 0.85 ± 0.15 kg of dry okara powder were put, Furthermore, 17 ± 1.5 kg of brackish water collected in the brackish water area of the Abu River estuary in Yamaguchi Prefecture was added and mixed with stirring to prepare several batches, which were left to stand overnight (about 15 to 17 hours) at room temperature (static. Setting process). As a result of microbiological examination of the mixture left to stand, it was confirmed that at least Lactobacillus buchneri was present among some of the detected microbes.

Separately, the collected brackish water was subjected to a microbial test to confirm that the brackish water contained 2.5 × 10 ⁵ cfu / ml of photosynthetic bacteria belonging to the genus Chromatium.

  Next, 20 L of the mixed solution that had been subjected to the stationary step was stored in a stainless steel container P1 (a cylindrical shape having an inner diameter of 28 cm and a height of 33 cm) with a capacity of about 20.31 L to form a weak sterilization area in the container (container housing Process). Similarly, in a stainless container Q1 with a cap of about 21.04 L (cylindrical with an inner diameter of 20 cm and a height of 67 cm), 20.41 L of the mixed solution that had been allowed to stand was stored and mixed in the stainless container Q1. The weakly sterilized region was formed by using a liquid containing shape of a column having a diameter of 20 cm and a height of 65 cm. At the same time, 30.85 L of the mixed solution that had been subjected to the stationary step was stored in a stainless steel container R1 with a lid (a cylindrical shape having an inner diameter of 34 cm and a height of 36 cm) with a capacity of about 32.67 L, and the mixed solution stored in the stainless steel container R1 A weak sterilization area was formed by forming the storage shape into a cylindrical shape having a diameter of 34 cm and a height of 34 cm.

  Next, each stainless steel container P1, Q1, R1 is stored in a pressure cooker together with the stainless steel containers P1, Q1, R1 and heated up to a temperature of 150 to 160 ° C. for about 45 to 60 minutes at about 2.5 atm and about 2.5 atm. The state of 150 to 160 ° C. was maintained for 1 to 3 minutes (first heating step).

  Next, subsequently, the temperature was lowered to 115 to 125 ° C at about 2 atm for 3 to 5 minutes, and the state of 115 to 125 ° C was maintained at about 2 atm for 20 to 40 minutes (second heating step). ).

  Next, the heating switch of the pressure cooker was turned off, and the temperature was lowered for 24 to 30 hours until the internal temperature of the pressure cooker became normal temperature and normal pressure (cooling step).

  Then, the lid of the pressure cooker was opened to obtain a mixed solution in which the microorganisms for defective fermentation inhibitors were screened in the stainless steel containers P1, Q1, R1. As a result of microbiological examination of this mixed solution, the presence of at least Lactobacillus buchneri was confirmed among the several microbes detected.

[2. Preparation of Stevia Stem Ripening Solution]
First, 10 ± 2 kg of water was added to 1 kg of dry Stevia stalk powder in a predetermined container and mixed uniformly to prepare a Stevia stalk dispersion (Stevia stalk dispersion preparation step). The Stevia stem dispersion was prepared in several batches.

  Next, in a stainless container P2 with a lid of about 12.27 L capacity (inner diameter 25 cm and height 25 cm), 12 L of stevia stalk dispersion prepared in the Stevia stalk dispersion preparation step was stored, and the stevia stalk stored in the container A weakly sterilized area was formed in the dispersion (stevia stem dispersion container accommodation step). Similarly, a stainless steel container Q1 with a cap of about 21.04 L (cylindrical with an inner diameter of 20 cm and a height of 67 cm) containing 12 L of the stevia stalk dispersion liquid prepared in the step of preparing the stevia stalk dispersion liquid was used. A weak sterilization region was formed by making the storage shape of the Stevia stalk dispersion liquid stored in the inside into a cylindrical shape with a diameter of 20 cm and a height of 38 cm. In addition, 30.85 L of the Stevia stem dispersion liquid prepared in the Stevia stem dispersion liquid preparation step is stored in a stainless steel container R1 with a lid of about 32.67 L capacity (cylindrical shape with an inner diameter of 34 cm and a height of 36 cm). The stevia stalk dispersion liquid was stored in a cylindrical shape having a diameter of 34 cm and a height of 34 cm to form a weakly sterilized region.

  Next, in a pressure cooker, the Stevia stalk dispersion is stored together with the stainless steel containers P2, Q1 and R1 and heated at about 2.5 atm to 150 to 160 ° C. for 30 to 40 minutes to about 2.5 atm. The temperature of 150 to 160 ° C was maintained for 1 to 3 minutes (first stevia stem heating step).

  Next, subsequently, the temperature was set to about 115 to 125 ° C at about 2 atm for 3 to 5 minutes, and the temperature was set to about 115 to 125 ° C at about 2 atm for 20 to 40 minutes (second stevia). Stem heating step).

  Next, the heating of the pressure cooker was completed, and when the pressure cooker's inside became about normal pressure (the pressure at which the lid could be opened) and reached about 85 to 95 ° C, the stainless steel containing Stevia stem dispersion was stored. The containers P2, Q1 and R1 were taken out from the pressure cooker, and the Stevia stem dispersion liquid was transferred into a cotton cloth bag.

  The cloth bag containing the Stevia stem dispersion liquid was subjected to a dehydrator while being hot, and solid-liquid separation was performed to obtain a Stevia stem extract liquid (Stevia stem extract preparation step). This Stevia stalk extract has a Brix value of 2.0 to 4.0, and a microorganism of the genus Lactobacillus, which is a Stevia stalk-derived microorganism, is contained in any of the stainless steel containers P2, Q1, and R1 by a separate microbial test. Was confirmed.

  Next, the obtained Stevia stalk extract was placed in a heat-resistant predetermined container, placed on a gas stove, and boiled down while heating for about 4 to 5 hours to prepare a Stevia stalk concentrate (extraction). Liquid concentration step). After that, when heating was terminated and the mixture was allowed to cool to room temperature and then subjected to a physicochemical test on the Stevia stem concentrate, it was confirmed that the Brix value was 4.0 to 7.0, the pH was 6.0 or less, and the ORP was 10 to 99 mV. ..

  Next, the obtained Stevia stalk concentrated liquid was stored in a fermentation aging can (12 L capacity) with a hook made of stainless steel, and allowed to stand in a room temperature environment for aging (aging step). During aging, gas with fermentation odor was generated from the Stevia stem concentrated liquid in any of the stainless steel containers P2, Q1 and R1, and the fermentation was about the same regardless of the difference in the shape of the stainless steel containers P2, Q1 and R1. It was confirmed that it was accompanied by.

  Then, when the stevia stem concentrated liquid during ripening had a pH of 5.0 or lower and an ORP of -100 mV or lower, the stevia stem ripening liquid was used. Also at this time, no difference such as abnormal fermentation due to the container shape of the stainless steel containers P2, Q1 and R1 was observed, and in the subsequent experiments, the Stevia stem ripening solutions were all treated as the same.

[3. Preparation of defective fermentation inhibitor]
[1. The mixed liquids in the stainless steel containers P1, Q1, R1 in which the screening of the microorganisms for the defective fermentation inhibitor was performed through the temperature lowering step by the screening of the microorganisms for the defective fermentation inhibitor] were separately housed in a cotton cloth bag, This cloth bag was subjected to a dehydrator to perform solid-liquid separation to obtain a microorganism-containing liquid (solid-liquid separation step).

  Next, about 9 kg of the solid-liquid separated microorganism-containing liquid was put into a stainless steel container P1, Q1, R1 in a fermentation aging can with hook (12 L capacity), and 0.1 ± 0.05 kg of maple syrup was added as a sugar source to homogeneity. The mixture was stirred for about 20 to 30 days at room temperature and atmospheric pressure for fermentation (first fermentation step).

  At the initial stage of the first fermentation step, a supernatant is formed on the surface of the liquid containing the microorganism, which, according to the experience of the inventors, was removed every several days in order to slow the fermentation. In addition, after this initial stage, it was confirmed that the microorganism-containing liquid started to bubble like a beer and fermentation was carried out by the microorganisms.

  Then, when the pH of the microorganism-containing liquid was 4.5 or less and the redox potential was -100 mV or less, the first fermentation step was terminated. At this point, no difference such as abnormal fermentation due to the difference in container shape between the stainless steel containers P1, Q1, and R1 was confirmed.

  Next, with respect to the microorganism-containing liquid that has undergone the first fermentation step, the aforementioned [2. Preparation of Stevia Stem Ripening Solution] was added and stirred uniformly, and allowed to stand for fermentation for about 20 to 25 days at room temperature and normal pressure (second fermentation step) ).

  Specifically, in a 10 L secondary fermentation can, 0.75 kg of the microorganism-containing liquid of one of the stainless steel containers P1, Q1 and R1 that has undergone the first fermentation step and 0.75 kg of Stevia stem ripening liquid are stored. Then, water was further added to make 10 kg, and the mixture was stirred uniformly to prepare a fermented liquid to be used in the second fermentation step. If necessary, a sugar source of about 0.75 ± 0.1 kg may be further added to the liquid to be fermented, and, for example, oligosaccharide, more preferably sugar beet sugar can be added.

  Then, the second fermentation step was terminated at the time of fermenting until the pH became 3.1 or less, and the obtained fermentation liquid was used as a poor fermentation inhibitor. Also at this time, no difference such as abnormal fermentation due to the container shape of the stainless steel containers P1, Q1, and R1 was observed, and in the subsequent experiments, the defective fermentation inhibitors were treated as the same.

[4. Biomass fermentation]
Next, biomass was fermented using the obtained poor fermentation inhibitor. Here, as the biomass, a mixed state in which chicken dung and livestock dung, dehydrated activated sludge, and food residues were mixed was used.

  Further, here, a test in which fermentation was performed without using a poor fermentation inhibitor (hereinafter referred to as a negative test) and a positive test in which fermentation was performed using a poor fermentation inhibitor were carried out. Positive tests are further described in [2. Preparation of Stevia Stem Ripening Solution], a poor fermentation inhibitor A prepared using the Stevia stem ripening solution and a poor fermentation inhibitor B prepared using the Stevia stalk simple ripening solution. Was examined.

  First, the average water content of 10000 kg of biomass was measured, and the water content required to make the water content 65-70% was calculated.

  Next, water was added to the poor fermentation inhibitor A or the bad fermentation inhibitor B to dilute the mixture until the required water content was obtained to prepare a diluted solution, which was sprayed with stirring so as to be uniformly mixed with the biomass ( Defective fermentation inhibitor mixing process). In addition, in the negative test, only the water having the above required water content was added to the biomass.

  The thus-prepared negative test biomass X, the biomass A1 mixed with the poor fermentation inhibitor A, and the biomass B1 mixed with the poor fermentation inhibitor B are separately deposited on a roofed deposition site to produce biomass. Was fermented (first biomass fermentation step).

  At this time, a strong malodor that seems to be a sulfur-containing odor component drifts from the biomass X, and a brown effluent having a strong odor is confirmed from the bottom of the deposited biomass X, and so-called poor fermentation is progressing. Was recognized. Further, the temperature rise due to the heat of fermentation was slow, and it took about 7 days until the temperature inside the surface layer of 40 to 50 cm reached 55 ° C.

  On the other hand, no strong offensive odor drifted from the biomass A1 and the biomass B1 and no ammonia odor derived from manure was felt. As for the odors of the biomass A1 and the biomass B1, a mild fermentation odor that was thought to be roughly lactic acid bacteria and yeast was felt. Further, the temperature rise due to the heat of fermentation was quicker than that of the biomass X. In particular, the biomass A1 was 2 days and the biomass B1 was 3 days, and it was confirmed that the biomass A1 is performing fermentation more smoothly than the biomass B1. No leaked liquid was confirmed.

  Next, when the internal temperature reached 55 ° C., the first biomass fermentation process was terminated for all the biomasses, and turning back was performed to supply air to the inside (second biomass fermentation process).

  At this time, with regard to the biomass X, a severe foul odor was generated by performing the turning back, and it seemed that the work in the vicinity of the village was not realistic. The temperature rise due to the heat of fermentation was slow, and it took about 30 days for the temperature to reach 75 ° C and 65 days for the water content to fall below 40%. During that time, the work of turning back was performed 15 times.

  On the other hand, the biomass A1 and the biomass B1 had a fruity odor, which was thought to be mainly due to yeast, when they were cut back, but did not have an odor such as a sulfur-containing odorous component that was generally perceived as a bad odor.

  Further, the temperature rise due to the heat of fermentation was relatively smooth, and in the case of the biomass A1, it took about 10 days until the temperature reached 75 ° C. and 35 days until the water content became 40% or less. During that time, the work of turning back was performed 7 times. Further, in the case of biomass B1, it took about 12 days until the temperature reached 75 ° C. and 45 days until the water content became 40% or less. During that time, the work of turning back was performed 10 times.

  Next, in any of the biomasses, when the water content once fell below 40%, water was added and uniformly mixed to bring the water content to 50%, and the state was again set to the deposition state. Hereinafter, this work is simply called watering work.

  Then, when the water content once fell below 40%, the fermentation heat of all the biomass was in a state of being settled at about 55 ° C, but the temperature increased again.

  This watering operation was repeated, and when the remarkable temperature rise was not observed and the water content fell below 40%, the second biomass fermentation step was terminated. The number of times the hydration work was required was twice for the biomass A1 and three times for the biomass B1, but five times for the biomass X, which means that the second biomass fermentation process did not end easily.

  Next, sieving was performed for each biomass that has undergone the second biomass fermentation step (sieving step).

  Then, the sieved biomasses were placed in a tertiary aging gauge in a piled state, and aging was performed until the water content was 20% or less (aging step).

  With respect to the biomass A1 and the biomass B1, the presence of white actinomycetes was generally confirmed on the surface in about 15 days after starting the aging step. On the other hand, with regard to the biomass X, although the presence of actinomycetes was partially confirmed, the global prevalence was not confirmed until the water content fell below 20%.

  Then, for any of the biomasses, the aging step is terminated when the water content falls below 20%, and the fermentation completion product A2 of the biomass A1, the fermentation completion product B2 of the biomass B1, and the fermentation completion product X2 of the biomass X are obtained. Got

  As described above, as a result of the above steps, according to the method for fermenting biomass according to the present embodiment, the odor generated from biomass can be reduced by fermentation, and furthermore, the fermentation of malodor-producing bacteria is suppressed to cause poor fermentation. It was shown that this can be prevented. In addition, compared with the conventional treatment with only actinomycetes, the fermentation could be carried out without being sensitive to the water content.

  Next, the state of the obtained fermented product and the fertilized state will be described. Fermentation completed product X2 had a peculiar unpleasant odor though it was in a sufficiently dry state, and when it was sprayed on the field, the field gave off an overall odor. In particular, it was judged to be unsuitable as a fertilizer because the unpleasant odor became stronger after it rained. At the same time, the fertilizing effect on the crops was not seen so much.

  On the other hand, the fermented product A2 and the fermented product B2 had almost no odor in a sufficiently dried state, and had a very weak earthy odor or lactic acid bacterium odor.

  Further, with regard to the fermented products A2 and B2, no unpleasant odor was felt even when the fertilizer was applied to the field, and the same was observed after rainfall. Furthermore, a sufficient fertilization effect was observed for the crops, and it was shown that the fertilizer can be used as a good fertilizer as compared with the case of non-fertilization and the fermentation-completed product X2.

  As described above, according to the method for screening a microorganism for poor fermentation inhibitor according to the present embodiment, 0.6 ± 0.1 parts by weight of dry stevia stem powder, 0.6 ± 0.1 parts by weight of rice bran powder, and 0.6 ± 0.1 parts by weight. Part of dried okara powder and 12 ± 1 part by weight of brackish water containing photosynthetic bacteria, a standing step of standing a mixed solution for a predetermined time, and the mixed solution that has passed through the standing step into a predetermined container The container containing step of forming a weak sterilization area of 20 cm or more from any outer surface of the containing shape of the same mixed solution in the contained mixed solution, and 25 cm or less, and the container containing step. After that, a predetermined container containing the mixed liquid is placed in the heating space, and the heating space is heated from a state of normal temperature and normal pressure to a state of 150 to 160 ° C. at about 2.5 atmospheric pressure for 45 to 60 minutes, and then heated. Maintain the condition of 150-160 ℃ at 2.5 atm for 1-3 minutes, then heat the heating space at approximately 2 atm for 115-12 Set the temperature down to 3 ℃ to 5 ℃ and maintain the temperature at 115 ℃ to 125 ℃ for 20 to 40 minutes at about 2 atm, and then heat the heating space to normal temperature and normal pressure for 24 to 30 hours. By lowering the temperature, a microorganism selection step of leaving the microorganisms that have been heat-selected in the mixed solution, and the odor generated from the biomass can be reduced by fermentation without being relatively trapped in the water content, Moreover, it is possible to provide a method for screening a microorganism for a defective fermentation inhibitor, which can prevent the fermentation of malodor producing bacteria and prevent defective fermentation.

  Finally, the above description of each embodiment is an example of the present invention, and the present invention is not limited to the above embodiment. Therefore, it goes without saying that various modifications other than the above-described embodiments can be made according to the design and the like as long as they do not deviate from the technical idea of the present invention.

Claims (4)

Mix 0.6 ± 0.1 parts by weight of dry Stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, 0.6 ± 0.1 parts by weight of dry okara powder, and 12 ± 1 parts by weight of brackish water containing photosynthetic bacteria A standing step of standing the mixed solution for a predetermined time,
The mixed solution that has undergone the stationary step is housed in a predetermined container, and is 10 cm or more from any position on the outer surface of the housed shape of the mixed solution in the housed mixed solution, and at least on the outer surface. A container accommodating step that forms a weak sterilization area that is 17 cm or less from any position,
A predetermined container containing the mixed solution through the container storing step is arranged in a heating space, and the heating space is heated from a state of normal temperature and normal pressure to a state of about 2.5 atm to a temperature of 150 to 160 ° C for 45 to 60 minutes. The temperature is raised and maintained at 150 to 160 ° C at about 2.5 atm for 1 to 3 minutes, and then the heating space is set to 115 to 125 ° C at about 2 atm for 3 to 5 minutes to set the temperature down to about 2 Maintain the temperature of 115-125 ° C for 20-40 minutes at atmospheric pressure, further lower the temperature of the heating space to the state of normal temperature and normal pressure over 24-30 hours, and leave the microorganisms selected by heating in the mixed solution. Microbial selection process,
A method for screening a microorganism for a poor fermentation inhibitor having: Mix 0.6 ± 0.1 parts by weight of dry Stevia stalk powder, 0.6 ± 0.1 parts by weight of rice bran powder, 0.6 ± 0.1 parts by weight of dry okara powder, and 12 ± 1 parts by weight of brackish water containing photosynthetic bacteria A standing step of standing the mixed solution for a predetermined time,
The mixed solution that has undergone the stationary step is housed in a predetermined container, and is 10 cm or more from any position on the outer surface of the housed shape of the mixed solution in the housed mixed solution, and at least on the outer surface. A container accommodating step that forms a weak sterilization area that is 17 cm or less from any position,
A predetermined container containing the mixed solution through the container storing step is arranged in a heating space, and the heating space is heated from a state of normal temperature and normal pressure to a state of about 2.5 atm to a temperature of 150 to 160 ° C for 45 to 60 minutes. The temperature is raised and maintained at 150 to 160 ° C at about 2.5 atm for 1 to 3 minutes, and then the heating space is set to 115 to 125 ° C at about 2 atm for 3 to 5 minutes to set the temperature down to about 2 Maintain the temperature of 115-125 ° C for 20-40 minutes at atmospheric pressure, further lower the temperature of the heating space to the state of normal temperature and normal pressure over 24-30 hours, and leave the microorganisms selected by heating in the mixed solution. Microbial selection process,
At least a solid-liquid separation step for obtaining a microorganism-containing liquid by solid-liquid separating the mixed solution that has undergone the microorganism selection step by means capable of transferring to a liquid phase.
A first fermentation step in which a sugar source is added to the obtained microorganism-containing liquid and fermentation is performed at room temperature and normal pressure for a predetermined time, the pH of the microorganism-containing liquid is 4.5 or less, and the redox potential is -100 mV or less,
A second fermentation step of adding a ripening solution of Stevia stalk to the microorganism-containing solution that has undergone the first fermentation step and fermenting it until the pH becomes 3.1 or less to obtain a poor fermentation inhibitor. A method for producing a defective fermentation inhibitor. The stevia stem ripening solution is
Stevia stalk dispersion preparation step of adding 10 ± 2 parts by weight of water to 1 part by weight of dry stevia stalk powder to prepare a dispersion of stevia stalk,
The Stevia stalk dispersion obtained through the Stevia stalk dispersion preparation step is stored in a predetermined container, and from any position on the outer surface of the stored shape of the Stevia stalk dispersion in the stored Stevia stalk dispersion. Stevia stalk dispersion container storage step of forming a weak sterilization area that is 10 cm or more, and is 17 cm or less from at least any position on the outer surface,
A predetermined container containing the Stevia stem dispersion liquid is placed in the heating space through the Stevia stem dispersion liquid container storing step, and the heating space is maintained at room temperature and normal pressure from about 2.5 atm to 150 to 160 ° C. It takes 30 to 40 minutes to raise the temperature, and the state of 150 to 160 ° C is maintained at about 2.5 atm for 1 to 3 minutes, and then the heating space is kept at about 2 atm to 115 to 125 ° C for 3 to 5 minutes. By setting the temperature to fall, maintaining the condition of 115 to 125 ° C. at about 2 atm for 20 to 40 minutes, and further cooling to 85 to 95 ° C., the dry stevia stalk derived from the heat selected in the stevia stalk dispersion is heated. Stevia stem-derived microorganism selection step to leave microorganisms,
Stevia stalk dispersion liquid cooled to 85-95 ℃ was solid-liquid separated at least by means capable of transferring microorganisms to the liquid phase, and the liquid phase Brix value was 2.0-4.0 and Stevia stalk-derived microorganisms were contained. Stevia stalk extract preparation step for obtaining stevia stalk extract,
The Stevia stalk extract is heated and boiled down and then cooled, Brix value is 4.0 to 7.0 and pH is 6.0 or less, ORP is 10 to 99 mV Stevia stalk concentrate to obtain an extract concentration step,
The stevia stem concentrated liquid is aged at room temperature to obtain a Stevia stem ripening liquid having a pH of 5.0 or less and an ORP of -100 mV or less. A method for producing a fermentation inhibitor. While diluting 1 part by weight of the poor fermentation inhibitor obtained by the method for producing a poor fermentation inhibitor according to claim 2 or 3 with a predetermined amount of water to prepare a diluent, A poor fermentation inhibitor mixing step of adding water content to 10000 parts by weight of biomass to a water content of 65 to 70%;
A first biomass fermentation step in which the biomass that has passed through the poor fermentation inhibitor mixing step is allowed to stand in a piled state until the temperature of the inside of the surface layer reaches 40 to 50 cm reaches 55 ° C;
A second biomass fermentation step in which the biomass that has undergone the first biomass fermentation step is cut back and aerobically fermented until the moisture content of the biomass falls below 40% while maintaining a temperature not exceeding 75 ° C.
A sieving process of sieving the biomass that has undergone the second biomass fermentation process while unraveling it;
A method for fermenting biomass, comprising a aging step in which the biomass that has been subjected to a sieving step is put into a piled state and allowed to stand until the water content becomes 20% or less.