JP6714239B2 - Cosmetics - Google Patents

Description

The present invention relates to cosmetics, and more particularly to a skin cosmetic that physiologically healthens the skin and scalp by an immunostimulating action, a moisturizing action, etc. and improves the condition of the skin and hair.

A cosmetic that contains porphyran, which is one of the sulfated polysaccharides obtained from seaweeds of the genus Porphyra, improves the condition of skin and hair by physiologically sounding the skin, scalp and hair through immunostimulating action and moisturizing action. It is known (see, for example, Patent Document 1).

Further, porphyran alone is used by a skin external preparation containing 0.1 to 30% by mass of a seaweed extract containing porphyran as a ceramide production promoter and 0.001 to 5% by mass of intercellular lipid and/or its related substance. It is known that a superior moisturizing effect and a rough skin improving effect are obtained, and these effects can be obtained with a relatively small amount of porphyran added, as compared with the case (see, for example, Patent Document 2).

Further, by using porphyran as the main material of the cosmetic pack, the water solubility is high, the transparency is excellent, the adhesion to the skin is high, and the transparency is excellent when it is spread on a part of the skin. A makeup pack that does not need to be peeled off to form an ultrathin film with less discomfort on the skin and to be washed off has also been proposed (for example, see Patent Document 3).

JP, 2005-336148, A JP, 2013-133324, A JP, 2008-255066, A

However, the cosmetics containing porphyran have problems such as stickiness when applied to the skin and a feeling of foreign matter such as fiber. Therefore, when diluted with water or an oil agent to such an extent that it does not give a sticky feeling or a foreign-body sensation, the desired moisturizing effect and immunostimulating effect cannot be obtained.

Therefore, it is an object of the present invention to provide a cosmetic composition which retains the high immunostimulatory action and moisturizing action of porphyran and at the same time reduces stickiness and foreign body sensation when applied to the skin, and a method for producing the same.

In solving the above problems, the present inventors have found that porphyran having a relatively low molecular weight is a factor of stickiness and foreign body sensation when applied to the skin.

That is, by adding a relatively high molecular weight porphyran, the desired moisturizing action and immunostimulatory action can be obtained with a small addition amount, so that the stickiness and foreign body sensation when applied to the skin as a cosmetic can be reduced. It is a thing.

Further, in order to obtain a porphyran having a relatively high molecular weight, the present inventors heated 100° C. to 150° C. and applied 0.1 MPa to 1.0 MPa at the time of extracting the raw material seaweed by hot water treatment. It was found that by applying pressure, decomposition of relatively high molecular weight porphyran can be minimized, and the extraction operation can be simplified and shortened.

The cosmetic according to the present invention is a cosmetic containing porphyran, wherein the porphyran has a maximum peak of the molecular weight distribution in the range of molecular weight of 1,000,000 or more and 6,000,000 or less.

According to the present invention, it is possible to provide a cosmetic composition that retains the high immunostimulatory action and moisturizing action of porphyran and at the same time reduces stickiness and foreign body sensation when applied to the skin.

FIG. 1a is a micrograph of fibroblasts cultured with porphyran as a medium, and FIG. 1b is a micrograph of fibroblasts cultured with a commercially available culture dish as a medium. 2a is a micrograph of mouse skin cells cultured in agarose medium, FIG. 2b is a micrograph of mouse skin cells cultured in carrageenan, and FIG. 2c is a microscope image of mouse skin cells cultured in porphyran. Fig. 2d is a photomicrograph of mouse skin cells cultured in a commercial culture dish as a medium. FIG. 3 is an example of a molecular weight distribution curve obtained by measuring porphyran used in the cosmetic of the present invention using GPC.

Porphyran is a viscous polysaccharide containing galactose and a galactose derivative contained in seaweed, which is a seaweed belonging to the genus Porphyra, as a constituent component. Since it has a sulfate group, it is water-soluble even if it is a polymer. Porphyran is extracted by subjecting seaweed to hot water treatment. The extract is treated with activated carbon and diatomaceous earth, decolorized, deodorized, concentrated, and then powdered by precipitation powdering by adding alcohols, spray drying, drying with a drum dryer, freeze drying, and the like. In addition, the concentrated solution is desalted through an ion exchange resin or ion exchange membrane, and then neutralized with alkali such as caustic soda, caustic potash, magnesium hydroxide, calcium hydroxide, or basic amino acids such as arginine, ornithine, and histidine. It can also be prepared as a single salt and modified porphyran, and the details thereof are described in JP-A-2005-255932 and JP-A-2008-255066.

Porphyran according to the prior art, which has been extracted by hot water treatment and then alkali-treated, has an average molecular weight of about 150,000 to 200,000, and the maximum peak of the molecular weight distribution is also about 150,000 to 200,000. On the other hand, the porphyran according to the present invention has the maximum peak of the molecular weight distribution in the range of 1,000,000 or more and 6,000,000 or less, and the average molecular weight is 1,000,000 or more. That is, when the maximum peak of the molecular weight distribution is less than 1,000,000, the moisturizing effect and cell activation are decreased, and the content of porphyran must be increased, and as a result, stickiness during application and a feeling of foreign matter of fibrous material are caused. Occurs.

In order to adjust such a high molecular weight porphyran, 100° C. to 150° C. and 0.1 to 1.0 MPa of pressure are applied to the seaweed, which is a raw material, during extraction by hot water treatment. Under such conditions, it is possible to extract high molecular weight porphyran, and at the same time, it is possible to minimize the decomposition of the extracted high molecular weight porphyran, which simplifies and shortens the extraction operation. You can

Preferably, the hot water treatment is performed under alkaline conditions with an ammonia concentration of 10 ⁻⁶ to 10 ⁻⁵ M and a pH of 8 to 9.

Here, the molecular weight distribution of the porphyran used in the cosmetic of the present invention is measured using gel permeation chromatography (GPC) under the following conditions, for example.
Measuring device: CCPD manufactured by Tosoh Corporation
Column: TSKgel G5000PW [manufactured by Tosoh Corporation] 12 pieces Measurement temperature: 30°C
Sample solution: 0.1 mm phosphate buffer solution solution injection amount: 100 μl
Detection device: UV-visible spectrophotometer UV8000 (detection wavelength 210 nm) and refractive index detector RI8000
Reference material: Pharmacia dextran

More preferably, porphyran containing 50% by mass or more of a component having a molecular weight of 1,000,000 or more is used in the cosmetic of the present invention. When the content of the component having a molecular weight of 1,000,000 or more is 50% by mass or more, a high moisturizing effect and cell activation are secured. The ratio of components having a molecular weight of 1,000,000 or more is calculated from the area of the molecular weight distribution curve obtained by the above GPC.

FIG. 3 is an example of a molecular weight distribution curve obtained by measuring the porphyran used in the cosmetic of the present invention using the GPC. In the figure, the peak of the molecular weight distribution is 2,000,000, the ratio of the molecular weight of 1,000,000 or more is 51%, and the ratio of the molecular weight of 1,000,000 to 6,000,000 is 32%.

The cosmetic of the present invention contains porphyran as an essential component, and the content thereof is preferably a lower limit of 0.001 mass% or more as a solid content. If the concentration is lower than that, the effect of the present invention may not be sufficiently exhibited. In addition, the upper limit is 0.5% by mass or less as a tactile evaluation in order to prevent stickiness at the time of coating and the feeling of foreign matter of fibrous substance from occurring because porphyran has viscosity.

In the production of a cosmetic, the porphyran according to the present invention is added to the cosmetic in a state of being previously dissolved in a solvent containing water as a main component (hereinafter, also referred to as a cosmetic undiluted solution) to obtain a desired solid content. It is preferable to adjust the content. The concentration of the cosmetic undiluted solution is not particularly limited as long as it is the content of porphyran capable of exerting the effect of the present invention in the cosmetic to which the cosmetic undiluted solution is added, but the molecular weights of the porphyrans according to the present invention are comparative. Since it tends to have a higher viscosity than an aqueous solution in which the same amount of low molecular weight porphyran is dissolved, the concentration (for example, 0.01% by mass or more and 5% by mass or less) in consideration of handleability in preparation and addition of a cosmetic concentrate. ) Is preferable. Preferably, it is 0.1% by mass or more and 0.8% by mass or less.

Further, the cosmetics of the present invention include various components used in ordinary cosmetics, quasi drugs, pharmaceuticals, etc., for example, oil agents, powders, surfactants other than nonionic surfactants, viscous agents, resins. , Amino acids, saccharides, organic UV absorbers, natural plant extract components, solvents, preservatives, sequestering agents, anti-inflammatory components, medicinal components and the like can be appropriately added. These other components may be used alone or in combination of two or more. In addition, the content of the other components in the cosmetic can be appropriately selected within a range that does not impair the effects of the present invention.

Examples of the oil agent include volatile and non-volatile oil agents, solvents, resins and the like which are usually used for external preparations for skin, and may be liquid, paste or solid at room temperature. Examples of the oil agent include behenyl alcohol, higher alcohols such as octyldodecanol, lauric acid, myristic acid, stearic acid, palmitic acid, fatty acids such as isostearic acid and undecylenic acid, glycerin, diglycerin, sorbitol, ethylene glycol, dipropylene glycol. Polyhydric alcohols such as propylene glycol, polyethylene glycol, pentanediol and isopropylene glycol, glyceryl monostearate, glyceryl trioctanoate, glyceryl tri-2-heptylundecanoate, diethyl phthalate, ethylene glycol monostearate, oxystearic acid Esters such as octyl and diisostearyl malate, hydrocarbons such as liquid paraffin, paraffin, petrolatum and squalane, mink oil, cacao butter, coconut oil, palm oil, camellia oil, sesame oil, macadamia nut oil, meadow home oil, jojoba Oils and fats such as castor oil and olive oil, and vegetable oils can be mentioned.

Examples of powders are Red No. 104, Red No. 102, Red No. 226, Red No. 201, Red No. 202, Yellow No. 4, Blue No. 1, Black No. 401, Yellow No. 4 Aluminum Lake, Yellow No. 5 Aluminum lake, lake pigment such as yellow No. 203 barium lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, cellulose powder, zinc oxide, titanium oxide, white pigment such as cerium oxide, talc, Examples thereof include inorganic powders such as mica, sericite, kaolin, silica, and alumina, bentonite, smectite, boron nitride, fine particles of titanium oxide, fine particles of zinc oxide, and the like, which are conventionally known surface treatments such as N-acylation. It is also possible to use those that have been subjected to lysine treatment, amino acid treatment, hydrophilic polymer treatment, oil treatment, silicone treatment, metal soap treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment and the like. There is no particular limitation on the powder shape (spherical shape, rod shape, needle shape, plate shape, irregular shape, scaly shape, spindle shape, etc.). The size of the powder is preferably in the range of 5 nm to 100 μm, more preferably 10 nm to 25 μm. These powders may be treated alone or formed into a mixture and treated together. Further, it is also possible to process the mixture in which the color of the mixture is adjusted to a skin color or the like. Further, it is possible to obtain a treated powder having an ultraviolet protection function by using an ultraviolet scattering component such as fine particle titanium oxide or fine particle zinc oxide.

As the surfactant other than the nonionic surfactant, for example, an anionic surfactant, a cationic surfactant, and an amphoteric surfactant can be used. More specifically, fatty acid soap, α-acyl sulfonate, N-acyl amino acid, N-acyl taurine salt, N-acyl glutamate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate. Anionic surfactants such as salts, alkyl ether sulfates, alkyl amide sulfates, alkyl phosphates, alkyl amide phosphates, alkyloyl alkyl taurine salts, sulfosuccinates, perfluoroalkyl phosphates, alkyl trimethyl chloride Cationic surfactants such as ammonium, auteallyl trimethyl ammonium ammonium chloride, stearyl trimethyl ammonium bromide, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium chloride, behenyl trimethyl ammonium bromide, carboxybetaine type, sulfo Amphoteric surfactants such as betaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylic acid salt type, imidazoline derivative type and amidoamine type are mentioned.

Examples of the sticky agent and resin include sodium polyacrylate, cellulose ether, alginate, carboxyvinyl polymer, pullulan, deoxyribonucleic acid and its salts, acidic mucopolysaccharides such as chondroitin sulfate and its salts, tamarind seed polysaccharides, tuberose polysaccharides. , Agar, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, locust bean gum, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, hyaluronic acid and its salts, albumin, casein, curdlan, Examples thereof include gellan gum, dextrin, cyclodextrin, dextran, cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, hydroxypropyl methyl cellulose and the like.

As the amino acids, L-glutamic acid, L-leucine, L-serine, sodium L-aspartate, L-threonine, DL-alanine, L-isoleucine, L-phenylalanine, L-arginine, L-proline, L-tyrosine. , L-histidine hydrochloride, lysine hydrochloride, glycine, N-acetyl-L-glutamic acid, sodium PCA, trimethylglycine, γ-polyglutamic acid and the like.

Examples of the saccharide include galactose, mannose, maltose, fructose, sucrose, xylose, sorbitol, xitol, mannitol and the like.

Examples of organic UV absorbers include 2-ethylhexyl paramethoxycinnamate, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfuric acid, 2,2'-dihydroxy-4-. Methoxybenzophenone, p-methoxyhydrocinnamic acid diethanolamine salt, paraaminobenzoic acid (hereinafter abbreviated as PABA), octyldimethyl PABA, octyl salicylate, 2-phenyl-benzimidazole-5-sulfate, triethanolamine salicylate, 3-( 4-methylbenzylidene)camphor, 2,4-dihydroxybenzophenine, 2,2',4,4'-tetrahydroxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2-hydroxy-4 Examples include -N-octoxybenzophenone, 4-isopropyldibenzoylmethane, butylmethoxydibenzoylmethane, 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl and the like. To be

Examples of natural plant extract components include acitaba extract, avocado extract, armature extract, altea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo biloba extract, fennel extract, turmeric extract, oolong tea extract, and ages extract. , Echinacea leaf extract, sardine extract, psyllium extract, scutellaria extract, barley extract, St. John's wort extract, sardine extract, Dutch mustard extract, olive leaf extract, orange extract, seawater dried product, seaweed extract (other than porphyran-containing seaweed extract) , Hydrolyzed Elastin, Hydrolyzed Wheat Powder, Hydrolyzed Silk, Chamomile Extract, Carrot Extract, Kawara Mugwort Extract, Licorice Extract, Karkade Extract, Kakoku Extract, Kiwi Extract, Quina Extract, Cucumber Extract, Guanosine, Gardenia Extract, Kumazasa Extract, Clara Extract , Walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, yeast extract, burdock extract, rice bran extract, rice germ oil, comfrey extract, collagen, cowberry extract, cinnamon extract, psycho extract, Saitai Extract, Salvia Extract, Sorbour Extract, Sasa Extract, Hawthorn Extract, Hawthorn Extract, Shiitake Extract, Ziou Extract, Shikon Extract, Perilla Extract, Cinnamon Extract, Spiraea Root Extract, Peonies Extract, Ginseng Root Extract, Birch Extract, Horsetail Extract, Poison Ivy Extract, hawthorn extract, Sambucus nigra extract, yarrow extract, mint extract, sage extract, mallow extract, ginseng extract, assembly extract, soybean extract, taiso extract, thyme extract, tea extract, clove extract, chigaya extract, chimpanzee extract, touki extract , Scutellaria barbata extract, Tounin extract, Spruce extract, Dokudami extract, Tomato extract, Natto extract, Carrot extract, Garlic extract, Novara extract, Hibiscus extract, Bakumondou extract, Lotus extract, Parsley extract, Honey, Hamamelis extract, Parietaria extract, Hikiokoshi Extract, bisabolol, loquat extract, coltsfoot extract, fukinoto extract, butterbur extract, butcher bloom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, bodai Juju extract, button extract, hop extract, pine extract, horse chestnut extract, mizubasha extract, sucrose extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, yukinoshita extract, yuzu extract, yokuinin extract, mugwort extract, lavender extract, apple extract, lettuce extract, Examples thereof include lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract and the like.

Examples of the solvent include purified water, ethanol, lower alcohols, ethers, LPG, fluoroalcohol, volatile linear silicone, and next-generation CFCs.

Examples of preservatives include benzoic acid, sodium benzoate, undecylenic acid, salicylic acid, tehydroacetic acid or salts thereof, methyl paraoxybenzoate, ethyl paraoxybenzoate, isopropyl paraoxybenzoate, organic acids such as benzyl paraoxybenzoate and the like. Derivatives, isopropylmethylphenol, chlorhexidine gluconate, thymol, parachlorophenol, phenylethyl alcohol, phenylphenol, sodium phenylphenol, phenoxyethanol, phenoxydiglycol, phenols, phenols such as benzyl alcohol, stearyltrimethylenemonium chloride, cetyl chloride Trimethylammonium, cetylpyridinium chloride, benzalkonium chloride, benzethonium chloride, methylbenzethonium chloride, laurylmethylammonium chloride, alkylisoquinolium bromide, quaternary ammonium salts such as domiphen bromide, tea extract, hinokitiol, apple extract, rose Examples include plant extracts such as marie extract, chloramine T, chlorhexidine, zinc pyrithione and the like. Of these, 1,3-butylene glycol, hevenyl alcohol, rose water, rosemary extract, and tea extract are preferable.

Examples of the sequestering agent include ethylenediaminetetraacetic acid disodium, edetic acid, edetic acid disodium and etidronic acid.

Examples of the anti-inflammatory component include licorice leaf extract, hamamelis water, dipotassium glycyrrhizinate, allantoin, tocopherol acetate, tocopherol, and azulene. Of these, licorice leaf extract and hamamelis water are preferred.

Examples of the medicinal component include vitamin A oil, retinol, riboflavin, pyridoxine hydrochloride, L-ascorbic acid, magnesium L-ascorbyl phosphate, L-ascorbic acid monopalmitate, L-ascorbic dipalmitate, L-. Examples thereof include ascorbic acid-2-glucoside, pantothenate, vitamin D2, cholecalciferol and the like.

The cosmetic of the present invention can be manufactured into any dosage form such as a solubilizing system, an emulsifying system, a powder dispersion solubilizing system, a powder dispersion emulsifying system, a powder dispersion oil system and the like according to a conventional method. The cosmetic of the present invention is preferably adjusted to pH 2 to 11, particularly pH 3 to 9. The cosmetics of the present invention include skin care products such as lotion, emulsion, cream and cosmetic oil, makeup cosmetics such as foundation, powder, lipstick, blusher, eye shadow, nail enamel, tablets, capsules and granules. It can be suitably used as a product such as a bathing agent in the form of powder, powder, liquid or the like.

Hereinafter, the present invention will be described more specifically by way of examples, but the present invention is not limited to these exemplifications.

(Adjustment of Porphyran)
(1) 1 kg of air-dried laver from Ariake, Kyushu was suspended in 10 kg of boiling deionized water containing 6×10 ⁻⁶ M ammonia, and autoclaved (110° C., 30 minutes). Next, the residue was completely removed by suction filtration using kitchen paper and centrifugation (8000 rpm, 40 minutes), the filtrate was removed of ammonia by an aspirator, and further concentrated under reduced pressure (1 mmHg/30° C.). In the case of protein removal, an operation of passing through a cation exchange resin column was performed. Then, the filtrate was concentrated with an evaporator and dialyzed with a dialysis tube for 5 days.

(2) The porphyran solution obtained in step (1) was concentrated with an evaporator and freeze-dried to obtain a white purified product (porphyran). The yield of the purified product was 14.3% in terms of weight. When the molecular weight distribution of the porphyran obtained in this step was measured by HPLC, it was confirmed that it had a maximum peak at a molecular weight of 4,880,000.
Further, by changing the concentration of ammonia used in the autoclave treatment in the step (1), four types of porphyrans having maximum peaks at molecular weights of 1.2 million, 2 million and 6 million were adjusted.

(Adjustment of comparative Porphyran)
(3) The above steps (1) except that 1 kg of dried Ariake seaweed from Kyushu, Kyushu was suspended in 10 kg of boiling deionized water containing 0.1 M ammonia and autoclaved (100° C., 30 minutes). ) And the same treatment as the step (2), to prepare a porphyran having a maximum peak at a molecular weight of 100,000.
(4) A 1.5% porphyran solution obtained by dissolving the porphyran obtained in step (2) with deionized water was passed through a Diaion PK216 (0.5 L) column. Then, the solution was passed through a DIAION PA412 (1 L) column, flowed at a flow rate of 3.5 L/hr, then 3 L of deionized water was passed through, and the passed solution was collected until the solid content concentration became zero. The column solution was neutralized with 30% NaOH or 10% free arginine solution to pH 6.5 to obtain a porphyran Na salt solution and a porphyran arginine salt solution. Diatomaceous earth was added to this solution and filtered to obtain a porphyran Na salt solution and a porphyran arginine salt solution.

(5) The Na salt solution of porphyran obtained in step (4) was concentrated to obtain a 3% porphyran aqueous solution. This solution was adjusted to pH 4.0 with sulfuric acid, allowed to stand at 55° C. for 0 to 70 minutes, and then neutralized to pH 6.5 again with caustic soda to reduce the molecular weight of the porphyran low-molecular solution (the maximum peak of the molecular weight distribution was about 20). 10,000). Similarly, a low molecular weight porphyran solution (maximum peak of molecular weight distribution: about 400,000) was obtained from the porphyran arginine salt solution obtained in step (4).

(Confirmation of cell activation)
Fibroblasts (NIH3T3) were spread on a medium at a seeding density of 80,000 cells/cm ² and cultured in an incubator (37°C, 0.5% CO ₂ atmosphere, relative humidity 100%) for 72 hours, followed by fluorescent staining. (Dimethylaminophenyl, rhodamine dye) was performed, and the cells on the surface were observed with a microscope. As a medium for fibroblasts, a 0.03% by mass aqueous solution of porphyran (molecular weight peak of 4,880,000) obtained in step (2) was immobilized on a glass substrate with tetramethylene diglycidyl ether (TGDE) to neutralize it. A sample that had been washed with water until that time was used. Compared with the commercially available culture dish (Fig. 1b), the sample using the porphyran obtained in the step (2) as a medium had comparable fibroblast density (Fig. 1a).

After developing mouse skin cells in a medium and culturing in an incubator (37°C, 0.5% CO ₂ atmosphere, relative humidity 100%) for 72 hours, DAPI (4',6-diamidino) that stains cell nuclei with fluorescence (2-phenylindole) and rhodamine that stains the actin component of cells, and the cells on the surface were observed with a microscope. As a medium for mouse skin cells, 0.03% by mass aqueous solution of porphyran (molecular weight peak of 4.88 million), carrageenan, and agarose obtained in step (2) was added onto a glass substrate with tetramethylene diglycidyl ether (TGDE). Samples that were fixed and washed with water until neutral were used. Almost no growth of skin culture was observed on the agarose medium (Fig. 2a). On the other hand, proliferation of skin cells was observed in carrageenan (Fig. 2b) and porphyran (Fig. 2c), and especially in porphyran, cell nuclei per unit area (white point in the figure) at the same level as in commercially available culture dishes (Fig. 2d). It was found that the actin site (gray part in the figure) was also proliferating at high density.

(Panel test using beauty essence)
For 10 women (25 to 50 years old) who usually complain of rough skin and dryness for some reason, the cosmetics were actually used, and a sticky feeling at the time of application, a feeling of foreign matter at the time of application, a moisturizing effect (moisturizing effect) Effect) and rough skin improving effect (cell activation) were examined. While observing the condition of the skin every day, the test subject was exfoliated by washing the face once a day, and then the beauty essence shown in Tables 1 and 2 (Examples 1 to 6 and Comparative Examples 7 to 11) prepared by the following prescription examples. ) Was applied in an appropriate amount. The application was continued for one month, and the skin conditions at the start of the test and at the end of the test were compared to evaluate the following items.

The evaluation items are "no stickiness during application," "no fibrous material remains after application," "moisturized dry skin," "texture became fine," "smooth skin," "moisture retention." 5 items. The test results are shown in the table based on how many of the 10 respondents answered that each item was improved. The humidity retention is measured by using a commercially available moisture retention checker at the start of the test and at the end of the test, and is shown by the number of people who have improved the moisture retention at the end of the test by 5% or more as compared with the start of the test.

From the results of Tables 1 and 2, in Comparative Examples 8 to 11 in which the maximum peak of the molecular weight distribution was 200,000 or 400,000, the moisturizing action and the cell activating activity were compared with Comparative Example 7 containing no porphyran. In order to exert the effect of, the content has to be increased, and stickiness or a feeling of foreign matter of fibrous material is generated, and with a content in which the stickiness or the feeling of foreign matter is reduced, sufficient moisturizing effect and cell activation are obtained. The sexual effect was not obtained.

On the other hand, in Examples 1 to 6 in which the maximum peak of the molecular weight distribution is porphyran having a molecular weight of 1,000,000 or more and 6,000,000 or less, even a small content has a sufficient moisturizing action and cell activating activity, and has a feeling of use. Was also good. In particular, in Examples 5 and 6 containing porphyrans in which the proportion of components having a molecular weight of 1,000,000 or more was 90% by mass, sufficient moisturizing action and cell activating activity were obtained even if the content of porphyrans was further reduced. It was confirmed that the feeling was further improved.

In the preparation of the beauty essences of Examples 1 to 6, instead of the 3% by mass solution of porphyran used as the cosmetic stock solution, a 0.20% by mass solution of porphyran identical to the porphyran used in Examples 1 to 6 was applied. When the usability was evaluated with the beauty essences of Examples 21 to 26 which were used as stock solutions and the addition amount was 3% by mass, the same results as those of Examples 1 to 6 were obtained.

(Manufacture of various formulations)
Various cosmetics according to the present invention were manufactured. The formulation examples are shown below, but the present invention is not limited thereto.

Example of manufacturing lotion. A lotion was manufactured according to the following formulation.
mass%
1. Porphyran 3% solution 10.0
2.1.2-Pentanediol 1.0
3.1,3-butylene glycol 1.5
4. Scutellaria extract 5.0
5. Soybean fermented extract 2.0
6. Royal Jelly Extract 5.0
7. Residual amount of purified water 100

Cream production example. A cream was produced according to the following formulation.
mass%
1. Porphyran 3% solution 10.0
2. Rose water 3.0
3. Stearic acid 8.0
4. Hamamelis water 2.0
5. Allantoin 0.5
6. Sorbit liquid 3.5
7. Urea 3.0
8.1,3-butylene glycol 1.5
9. Coconut oil fatty acid 2.0
10. Glyceryl stearate 2.0
11. Behenyl alcohol 5.0
12. Natural vitamin E 0.05
13. Residual amount of purified water 100

Shampoo manufacturing example. A shampoo was manufactured according to the following formulation.
mass%
1. Porphyran 3% solution 2.5
2. Coconut oil fatty acid amide propyl betaine 8.0
3. Coconut oil fatty acid dietanomile 9.5
4. O-hydroxyethyl cellulose chloride 5.0
5. Jojoba oil 2.0
6. Paraoxin benzoyl estil 2.2
7. Perfume 0.1
8. Residual amount of purified water 100

Claims (3)

A cosmetic containing porphyran,
The said porphyran has a maximum peak of molecular weight distribution in the range of molecular weight 1 million or more and 6 million or less, Cosmetics characterized by the above-mentioned. The cosmetic according to claim 1, wherein the porphyran contains 50% by mass or more of components having a molecular weight of 1,000,000 or more. Content of the said porphyran is 0.001 mass% or more and 0.5 mass% or less, The cosmetics of Claim 1 or 2 characterized by the above-mentioned.