JP6748735B2 - Microorganisms capable of controlling plant diseases - Google Patents

Description

TECHNICAL FIELD The present invention relates to a microorganism having an ability to control plant diseases. The present application claims priority to Japanese Patent Application No. 2016-232222 filed on Nov. 30, 2016, the content of which is incorporated herein by reference.

A large number of chemically-synthesized pesticides are used as the main control method for plant diseases. However, chemically-synthesized pesticides have few kinds of action points, and resistance to plant pathogens has become a problem in recent years due to repeated administration. In particular, for bacterial plant diseases, inorganic and organic copper agents, a few kinds of antibiotics such as kasugamycin, streptomycin, oxytetracycline, and as a synthetic antibacterial agent, only one kind of oxolinic acid is registered, It is used. The development of resistant bacteria is serious for antibiotics and synthetic antibacterial agents, and their use may be restricted. Further, the inorganic and organic copper agents are liable to cause phytotoxicity on agricultural and horticultural crops, and there are restrictions on the time of use and crops used.

Therefore, in order to overcome the problems of resistant bacteria and phytotoxicity, interest in biological pesticides is increasing as a means of replacing or using together with conventional synthetic fungicides. Biological pesticides have advantages over conventional synthetic bactericides such as less environmental pollution, harmony with the ecosystem and superior control effect.
As a microbial pesticide used for controlling bacterial diseases of agricultural and horticultural crops, for example, Pseudomonas rhodesiae or a mutant thereof having an ability to control plant diseases is known (Patent Document 1). Further, a fungicide composition for agricultural and horticultural use containing Pseudomonas sp. CAB-02, which is particularly used for controlling rice seedling bacterial disease and seedling bacterial killing disease, which are seed-borne bacterial diseases of rice. Has been developed (Patent Document 2), and Patent Document 3 discloses a Pseudomonas fluorescens G7090 strain having a controlling effect against a rot disease (Pseudomonas cichorii) which is a bacterial disease of lettuce. As Pseudomonas azotoformans, the F30A strain is described in Patent Document 4, and the S4 strain is described in Non-Patent Document 1 as having activity against rice banana seedling disease. It is described in Non-Patent Document 2 that the strain GC-B19 has activity against cucumber anthracnose, and NBRC12693 strain is Non-Patent Document 3 (in the literature, strains are Iizuka and Komagata F-3. Pseudomonas azotoformans are described in Patent Documents 5 to 7 as an example of a bacterium. Of these Pseudomonas azotoformans, only two strains, S4 strain and GC-B19 strain, are known to have the ability to control plant diseases.

However, these microbial pesticides generally target plant diseases to a small part of bacterial diseases and filamentous fungal diseases, and are not very effective against other bacterial diseases and most filamentous fungal diseases. I can't expect it. Therefore, there is a demand for the development of microbial pesticides that are effective against a variety of diseases, practical as pesticides, and highly active.

WO2008/056653A Brochure JP, 11-302120, A JP 2001-247423 A Japanese Patent Publication No. 2013-514805 Japanese Patent Publication No. 2016-528893 JP, 2005-164429, A Special table 2013-511276 gazette "Journal of Biotechnology", April 11, 2016, Issue 227, p.25-26 "PHYTOPATHOLOGY", (USA), January 29, 2014, Volume 104, Issue 8, p. 834-842 "Journal of the Japanese Society of Agricultural Chemistry", July 23, 1963, Volume 37, Issue 3, p.137-141

An object of the present invention is to provide a novel microorganism that has a low load on the environment, has a very low possibility of generating a resistant pathogenic bacterium, and has an excellent control ability against various plant diseases. is there. In particular, the present invention is to provide a novel microorganism that not only has a practical effect on filamentous fungal diseases, but can also exhibit a high pest control effect as a pesticide against both bacterial diseases and filamentous fungal diseases.

In order to solve the above problems, the present inventors collected microorganisms from various plants, cultured and isolated them, and searched for highly active microorganisms. As a result, they have found a microorganism having a high control effect against various plant diseases including lettuce soft rot. When the mycological properties of this microorganism and the nucleotide sequence of the 16S rDNA gene were examined, it was found to be a new strain of Pseudomonas azotoformans.

That is, the present invention relates to the following inventions.
(1) Pseudomonas azotoformans W-14-1 strain NITE BP-02371, or a mutant strain thereof.
(2) A method for controlling plant diseases, which comprises treating Pseudomonas azotoformans W-14-1 strain NITE BP-02371 or a mutant strain thereof on a plant and/or cultivated soil of the plant.
(3) Pseudomonas azotoformans W-14-1 strain NITE BP-02371, or a mutant thereof, and a plant disease control agent containing a surfactant.
(4) Pseudomonas azotoformans strain W-14-1 strain NITE BP-02371 or a mutant strain thereof is contained in an amount of 0.01 to 30 parts by mass with respect to 1 part by mass of the surfactant. ) The plant disease control agent according to [4].

The microorganism of the present invention has a low load on the environment, has a very low possibility of generating resistant pathogenic bacteria, and has an excellent controlling ability for various plant diseases for a longer period of time. Further, it is possible to show a controlling effect against both bacterial diseases and filamentous fungal diseases.

It is a figure which shows the phylogenetic tree of Pseudomonas azotoformans strain W-14-1 (Pseudomonas azotoformans NITE BP-02371) of this invention produced based on the nucleotide sequence of 16S rDNA gene.

<1> Microorganism of the present invention The microorganism of the present invention is a pseudomonas azotoformans (Pseudomonas azotoformans) W-14-1 strain having the ability to control plant diseases, and naturally from the strain without artificial manipulation. Or a mutant strain obtained by an artificial mutagenesis operation from the strain, which mutant has the ability to control plant diseases.
“Having the ability to control plant diseases” means having an antagonistic action against pathogenic bacteria of diseases of any plant. The microorganism of the present invention prevents or cures a plant disease caused by the pathogenic bacterium by exerting an antagonistic action against the pathogenic bacterium of the plant disease, and is particularly excellent in the effect of preventing the plant disease.
The term "prevent plant diseases" means that plants that are not infected with pathogenic bacteria of plant diseases or show no symptom, or cultivated soil thereof, are treated under the same suitable conditions except that the microorganism of the present invention is treated. When cultivated, it means that the plant treated with the microorganism of the present invention has a lower degree of disease than the plant not treated with the microorganism of the present invention.
“Healing a disease of a plant” means that when a plant in which pathogenic bacteria of a plant disease is infected and a symptom appears, is cultivated under the same suitable conditions except that the microorganism of the present invention is treated, The plant treated with the microorganism of the present invention has a lower degree of disease than the plant not treated with the microorganism.
“Low degree of disease” means, for example, that the degree of disease (or rate) is low and the control value is greater than 0. The control value is preferably a larger value, excellent when 30 or more, more excellent when 50 or more, and particularly excellent when 60 or 70 or more.

Pseudomonas Azotoformans W-14-1 strain was dated October 12, 2016, National Institute for Product Evaluation Technology, Patent Microorganism Depositary Center (NPMD) (2-5-8 Kazusa Kamasa, Kisarazu City, Chiba Prefecture). No.) has been internationally deposited under the international deposit number NITE BP-02371.

The mycological properties of Pseudomonas azotoformans strain W-14-1 (hereinafter referred to as "the present microorganism"), which is the microorganism of the present invention, are as follows.
This microorganism is a Gram-negative bacillus, does not form spores, has a total cell length of 1.5 to 2.5 μm and a total width of about 0.8 μm, and has motility. It shows rough irregular colonies on standard agar and produces fluorescent dye on King B medium. No growth was observed at 41° C., catalase activity was positive, OF medium test was negative for oxidation, nitrate reduction was negative, indole production was negative, urease activity was negative, gelatin degradation was positive, β-galactosidase activity was negative, β-glucosidase activity is negative. In the LOPAT test, levan production was positive, potato tuber rot was negative, tobacco hypersensitivity reaction was negative, oxidase activity was positive, and arginine degradation was positive. Starch assimilability is negative, and as for assimilation of carbon compounds such as sugars and organic acids, D-glucose is positive, L-arabinose is positive, D-mannose is positive, D-mannitol is positive, N- Acetyl-D-glucosamine is positive, maltose is negative, potassium gluconate is positive, n-capric acid is positive, adipic acid is negative, DL-malic acid is positive, sodium citrate is positive, phenyl acetate is negative, sucrose Is positive, trehalose is positive, ribitol (adnit) is positive, sorbitol is positive, butyric acid is negative, propionic acid is positive, and propylene glycol is negative.

The measuring method of each item is not particularly limited, and a generally known method can be used. For example, the method described in Patent Document 1 can be used.

The microorganism can be obtained by culturing the microorganism after isolation using the 16S rDNA gene sequence (see SEQ ID NO: 3) and/or the above-mentioned mycological properties of the strain as an index.

The present microorganism is a microbial cell of any form (for example, dormant cells) shown by a viable bacterium of Pseudomonas azotoformans W-14-1 strain, including vegetative cells of Pseudomonas azotoformans strain W-14-1. May be

(Culturing method)
The method for culturing the present microorganism is not particularly limited, but in the case of solid culture, for example, a method of static culture at 20 to 35° C. using a standard agar medium, an ordinary agar medium, a potato dextrose agar medium, or the like can be mentioned. In the case of liquid culture, there may be mentioned a method of shaking and stirring culture at 20 to 35° C. using various liquid culture media and the like obtained by removing agar from the agar culture medium.

(Plant disease)
This microorganism can be used for controlling plant diseases derived from bacteria belonging to obligate aerobic bacteria, microaerobic bacteria, facultative anaerobic bacteria, etc.,
It can be used to control plant diseases derived from filamentous fungi such as algal fungi (Oomycetes), ascomycetes (Ascomycetes), incomplete fungi (Deuteromycetes), basidiomycetes (Basidiomycetes), and zygomycetes (Zygomycetes). ..
For example, the microorganism is soft rot, rot, spot bacterial disease, leaf spot bacterial disease, ulcer disease, perforation bacterial disease, black spot bacterial disease, brown spot bacterial disease, stem nematode bacterial disease, rice blast bacterial disease, seedlings. Bacterial diseases such as bacterial wilt, bacterial wilt, white leaf blight, black rot, burns, etc.
Gray mold, aspergillus, sclerotium, brown spot, scab, spotted leaf spot, black spot, summer blight, ring spot, leaf mold, leaf scab, scab, anthrax, udon It has the ability to control fungal diseases such as mildew, rust, black spot, plague, and downy mildew. However, the target of control by the present microorganism is not limited to these.

In addition, examples of plant diseases (pathogens) to be controlled are shown below.
Sugar beet: brown spot (Cercospora beticola), black root (Aphanomyces cochlioides), root rot (Thanatephorus cucumeris), leaf rot (Thanatephorus cucumeris), etc. Peanut: Mycosphaerella arachidis, spot blotch (Ascochyta sp. ), rust disease (Puccinia arachidis), wilt disease (Pythium debaryanum), rust spot disease (Alternaria alternata), white silkworm disease (Sclerotium rolfsii) black astringent disease (Mycosphaerella berkeleyi) cucumber: powdery mildew (Sphaerotheca fuliginea), Downy mildew (Pseudoperonospora cubensis), wilt blight (Mycosphaerella melonis), wilt disease (Fusarium oxysporum), sclerotinia (Sclerotinia sclerotiorum), gray mold (Botrytis cinerea), anthracnose (Colletotrichum orbiculare), scab (Cladosporium cucumerinum), brown spot (Corynespora cassiicola), seedling blight (Pythium debaryanum, Rhizoctonia solani Kuhn), Phomopsis root rot (Phomopsis sp.) Spot bacterial disease (Pseudomonas syringae pv. Lechrymans), etc. Disease (Botrytis cinerea), leaf mold (Cladosporium fulvum), plague (Phytophthora infestans), half body wilt (Verticillium albo-atrum, Verticillium dahliae), powdery mildew (Oidium neolycopersici), ring spot (Alternaria solani), Mold (Pseudocercospora fuligena) etc. Eggplant: Gray mold (Botrytis cinerea), Black blight (Corynespora melongenae), Powdery mildew (Erysiphe cichoracearum), Botrytis cinerea (Myc) Strawberry: Botrytis cinerea, powdery mildew (Sphaerotheca humuli), anthracnose (ovellosiella nattrassii), sclerotinia sclerotiorum, half body wilt (Verticillium dahliae), brown spot (Phomopsis vexans), etc. Colletotrichum acutatum, Colletotrichum fragariae), plague (Phytophthora cactorum), soft rot (Rhizopus stolonifer), yellow rot (Fusarium oxysporum), wilt (Verticillium dahliae), etc. Onion: Gray rot (Botrytis cine alli), Gray rot (Botrytis allii) ), white spot blight (Botrytis squamosa), downy mildew (Peronospora destructor), white plague (Phytophthora porri), etc. Cabbage: root-knot disease (Plasmodiophora brassicae), soft rot (Erwinia carotovora), black rot (Xanthomonas campesrtis pv) campestris), black spot bacterial disease (Pseudomonas syringae pv. maculicola, P. s. pv. alisalensis), downy mildew (Peronospora parasitica), sclerotinia sclerotiorum, black scab (Alternaria brassicicola), gray mold Diseases (Botrytis cinerea), etc. Lettuce: Soft rot (Erwinia carotovora), Spot bacterial disease (Xanthomonas axonopodis pv. vitians), etc. Chinese cabbage: Soft rot (Erwinia carotovora), etc. Green beans: Sclerotinia sclerotiorum, Gray mold (Botrytis) cinerea), anthrax (Colletotrichum lindemuthianum), horn spot (Phaeoisariopsis griseola), etc.

Apples: Powdery mildew (Podosphaera leucotricha), scab (Venturia inaequalis), monilia (Monilinia mali), sunspot (Mycosphaerella pomi), rot (Valsa mali), leaf spot (Alternaria mali), red scab (Gymnosporangium). yamadae), leaf spot (Botryosphaeria berengeriana), anthracnose (Glomerella cingulata, Colletotrichum acutatum), brown spot (Diplocarpon mali), soot spot (Zygophiala jamaicensis), spot blotch (Gloeodes pomigena), purple leaf spot. (Helicobasidium mompa), gray mold (Botrytis cinerea), etc. Plum: Cladosporium carpophilum, gray mold (Botrytis cinerea), sterility (Monilinia mumecola), etc. Oyster: powdery mildew (Phyllactinia kakicola), anthracnose Diseases (Gloeosporium kaki), leaf spots (Cercospora kaki), etc. Peach: Monilinia fructicola, scab (Cladosporium carpophilum), Phomopsis rot (Phomopsis sp.), perforation bacterial disease (Xanthomonas campestris pv. pruni) ) Almonds: Monilinia laxa, Spot disease (Stigmina carpophila), Black spot disease (Cladosporium carpophilum), Leaf blight (Polystigma rubrum), Leaf spot disease (Alternaria alternata), Anthrax (Colletotrichum gloeospoides), etc. Sweet cherry: Monilinia fructicola, Anthracnose (Colletotrichum acutatum), Black spot (Alternaria sp.), Sclerosis (Monilinia kusanoi), etc. Grape: Gray mold (Botrytis cinerea), powdery mildew (Uncinula necato r), late rot (Glomerella cingulata, Colletotrichum acutatum), downy mildew (Plasmopara viticola), black rot (Elsinoe ampelina), brown spot (Pseudocercospora vitis), black rot (Guignardia bidwellii), white rot ( Coniella castaneicola), rust (Phakopsora ampelopsidis), etc. Pear: scab (Venturia nashicola), scab (Gymnosporangium asiaticum), black spot (Alternaria kikuchiana), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia). , Blight (Phomopsis fukushii), brown leaf spot (Stemphylium vesicarium), anthracnose (Glomerella cingulata), etc. Cha: ring spot (Pestalotiopsis longiseta, P. theae), anthracnose (Colletotrichum theae-sinensis), net Rice blast (Exobasidium reticulatum), etc. Citrus: Scab (Elsinoe fawcettii), Blue mold (Penicillium italicum), Green mold (Penicillium digitatum), Gray mold (Botrytis cinerea), Black spot disease (Diaporthe citri), Canker disease (Diaporthe citri). Xanthomonas campestris pv.Citri), powdery mildew (Oidium sp.), etc.

Wheat: Powdery mildew (Blumeria graminis f.sp. tritici), Fusarium head blight (Gibberella zeae), Leaf rust (Puccinia recondita), Brown snow rot (Pythium iwayamai), Red snow rot (Monographella nivalis), Eye print Disease (Pseudocercosporella herpotrichoides), leaf blight (Septoria tritici), wilt disease (Leptosphaeria nodorum), snow rot small grain sclerot (Typhula incarnata), snow rot large grain sclerotia (Myriosclerotinia borealis), blight (Gaeumannomyces graminis). ), ergot (Claviceps purpurea), linseed smut (Tilletia caries), naked scab (Ustilago nuda), etc. Barley: leaf spot (Pyrenophora graminea), net blotch (Pyrenophora teres), cloudy leaf disease (Rhynchosporium secalis), Naked smut (Ustilago tritici, U.nuda), etc. Rice: blast (Pyricularia oryzae), blight (Rhizoctonia solani), scabbard seedling (Gibberella fujikuroi), sesame leaf blight (Cochliobolus miyabeanus), seedling blight (Pythium graminicola), White leaf blight (Xanthomonas oryzae), Seedling bacterial wilt (Burkholderia plantarii), Brown streak (Acidovorax avenae), Bacterial bacterial blight (Burkholderia glumae), Streaked leaf blight (Cercospora oryzae), Rice Koji disease (Ustilaginoidea virens), brown rice (Alternaria alternata, Curvularia intermedia), black rice (Alternaria padwickii), red rice (Epicoccum purpurascens), etc. Tobacco: Sclerotinia sclerotiorum, powdery mildew (Erysiphe cichoracearum), Plague (Phytophthora nicotianae ), etc. Tulip: Gray mold (Botrytis cinerea), etc. Sunflower: Downy mildew (Plasmopara halstedii), Sclerotinia sclerotiorum, etc. Shiva: Snow rot large sclerotinia borealis (Sclerotinia borealis), large patch (Rhizoctonia solani) , Brown patch (Rhizoctonia solani), dollar spot (Sclerotinia homoeocarpa), blast (Pyricularia sp.), red blight (Pythium aphanidermatum), anthracnose (Colletotrichum graminicola), etc. Orchardgrass: powdery mildew (Erysiphe graminis), etc. Soybean: Purpura (Cercospora kikuchii), downy mildew (Peronospora manshurica), stem sickness (Phytophthora sojae), rust (Phakopsora pachyrhizi), sclerotinia sclerotiorum, anthracnose (Colletotrichum truncatum), gray mold (Botrytis cinerea), black scab (Elsinoe glycines), scab (Diaporthe phaseolorum var. sojae), etc. Potato: Phytophthora infestans, summer blight (Alternaria solani), black blight (Thanatephorus cucumeris), wilt wilt. Verticillium albo-atrum, V. dahliae, V. nigrescens, etc. Banana: Panama disease (Fusarium oxysporum), Sigatoka disease (Mycosphaerella fijiensis, M. musicola), etc. Rape: Sclerotinia sclerotiorum, root rot (Phoma lingam) ), Black spot (Alternaria brassicae), etc. Coffee: Rust (Hemileia vastatrix), Anthrax (Colletotrichum coffeanum), Brown eye (Cercospora coffeicola), etc. Sugar cane: Brown rust (Pucc) Inia melanocephala, etc. Maize: Hail blotch (Gloeocercospora sorghi), Rust (Puccinia sorghi), Southern rust (Puccinia polysora), Black scab (Ustilago maydis), Sesame leaf blight (Cochliobolus heterostrophus), Stem blotch (Setosphaeria) turcica) Cotton: Seedling blight (Pythium sp.), rust (Phakopsora gossypii), white mold (Mycosphaerella areola), anthrax (Glomerella gossypii), etc.

(Applicable plants)
The plant to which the present microorganism is applied is not particularly limited as long as the plant can exert its controlling ability.
The present microorganism can be used for plants such as cereals; vegetables; root vegetables; potatoes; trees such as fruit trees, tea, coffee, cacao; grasses; grasses; cotton.

For example, Brassicaceae, Solanaceae, Cucurbitaceae, Liliaceae, Leguminaceae, Asteraceae, Acanthaceae, Gramineae, Rosaceae, Dianthaceae, Primroseaceae, Rutaceae, Vineaceae, Matathidae, Oysteraceae, Seriaceae, The plants belonging to the family Convolvulaceae or the family Araceae can be mentioned. Among them, plants belonging to the family Cruciferae such as Chinese cabbage, plants belonging to the Asteraceae family such as lettuce, plants belonging to the Solanaceae family such as potato, mandarin oranges such as lemon and navel Preference is given to plants belonging to the family, plants belonging to the family Rosaceae such as pear, and the like.

The present microorganism can be applied to various parts of plants such as leaves, stems, stalks, flowers, buds, fruits, seeds, sprouts, roots, tubers, tuberous roots, shoots and cuttings. Further, improved varieties and varieties of these plants, cultivated varieties, mutants, hybrids, and genetically modified organisms (GMO) can also be targeted.

This microorganism is used for seed treatment (Seed treatment), seed potato treatment (Seed tuber treatment), foliar spraying, soil application, water surface treatment to control various diseases that occur in agricultural and horticultural crops including flowers, turf and grass. It can be used for application.

<2> Plant Disease Control Agent Containing the Present Microorganism A plant disease control agent containing the present microorganism is particularly preferable as long as it contains cells of Pseudomonas azotoformans W-14-1 strain or a mutant strain thereof. Not limited. Hereinafter, the “present control agent” refers to a control agent for plant diseases containing the present microorganism.

(shape)
The microorganism may be any of the microorganism of the microorganism itself, a suspension containing the microorganism, a culture solution containing the microorganism, or a culture thereof, a concentrate, a paste, a dried product, a diluted product, or the like. Even in the form, it can be used in the present control agent.

(Prevention, cure)
The present control agent can prevent or cure a plant disease caused by the pathogenic bacterium by causing the microorganism to exert an antagonistic action against the pathogenic bacterium of the plant disease. The present control agent can be used as a preventive agent for plant diseases or a therapeutic agent for plant diseases, but is particularly excellent in effect as a preventive agent for plant diseases.

(Dilution concentration)
The concentration of the present microorganism contained in the present control agent is not particularly limited as long as the effect of the present invention is not impaired. For example, when the present control agent is diluted 1000 to 2000 times, the concentration of the microorganism is 1 It can be preferably mentioned that the concentration is within a range of ×10 ² to 1×10 ¹¹ cfu/ml, preferably 1×10 ⁴ to 1×10 ⁹ cfu/ml.

(Additive)
The present control agent may contain optional components in addition to the present microorganism as long as the effects of the present invention are not impaired. The optional components are not particularly limited as long as they do not impair the effects of the present invention, and examples thereof include carriers, surfactants, dispersants, and auxiliary agents. Particularly, it is preferable to add at least a surfactant. Further, if necessary, an antioxidant, a colorant, a lubricant, an ultraviolet absorber, an antistatic agent, a preservative and the like can be added.
Further, chemical pesticides such as fungicides, insecticides, herbicides, growth regulators, etc. can be mixed within a range that does not affect the present microorganism. Further, even chemical pesticides such as bactericides, insecticides, herbicides, growth regulators, etc., which affect the present microorganisms can be used at intervals of several days.

(Content of this microorganism)
The content of the present microorganism is not particularly limited, but is 0.001 to 99 parts by mass, preferably 0.01 to 80 parts by mass, and more preferably 0.1 to 100 parts by mass of the present control agent. 70 parts by mass, more preferably 1 to 50 parts by mass.

(Additive: carrier)
As the carrier, inorganic salts such as calcium carbonate, potassium chloride, sodium sulfate, calcium sulfate, ammonium sulfate; organic acids such as citric acid, malic acid, stearic acid and salts thereof; sugars such as glucose, lactose, sucrose; alumina. Solid carrier such as powder, silica gel, zeolite, hydroxyapatite, zirconium phosphate, titanium phosphate, titanium oxide, zinc oxide, hydrotalcite, kaolinite, montmorillonite, talc, clay, diatomaceous earth, bentonite, white carbon, kaolin, vermiculite Can be mentioned.
The content of the carrier is not particularly limited, but the mixing ratio of the carrier is 0.01 to 30 parts by mass, preferably 0.1 to 20 parts by mass, relative to 1 part by mass of the microorganism. It is preferably 0.3 to 10 parts by mass.

(Additive: surfactant or dispersant)
The surfactant (which can also be used as a dispersant) is not particularly limited as long as it can be used in ordinary agricultural and horticultural preparations, and specifically, the following nonionic surfactants and anions are used. There are cationic surfactants, cationic surfactants, and amphoteric surfactants.
As the nonionic surfactant, sugar ester type surfactants such as sorbitan fatty acid ester (C12-18), POE sorbitan fatty acid ester (C12-18), sucrose fatty acid ester; POE fatty acid ester (C12-18), Fatty acid ester type surfactants such as POE resin acid ester and POE fatty acid diester (C12-18); alcohol type surfactants such as POE alkyl ether (C12-18); POE alkyl (C8-12) phenyl ether, POE dialkyl Alkylphenol type surfactants such as (C8-12) phenyl ether and POE alkyl (C8-12) phenyl ether formalin condensate; polyoxyethylene/polyoxypropylene block polymer; alkyl(C12-18) polyoxyethylene/polyoxy Polyoxyethylene/polyoxypropylene block polymer type surfactants such as propylene block polymer ether; alkylamine type surfactants such as POE alkylamine (C12-18) and POE fatty acid amide (C12-18); POE fatty acid bisphenyl Bisphenol type surfactants such as ethers; Polyaromatic ring type surfactants such as POA benzyl phenyl (or phenyl phenyl) ethers and POA styryl phenyl (or phenyl phenyl) ethers; POE ether and ester type silicone and fluorine type surfactants And other silicone-based and fluorine-based surfactants; vegetable oil-type surfactants such as POE castor oil and POE hydrogenated castor oil; and the like.

As the anionic surfactant, alkyl sulfate (C12-18, Na, NH4, alkanolamine), POE alkyl ether sulfate (C12-18, Na, NH4, alkanolamine), POE alkylphenyl ether sulfate (C12-18, Sulfate such as NH4, alkanolamine), POE benzyl (or styryl)phenyl (or phenylphenyl) ether sulfate (Na, NH4, alkanolamine), polyoxyethylene, polyoxypropylene block polymer sulfate (Na, NH4, alkanolamine) Type surfactants: paraffin (alkane) sulfonate (C12-22, Na, Ca, alkanolamine), AOS (C14-16, Na, alkanolamine), dialkylsulfosuccinate (C8-12, Na, Ca, Mg) , Alkylbenzene sulfonate (C12, Na, Ca, Mg, NH4, alkylamine, alkanol, amine, cyclohexylamine), mono- or dialkyl (C3-6) naphthalene sulfonate (Na, NH4, alkanolamine, Ca, Mg), naphthalene sulfonate -Formalin condensate (Na, NH4), alkyl (C8-12) diphenyl ether disulfonate (Na, NH4), lignin sulfonate (Na, Ca), POE alkyl (C8-12) phenyl ether sulfonate (Na), POE alkyl ( C12-18) Sulfonate type surfactant such as ether sulfosuccinic acid half ester (Na); carboxylic acid type fatty acid salt (C12-18, Na, K, NH4, alkanolamine), N-methyl-fatty acid sarcosinate (C12-18). , Na), resinate (Na, K), etc. POE alkyl (C12-18) ether phosphate (Na, alkanolamine), POE mono- or dialkyl (C8-12) phenyl ether phosphate (Na, alkanolamine), POE benzyl (Or styryl) phenyl (or phenylphenyl) ether phosphate (Na, alkanolamine), polyoxyethylene/polyoxypropylene block polymer (Na, alkanolamine), phosphatidylcholine/phosphatidylethanolimine (lecithin), alkyl (C8-12) ) Phosphate-type surfactants such as phosphates; and the like.

Examples of the cationic surfactant include alkyl trimethyl ammonium chloride (C12-18), methyl polyoxyethylene alkyl ammonium chloride (C12-18), alkyl N-methylpyridinium bromide (C12-18), mono- or dialkyl. Ammonium-type surfactants such as (C12-18) methylated ammonium chloride and alkyl (C12-18) pentamethylpropylenediamine dichloride; alkyldimethylbenzalkonium chloride (C12-18), benzethonium chloride (octylphenoxyethoxyethyldimethylbenzyl) Ammonium chloride) and other benzalkonium-type surfactants; and the like.

Examples of the amphoteric surfactant include betaine-type surfactants such as dialkyl(C8-12)diaminoethylbetaine and alkyl(C12-18)dimethylbenzylbetaine; dialkyl(C8-12)diaminoethylglycine, alkyl(C12-18). Glycine type surfactants such as dimethylbenzylglycine; and the like.

The surfactants and/or dispersants can be used alone or in combination of two or more.
The content of the surfactant and/or the dispersant is not particularly limited, but the mixing ratio of the surfactant is 0.01 to 30 parts by mass relative to 1 part by mass of the present microorganism, and preferably 0. It is 1 to 20 parts by mass, more preferably 0.3 to 10 parts by mass.

(Additive: auxiliary agent)
Examples of the auxiliary agent include carboxymethyl cellulose, polyethylene glycol, gum arabic, polyvinylpyrrolidone, starch and the like.

(Formulation form)
The form of the present control agent is not particularly limited, and forms that can be taken by usual agricultural and horticultural agents, for example, powder (DP, Dustable Powder), wettable powder (WP, Wattable Powder), emulsion (EC, Emulsifiable Concentrate) Flowable agent (FL, flowable), suspension agent (SC, Suspension Concentrate), water solvent (SP, Water Soluble Powder), granule wettable powder (WG, Water Dispersible Granule), tablet (Tablet), granule (GR, Granule), SE agent (Suspo Emulsion), OD agent (Oil Dispersion), EW agent (Emulsion oil in water) and the like can be adopted. The method for preparing the preparation is not particularly limited, and a known preparation method can be adopted depending on the dosage form.
An example of the formulation of the present microorganism is shown below.

(Formulation 1: wettable powder)
40 parts of the present microorganism, 53 parts of diatomaceous earth, 4 parts of higher alcohol sulfate, and 3 parts of alkylnaphthalene sulfonate are uniformly mixed and finely pulverized to obtain a wettable powder having an active ingredient of 40%.

(Formulation 2: emulsion)
30 parts of the present microorganism, 33 parts of xylene, 30 parts of dimethylformamide, and 7 parts of polyoxyethylene alkylallyl ether were mixed and dissolved to obtain an emulsion containing 30% of the active ingredient.

(Formulation 3: Granule)
This microorganism 5 parts Talc 40 parts Clay 38 parts Bentonite 10 parts and sodium alkylsulfate 7 parts are uniformly mixed and finely pulverized, and then granulated into granules having a diameter of 0.5 to 1.0 mm to obtain an active ingredient of 5%. To obtain granules.

(Formulation 4: Granule)
This microorganism 5 parts Clay 73 parts Bentonite 20 parts Dioctyl sulfosuccinate sodium salt 1 part and potassium phosphate 1 part are well pulverized and mixed, water is added and well kneaded, then granulated and dried to obtain an active ingredient of 5%. To obtain granules.

(Formulation 5: Suspension)
This microorganism 10 parts Polyoxyethylene alkyl allyl ether 4 parts Polycarboxylic acid sodium salt 2 parts Glycerin 10 parts Xanthan gum 0.2 parts and water 73.8 parts are mixed and wet pulverized until the particle size becomes 3 microns or less, A suspension with 10% active ingredient is obtained.

(Plant Diseases and Applicable Plants of the Control Agent) The plant diseases and applicable plants of the present control agent are the same as the plant diseases and applicable plants of the present microorganism.

<3> Method for controlling plant disease with the present control agent The method for controlling plant disease with the present control agent is particularly limited as long as it is a method for treating the plant and/or cultivated soil of the plant with the present control agent. Instead, as in the case of using a normal chemical pesticide, it can be appropriately selected depending on the type of plant disease and the type of applied plant.

(Spraying method)
For example, the present control agent may be treated on a plant by directly applying or spraying the control agent onto a plant, or the present control agent may be mixed with soil for cultivating a plant (plant cultivation soil), The present control agent may be applied to the plant cultivation soil by spraying or irrigation. When the present control agent is applied to a cultivated soil of a plant, the present control agent may be applied to the soil and then a plant may be planted, or the plant may be applied to the soil and then the present control agent may be applied to the soil. .. Further, as described in JP-A-2001-302407, it is possible to dispose the pesticide in the vicinity of the air vent of the air blower that blows air into the facility, and to spray the pesticide with the air sent from the air vent. Further, the present control agent can be used to coat, dress, or soak the seeds or tubers of plants, or to dip the roots of plant seedlings for treatment.

(Soil application concentration)
When the present control agent is applied to plants and/or cultivated soil of the plants, the control agent can be used as it is, or can be used after diluting it with an appropriate amount of water or the like. .. The amount of the present control agent to be applied to plants and/or cultivated soil of the plants cannot be unconditionally specified because it varies depending on the type of plant disease, the type of applied plant, etc. The cell concentration may be in the range of usually 1×10 ² to 1×10 ¹¹ cfu/ml, preferably 1×10 ⁴ to 1×10 ⁹ cfu/ml.

Other components of the present control agent include fungicides, insecticides, acaricides, nematicides, soil pesticides, plant regulators, synergists, fertilizers, soil conditioners, animal feeds, etc. It can be mixed or mixed. By containing such other components, a synergistic effect may be exhibited.

Specific examples of the bactericide which can be mixed or used together with the present control agent are shown below.
(1) Nucleic acid biosynthesis inhibitor:
(a) RNA polymerase I inhibitor: benalaxyl (benalaxyl), benalaxyl-M (benalaxyl-M), furalaxyl (furalaxyl), metalaxyl (metalaxyl), metalaxyl-M (metalaxyl-M), oxadixyl (oxadixyl), blackzillacon (clozylacon) ), offurace;
(b) Adenosine deaminase inhibitor: bupirimate, dimethirimol, ethirimol;
(c) DNA/RNA synthesis inhibitor: hymexazol, octhilinone;
(d) DNA topoisomerase II inhibitor: oxolinic acid.

(2) Antimitotic agent and antimitotic agent:
(a) β-tubulin polymerization inhibitor: benomyl, carbendazim, carbfenazim, chlorfenazole, fuberidazole, thiabendazole, thiophanate, thiophanate-methyl , Diethofencarb, zoxamide, ethaboxam;
(b) anti-mitotic agent: pencycuron;
(c) Delocalization inhibitor of spectrin-like protein: fluopicolide.

(3) Respiratory inhibitor:
(a) Complex I NADH oxidoreductase inhibitor: diflumetorim, tolfenpyrad;
(b) Complex II succinate dehydrogenase inhibitor: benodanil, flutolanil, flutolanil, mepronil, isofetamid, fluopyram, fenfuram, flumecyclox , Carboxin, oxycarboxin, oxycarboxin, thifluzamide, benzovindiflupyr, bixafen, fluxapyroxad, flamethopyr, furametpyr, isopyrazam, Penflufen, penthiopyrad, sedaxan, boscalid, pyraziflumid;
(c) Complex III Ubiquinol Oxidase Qo Inhibitor: Azoxystrobin (azoxystrobin), Cumoxystrobin (coumoxystrobin), Cumoxystrobin (coumethoxystrobin), Enoxastrobin (Enoxastrobin), Fluphenoxystrobin (Flufenoxystrobin) , Picoxystrobin, pyraoxystrobin, pyraclostrobin, pyrametostrobin, triclopyricarb, kresoxim-methyl, trifloxist Robin (trifloxystrobin), dimoxystrobin (dimoxystrobin), phenaminestrobin (fenaminstrobin), metominostrobin (metominostrobin), orysastrobin (orysastrobin), famoxadone (flumastrodone), fluoxastrobin (fluoxastrobin), fenamidone (fenamidone) , Pyribencarb, mandestrobin;
(d) Complex III ubiquinol reductase Qi inhibitor: cyazofamid, amisulbrom;
(e) Uncoupler for oxidative phosphorylation: binapacryl, meptyldinocap, dinocap, fluazinam, ferimzone;
(f) Oxidative phosphorylation inhibitors (inhibitors of ATP synthase): fentin acetate, fentin chloride, fentin hydroxide;
(g) ATP production inhibitor: silthiofam;
(h) Complex III: Qx (unknown) inhibitor of cytochrome bc1 (ubiquinone reductase): ametoctradin.

(4) Amino acid and protein synthesis inhibitors
(a) Methionine biosynthesis inhibitors: andoprim, cyprodinil, mepanipyrim, pyrimethanil;
(b) Protein synthesis inhibitor: blasticidin-S, kasugamycin, kasugamycin hydrochloride, streptomycin, oxytetracycline.

(5) Signal transduction inhibitor:
(a) Signal transduction inhibitors: quinoxyfen, proquinazid;
(b) MAP/histidine kinase inhibitor in osmotic signal transduction: fenpiclonil (fenpiclonil), fludioxonil (fludioxonil), clozolinate (chlozolinate), iprodione (procymidone), vinclozolin (vinclozolin).

(6) Lipid and cell membrane synthesis inhibitor:
(a) Phospholipid biosynthesis, methyltransferase inhibitors: edifenphos, iprobenfos, pyrazophos, isoprothiolane;
(b) Lipid peroxides: biphenyl, chloroneb, dichloran, dichloran, quintozene, tecnazene, tolclofos-methyl, etridiazole;
(c) Agents that act on cell membranes: iodocarb, propamocarb, propamocarb-hydrochloride, propamocarb-fosetylate, prothiocarb;
(d) Microorganisms that disrupt the cell membrane of pathogenic bacteria: Bacillus subtilis (bacillus subtilis), Bacillus subtilis strain QST713 (bacillus subtilis strain QST713), Bacillus subtilis strain FZB24, Bacillus subtilis MBI600 strain (bacillus subtilis) , Bacillus subtilis strain D747 strain, Bacillus amyloliquefaciens;
(e) Cell membrane-disturbing agent: melaleuca altemifolia (tea tree) extract.

(7) Cell membrane sterol biosynthesis inhibitor:
(a) C14 demethylation inhibitor in sterol biosynthesis: triforine (triforine), pyrifenox (pyrifenox), pyrisoxazole (pyrisoxazole), fenarimol (fenarimol), flurprimidol (flurprimidol), nuarimol (nuarimol), Imazalil, imazalil-sulphate, oxpoconazole fumarate, pefurazoate, prochloraz, triflumizole, viniconazole, azaconazole azaconazole), bitertanol (bitertanol), bromconazole (bromconazole), cyproconazole (cyproconazole), diclobutrazol (diclobutrazol), difenoconazole (difenoconazole), diniconazole (diniconazole), diniconazole-M (diniconazole-M), epoxy Conazole (epoxyconazole), etaconazole (etaconazole), fenbuconazole (fenbuconazole), fluquinconazole (fluquinconazole), flusilazole (flusilazole), flutriafol (flutriafol), fluconazole (furconazole), fluconazole-cis (furconazole-cis) , Hexaconazole, imibenconazole, imibenconazole, ipconazole, metconazole, microbutanil, myconbutanil, penconazole, propiconazole, propiconazole, fluquinconazole, simeconazole (Simeconazole), tebuconazole (tebuconazole), tetraconazole (tetraconazole), triadimefon (triadimefon), triadimenol (triadimenol), triticonazole (triti) conazole), prothioconazole (prothioconazole), voriconazole (voriconazole), mefentrifluconazole (mefentrifluconazole);
(b) Inhibitors of Δ14 reductase and Δ8→Δ7-isomerase in sterol biosynthesis:
Aldimorph, dodemorph, dodemorph acetate, fenpropimorph, tridemorph, fenpropidine, piperalin, spiroxamine;
(c) 3-ketoreductase inhibitors in C4 demethylation of sterol biosynthesis system: fenhexamid, fenpyrazamine;
(d) Squalene epoxidase inhibitor of sterol biosynthesis system: pyributicarb, naphthifin, terbinafine.

(8) Cell wall synthesis inhibition
(a) Trehalase inhibitor: validamycin;
(b) Chitin synthase inhibitors: polyoxins, polyoxorim;
(c) Cellulose synthase inhibitor: dimethomorph, flumorph, pyrimorph, benchavalicarb-isopropyl, iprovalicarb, tolprocarb, valifenarate ), mandipropamid.

(9) Melanin biosynthesis inhibitor
(a) Reductase inhibitors of melanin biosynthesis: fthalide, pyroquilon, tricyclazole;
(b) Dehydrase inhibitors of melanin biosynthesis: carpropamid, diclocymet, fenoxanil.

(10) Host plant resistance inducer:
(a) Agents that act on the salicylic acid synthesis pathway: acibenzolar-S-methyl;
(b) Others: probenazole, tiadinil, isothianil, laminarin, and knotweed extract (reynoutria sachalinensis extract).

(11) Agents of unknown action: cymoxanil, fosetyl-aluminium, phosphoric acid (phosphate), tecloftalam, triazoxide, fursulfamide flusulfamide), diclomedine (diclomezine), metasulfocarb (methasulfocarb), cyflufenamid (cyflufenamid), metrafenone (metrafenone), pyriophenone (pyriofenone), dodine (dodine), dozine free base (dodine free base), flutianil (flutianil).

(12) Agents with multiple points of action: copper (copper salt), bordeaux mixture, copper hydroxide, copper naphthalate, copper oxide , Copper oxychloride, copper sulfate, sulfur, sulfur product, calcium polysulfide, ferbam, mancozeb, maneb , Mancopper, metiram, polycarbamate, propineb, thiram, zineb, ziram, captan, captafol, folpet (Folpet), chlorothalonil, dichlofluanid, tolylfluanid, guazatine, iminoctadine triacetate, iminoctadine trialbesilate, anilazine. , Dithianon, quinomethionate, fluoroimide.

(13) Other agents: DBEDC, fluorofolpet, guazatin acetate, bis(8-quinolinolato) copper(II), propamidine ( propamidine), chloropicrin, cyprofuram, agrobacterium, bethoxazin, diphenylamine, methyl isothiocyanate, mildeew-mycin ), capsaicin, cufraneb, cyprosulfamide, cyprosulfamide, dazomet, debacarb, dichlorophen, flumetover, fosetyl-calcium, fosetyl Sodium (fosetyl-sodium), irumamycin (irumamycin), natamycin (natamycin), nitrothal isopropyl (nitrothal isopropyl), oxamocarb (oxamocarb), pyrrolnitrin (pyrrolnitrin), tebufloquin (tebufloquin), tornifanide (zolylamide) zarilamide), algophase, amicalthiazol, oxathiapiprolin, metiram zinc, benthiazole, trichlamide, uniconazole, mildeomycin (Mildew-mycin), oxyfenthiin, picarbutrazox, fenpicoxamid, dichlobentiazox, quinofumelin, thiuram, ambam ), a Agrobacterium radiobacter, coniothyrium minitans, pseudomonas fluorescens, pseudomonas rhodesiae, non-thalassomyces flavus, talaromyces flavusder Carotovora (erwinia carotovora subsp. carotovora), bacillus simplex, variovorax paradoxus, lactobacillus plantarum.

Specific examples of insecticides, acaricides, nematicides, soil pesticides, anthelmintics and the like that can be mixed or used in combination with the present control agent are shown below.

(1) Acetylcholinesterase inhibitor:
(a) Carbamate system: alanycarb, aldicarb, bendiocarb, benfuracarb, butocarboxim, butoxycarboxim, carbaryl, carbofuran, carbofuran, Carbosulfan, ethiofencarb, fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, methomil, methomyl, oxamyl, pirimicarb. ), propoxur, thiodicarb, thiofanox, triazamate, trimethacarb, XMC, xylylcarb, phenothiocarb (fenothiocarb), MIPC, MPMC, MTMC, aldoxicarb (Aldoxycarb), alixycarb (aliyxycarb), aminocarb (aminocarb), bufencarb (clofenthocarb), cloethocarb, metam sodium (metam-sodium), promecarb (promecarb);

(b) Organophosphorus: acephate, azamethiphos, azinphos-ethyl, azinphos-methyl, cadusafos, chlorethoxyfos, chlorfenbin Phoss (chlorfenvinphos), chlormephos, chlorpyrifos, chlorpyrifos-methyl, coumaphos, cyanophos, demeton-S-methyl, diazinon ), dichlorvos/DDVP, dicrotophos, dimethoate, dimethoate, dimethylvinphos, disulfoton, EPN, ethion, ethoprophos, femfur, fenamiphos. fenamiphos), fenitrothion, fenthion, fosthiazate, heptenophos, imicyafos, isofenphos, isocarbophos, isocarbophos, isoxathion, malathion, malathion. mecarbam), methamidophos, methidathion, methidathion, mevinphos, monocrotophos, naled, omethoate, oxydimethone-oxydemeton-methyl, parathion, parathion. -Methyl (parathion-methyl), phenthoate, phorate, phosalone, phosmet, phosphamidon, phoxim, pirimiphos-methyl (p) irimiphos-methyl), profenofos, profenofos, propetamphos, prothiophos, pyraclofos, pyridaphenthion, quinalphos, quinalphos, sulfotep, tebupirinphos, tebupirimfos, tebupirimfos, tebupirimfos Terbufos, tetrachlorvinphos, thiometon, triazophos, trichlorfon, vamidothion, bromophos-ethyl, BRP, carbophenothion , Cyanofenphos, CYAP, demeton-S-methyl sulphone, dialifos, diclofenthion, dioxabenzophos, etrimfos, phensulfothion fensulfothion), flupyrazofos (flupyrazofos), honophos (fonofos), formothion (formothion), fosmethilan (fosmethilan), isazophos (isazofos), iodophenphos (jodfenphos), methacrifos (methacrifos), pirimiphos-ethyl (pirimiphos-bu). (Phosphocarb), propaphos, protoate, sulprofos.

(2) GABA-agonist chloride ion channel antagonists: acetoprole (acetoprole), chlordane, endosulfan, ethiprole, fipronil, pyrafluprole, pyriprole, pyriprole, Camphechlor, heptachlor, dienochlor.
(3) Sodium channel modulators: acrinathrin (acrinathrin), d-cis-trans allethrin (d-cis-trans allethrin), d-trans allethrin (d-trans allethrin), bifenthrin (biofenethrin), bioallethrin (bioallethrin), bio Allethrin s-cyclopentyl isomer, bioresmethrin, biopromethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin. -cyhalothrin), gamma-cyhalothrin (γ-cyhalothrin), cypermethrin (cypermethrin), alpha-cypermethrin (α-cypermethrin), beta-cypermethrin (β-cypermethrin), theta-cypermethrin (θ-cypermethrin), zeta -Cypermethrin, cyphenothrin [(1R)-trans isomer], deltamethrin, Empentrin [(EZ)-(1R)-isomer] (Empenthrin[(EZ)-(1R)-Isomer]), esfenvalerate, esfenvalerate, etofenprox, fenpropatrin, fenvalerate, flucythrinate, flumethrin (Flumethrin), tau-fluvalinate (τ-fluvalinate), halfenprox (halfenprox), imiprothrin, cadprothrin, kadethrin, permethrin, phenothrin [(1R)-trans isomer] (phenothrin [( 1R)-trans isomer]), prallethrin, pyrethrum, resmethrin, shirafluoff (Silafluofen), tefluthrin (tefluthrin), tetramethrin [(1R)-isomer] (tetramethrin [(1R)-isomer]), tralomethrin (translothrin), transfluthrin (allethrin), pyrethrin ( pyrethrins), pyrethrin I (pyrethrin I), pyrethrin II (pyrethrin II), profluthrin (profluthrin), dimefluthrin (dimefluthrin), bioethanomethrin (bioethanomethrin), biopermethrin (biopermethrin), transpermethrin (transpermethrin), (Fenfluthrin), fenpirithrin (fenpirithrin), flubrocythrinate (flubrocythrinate), flufenprox (flufenprox), metofluthrin (metofluthrin), protrifenbute (protrifenbute), pyrethmethrin (pyresmethrin), terrarethrin (terallethrin).

(4) Nicotinic acetylcholine receptor agonists: acetamiprid (acetamiprid), clothianidin (clothianidin), dinotefuran (dinotefuran), imidacloprid (imidacloprid), nitenpyram (nitenpyram), nithiazine (nithiazine), thiacloprid (thiacloprid), thiamethox Sulfoxaflor, nicotine, flupyradifurone.
(5) Nicotinic acetylcholine receptor allosteric modulators: spinetoram and spinosad.
(6) Chloride channel activator: abamectin, emamectin benzoate, lepimectin, milbemectin, ivermectin, seramectin, doramectin, doramectin, eprinomectin. (Eprinomectin), moxidectin (moxidectin), milbemycin (milbemycin), milbemycin oxime (milbemycin oxime), nemadectin (nemadectin).
(7) Juvenile hormone-like substances: hydroprene, kinoprene, kinoprene, methoprene, phenoxycarb, pyriproxyfen, diofenolan, epophenonane, triprene ).
(8) Other non-specific inhibitors: methyl bromide, chloropicrin, sulfuryl fluoride, borax, tartar emetic.
(9) Homoptera selective feeding inhibitors: flonicamid, pymetrozine, and pyrifluquinazon.

(10) Mite growth inhibitor: clofentezine, diflovidazin, hexythiazox, etoxazole.
(11) Insect mid-intestinal membrane-destroying agent derived from microorganisms: Bacillus thuringiensis subsp. israelensi, Bacillus thuringiensis subsp. isaelensi, Bacillus thuringiensis subsp. aizawai , Bacillus thuringtensis subsp. kurstaki, Bacillus thuringensis subsp. tenebrionis, Bt crop proteins: Cry1Ab, Cry1Ac, Cry1Fa, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, V3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A2, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A3, Cry1A. , Cry3Ab, Cry3Bb, Cry34Ab1/Cry35Ab1.
(12) Mitochondrial ATP biosynthetic enzyme inhibitor: diafenthiuron, azocyclotin, cyhexatin, fenbutatin oxide, propargite, tetradifon.
(13) Oxidative phosphorylation uncoupling agent: chlorfenapyr (chlorfenapyr), sulfuramid (sulfuramid), DNOC, binapacryl (binapacryl), dinobuton (dinobuton), dinocap (dinocap).
(14) Nicotinic acetylcholine receptor channel blocker: bensultap, cartap hydrochloride, nereistozin, thiosultap-sodium, thiocyclam.
(15) Chitin synthesis inhibitor: bistrifluron (bistrifluron), chlorfluazuron (chlorfluazuron), diflubenzuron (diflubenzuron), flucycloxuron (flucycloxuron), flufenoxuron (flufenoxuron), hexaflumuron (hexaflumuron), Lufenuron (lufenuron), novalron (novaluron), noviflumuron (noviflumuron), teflubenzuron (teflubenzuron), triflumuron (triflumuron), buprofezin (buprofezin), fluazuron (fluazuron).
(16) Diptera molting agent: cyromazine.
(17) Molting hormone receptor agonist: Chromafenozide, halofenozide, methoxyfenozide, tebufenozide.
(18) Octopamine receptor agonists: amitraz, demiditraz, chlordimeform.
(19) Mitochondrial electron transport complex III inhibitor: acequinocyl, fluacrypyrim, hydramethylnon.
(20) Mitochondrial electron transport complex I inhibitor: fenazaquin, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad, tolfenpyrad, rotenone.

(21) Voltage-dependent sodium channel blocker: indoxacarb, metaflumizone.
(22) Acetyl CoA carboxylase inhibitor: spirodiclofen, spiromesifen, spirotetramat.
(23) Mitochondrial electron transfer system complex IV inhibitors: aluminum phosphide (aluminium phosphide), calcium phosphide (calcium phosphide), phosphine (phosphine), zinc phosphide (zinc phosphide), cyanide (cyanide).
(24) Mitochondrial electron transport system complex II inhibitor: Cienopyrafen, cyflumetofen, and pyflubumide.
(25) Ryanodine receptor modulator: chlorantraniliprole, cyantraniliprole, cyantraniliprole, flubendiamide, cyclaniliprole, tetraniliprole.
(26) Mixed function oxidase inhibitor compound: piperonyl butoxide.
(27) Latrophilin receptor agonists: depsipeptide, cyclic depsipeptide, 24-membered cyclodepsipeptide, emodepside.
(28) Other agents (unknown mechanism of action): Azadirachtin, benzoximate, bifenazate, bromopropylate, quinomethionate, cryolite, dicofol. dicofol), pyridalyl, benclothiaz, sulfur, amidoflumet, 1,3-dichloropropene (1,3-dichloropropene), DCIP, phenisobromolate, benzomate. benzomate), metaldehyde, chlorobenzilate, clothiazoben, dicyclanil, fenoxacrim, fentrifanil, flubenzimine, fluphenazine. , Gossyplure, japonilure, metoxadiazone, petroleum (oil), potassium oleate (potassium oleate), tetrasul, triarathene, afidopyropene (afidopyropen), flomethokin (flometoquin), flometoquin (flometoquin), (flufiprole), fluensulfone, meperfluthrin (meperfluthrin), tetramethylfluthrin (tetramethylfluthrin), tralopyril (dimefluthrin), methylneodecanamide (methylneodecanamide), fluralaner (aforaolaner), afoxoranel Fluxametamide, 5-[5-(3,5-dichlorophenyl)-5-trifluoromethyl-4,5-dihydroisoxazol-3-yl]-2-(1H-1,2,4-triazole- 1-yl)benzonite Ril (CAS:943137-49-3) (5-[5-(3,5-dichlorophenyl)-5-trifluoromethyl-4,5-dihydroisoxazole-3-yl]-2-(1H-1,2,4- triazole-1-yl)benzonitrile (CAS:943137-49-3)), broflanilide, other metadiamides, Steinanema carpocapsae, Steinanema glaseri, pasteuria penetrans, Pekyromesis Paecilomyces tenuipes, paecilomyces fumosoroseus, beauveria bassiana, beauveria brongniartii, metalridium anisopriae le canti, varti

(29) Antiparasitic agent:
(a) Benzimidazole type: fenbendazole, albendazole, triclabendazole, triclabendazole, oxibendazole, mebendazole, oxfendazole, oxfendazole, perbendazole parbendazole), flubendazole (flubenzazole), febantel (febantel), netobimin (netobimin), thiophanate (thiophanate), thiabendazole (thiabendazole), cambendazole (cambendazole);
(b) Salicylanilide series: closantel, oxyclozanide, rafoxanide, niclosamide;
(c) Substituted phenol type: nitroxinil, nitroscanate;
(d) Pyrimidine type: pyrantel, morantel;
(e) Imidazothiazole system: levamisole, tetramisole;
(f) Tetrahydropyrimidines: praziquantel, epsiprantel;
(g) Other anthelmintic drugs: cyclodiene, ryania, chlorania, clorsulon, metronidazole, demiditraz, piperazine, diethylcarbamazine, diethylcarbamazine, dichlorophen. , Monepantel, tribendimidine, amidantel, thiacetalsamide, melarsomine, arsenamide.

Specific examples of plant regulators that can be mixed or used in combination with the present control agent are shown below.
Abscisic acid, kinetin, benzylaminopurine, 1,3-diphenylurea, 1,3-diphenylurea, forchlorfenuron, thidiazuron, chlorfenuron chlorfenuron), dihydrozeatin, gibberellin A, gibberellin A4, gibberellin A7, gibberellin A3, gibberellin A3, 1-methylcyclopropane, N -Acetylaminoethoxyvinyl glycine (also known as abiglycine), aminooxyacetate, silver nitrate, cobalt chloride, IAA, 4-CPA, cloprop (Cloprop), 2,4-D, MCPB, indole-3-butyrate, indole-3-butyrate, dichlorprop, phenothiol, 1-naphthyl acetamide, ethiclozet (Ethychlozate), cloxyfonac, maleic acid hydrazide, 2,3,5-triiodobenzoic acid (2,3,5-triiodobenzoic acid), salicylic acid (salicylic acid), methyl salicylate (methyl) salicylate), (-)-jasmonic acid ((-)-jasmonic acid), methyl jasmonate (methyl jasmonate), (+)-strigol ((+)-strigol), (+)-deoxystrigol ((+ )-deoxystrigol), (+)-Orobanchol ((+)-orobanchol), (+)-Sorgolactone ((+)-sorgolactone), 4-oxo-4-(2-phenylethyl)aminobutyric acid (4-oxo -4-(2-phen ylethyl)aminobutyric acid), ethephon, chlormequat, mepiquat chloride, benzyladenine, 5-amino levulinic acid, daminozide.

(Number of sprays)
The number of times of application of the present control agent can be appropriately selected depending on the type of plant disease, the type of applied plant, the degree of disease and the like.

(Use)
The present control agent can be mainly used for agriculture, but can also be used for other than agriculture. Examples of applications for agricultural use include seed treatment, seed potato disinfection, application to soil such as rice fields and fields, application to plants such as vegetables, fruits and rice, and examples of applications other than agriculture include bathroom. Examples thereof include a fungicide for wall surfaces of living rooms, a water quality improving agent for reservoirs, pools, cooling towers, sludge treating agents such as sludge, and the like.

(Spraying method)
This pesticide can be used without particular limitation on the spraying method, but it is diluted with water and sprayed to be sprayed, the solid agent is sprayed on the soil as it is, the solid agent is put into water and slowly released for effect. Can be exemplified.

Hereinafter, the present invention will be described more specifically with reference to Examples, but the technical scope of the present invention is not limited to these exemplifications.

[Example 1] Isolation of bacteria and identification 1. Isolation of candidate strains and selection of strains having plant disease control ability Chinese cabbage leaves collected in the field are homogenized in a small amount of sterilized water, and the resulting suspension is used as a standard agar medium (casein peptone 0.5% (w /V), yeast extract 0.25% (w/v), glucose 0.1% (w/v), agar content 1.5% (w/v), pH 6.9-7.1 after sterilization) ( It was smeared on a Nissui Pharmaceutical Co., Ltd.) and cultured at 25° C. for 2 days. Multiple grown single microbial colonies were collected and used as candidate strains. Among the thus-collected candidate strains, several strains having a control effect against the pathogenic bacterium of lettuce soft rot (Erwinia carotovora) in Test Example 1 were selected. With respect to the selected strains, in the field test of Test Example 2, a strain having a controlling effect against Chinese cabbage soft rot was further selected. The control value of the finally selected strain was 86.7 in Test Example 1 and 93.8 in Test Example 2. This selected strain was named W-14-1 strain.

[Test Example 1] Lettuce soft rot (bacterial disease) control effect test (indoor test)
A 300 ml Erlenmeyer flask was charged with 150 ml of a standard liquid medium (0.25% yeast extract, 0.5% peptone, 0.1% glucose, pH 7.0) and sterilized by heating. 0.1 ml of the culture to be tested was inoculated and cultivated in a reciprocating shaker at 30° C. and 100 rpm for 3 days. The obtained culture solution was centrifuged to remove the supernatant, and the precipitate was washed with water, and again centrifuged to remove the supernatant and the precipitate was washed with water. In this way, a bacterial cell suspension was prepared.
Next, this bacterial cell suspension was diluted with water to prepare a treatment liquid (A600 (absorbance at wavelength 600 nm)=0.1). The treatment liquid was sprayed on the cutouts of the cut leaves on the middle ribs of lettuce. Each sprayed lettuce was incubated at 28° C. for 5 hours in a humidified condition, and then an aqueous suspension (A600=0.05) of a lettuce soft rot pathogen (Erwinia carotovora) was added to each lettuce. The cutout was inoculated. After incubation at 28° C. for 4 days after inoculation, the degree of disease onset was investigated based on the following criteria, and the disease onset rate and control value were calculated based on the following equations 1 and 2.

Criterion 0: No lesion Criterion 1: Lesion is less than 10% of the central ribs Criterion 2: Lesion is less than 10-50% of the central ribs Criterion 3: Lesion is less than 50-75% of the central ribs Criterion 4: Lesion is Over 75% of midrib

(Formula 1)
Disease severity=((1×n1+2×n2+3×n3+4×n4)/(4×total number of investigated leaves))×100
n1 to n4 are the number of leaves corresponding to each of the criteria 1 to 4 (Equation 2)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

[Test Example 2] Chinese cabbage soft rot (bacterial disease) control effect test (field test)
A cell suspension of the culture to be tested was prepared in the same manner as in Test Example 1.
Next, this bacterial cell suspension was diluted with water to prepare a treatment liquid (A600=0.1). The treated solution was sprayed on Chinese cabbage planted in the field. The spraying treatment was performed twice at 1-week intervals. The causative agent of Chinese cabbage soft rot (Erbinia carotovora) was inoculated by spraying its aqueous suspension (A600=0.1) on the day of the first spraying treatment. One week after the second spraying, the degree of disease onset was investigated according to the following criteria, and the diseased strain rate, disease severity, and control value were calculated based on the following formulas 3 and 4.

Criterion 0: No disease occurrence Criterion 1: Criteria in which a part of the outer leaf is affected Criterion 2: Criteria in which a part of the outer leaf and head bulb are affected 3: Criteria are found in most of the head leaf

(Formula 3)
Disease severity=((1×n1+2×n2+3×n3)/(3×total number of surveyed strains))×100
n1 to n3 are the stock numbers corresponding to each of the criteria 1 to 3 (formula 4)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

2. Analysis of 16S rDNA Gene Regarding the W-14-1 strain, an attempt was made to amplify the 16S rRNA gene from the W-14-1 strain in order to analyze the nucleotide sequence of the 16S rRNA gene. Specifically, first, genomic DNA was isolated from the W-14-1 strain according to a conventional method. Using the obtained genomic DNA as a template, PCR amplification was carried out using 27F primer (SEQ ID NO: 1) and 1510R primer (SEQ ID NO: 2), which are often used for 16S rRNA gene amplification. The obtained PCR product was sequenced and the nucleotide sequence was decoded. Furthermore, assembly was performed based on the obtained nucleotide sequence information, and the full-length nucleotide sequence of the 16S rRNA gene was determined. The nucleotide sequence of the 16S rRNA gene of the W-14-1 strain is shown in SEQ ID NO:3.

A homology search was performed using BLAST based on the nucleotide sequence of the 16S rRNA gene of the W-14-1 strain. In addition, the nucleotide sequence of the 16S rRNA gene of the W-14-1 strain was analyzed using ClustalX, and the obtained analysis result was processed using Tree View to obtain a phylogenetic tree for the W-14-1 strain ( Figure 1) was created. This phylogenetic tree revealed the taxonomic position of the W-14-1 strain.
As a result, it was revealed that the W-14-1 strain is a bacterium belonging to the Pseudomonas fluorescens group, and is further contained in the clade composed of Pseudomonas gesardi, Pseudomonas ribanesis, Pseudomonas sinksansa, Pseudomonas muscillens, and Pseudomonas azotoformans. did. Among them, the homology with Pseudomonas azotoformans was particularly high.

3. Analysis of mycological properties 2. The colony morphology of the standard strains of Pseudomonas gesaldi, Pseudomonas ribanensis, Pseudomonas sinksansa, Pseudomonas mucillus and Pseudomonas azotoformans, which were considered to be related by the analysis result of the sequence of 16S rRNA gene of E. coli, was compared.
As a result, the W-14-1 strain was in good morphological characteristics with Pseudomonas azotoformans or Pseudomonas ribanensis in particular, suggesting that it is one of the species.
In order to determine the species of the W-14-1 strain, more detailed mycological properties of the W-14-1 strain, Pseudomonas azotoformans and Pseudomonas ribanesis were examined and compared according to a predetermined method described below.

First, the bacteriological properties of the W-14-1 strain are as follows. Gram-negative bacilli do not form spores, the total length of the cells is 1.5 to 2.5 μm, the total width is about 0.8 μm, and motility is recognized. It shows rough irregular colonies on standard agar and produces fluorescent dye on King B medium. No growth was observed at 41° C., catalase activity was positive, OF medium test was negative for oxidation, nitrate reduction was negative, indole production was negative, urease activity was negative, gelatin degradation was positive, β-galactosidase activity was negative, β-glucosidase activity is negative. In the LOPAT test, levan production was positive, potato tuber rot was negative, tobacco hypersensitivity reaction was negative, oxidase activity was positive, and arginine degradation was positive. Starch assimilability is negative, and as for assimilation of carbon compounds such as sugars and organic acids, D-glucose is positive, L-arabinose is positive, D-mannose is positive, D-mannitol is positive, N- Acetyl-D-glucosamine is positive, maltose is negative, potassium gluconate is positive, n-capric acid is positive, adipic acid is negative, DL-malic acid is positive, sodium citrate is positive, phenyl acetate is negative, sucrose Is positive, trehalose is positive, ribitol (adnit) is positive, sorbitol is positive, butyric acid is negative, propionic acid is positive, and propylene glycol is negative. The results are shown in Table 1.

Among the items in Table 1, reduction of nitrate, indole production, OF medium test, arginine degradation, urease activity, gelatin degradation, β-galactosidase activity, β-glucosidase activity, D-glucose assimilability, L- Utilization of arabinose, utilization of D-mannose, utilization of D-mannitol, utilization of N-acetyl-D-glucosamine, utilization of maltose, utilization of potassium gluconate, n- For the assimilability of capric acid, assimilability of adipic acid, assimilation of DL-malic acid, assimilation of sodium citrate, and assimilation of phenyl acetate, API20NE (manufactured by Nippon Biomerieux Co., Ltd.) was used. Then, the test was conducted according to the attached protocol. Oxidase Reagent (manufactured by Japan Biomerieux Co., Ltd.) was used for oxidase activity, and ID color Catalase (manufactured by Japan Biomerieu Co., Ltd.) was used for catalase activity. As for the King B medium used in the King B medium test, King B medium manufactured by Eiken Chemical Co., Ltd. was used. The Gram stain was performed using Color Gram 2 (manufactured by Nippon Biomerieux Co., Ltd.). Regarding the Levan production test, the strain was streaked onto a plate of normal agar medium supplemented with 5% sucrose and cultured at 25°C for 3 days to form white viscous, large dome-shaped colonies. Was judged to be positive.

The potato tuber decay test, tobacco hypersensitivity test, starch assimilation test, and assimilation of carbon compounds such as sugars and organic acids can be measured by known methods. For example, the method described in Patent Document 1 can be used.

In order to clarify whether the W-14-1 strain is Pseudomonas azotoformans or Pseudomonas ribanesis, the known standard strains of Pseudomonas azotoformans NBRC12693 strain and Pseudomonas ribanesis CIP105460 strain are shown in Table 1. The item was tested. The results are shown in Table 1.

Further, Pseudomonas fluorescens, which is a closely related species of both species, is known to exert pathogenicity on tomato and cause tomato stem nematode bacterial disease. The following tests were carried out in order to investigate whether such pathogenicity is exerted on tomato.
The W-14-1 strain was cultured in a liquid medium until A600 (absorbance at a wavelength of 600 nm) reached 1.0, and the culture solution was spray-inoculated on the main stem and petiole centered around the petiole base of tomato seedlings. did. After cultivating for 7 days, the seedlings were observed and no symptom of tomato stem nematode bacterial disease was found. The seedlings were also observed after cultivating for 14 days and after cultivating for 21 days. Similarly, no symptom of tomato stem nematode bacterial disease was observed. In addition, after injuring the tomato seedlings with the above-mentioned culture solution of the W-14-1 strain, the portion was inoculated and the same test was carried out. No sign was seen. Further, the above culture solution of the W-14-1 strain was injected and inoculated into the seedlings of tomato with a syringe, and the same test was carried out. It was

From the results in Table 1, the W-14-1 strain differs from the Pseudomonas ribanesis CIP105460 strain in that the morphological characteristics of the side surface, the peripheral edge, and the surface of the colony, the reducing ability of nitrate, the degrading ability of gelatin, and the degrading ability of alginic acid. The presence or absence of sucrose assimilation, propionic acid assimilation, and propylene glycol assimilation.
On the other hand, the W-14-1 strain differs from the Pseudomonas azotoformans NBRC12693 strain only in the nitrate reducing ability, the gelatin degrading ability, the adipic acid assimilating ability, and the propylene glycol assimilating ability. It was
From the above results, since the W-14-1 strain has higher homology to Pseudomonas azotoformans than Pseudomonas ribanesis, the W-14-1 strain was identified as Pseudomonas azotoformans.

4. Preparation of Cell Suspension A 300 ml Erlenmeyer flask was charged with 150 ml of a standard liquid medium (0.25% yeast extract, 0.5% peptone, 0.1% glucose, pH 7.0) and sterilized by heating. Pseudomonas azotoformans strain W-14-1 (NITE BP-02371) was pre-inoculated with 0.1 ml of a preculture, and cultured in a reciprocating shaker at 30° C. and 100 rpm for 3 days. The obtained culture solution was centrifuged to remove the supernatant, and the precipitate was washed with water, and again centrifuged to remove the supernatant and the precipitate was washed with water. In this way, a bacterial cell suspension of Pseudomonas azotoformans strain W-14-1 (hereinafter referred to as "bacterial cell suspension of strain W-14-1") was prepared.

5. Production of a plant disease controlling and hydrating composition A microbial cell suspension of the W-14-1 strain was freeze-dried. The viable cell count of the dried product was 1.0×10 ¹¹ cfu/g. A wettable powder composition (hereinafter referred to as "W-14-1 strain") was prepared by uniformly mixing 10 parts by weight of the microbial cell dried product, 9 parts by weight of polyoxyethylene allylphenyl ether sulfate ammonium, and 81 parts by weight of calcium sulfate dihydrate. Wet powder"). The wettable powder composition is a drug containing Pseudomonas azotoformans strain W-14-1 as an active ingredient.

[Test Example 3] Lettuce rot (bacterial disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment solution was spray-treated on lettuce planted in the field. The spraying treatment was performed three times at intervals of one week. On the day of the first spraying treatment, a pathogenic bacterium of lettuce rot (Pseudomonas cichorii) was spray-inoculated with the aqueous suspension (A600=0.001). One week after the third application, the degree of disease onset was investigated according to the following criteria, and the control value was calculated based on the following equations 5 and 6. As a result, the control value was 65.0.

Criterion 0: No disease occurrence Criterion 1: Criteria in which a part of the outer leaves are affected 2: Criteria in which a part of the outer leaves and part of the head leaves are found Criterion 3: Most of the head leaves are affected or more damage Is recognized

(Equation 5)
Disease severity=((1×n1+2×n2+3×n3)/(3×total number of surveyed strains))×100
n1 to n3 are the number of shares corresponding to each of the criteria 1 to 3 (formula 6)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

[Test Example 4] Citrus canker disease (bacterial disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment solution was sprayed on the entire Navel tree. The spraying treatment was carried out at the initial stage of leaf spreading and twice every two weeks thereafter, for a total of three times. Nine days after the final spraying, the degree of disease of citrus canker disease (pathogen Xanthomonas campestris pv. citri) on navel leaves was investigated based on the following criteria, and the disease severity was calculated based on the following equations 7 and 8. And the control value was calculated. As a result, the control value was 62.5.

Criteria 0: No lesions Criteria 1: Number of lesions is 1 to 3 Standard 3: Number of lesions is 4 to 10 Standard 5: Number of lesions is 11 to 20 Standard 7: Number of lesions is 21 or more

(Equation 7)
Disease severity=((1×n1+3×n3+5×n5+7×n7)/(7×total number of investigated leaves))×100
n1, n3, n5, and n7 are the number of leaves corresponding to each of the criteria 1, 3, 5, and 7 (Equation 8)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

[Test Example 5] Lettuce spot spot bacterial disease (bacterial disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The spraying treatment was performed 4 times at 1-week intervals. On the day of the first spraying treatment, an aqueous suspension (A600=0.001) was spray-inoculated with a pathogenic bacterium of Xanthomonas campestris pv. One week after the fourth application, the degree of disease onset was investigated based on the following criteria, and the disease degree and control value were calculated based on the following equations 9 and 10. As a result, the control value was 87.9.

Criterion 0: No disease occurrence Criteria 1: Disease occurrence on a part of the outer leaves Criteria 2: Most of the outer leaves are affected Criteria 3: Outer leaves and inner leaves are affected Criteria 4: Heading leaves are affected

(Equation 9)
Disease severity=((1×n1+2×n2+3×n3+4×n4)/(4×total number of surveyed strains))×100
n1 to n4 are the number of shares corresponding to each of the criteria 1 to 4 (equation 10)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

[Test Example 6] Tomato gray mold disease (filamentous fungus disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment liquid was applied to tomatoes that had been planted in the field. The spraying treatment was performed 4 times at 1-week intervals. On the day of the first spraying treatment, a spore-forming PDA plate of the pathogenic bacterium of Botrytis cinerea (Tomato Botrytis cinerea) was placed in the field. Five investigations were carried out, including the final investigation 6 days after the fourth application, to investigate the presence or absence of disease in all young fruits. In addition, at the time of the final survey, the total number of fruit set was counted, and the disease incidence and control value were calculated based on the following formulas 11 and 12. As a result, the control value was 89.9.

(Equation 11)
Diseased fruit rate = (number of diseased fruits / total number of surveyed fruits) x 100
(Equation 12)
Control value=(1-(fruit-caused fruit rate of treated area/fruit-caused fruit rate of untreated area))×100

[Test Example 7] Pear black spot disease (filamentous fungus disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment liquid was applied to the whole pear tree planted in the field. The spraying treatment was performed twice, at the beginning of flowering and immediately after the fall of flowers. Two days after the second application, the presence or absence of pear black spot disease (pathogenic fungus Alternaria kikuchiana) on pear leaves was investigated and the diseased leaf rate and control value were calculated based on the following equations 13 and 14. did. As a result, the control value was 60.5.

(Equation 13)
Diseased leaf rate=(number of diseased leaves/total number of investigated leaves)×100
(Equation 14)
Control value=(1-(diseased leaf rate of treated plot/diseased leaf rate of untreated plot))×100

[Test Example 8] Citrus gray mold disease (filamentous fungus disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment liquid was applied to the entire citrus tree planted in the field. The spraying treatment was carried out twice, at the early stage of leaf spreading and at the early stage of flowering. In addition, the causative agent of Botrytis cinerea, a causal agent of citrus gray mold (Botrytis cinerea), was arranged in the field with pumpkin fruits that had their spores formed at the 7-minute flowering stage. The presence or absence of the disease on citrus flowers was investigated during the valve drop period, and the diseased flower rate and the control value were calculated based on the following formulas 15 and 16. As a result, the control value was 70.3.

(Equation 15)
Diseased flower rate = (number of diseased flowers / total number of surveyed flowers) x 100
(Equation 16)
Control value=(1-(diseased flower rate of treated area/diseased flower rate of untreated area))×100

[Test Example 9] Citrus scab disease (filamentous fungus disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment liquid was applied to the entire citrus tree planted in the field. The spraying treatment was carried out three times in the early stage of leaf spreading, the early stage of flowering and the period of valve drop. Thirteen days after the third application, the degree of onset of citrus scab (pathogenic fungus Elsinoe fawcettii) on citrus leaves was investigated according to the following criteria, and the degree of onset and the degree of onset were calculated based on the following equations 17 and The control value was calculated. As a result, the control value was 68.8.

Standard 0: No lesions Standard 1: Number of lesions is 1 to 5 Standard 3: Number of lesions is 6 to 20 Standard 5: Number of lesions is 21 to 50 Standard 7: Number of lesions is 51 or more

(Equation 17)
Disease severity=((1×n1+3×n3+5×n5+7×n7)/(7×total number of investigated leaves))×100
n1 to n7 are the number of leaves corresponding to each of the criteria 1 to 7 (Equation 18)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

[Test Example 10] Potato summer blight (filamentous fungal disease) control effect test (field test)
A wettable powder of the W-14-1 strain was diluted 1000 times with water to prepare a treatment liquid. The treatment liquid was applied to potatoes planted in the field. It was carried out twice at 10-day intervals. Four days after the second spraying, the extent of the occurrence of potato summer blight (pathogenic fungus Alternaria solani) on potato leaves was investigated based on the following criteria, and the disease severity and control value were calculated based on the following equations 19 and 20. Was calculated. As a result, the control value was 52.2.

Criterion 0: No lesions on leaves Criteria 1: Lesion area is less than 5% of leaf area Criterion 2: Lesion area is 5 to less than 25% of leaf area Criterion 3: Lesion area is 25 to less than leaf area Less than 50% Standard 4: Lesion area is 50% or more of leaf area

(Formula 19)
Disease severity=((1×n1+2×n2+3×n3+4×n4)/(4×total number of surveyed strains))×100
n1 to n4 are the number of shares corresponding to each of the criteria 1 to 4 (equation 20)
Control value=(1-(risk rate of treated area/risk degree of untreated area))×100

As described above, the present microorganism is effective as a bacterial disease, at least, lettuce soft rot, Chinese cabbage soft rot, lettuce rot, citrus canker, lettuce spot bacterial disease, and as a filamentous fungal disease, at least gray mold of tomato. , Pear black spot, citrus gray mold, citrus scab and potato summer plague. Thus, it was revealed that both bacterial diseases and filamentous fungal diseases have a high control effect.
Further, if there is a controlling effect on both bacterial disease and filamentous fungus disease in this way, in potato, soft rot (bacteria) and summer sickness (filamentous fungus), in citrus, canker (bacteria) and gray mold (filamentous fungus). Fungus), or canker (bacteria) and scab (filamentous fungus), lettuce, soft rot (bacteria) and gray mold (filamentous fungus), rot (bacteria) and gray mold (filamentous fungus), Alternatively, it has been found that it is possible to simultaneously exert an effect against the spotted bacterial disease (bacteria) and the gray mold disease (filamentous fungus) only by this microorganism.

Claims (4)

Pseudomonas Azotoformans W-14-1 strain NITE BP-0237 1. A method for controlling plant diseases, which comprises treating Pseudomonas azotoformans W-14-1 strain NITE BP-02371 with plants and/or cultivated soil of the plants. Control agent of plant diseases which contains the Pseudomonas azo preparative folder performance W-14-1 strain NITE BP-0237 1及 beauty surfactant. Pseudomonas azotoformans W-14-1 strain NITE BP-02371 1 is 1 part by mass, and the compounding ratio of the surfactant is 0.01 to 30 parts by mass. Control agent.