JP6826346B2 - Anti-allergic agents, as well as foods, cosmetics, and medicines containing them - Google Patents

Description

The present invention relates to novel antiallergic agents and foods, cosmetics, and pharmaceuticals containing them.

Bamboo has long been used for daily necessities and other daily necessities, as well as for preventing the collapse of slopes and for reinforcing embankments.
However, in recent years, the amount of bamboo used in Japan has been declining due to the fact that household materials have been replaced by chemical products, embankments have been replaced by secondary products such as concrete blocks, and bamboo shoots are now mostly imported.
In addition to the low utility value of bamboo, devastated bamboo grove is scattered all over Japan due to the aging of bamboo grove managers and bamboo craftsmen.
In addition, the devastation of bamboo grove poses a risk of disasters such as landslides, which has become a social problem.

Under these circumstances, bamboo charcoal is produced from healthy bamboo in the 4th to 7th grades for the purpose of effective utilization of bamboo. Bamboo charcoal is mainly used for the purpose of deodorizing or dehumidifying.
In addition, a bamboo vinegar solution obtained by cooling smoke generated during the production of bamboo charcoal is produced. Bamboo vinegar is mainly used for deodorizing, sterilizing, antibacterial, or insect repellent purposes.

On the other hand, an anti-allergic composition containing a distilled solution of bamboo vinegar as an active ingredient has been proposed (Patent Document 1).
Antihistamines and steroids are used to improve allergic symptoms, but depending on the person or the symptoms, sufficient effects may not be obtained, or conversely, drug-induced side effects may occur. Therefore, attempts are being made to develop antiallergic agents using natural products.
In the example of Patent Document 1, a coarse bamboo vinegar obtained by cutting 5th to 6th grade Moso bamboo, subjecting it to smoke treatment, and then collecting the smoke emitted when the bamboo is carbonized is further added. It is stated that no harmful substances such as carcinogens were detected in the bamboo vinegar distilled solution obtained by distillation purification. It is also described that this bamboo vinegar distilled solution had an inhibitory effect on allergies.

Further, an antiallergic agent containing a bamboo extract as an active ingredient has been proposed (Patent Document 2).
In the examples of Patent Document 2, the bamboo extract obtained by ether extraction of the crushed Moso bamboo stalk and the bamboo extract obtained by the methanol extraction of the crushed bee leaf stalk under heating and reflux are used. It is stated that it had allergic activity.
Further, Patent Document 2 describes that the site where bamboo is used is leaves, stems, roots, etc., but the epidermis of the stem is most suitable because it has high antiallergic activity.

Furthermore, an external preparation for skin characterized by containing a bamboo extract has been proposed (Patent Document 3).
In the example of Patent Document 3, the water extract of bamboo obtained by hot water extraction of the crushed product of Moso bamboo under heating and reflux conditions releases histamine that causes allergic symptoms in an experiment using cultured cells. It is stated that it was suppressed. In addition, it is described that the secretion of leukotriene was suppressed by the experiment using the same cells.
Further, Patent Document 3 describes that the crushed bamboo product is powdered in consideration of the extraction operation.

Japanese Unexamined Patent Publication No. 2005-179245 Japanese Unexamined Patent Publication No. 9-278662 Japanese Unexamined Patent Publication No. 2003-212786

Bamboo charcoal is a material that competes with charcoal such as Bincho charcoal. Bamboo charcoal is said to be excellent as an adsorbent for deodorizing and dehumidifying because it has smaller pores and a larger surface area than charcoal.
However, since the production of bamboo charcoal requires equipment such as a bamboo kiln, it is difficult to start a new business.
In addition, due to the structure of bamboo, it is difficult to control the water content when carbonizing, it takes time and effort, and as a result of the low coal collection rate, it becomes more expensive than charcoal.
Furthermore, bamboo charcoal is considered unsuitable as a fuel used for charcoal grilling and the like because it easily disappears.
Due to these circumstances, the consumption of bamboo charcoal is far below that of charcoal.
Therefore, it is considered difficult to reduce the degraded bamboo grove by promoting the use of bamboo in the production of bamboo charcoal.

There is a possibility that the use of bamboo can be promoted by producing the bamboo vinegar distilled solution used for a specific purpose described in Patent Document 1.
However, even if harmful substances such as carcinogenic substances can be reduced by the smoking step and the distillation purification step described in Patent Document 1, strict quality control is required for the obtained bamboo vinegar distilled liquid. ..
In addition, special care must be taken when producing a bamboo vinegar distillate to be used by humans, and capital investment and the like are costly.
Therefore, it is considered difficult to reduce the devastated bamboo grove by promoting the use of bamboo in the production of the bamboo vinegar distilled solution described in Patent Document 1.

There is a possibility that the utilization of bamboo can be promoted by producing an organic solvent extract of crushed bamboo stems or leaves described in Patent Document 2.
However, in order to process a large amount of bamboo, it takes time and effort to cut the bamboo into appropriate sizes, and at the same time, an extraction operation must be performed, which requires crushing equipment and extraction equipment. In addition, since extraction is performed with an organic solvent, equipment for distilling off the organic solvent is required.
Moreover, given that the epidermis of bamboo stalks is suitable, the amount of bamboo material required to obtain a certain amount of extract is not very high.
Therefore, it is considered difficult to improve the devastation of bamboo grove by promoting the use of bamboo in the production of organic solvent extracts of bamboo.

There is a possibility that the use of bamboo can be promoted by producing a hot water extract of a crushed bamboo product described in Patent Document 3.
However, the pulverized product is powdered in consideration of the extraction operation. In addition, the extraction step is performed under heating recirculation. Therefore, in order to obtain an extract of a certain amount or more, a crushing facility and an extraction facility are required.
Also, the amount of bamboo required to obtain a certain amount of extract is not very large.
Therefore, it is considered difficult to improve the devastation of bamboo grove by promoting the use of bamboo in the production of hot water extracts of crushed bamboo.

The present invention has been made in view of such circumstances, and an object of the present invention is to provide a novel bamboo utilization technique that leads to a reduction in degraded bamboo grove.

The present inventors have conducted diligent studies for the purpose of providing a novel bamboo utilization technology that leads to a reduction in degraded bamboo grove.
As a result, the present inventors have found that bamboo water obtained relatively easily by cutting a culm of young bamboo in a specific growing period at an appropriate position has an anti-allergic effect.
The present invention has been completed based on such findings, and includes the following configurations.

(Structure 1)
An antiallergic agent characterized by containing bamboo water.

(Structure 2)
An antiallergic food containing the antiallergic agent according to the first composition.

(Structure 3)
An anti-allergic cosmetic containing the anti-allergic agent according to the first composition.

(Structure 4)
An anti-allergic drug, which comprises the anti-allergic agent according to the first composition.

According to the present invention, a novel bamboo utilization technique is provided.

Hereinafter, embodiments of the present invention will be described exemplary. Any person having ordinary knowledge in the art can modify or improve the present invention within the technical idea of the present invention. In addition, any simple modification or modification of the present invention belongs to the scope of the present invention. Therefore, the embodiments described below are not intended to limit the scope of the present invention.

(Bamboo water collection)
Bamboo water used in the present invention is a liquid sucked up through a rhizome by young bamboo in a specific growing season.
Bamboo water may be stored in the hollow between bamboo nodes after being sucked up from the rhizome.
In addition, bamboo water may seep out from between the culm of young bamboo (corresponding to the stem or trunk of a normal plant) and the culm of young bamboo (corresponding to the skin of bamboo shoots) after being sucked up from the rhizome.

The bamboo water used in the present invention can be collected from bamboo belonging to the subfamily Bambusoideae of the Gramineae family. Examples of such bamboo include moso bamboo (Moso bamboo), madake (matake), and bee (light bamboo).

Bamboo water is collected from young bamboo in a specific growing season. The specific growth period is the period when young bamboo grows from bamboo shoots and grows to a height of 3.0 m to 5.0 m in the spring season before the rainy season in Japan from April to May. This is because a certain amount of bamboo water can be obtained by using the young bamboo that is in the growing season.

The bamboo water used in the present invention can be obtained by cutting the culm of the young bamboo in the specific growing season and collecting the liquid naturally accumulated in the hollow, but when securing a certain amount or more of the bamboo water. The following two methods can be adopted for.

Wakatake culms with a height of 3.0 to 5.0 m are cut at a position 1.0 to 2.0 m below the tip in the evening, and then left for about 1 to 3 weeks. The cut-off tip is a delicacy ingredient that can be eaten as a "bamboo shoot". As a result, the bamboo water between the nodes can be recovered in a short period of time.
After about 1 to 3 weeks, shake the bamboo cut from the culm from the feet to identify the hollow between the nodes that make the sound of water. Then, by cutting the upper part of the hollow, the bamboo water stored in the hollow can be collected.
By cutting the tip of the growing part of young bamboo, the liquid sucked up from the rhizome loses its place without being supplied to the growing part after passing through the bamboo culm, and is stored in the air. I used it.

In order to directly obtain a larger amount of bamboo water, the following method can be adopted.
Cut a young bamboo culm with a height of about 3.0 to 5.0 m in the evening at a position 1.0 to 2.0 m above the ground and about 2 to 3 cm above the node.
Preferably, it is cut closer to 1.0 m from the ground. This is because more bamboo water can be recovered.
Next, the vinyl mouth for collecting bamboo water is tied so as to cover the entire cut surface. At this time, firmly fix it so that water does not leak from the tied part. Then, the next morning, the bamboo water collected in the plastic bag is collected.
This utilizes the fact that the liquid sucked up from the rhizome overflows from the cut surface after passing through the bamboo culm.
By this method, about 0.5 to 2.0 liters of bamboo water can be collected from the evening of the previous day to the next morning.

In one embodiment, the present invention relates to a method for preparing bamboo water, which is useful for producing antiallergic agents described in detail below.
Such a method is a step of (a) cutting a young bamboo culm grown from a bamboo shoot to a height of 3.0 to 5.0 m at a position of 1.0 to 2.0 m from the ground, and (b) of the bamboo. A method for preparing bamboo shoots, which comprises a step of recovering a liquid exuding from the cut surface after being absorbed from the rhizome, and (c) a step of removing foreign matter from the recovered liquid.
In a preferred embodiment, the cutting in the step (a) is a step of cutting at a position 1.0 to 2.0 m above the ground and 2 to 3 cm above the bamboo knot.

(Anti-allergic agent)
The antiallergic agent of the present invention is characterized by containing bamboo water.
The anti-allergic agent of the present invention has an anti-allergic action as described in Examples described later.
Therefore, the antiallergic agent of the present invention is used to improve allergic symptoms such as pollinosis, allergic rhinitis, atopic dermatitis, other allergic dermatitis, allergic conjunctivitis, anaphylactic reaction, and bronchial asthma. ..
In the present invention, "improvement" of allergic symptoms includes all aspects of reducing or suppressing allergic diseases, symptoms or conditions caused by allergic reactions, for example, prevention, suppression, alleviation, aggravation. These include delays, arrests, and treatments.

The antiallergic agent of the present invention is used as a preparation obtained by subjecting bamboo water collected as described above to at least one or more preparation steps, or bamboo water or its preparation, and foods and cosmetics. Alternatively, it can be provided as a composition in combination with other materials or additives used in products such as pharmaceuticals.
Bamboo water preparations can be provided in various forms such as liquid, semi-solid and solid.

The liquid bamboo water preparation can be prepared by removing foreign substances (for example, chips and the like) mixed in the bamboo water. Foreign matter is removed from the collected bamboo water stock solution, a concentrated solution thereof, a diluted solution, or the like.
As a method for removing foreign matter, a method known to those skilled in the art can be adopted. Examples of such a method include filtration, centrifugation and the like.

Bamboo water preparations can be prepared as bamboo water concentrates.
As the concentration method, a method known to those skilled in the art can be adopted. Examples of such a method include vacuum concentration, reverse osmosis membrane method and the like.

Bamboo water preparations can be prepared as a diluted solution of bamboo water.
The solvent used for dilution can be appropriately selected according to the product to which the antiallergic agent of the present invention is applied. Examples of such a solvent include, when the product is a lotion, purified water alone, or an aqueous solution containing glycerin, a surfactant, ethanol and the like used in the lotion. It is also possible to prepare a diluted bamboo water by adding other raw materials to the stock solution of bamboo water or bamboo water diluted with water.

Semi-solid bamboo water preparations can be prepared by increasing the concentration rate. For example, a paste-like preparation can be obtained by appropriately setting the conditions for concentration under reduced pressure.

The solid bamboo water preparation can be prepared by drying the bamboo water or its preparation.
As a drying method, a method known to those skilled in the art can be adopted. Examples of such a method include freeze-drying, spray drying, fluidized bed drying, vacuum drying and the like. After adding an excipient such as dextrin and mixing, spray drying can also be performed.
Further, after drying, the particle size can be adjusted by pulverizing or granulating by a method known to those skilled in the art.

In any step of producing the bamboo water preparation, a step of sterilizing or sterilizing the bamboo water can be provided.
As a sterilization or sterilization method, a method known to those skilled in the art can be adopted. Examples of such a method include heat sterilization and filtration sterilization. When the bamboo water or its preparation is dried by heating, the sterilization step can also be performed by appropriately setting the conditions.
In addition, a sterilization or sterilization step can be performed at the stage of a composition or a final product in which bamboo water or a preparation thereof is combined with other materials or the like.

As described above, the antiallergic agent of the present invention can be in any form, as it is, or as a composition obtained by combining bamboo water or a preparation thereof with other materials described below, if necessary. , Can be molded into various shapes and provided.
The antiallergic agent of the present invention is used as a supplement, an additive for foods used for a specific purpose, a raw material for cosmetics, a pharmaceutical product, or a raw material thereof.
Further, the antiallergic agent of the present invention can be used as it is as a product such as an additive for foods, an additive for cosmetics, and an additive for pharmaceuticals.

The present inventors have found that bamboo water has an anti-allergic effect, and have completed the present invention.
As shown in Examples described later, it has been confirmed in animal experiments (passive anaphylactic reaction induction test) that the bamboo water preparation obtained without performing the extraction step or the separation step has an effect of improving allergic symptoms. , Its usefulness is extremely high.
Wakatake has enough vitality to grow more than 1 m a day in spring from April to May. The present inventors have confirmed that the anti-allergic action of bamboo water is due to a component passing through a dialysis membrane having a molecular weight of 3000 cuts, but whether this action is due to a single component or a synergistic effect of a plurality of components. It is not yet clear whether it depends on the action.
However, it is considered that this high anti-allergic activity is closely related to the components in Wakatake, which have high vitality and are contained in a specific growth period, or the component groups or their composition ratios.

In addition, the bamboo water used in the antiallergic agent of the present invention has been ingested by humans for a long time.
Therefore, unlike bamboo vinegar, its derivatives, or bamboo extracts, the antiallergic agent of the present invention is highly safe and can be applied to various products with peace of mind.

Bamboo water, which is characteristically contained in the antiallergic agent of the present invention, is said to cut an appropriate position of native young bamboo at a specific time without involving a crushing step, an extraction step, the use of an organic solvent, etc. of the bamboo material. It can be obtained by a simple method.
Therefore, no special equipment is required when producing bamboo vinegar or bamboo extract. In addition, elderly people can easily collect bamboo water without much effort.
Therefore, the use of bamboo water for anti-allergic purposes is extremely cost-effective.

Bamboo water is obtained by cutting an appropriate position of young bamboo in a specific growing season as described above.
Bamboo itself does not undergo secondary hypertrophic growth, so if it is cut at an appropriate position during a specific growth period, the bamboo will die as it is.
Therefore, by utilizing the novel bamboo utilization technology according to the present invention, it is possible to quickly prevent further devastation of bamboo grove in mountainous areas by a labor-saving method, especially in the situation where the work is often performed by elderly people. .. In addition, cutting at a certain height or higher is highly safe in terms of bamboo grove management because no stump remains under the feet.

As described above, the present invention provides a novel technique for utilizing bamboo. This utilization technique is an antiallergic agent characterized by containing bamboo water obtained from young bamboo in a specific growing season by a relatively simple method.
This anti-allergic agent can safely improve allergic symptoms and can be expected to be highly cost-effective for its production. In addition, the implementation of the present invention in combination with the method of collecting bamboo water brings about the improvement of the devastated bamboo grove and is extremely effective in the prevention of disasters caused by the abandoned bamboo grove.

(Food for anti-allergy)
The antiallergic food of the present invention is characterized by containing the antiallergic agent of the present invention.
In addition, the food of the present invention is used to improve allergic symptoms, like the antiallergic agent of the present invention.
The food product of the present invention can be produced by a method known to those skilled in the art for producing food products in combination with the antiallergic agent of the present invention and other food materials, additives and the like.

The food of the present invention can be in any form that can be orally ingested, such as solutions, suspensions, emulsions, powders, solid moldings and the like.
The food of the present invention includes, for example, beverages such as tea, tea, coffee, frozen desserts, alcoholic beverages, carbonated beverages, dairy beverages, fruit juice beverages, nutritional drinks, and confectioneries such as candy, gummy, gum, chocolate, cookies, and biscuits. , Frozen desserts such as ice cream, dairy products such as yogurt and processed milk, flour products such as bread and cake mix, noodles such as buckwheat, processed fats and oils such as mayonnaise, whipped cream and dressing, processed marine products, processed livestock products, It can be manufactured as a processed agricultural product. The food product of the present invention can be produced by adding and containing the antiallergic agent of the present invention at the time of producing these food products.
In addition to other food materials, the food of the present invention includes sweeteners, colorants, preservatives, thickeners, stabilizers, gelling or pastes, antioxidants such as ascorbic acid, and coloring, if necessary. Agents, bleaching agents, fungicides or antibacterial agents, yeast foods, gum bases, citrus fruits, bitterness agents, enzymes, brighteners, flavors, acidulants, chewing gum softeners, seasonings, coagulants for tofu, emulsifiers, pH adjusters , Additives such as swelling agents, vitamins, minerals, nutritional enhancers such as amino acids, and manufacturing agents can be added.
It is also possible to combine known antiallergic substances such as glycyrrhizic acid, which are already used in foods.

Further, the food of the present invention can be produced as a health functional food (specified health functional food, functional food with functional claims, nutritionally functional food), so-called health food, concentrated nutritional supplement, liquid food, infant / infant food.

Under the system of each country, the food product of the present invention can be provided as a food product having an application for improving allergic symptoms and a function thereof.
For example, when targeting allergic conjunctivitis or allergic rhinitis, the food product of the present invention can be provided as a food product labeled as "reducing discomfort in the eyes and nose due to house dust, dust, etc." Even when allergic dermatitis is targeted, it is possible to display according to this.

In the food of the present invention, the content of the antiallergic agent of the present invention can be appropriately set according to the form of the food, the age of the person ingesting it, the purpose of ingestion, and the like.

Since the food product of the present invention is characterized by containing the antiallergic agent of the present invention, it shares its advantages and is extremely useful.

(Anti-allergic cosmetics)
The antiallergic cosmetic of the present invention is characterized by containing the antiallergic agent of the present invention.
The cosmetics of the present invention, like the antiallergic agents of the present invention, are used to improve allergic symptoms.
The cosmetic of the present invention can be produced by a method for producing a cosmetic known to those skilled in the art by combining the antiallergic agent of the present invention with other raw materials and additives usually used for producing the cosmetic.

The form of the cosmetic of the present invention is arbitrary, and includes, for example, skin care cosmetics such as lotion, milky lotion, cream, beauty liquid, pack, sunscreen, soap, face wash, foundation, concealer, makeup base, etc. Examples include make-up cosmetics such as lipstick, cheek red, eye shadow and eye liner, hair cosmetics such as shampoo and rinse, scalp cosmetics such as hair tonic, and aromatic cosmetics such as perfume.

Other raw materials and additives used in the production of the cosmetics of the present invention include, for example, oil-based raw materials such as hydrocarbons, vegetable oils, animal oils, higher alcohols, higher fatty acid esters, polyhydric alcohols, surfactants, and moisturizers. Polymer substances classified into agents, water-soluble polymers, biopolymers, film-forming polymers, powder polymers, inorganic polymers, etc., thickeners, preservatives, bactericides, ultraviolet absorbers, antioxidants Agents, coloring materials, fragrances, pH adjusters, ethanol, water, chelating agents, whitening agents, hair growth agents, rough skin inhibitors, wrinkle inhibitors, acne agents, blemishes / itching agents, axillary odor inhibitors, and astringent agents Examples thereof include physiologically active ingredients such as, vitamins, and amino acids.
It is also possible to combine known antiallergic substances such as glycyrrhizic acid, which are already widely used in cosmetics.

In the cosmetic of the present invention, the blending amount of the antiallergic agent can be appropriately set according to the form of the cosmetic and the age of the human being used. The blending amount can be set, for example, using the concentration of the solid content of bamboo water as an index.
In one embodiment, the cosmetics of the present invention can be produced as follows.
After filtering the bamboo water obtained as described above with sterilized gauze, the bamboo water preparation (900 ml) obtained by further filtering with a sterilized filter (pore size: 0.22 μm) is mixed with glycerin (50 ml) and antiseptic. The cosmetic water of the present invention can be produced by adding paraben (1.5 g) as an agent, further adjusting the total volume to 1000 ml with purified water, and then mixing the mixture.

Since the cosmetic of the present invention is characterized by containing the antiallergic agent of the present invention, it shares its advantages and is extremely useful.

(Anti-allergic drug)
The antiallergic drug of the present invention is characterized by containing the antiallergic agent of the present invention. The pharmaceutical products of the present invention include those classified as quasi-drugs under Japanese legal system.
The pharmaceutical product of the present invention, like the antiallergic agent of the present invention, is used to improve allergic symptoms.
The pharmaceutical product of the present invention is administered orally or parenterally based on the dosage form. As the parenteral administration, for example, transdermal absorption, subcutaneous injection, intravenous injection and the like are appropriately selected.
Oral administration is preferably adopted in that the characteristics of bamboo water, whose safety has been established by oral ingestion, can be utilized. Percutaneous administration is also preferably adopted in view of the common general knowledge that those taken orally can be safely applied as an external preparation.

When the pharmaceutical product of the present invention is an oral preparation, the antiallergic agent of the present invention is added as an active ingredient, and any of tablets, capsules, granules, powders, syrups, drops, pills, internal solutions, etc. It can be manufactured as a dosage form.
In this case, the pharmaceutical product of the present invention can be formulated by a method for producing a pharmaceutical product known to those skilled in the art by combining the antiallergic agent of the present invention with other additives usually used for producing an oral preparation.
Additives used in the production of the pharmaceutical product of the present invention include, for example, excipients, disintegrants, binders, lubricants, coating agents, dispersants, fluidizers, stabilizers, preservatives, buffers, and flavors. Examples thereof include agents, suspending agents, emulsifiers, flavoring agents, solubilizing agents, coloring agents, and thickening agents.
It is also possible to combine known antiallergic substances such as glycyrrhizic acid, which are already used as active ingredients of pharmaceutical products.

When the drug of the present invention is an oral preparation, the dose thereof can be appropriately set according to the age, body weight, sex, and symptoms of the person to whom the drug is applied, as long as allergic symptoms can be improved.
For example, the daily intake is preferably 1 to 2000 mg / kg, more preferably 2 to 2000 mg / kg, and even more preferably 5 as the weight of the solid content of bamboo water after removing foreign substances. It can be set at ~ 2000 mg / kg. The number of ingestions is arbitrary and can be appropriately set from 1 to several times a day.

When the pharmaceutical product of the present invention is an external preparation, the type of dosage form is not particularly limited, and the antiallergic agent of the present invention is added as an active ingredient, and an external solid preparation such as an external powder and an external application such as a lotion are used. It can be produced as a liquid agent, a spray agent such as an aerosol agent, an ointment agent, a cream agent, a gel agent, a patch agent such as a poultice agent, or the like.
In this case, the pharmaceutical product of the present invention can be formulated by a method for producing a pharmaceutical product known to those skilled in the art by combining the antiallergic agent of the present invention with other additives usually used for producing an external preparation.
Additives used in the production of the pharmaceutical product of the present invention include, for example, oil-in-water or water-in-oil emulsion base materials, mineral oil-based base materials such as petrolatum and paraffin, squalane, and lanolin. Examples thereof include animal and vegetable oil-based base materials, solubilizing agents, suspending agents, thickeners, emulsifiers, surfactants, stabilizers, preservatives, buffers, propellants and the like.
It is also possible to combine known antiallergic substances such as glycyrrhizic acid, which are already used as active ingredients of quasi-drugs for external preparations.

When the drug of the present invention is an external preparation, the amount of the antiallergic agent used of the present invention improves allergic symptoms according to the form of the external preparation and the age, weight, sex, symptoms, etc. of the person to whom the preparation is applied. As far as possible, the concentration of the solid content of the bamboo water can be used as an index and appropriately set.
The above-mentioned lotion of the present invention can also be used as a lotion.

Since the pharmaceutical product of the present invention is characterized by containing the antiallergic agent of the present invention, it shares its advantages and is extremely useful.

The anti-allergic agent characterized by containing bamboo water of the present invention, and foods, cosmetics, and pharmaceuticals characterized by containing the same are added because they are excellent in their safety and anti-allergic activity. High value.
Further, according to the present invention, since high value-added materials and raw materials for products can be obtained by a relatively simple method, great cost effectiveness can be expected.
Therefore, it can be expected that the use of the technique of the present invention will motivate the abandoned bamboo grove and new business entrants to manage the bamboo grove.
In addition, the collection of bamboo water, which is the raw material, naturally prevents the devastation of bamboo grove.
Therefore, the provision of the novel bamboo utilization technique of the present invention has an extremely large degree of social contribution.
In addition, to enhance the momentum of new anti-allergic agents and foods, cosmetics, and pharmaceuticals containing them, thereby contributing to industrial promotion including business expansion, establishment and employment creation in the middle mountain area with bamboo grove. There is expected.

(Preparation of bamboo water)
In the evening of April 2016, in a bamboo grove in Onashi-cho, Takehara City, Hiroshima Prefecture, a young bamboo culm of Moso bamboo, which was about 4 m tall, was cut at a position 3 cm above the node about 1 m from the ground. Next, the cut surface was covered with the mouth of the vinyl bag, and the bamboo water exuding from the cut surface was tied and fixed with a gum tape so that it could be collected in the vinyl bag.
The next morning, we were able to collect 1.5 liters of bamboo water per young bamboo in a plastic bag. Then, the collected bamboo water was filtered with sterilized gauze to obtain a stock solution of bamboo water (stock solution of bamboo water). After water sampling, it was promptly managed by a storage method by freezing to prevent deterioration.
Subsequently, the bamboo water stock solution that had been cryopreserved was freeze-dried to obtain a dry powder of bamboo water. The solid content concentration of the bamboo water stock solution calculated from the weight of the obtained dry powder of bamboo water was 5 mg / ml.
The dry powder of bamboo water thus obtained is dissolved in ultrapure water and further filtered and sterilized (pore size: 0.22 μm) to obtain two different concentrations (solid content concentration: 8.3 mg / 100 μl). The antiallergic agent of the present invention of Preparation Example 1) and 20 mg / 100 μl (Preparation Example 2)) was prepared and used in the following animal experiments.

(Passive anaphylactic reaction induction test)
100 μl of PBS (phosphate buffered saline), Preparation Example 1 or Preparation Example 2 was intraperitoneally administered to wild-type BALB / c mice (purchased from Hiroshima laboratory animals) consisting of 7 mice in each group.
Twenty-two hours later, 10 μl of anti-dinitrophenol (DNP) -IgE antibody adjusted to 150 ng / 10 μl was subcutaneously administered to the left ear of the mouse, and 10 μl of PBS was subcutaneously administered to the right ear as a negative control.
Twenty-two hours after subcutaneous administration, 100 μl of PBS, Preparation Example 1 and Preparation Example 2 were intraperitoneally administered to each group again, and 2 hours later, 200 μg of DNP-HSA (dinitrophenol binding) containing 1.5% Evans blue dye was administered. Human serum albumin) was intravenously administered at 100 μl from the tail vein.
Subsequently, 30 minutes after intravenous administration of DNP-HSA containing the pigment, the mice were euthanized, the pinna was excised, and the pinna was eluted with formamide overnight at 63 ° C. to elute into the pinna. Evans blue was extracted and the absorbance at 595 nm was measured. The amount of Evans blue leaked from the auricle was calculated from the calibration curve using a known amount of Evans blue, and the amount of leaked from the left ear to which the anti-DNP-IgE antibody was administered led to the leakage of the right ear to which PBS was administered, which is a negative subject. The value obtained by subtracting the amount was taken as the amount of auricular pigment leakage.
The small amount of pigment leakage indicates that inflammation and vascular hyperpermeability caused by an allergic reaction (passive anaphylactic shock) are suppressed via the antigens DNP and anti-DNP-IgE antibody. ..
The results are shown in Table 1.

The results in Table 1 show that the antiallergic agent of the present invention suppressed the symptoms caused by the allergic reaction in vivo.
In addition, in the group to which the low concentration of Preparation Example 1 was administered, the symptoms due to the anaphylactic reaction were clearly suppressed, and the antiallergic effect was obtained. Therefore, the antiallergic activity of bamboo water was very high. Understandable.
These results indicate that the antiallergic agent of the present invention, which is characterized by containing bamboo water obtained without performing an extraction or separation step, is extremely useful for the purpose of improving allergic symptoms.

Claims (4)

An antiallergic agent characterized by containing bamboo water obtained from Moso bamboo . An antiallergic food containing the antiallergic agent according to claim 1. An antiallergic cosmetic containing the antiallergic agent according to claim 1. An antiallergic drug comprising the antiallergic agent according to claim 1.