JP6836626B2 - Method to improve sterilization property of quaternary ammonium salt type surfactant - Google Patents

Description

The present invention relates to a wet sheet in which a base sheet is impregnated with a liquid agent.

Wet sheets containing disinfectants or alcohol are known. Wet sheets are used to wipe off dirt around kitchens, tables, furniture, electrical appliances, etc. In addition, the wet sheet is also used to wipe off dirt on the body such as around the hands, fingers and mouth. It is important that the skin is not irritating to wipe the area around the mouth, and it can be predicted that it will enter the body, so safety is especially important. Patent Documents 1 to 3 describe wet sheets impregnated with a liquid agent containing alcohol. Patent Document 4 describes a wet sheet impregnated with a liquid agent containing a disinfectant.

Japanese Unexamined Patent Publication No. 2004-16730 Japanese Unexamined Patent Publication No. 2005-296240 Japanese Unexamined Patent Publication No. 2011-30793 Japanese Unexamined Patent Publication No. 2018-27916

Since the wet sheets described in Patent Documents 1 to 3 contain alcohol, they have a high sterilizing effect. However, when used to wipe off dirt from the body, the skin may show an allergic reaction to alcohol, and it cannot be said that it is less irritating to the skin.

The wet sheet described in Patent Document 4 does not contain alcohol and contains a sterilizing agent, and therefore has a high sterilizing effect. However, since the impregnated disinfectant is alkaline, it may be irritating to the skin.

Therefore, an object of the present invention is to provide a wet sheet having a high antibacterial and antibacterial effect but low irritation to the skin.

The present invention is a wet sheet in which a base sheet made of a fiber material is impregnated with a liquid agent.
The liquid agent is
(A) Lactic acid, citric acid, malic acid, acetic acid or salts of these acids, or combinations of two or more selected from these acids and salts,
(B) Quaternary ammonium salt-type surfactant, and (C) water,
The above-mentioned problems are solved by providing a wet sheet containing, ethanol-free, and having a pH of 3.0 or more and less than 6.0 at 25 ° C.

According to the present invention, a wet sheet having both a high antibacterial and antibacterial effect and low irritation to the skin is provided.

Hereinafter, the present invention will be described based on its preferred embodiment. The wet sheet of the present invention is obtained by impregnating a base sheet made of a fiber material with a liquid agent.

From the viewpoint of imparting an antibacterial effect, the liquid preparation contains the component (A). Examples of (A) include lactic acid, citric acid, malic acid, acetic acid or salts of these acids, or a combination of two or more selected from these acids and salts. Of these, lactic acid or a salt thereof, which gives an image of food from the viewpoint of security and safety, is preferable. Among lactic acids, fermented lactic acid obtained by fermentation, which gives an image of food from the viewpoint of security and safety, is preferably used. Examples of the salt include alkali metal salts such as potassium and sodium.

The content of the component (A) in the liquid preparation is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, and preferably 1.0% by mass or less from the viewpoint of imparting high antibacterial properties. More preferably, it is 0.5% by mass or less. The content of the component (A) is preferably 0.1% by mass or more and 1.0% by mass or less, and more preferably 0.2% by mass or more and 0.5% by mass or less in the liquid preparation. is there. When the acid is used in the salt state, the content of the component (A) is an amount converted into an acid (acid equivalent amount). When two or more kinds are contained as the component (A), the content of the component (A) is the total amount.

From the viewpoint of imparting a sterilizing effect, the liquid preparation contains the component (B). Examples of (B) include a quaternary ammonium salt-type surfactant represented by the following general formula (1).

(In the formula, R ¹ is a linear or branched alkyl group having 6 to 28 carbon atoms, a linear or branched alkenyl group having 6 to 28 carbon atoms, or the alkyl group or the alkenyl group being an amide. Indicates a group divided by a group, an ester group or an ether group. R ² is a linear or branched alkyl group having 1 or more and 28 carbon atoms or less, and a linear or branched alkenyl group having 1 or more and 28 carbon atoms or less. , straight-chain or branched alkanol group having 1 to 28 carbon atoms, or the alkyl group, the alkenyl group or the alkanol group represents an amide group, an ester group or shed group with an ether group .R ³ and R ⁴ represents a linear or branched alkyl group having the same or different carbon atoms of 1 or more and 3 or less. A ⁻ indicates an anion which is a counter ion of an ammonium ion.)
In the general formula (1), ^{a preferred embodiment of R 1} is preferably a straight chain or branched chain alkyl group or an alkenyl group, and more preferably a straight chain alkyl group from the viewpoint of improving the sterilizing effect. Further, ^{the carbon number of R 1} is preferably 6 or more and 28 or less, and more preferably 12 or more and 18 or less, from the same viewpoint as described above. From the same viewpoint as described above, R ² is preferably a straight chain or branched chain alkyl group or an alkenyl group, and more preferably a straight chain alkyl group. Further, ^{the carbon number of R 2} is preferably 6 or more and 28 or less, and more preferably 12 or more and 18 or less, from the same viewpoint as described above. From the same viewpoint as described above, R ³ and R ⁴ are preferably a straight chain or branched chain alkyl group or an alkenyl group, and more preferably a straight chain alkyl group.
A ⁻ is not particularly limited as long as it is an anion. Examples thereof include inorganic or organic carboxylic acid ion, sulfonic acid ion, and halogen ion, and halogen ion is preferable, chloride ion and bromide ion are more preferable, and chloride ion is more preferable.

The content of the component (B) in the liquid preparation is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and 0 from the viewpoint of imparting high sterilizing property and stain removing property. It is preferably 0.4% by mass or less, and more preferably 0.15% by mass or less. The content of the component (B) is preferably 0.05% by mass or more and 0.4% by mass or less, and more preferably 0.1% by mass or more and 0.15% by mass or less in the liquid preparation. is there. When two or more kinds are contained as the component (B), the content of the component (B) is the total amount.

The mass ratio of the content of the component (A) to the content of the component (B) in the liquid preparation [(A) component / (B) component] is preferably 10/1 to 1/1. More preferably, it is ~ 3/1. If the content of the component (A) in the liquid preparation is too large, the feeling of use around the mouth and the finish of the clean surface deteriorate.

From the viewpoint of ensuring safety, the liquid preparation contains water as the component (C). Examples of water include distilled water, purified water and the like.

The content of the component (C) in the liquid preparation is preferably 97% by mass or more, more preferably 98% by mass or more, and preferably 99.7% by mass or less, preferably 99.5% by mass, from the viewpoint of imparting high safety. %% or less is more preferable. The content of the component (C) is preferably 97% by mass or more and 99.7% by mass or less, and more preferably 98% by mass or more and 99.5% by mass or less in the liquid preparation.

The liquid agent does not contain ethanol. As a result, the wet sheet is less irritating to the skin and can be safely used to wipe off dirt on the body such as hands, fingers, and around the mouth. "Ethanol-free" means that the liquid preparation does not contain ethanol at all and that the liquid preparation irreversibly mixes a small amount of ethanol. Specifically, the content of ethanol in the liquid preparation is 0.005% by mass. It means that it is less than.

From the viewpoint that the wet sheet is hard to dry and the wiping surface is smooth, and the take-out property is good when the wet sheet is taken out from the outlet of the encapsulation bag or the container containing the wet sheet, the liquid agent is a component (A), (B) And (C), it is preferable to contain silicone. Examples of the silicone include dimethylpolysiloxane, dimethylcyclopolysiloxane, methylphenylpolysiloxane, methylhydrogenpolysiloxane, higher alcohol-modified organopolysiloxane, and the like. These may be used alone or in combination of two or more.

The content of silicone in the liquid preparation is preferably 0.003% by mass or more, more preferably 0.005% by mass or more, and preferably 0.05% by mass or less, preferably 0.03, from the viewpoint of imparting the above effects. More preferably, the mass is %% or less. The range of the content of such silicone is preferably 0.003% by mass or more and 0.05% by mass or less, and more preferably 0.005% by mass or more and 0.03% by mass or less in the liquid preparation. When two or more types of silicone are contained, the content of silicone is the total amount.

In addition to the above-mentioned components, the liquid preparation may further contain components usually used in the art. Examples of such components include pH adjusters, polyhydric alcohols, preservatives, air fresheners, pigments and the like. These components can be used alone or in combination of two or more. The content of these in the liquid preparation is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, preferably 2.7% by mass or less, and more preferably 1.2% by mass or less. Examples of the pH adjuster include inorganic acids such as hydrochloric acid and sulfuric acid, inorganic bases such as sodium hydroxide and potassium hydroxide, and organic bases such as triisopropanolamine. As the pH adjuster, the acid is preferably an inorganic acid such as sulfuric acid, and the base is preferably an inorganic base such as sodium hydroxide or potassium hydroxide. Examples of the polyhydric alcohol include 1,3 butylene glycol, propylene glycol, dipropylene glycol, glycerin and the like. From the viewpoint of not inhibiting sterilization, it is preferable that the metal ion having a valence of 2 or more is not contained.

Moreover, the liquid agent may contain a surfactant other than the component (B). As the surfactant other than the component (B), a cationic surfactant, an anionic surfactant, and an amphoteric surfactant may be contained. Examples of the amphoteric surfactant include polyoxyethylene lauryl ether. The content of these surfactants in the liquid preparation is preferably not contained from the viewpoint of increasing the irritability to the skin because the permeability increases as the amount of the activator increases. However, from the viewpoint of being used for wiping stains and solubilizing fragrances, the content of these surfactants in the liquid preparation is preferably 0.01% by mass or more, preferably 0.05% by mass. The above is more preferable, 1.0% by mass or less is preferable, and 0.3% by mass or less is more preferable. In particular, from the viewpoint of suppressing discomfort such as dryness and dryness of the skin around the mouth, the content of the surfactant as a whole including the component (B) in the liquid preparation should be 1.0% by mass or less. preferable.

The liquid agent has a pH of 3.0 or more and less than 6.0 at 25 ° C. from the viewpoint of low irritation to the skin and safety. From the same viewpoint, the pH of the liquid agent at 25 ° C. is preferably 4.0 or more, more preferably 4.3 or more, preferably 5.5 or less, and more preferably 5.0 or less. As the pH measuring device, for example, LAQUA F-72 manufactured by HORIBA, Ltd. can be used.

From the viewpoint of safety, it is preferable that all the components contained in the liquid preparation of the wet sheet of the present invention satisfy the purity test described in the quasi-drug raw material standard 2006.

From the viewpoint of exhibiting the function of removing stains, the ratio of the liquid agent impregnated in the base material sheet is preferably 100% by mass or more, preferably 200% by mass or more, based on the mass of the base material sheet. Is more preferably 1000% by mass or less, and further preferably 500% by mass or less. The proportion of the impregnated liquid agent is preferably 100% by mass or more and 1000% by mass or less, and more preferably 200% by mass or more and 500% by mass or less with respect to the mass of the base sheet.

Examples of the base material sheet include non-woven fabrics, woven fabrics, and knitted fabrics. When a non-woven fabric is used as the base sheet, spunlace non-woven fabric, melt blown non-woven fabric, spunbond non-woven fabric, air-through non-woven fabric, resin bond non-woven fabric, needle punch non-woven fabric and the like can be used. Moreover, you may use the composite of these non-woven fabrics. Among these, spunlace non-woven fabric is preferably used from the viewpoint of being good to the touch. The basis weight of the non-woven fabric is preferably 20 g / m ² or more and 200 g / m ² or less, and more preferably 30 g / m ² or more and 100 g / m ² or less.

When the base sheet is a spunlace non-woven fabric, it is preferable that the spunlace non-woven fabric has a plurality of openings from the viewpoint of ease of wiping. The plurality of holes are preferably formed in a state of being arranged at uniform intervals in the plane direction of the sheet, but the intervals in the plane direction may be non-uniform. The opening is a hole penetrating the non-woven fabric. Area of the opening when viewed in plan is preferably not 0.3 mm ² or more 6 mm ² or less, more preferably 1.2 mm ² or more 1.9 mm ² or less. The shape of the opening is preferably circular or elliptical. In the case of an ellipse, when the MD direction (machine direction) is X and the CD direction (cross direction) is Y, the ratio of Y to X is preferably 0.1 or more and 1.0 or less, which is more preferable. Is 0.5 or more and 0.7 or less. The non-woven fabric is preferably a three-dimensional non-woven fabric in which the portion of the non-woven fabric in which the three-dimensional holes are formed is a concave portion and the other portion is a relatively convex portion. The number of holes per area in the base sheet is preferably 100 or more and 2000 or less, and more preferably 600 or more and 1000 or less per ^{100 cm 2.}

Examples of the fiber constituting the base material sheet include regenerated fiber, natural fiber, synthetic fiber and the like. From the viewpoint of ensuring safety, it is preferable that recycled fibers are contained. Examples of the recycled fiber include rayon fiber and acetate fiber, and rayon fiber is preferably used from the viewpoint of safety. Examples of natural fibers include cotton, hemp, collagen fibers and the like. Examples of the synthetic resin include polyamide, polyester, polyacrylic, and polyolefin.

When the base sheet contains rayon fibers, the liquid agent impregnated in the base sheet is difficult to dry, which is preferable. From this viewpoint, the proportion of rayon fiber in the base sheet is preferably 50% by mass or more, more preferably 70% by mass or more, preferably 100% by mass or less, and more preferably 90% by mass or less.

The wet sheet can be formed in a form in which a plurality of sheets are laminated and housed in an encapsulation bag made of a soft packaging material. Further, a long sheet can be formed in a rolled state and housed in a container.

The following additional notes will be further disclosed with respect to the above-described embodiments of the present invention.

<1>
A wet sheet in which a base sheet made of a fiber material is impregnated with a liquid agent.
The liquid agent is
(A) Lactic acid, citric acid, malic acid, acetic acid or salts of these acids, or combinations of two or more selected from these acids and salts,
(B) Quaternary ammonium salt-type surfactant, and (C) water,
A wet sheet containing, and ethanol-free, and having a pH of 3.0 or more and less than 6.0 at 25 ° C.

<2>
The wet sheet according to <1>, wherein the liquid agent contains 97% by mass or more and 99.7% by mass or less of water.
<3>
The wet sheet according to <1> or <2>, wherein the liquid agent further contains silicone.
<4>
The wet sheet according to any one of <1> to <3>, wherein all the components contained in the liquid preparation satisfy the quasi-drug raw material standard 2006.
<5>
The wet sheet according to any one of <1> to <4>, wherein the liquid agent is impregnated in a proportion of 100% by mass or more and 1000% by mass or less with respect to the mass of the base material sheet.
<6>
The wet sheet according to any one of <1> to <5>, wherein the base material sheet contains recycled fibers.
<7>
The wet sheet according to any one of <1> to <6>, wherein the base material sheet is made of a spunlace non-woven fabric in which rayon fibers and synthetic resin fibers are mixed.
<8>
The wet sheet according to <7>, wherein the base sheet has a ratio of rayon fibers of 50% by mass or more and 100% by mass or less, and a plurality of openings are formed.
<9>
The wet sheet according to any one of <1> to <8>, wherein the component (A) is fermented lactic acid.
<10>
The content of the component (A) in the liquid preparation is preferably 0.1% by mass or more, more preferably 0.2% by mass or more, and preferably 1.0% by mass or less, preferably 0.5% by mass or less. The wet sheet according to any one of <1> to <9>, which is more preferable.

<11>
The component (B) is a quaternary ammonium salt-type surfactant represented by the following general formula (1).
In the general formula (1), R ¹ is preferably a straight chain or branched chain alkyl group or an alkenyl group, more preferably a straight chain alkyl group, and ^{the carbon number of R 1} is preferably 6 or more and 28 or less, and 12 or more and 18 The following is more preferable, R ² is preferably a straight chain or branched chain alkyl group or an alkenyl group, more preferably a straight chain alkyl group, and ^{the carbon number of R 2} is preferably 6 or more and 28 or less, and 12 or more and 18 or less. More preferably, R ³ and R ⁴ are preferably a linear or branched alkyl group or an alkenyl group, more preferably a linear alkyl group, and A ⁻ is preferably a halogen ion, more preferably a chloride ion or a bromide ion. The wet sheet according to any one of <1> to <10>, wherein chlorinated ions are more preferable.
<12>
The content of the component (B) in the liquid preparation is preferably 0.05% by mass or more, more preferably 0.1% by mass or more, and preferably 0.4% by mass or less, preferably 0.15% by mass or less. The wet sheet according to any one of <1> to <11>, which is more preferable.
<13>
The mass ratio of the content of the component (A) to the content of the component (B) in the liquid preparation [component (A) / component (B)] is preferably 10/1 to 1/1. The wet sheet according to any one of <1> to <12>, more preferably 1 to 3/1.
<14>
The content of the component (C) in the liquid preparation is preferably 97% by mass or more, more preferably 98% by mass or more, preferably 99.7% by mass or less, and more preferably 99.5% by mass or less. The wet sheet according to any one of <1> to <13>.
<15>
The liquid preparation further contains silicone, and the content of the silicone in the liquid preparation is preferably 0.003% by mass or more, more preferably 0.005% by mass or more, and preferably 0.05% by mass or less, 0. The wet sheet according to any one of <1> to <14>, more preferably 0.03% by mass or less.
<16>
Examples of the silicone include dimethylpolysiloxane, dimethylcyclopolysiloxane, methylphenylpolysiloxane, methylhydrogenpolysiloxane, and higher alcohol-modified organopolysiloxane, which are used alone or in combination of two or more. The wet sheet according to 15>.
<17>
The liquid agent further contains a pH adjuster, a polyhydric alcohol, a preservative, a fragrance, and a pigment, and the content of these in the liquid agent is preferably 0.1% by mass or more, preferably 0.2% by mass or more. The wet sheet according to any one of <1> to <16>, more preferably 2.7% by mass or less, and more preferably 1.2% by mass or less.
<18>
The liquid preparation contains a surfactant other than the component (B), and the content of the surfactant in the liquid preparation is preferably 0.01% by mass or more, more preferably 0.05% by mass or more, and It is preferably 1.0% by mass or less, more preferably 0.3% by mass or less, and the content of the surfactant as a whole including the component (B) in the liquid preparation is preferably 1.0% by mass or less. The wet sheet according to any one of <1> to <17>.
<19>
The solution has a pH at 25 ° C. of 3.0 or more and less than 6.0, preferably 4.0 or more, more preferably 4.3 or more, and preferably 5.5 or less, and more preferably 5.0 or less. The wet sheet according to any one of <1> to <18>, which is preferable.

<20>
The ratio of the liquid agent impregnated in the base material sheet is preferably 100% by mass or more, more preferably 200% by mass or more, and 1000% by mass or less with respect to the mass of the base material sheet. The wet sheet according to any one of <1> to <19>, more preferably 500% by mass or less.
<21>
The base material sheet is a non-woven fabric, and the basis weight of the non-woven fabric is preferably 20 g / m ² or more and 200 g / m ² or less, and more preferably 30 g / m ² or more and 100 g / m ² or less. The wet sheet according to any one of> to <20>.
<22>
The wet sheet according to any one of <1> to <21>, wherein the base material sheet is a spunlace non-woven fabric, and a plurality of openings are formed in the spunlace non-woven fabric.
<23>
Area of the opening when viewed in plan is preferably not 0.3 mm ² or more 6 mm ² or less, more preferably 1.2 mm ² or more 1.9 mm ² or less, the wet sheet according to <22> ..
<24>
The shape of the opening is circular or elliptical, and when the aspect ratio of the ellipse is X in the MD direction (machine direction) and Y in the CD direction (cross direction), the ratio of Y to X is preferable. The wet sheet according to <22> or <23>, which is 0.1 or more and 1.0 or less, and more preferably 0.5 or more and 0.7 or less.
<25>
^{The number of holes per 100 cm 2} in the base material sheet is preferably 100 or more and 2000 or less, and more preferably 600 or more and 1000 or less, said <22> to <24>. The wet sheet described in any one of.
<26>
The base sheet contains rayon fibers, and the ratio of the rayon fibers to the base sheet is preferably 50% by mass or more, more preferably 70% by mass or more, and preferably 100% by mass or less, 90% by mass or less. The wet sheet according to any one of <1> to <25>, wherein it is more preferably mass% or less.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to such examples. Unless otherwise specified in the table, the content of each component indicates mass%.

[Examples 1 to 10 and Comparative Examples 1 to 5]
A liquid preparation containing the components shown in Table 1 below was used. A spunlace non-woven fabric was used as the base sheet. Wet sheets of Examples and Comparative Examples were produced by impregnating the spunlace non-woven fabric with a liquid agent at a ratio of 250% by mass with respect to the mass of the spunlace non-woven fabric. As the spunlace non-woven fabric, product number NW-RH3-40B manufactured by Daiwabo Polytec Co., Ltd. was used. The non-woven fabric had a size of 160 mm × 140 mm, a basis weight of 40 g / m ² , and a blending ratio of rayon fibers of 80% by mass. The blending ratio of the synthetic fiber of core: polypropylene / sheath: polyethylene was 20% by mass.

Details of the components of the liquid preparation shown in Table 1 are shown below. All of the components satisfied the purity test described in the quasi-drug raw material standard 2006.
・ Lactic acid: fermented lactic acid, product number PURAC ULTRAPURE90, manufactured by Purac (Tyland) ・ Citric acid: manufactured by Fuso Chemical Industry Co., Ltd. ・ Apple acid: manufactured by Fuso Chemical Industry Co., Ltd. ・ Acetic acid: manufactured by Wako Pure Chemical Industries, Ltd. Genuine Chemical Co., Ltd., quaternary ammonium salt type surface active agent: Sanizol C, Kao Co., Ltd., Surface active agent: Emargen 109P, Kao Co., Ltd., polyhydric alcohol: propylene glycol, ADEKA Co., Ltd., multivalent Alcohol: Dipropylene glycol, manufactured by ADEKA Co., Ltd. Silicone: Silicone KM-72, manufactured by Shinetsu Chemical Industry Co., Ltd. pH adjuster: Sodium hydroxide, manufactured by Nankai Chemical Co., Ltd. pH adjuster: sulfuric acid, Wako Pure Chemical Industries, Ltd. Company-made ethanol: Wako Junyaku Kogyo Co., Ltd.

For the wet sheets of Examples and Comparative Examples, the pH at 25 ° C. was measured using the above-mentioned measuring device. The wet sheet was evaluated by performing the sterilization test and antibacterial test shown below. In addition, the low irritation of the wet sheet to the skin and the feeling of use around the mouth were evaluated by the methods shown below. These results are shown in Table 1.

<Sterilization test>
Wet sheets of Examples and Comparative Examples were evaluated according to the "Bactericidal Performance Test of Wet Wipers" established by the Japan Clean Paper Cotton Industry Association.
Escherichia coli and Staphylococcus aureus were selected as the bacterial species. The viable cell count of each bacteria were respectively prepared in the 1.0 to 5.0 × 10 ⁹ cells / mL. 0.01 mL of each bacterial preparation was added dropwise onto the stainless steel plate. This plate was wiped off using the wet sheets of Examples and Comparative Examples. The wiping operation was performed by reciprocating 5 times on the plate at intervals of about 1 second with a load of 150 g applied to the wet sheet. After the wiping operation was completed, the wet sheet was left for 5 minutes, then transferred to a stomacher bag, and the bacteria were washed out under predetermined conditions. Further, the mixture was diluted under predetermined conditions, inoculated on SCDLP agar medium for general bacteria, and cultured at 35 ° C. for 1 day. After culturing, the viable cell count was measured, and the common logarithmic value of the cell count was defined as [B]. Further, the same operation was carried out using the control sheet, and the measured logarithmic value of the number of bacterial cells was defined as [A]. As a control sheet, a base sheet as a raw material for a wet sheet was used, and purified water was used as a liquid agent.
The sterilization activity value was quantified by the following formula.
Sterilization activity value = AB
A: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the control sheet B: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the wet sheets of Examples and Comparative Examples

Evaluation criteria:
a: Bactericidal activity value is 3.0 or more b: Bactericidal activity value is 2.0 or more and less than 3.0 d: Bactericidal activity value is less than 2.0 When the sterilization activity value is 2.0 or more, It means that the sterilization ability is high.

<Antibacterial test>
Wet sheets of Examples and Comparative Examples were evaluated in accordance with JIS Z2801 "Antibacterial Processed Product-Antibacterial Test Method / Antibacterial Effect".
Escherichia coli and Staphylococcus aureus were selected as the bacterial species. A sample plate having a size of 5 cm × 5 cm made of polyethylene resin was wiped twice with the wet sheets of Examples and Comparative Examples, and then dried. Each bacterial solution was dropped onto the surface of each dried sample plate and inoculated. Inoculation number was adjusted to 1.0 to 4.0 × 10 ⁵ per one sample plate. A polyethylene film was placed so as to adhere to the bacterial solution on the sample plate. The size of the film was 4 cm x 4 cm. Then, the cells were cultured for 24 hours in an environment of a temperature of 35 ° C. and a relative humidity of 90% or more. It was transferred to a stomacher bag and the bacteria were washed out under predetermined conditions. Further, it was diluted under predetermined conditions, inoculated on SCDLP agar medium for general bacteria, and cultured at 35 ° C. for 1 day. After culturing, the viable cell count was measured, and the common logarithmic value of the cell count was defined as [D]. Further, the same operation was carried out using the control sheet, and the measured logarithmic value of the number of bacterial cells was defined as [C]. As a control sheet, a base sheet as a raw material for a wet sheet was used, and purified water was used as a liquid agent.
The antibacterial activity value was quantified by the following formula.
Antibacterial activity value = CD
C: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the control sheet D: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the wet sheets of Examples and Comparative Examples

Evaluation criteria:
a: Antibacterial activity value is 3.0 or more b: Antibacterial activity value is 2.0 or more and less than 3.0 d: Antibacterial activity value is less than 2.0 When the antibacterial activity value is 2.0 or more, the antibacterial ability is high. Means that.

<Antibacterial test: Pollution condition test>
With reference to JIS Z2801 "Antibacterial processed products-Antibacterial test method / antibacterial effect", an antibacterial test method at the time of stain adhesion was prepared and evaluated. The difference from the conventional JIS Z2801 method is that 0.3% concentration of bovine serum albumin is added when preparing the bacterial solution. Since the addition of albumin can evaluate the antibacterial property at the time of stain adhesion, which could not be evaluated by JIS Z2801 "Antibacterial processed product-antibacterial test method / antibacterial effect", the wet sheets of Examples and Comparative Examples were evaluated. As a control sheet, a base sheet as a raw material for a wet sheet was used, and purified water was used as a liquid agent.

Antibacterial activity value = EF
E: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the control sheet F: Mean value of the common logarithmic value of the viable cell count in the results of three trials on the wet sheets of Examples and Comparative Examples

Evaluation criteria:
a: Antibacterial activity is 3.0 or more b: Antibacterial activity value is 2.0 or more d: Antibacterial activity value is less than 2.0

Antibacterial test: When the antibacterial activity value is 2.0 or more in the pollution condition test, the antibacterial effect is exhibited even in the presence of dirt and the antibacterial ability is a normal antibacterial test JIS Z2801 "Antibacterial processed product-Antibacterial test method / Antibacterial effect" The antibacterial property is higher than the result obtained in the above, and when the antibacterial activity value is 3.0 or more, it is positioned as the higher antibacterial property.

<Low irritation to the skin>
The back of the hand was wiped once per second with the wet sheets of Examples and Comparative Examples for a total of three round trips, and the low irritation to the skin was evaluated by three specialized panelists. The evaluation was made according to the following evaluation criteria in consultation with these panelists.

Evaluation criteria:
b: There is no discomfort such as a feeling of tension or dryness on the skin after wiping with a sheet d: There is a feeling of discomfort such as a feeling of tension or dryness on the skin after wiping with a sheet

<Usage around the mouth>
Using the wet sheets of the examples and comparative examples, wipe the area around the mouth once per second in a circular motion 5 times around the mouth to give the mouth a feeling of tightness and dryness after drying. Evaluated by name. The evaluation was made according to the following evaluation criteria in consultation with these panelists.

Evaluation criteria:
b: There is no discomfort such as a feeling of tension or dryness after wiping with a sheet d: There is a feeling of discomfort such as a feeling of tension or dryness after wiping with a sheet

As is clear from the results shown in Table 1, it was found that the wet sheets of the examples had high sterilizing ability and high antibacterial ability against Escherichia coli and Staphylococcus aureus, respectively. In addition, all of the wet sheets of the examples were found to be less irritating to the skin. From the above, it can be seen that the wet sheet of each example has both a high antibacterial and antibacterial effect and low irritation to the skin. On the other hand, the wet sheet of the comparative example had a weak sterilizing effect or was highly irritating to the skin.

The wet sheet of the example was evaluated by the methods shown below regarding the ease of taking out from the container and the finish of the wiped surface. These results are shown in Table 2.

<Removability from container>
The long wet sheet of the example was stored in a container in a rolled state, and the take-out property of the stored wet sheet was evaluated by three specialized panelists. The evaluation was made according to the following evaluation criteria in consultation with these panelists. The size of the long wet sheet was 160 mm × 140 mm, and it was wound from one end side in the longitudinal direction to be in a rolled state. As the container, a blow molded container manufactured by Toppan Printing Co., Ltd. for the lid and an injection molded cap with a hinge and a bottle manufactured by Hiroyuki Honda Co., Ltd. was used.

Evaluation criteria:
b: Easy to take out smoothly without abnormal noise or catching d: Hard to pull out due to abnormal noise or catching

<Finishing surface>
With the wet sheet of the example, the entire surface of the black plastic surface of 30 cm x 30 cm was wiped once per second for a total of three round trips, and the finish on the black plastic surface after drying was evaluated by three specialized panelists. did. The evaluation was made according to the following evaluation criteria in consultation with these panelists.

Evaluation criteria:
a: No visible change b: Slight wiping streaks (white streaks) are observed from one observation direction c: Wiping streaks (white streaks) are observed from several observation directions d: Clear whitening Is recognized

As is clear from the results shown in Table 2, among the wet sheets of Examples, the wet sheets of Examples 1 to 14 containing silicone were compared with the wet sheets of Example 15 containing no silicone. Therefore, it was found that the take-out property from the container was good, and the finish property of the cleaning surface was also good.

Claims (1)

For liquid preparations containing a quaternary ammonium salt type surfactant [component (B)] and water and not containing ethanol (excluding liquid preparations containing a quaternary ammonium salt and polyaminopropyl biguanide). The content of the component (B) in the solution is a combination of lactic acid, citric acid, ammonium, acetic acid or a salt of these acids, or a combination of two or more selected from these acids and salts [component (A)]. By adding the mass ratio of the content of the component (A) to the component (A) [component (A) / component (B)] at a ratio of 10 / 1-3 / 1, the pH at 25 ° C. is 3.0 or more and 6.0. A method for improving the sterilization property of the quaternary ammonium salt-type surfactant while reducing the amount to less than.