JP6898664B2 - Method for producing Sparassis crispa inoculum and method for producing Sparassis crispa - Google Patents

Description

The present invention relates to a method for producing an inoculum of Sparassis crispa capable of high-speed cultivation of Sparassis crispa and a method for producing Sparassis crispa using this inoculum.

Sparassis crispa, which is classified as a brown-rot fungus, is a rare mushroom that grows on coniferous trees such as larch, and is a promising edible mushroom that has a chewy and umami taste, a pure white color, and leaf button-like morphological characteristics. This Sparassis crispa succeeded in cultivating logs for the first time at Saitama Prefectural Kumagaya Agricultural High School on July 18, 1993 (Non-Patent Document 1). By adding the above, commercial cultivation by bottle cultivation became possible (Non-Patent Document 2 and Patent Document 1).

Since then, various measures have been taken regarding the cultivation method of Sparassis crispa, and it has been reported that the fruiting body formation rate is improved. (Patent Documents 2 to 7) However, in all the literature reports, in the cultivation of Sparassis crispa, the cultivation period from the inoculation of hyphae to the harvest was long, and high-speed cultivation could not be performed.

Yomiuri Shimbun, July 18, 1993 Prefecture North Area News Musroom Science and Biotechnology VOL. 1 No. 1 October, 1994 Research Report: Artificial cultivation of Sparassis crispa, pp. 15-17 Japanese Patent Application No. 9-228648 Japanese Unexamined Patent Publication No. 2005-21121 JP-A-2002-369621 Japanese Unexamined Patent Publication No. 2005-52068 Japanese Unexamined Patent Publication No. 2005-137263 JP-A-2007-104996 Japanese Unexamined Patent Publication No. 2008-230991

In view of the above-mentioned conventional drawbacks, an object of the present invention is to provide a method for producing a sparassis crispa inoculum that can significantly shorten the cultivation period and a method for producing a sparassis crispa using this inoculum.

The above and other objectives and novel features of the present invention will become more fully apparent when the following description is read in light of the accompanying drawings.
However, the drawings are for illustration purposes only and do not limit the technical scope of the present invention.

In order to achieve the above object, the method for producing a Hana Biratake inoculum according to claim 1 of the present invention includes a medium preparation step of preparing a well-ventilated solid medium and a Hana Biratake on the solid medium prepared in the medium preparation step. It is composed of a mycelium inoculation step of inoculating the mycelium of the above, and a culture step of culturing the inoculum in which the solid medium inoculated with the mycelium is subjected to aeration culture to form the offspring primordia or the juvenile body. the culture step, characterized Rukoto given by continuous or intermittent state 0.1 lux to about 5 lux light.

In the culture step of the method for producing a sparassis crispa inoculum according to claim 2, a 850 ml cultivation bottle is filled with 580 g of a solid medium, and about 0.1 liter to 0.5 liter of air is ventilated per day. It is characterized by culturing.

The method for producing Hana Biratake according to claim 3 includes a medium preparation step of preparing a solid medium having good air permeability, a mycelial inoculation step of inoculating the solid medium prepared in the medium preparation step with hyphae of Hana Biratake, and inoculation of hyphae. A culture step of culturing the inoculum that formed the offspring primordia or juveniles by aerial culture while giving weak light to the solid medium, and the inoculum cultivated in the culture step were continuously used in the medium or a new medium. Place inoculated state under high humidity environment, is composed of a generation step of growing fruiting, in the culture step, characterized Rukoto given by continuous or intermittent state 0.1 lux to about 5 lux light And.

In the culturing step of the method for producing Sparassis crispa according to claim 4, 580 g of a solid medium is filled in a 850 ml cultivation bottle, and about 0.1 liter to 0.5 liter of air is aerated per day for aeration culture. It is characterized by doing.

As is clear from the above description, the following effects can be obtained in the present invention.
(1) By giving weak light to the solid medium inoculated with hyphae and performing aeration culture, the formation rate of Sparassis crispa can be accelerated, and the number of days for cultivating Sparassis crispa and Sparassis crispa fruiting bodies can be significantly shortened.

1 to 5 are explanatory views showing a first embodiment of the present invention.
FIG. 3 is a process diagram of a Sparassis crispa inoculum and a method for producing Sparassis crispa showing the first embodiment. A table showing the experimental results of the preserved strain 1. A table showing the experimental results of the preserved strain 2. A table showing the experimental results of the preserved strain 3. A table showing the experimental results of the preserved strain 4.

Hereinafter, the present invention will be described in detail in accordance with the embodiments shown in the drawings for carrying out the present invention.
In the first embodiment for carrying out the present invention shown in FIGS. 1 to 5, 1 is a method for producing a sparassis crispa inoculum, and 2 is a method for producing a sparassis crispa inoculum. This is a method for producing Sparassis crispa, which produces Sparassis crispa using an inoculum.

The method 1 for producing the inoculum of Hana Biratake includes a medium preparation step 3 for preparing a well-ventilated solid medium, a mycelium inoculation step 4 for inoculating the solid medium prepared in the medium preparation step 3, and a mycelium inoculation step 4. It is composed of a culture step 5 in which a weak light is given to the solid medium and the inoculum is cultured by aeration culture.

In addition, the method 2 for producing Hana Biratake includes an inoculum inoculation step 6 in which the inoculum produced in the method 1 for producing Hana Biratake inoculum is inoculated into a medium, and a child body culture step in which the medium inoculated with the inoculum in the inoculation step 6 is cultured. After the child body culture step 7 is performed, the child body is placed in a high humidity environment to further perform the development step 8 for growing the child body.

In the medium preparation step 3, a solid medium containing a substance having good air permeability is prepared. The well-ventilated solid medium is a medium containing coarse-grained larch shavings, granular starches such as rice and wheat, and inorganic compounds such as vermiculite, akadama soil, and perlite, which have both water retention and breathability. Water is injected into these media, and the medium is prepared by, for example, stirring at about 65% using a stirring means, and then packing the medium into a device capable of inoculation and autoclave sterilization.

The solid medium thus prepared is inoculated with hyphae of about 4 to 6%, preferably about 5% of the amount of the medium, and the hyphae are evenly mixed with the medium equipment by stirring using a stirring means. I do. The medium inoculated with this hypha is lightly packed in an inoculum bottle to prepare a medium for inoculum, and the inoculum of Hanabiratake is cultivated in the next step (culture step 5).

In the culture step 5, aeration culture of the medium is performed. In this aeration culture, aseptic culture such as a filter is performed from the outside in a culture room in an environment of 20 to 23 ° C., which is the optimum temperature for hyphal growth of Hanabira bamboo, and a humidity of about 60 to 80%. Aseptic treatment is performed through the treatment means, and a small amount of clean air is blown into the medium for aeration.

In the present embodiment, the aeration amount when aseptic air is passed through the medium may be about 0.1 liter to 0.5 liter per day. Immediately after inoculation, the hyphae are allowed to grow on the medium equipment without aeration for about one week, and the amount of aeration is increased as the number of culture days elapses.

Also, preferably, the culture is carried out by giving a weak light. Here, the weak light means that light of about 0.1 to 5 lux is given, and may be continuous or intermittent administration.

When the aeration culture is continued, fruiting body primordium and fruiting body are formed, and the aeration culture is terminated at this stage. The fruiting body primordium and the juvenile body can be used as an inoculum of Sparassis crispa, and the culture of the inoculum of Sparassis crispa is terminated at this point.

If the aeration culture is continued as it is, the fruiting body primordium and the fruiting body in the medium will grow and cannot be used as an inoculum. Even after the above, it can be used as an inoculum for high-speed cultivation.

When cultivating Sparassis crispa using this inoculum, prepare a medium for cultivation. Following the above-mentioned steps, the inoculum inoculation step 6, the fruiting body culture step 7 and the development step 8 are performed.

In the inoculation step 6, a medium for cultivation is prepared, and the inoculum is inoculated into this medium.
As a material used for the culture medium for cultivation, for example, coniferous sawdust, hardwood sawdust and a mixture thereof, dried crushed grass such as corn cob, and the like can be used, and coniferous sawdust is preferable.

Especially, sawdust of larch is good, and fermented and aged one is the best. As a medium nutrient source, various starches can be used, and generally, dried and crushed starch is used. About 1% to 15% of these starches are added to the medium equipment, but when the concentration of starches is low, the fruiting bodies are white and beautiful, but the yield is low.

Further, the yield increases as the concentration of starch increases, but the fruiting body becomes thicker and becomes ocher, so about 7% to 10% is preferable. As for these starches, it is possible to cultivate Sparassis crispa only with the components of the bran produced in the process of refining cereals, but it is preferable that the amount of pure starch is large.

In this embodiment, nutrient sources such as wheat flour, corn flour, and barley flour are added, stirred, and then adjusted so that the water content is about 65%.

The next sterilization of the medium may be normal pressure sterilization or high pressure sterilization. In the case of atmospheric pressure sterilization, high pressure sterilization is preferable because the sterilization time becomes long and the efficiency is low. After the central portion of the medium exceeds 100 ° C., steam sterilization is performed at a temperature of about 118 ° C. to 120 ° C. for about 90 minutes, and after sterilization, the medium is immediately cooled to 20 ° C. or lower in a sterile environment.

Using the inoculum produced by the production method of the present invention, inoculate in a sterile space using an inoculator or the like in an amount such that the inoculation hole and the surface of the medium are thinly covered. The inoculation amount depends on the water content of the inoculum. The inoculation amount of sawdust inoculum is about 12 g to 25 g, but the inoculation amount of high-speed cultivation inoculum should be 20 g or more.

Next, the fruiting body culture step 7 will be described.
The culture room in which the fungus bed cultivation of mushrooms is generally carried out is dark culture, but in the fruiting body culture step 7 of the present embodiment, the culture is carried out in a weak light environment. The hyphal growth of Sparassis crispa and the growth rate of fruiting bodies are inversely proportional to each other in a strong light environment, but in a weak light environment, the hyphae grow normally and mushrooms grow at the same time. The weak light is a light environment having a brightness of about 0.1 lux to several lux.

It is important to give weak light evenly in order to evenly regulate the generation rate of fruiting bodies. The hyphal growth temperature of Sparassis crispa is about 15 ° C. to 28 ° C., but the hyphal elongation is good in the temperature range of 18 ° C. to 25 ° C., and the optimum culture temperature is about 20 ° C. to 23 ° C. The carbon dioxide concentration in the culture chamber is set not to exceed 1500 ppm for general mushrooms, but it does not cause any problem even if it exceeds 3000 ppm for Sparassis crispa.

After culturing for about 10 days, hyphae are thinly elongated on the shoulder opening of the bottle from the outside, and a white mycelial membrane can be confirmed on the upper part of the shoulder opening. As the days go by, the color of the hyphae on the shoulder opening increases in whiteness, and the mycelial membrane increases in width toward the bottle mouth. After culturing for about 2 weeks, hyphae grow about 15 mm from the shoulder opening. When the hyphae grow to this extent, remove the lid of the bottle and observe the growth of fruiting bodies. I'm worried about contamination, but even if I remove the lid and observe it at this time, it will not be contaminated.

If the fruiting body primordium and the fruiting body grow large and a lot of transparent spring water can be seen, the fruiting body culture step 7 is terminated and the process proceeds to the development step 8. There are some differences between inoculation of fruiting body primordium and inoculation of fruiting body. When the fruiting body primordium is inoculated, the fruiting body primordium grows round and large, and innumerable springs can be seen on it. When confirmed with a stereomicroscope, a juvenile entity is formed in the spring water part. In the case of infants, those that were flesh-colored become whiter and begin to grow larger.

The medium for which the fruiting body culture step 7 has been completed is transferred to a development chamber and subjected to a development step 8 for growing fruiting bodies in a high humidity environment.

The humidity environment of the cultivation room is preferably in the high humidity range of 80% to 100%, but since it varies greatly depending on the air volume, the humidity is maintained at 90% to 95% with a weak air volume. The temperature is optimally about 20 ° C to 23 ° C, which is the same as that of the culture room, although it grows at 15 ° C to 28 ° C. Note that yellowing occurs in fruiting bodies in an environment of 15 ° C or lower. The light environment may be weak light or bright light environment. There is no difference in growth between continuous lighting for 24 hours and intermittent lighting for 12 hours in light and dark. In the case of fluorescent lighting, Sparassis crispa, which is close to the light source, is dried by infrared rays, so it is better to use lighting that generates less heat, such as LEDs. In order to adjust the growth rate, it is necessary to be careful to give even brightness. In many cultivated mushrooms, the carbon dioxide concentration in the generation chamber is adjusted so that it does not exceed 1000 ppm, but unlike mushrooms with a handle, Sparassis crispa is insensitive to carbon dioxide concentration, and even if it exceeds 3000 ppm, it becomes the shape of fruiting bodies. Since it has the property of not causing any trouble, the ventilation frequency and time of the room may be reduced.

After cultivating for about a month, the petals will open wide, so try to harvest young fruiting bodies with little sporulation. When the cultivation period is long, the petals open wide and the volume of fruiting bodies increases, but the weight of fruiting bodies decreases compared to when they are young, and the color becomes ocher due to the formation of spores, so care must be taken at the time of harvest. Harvest 14 days after culturing and 30 days after cultivating. High-speed planting can be harvested in about 45 days after inoculation.

Cultivation experiments were carried out using the method for producing Sparassis crispa of the present invention. For comparison, cultivation was also carried out by the conventional method. Sparassis crispa used in this cultivation experiment was cultivated with a limited strain. In the case of wild strains and preserved strains, there are variations in sprout and fruiting body development, so strains with good sprout and fruiting body development were selected and bred.

As a result of genetic analysis of 82 strains of wild strains and preserved strains of Japanese Pinus densiflora species collected from various places, most of the strains are Sparassis latifolia, and some of them are in the Kansai and Chubu regions. Sparassis sp., The species name of which is distributed in the red pine belt is uncertain. Met. I also investigated about 200 strains on hand, but I could not find a species called Sparassis crispa. Sparassis crispa described in papers and patents on Sparassis crispa from Japan should be treated with caution.

This is an example using a preserved strain 1 of Sparassis crispa (Sparasis crispa latifolia produced in Japan).
Specimen No. No. 1 used a conventional cultivation method, and inoculated large sawdust of larch to produce sawdust inoculum. As the cultivation medium, 8% of crushed barley flour was added to sawdust of larch fermented for 3 years, and water was added to adjust the water content to 65%. 580 g of this was filled in a 850 ml polypropylene cultivation bottle, and one inoculation hole having a diameter of 20 mm was opened in the center of the cultivation bottle using a commercially available hole puncher, and then sterilized at 118 ° C. for 90 minutes. Then, after the medium temperature dropped to 20 ° C. or lower, 20 g of sawdust inoculum was inoculated into the inoculation hole and the surface of the medium. Culturing was carried out in a culture room having a temperature of 21 ° C. to 24 ° C. and a humidity of 60% to 70%. Fifty days after the inoculation, when the hyphae spread to almost the entire cultivation bottle, the fungus was scraped as an outbreak operation. About 10 days after the generation operation, fruiting bodies of Sparassis crispa were observed, and the formation rate was 100%. Remove the lid and move to the cultivation room, illuminate for 14 hours with humidity 95% to 97%, temperature 20 ° C to 23 ° C, brightness of 1 to 3 lux, and the child in an environment where the carbon dioxide concentration does not exceed 1500 ppm. The fruiting was grown and harvested 96 days after inoculation, which seems to have grown to the upper limit. The yield was 147 g per 32 test-cultivated plants, and the total yield was 4.7 kg.

Specimen No. 2 is also sample No. After culturing according to the same procedure as in No. 1, the fruiting body primordium grew large on the 26th day after inoculation, and a lot of spring water was observed, so that the fruiting body was moved to the cultivation room without any special development operation. The sprouting in the cultivation room varied because the development was not performed, but after 3 weeks, the growth was on average. Harvested 65 days after inoculation, which appears to have grown to the upper limit. The yield was 149 g per 32 test-cultivated plants, and the total yield was 4.8 kg.

Specimen No. In No. 3, the method for producing Sparassis crispa of the present invention was used, and 20 g of inoculum in which a large amount of fruiting body primordium was formed in advance was inoculated and cultured instead of inoculation with sawdust. Other than that, the sample No. The cells were cultured in the same procedure as in 1. On the 15th day after inoculation, the fruiting body primordium grew 100% larger, and a lot of spring water was seen, so it was moved to the outbreak room. Harvested 46 days after inoculation. The yield was 151 g per 32 test-cultivated plants, and the total yield was 4.8 kg.

Specimen No. In No. 4, the method for producing Sparassis crispa of the present invention was used, and 20 g of an inoculum in which a large amount of juveniles were formed in advance was inoculated and cultured. Other than that, the cells were cultured in the same procedure. On the 14th day after inoculation, the fruiting body primordium grew 100% larger and a lot of spring water was seen, so it was moved to the outbreak room and harvested 45 days after inoculation. The yield was 150 g per 32 test-cultivated plants, and the total yield was 4.8 kg.

The results of these cultivations are summarized in the table of FIG. Although there was no big difference in the yield of each sample, there was a big difference in the cultivation period, and the one using the method for producing Sparassis crispa of the present invention could be harvested in a shorter period of time than the conventional method. ..

Example 2 is an example using a preserved strain 2 of Sparassis crispa (Sparassis latifolia produced in Japan).

Specimen No. 1 is the sample No. 1 of Example 1. The cells were cultured according to the same procedure as in 1. 52 days after the inoculation, when the hyphae spread to almost the entire cultivation bottle, the fungus was scraped as an outbreak operation. The fruiting body formation of Sparassis crispa was observed 10 days after the development operation, and the formation rate was 100%. The cultivation process was also carried out in the same manner as in Example 1, and the crop was harvested on the 94th day after inoculation. The yield was 142 g per 32 test-cultivated plants, and the total yield was 4.5 kg.

Specimen No. 2 is the sample No. of Example 1. After culturing according to the same procedure as in No. 2, the fruiting body primordium grew large on the 27th day after inoculation, and a lot of spring water was observed, so that the fruiting body was moved to the cultivation room without any special development operation. The sprouting in the cultivation room varied because the development was not performed, but after 3 weeks, the growth was on average. Harvested 67 days after inoculation, which appears to have grown to the upper limit. The yield was 145 g per 32 test-cultivated plants, and the total yield was 4.6 kg.

Specimen No. 3 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 3, and on the 16th day after inoculation, the fruiting body primordia grew 100% larger, and a lot of spring water was observed, so they were moved to the development room. Harvested 47 days after inoculation. The yield was 143 g per 32 test-cultivated plants, and the total yield was 4.6 kg.

Specimen No. No. 4 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 4, and on the 14th day after inoculation, the fruiting body primordia grew 100% larger and a lot of spring water was observed, so they were moved to the development room. Harvested 45 days after inoculation. The yield was 146 g per 32 test-cultivated plants, and the total yield was 4.7 kg. Met.

The results of these cultivations are summarized in the table of FIG. Although there was no big difference in the yield of each sample, there was a big difference in the cultivation period, and the one using the method for producing Sparassis crispa of the present invention could be harvested in a shorter period of time than the conventional method. ..

Example 3 is an example using a preserved strain 3 of Sparassis crispa (Sparassis sp. Produced in Japan).

Specimen No. 1 is the sample No. 1 of Example 1. The cells were cultured according to the same procedure as in 1. 55 days after the inoculation, when the hyphae spread to almost the entire cultivation bottle, the fungus was scraped as an outbreak operation. The fruiting body formation of Sparassis crispa was observed 10 days after the development operation, and the formation rate was 100%. The cultivation process was also carried out in the same manner as in Example 1, and the crop was harvested on the 98th day. The fruiting bodies of the preserved strain 3 had wide petals and no cuts in the periphery of the petals, and clearly different characteristics from those of the preserved strain 1 and the preserved strain 2 were observed. The yield was 146 g per 32 test-cultivated plants, and the total yield was 4.7 kg.

Specimen No. 2 is the sample No. of Example 1. After culturing according to the same procedure as in No. 2, the fruiting body primordium grew large on the 27th day after inoculation, and a lot of spring water was observed, so that the fruiting body was moved to the cultivation room without any special development operation. The sprouting in the cultivation room varied because the development was not performed, but after 3 weeks, the growth was on average. Harvested 72 days after inoculation, which appears to have grown to the upper limit. The fruiting body of the preserved strain 3 had a characteristic that the petals were widely opened. The yield was 143 g per 32 test-cultivated plants, and the total yield was 4.6 kg.

Specimen No. 3 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 3, and on the 17th day after inoculation, the fruiting body primordia grew 100% larger, and a lot of spring water was observed, so they were moved to the development room. Harvested 48 days after inoculation. The fruiting body of the preserved strain 3 had a characteristic that the petals were widely opened. The yield was 141 g per 32 test-cultivated plants, and the total yield was 4.5 kg.

Specimen No. No. 4 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 4, and on the 15th day after inoculation, the larvae grew 100% larger and a lot of spring water was observed, so they were moved to the development room. Harvested 47 days after inoculation. The fruiting body of the preserved strain 3 had a characteristic that the petals were widely opened. The yield was 145 g per 32 test-cultivated plants, and the total yield was 4.6 kg.

The results of these cultivations are summarized in the table of FIG. There was no significant difference in the yield of each sample, but there was a significant difference in the cultivation period. The fruiting bodies of the preserved strain 3 had larger fruiting bodies than those of the preserved strains 1 and 2, and had a round characteristic with no cuts in the peripheral portion of the petals.

Example 4 is an example using a preserved strain 4 of Sparassis crispa (Sparassis crispa from Europe).

Specimen No. 1 is the sample No. 1 of Example 1. The cells were cultured according to the same procedure as in 1. 57 days after the inoculation, when the hyphae spread to almost the entire cultivation bottle, the fungus was scraped as an outbreak operation. 12 days after the development operation, fruiting bodies of Sparassis crispa were observed, and the formation rate was 100%. The cultivation process was also carried out in the same manner as in Example 1, and the crop was harvested on the 103rd day after inoculation. The fruiting bodies of the preserved strain 4 were found to have small petals. The yield was 158 g per 32 test-cultivated plants, and the total yield was 5.1 kg.

Specimen No. 2 is the sample No. of Example 1. The cells were cultured according to the same procedure as in 2. On the 29th day after inoculation, the fruiting body primordium grew large and a lot of spring water was seen, so it was moved to the cultivation room without any special generation operation. The sprouting in the cultivation room varied because the development was not performed, but after 3 weeks, the growth was on average. Harvested 78 days after inoculation, which appears to have grown to the upper limit. The fruiting bodies of the preserved strains showed the characteristic that the petals opened small. The yield was 160 g per 32 test-cultivated plants, and the total yield was 5.1 kg.

Specimen No. 3 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 3, and on the 17th day after inoculation, the fruiting body primordia grew 100% larger, and a lot of spring water was observed, so they were moved to the development room. Harvested 47 days after inoculation. The fruiting bodies of the preserved strain 4 had the characteristic that the petals were small and opened. The yield was 147 g per 32 test-cultivated plants, and the total yield was 4.7 kg.

Specimen No. No. 4 is the sample No. of Example 1. The cells were cultured according to the same procedure as in No. 4, and on the 15th day after inoculation, the larvae grew 100% larger and a lot of spring water was observed, so they were moved to the development room. Harvested 46 days after inoculation. The fruiting bodies of the preserved strain 4 showed a characteristic that the petals opened small. The yield was 148 g per 32 test-cultivated plants, and the total yield was 4.7 kg.

The results of these cultivations are summarized in the table of FIG. There was no significant difference in the yield of each sample, but there was a significant difference in the cultivation period. The fruiting bodies of the preserved strain 4 showed a characteristic that the fruiting bodies opened smaller than those of the preserved strains 1, 2, and 3. The cultivation period among the four strains was rather late, but the yield was high.

Comparing 16 different cultivation results using 4 strains and 4 different cultivation methods, it can be seen that 100% fruiting bodies are formed by using strains with good sprouting even if the strains and species are different. In addition, the conventional cultivation method in which the hyphae are spread over the entire medium and then the development operation is performed has a long cultivation period and is inefficient.

The cultivation period can be shortened by a cultivation method that promotes the growth of fruiting bodies while growing vegetative hyphae under the irradiation of dark light, which is a characteristic of Sparassis crispa. If the sparassis crispa cultivation method of the present invention in which the fruiting body is inoculated and the fruiting body is cultivated by using the seed bacterium on which the fruiting body or the fruiting body is formed, the cultivation can be performed at a higher speed, and in the cultivation on a commercial scale. It is possible to greatly reduce costs.

The present invention is used in the industry for producing Sparassis crispa.

1: Method of producing Sparassis crispa inoculum,
2: Sparassis crispa manufacturing method,
3: Medium preparation process, 4: Mycelial inoculation process,
5: Culture process, 6: Inoculation process,
7: Fruiting body culture step, 8: Development step.

Claims (4)

A medium preparation step of preparing a solid medium having good air permeability, a mycelium inoculation step of inoculating the solid medium prepared in the medium preparation step with the mycelium of Hanabiratake, and a mycelial culture while giving weak light to the solid medium inoculated with the mycelium. look including a culturing step of culturing inoculum carried out, in the culturing step, inoculum method of manufacturing Sparassis crispa give either continuously or intermittently state 0.1 lux to about 5 lux light. The first aspect of the culture step is to fill an 850 ml cultivation bottle with 580 g of the solid medium and aerate about 0.1 liter to 0.5 liter of air per day for aeration culture. The method for producing the inoculum of Sparassis crispa described. A medium preparation step for preparing a solid medium having good air permeability, a mycelium inoculation step for inoculating the solid medium prepared in the medium preparation step with the mycelium of Hanabiratake, and a mycelial culture while giving weak light to the solid medium inoculated with the mycelium. A culture step of culturing the inoculum, an inoculum inoculation step of inoculating the inoculum cultivated in the culture step into a cultivation medium, and a child entity culturing the cultivation medium inoculated with the inoculum in the inoculum inoculation step. and culturing step, seen including a generating step of growing the fruit body under high humidity environment after the child entity culture step, in the culturing step, either continuous or intermittent state 0.1 lux to about 5 lux light How to make Hana Biratake given in. The third aspect of the culture step is characterized in that a 850 ml cultivation bottle is filled with 580 g of the solid medium, and aeration culture is performed by aerating about 0.1 liter to 0.5 liter of air per day. The method for manufacturing Sparassis crispa described.

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