JP6933802B2 - Cell storage container - Google Patents

Description

An embodiment of the present invention relates to a cell containing container for containing cells.

In regenerative medicine, tissues and organs are artificially created by cell culture. Patent Documents 1 and 2 disclose techniques for recovering cells cultured in a culture vessel. Patent Document 1 discloses a technique for recovering cells exfoliated from an adhesive surface. Patent Document 2 discloses a technique for preventing leakage of a liquid substance such as a culture solution when a liquid substance such as a culture solution is supplied or discharged or cells are cultured in a state where a three-dimensional container is laid down. .. Patent Document 3 discloses a technique relating to a jig for cell culture and an automatic medium exchange system that automates a medium exchange operation.

Japanese Unexamined Patent Publication No. 2003-235541 JP-A-2009-34078 Japanese Unexamined Patent Publication No. 2015-84686

In these cell culture systems, a closed cell culture container is used to prevent contamination by bacteria, viruses, etc. from the outside. For example, in adhesive cell culture, when harvesting cells adhered to the bottom surface of a culture vessel, the adhesive force between the cells and the bottom surface of the culture vessel is first reduced by using an enzyme or the like, and then the cells are used as a medium. A method of flushing with the culture medium and cell suspension is used. The medium, culture medium, and cell suspension flushed from the closed-type cell culture vessel can be contained in a closed-type cell storage container that contains the medium, culture medium, and cell suspension. Supply and discharge of medium, culture medium, and cell suspension to and from the containment vessel is realized by adjusting the air pressure inside the containment vessel. The air pressure in the containment vessel is adjusted by supplying and exhausting gas in the containment vessel. Gas supply and exhaust in the containment vessel are performed through the vents provided in the containment vessel. The inside of the storage container is communicated to the outside of the storage container (specifically, a pump for supplying and exhausting gas, etc.) through a vent. In this case, when the medium, the culture medium, and the cell suspension are contained in the storage container, the contained medium, the culture medium, and the cell suspension leak to the outside of the storage container through the vent. May be done. Therefore, a technique for avoiding a situation in which the medium, the culture solution, and the cell suspension contained in the storage container leak to the outside of the storage container is desired.

In one aspect, a cell containment vessel is provided. This cell storage container includes a bottom wall portion, a ceiling wall portion, a side wall portion, a partition wall portion, a supply / discharge portion, and a ventilation portion, and the bottom wall portion, the ceiling wall portion, and the side wall portion define the internal space of the cell storage container. However, the bottom wall portion and the ceiling wall portion are provided apart from each other via the side wall portion, the side wall portion extends from the bottom wall portion to the ceiling wall portion, and the supply / discharge portion is provided on the side wall portion. It is provided so that the internal space and the space outside the cell storage container are communicated with each other and arranged on the side of the bottom wall portion. Connected, the vent is provided on the side wall to communicate the internal space with the space outside the cell containment vessel, and the bulkhead extends from the bottom wall to the ceiling wall in the internal space for ventilation. An isolated area is defined in front of the portion so as to cover the ventilation portion, and the two ends of the partition wall portion extending from the bottom wall portion toward the ceiling wall portion are joined to the side wall portion, and the two ends of the partition wall portion are joined. An opening of a ventilation portion on the side of the isolation region is arranged between the spaces, a first gap region is provided between the partition wall portion and the ceiling wall portion, and the first gap region is provided in the internal space. Communicate between the inside of the isolation area and the outside of the isolation area.

According to the above aspect, the ventilation portion and the internal space of the cell containment vessel (more specifically, the interior space) are provided through the isolation region defined by the partition wall portion and the gap region provided between the partition wall portion and the ceiling wall portion. Since the area (the area excluding the isolated area from the internal space) is communicated with the internal space, the air pressure in the internal space (more specifically, the area excluding the isolated area from the internal space) can be adjusted through the ventilation portion. By adjusting the pressure in the interior space (more specifically, the region excluding the isolation region from the interior space), the medium, culture medium, and cells for the interior space (more specifically, the region excluding the isolation region from the interior space). The supply and discharge of the suspension is well performed through the supply and discharge section. Even when the medium, culture medium, and cell suspension are contained in the internal space (more specifically, the region excluding the isolated region from the internal space), the partition wall covers the opening of the ventilation portion, so that the medium is used. , The inflow of the culture medium and the cell suspension into the aeration part is sufficiently suppressed by the partition part.

In one embodiment, the cell containment vessel further comprises a protrusion, which extends from the ceiling wall to the bottom wall in the interior space and along the bulkhead outside the isolation area in the interior space. It extends and is provided so as to cover the first gap region, the protrusion and the partition wall form a labyrinth structure, and a second gap region is provided between the protrusion and the partition wall. There is. In this way, the protrusion covers the first gap region provided between the ceiling wall portion and the partition wall portion, and further, the second gap region provided between the protrusion portion and the partition wall portion. Since the labyrinth structure is formed on the side of the ceiling wall, the medium, culture medium, and cell suspension are contained in the internal space (more specifically, the region excluding the isolated region from the internal space). However, the inflow of the medium, the culture medium, and the cell suspension into the aeration part can be suppressed more sufficiently.

In one embodiment, the surface of the bottom wall portion is inclined so as to approach the ceiling wall portion from the side of the supply / discharge portion toward the side of the side wall portion. The surface of the bottom wall portion according to one embodiment is divided into a plurality of surface regions, each of the plurality of surface regions is flat, and approaches the ceiling wall portion from the side of the supply / discharge portion toward the side of the side wall portion. It is inclined like. Alternatively, the surface of the bottom wall portion according to the embodiment is a curved surface. In this way, since the surface of the bottom wall portion is inclined from the side of the supply / discharge portion toward the side of the side wall portion, the cell containing container is placed on the horizontal plane and the bottom wall portion and the ceiling wall portion extend horizontally. When the medium, culture medium, and cell suspension contained in the internal space (more specifically, the region excluding the isolated region from the internal space) are discharged from the supply / discharge section in the state of being (horizontally placed). , Medium, culture medium, cell suspension can be well discharged from the supply and discharge section.

As described above, there is provided a technique for avoiding a situation in which the medium, the culture solution, and the cell suspension contained in the storage container leak to the outside of the storage container.

FIG. 1 is a front perspective view on the plane side according to the first embodiment. FIG. 2 is a rear perspective view on the plane side according to the first embodiment. FIG. 3 is a right side view according to the first embodiment. FIG. 4 is a left side view according to the first embodiment. FIG. 5 is a plan view according to the first embodiment. FIG. 6 is a bottom view according to the first embodiment. FIG. 7 is a front view according to the first embodiment. FIG. 8 is a rear view according to the first embodiment. FIG. 9 is a rear perspective view of the bottom surface according to the first embodiment. FIG. 10 is a front perspective view on the bottom surface side according to the first embodiment. FIG. 11 is an enlarged CC sectional view of the DD and EE portions according to the first embodiment. FIG. 12 is an enlarged BB cross-sectional view of the DD portion according to the first embodiment. FIG. 13 is a cross-sectional view taken along the line AA according to the first embodiment. FIG. 14 is a diagram showing the shape of the surface of the bottom wall portion on the side of the internal space according to the first embodiment. FIG. 15 is a diagram showing the configuration and arrangement of the partition wall portion according to the first embodiment. FIG. 16 is a front perspective view on the plane side according to the second embodiment. FIG. 17 is a rear perspective view on the plane side according to the second embodiment. FIG. 18 is a right side view according to the second embodiment. FIG. 19 is a left side view according to the second embodiment. FIG. 20 is a plan view according to the second embodiment. FIG. 21 is a bottom view according to the second embodiment. FIG. 22 is a front view according to the second embodiment. FIG. 23 is a rear view according to the second embodiment. FIG. 24 is a rear perspective view of the bottom surface according to the second embodiment. FIG. 25 is a front perspective view on the bottom surface side according to the second embodiment. FIG. 26 is an enlarged CC sectional view of the DD and EE portions according to the second embodiment. FIG. 27 is an enlarged BB cross-sectional view of the DD portion according to the second embodiment. FIG. 28 is a cross-sectional view taken along the line AA according to the second embodiment. FIG. 29 is a diagram showing the shape of the surface of the bottom wall portion on the side of the internal space according to the second embodiment. FIG. 30 is a rear perspective view on the reference bottom side according to the first embodiment. FIG. 31 is a reference enlarged CC sectional view in the DD and EE portions according to the first embodiment. FIG. 32 is a reference enlarged BB cross-sectional view in the DD portion according to the first embodiment. FIG. 33 is a cross-sectional view taken along the line AA according to the first embodiment. FIG. 34 is a rear perspective view of the reference bottom surface side according to the second embodiment. FIG. 35 is a reference enlarged CC sectional view in the DD and EE portions according to the second embodiment. FIG. 36 is a reference enlarged BB cross-sectional view in the DD portion according to the second embodiment. FIG. 37 is a cross-sectional view taken along the line AA according to the second embodiment.

Hereinafter, various embodiments will be described in detail with reference to the drawings. In addition, the same reference numerals are given to the same or corresponding parts in each drawing.

(First Embodiment)
The configuration of the cell storage container 1 according to the first embodiment is shown in FIGS. 1 to 15. FIG. 1 is a front perspective view on the plane side according to the first embodiment. FIG. 2 is a rear perspective view on the plane side according to the first embodiment. FIG. 3 is a right side view according to the first embodiment. FIG. 4 is a left side view according to the first embodiment. FIG. 5 is a plan view according to the first embodiment. FIG. 6 is a bottom view according to the first embodiment. FIG. 7 is a front view according to the first embodiment. FIG. 8 is a rear view according to the first embodiment. FIG. 9 is a rear perspective view of the bottom surface according to the first embodiment. FIG. 10 is a front perspective view on the bottom surface side according to the first embodiment. FIG. 11 is an enlarged CC sectional view of the DD and EE portions according to the first embodiment. FIG. 12 is an enlarged BB cross-sectional view of the DD portion according to the first embodiment. FIG. 13 is a cross-sectional view taken along the line AA according to the first embodiment. FIG. 14 is a diagram showing the shape of the surface of the bottom wall portion on the side of the internal space according to the first embodiment. FIG. 15 is a diagram showing the configuration and arrangement of the partition wall portion according to the first embodiment.

The cell storage container 1 contains, for example, pluripotent stem cells such as iPS cells and ES cells, cartilage cells such as bone marrow stromal cells (MSC), and various adhesive (adherent) cells such as dendritic cells. It can be used to contain media, culture solutions, and cell suspensions used for culturing. In addition, the cell storage container 1 can be used for storing a medium, a culture solution, a cell suspension used for culturing floating cells such as blood cell lines, preferably T cells and B cells, or as a culture container for cell culture. Can be used. The cell storage container 1 includes a storage portion 1A and a frame portion 1B. In one embodiment, the material of the cell containment vessel 1 may include a (transparent) resin having high light transmission, such as polystyrene. When the cell storage container 1 has light transmission, it becomes easy to optically observe the cells being cultured in the storage portion 1A of the cell storage container 1 from the outside of the cell storage container 1. The cell storage container 1 can be integrally formed by, for example, injection molding.

The accommodating portion 1A includes a bottom wall portion 1A1, a ceiling wall portion 1A2, a side wall portion 1A3, a partition wall portion 1A4, a supply / discharge portion 1A5, and a ventilation portion 1A6. The accommodating portion 1A further includes a protrusion 1A7. In the storage portion 1A, the bottom wall portion 1A1, the ceiling wall portion 1A2, and the side wall portion 1A3 define the internal space SP of the storage portion 1A of the cell storage container 1. The internal space SP includes the main space SP1 and the isolated area ER. In the internal space SP, the main space SP1 and the isolation area ER are separated by the partition wall portion 1A4. In the internal space SP, the main space SP1 and the isolation region ER communicate with each other via the gap region VD1 (first gap region) and the gap region VD2 (second gap region), which will be described later.

The bottom wall portion 1A1 and the ceiling wall portion 1A2 are provided so as to be separated from each other via the side wall portion 1A3. The bottom wall portion 1A1 and the ceiling wall portion 1A2 extend so as to face each other. The side wall portion 1A3 extends from the bottom wall portion 1A1 to the ceiling wall portion 1A2. The side wall portion 1A3 is provided along the edge of the bottom wall portion 1A1 and is provided along the edge of the ceiling wall portion 1A2. The side wall portion 1A3 extends from the edge of the bottom wall portion 1A1 to the edge of the ceiling wall portion 1A2. The shape of the box forming the storage portion 1A of the cell storage container 1 is defined by the bottom wall portion 1A1, the ceiling wall portion 1A2, and the side wall portion 1A3.

The supply / discharge portion 1A5 is provided on the side wall portion 1A3. The supply / discharge portion 1A5 is arranged closer to the bottom wall portion 1A1 (on the side of the bottom wall portion). The supply / discharge unit 1A5 communicates the main space SP1 of the internal space SP with the space outside the storage unit 1A of the cell storage container 1. The surface 1A1f of the bottom wall portion 1A1 faces the internal space SP and is connected to the inner surface of the supply / discharge portion 1A5. In one embodiment, the surface 1A1f can be smoothly connected to the inner surface of the supply / discharge portion 1A5 without a step. The supply / discharge unit 1A5 includes an opening 1A5a and an opening 1A5b. The inner surface of the supply / discharge portion 1A5 extends from the opening 1A5a to the opening 1A5b. The opening 1A5a is on the side of the internal space SP and faces the main space SP1 of the internal space SP. The opening 1A5b is on the side of the space outside the storage portion 1A of the cell storage container 1 and faces this space. The supply / discharge unit 1A5 is a flow passage for a liquid (hereinafter, may be simply referred to as a liquid) such as a medium, a culture solution, and a cell suspension. A sealing member made of, for example, an elastic body (not shown) is inserted into the opening of the supply / discharge portion 1A5, and the liquid can be airtightly supplied and discharged to the main space SP1 of the accommodating portion 1A via the sealing member. In a state where the cell storage container 1 is placed on a horizontal plane (in this case, the bottom wall portion 1A1 extends along the horizontal plane, and the surface 1A1f of the bottom wall portion 1A1 is from the side wall portion 1A3 to the supply / discharge portion 1A5. The liquid supplied from the outside of the cell storage container 1 via the supply / discharge portion 1A5 is inclined toward the side of the opening 1A5a (inclined so as to be separated from the ceiling wall portion 1A2). Further, when the opening 1A5b) is closed, it can be accommodated in the main space SP1.

The ventilation portion 1A6 is provided on the side wall portion 1A3. In one embodiment, the ventilation portion 1A6 may be arranged closer to the bottom wall portion 1A1. The ventilation portion 1A6 communicates the isolation region ER of the internal space SP with the space outside the storage portion 1A of the cell storage container 1. The ventilation portion 1A6 includes an opening 1A6a and an opening 1A6b. The inner surface of the vent 1A6 extends from the opening 1A6a to the opening 1A6b. The opening 1A6a is on the side of the internal space SP and faces the isolated area ER of the internal space SP. The opening 1A6b is on the side of the space outside the storage portion 1A of the cell storage container 1 and faces this space. The ventilation portion 1A6 is a flow passage for a gas (a gas used for adjusting the internal pressure of the main space SP1 and may be simply referred to as a gas below). Gas can be supplied and discharged to the main space SP1 of the accommodating portion 1A through the venting portion 1A6.

In the internal space SP of the accommodating portion 1A, a sealing member made of, for example, an elastic body is inserted into which the opening 1A5a (further opening 1A5b) of the air supply / discharge portion 1A5 and the opening 1A6a (further opening 1A6b) of the ventilation portion 1A6 are not shown. Airtightness is maintained in the closed state. Access to the internal space SP of the storage portion 1A from the outside of the cell storage container 1 can be performed while maintaining the airtight state by inserting the needle-shaped connecting member into the sealing member described above.

The partition wall portion 1A4 extends from the bottom wall portion 1A1 toward the ceiling wall portion 1A2 in the internal space SP (see, for example, FIG. 15 in particular). The partition wall portion 1A4 is joined to the bottom wall portion 1A1. In the internal space SP, the partition wall portion 1A4 covers the ventilation portion 1A6 in front of the ventilation portion 1A6 (more specifically, covers the opening 1A6a of the ventilation portion 1A6) from the main space SP1 and the isolation region ER. And demarcate. The partition wall portion 1A4 is joined to the bottom wall portion 1A1 and the side wall portion 1A3 so as to define the main space SP1 and the isolation region ER. The partition wall portion 1A4 includes an end side portion 1A4a, an end side portion 1A4b, an end side portion 1A4c, and an end side portion 1A4d. As described above, the partition wall portion 1A4 has a plate-like shape having four sides in a plan view. The partition wall portion 1A4 has a surface 1A4f. The surface 1A4f is defined by an end edge portion 1A4a, an end edge portion 1A4b, an end edge portion 1A4c, and an end edge portion 1A4d. The surface 1A4f faces the main space SP1 in the internal space SP. The back surface of the partition wall portion 1A4 with respect to the surface surface 1A4f faces the isolation region ER in the internal space SP.

The extending direction of the end side portion 1A4a and the end side portion 1A4b and the extending direction of the end side portion 1A4c and the end side portion 1A4d intersect with each other. The end side portion 1A4a and the end side portion 1A4b extend side by side from the end side portion 1A4c to the end side portion 1A4d. The end edge portion 1A4a is joined to the bottom wall portion 1A1 and is inclined with respect to the horizontal plane along the surface 1A1f of the bottom wall portion 1A1 (the surface 1A1f is inclined with respect to the horizontal plane when the cell storage container 1 is placed on the horizontal plane). Extends (along). The end side portion 1A4b faces the ceiling wall portion 1A2 and extends along the ceiling wall portion 1A2 at a position close to the ceiling wall portion 1A2. The end side portion 1A4c and the end side portion 1A4d extend side by side from the end side portion 1A4a to the end side portion 1A4b. Both one end of the end side portion 1A4c and one end of the end side portion 1A4d are joined to the bottom wall portion 1A1. The end side portion 1A4c and the end side portion 1A4d extend from the bottom wall portion 1A1 toward the ceiling wall portion 1A2, and are joined to the side wall portion 1A3, respectively.

In the side wall portion 1A3, the opening 1A6a of the ventilation portion 1A6 on the side of the internal space SP is arranged between the end side portion 1A4c and the end side portion 1A4d. A gap region VD1 is provided between the partition wall portion 1A4 and the ceiling wall portion 1A2. The gap region VD1 is arranged between the end side portion 1A4b of the partition wall portion 1A4 and the ceiling wall portion 1A2. The gap region VD1 communicates with the inside of the isolation region ER and the outside of the isolation region ER (main space SP1) in the internal space SP.

The protrusion 1A7 extends from the ceiling wall portion 1A2 toward the bottom wall portion 1A1 in the internal space SP (specifically, the main space SP1). The protrusion 1A7 extends along the partition wall 1A4 (more specifically, the surface 1A4f of the partition wall 1A4) on the outside of the isolation region ER in the internal space SP (on the side of the main space SP1). The protrusion 1A7 is provided so as to cover the gap region VD1 in the internal space SP (specifically, the main space SP1). The protrusion 1A7 and the partition wall 1A4 form a labyrinth structure. A gap region VD2 is provided between the protrusion 1A7 and the partition wall 1A4.

The gas supplied from the outside of the cell storage container 1 to the internal space SP (specifically, the main space SP1) of the storage unit 1A via the ventilation unit 1A6 is supplied from the opening 1A6b of the ventilation unit 1A6 to the opening 1A6a and the isolation region ER. The main space SP1 is reached through the gap region VD1 and the gap region VD2 in this order. On the other hand, in the labyrinth structure provided between the protrusion 1A7 and the partition wall 1A4, the liquid contained in the main space SP1 invades the isolation region ER via the gap region VD2 and reaches the ventilation portion 1A6. Avoid the situation. Due to this labyrinth structure, when a liquid such as a medium is supplied to the main space SP1 of the storage unit 1A, or when the cell storage container 1 in a state where the main space SP1 is filled with a liquid such as a medium is transported, the isolation region ER The ingress of liquid into the medium is effectively prevented.

In the state where the cell storage container 1 is placed on the horizontal plane, the supply and discharge of the liquid to the main space SP1 via the supply / discharge unit 1A5 is performed through the ventilation unit 1A6 without the liquid invading the ventilation unit 1A6. It can be done by increasing or decreasing (adjusting) the air pressure in the internal space SP by supplying and discharging the gas.

The configuration of the bottom wall portion 1A1 will be described in more detail. The bottom wall portion 1A1 may have a substantially uniform thickness in one embodiment. Therefore, it is shown that the back surface of the bottom wall portion 1A1 with respect to the surface 1A1f is substantially parallel to the surface 1A1f (in the present specification, substantially parallel is not necessarily limited to parallel in a geometrically strict sense. Similarly.) The back surface is inclined in the same manner as the front surface 1A1f. The surface 1A1f of the bottom wall portion 1A1 is inclined so as to approach the ceiling wall portion 1A2 from the side of the opening 1A5a of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3. In a state where the cell storage container 1 is placed on a horizontal plane, the surface 1A1f is lowered (separated from the ceiling wall portion 1A2) from the side of the side wall portion 1A3 toward the side of the opening 1A5a of the supply / discharge portion 1A5. To), it is inclined. In the state where the cell storage container 1 is placed on the horizontal plane, the ceiling wall portion 1A2 extends horizontally, and the surface 1A1f of the bottom wall portion 1A1 extends with respect to the horizontal plane (relative to the extending direction of the ceiling wall portion 1A2). It is tilted. The shape of the surface 1A1f is, for example, that if a sphere is placed on the surface 1A1f at any position on the surface 1A1f while the cell storage container 1 is placed on a horizontal plane, the sphere rolls on the surface 1A1f by gravity. It is provided so as to reach the opening 1A5a of the exhaust portion 1A5. Therefore, the cell storage container 1 is placed on a horizontal plane, the opening 1A5a of the supply / discharge portion 1A5 is closed, and the opening 1A5a is opened from the state where the liquid is stored in the main space SP1 and is stored in the main space SP1. When the liquid is discharged to the outside via the supply / discharge portion 1A5, the surface 1A1f of the bottom wall portion 1A1 is inclined as described above, so that the liquid is caused by gravity without tilting the cell storage container 1. It can flow on the surface 1A1f, be efficiently collected in the supply / discharge section 1A5, and be discharged to the outside from the supply / discharge section 1A5.

Further, the surface 1A1f of the bottom wall portion 1A1 is divided into a plurality of surface regions, each of the plurality of surface regions is flat, and the ceiling wall portion increases from the side of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3. It can be tilted closer to 1A2. In the first embodiment, specifically, as shown in FIGS. 6, 9, 10, 13, and 14, the surface 1A1f of the bottom wall portion 1A1 has two surface regions (surface region PE1a, surface). It can be divided into regions PE1b). The surface 1A1f includes a surface region PE1a and a surface region PE1b. Each of the surface region PE1a and the surface region PE1b is flat and can be inclined so as to approach the ceiling wall portion 1A2 from the side of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3.

The frame portion 1B includes a bottom portion 1B1 and a wall portion 1B2. The accommodating portion 1A is provided on the bottom portion 1B1. The bottom portion 1B1 has a plate-like shape. The accommodating portion 1A is arranged substantially in the center of the bottom portion 1B1. The wall portion 1B2 is provided along the outer edge of the bottom portion 1B1. The wall portion 1B2 extends along the side wall portion 1A3 of the accommodating portion 1A substantially parallel to the side wall portion 1A3. The opening 1A5b of the supply / discharge portion 1A5 and the opening 1A6b of the ventilation portion 1A6 are provided in the wall portion 1B2.

According to the cell storage container 1 according to the above-described embodiment, ventilation is performed through the isolation region ER defined by the partition wall portion 1A4 and the gap region VD1 provided between the partition wall portion 1A4 and the ceiling wall portion 1A2. Since the portion 1A6 and the main space SP1 of the internal space SP are communicated with each other, the air pressure of the main space SP1 of the internal space SP can be adjusted via the ventilation portion 1A6. By adjusting the air pressure in the main space SP1, the supply and discharge of the liquid to the main space SP1 is satisfactorily performed via the supply / discharge unit 1A5. Even when the liquid is stored in the main space SP1, the partition wall portion 1A4 covers the opening 1A6a of the ventilation portion 1A6, so that the liquid stored in the main space SP1 flows into the ventilation portion 1A6 by the partition wall portion 1A4. Sufficiently suppressed.

Further, the gap region VD1 provided between the ceiling wall portion 1A2 and the partition wall portion 1A4 is covered by the protrusion 1A7, and further, the gap region VD2 provided between the protrusion 1A7 and the partition wall portion 1A4 covers the gap region VD1. Since the labyrinth structure is formed on the side of the ceiling wall portion 1A2, even when the liquid is contained in the main space SP1 of the internal space SP, the inflow of the liquid into the ventilation portion 1A6 can be sufficiently suppressed. In this case, if the surface (liquid level) of the liquid contained in the main space SP1 is located at a height lower than the surface of the protrusion 1A7, the liquid overflows from the partition wall portion 1A4 and invades the ventilation portion 1A6. There is no. Further, when the cell storage container 1 in which the main space SP1 is filled with the liquid is transported, the invasion of the liquid into the isolation region ER is effectively prevented.

Further, since the surface 1A1f of the bottom wall portion 1A1 is inclined so as to approach the ceiling wall portion 1A2 from the side of the opening 1A5a of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3, the cell storage container 1 is placed on the horizontal plane. When the liquid contained in the main space SP1 of the internal space SP is discharged from the supply / discharge part 1A5 in a state where the bottom wall part 1A1 and the ceiling wall part 1A2 are placed and extend horizontally, the liquid is discharged from the supply / discharge part 1A5. Can be well discharged from.

(Second Embodiment)
The configuration of the cell storage container 1 according to the second embodiment is shown in FIGS. 16 to 29. FIG. 16 is a front perspective view on the plane side according to the second embodiment. FIG. 17 is a rear perspective view on the plane side according to the second embodiment. FIG. 18 is a right side view according to the second embodiment. FIG. 19 is a left side view according to the second embodiment. FIG. 20 is a plan view according to the second embodiment. FIG. 21 is a bottom view according to the second embodiment. FIG. 22 is a front view according to the second embodiment. FIG. 23 is a rear view according to the second embodiment. FIG. 24 is a rear perspective view of the bottom surface according to the second embodiment. FIG. 25 is a front perspective view on the bottom surface side according to the second embodiment. FIG. 26 is an enlarged CC sectional view of the DD and EE portions according to the second embodiment. FIG. 27 is an enlarged BB cross-sectional view of the DD portion according to the second embodiment. FIG. 28 is a cross-sectional view taken along the line AA according to the second embodiment. FIG. 29 is a diagram showing the shape of the surface of the bottom wall portion on the side of the internal space according to the second embodiment.

The only difference between the second embodiment and the first embodiment is the configuration of the bottom wall portion 1A1 (the configuration of the surface 1A1f), and the others are the first embodiment and the second embodiment. Has the same configuration. In the second embodiment, as shown in FIGS. 21, 24, 25, 28, and 29, the surface 1A1f of the bottom wall portion 1A1 has three surface regions (surface region PE2a, surface region PE2b, surface region). It can be classified into PE2c). In the second embodiment, the surface 1A1f includes a surface region PE2a, a surface region PE2b, and a surface region PE2c. Each of the surface region PE2a, the surface region PE2b, and the surface region PE2c is flat and can be inclined so as to approach the ceiling wall portion 1A2 from the side of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3. The surface 1A1f can also be divided into a plurality of four or more surface regions. By partitioning the surface 1A1f into multiple surfaces, a large liquid storage volume of the main space SP1 can be secured. The region of the surface 1A1f of the bottom wall portion 1A1 that is farthest from the ceiling wall portion 1A2 (the lowest region of the surface 1A1f of the bottom wall portion 1A1 when the cell storage container 1 is laid flat) and supply / discharge. As long as it is connected to the parts 1A5, it is not limited to the illustrated embodiment and can be deformed. The surface 1A1f can also be a curved surface. In this case, the curved surface is inclined so as to approach the ceiling wall portion 1A2 from the side of the supply / discharge portion 1A5 toward the side of the side wall portion 1A3. The configuration shown in FIG. 15 is also applied in the second embodiment.

The first embodiment will be comprehensively described with reference to FIGS. 30, 31, 32, and 33. FIG. 30 is a rear perspective view on the reference bottom side according to the first embodiment. FIG. 31 is a reference enlarged CC sectional view in the DD and EE portions according to the first embodiment. FIG. 32 is a reference enlarged BB cross-sectional view in the DD portion according to the first embodiment. FIG. 33 is a cross-sectional view taken along the line AA according to the first embodiment. The second embodiment will be comprehensively described with reference to FIGS. 34, 35, 36, and 37. FIG. 34 is a rear perspective view of the reference bottom surface side according to the second embodiment. FIG. 35 is a reference enlarged CC sectional view in the DD and EE portions according to the second embodiment. FIG. 36 is a reference enlarged BB cross-sectional view in the DD portion according to the second embodiment. FIG. 37 is a cross-sectional view taken along the line AA according to the second embodiment.

Description of Articles Related to Design: This article (cell storage container 1 according to one embodiment, the same applies hereinafter) is a cell storage container for collecting cells cultured in a cell culture container. In this article, the air pressure is regulated by the aeration part, so that the medium, the culture solution, and the cell suspension are supplied and discharged through the supply and discharge part. In this article, not only the partition wall but also the ceiling wall is provided with protrusions to further reduce the gap and maintain the function of adjusting the air pressure in the ventilation part, while ventilating the medium, culture solution, and cell suspension. The inflow to the part can be suppressed. In this article, the upper surface portion of the bottom wall portion is divided into a plurality of surface regions, and is inclined downward from the back side wall portion toward the supply / discharge portion side. Due to the inclination of the upper surface of the bottom wall of the article, the contained medium, culture medium, and cell suspension can be discharged from the supply / discharge section while the article is placed on a horizontal plane.

Description of design: This article is entirely transparent.

Although the principles of the invention have been illustrated and described above in preferred embodiments, it will be appreciated by those skilled in the art that the invention can be modified in arrangement and detail without departing from such principles. The present invention is not limited to the specific configuration disclosed in the present embodiment. Therefore, we claim all amendments and changes that come from the claims and their spiritual scope.

1 ... Cell storage container, 1A ... Storage part, 1A1 ... Bottom wall part, 1A1f ... Surface, 1A2 ... Ceiling wall part, 1A3 ... Side wall part, 1A4 ... Partition part, 1A4a ... End side part, 1A4b ... End side part, 1A4c ... end side, 1A4d ... end side, 1A4f ... surface, 1A5 ... supply / discharge part, 1A5a ... opening, 1A5b ... opening, 1A6 ... ventilation part, 1A6a ... opening, 1A6b ... opening, 1A7 ... protrusion, 1B ... frame Part, 1B1 ... Bottom, 1B2 ... Wall, ER ... Isolation area, PE1a ... Surface area, PE1b ... Surface area, PE2a ... Surface area, PE2b ... Surface area, PE2c ... Surface area, SP ... Internal space, SP1 ... Main space , VD1 ... Gap area, VD2 ... Gap area.

Claims (5)

It is a cell storage container
It has a bottom wall part, a ceiling wall part, a side wall part, a partition wall part, a supply / discharge part, and a ventilation part.
The bottom wall portion, the ceiling wall portion, and the side wall portion define the internal space of the cell storage container.
The bottom wall portion and the ceiling wall portion are provided so as to be separated from each other via the side wall portion.
The side wall portion extends from the bottom wall portion to the ceiling wall portion.
The supply / discharge portion is provided on the side wall portion, communicates the internal space with the space outside the cell storage container, and is arranged on the side of the bottom wall portion.
The surface of the bottom wall portion faces the internal space and is connected to the inner surface of the supply / discharge portion.
The ventilation portion is provided on the side wall portion, and communicates the internal space with the space outside the cell storage container.
The partition wall portion extends from the bottom wall portion toward the ceiling wall portion in the internal space, and defines an isolation region in front of the ventilation portion so as to cover the ventilation portion.
The two ends of the partition wall extending from the bottom wall portion toward the ceiling wall portion are joined to the side wall portion.
An opening of the ventilation portion on the side of the isolation region is arranged between the two ends of the partition wall portion.
A first gap region is provided between the partition wall portion and the ceiling wall portion.
The first gap region communicates the inside of the isolation region with the outside of the isolation region in the interior space.
Cell storage container. With more protrusions
The protrusion extends from the ceiling wall portion toward the bottom wall portion in the internal space, extends along the partition wall portion outside the isolation region in the internal space, and is a first gap region. It is provided to cover the
A second gap region is provided between the protrusion and the partition wall.
The cell storage container according to claim 1. The surface of the bottom wall portion is inclined so as to approach the ceiling wall portion from the side of the supply / discharge portion toward the side of the side wall portion.
The cell containing container according to claim 1 or 2. The surface of the bottom wall portion is divided into a plurality of surface areas.
Each of the plurality of surface regions is flat and is inclined so as to approach the ceiling wall portion from the side of the supply / discharge portion toward the side of the side wall portion.
The cell storage container according to claim 3. The surface of the bottom wall portion is a curved surface.
The cell storage container according to claim 3.

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