JP6937062B2 - An oral composition that utilizes Itea containing in vivo functional ingredients in the form of a plant. - Google Patents

Description

The present invention is an oral composition that utilizes Itea containing an in vivo functional component in the form of a plant, more specifically, an oral composition having a function of suppressing body fat accumulation and / or a function of suppressing increase in total cholesterol level. Regarding.
Itea or clethra barbiner (Itea is called clethra barbiner in some regions. In the present invention, clethra barbiner = clethra barbiner; hereinafter, this description is omitted) and / or total cholesterol. It uses the price increase suppression function.

Due to growing public interest in health, attempts to maintain health through daily food intake are attracting attention. In particular, the development of dietary fiber-containing food materials containing in vivo functional ingredients is remarkable. It has often been practiced to dry plants such as leaves and stems into powder to make tea and food materials, or to powder plants having medicinal properties and use them in health foods. For example, a method of cutting the leaves and stems of Moroheiya into appropriate dimensions, steaming them with raw steam for several minutes, then drying them while kneading and loosening them, and then crushing them into Moroheiya powder (Patent Document 1), GABA having a blood pressure lowering effect. A method (Patent Document 2) is known in which green leaves of Gramineae plants having a high concentration of Nalta jute are treated with a microwave and then dried to obtain a dry powder.

In drying by heating mainly with hot air, the dry powder discolors to brown etc. and looks bad, and the components are often denatured by heat, the content is reduced or disappeared, etc., so the freeze-drying method is adopted. Often done. For example, a succulent plant is sterilized and washed using sodium hypochlorite in a solid state, and when vacuum freeze-dried, it is dried at a low temperature for a long time. (Patent Document 3), plants belonging to the genus Wild celery or Kawaraboufu of the family Seri, raw and any of the plants such as Touki, Inutouki or Hyugatouki, and so-called mountain ginseng. A method for producing a raw material that freeze-drys sun-dried leaves, stems, and seeds into powder (Patent Document 4), and adds excipients such as starch and a thickener while the okura is frozen-dried as it is. It is known to produce dry powder such as a method for producing okura tea by making it into a tablet or by mixing it with a substance such as celery or herb tea (Patent Document 5).

Japanese Unexamined Patent Publication No. 2003-18975 Japanese Unexamined Patent Publication No. 2002-58446 JP-A-2009-284891 Japanese Unexamined Patent Publication No. 2008-303174 Japanese Unexamined Patent Publication No. 2006-61167 Japanese Patent No. 6437721

Abstracts of the Society for Biotechnology, Japan 2007, 108, 2007-08-02 Phytochemistry 48 (2): 241-248 Biosci.Biotechnol.Biochem.2006,70 (9), 2081-2085 Life Sci. 2007,81 (7), 592-599 Journal of Japanese Society of Nutrition and Food Science Vol. 59 (2006) No. 2 P119-121 Journal of the Japanese Society of Food Science and Engineering Vol. 57 (2010) No. 6 P263-267

With the spread of preventive medicine knowledge, attempts are being made to maintain health by ingesting naturally derived foods that have preventive physiological activity in a healthy or semi-healthy state before getting sick, and natural materials. Is also marketed as a health food. Many of them advocate anti-diabetic effects, but the reality is that few have been specifically proven to be effective. As far as the present inventors know, rare sugar is a harmless natural product that can be added to the original food, and foods and drinks that do not affect the taste and have a safe natural product itself have not yet been developed. .. That is, no report has yet been verified that the plant itself, which does not correspond to an extract from a natural product, has a characteristic physiological activity that improves one or more of blood glucose level, weight gain, and visceral fat accumulation. It is considered that there is a strong social demand for a safe natural product itself, which has excellent characteristic physiological activity to improve blood sugar level, weight gain, and one or more of visceral fat accumulation. According to the present invention, plants such as leaves and stems of Zuina or Ryobu contain rare sugars having physiological activity, and the rare sugars improve blood glucose level, weight gain, and one or more of visceral fat accumulation. Based on the characteristic bioactive substances, we are trying to examine whether these plants themselves have the same effect as rare sugars.
Deciduous shrubs of the genus Itea of the family Saxifragaceae used new leaves as food, but there are no reports of nutritional studies. Therefore, first, we feed rats with a diet containing dry powder of Itea, collect basic data on the physiological action of Itea, and see if it has the same effect as D-allulose (= D-psicose). The purpose was to examine. Next, in order to elucidate the components of Itea in more detail, the rare sugars D-allulose and alitol (alitol is a rare sugar of sugar alcohol, so it may be referred to as "sugar alcohol rare sugar"). In particular, we decided to examine the effect of suppressing body fat accumulation and cholesterol metabolism.

According to the present invention, the body fat accumulation of the natural plant itself, that is, the plant itself that produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in the natural world. It is an object of the present invention to provide an oral composition that utilizes an inhibitory function and / or an inhibitory function of increasing total cholesterol level.

It is known that plants such as leaves and stems of Itea or Clethra barbiner (hereinafter, may be abbreviated as "Itea") contain rare sugars having physiological activity, and contain rare sugars. We examined the body fat accumulation inhibitory effect and cholesterol metabolism of Itea so that Itea can be used in the plant form, and the body fat accumulation inhibitory function containing Itea in the plant form and / or total cholesterol. We have invented an oral composition having a function of suppressing an increase in value and a healthy food and drink containing the same.

The gist of the present invention is the following (1) to ( 11 ) body fat accumulation inhibitor or total cholesterol elevation inhibitor.
(1) The untreated plant itself, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, physically, chemically or treated themselves obtained by treating biologically or, characterized by containing as an active ingredient an extract itself plant comprising a D- allose and Arito Le, the plant is a Zuina or both parts, A body fat accumulation inhibitor or a total cholesterol level increase inhibitor.
( 2 ) The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to (1) above, wherein the plant is a plant that has been treated to increase the rare sugar content.
( 3 ) The plant is an adult tree, an adult tree subjected to D-allulose-increasing treatment, and / or a bud, a leaf, a branch, a flower or a root of a mutant such as a multiple of any of them, or a germinated seed. The body fat accumulation inhibitor according to any one of (1) or (2) above, or the total cholesterol level increase inhibitor, which is an entire plant grown by subculture or tissue culture.
( 4 ) The body fat accumulation inhibitor according to any one of (1) to (3 ) above, or the total cholesterol level increase inhibitor, wherein the plant is a dried product.
( 5 ) The dried product is a freeze-dried product, which is the body fat accumulation inhibitor or the total cholesterol level increase inhibitor according to (4) above.
( 6 ) The freeze-dried product according to (5 ) above, which is a freeze-dried product obtained by a freeze-drying device for a liquid nitrogen-immersed frozen product, or an agent for suppressing an increase in total cholesterol level.
( 7 ) The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to (5 ) or ( 6 ) above, wherein the freeze-dried product is a crushed or crushed product of the freeze-dried product.
( 8 ) The crushed or crushed product is a powdery substance, the body fat accumulation inhibitor according to (7 ) above, or the total cholesterol level increase inhibitor.
( 9 ) The body fat accumulation inhibitor according to any one of (1) to (3 ) above, wherein the extract of the plant is a water extract or an alcohol extract of the aqueous medium extract residue of the plant. Or, a total cholesterol level increase inhibitor.
( 10 ) The body fat accumulation inhibitor according to any one of (1) to (9 ) above, or total cholesterol, in which the plant body or the extract of the plant body is in the form of powder, paste or solution. Price increase inhibitor.
( 11 ) The body fat accumulation inhibitor or the total cholesterol level increase inhibitor according to any one of (1) to (10 ) above, which is orally administered.

The gist of the present invention is the following oral compositions (12 ) to ( 33).
( 12 ) The untreated plant itself, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature, physically, chemically or treated themselves obtained by treating biologically, and / or, characterized in that an active ingredient an extract itself plant comprising a D- allose and Arito Le, the plant is a Zuina or both parts , An oral composition having a function of suppressing body fat accumulation and / or a function of suppressing increase in total cholesterol level.
( 13 ) The oral composition according to (12 ) above, wherein the plant is a plant that has been treated to increase the rare sugar content.
( 14 ) The plant is a bud, leaf, branch, flower or root, or germinated seed of a mutant such as an adult tree, an adult tree subjected to D-allulose-increasing treatment, and / or a multiple of any of them. The oral composition according to (12) or (13) above, which is an entire plant grown by subculture or tissue culture.

( 15 ) The oral composition according to any one of (12 ) to ( 14 ) above, wherein the plant is a dried product.
( 16 ) The oral composition according to (15 ) above, wherein the dried product is a freeze-dried product.
( 17 ) The oral composition according to (16 ) above, wherein the freeze-dried product is a freeze-dried product obtained by a freeze-drying apparatus for a liquid nitrogen-immersed frozen body.
( 18 ) The oral composition according to (16 ) or ( 17 ) above, wherein the lyophilized product is a crushed or crushed product of the lyophilized product.
( 19 ) The oral composition according to (18 ) above, wherein the crushed or crushed product is a powder.
( 20 ) The oral composition according to any one of (12 ) to ( 14 ) above, wherein the extract of the plant is a water extract or an alcohol extract of the residue of the aqueous medium extract of the plant.
( 21 ) The oral composition according to any one of (12 ) to ( 14 ) above, wherein the plant or an extract of the plant is in the form of powder, paste or solution.

( 22 ) The oral composition according to any one of (12 ) to ( 21 ) above, further comprising a dietary fiber-containing material.
( 23 ) The oral composition according to (22 ) above, wherein the dietary fiber-containing material is a tea powder selected from Japanese tea, black tea and Chinese tea.
( 24 ) The oral composition according to (22 ) above, wherein the dietary fiber-containing material is a powder of a green plant.
( 25 ) The dietary fiber-containing material is one or more of Angelica keiskei, young wheat leaves, green tea, kale, young kansho leaves, broccoli, moroheiya, young sesame leaves, mulberry leaves, button siler, and yomogi, as described in ( 22 ) above. Oral composition.

( 26 ) The oral composition according to any one of (12 ) to ( 25 ) above, which is a food, quasi-drug or pharmaceutical product.
( 27 ) The oral composition according to (26 ) above, which is used as a health food, a health drink or a pharmaceutical product for suppressing body fat accumulation.
( 28 ) The oral composition according to (26 ) above, which is used as a health food, a health drink or a pharmaceutical product for suppressing an increase in total cholesterol level.
( 29 ) The above, wherein the food is prepared as a supplement, a functional food, a health food, an enteric nutrition food, a special purpose food, a health supplement, a food for specified health use, or a food with a nutritional function. The oral composition according to any one of ( 26 ) to ( 28).
( 30 ) The oral composition according to (29 ) above, wherein the special purpose food is a food for the sick.
( 31 ) Any of the above (12 ) to ( 30 ), which is prepared in the form of tablets, rounds, capsules, powders, granules, fine granules, troches, or liquids. The oral composition according to.
( 32 ) The oral composition according to (31 ) above, which is a granule or a tablet.
( 33 ) The food is a beverage, confectionery, cold confectionery, noodles, processed marine food, processed livestock food, dairy product, fat and oil processed food, seasoning, enteral nutrition food, soup, stew, prepared food, pickles, or The oral composition according to any one of (26 ) to ( 32 ) above, which is prepared as a bread.

Plants such as Zuina leaves and stems contain bioactive rare sugars, which are characteristic bioactive substances that improve blood sugar levels, weight gain, and one or more of visceral fat accumulation. Based on the above, it was expected that Zuina would have such bioactivity even in the form of a plant, but its verification has not yet been made. As a result of collecting basic data on the physiological action of Itea by feeding rats with a diet containing dry powder of Itea, the effect of the rare sugar D-allulose on the effect of suppressing body fat accumulation and the suppression of total cholesterol level increase It was also found that the influence of the components in Itea was greater. In addition, under high-fat, high-sucrose dietary conditions, the anti-obesity effect of Itea dry powder was verified, and whether the anti-obesity effect was due to the rare sugars (D-allulose and alitol) in the Itea dry powder, and dried with Itea. The anti-obesity effect of the powder was confirmed, and the synergistic effect of D-allose and alitol was confirmed. Plants or plant extracts that produce and contain the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature, suppress body fat accumulation or total cholesterol. Expected to have an effect of suppressing price increases.

According to the present invention, the plant itself, which is a natural product, that is, the plant itself or an extract thereof, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, in the plant itself. , An agent or an oral composition that utilizes the function of suppressing the accumulation of body fat and / or the function of suppressing the increase in total cholesterol level can be provided.

It is a figure which shows the tissue and the inspection item collected in Example 1. FIG. It is a graph which shows the body weight transition of the rat of each diet group in Example 1. It is a graph which shows the body weight and the food intake of the rat of each diet group in Example 1. FIG. It is a graph which shows the relative weight of the heart, the liver, the kidney, and the spleen of the rat of each diet group in Example 1. FIG. It is a graph which shows the relative weight of the perirenal adipose tissue, the accessory testicle adipose tissue, the mesenteric adipose tissue, and the total intraperitoneal adipose tissue of the rat of each diet group in Example 1. It is a graph which shows total protein, lipase, total cholesterol, and crawl among the serum components of rats of each diet group in Example 1. It is a graph which shows glycogen, total fat, cholesterol, and triglyceride among the liver components of the rat of each diet group in Example 1. It is a graph which shows the carcass fat mass, the carcass fat percentage, the total body fat mass, and the total body fat percentage of the rat of each diet group in Example 1. It is a figure which shows the tissue and the inspection item collected in Example 2. FIG. It is a graph which shows the body weight and the food intake of the rat of each diet group in Example 2. It is a graph which shows the relative weight of the heart, the liver, the kidney, and the spleen of the rat of each diet group in Example 2. FIG. It is a graph which shows the relative weight of the epididymal fat, the perirenal fat, the mesenteric fat, and the total intraperitoneal fat of the rat of each diet group in Example 2. It is a graph which shows the carcass fat mass, the carcass fat percentage, the total body fat mass, and the total body fat percentage of the rat of each diet group in Example 2. It is a graph which shows the concentration of glucose, insulin and triglyceride which are serum components of the rat of each diet group in Example 2. FIG. 3 is a graph showing the concentrations of total cholesterol, HDL cholesterol, and free fatty acids, which are serum components of rats in each diet group in Example 2. Various substances in Zuna [1-naphthalene acetic acid (abbreviated as NNA), 2,4-dichlorophenoxyacetic acid (abbreviated as 2,4-D), 6-benzylaminopurine (abbreviated as BAP), sodium chloride (NaCl), D-glucose , Sucrose, D-fructose, D-allose] to examine whether the D-allose content increases. In the figure, each processing concentration is shown on the horizontal axis.

[Plants containing rare sugars]
A plant of the genus Itea is a plant that produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature. That is, it is the only plant that produces and contains D-allulose and alitol, which have a bioactive action called rare sugar in nature, in the plant body. Plants of the genus Itea are sold as seedlings in Japan, and those belonging to some species are grown in ornamental gardens due to their long spikes and fragrant flower heads, and can also be used as hedges or ikebana. It is used as. Although it is sold as a seedling in Japan, it could not be propagated by seed germination. It was found that plants of the genus Itea produce and contain D-allulose and alitol, which have bioactive effects, in the plant body, and the development of a method for efficiently germinating seeds is eagerly desired. became.

The plants of the present invention include buds, leaves, branches, flowers or roots, or germinated seeds of mature trees, mature trees subjected to D-allulose-increasing treatment, and / or variants of any of them. The whole plant grown by subculture or tissue culture. More specifically, for example, plants such as wild and polymorphic / mutant plants, plants obtained by cultivation or plants obtained by culture such as tissue culture, for example, leaves, flowers, branches, stems, fruits, etc. Roots, seeds, cultured cells or organs, callus and the like can be mentioned, and various processed products obtained by treating these as they are or by physically, chemically or biologically treating them can be mentioned.
Examples of the physical / chemical treatment method include drying treatments such as sun-drying, air-drying, and freeze-drying, and crushing treatments using a blender, homogenizer, ball mill, etc. Examples include freeze-dried products and crushed products. Examples of the biological treatment method include a fermentation method and the like, and examples of the biological treatment product include a fermentation treatment product.

In plants of the genus Itea, it is efficient to cultivate a plant cut from a Itea plant obtained by sexually breeding (seed breeding) a wild species of Itea, regardless of the plant tissue culture method, in a plant culture medium. A method for producing cloned seedlings in a good and economical manner has been developed (Patent Document 6).
It is preferred to use mature tree buds, leaves, branches, flowers or roots, or germinated seeds, plants grown by subculture or tissue culture.

The only plant containing the rare sugar D-allulose is the genus Itea (Non-Patent Document 1). In addition, D-allulose is known to inhibit the growth of many plants, and is also known to have an insulin secretory action (Non-Patent Document 2) and an action to prevent arteriosclerosis (Non-Patent Document 3). .. It has about 70% sweetness of sugar, but has almost no calories. In animal experiments with rats, "slowing the rise in blood glucose level after meals" (Non-Patent Document 4), "suppressing the accumulation of visceral fat" (Non-Patent Document 5), "less likely to cause arteriosclerosis" (Non-Patent Document 6) ) And other research results have been reported. However, it has been reported that since D-allulose is reduced by cooking, the effect of suppressing the increase in blood glucose level after meals is affected, and the effect is reduced as compared with the non-heated state. For those cooked at high temperatures, such as karinto, the effect is greatly reduced. It has been confirmed to have an effect of inhibiting the growth of nematodes, and may be used as an anthelmintic. The present invention relates to a rare sugar-containing plant powder produced by freeze-drying and crushing a plant of Itea or Clethra barbiner. Rare sugars contained in these plants can be easily used, and powders that can be used as raw materials for foods or health-promoting agents can be obtained by forming them into a form that can be widely applied. Is. The present invention has never been verified that a rare sugar containing sucrose even in the form of a plant has a characteristic physiological activity that improves one or more of blood glucose level, weight gain, and visceral fat accumulation. For the first time, the anti-obesity effect of Zuina dry powder was verified under high-fat, high-sucrose dietary conditions. The anti-obesity effect was also due to the rare sugar (D-allulose) and alitol in the dry powder of Itea, and the synergistic effect of D-allulose and alitol could be confirmed. Plants or plant extracts that produce and contain the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature, suppress body fat accumulation or total cholesterol. It is expected to have the effect of suppressing price increases.

[Plants treated to increase the rare sugar content]
In order to produce plants that have been treated to increase the rare sugar content, we have found that various substances in Zuina [1-naphthalene acetate (abbreviated as NNA), a plant growth modifier exhibiting auxin-like activity, 2 , 4-Dichlorophenoxyacetic acid (abbreviation 2,4-D) A plant hormone agent with auxin-like action, 6-benzylaminopurine (abbreviation BAP) First-generation synthetic cytokinin that stimulates plant growth, sodium chloride (NaCl) ), D-glucose, sucrose, D-fractose, D-allose], and examined whether the D-allulose content increased.
Cultured individuals about 2 months after passage were used and tested in a ^{growth chamber (normal light conditions; 100-150 μmоlm-2} S- ^1). Hydroponics was carried out for 3 days and each treatment was carried out for 10 days under the condition of 28 ° C. In hydroponics, the cultured plant was floated in pure water, and the sugar treatment was carried out by exchanging the sugar solution every two days. The sampled leaves were frozen under liquid nitrogen, crushed into powder, and then extracted with sterile water. Dried baker's yeast was added to the above sugar extract to remove D-fructose and D-glucose. The amount of D-allulose in this rare sugar solution was quantified using the cysteine-carbazole sulfate method. The results are shown in FIG. In the figure, each processing concentration is indicated on the horizontal axis. As shown in FIG. 16, it was clarified that the amount of D-allulose in Itea fluctuated greatly by performing various treatments. There are some that decrease by pretreatment and some that increase by conversely. In particular, the fact that D-allulose in Itea can be increased by performing pretreatment that can increase the D-allulose content of plants by sugar treatment is extremely valuable.

表中のデータ値はシステイン・カルバゾール硫酸法を用いて定量したD-アルロース含量を明条件での未処理区を１００％とした相対値（相対値（％））と、明・暗各条件下での未処理区を１００％とした相対値（相対値 (%)<各未処理比>）で示した。Based on the above results, the D-allulose content of leaves by sugar (monosaccharide / disaccharide) treatment under light and dark conditions was experimentally investigated, and the results are shown in Table 1. The data values in Table 1 are relative values with the D-allulose content quantified using the cysteine-carbazole sulfuric acid method as 100% in the untreated group under light conditions (the untreated ratio is 100% in the untreated group under dark conditions). Indicated. The experiment was conducted in a growth chamber (100-150 μmоlm- ² S- ¹ ) using cultured individuals about 2 months after passage. Hydroponics was carried out for 3 days (normal light) and sugar treatment was carried out for 2 days (normal light condition / completely shaded dark condition) under the condition of 28 ° C. In hydroponics, the cultured plant was floated in pure water and sampled on the 3rd day after the start of sugar treatment (unlike the above, no sugar solution exchange was performed). The sampled leaves were frozen under liquid nitrogen, crushed into powder, and then extracted with sterile water. Dried baker's yeast was added to the above sugar extract to remove D-fructose and D-glucose. The amount of D-allulose in this rare sugar solution was quantified using the cysteine-carbazole sulfate method. Each sugar was treated to 10 mM.

The data values in the table are relative values (relative value (%)) with the D-allulose content quantified using the cysteine-carbazole sulfuric acid method as 100% in the untreated group under bright conditions, and light and dark conditions. It is shown as a relative value (relative value (%) <each untreated ratio>) with the untreated section in (1) as 100%.

In addition, it was confirmed by experiments that the contents of D-allulose and alitol can be increased by artificially multiplying the chromosomes.
In the experiment, the sugar content of leaves in diploid and tetraploid under light and dark conditions was examined, and the results are shown in Table 2. The data values in Table 2 are all sugar contents (mg / g dry weight) per 1 g of dry weight of leaves.
The D-psicose content was shown as a relative value with the untreated group under light conditions as 100% (the untreated ratio was 100% for the untreated group under dark conditions). A diploid Itea subcultured individual was treated with 125 μM amiprophosmethyl (APM) for 24 hours, and the obtained individual was subcultured and polyploidy analysis was performed using flow cytometry. It was unified to produce a tetraploid individual. In addition to amiprophosmethyl, treatment with colchicine, for example, can be considered for chromosome doubling.
Cultured individuals about 2 months after passage were used and tested in a ^{growth chamber (100-150 μmоlm-2} S- ^1). Hydroponics was carried out for 3 days (normal light) and light and dark treatment was carried out for 3 days (normal light condition / complete shading dark condition) under the condition of 28 ° C. The leaves were sampled, freeze-dried for 2 days, weighed, and then the monosaccharides contained were measured. The sugar content was calculated from the HPLC peak area and the calibration curve of the standard sample. Experiments are performed with n = 4 for diploids under bright conditions and n = 5 for other cases.

Examples of the plant extract include extracts obtained from the above-mentioned plants by various extraction methods. Examples of the extraction method include extraction of various solvents and extraction of supercritical fluid. Extracts are prepared by various solid-liquid separation methods such as sedimentation separation, cake filtration, clarification filtration, centrifugal filtration, centrifugal sedimentation, squeezing separation, filter press, various concentration methods, various drying methods, and formulation methods such as granulation or powdering. It may be treated by various purification methods and the like. Examples of the purification method include a solvent fractionation method, a column chromatograph method, a recrystallization method and the like. Concentration and drying methods include freeze-drying, natural drying, hot air drying, ventilation drying, blast drying, spray drying, vacuum drying, sun drying, vacuum drying, fluidized layer drying, foam layer drying, drum dryer and other film drying methods. , Ultrasonic drying, electromagnetic wave drying and other drying methods, preferably spray drying method and freeze drying method.
For the extraction and the treatment of the extract, for example, an antioxidant, a preservative and the like can be added. As the solvent used for solvent extraction, any solvent can be used as long as it can extract the functional components of the plant, for example, water, distilled water, deionized water, an aqueous inorganic salt solution, an aqueous medium such as a buffer, methanol, ethanol, etc. Monohydric alcohols such as propanol and butanol, polyhydric alcohols such as propylene glycol and glycerol, hexane, toluene, petroleum ether, benzene, ethyl acetate, chloroform, dichloromethane, 1,1,2-trichloroethane, dimethylsulfoxide, acetone Examples thereof include organic solvents such as, and aqueous media and alcohols are preferable.
Examples of the buffer solution include a phosphate buffer solution and a citric acid buffer solution. Examples of the inorganic salt in the aqueous solution of the inorganic salt include sodium chloride, potassium chloride, calcium chloride and the like. The alcohol is preferably a monohydric alcohol, and the monohydric alcohol is preferably ethanol.
These solvents can be used alone or in admixture. As the mixed solvent, a hydrous alcohol is preferable, a hydrous monohydric alcohol is more preferable, and a hydrous ethanol is particularly preferable. The water content is preferably 70% or less, more preferably 40% or less. As the solvent, carbon dioxide that has been supercritical fluidized can also be used.
Extraction is carried out using, for example, 0.1 part by weight to 10,000 parts by weight of the solvent, preferably 1 part by weight to 100 parts by weight, based on 1 part by weight of the plant. The extraction temperature is not particularly limited, but is preferably 0 ° C to 100 ° C, more preferably 20 ° C to 90 ° C. The extraction time is not particularly limited, but is preferably 1 minute to 1 week, more preferably 30 minutes to 1 day. Further, as an extraction method from Zina or Ryobu, the residue obtained by extracting the above-mentioned Zina or Ryobu, the above-mentioned physically or chemically treated product of Zina or Ryobu, or the above-mentioned biologically treated product in an aqueous medium is water, alcohol, or water-containing. It can be extracted with alcohol.
The aqueous medium is not particularly limited, but water, pure water, and deionized water are preferable. The extraction temperature when extracting with an aqueous medium and alcohol or hydrous alcohol is not particularly limited, but is preferably 0 ° C to 100 ° C, more preferably 20 ° C to 90 ° C. The extraction time is not particularly limited, but is preferably 1 minute to 1 week, more preferably 30 minutes to 1 day. Further, as the Itea or Clethra barbiner, it is preferable to use one that has been dried or fermented.
The equipment used for extraction is not particularly limited, and examples thereof include a container, a stirrer, a reflux condenser, a Soxhlet extractor, a homogenizer, a shaker, an ultrasonic generator, etc. devised for efficient extraction. The liver function protective agent or improving agent of the present invention contains Itea or clethra barbiner prepared by the above-mentioned method or an extract thereof, and if necessary, one or more pharmacologically acceptable carriers, and further, if necessary. It may contain active ingredients for other treatments, as appropriate.

For example, it is known that the leaves of Itea contain about 5% of the raw weight of D-allulose among rare sugars. In the present invention, for the purpose of utilizing the rare sugar contained in these plant objects, the rare sugar is contained by powdering the plant body including the leaves, buds, stems, roots, etc. of Zuina. It can be used as a powder. For the collection of plants from Zuina or Ryobu, for example, the collection of leaves from adult trees or mature trees subjected to D-allulose-increasing treatment and variants such as multiples thereof, or seedlings germinated from seeds, tissue culture Alternatively, a powder can be obtained by collecting the entire plant including the leaves, stems and roots of the subcultured seedlings and lyophilizing them.
In the present invention, commercially available freeze-drying equipment is used to freeze-dry the Itea plant. Compared to other drying methods, the freeze-drying method is a low-temperature treatment, so there is no nutrient destruction and excellent resilience. It is also a feature of this drying method that it prevents tissue destruction due to dehydration. Since nearly 90% of food is water, great care must be taken against spoilage. Freeze-drying is used in the production of preserved foods because the final degree of drying is much better than other drying methods. In addition, the weight of dried food is reduced by the amount of water removed, so it is also suitable for transportation. The powder of the Itea or Clethra barbiner plant is produced from the Itea or Clethra barbiner plant without impairing the functional components of the Itea or Clethra barbiner plant as much as possible. For example, a step of crushing a plant of Zuina or Ryobu to make a slurry, a step of pre-freezing the slurry into a subdivided freezing mold, and a vacuum freeze-drying treatment of reducing the frozen product to a water content of 3 to 5% are performed. It is characterized in that it is composed of a step and a step of preparing a powder of the dried product obtained from the step. Further, as the drying treatment, vacuum drying and spray drying are applied.

The cultivation and freeze-drying of Itea will be described in detail below as an example.
Currently, Itea and Clethra barbiner are known as plants containing rare sugars, and the following is described according to a botanical pictorial book or the like.
[Itea] Itea is a plant belonging to the genus Itea. The genus Itea (genus Itea, genus Itea, scientific name: Itea spp.) Is a deciduous shrub of the family Yukinoshita, and is a genus of plants consisting of about 10 species of shrubs. The young leaves are boiled and eaten like yomena, so they are also called yomenanoki. There are saw teeth. Approximately 8 pairs of parallel lateral veins stand out on the leaf surface. From May to June, the tips of the branches are borne with upward racemes 5 to 17 cm in length, and many white florets open. The petals are ovate-lanceolate, with 5 upright, sepals and stamens with the same number as petals, 1 pistil, stigma head-shaped, and ovary half-upper. Itea grows in the warm mountains and is distributed in the southern part of the Kinki region, Shikoku, and Kyushu. The genus Itea is known to be the only plant that produces D-allulose and alitol contained in rare sugars in the plant body. It is also sold as a seedling in Japan.

[Clethra]
Clethra barbiner is a plant belonging to the family Clethraceae, and is explained in the botanical picture book as follows.
Distributed in Hokkaido, Honshu, Shikoku, Kyushu and Jeju Island on the Korean Peninsula, it is a small tree with deciduous leaves that grows in the forest. The shoots have stellate hair. The leaves are pedunculated and alternate, with wide inverted needles, serrated edges, gathering at the tips of the branches, sparsely surface hairless or stellate hairs, and densely haired on the back veins. In summer, racemes with a length of 6 to 15 cm are borne on the tips of the branches, and small white flowers are densely attached. The gaku is small and splits into five. The corolla is 5 to 6 mm in diameter, has 5 deep splits, and resembles a small plum blossom. There are 10 stamens and 1 pistil. Spherical and small fruit. The wood will be fine charcoal and the shoots will be edible. Ryobu does not contain the rare sugar allulose, but depending on the region, Itea may be called Ryobu. The present invention relates to Itea.

[Cultivation from Itea seeds]
Since the seeds of Itea are dormant, they will not germinate even if they are sown unless the dormancy is broken. In order to break the dormancy of seeds, it is necessary to store them in a cool and dark place in a dry atmosphere for a predetermined period of time. It is done by storing for a period of time. As a drying condition, it is preferable that the water vapor pressure is low, and for example, it is stored together with a large amount of silica gel in a closed plastic container. The storage temperature is 4 ° C. or lower, preferably 4 ° C. or lower. The storage period under these conditions is preferably 2 weeks or longer, preferably 2 to 6 months. Since Itea seeds are light germinated seeds, they are difficult to germinate when covered with soil, and germination is preferably performed under a temperature condition of 25 to 30 ° C in a bright place, and the germination rate after 2 weeks at 28 ° C is about 60%. Is.
To grow germinated seeds, for example, the germinated seeds can be grown in the same 1/2 MS medium [containing 1% sucrose (pH 5.8)] or planted in rock wool or soil in the same manner as normal vegetation. Can grow. Whether the germinated seeds are planted in the medium or in soil, the subsequent growth speed is comparable, but soil cultivation is slightly more wooded. Compared to these, rock wool cultivation grows faster in the early stages of cultivation. The germinated seeds can be transplanted, for example, about 10 days after sowing.

[Growth by tissue culture]
1/2 MS medium (1% sucrose) as it is, or as it is, the seedlings in which the 2nd to 5th leaves of Itea are developed, or the Itea plant containing the stem apex or stem of a normal Itea sapling, or as it is after removing the leaves. There is a cultivation method of Itea that enables a large amount of seedlings to grow by simply placing it in a plant culture medium such as (added or not added) or by culturing it by thrusting it like a cutting. As the Itea plant, seedlings obtained by breaking the dormancy of seeds improved by crossing can be used. It is possible to easily obtain a large amount of Itea plants or seedlings by sprouting seeds that have broken dormancy and mass-producing seedlings by subculture thereof.

[Growth of Itea seedlings by subculture]
Mass production of Itea seedlings can be carried out by tissue culture in which a part of the plant is taken out and aseptically cultured in a test tube or the like. For example, a large amount of seedlings can be easily produced by culturing cells near the growth point at the tip of the stem. For example, a plant body (about 5-10 mm) including the stem apex of a seedling in which the 2nd to 5th leaves have expanded is cut out and cultivated by piercing it in 1/2 MS medium like a cutting or placing only the stem. Multiple side buds are observed from the lower part of the plant body (the root and the plant containing cotyledons or first leaves) from which the stem apex is cut. Since armpit bud formation occurs by cutting the stem apex, a growth point can be easily obtained. In the case of only stems without leaves, homogeneous young leaves can be observed from all by simply placing them in the medium.

3 μM, 5 μM, and 10 μM indole butyric acid (IBA) -added medium and non-added medium were prepared in 1/2 MS medium, and cuttings were made. The root was induced. However, since roots were formed earliest in the additive-free medium and root growth was good, it is considered that plant hormones (IBA, etc.) are not particularly necessary.
For subculture, either stem cuttings or stem cuttings (not including the stem apex) may be used, and rooting is performed within approximately 10 days after transplanting to 1/2 MS medium (containing 1% sucrose). do. In the case of only stems without leaves, homogenous young leaves from all and some rooting are observed after 30 days just by placing in the medium.

[Making Itea seedlings]
Plants rooted in a petri dish (cultured for one month or longer until rooting) are transplanted into vermiculite or potting soil, preferably covered with a hood such as a transparent film. It is preferable to use vermiculite rather than potting soil because the growth tends to be suppressed when the potting soil is used for culturing from seedlings. Then, after 2-3 days, the hood is displaced to remove moisture, and after 2-3 days, the hood is completely removed. Regarding this acclimatization step, if a hooded container is used, there is no problem even with seedlings containing only Futaba. It is also possible to raise seedlings by hydroponics using rock wool or the like. During the seedling raising period, it is preferable to cover it with an insect net to prevent damage to Spodoptera litura larvae. The rooted Itea plant is transplanted into the medium or potting soil of the plug tray and grown in the form of plug seedlings.
When the outside temperature becomes low during growth (the minimum temperature is below 15 degrees Celsius), the leaves turn red. Itea does not fall, but Virginia sweetspire falls in winter. Since the growth of saplings is greatly affected by temperature, it is more efficient to start the acclimatization step after early spring and grow them toward summer. The schedule to start working on is good.

[Growth from Itea seedlings]
Similar to the production of Zuina seedlings, the Zuina plant including the stem part was cut from the young Zuina tree, or the Zuina plant including the stem apex or stem part was cut from the seedling in which the 2nd-5th leaves of Zuina were developed. The Zuina plant is left as it is, or the leaves are removed and placed in a rooting medium like a cutting, and this is cultivated for rooting. Etc.) and grow until the young leaf vegetables and / or new leaf vegetables can be harvested, and the young leaf vegetables and / or new leaf vegetables are harvested. As the rooting medium, 1/2 MS medium or Gamborg B5 medium is used. When growing rooted Itea plants into Itea vegetables, the use of a medium enables aseptic growth of functional vegetables based on the functions of D-allulose and alitor.

[Itea freeze-dried]
(When using the entire Itea seedlings aseptically cultured in a petri dish)
The subcultured (48 days) Itea seedlings were manually removed together with the roots without cutting the roots, and the rooting medium (for example, MS medium) was removed as much as possible using a toothpick or the like. Then, the water is wiped off and the raw weight of the Itea seedlings is measured. In this case, about 1 to 4 g of Itea raw weight can be obtained per petri dish. For example, Table 3 is a table showing an example of measurement results of the number of petri dishes and the raw weight of Itea seedlings.

(When using only mature leaves of Itea grown in the field)
Only the mature leaves of Itea grown in the field are cut out with pruning shears and collected in a bag. Then, the raw weight of the mature Itea leaves is measured.
Adult leaves of Itea are soaked in liquid nitrogen and frozen, then placed in a double bag and stored at -80 ° C. The preserved adult leaves of Itea should be crushed as appropriate.

(Common procedure)
Soak Zina in liquid nitrogen to freeze it, put the frozen Zina in a stainless steel beaker chilled in liquid nitrogen, and then cover the beaker with double gauze. Place a 5 liter beaker containing Zina in a vacuum freeze dryer (FREEZONE 12Plus, manufactured by LABCONCO), or place a 2 liter beaker containing Zina in a vacuum freeze dryer (FREEZE DRY SYSTEM / FREEZONE2.5, LABCONCO). It was put into (manufactured by) and freeze-dried for a certain period of time with occasional stirring. Even when Itea was put in a full 5 liter beaker, it dried in about a week. After drying, the weight was measured and found to be about 1/10 of the raw weight. The weighed Itea was transferred to a Mini Speed Mill (Lab Next). After crushing the Itea with a mini speed mill for 10 to 20 seconds, the contents were checked, and this was repeated until the Itea became powdery. If the Itea is continuously crushed, the temperature of the mill becomes high and the color of the Itea powder changes. Therefore, it is preferable to cool the mill or replace the mill. The crushed Itea was collected as much as possible using a spatula or spatula and placed in a container. The lid of the container containing Itea was wrapped with parafilm, shielded from light with aluminum foil, placed in a box, and stored at room temperature in the dark.

[Orally administered body fat accumulation inhibitor or total cholesterol elevation inhibitor, or oral composition]
The orally administered body fat accumulation inhibitor or total cholesterol level elevation inhibitor, or oral composition of the present invention (hereinafter, collectively referred to as "oral composition") is intended for mammals including humans. It is orally administered to the mammal. The oral composition of the present invention is a composition having a function of suppressing body fat accumulation and / or a function of suppressing increase in total cholesterol level, and may be a food, a pharmaceutical product, a food additive, or the like. The oral composition of the present invention utilizes Zuina, a deciduous shrub of the genus Itea of the family Itea, which is a plant body that produces and contains allulose and alitol, which are produced in very small amounts in nature, in the form of a plant body. Although there have been no reports of nutritional studies in the past, it is a plant that used to eat new leaves, so it can be taken on a daily basis without difficulty, so-called functional foods. Suitable for use as food such as.

As described above, the oral composition of the present invention contains Itea in the form of a plant or as a plant extract thereof, but also contains various health ingredients and various additives that are acceptable as foods or pharmaceuticals. It may be included. Other ingredients include various pharmaceutically or food hygiene-acceptable carriers for oral administration, such as excipients, lubricants, stabilizers, dispersants, binders, diluents, flavors, sweeteners, etc. Flavoring agents, coloring agents and the like can be exemplified. The form of the composition of the present invention is not particularly limited as long as it exerts the effect of the present invention, and for example, tablets, pills, granules, fine granules, chewing agents, capsules, liquids, chewable agents, beverages. And so on. It may be in the form of other foods. These dosage forms can be prepared using conventional methods commonly known in the art.

For example, when the oral composition is made into granules, the granulation method is not particularly limited, and any method such as a fluidized bed granulation method or an extrusion granulation method can be used. Above all, a fluidized bed granulation method for obtaining a granulated product having good dispersibility can be preferably used. Usually, when granulating a powdered oral material, a binder such as hydroxypropyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, carboxymethyl cellulose, pullulan, starch, dextrin, guar gum, galactomannan or the like is used.
The obtained granular composition has good dispersibility in water, has a small tendency to scatter as a fine powder, and is easy to package as a product. In general, for health and taste reasons, consumers who have added a large amount of the in vivo functional ingredient Itea to an additive other than a plant, for example, a binder such as the above-mentioned hydroxypropyl cellulose, etc. Not liked by. The binder can be used in an amount within a range that does not impair the solubility and dispersibility of the obtained granular composition when dissolved in water. Although it depends on the strength of the binder used, the binder can be used in an amount of 3% by weight or less when used as a solution and 10% by weight or less when powder is added.

[Rare sugar]
Rare sugars are defined as monosaccharides and their derivatives that are present in nature in trace amounts. For hexacarbonate (hexose), in the case of aldose, L-allose, L-growth, L-glucose, L-galactose, L-altrose, L-idose, L-mannose, L-talose, D-talose, 16 types of D-mannose, D-idose, D-altrose, D-galactose, D-glucose, D-growth, D-allose, L-allose, L-sorbose, L-fluctose, L- There are eight types: tagatos, D-tagatos, D-fructos, D-sorbose, and D-altrose. For example, D-allulose has not been reported to be toxic to humans and is considered to be less toxic to animals.
At present, the method for obtaining D-allulose with a rare sugar is generally a production method obtained by treating D-fructose with an enzyme (epimerase).

As mentioned above, the rare sugar D-allulose has the characteristics of an assimilating sugar, an excellent sweetener, an anti-obesity agent, an eating inhibitor, an insulin sensitizer, and a low-calorie sweetener, and also has low calorie content. Nevertheless, it has new characteristics such as causing suppression of eating. In addition, since its sweetness is close to that of sugar and its calories are low, it is attracting attention as a sugar that can be widely used as a low-calorie sweetener. Rare sugars are monosaccharides excluding D-glucose and D-mannose, which are abundant in nature. For example, D-allose, D-allose, etc. are monosaccharides that are produced in very small amounts in nature. The nature and use are still under development. Currently, rare sugars such as D-allulose can be produced using enzymes and the like, but it has been found that they are also contained in plants such as leaves of Itea and clethra barbiner.
Rare sugars are defined by the International Rare Sugar Society as "monosaccharides and their derivatives whose abundance is small in nature", and when monosaccharides and sugar alcohols as their derivatives are added, there are about 60 types. D-glucose, D-fructose, D-mannose, and D-galactose, which can be produced from lactose in milk, are abundant in nature, and the others are classified as rare sugars, which are present only in trace amounts. The discovery of DTE has made it possible to produce D-fructose and D-allose from D-glucose, as well as D-allose, aritol and D-talitol.

Aritol is a rare sugar of sugar alcohol and is a polyol (sugar alcohol). Specifically, alitol is a sugar alcohol having 6 carbon atoms produced by reducing D-allulose, and is considered to exist in Itea while being redoxed with D-allulose and dynamically moving back and forth. .. The purpose of this embodiment is to elucidate the physiological function of a plant containing both D-allulose and alitol by using Itea, which is a plant containing D-allulose and alitol, and to elucidate the physiological function of the plant containing both D-allulose and alitol. There are many unclear points about the physiological function, and it is thought that it will be clarified in future research.

[Dietary fiber-containing material]
The oral composition of the present invention can further contain a dietary fiber-containing material. Preferred examples of dietary fiber-containing materials are tea powders selected from Japanese tea, black tea and Chinese tea.
Since the oral composition of the present invention may be in the form of tablets, capsules, granules, powders, troches and the like, for example, a dietary fiber-containing material similar to the crushed or crushed lyophilized product of Zuina is added. Even in this case, the granules can be satisfactorily granulated. Many studies have been conducted on the main actions of dietary fiber in the fields related to protein metabolism, lipid metabolism, and sugar metabolism. In addition, research on the physiological effects on bacterial groups existing in the intestine is also attracting attention. Dietary fiber is abundantly contained in parts such as leaves, buds, stems, flowers, fruits, roots, ears, seeds, and fruits, which mainly contain plant fiber. Dietary fiber-containing materials are typically materials containing the fibers of green plants. A preferred example is a food material for producing so-called green juice. For example, Angelica keiskei, young wheat leaves, green tea, kale, young kansho leaves, broccoli, moroheiya, young sesame leaves, mulberry leaves, kale siler, yomogi and the like, and mixtures thereof. The shape and manufacturing method of the dietary fiber-containing material are not particularly limited, but it is preferably in the form of powder, and is preferably a powder of 75 μm or less.

[Body fat accumulation suppression function and total cholesterol level increase suppression function]
The inventor has found that, as shown in Examples 1 and 2 described later, the Itea dry powder has a function of suppressing body fat accumulation and a function of suppressing increase in total cholesterol level as physiological actions. Such a physiological action was observed in a rare sugar extracted from a natural product, but in the present invention, even a dry powder of Zuina, which is not an extracted rare sugar, has a function of suppressing body fat accumulation and a function of suppressing an increase in total cholesterol level. It turned out. This suggests that the Itea dry powder itself can be used as a raw material for an agent having a function of suppressing body fat accumulation and a function of suppressing increase in total cholesterol level.

In addition to the above, the oral composition of the present invention may contain additives or auxiliary ingredients commonly used in foods or pharmaceuticals. For example, high sweetness sweeteners such as starch, lactose, crystalline cellulose, martitol, dextrin, purulan, guagam as excipients, aspartame, sclarose, acesulfam K, stevia, monatin, monerin, miraculin as sweeteners, sorbitol, xylitol. , Mannitol, erythritol, lactitol, martitol and other sugar alcohols, and sweeteners such as ketose and aldose may be further mixed to adjust the sweetness, and carotene, L-aspartame, α-tocopherol and lutein may be used as fortifiers. , Ricopin and the like may be included.

The dosage form of a drug (including non-pharmaceutical products) may be, for example, an injection, a suppository, an inhalant, a transdermal absorbent, various external preparations, tablets, capsules, granules, powders, syrups, etc. The administration form may be either oral administration (internal use) or parenteral administration (external use, injection). In order to prepare pharmaceutical preparations of such various dosage forms, for example, the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in the natural world of the present invention, are produced and contained in the plant body. Plants or other pharmaceutically acceptable excipients, binders, bulking agents, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, flavoring agents, fragrances, coatings Agents, carriers, diluents, other medicinal ingredients and the like can be used in appropriate combinations.

Examples of the medicinal ingredient that can be blended in the above-mentioned pharmaceutical products (including quasi-drugs) include vitamins, drugs known to promote fat metabolism, natural products, and the like. That is, in addition to the above, the oral composition of the present invention includes vitamins, vitamin-like substances, proteins, amino acids, fats and oils, organic acids, carbohydrates, plant-derived raw materials, animal-derived raw materials, microorganisms, food additives, and pharmaceuticals. Ingredients that can be taken orally, such as additives, can be appropriately contained.

When a plant containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, is used as food, it can be carried out by a usual method. .. Specifically, for example, it can be carried out as described below. As a food, a plant containing a rare sugar D-allulose and a sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, or a plant extract thereof, and is orally produced by an animal. There are no particular restrictions on the type, shape, etc. of the food as long as it can be ingested. Foods include, for example, sweets such as drops, candy, ramune, gummy, and chewing gum; Western sweets such as cookies, crackers, biscuits, potato chips, cakes, chocolates, donuts, puddings, and jellies; Japanese sweets such as buns and castella; cold sweets such as ice cream, ice candy, sherbet, gelato; breads such as bread, French bread, croissants; noodles such as udon, buckwheat, Chinese noodles, and sardines; kamaboko, chikuwa, fish sausage, etc. Fish paste products; Livestock products such as ham, sausage, hamburger, corn beef; salt, pepper, miso, soy sauce, sauce, dressing, mayonnaise, ketchup, sweeteners (eg sugar, honey, powdered candy, water candy, jam, marmalade, etc. ), Seasonings such as spicy seasonings (for example, salad, pepper, etc.); Various side dishes such as natto, thick fried, tofu, konjac, dumplings, pickles, boiled noodles, dumplings, shumai, croquette, sandwiches, pizza, hamburgers, salads; livestock products such as beef, pork, chicken; shrimp, scallops, 蜆, Marine products such as kelp; various powders of vegetables / fruits, plants, yeasts, algae, etc .; oils / fats / fragrances (vanilla, citrus, bonito, etc.) powdered and solidified; beverages, etc. may be mentioned.

Beverages include foods and drinks such as soup and miso soup; powdered foods and drinks such as instant coffee, instant tea, instant milk, instant soup and instant miso soup; whiskey, bourbon, spirits, liqueurs, wine, fruit wine, sake, Chinese sake, Shochu, beer, non-alcoholic beer with an alcohol content of 1% or less, sparkling liquor, alcoholic beverages such as liquor high; beverages containing fruit juice (for example, apple, orange, grape, banana, pear, ume fruit juice, etc.), vegetable juice (for example) , Tomato, carrot, celery, cucumber, watermelon vegetable juice, etc.), fruit juice and vegetable juice, soft drink, milk, soy milk, dairy drink, drink type yogurt, coffee, cocoa, tea drink (tea, Green tea, barley tea, brown rice tea, roasted tea, tamaro tea, roasted tea, oolong tea, ukon tea, puer tea, louis bostee tea, rose tea, kiku tea, herb tea (for example, mint tea, jasmine tea), nutritional drinks, sports Beverages, non-alcoholic beverages such as mineral water and the like can be mentioned.

Preferred examples of such food compositions include jelly, tea beverages, alcoholic beverages, drops, candies, ramunes, cookies, crackers, biscuits, chocolate, cheese, butter, margarine, chewing gum and the like.

Further, the food of the present invention is prepared as a functional food, a health food, a food for specified health use, a food with a health function such as a food with a nutritional function, a food for special use (for example, a food for the sick), a health supplement, a supplement and the like. It may be prepared as a food for specified health use, a food for special use, a dietary supplement or a supplement.

The shapes of such foods include, for example, tablets, rounds, capsules (including hard capsules, soft capsules, and microcapsules), powders, granules, fine granules, troches, and liquids (syrups, emulsions, suspensions). (Including turbidity) and the like, preferably in the form of tablets or capsules.

The food of the present invention is particularly preferably a tablet-shaped or capsule-shaped food for specified health use, a special-purpose food, a dietary supplement, or a supplement. Here, the supplement not only means a dietary supplement for supplementing nutrients, etc., but also has functions useful for maintaining, recovering, and promoting health (for example, anti-weight gain suppression, body fat accumulation suppression, etc.). It also means a dietary supplement having an obesity effect or a slimming effect).

The food of the present invention is, for example, a plant or a plant containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in the food by a known method in a very small amount in nature. It can be produced by adding a body extract. Specifically, for example, tablet-shaped foods produce and contain the rare sugar D-allulose and the sugar alcohol rare sugar antitol, which are produced and contained in the plant body in a very small amount in nature. It can be produced by adding and mixing raw materials such as an agent (for example, lactose, sucrose, mannitol, etc.), a sweetener, a flavoring agent, etc., and applying pressure with a tableting machine or the like to form a tablet. If necessary, other materials (for example, vitamins such as vitamin C, minerals such as iron, dietary fiber, etc.) can be added. The capsule-shaped food composition contains, for example, a plant body or a plant body extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced and contained in the plant body in a very small amount in nature. It can be produced by filling a capsule with a liquid, suspended, paste-like, powder-like, or granular food composition, or by encapsulating it with a capsule base.

The food of the present invention is physiologically added to commonly used food materials, food additives, various nutrients, vitamins, flavor substances (for example, cheese, chocolate, etc.), etc., as long as the effects of the present invention are not impaired. An acceptable carrier or the like can be blended. As physiologically acceptable carriers and the like, various conventional organic or inorganic carrier substances are used, and excipients, binders, disintegrants, thickeners, colorants, sweeteners, preservatives, antioxidants, etc. Thickeners, emulsifiers and the like can be mentioned. Examples of food additives include colorants, sweeteners, preservatives, antioxidants, flavoring agents and the like. Further, it may contain other materials such as minerals such as iron and dietary fiber such as pectin, carrageenan and mannan.

The excipients, binders, disintegrants, lubricants, solvents, solubilizers, suspending agents, buffers, thickeners, colorants, sweeteners, preservatives, and antioxidants are described above. Examples thereof include those used in the preparation of the present invention.

Vitamins may be water-soluble or fat-soluble, and may be, for example, retinol palmitate, tocopherol, bisbenchamine, riboflavin, pyridoxin hydrochloride, cyanocobalamin, sodium ascorbate, choleciferol, nicotinamide, pantoten. Examples thereof include calcium acid, folic acid, biotin, choline bitartrate and the like.

For tablet-like, granular, fine-grained food compositions, etc., they are coated by a method known per se using a coating base material for the purpose of masking the taste, improving photostability, improving the appearance, or entericing. You may. Examples of the coating base material include those similar to those used in the above-mentioned pharmaceutical product of the present invention, which can be carried out in the same manner.

Health products such as health supplements, foods for specified health use, etc. Plants that produce and contain the rare sugar D-allulose and the sugar alcohol rare sugar aritol, which are produced in very small amounts in nature when added to special-purpose foods such as functional foods and foods for the sick. It is preferable that the intake of the body or the plant extract at one time is contained in the beverage or food in the form of being packaged or filled in units of one meal intake. Here, the "beverage or food in the form of being packaged or filled in units of one meal intake" means that the amount of beverage or food to be ingested at one time is packaged in a container such as one bag, box, or bottle. Or it means that it is filled.

Further, the food of the present invention may be used by itself, or may be used in combination with other pharmaceutical compositions, food compositions, or feeds having an anti-obesity effect or a blood triglyceride lowering effect. When used in combination, the anti-obesity effect such as the effect of suppressing weight gain and the effect of suppressing body fat accumulation, the slimming effect or the prevention and improvement effect of hypertriglyceridemia can be further enhanced.

The oral composition of the present invention may be provided with a functional label. There are no particular restrictions on the method of labeling the function, but labeling on food packaging, container surfaces, food manuals, food advertisements, etc. can be exemplified. Examples of the functions of the oral composition of the present invention include a function of suppressing body fat accumulation and / or a function of suppressing increase in total cholesterol level. The above foods have the concept of suppressing body fat accumulation and / or suppressing the increase in total cholesterol level, and indicate that fact as necessary, such as foods for the sick, nutritionally functional foods, health foods, or specified health foods. Functional foods such as foods for use are included. Here, the functional label attached to these foods can be any of the main body of the product, the container, the packaging, the instruction manual, the package insert, or the advertisement. The form of the food can be solid, semi-solid or liquid.

Use as foods, pharmaceuticals, food additives, etc. may be in humans or non-human animals, or in specimens derived from them, and may be therapeutic or non-therapeutic. Here, "non-therapeutic" means a concept that does not include medical practice, that is, a concept that does not include a method of surgery, treatment, or diagnosis of a human being, and more specifically, a doctor or a person who has been instructed by a doctor. On the other hand, it is a concept that does not include a method of performing surgery, treatment, or diagnosis.
The intake of the food of the present invention is the body fat of a plant or a plant extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced and contained in the plant body in a very small amount in nature. It may be within the range of an effective amount that suppresses accumulation or within the range of an effective amount that lowers the total cholesterol level. The content of the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in the food of the present invention in a very small amount in nature, is usually about about the total content of the food. 0.1 to 20% by mass, preferably about 0.5 to 10% by mass, more preferably about 1 to 5% by mass. As a guide, the content of the plant extract is about 10% of the plant itself. The effect is not exhibited even if the content of the plant body or the plant body extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, in the plant body is too small. ..
For example, when the food of the present invention is ingested by an adult for the purpose of suppressing body weight gain, the rare sugar D-allulose and sugar alcohol rare, which are produced in a very small amount in nature, vary depending on the subject to be ingested, the form of intake, the amount of food intake, and the like. The intake of a plant containing the sugar allytol produced in the plant is generally 1 to 400 mg, preferably 40 to 400 mg, per 1 kg of body weight per day and as a dry weight. The above amount may be ingested once, or may be ingested in several divided doses. The effect is not exhibited even if the dose of the plant body or the plant body extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, is produced in the plant body. .. In addition, each of the above intakes is preferable from the viewpoint that the effect is exhibited without affecting the palatability and the amount of food intake. Similar amounts can be ingested for other animals.
The food thus obtained is safe and can be continuously fed to vertebrates, preferably mammals, particularly preferably humans.

A plant or a plant extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced and contained in the plant body, which are produced in a very small amount in nature in the above-mentioned pharmaceutical products (including non-medicinal products). The content is usually 0.1% by mass or more, preferably 1.0% by mass or more, and 95% by mass or less, preferably 80% by mass or less, more preferably 60% by mass or less, based on the total mass of the preparation. ..
The content of the plant body or the plant body extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in the above-mentioned foods in a very small amount in nature, is the use thereof. Although it depends on the form, it is usually 0.01% by mass or more, and 50% by mass or less, preferably 20% by mass or less, and more preferably 10% by mass or less.
A plant or a plant extract containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in the natural world of the present invention, as a drug or supplement, or a drug or When used in combination with supplements, the dose depends on the morphology of the plant, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature. Rare sugar D, which can vary depending on the subject's condition, weight, gender, age or other factors, but when given orally, the daily dose per adult usually produces very small amounts in nature. -As a plant or a plant extract containing allulose and the sugar alcohol rare sugar alitol produced and contained in the plant body, it is 15 mg or more, and 10 g or less, preferably 5 g or less, still more preferably 1 g or less.
Further, although the above-mentioned preparation can be administered according to an arbitrary administration plan, it is preferable to administer it once to several times a day and continuously for several weeks to several months.
The target of administration or intake is not particularly limited as long as it is an animal that needs or desires it, but a person who needs or desires promotion of energy consumption, suppression of body fat accumulation, or prevention or improvement of obesity. Can be mentioned.

The first and second embodiments will be described below.

First, Itea dry powder was produced from Itea (seedlings). The results of nutritional component analysis of Itea dry powder are shown in Table 4. As shown in Table 4, the Itea dry powder used in Example 1 contains about 10 g of alitor and 8 to 9 g of D-allulose (denoted as D-psicose in the chart) per 100 g of the Itea dry powder. The content of rare sugar corresponded to about 5% of the dry powder of Itea.

In Example 1, 16 male Wistar rats aged 3 weeks were preliminarily bred, and then divided into 8 control diet groups and 8 Itea diet groups, respectively, and bred for 8 weeks. The dietary composition of each group is shown in Table 5.

Each diet is a high-fat and high-sucrose diet and contains the same amount of protein, sugar and lipid. Further, the Itea diet contains 5% of the total weight of the food, that is, 50 g of the dry powder of Itea with respect to 1000 g of the total weight of the food.

Then, the rats of each diet group were bred for 8 weeks under the following breeding conditions. Specifically, the light-dark cycle was defined as a light period from 8:00 to 20:00 and a dark period from 20:00 to 8:00. The temperature was 22 ± 1 ° C., and food and water were freely ingested. Body weight and food intake were then recorded daily. Then, after the end of the breeding period, rats in each diet group were sacrificed and dissected without fasting.

Rats in each diet group were bred for 8 weeks, then slaughtered and dissected, and serum components and tissue components were collected as shown in FIG. Then, using each of the collected tissues, the inspection shown in FIG. 1 was performed. Note that FIG. 1 is a diagram for explaining the collected tissue and the inspection items in the first embodiment.

FIG. 2 is a graph showing the transition of the average body weight of rats in each diet group after 8 weeks of breeding in Example 1. As shown in FIG. 2, the rats in the Itea diet group tended to have lower body weight than the rats in the control diet group, but the difference was not significant.

In addition, FIG. 3 is a graph showing the final body weight, body weight gain, food intake and food efficiency of rats in each diet group. The final body weight, weight gain, dietary intake and dietary efficiency also tended to be lower in the rats in the Zina diet group than in the rats in the control diet group, but there was no significant difference.

FIG. 4 is a graph showing the relative tissue weights of the heart, liver, spleen and kidneys of rats in each diet group when the weight of rats is 100 g. As shown in FIG. 4, there was no significant difference between the rats in the Zina diet group and the rats in the control diet group for the heart, spleen and kidneys, but for the liver, the rats in the Zina diet group were in the control diet group. The weight was significantly lower than that of the rats.

FIG. 5 is a graph showing the relative tissue weights of perirenal adipose tissue, accessory testicular adipose tissue, mesenteric adipose tissue, and total abdominal adipose tissue of rats in each diet group when the weight of the rat is 100 g. .. As shown in FIG. 5, regarding perrenal adipose tissue, accessory testicular adipose tissue, mesenteric adipose tissue, and total intraperitoneal adipose tissue, rats in the Zina diet group have lower weight than rats in the control diet group. There was a tendency, but the difference was not significant.

FIG. 6 is a graph showing total protein, lipase, total cholesterol, and crawl among the serum components of rats in each diet group. As shown in FIG. 6, rats in the Zina diet group showed significantly lower values for total protein, lipase, and total cholesterol than rats in the control diet group. On the other hand, regarding crawl, the rats in the Itea diet group showed significantly higher values than the rats in the control diet group. No significant difference was observed except for the serum components listed in FIG.

FIG. 7 is a graph showing glycogen, total fat, cholesterol and triglyceride among the liver components of rats in each diet group. As shown in FIG. 7, rats in the Zina diet group tended to have lower amounts of liver glycogen, total fat, cholesterol and triglyceride than rats in the control diet group, but to a significant difference. There wasn't. It is considered that one of the factors that reduced the liver weight in the rats in the Itea diet group as compared with the rats in the control diet group was the decrease in fat accumulation.

FIG. 8 is a graph showing the carcass fat mass, carcass fat percentage, total body fat mass, and total body fat percentage of rats in each diet group. As shown in FIG. 8, rats in the Zuina diet group showed significantly lower values for carcass fat mass, carcass fat percentage, total body fat percentage, and total body fat percentage than rats in the control diet group. ..

(About the toxicity of Itea dry powder)
As shown in FIG. 6, the total protein value of the rats in the Itea diet group was significantly lower than that of the rats in the control diet group. Total protein is a guide for knowing the condition of the liver and kidneys, but among the serum components not shown, ALS and AST, which are indicators of liver function, urea nitrogen and creatinine, which are indicators of renal function, and serum protein abnormalities. In the A / G ratio, which is an index of the above, there is no significant difference between the rats in the Zina diet group and the rats in the control diet group, and it is considered that the changes are usually within the range of possible changes.
In addition, as shown in FIG. 6, the total cholesterol value of the rats in the Itea diet group was significantly lower than that of the rats in the control diet group. Total cholesterol is an indicator of dyslipidemia, but there was no significant difference in HDL cholesterol, non-HDL cholesterol and triglyceride (not shown). As shown in FIG. 7, the cholesterol level in the liver also tends to be lower in the rats in the Zina diet group than in the rats in the control diet group. Significantly lower levels compared to rats in the control diet group are suggested to be due to the components in Zina.
As shown in FIG. 6, the rats in the Zina diet group showed significantly lower values for lipase than the rats in the control diet group, and the rats in the Zina diet group showed significantly lower values for chlor in the control diet group. The value was significantly higher than that of the rat. Lipase is an index of pancreatic disease, and chlor is involved in the regulation of pH and osmotic pressure. Since both of them are components affected by diet, blood should be collected on an empty stomach. It is probable that there was a significant difference because the animals were slaughtered and dissected without fasting.
As shown in FIG. 6, among the serum components, total protein, lipase, total cholesterol and chlor were significantly different between the rats in the Zina diet group and the rats in the control diet group, but the Zina dry powder was toxic. It turned out not.

(Itea dry powder body fat accumulation suppression function)
As shown in FIG. 8, rats in the Zuna diet group showed significantly lower values for carcass fat and total body fat than rats in the control diet group. This suggests that Itea dry powder has a function of suppressing body fat accumulation.

(Function of suppressing increase in total cholesterol level of Itea dry powder)
As shown in FIG. 6, the total cholesterol level of the serum components was significantly lower in the rats in the Zina diet group than in the rats in the control diet group. In addition, as shown in FIG. 7, the cholesterol in the liver tissue tended to be lower in the rats in the Itea diet group than in the rats in the control diet group. This suggests that Itea dry powder has a function of suppressing an increase in total cholesterol level.

As described above, it was found that the dry powder of Itea is not toxic and has a function of suppressing body fat accumulation and a function of suppressing increase in total cholesterol level. Such a function is considered to be due to the action of the rare sugars D-allulose and alitol contained in Itea. Therefore, the following Example 2 was carried out in order to investigate whether there is a difference in function between the dry powder of Itea and D-allulose and alitol extracted from natural products.

First, Itea dry powder was produced from Itea (seedlings). The results of nutritional component analysis of Itea dry powder are shown in Table 6. As shown in Table 6, the Itea dry powder used in Example 2 contains 12.1 g of alitol and 7.8 g of D-allulose (denoted as D-psicose in the chart) per 100 g of the Itea dry powder. , Corresponds to about 5% of Itea dry powder.

In Example 2, 32 male Wistar rats aged 3 weeks were pre-reared and then divided into 8 control diet group, 8 Zina diet group, 8 rare sugar diet group, and 8 green tea diet group, respectively. It was divided and bred for 8 weeks. The dietary composition of each group is shown in Table 7.

Each diet is a high-fat and high-sucrose diet and contains the same amount of protein, sugar and lipid. Further, in the Itea diet, the Itea dry powder is contained in 5% of the total weight of the diet, that is, 50 g of the Itea dry powder is contained with respect to 1000 g of the total weight of the diet. Similarly, in a green tea diet, dry green tea powder is contained in 5% of the total weight of the diet, that is, 50 g of dry green tea powder is contained in 1000 g of the total weight of the diet. In addition, 50 g of Itea powder contained in the Itea diet contains 3.9 g of D-allulose (denoted as D-psicose in the chart) and 6.1 g of aritol, so that the rare sugar diet is extracted from natural products. The same amount of D-allulose and allitol was mixed with the Itea diet, that is, 3.9 g of D-allulose and 6.1 g of allitol were mixed.

Then, the rats of each diet group were bred for 8 weeks under the following breeding conditions. Specifically, the light-dark cycle was defined as a light period from 8:00 to 20:00 and a dark period from 20:00 to 8:00. The temperature was around 25 ° C, the humidity was around 68%, and food and water were freely ingested. Body weight and food intake were then recorded daily. Then, after the end of the breeding period, rats in each diet group were sacrificed and dissected without fasting.

Rats in each diet group were bred for 8 weeks, then slaughtered and dissected, and each tissue component of the rats was collected as shown in FIG. Then, the inspection items shown in FIG. 9 were inspected using each of the collected tissues. Note that FIG. 9 is a diagram for explaining the collected tissue and the inspection items in Example 2.

FIG. 10 is a graph showing body weight transition, final body weight, food intake and food efficiency of rats in each food group. In the following, the rats in the control diet group will be referred to as the C group, the rats in the Zina diet group will be referred to as the IT group, the rats in the rare sugar diet group will be referred to as the RS group, and the rats in the green tea diet group will be referred to as the G group. Regarding body weight transition and final body weight, the weight of rats in the green tea diet group (G group) tends to be low, and is significant only between the rats in the Zuna diet group (IT group) and the rats in the green tea diet group (G group). A difference was observed. There was no significant difference in food intake between the rats in each diet group, but the diet efficiency was significantly lower in the green tea diet group rats (G group) than in the other diet group rats.

FIG. 11 is a graph showing the relative tissue weights of the heart, liver, kidney and spleen of rats in each diet group when the weight of rats is 100 g. As shown in FIG. 11, there was no significant difference between the rats in each diet group for the heart and kidneys, but for the liver and spleen, the rats in the green tea diet group (Group G) were the rats in the other diet groups. It was a low value compared to. Specifically, for the liver, the rats in the green tea diet group (G group) were significantly lower than those in the Zina diet group (IT group), and for the spleen, the Zina diet group and the rare sugar group. The rats (G group) in the green tea diet group were significantly lower than those in the rat (IT group, RS group).

FIG. 12 is a graph showing the relative tissue weights of the accessory testicle adipose tissue, the perirenal adipose tissue, the mesenteric adipose tissue, and the total intraperitoneal adipose tissue of the rats in each diet group when the weight of the rat is 100 g. .. As shown in FIG. 12, in all of the accessory testicular adipose tissue, perineal adipose tissue, mesenteric adipose tissue, and total intraperitoneal adipose tissue, rats (G group) in the green tea diet group were selected from the control diet group and the Zina diet group. The values were significantly lower than those of rats in the rare sugar diet group (C group, IT group, RS group). In addition, the perirenal adipose tissue and the total intra-abdominal adipose tissue showed significantly lower values in the rats in the Zina diet group (IT group) than in the rats in the control diet group (C group).

FIG. 13 is a graph showing the carcass fat mass, carcass fat percentage, total body fat mass, and total body fat percentage of rats in each diet group. As shown in FIG. 13, regarding the total fat mass, the rats in the green tea diet group (G group) were compared with the rats in the control diet group, the Zuna diet group and the rare sugar diet group (C group, IT group, RS group). The value was significantly lower. In addition, the carcass fat percentage also showed significantly lower values in the green tea diet group rats (G group) than in the control diet group and the rare sugar diet group rats (C group, IT group, RS group). rice field. Furthermore, the total fat ratio was significantly lower in the rats in the Zina diet group (IT group) than in the rats in the control diet group (C group), and further in the rats in the green tea diet group (G group). It showed a low value.

FIG. 14 is a graph showing the test results of glucose, insulin and triglyceride concentrations in the serum components of rats in each diet group. As shown in FIG. 14, there was no significant difference in glucose concentration among serum components among rats in each diet group, but insulin concentration and triglyceride concentration were observed in rats in the green tea diet group (Group G). ) Showed significantly lower values than the rats (C group, IT group, RS group) in the control diet group, the Zina diet group and the rare sugar diet group.

FIG. 15 is a graph showing the test results of total cholesterol, HDL cholesterol, and free fatty acid concentrations in the serum components of rats in each diet group. As shown in FIG. 15, among the serum components, the total cholesterol concentration and the free fatty acid concentration are significantly lower in the green tea diet group rats (G group) than in the control diet group rats (C group). The value is shown. Regarding the concentration of HDL cholesterol, the rats in the Zina diet group and the rare sugar diet group (IT group, RS group) showed significantly lower values than the rats in the control diet group (C group), and the green tea diet group. The value was even lower in the rats (Group G).

(Anti-obesity function of Itea dry powder)
As shown in FIGS. 12 and 13, the rats in the Zuina diet group (IT group) were compared with the rats in the control diet group (Group C) in terms of perirenal adipose tissue, total intra-abdominal adipose tissue, and total body fat percentage. The value was significantly lower. From this, it was suggested that the dry powder of Itea also has a function of suppressing body fat accumulation in Example 2. In addition, as shown in FIG. 15, HDL cholesterol and free fatty acids among the serum components were also significantly lower in the rats in the Zina diet group (IT group) than in the rats in the control diet group (C group). became.
In addition, in the rare sugar diet group rats (RS group), adipose tissue of accessory testicle adipose tissue, perineal adipose tissue, and adipose tissue of mesenteric adipose tissue and total intraperitoneal adipose tissue, insulin, neutral fat, free fatty acid, total Serum components of cholesterol and HDL cholesterol, as well as carcass fat mass, carcass fat percentage, total fat mass and total fat percentage The body fat of the body showed the same tendency as that of the Zina diet group rats (IT group), and the control diet. It showed a relatively low value as compared with the rat (group C) of the group.
Therefore, it is suggested that the Itea dry powder has an anti-obesity function under the conditions of a high-fat and high-sucrose diet, similar to the rare sugar extracted from natural products.

In particular, since the test results tend to be the same in the rats in the Zina diet group (IT group) and the rats in the rare sugar diet group (RS group), such a function is a function of the rare sugar D-allulose in the Zina powder. It is considered to be the action of Aritol). In Example 2, although the dietary addition amount of D-allulose was only 0.4%, the anti-obesity function was suggested. It is considered that this is due to the effect of (containing 0.6%) or the synergistic effect of D-allulose and alitol.

(Combined with green tea food)
Further, in Example 2, the rats (Group G) in the green tea diet group showed very low values in body weight, dietary efficiency, total intraperitoneal fat, serum component, and body fat as compared with other dietary groups, and the green tea diet group showed very low values. It is considered that the anti-obesity effect that it has has acted. Therefore, it is expected that the anti-obesity effect will be exhibited more efficiently by taking the dried Itea powder mixed with a dietary fiber-containing material such as green tea.

According to the present invention, the plant itself containing the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in a very small amount in nature, can be easily ingested as an oral preparation, and body fat. The industrial usefulness of the present invention is high because it can be expected to have an effect of suppressing accumulation, an effect of suppressing an increase in total cholesterol value, and the like.

Claims (33)

The untreated plant itself, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature, physically, chemically or biologically. A body fat accumulation inhibitor, characterized in that the treated product itself obtained by treatment or the extract itself of a plant body containing D-allulose and alitol is used as an active ingredient, and the plant body is Zina or Ryobu. Or, a total cholesterol level increase inhibitor. The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to claim 1, wherein the plant is a plant that has been treated to increase the rare sugar content. The plant can be grown by adult trees, mature trees treated with D-allulose augmentation, and / or buds, leaves, branches, flowers or roots of any of these variants, or germinated seeds, subcultures or tissue cultures. The body fat accumulation inhibitor according to claim 1 or 2 , or the total cholesterol level increase inhibitor, which is an entire grown plant. The body fat accumulation inhibitor according to any one of claims 1 to 3 , or the total cholesterol level increase inhibitor, wherein the plant is a dried product. The body fat accumulation inhibitor according to claim 4 , or the total cholesterol level increase inhibitor, wherein the dried product is a freeze-dried product. The freeze-dried product according to claim 5 , wherein the freeze-dried product is a freeze-dried product obtained by a freeze-drying device for a liquid nitrogen-immersed frozen product, or an agent for suppressing an increase in total cholesterol level. The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to claim 5 or 6 , wherein the freeze-dried product is a crushed or crushed product of the freeze-dried product. The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to claim 7 , wherein the crushed or crushed product is a powder. Extracts of the plant is, body fat accumulation inhibitor according to any one of claims 1 is water extract or alcohol extract of leaves aqueous medium extract of plant body 3, or elevated total cholesterol Inhibitor. The body fat accumulation inhibitor or total cholesterol level increase inhibitor according to any one of claims 1 to 9 , wherein the plant or an extract of the plant is in the form of powder, paste or solution. The body fat accumulation inhibitor or the total cholesterol level elevation inhibitor according to any one of claims 1 to 10, which is orally administered. The untreated plant itself, which produces and contains the rare sugar D-allulose and the sugar alcohol rare sugar alitol, which are produced in very small amounts in nature, physically, chemically or biologically. It is characterized in that the treated body itself obtained by the treatment and / or the extract itself of the plant body containing D-allulose and alitol is used as an active ingredient, and the plant body is Zina or Ryobu, which suppresses the accumulation of body fat. An oral composition having a function and / or a function of suppressing an increase in total cholesterol level. The oral composition according to claim 12 , wherein the plant is a plant that has been treated to increase the rare sugar content. The plant can be grown by adult trees, mature trees treated with D-allulose augmentation, and / or buds, leaves, branches, flowers or roots of any of these variants, or germinated seeds, subcultures or tissue cultures. The oral composition according to claim 12 or 13 , which is the whole grown plant. The oral composition according to any one of claims 12 to 14 , wherein the plant is a dried product. The oral composition according to claim 15 , wherein the dried product is a freeze-dried product. The oral composition according to claim 16 , wherein the freeze-dried product is a freeze-dried product obtained by a freeze-drying apparatus for a liquid nitrogen-immersed frozen body. The oral composition according to claim 16 or 17 , wherein the lyophilized product is a crushed or crushed product of the lyophilized product. The oral composition according to claim 18 , wherein the crushed or crushed product is a powder. The oral composition according to any one of claims 12 to 14 , wherein the extract of the plant is a water extract or an alcohol extract of the residue of the aqueous medium extract of the plant. The oral composition according to any one of claims 12 to 14 , wherein the plant or an extract of the plant is in the form of powder, paste or solution. The oral composition according to any one of claims 12 to 21 , further comprising a dietary fiber-containing material. The oral composition according to claim 22 , wherein the dietary fiber-containing material is a tea powder selected from Japanese tea, black tea and Chinese tea. The oral composition according to claim 22 , wherein the dietary fiber-containing material is a powder of a green plant. The oral composition according to claim 22 , wherein the dietary fiber-containing material comprises one or more of Angelica keiskei, young wheat leaves, green tea, kale, young kansho leaves, broccoli, moroheiya, young sesame leaves, mulberry leaves, button siler, and yomogi. The oral composition according to any one of claims 12 to 25 , which is a food product, a quasi drug, or a pharmaceutical product. The oral composition according to claim 26 , which is used as a health food, a health drink or a pharmaceutical product for suppressing body fat accumulation. The oral composition according to claim 26 , which is used as a health food, a health drink or a pharmaceutical product for suppressing an increase in total cholesterol level. 26 to claim 26, wherein the food is prepared as a supplement, a functional food, a health food, an enteric nutrition food, a special purpose food, a health supplement, a food for specified health use, or a food with a nutritional function. 28. The oral composition according to any one of 28. The oral composition according to claim 29 , wherein the special purpose food is a food for the sick. The oral composition according to any one of claims 12 to 30 , which is prepared in the form of tablets, rounds, capsules, powders, granules, fine granules, troches, or liquids. .. The oral composition according to claim 31 , which is a granule or a tablet. The foods are prepared as beverages, confectionery, cold confectionery, noodles, processed marine foods, processed livestock foods, dairy products, fats and oils and processed fats and oils, seasonings, enteric nutritional foods, soups, stews, prepared foods, pickles, or bread. The oral composition according to any one of claims 26 to 32.

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