JP6974245B2 - Capillary and pipette using it - Google Patents

Description

The present disclosure relates to capillaries and pipettes using them.

Conventionally, a pipette is known in which a plurality of types of liquids are sucked into a probe and then reciprocated in the length direction of the probe to be stirred and mixed (see, for example, Patent Document 1 and Patent Document 2). ..

Japanese Unexamined Patent Publication No. 10-62437 Japanese Unexamined Patent Publication No. 2000-304754

The capillary of the present disclosure includes a first end surface located at the end in the first direction, a first opening opened in the first end surface, and a second end surface located at the end in the second direction opposite to the first direction. It has a tubular shape having a second opening that opens to the second end surface and the second opening. The first end face is inclined with respect to the first direction. The first end face has a first end located at the end in the first direction. The distance between the first end and the portion of the first opening closest to the first end is the largest in the portion other than the first end in the first end surface and the portion other than the first end in the first opening . Greater than the distance to the near part. Then, it is relatively located on the first direction side, and is located relatively on the second direction side with the first portion for contacting the liquid, which has the first end surface and the first opening. It has a second portion having the second end face and the second opening for analyzing an internal liquid using a fluorescence reaction.

The pipette of the present disclosure has a capillary, a pressure chamber, and a drive unit. The pressure chamber is connected to the inside of the capillary through the second opening. Then, the drive unit changes the volume of the pressure chamber.

It is a perspective view which shows the specific example of the capillary of this disclosure schematically. It is sectional drawing which shows typically the specific example of the capillary of this disclosure. It is a top view which shows typically the shape of the 1st end face in the specific example of the capillary of this disclosure. It is sectional drawing which shows typically the specific example of the pipette of this disclosure. It is a graph which shows an example of the change of voltage in the signal which drives a drive part schematically.

In the conventional pipette described above, it is necessary to mix a plurality of kinds of liquids in the correct ratio, and therefore it is necessary to collect an accurate amount of each liquid. However, it has been newly clarified by the studies of the inventors that there is a problem that a slightly larger amount of liquid is collected than the intended amount when collecting the liquid. Then, after the capillary was pulled out from the collected liquid, a phenomenon occurred in which the liquid adhering to the tip of the capillary entered the inside of the capillary, and this phenomenon was found to be the cause of the above-mentioned problem. The phenomenon that the liquid adhering to the tip of the capillary enters the inside of the capillary is considered to be caused by the surface tension of the liquid.

The capillaries and pipettes of the present disclosure can remedy such problems. Hereinafter, specific examples of the capillaries and pipettes of the present disclosure will be described with reference to the drawings.

FIG. 1 is a perspective view schematically showing a capillary 10 which is a specific example of the capillary of the present disclosure, FIG. 2 is a cross-sectional view of the capillary 10, and FIG. 3 is a shape of a first end surface 11a of the capillary 10. Is a plan view schematically showing.

The capillary 10 has a cylindrical shape and has a first end surface 11a, a second end surface 12a, a first opening 11b, and a second opening 12b. The "cylindrical shape" means a shape that is long and hollow in one direction and has both ends open, and does not mean only a cylindrical shape. The first end surface 11a is located at the end in the first direction (+ x direction in the figure), and the first opening 11b is opened in the first end surface 11a. The second end surface 12a is located at the end in the second direction (−x direction in the figure) opposite to the first direction, and the second opening 12b opens to the second end surface 12a.

The capillary 10 can be made of various known materials such as glass, resin, ceramics, and metal, but it is preferable that the liquid inside is transparent so that the liquid inside can be visually recognized. For example, resin or glass is suitable. Can be used for. The rough shape of the capillary 10 may be a cylindrical shape, and various shapes can be selected, but a cylindrical shape is preferable from the viewpoint of ease of manufacturing. The inner diameter of the capillary 10 can be appropriately set according to the amount of the liquid to be collected, and can be, for example, about 0.1 mm to 0.3 mm. The outer diameter of the capillary 10 can be appropriately set, and can be, for example, about 0.4 mm to 1.2 mm. The length of the capillary 10 can be appropriately set according to the amount of liquid to be sucked and stirred and the shape of the pipette to which the capillary is attached, and is set to, for example, about 20 mm to 100 mm.

For example, the portion of the capillary 10 to which the liquid adheres, such as the first end surface 11a of the capillary 10, the inner surface 13, and the + x direction side of the outer surface 14, may have water repellency. This makes it possible to accurately collect the amount of liquid and reduce unintended movement of the liquid in the capillary 10. When the material constituting the capillary 10 does not have water repellency, for example, a water repellent film can be formed on the surface of the capillary 10.

As the water-repellent film, various water-repellent films such as a water-repellent film formed by a silane coupling agent, a metal alkoxide-containing water-repellent film, a silicone-containing water-repellent film, or a fluorine-containing water-repellent film can be used. can. As a method for forming the water-repellent film on the surface of the capillary 10, various methods can be used. Examples of the dry process method include a physical vapor deposition method such as a physical vapor deposition method and a sputtering method, and a chemical vapor deposition method such as a chemical vapor deposition (CVD) method and an atomic layer deposition (ALD) method. Examples of the wet process method include a sol-gel method, a dip coating method, and a coating method.

The first end surface 11a is not perpendicular to the first direction (+ x direction in the figure), but is a plane inclined with respect to the first direction. This makes it possible to reduce the amount of liquid adhering to the first end surface 11a when the first end surface 11a side of the capillary 10 is brought into contact with the liquid and then pulled up. The angle formed by the first direction and the first end surface 11a can be appropriately set, for example, 40 ° or more and 80 ° or less, and for example, 60 ° or more and 70 ° or less.

Further, the first end surface 11a has a first end 11c located at an end in the first direction. The distance between the first end 11c and the first opening 11b is made larger than the distance between the portion of the first end surface 11a other than the first end 11c and the first opening 11b. The distance between the first end 11c and the first opening 11b is the distance between the portion of the first opening 11b closest to the first end 11c and the first end 11c, as shown by d1 in FIGS. 2 and 3. Means. The distance between the first end 11c and the first opening 11b may be, for example, a 0.2mm or 0.7mm or less, if example embodiment, may be 0.4mm or 0.6mm or less. Further, the distance between the first end 11c and the first opening 11b may be at least twice the passing distance of the first opening 11b. Further, the distance between the first end 11c and the first opening 11b may be at least twice the distance between the end in the second direction (-x direction in the figure) on the first end surface 11a and the first opening 11b. It is even better to double or more.

As described above, the capillary of the present disclosure has a cylindrical shape, and has a first end surface 11a, a second end surface 12a, a first opening 11b, and a second opening 12b. The first end surface 11a is located at the end in the first direction, and the first opening 11b is open to the first end surface 11a. The second end surface 12a is located at the end in the second direction opposite to the first direction, and the second opening 12b is open to the second end surface 12a. The first end surface 11a is inclined with respect to the first direction and has a first end 11c located at the end in the first direction. The distance between the first end 11c and the first opening 11b is larger than the distance between the portion of the first end surface 11a other than the first end 11c and the first opening 11b. This configuration is the basic configuration of the capillary of the present disclosure. The capillary of the present disclosure may have this basic configuration, and other configurations may be changed or omitted as appropriate. This basic configuration has the effect of reducing the occurrence of problems caused by the liquid adhering to the first end surface 11a entering the inside of the capillary, that is, the problem that the amount of liquid to be collected is slightly larger than the intended amount. Obtainable.

The mechanism by which this effect is obtained can be inferred as follows. That is, when the capillary 10 is pulled out from the liquid in the −x direction, the first end 11c located at the + x direction end of the capillary 10 is the place where the capillary 10 and the liquid finally separate from each other. Therefore, by increasing the distance between the first end 11c and the first opening 11b, it is possible to prevent the phenomenon that the liquid adhering to the first end surface 11a enters the inside of the capillary 10 due to surface tension. However, since an increase in the area of the first end surface 11a leads to an increase in the amount of liquid adhering to the first end surface 11a, the first end 11c and the first opening 11b are combined while suppressing the increase in the area of the first end surface 11a. By increasing the distance, it is possible to reduce the occurrence of the problem that the amount of liquid to be collected is larger than the intended amount.

Further, in the capillary of the present disclosure, the first end surface 11a may have a shape having one angle 11d, and the first end 11c may be the apex of the angle 11d. When having such a configuration, the above-mentioned effect can be enhanced. Further, the angle 11d may be an acute angle (the angle θ shown in FIG. 3 is an acute angle), and when such a configuration is provided, the effect can be further enhanced.

Further, the capillary 10 has a first portion 10a and a second portion 10b. The first portion 10a is relatively located on the first direction side and has a first end surface 11a and a first opening 11b. The second portion 10b is relatively located on the second direction side and has a second end surface 12a and a second opening 12b. The first portion 10a and the second portion 10b can be joined by various known joining methods such as joining and fitting with various adhesives.

The second portion 10b may have a simpler shape than the first portion 10a, and may have, for example, a cylindrical shape. The first portion 10a has the first end surface 11a and the first end 11c described above, and needs to have a more complicated shape than the second portion 10b. Therefore, by constructing the first portion 10a using a material that can easily form a complicated shape, for example, a material such as a resin, a capillary 10 that can be easily manufactured can be obtained. As the resin, for example, polypropylene, polyethylene, polytetrafluoroethylene and the like can be preferably used.

However, for example, when the liquid sucked into the capillary 10 is analyzed by using a fluorescent reaction, the fluorescent reaction of the resin itself makes it difficult to analyze the liquid. Therefore, for example, by constructing the second portion 10b, which can have a simple structure, using a material that does not exhibit a fluorescence reaction, for example, a material such as glass, the liquid in the second portion 10b is fluorescently reacted. Can be analyzed using.

As described above, the capillary of the present disclosure is located relatively on the first direction side, and is located relatively on the second direction side with the first portion 10a having the first end surface 11a and the first opening 11b. A second portion 10b having a second end surface 12a and a second opening 12b may be provided, and the first portion 10a and the second portion 10b may be made of different materials. With such a configuration, a highly functional and easy-to-manufacture capillary 10 can be obtained. Further, in the capillary of the present disclosure, the first portion 10a may be made of resin and the second portion 10b may be made of glass. With such a configuration, it is possible to obtain a capillary that is easy to analyze the fluorescence of the liquid in the capillary and is easy to manufacture.

Further, the capillary of the present disclosure may have a step at the boundary between the first portion 10a and the second portion 10b on the inner surface 13. When having such a configuration, when a plurality of liquids sucked into the capillary 10 are reciprocated in the capillary 10 to be mixed, turbulence can be generated to improve the mixing efficiency.

Further, the capillary of the present disclosure may have a plurality of grooves toward the first end 11c on the first end surface 11a. With such a configuration, the water repellency of the first end surface 11a can be enhanced, so that the above-mentioned effect can be further enhanced. The depth, width, and spacing of the grooves can be, for example, about several μm.

Further, the capillary of the present disclosure may have a plurality of grooves toward the first direction (+ x direction in the figure) on the outer surface 14. When having such a configuration, the outer surface 14
Since the water repellency of the above-mentioned can be enhanced, the above-mentioned effect can be further enhanced. The depth, width, and spacing of the grooves can be, for example, about several μm.

FIG. 4 is a cross-sectional view schematically showing a specific example of the pipette of the present disclosure. The pipette of this embodiment has a capillary 10, a pipette body 20, a first control unit 24, and a second control unit 25.

The pipette body 20 is configured by joining a piezoelectric substrate (piezoelectric actuator) 40, a first member 30, and a second member 60. The pipette body 20 has a pressure chamber 21, a first flow path 22, and a second flow path 26 inside. The pressure chamber 21 is a cavity provided inside the pipette body 20. In the pressure chamber 21, a part of the wall surrounding the pressure chamber 21 is deformable, and the volume of the pressure chamber 21 changes due to the deformation of the wall. The first flow path 22 and the second flow path 26 are air passages provided inside the pipette body 20, and are cavities having an elongated shape, respectively.

The first member 30 is a member constituting the side wall of the pressure chamber 21, and can be configured by using various materials such as metal, ceramic, and resin. The first member 30 can be, for example, in the shape of a plate having a thickness of about 50 μm to 5 mm, and a through hole serving as a pressure chamber 21 is formed in the center. The shape and size of the through hole can be appropriately selected, and for example, it can be a circular shape having a diameter of about 2 to 50 mm.

A second member 60 is joined to the first member 30 so as to close one opening of the through hole serving as the pressure chamber 21, and a part of the second member 60 is a part of the wall surrounding the pressure chamber 21. Consists of. The second member 60 has a first flow path 22 and a second flow path 26 inside. The second member 6 0 can be constructed using a metal, ceramic, various materials such as a resin.

The first flow path 22 extends in the + x direction. The + x direction end of the first flow path 22 is connected to the second opening 12b of the capillary 10. The first flow path 22 has an opening connected to the outside of the pipette at the end in the −x direction, and the opening is provided with a valve 23 for opening and closing the outside of the pipette and the first flow path 22. Has been done. The valve 23 opens and closes in response to a signal input from the outside. As the valve 23, various valves such as an electromagnetic valve, a piezoelectric valve, and an electrokinetic valve can be used. The shape and size of the first flow path 22 can be appropriately set, and for example, it can be a circular tube having a diameter of about 0.1 mm to 1 mm.

The second flow path 26 is connected to the first flow path 22 at a position between the end in the −x direction and the end in the + x direction in the first flow path 22, and is connected to the first flow path 22 from the portion connected to the first flow path 22. It extends in the + y direction. The shape and size of the second flow path 26 can be appropriately set, and for example, it can be a circular tube having a diameter of about 0.1 mm to 1 mm. From the viewpoint of reducing the inflow of liquid into the second flow path 26 due to the capillary phenomenon, it is preferable that the diameter of the second flow path 26 is larger than the diameter of the first flow path 22. The end of the second flow path 26 in the + y direction is connected to the pressure chamber 21.

A piezoelectric substrate 40 is joined to the first member 30 so as to close the other opening of the through hole serving as the pressure chamber 21, and a part of the piezoelectric substrate 40 constitutes a part of a wall surrounding the pressure chamber 21. doing. The piezoelectric substrate 40 has a flat plate shape having a size of about 3 mm □ to 100 mm □ and a thickness of about 20 μm to 2 mm, and has two laminated piezoelectric ceramic layers 40a and 40b. .. The thickness of the piezoelectric ceramic layers 40a and 40b can be, for example, about 10 μm to 30 μm. The piezoelectric ceramic layers 40a and 40b can be configured by using various piezoelectric materials. For example, having ferroelectricity, lead zirconate titanate (PZT), NaNbO ₃ system, KNaNbO ₃ system, BaTiO ₃ system, (BiNa) NbO ₃ system, the ceramic materials such as BiNaNb ₅ O ₁₅ system suitable Can be used for. The piezoelectric ceramic layer 40a is polarized in the thickness direction, and a voltage is applied to expand and contract in the horizontal direction. However, since no voltage is applied to the piezoelectric ceramic layer 40b, the piezoelectric ceramic layer 40b is piezoelectric. It may be made of a material other than the body.

Further, the piezoelectric substrate 40 has an internal electrode 42, a surface electrode 44, a connection electrode 46, and a through electrode 48. These electrodes and conductors can be constructed using a variety of metallic materials. A metal material such as Ag-Pd can be preferably used for the internal electrode 42 and the through electrode 48, and a metal material such as Au can be preferably used for the surface electrode 44 and the connection electrode 46.

The internal electrode 42 is arranged between the piezoelectric ceramic layer 40a and the piezoelectric ceramic layer 40b, and has substantially the same size as the piezoelectric substrate 40. The thickness of the internal electrode 42 can be, for example, about 2 μm.

The surface electrode 44 has a surface electrode main body 44a and a drawer electrode 44b, and is provided on the surface of the piezoelectric substrate 40. The surface electrode main body 44a has a planar shape substantially equal to that of the pressure chamber 21, and is provided so as to overlap the pressure chamber 21 in the thickness direction. The extraction electrode 44b is formed so as to be extracted from the surface electrode main body 44a. The thickness of the surface electrode 44 can be, for example, about 0.1 μm to 1 μm.

The connection electrode 46 is provided on the surface of the piezoelectric substrate 40, and is connected to the internal electrode 42 via a through electrode 48 penetrating the piezoelectric ceramic layer 40a.

A part of the piezoelectric ceramic layer 40a is sandwiched between the surface electrode main body 44a and the internal electrode 42. The drive unit 50 deformed by the application of a voltage is configured by the surface electrode main body 44a and the portions of the internal electrodes 42 and the piezoelectric ceramic layers 40a and 40b that overlap the surface electrode main body 44a in the thickness direction. As described above, the drive unit 50 is configured by using the piezoelectric body.

Then, by applying a voltage between the extraction electrode 44b and the connection electrode 46, the portion of the piezoelectric ceramic layer 40a sandwiched between the surface electrode body 44a and the internal electrode 42 expands or contracts in the horizontal direction, and the piezoelectric ceramic layer Since the 40b does not deform, the drive unit 50 bends. When the drive unit 50 is bent, the volume of the pressure chamber 21 is changed, the pressure of the second flow path 26 and the first passage 22 is varied, into the key Yapirari 10 connected to the first flow path 22 It is possible to suck the liquid and discharge the sucked liquid.

Note that FIG. 4 shows an example in which the piezoelectric actuator (piezoelectric substrate 40) constitutes a part of the wall of the pressure chamber 21, but the present invention is not limited to this. For example, an actuator of another type such as an electrostatic actuator may be used, and the wall of the pressure chamber 21 may be deformed by another method.

The first control unit 24 is electrically connected to the piezoelectric substrate 40, and changes the volume of the pressure chamber 21 by applying an electric signal to the piezoelectric substrate 40 to deform the piezoelectric substrate 40. As a result, the liquid can be sucked into the capillary 10 and the liquid can be discharged from the capillary 10. By driving the piezoelectric substrate 40 so that the volume of the pressure chamber 21 increases and decreases periodically, the liquid sucked into the capillary 10 can be vibrated and mixed.

The second control unit 25 is electrically connected to the valve 23, and opens and closes the valve 23 by giving an electric signal to the valve 23. When the liquid has flowed into the first flow path 22, the liquid can be discharged from the valve 23 to the outside by opening the valve 23. Further, after the piezoelectric substrate 40 is deformed and the liquid is sucked in, the valve 23 is opened, the deformation of the piezoelectric substrate 40 is restored in that state, the valve 23 is closed, and then the piezoelectric substrate 40 is deformed again. A large amount of liquid can be inhaled.

The first control unit 24 and the second control unit 25 can be configured by using various integrated circuits. The first control unit 24 and the second control unit 25 may be provided on the pipette body 20, but may not be the same. For example, a first control unit 24 and a second control unit 25 may be provided on a controller separate from the pipette body 20, and the pipette body 20 and the controller may be connected by a cable.

Next, an example of the operation of the pipette of this specific example will be described with reference to FIG. FIG. 5 is a graph schematically showing an example of a change in voltage in a signal output by the first control unit 24. In FIG. 5, the horizontal axis represents time and the vertical axis represents voltage.

First, the first signal 11 b of the first opening 11 b of the capillary 10 is immersed in the liquid A (not shown) , and at time t1, the first signal for driving the drive unit 50 so that the volume of the pressure chamber 21 increases is sent to the first control unit 24. There outputs, a predetermined voltage is marked addition to the piezoelectric ceramic layer 40a of the driving unit 50. As a result, the volume of the pressure chamber 21 increases, and the liquid A is sucked into the capillary 10.

Next, the first opening 11 b of the capillary 10 is immersed in the liquid B (not shown) , the first signal is output again by the first control unit 24 at time t2, and the first signal is output to the piezoelectric ceramic layer 40a of the drive unit 50 even higher. voltage is marked pressure. As a result, the volume of the pressure chamber 21 is further increased, and the liquid B is sucked into the capillary 10.

Next, the first opening 11 b of the capillary 10 is pulled out from the liquid B into the air, the first signal is output again by the first control unit 24 at time t3, and a higher voltage is applied to the piezoelectric ceramic layer 40a of the drive unit 50. is marked pressure. As a result, the volume of the pressure chamber 21 is further increased, and the liquid A and the liquid B move in the capillary 10 toward the second opening 12 b. This makes it possible to prevent the liquid from leaking to the outside of the capillary 10 when the liquid A and the liquid B are later reciprocated in the length direction of the capillary 10 and stirred.

Next, at time t4, the second control unit 25 outputs a third signal for opening the valve 23, whereby the valve 23 opens, the outside of the pipette and the pressure chamber 21 are connected, and the pressure inside the pressure chamber 21 is increased. Equal to atmospheric pressure. At this time, the positions of the liquid A and the liquid B do not change.

Next, at time t5, the first control unit 24 outputs a fifth signal for driving the drive unit 50 so that the volume of the pressure chamber 21 decreases, and the voltage applied to the piezoelectric ceramic layer 40a of the drive unit 50 becomes zero. Become. As a result, the volume of the pressure chamber 21 is reduced, but since the pressure chamber 21 is connected to the outside, the positions of the liquid A and the liquid B do not change.

Next, at time t6, the second control unit 25 outputs a fourth signal for closing the valve 23, whereby the valve 23 is closed and the pressure chamber 21 is cut off from the outside. At this time, the positions of the liquid A and the liquid B do not change.

Next, from time t6 to t8, the first control unit 24 outputs a second signal for driving the drive unit 50 so that the volume of the pressure chamber 21 increases or decreases periodically. As a result, the liquid A and the liquid B reciprocate in the length direction of the capillary 10 and are stirred, and the liquid A and the liquid B are mixed.

Although FIG. 5 shows an example in which a positive voltage is applied to the drive unit 50, for example, the direction of polarization of the piezoelectric ceramic layer 40a may be reversed to apply a negative voltage to the drive unit 50. I do not care.

Further, after the time t8, the first control unit 24 may output the first signal for increasing the volume of the pressure chamber 21 again. When having such a configuration, since the liquid A and the liquid B can be mixed and then moved to the second opening 12b side, the amount of the liquid located in the second portion 10b is increased, and the second portion is formed. It becomes easy to carry out the spectroscopic analysis in the two parts 10b.

As described above, the pipette of the present disclosure is a drive that changes the volume of the capillary 10 of the present disclosure, the pressure chamber 21 connected to the inside of the capillary 10 via the second opening 12b, and the pressure chamber 21. It has a part 50 and. This configuration is the basic configuration of the pipette of the present disclosure. The pipette of the present disclosure may have this basic configuration, and other configurations may be changed or omitted as appropriate. With this basic configuration, it is possible to obtain a pipette capable of collecting an accurate amount of liquid.

Further, in the pipette of the present disclosure, the drive unit 50 is configured by using a piezoelectric body, and in the second signal, the magnitude of the voltage changes periodically and the absolute value of the average value of the voltage in one cycle is used. It may be a signal that becomes smaller with the passage of time. When having such a configuration, when the liquid in the capillary 10 is reciprocated in the length direction of the capillary 10 and stirred, the liquid in the capillary 10 gradually moves to the second opening 12 of the capillary 10. It is possible to prevent the problem of moving to the b side. This problem was discovered by the inventors, and the cause has not yet been identified, but it has been confirmed by the inventors that this problem occurs even if the drive unit 50 that does not use a piezoelectric material is used. It can be solved by the pipette of the example.

Further, the pipette of the present disclosure may have a valve 23 that can be opened and closed to connect the outside and the pressure chamber 21. When having such a configuration, for example, by repeatedly sucking the liquid and opening and closing the valve 23, the volume of the liquid exceeding the increase in the volume of the pressure chamber 21 due to the drive of the drive unit 50 is sucked. Is possible.

Further, the pipette of the present disclosure has a second control unit 25 for controlling the valve 23, and before the first control unit 24 outputs the second signal, the third signal for opening the valve 23 and the valve 23 are displayed. The fourth signal to be closed is sequentially output from the second control unit 25, and the drive unit 50 is set so that the volume of the pressure chamber 21 is reduced between the time when the third signal is output and the time when the fourth signal is output. The fifth signal to be driven may be output from the first control unit 24. With such a configuration, for example, it is possible to prevent the reciprocating distance when the liquid in the capillary 10 is reciprocated from being reduced due to the limit of the amount of deformation of the drive unit 50. .. Further, it is possible to reduce the magnitude of the voltage marked addition to the driving unit 50.

10: Capillary 10a: First part 10b: Second part 11a: First end surface 11b: First opening 11c: First end 12a: Second end surface 12b: Second opening 21: Pressure chamber 50: Drive unit

Claims (6)

In the first end surface located at the end in the first direction, the first opening opened in the first end surface, the second end surface located in the end in the second direction opposite to the first direction, and the second end surface. It has a tubular shape with a second opening to open, and has a tubular shape.
The first end surface is inclined with respect to the first direction.
The first end face has a first end located at the end in the first direction.
The distance between the first end and the portion of the first opening closest to the first end is the largest in the portion other than the first end in the first end surface and the portion other than the first end in the first opening . much larger than the distance between the near portion, and,
It is located relatively on the first direction side, and is located relatively on the second direction side with the first portion having the first end surface and the first opening for contact with the liquid. A capillary having the second end face and the second opening, and a second portion for analyzing an internal liquid using a fluorescence reaction . The capillary according to claim 1, wherein the first end surface has a shape having one angle, and the first end is an apex of the angle. The capillary according to claim 2, wherein the angle is an acute angle. Capillary according to any one of claims 1 to 3, characterized in that the front Symbol first portion and said second portion is configured mutually different materials. The capillary according to claim 4, wherein the first portion is made of resin and the second part is made of glass. The capillary according to any one of claims 1 to 5 and
A pressure chamber connected to the inside of the capillary through the second opening,
A drive unit that changes the volume of the pressure chamber,
A pipette characterized by having.

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