JP6993740B2 - Thread and its manufacturing method - Google Patents

Description

The present invention relates to a yarn and a method for producing the same.

In the field of regenerative medicine, there is an urgent need for technological development to form the framework of tissues and organs by knitting filamentous materials that function as scaffolds for cells.

The present inventor has developed a technique for processing a columnar native collagen gel into a thread, and then aims to put it into practical use as a tissue regenerating thread (suture thread, indwelling thread, cell transplant thread, etc.) in the field of regenerative medicine. Therefore, we have developed a technique for processing a columnar atelocollagen gel irradiated with UV into a thread shape (see, for example, Patent Documents 1 and 2).

Japanese Patent No. 467559 International Publication No. 2018/21187

Based on the above development results, the present inventor has aimed to develop a filamentous atelocollagen vitrigel having further excellent practicality.

The present invention has been made in view of the above circumstances, and provides an easy manufacturing technique for an infinite length filamentous atelocollagen vitrigel and a dried product thereof.

The present invention includes the following aspects.
[1] A method for producing a yarn, which comprises a step of twisting a first plate-shaped hydrogel dried product or a plate-shaped Vitrigel dried product while moistening it with the first aqueous solution to form a thread-like vitrigel.
[2] The end of the first plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body left without being threaded is overlapped with the end of the second plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body. Together, the joint is formed, and the joint and the second plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body are twisted while being moistened with the first aqueous solution to form a thread-like vitrigel. The method for producing a yarn according to [1], which comprises a step of obtaining the yarn.
[3] The method for producing a yarn according to [1] or [2], which comprises a step of drying the filamentous Vitrigel to obtain a dried filamentous Vitrigel.
[4] The method for producing a yarn according to [3], which comprises a step of moistening the dried filamentous Vitrigel with a second aqueous solution and then drying the dried yarn.
[5] The method for producing a yarn according to [3] or [4], which comprises a step of irradiating the dried filamentous Vitrigel with ultraviolet rays.
[6] The method for producing a thread according to any one of [1] to [5], wherein the hydrogel is an atelocollagen gel.
[7] The method for producing a yarn according to any one of [1] to [6], wherein the first aqueous solution is water or an atelocollagen sol.
[8] The method for producing a yarn according to any one of [2] to [6], wherein the end portion is a protrusion.
[9] The method for producing a yarn according to any one of [4] to [8], wherein the second aqueous solution is an atelocollagen sol.
[10] A step of injecting a sol into a mold, gelling the sol, and then removing the mold to obtain a plate-shaped hydrogel, and drying and vitrifying the plate-shaped hydrogel to obtain a plate-shaped hydrogel dried product. The method for producing a yarn according to any one of [1] to [9], which comprises a step.
[11] The method for producing a yarn according to [10], which comprises a step of irradiating the dried plate-shaped hydrogel body with ultraviolet rays.
[12] A step of rehydrating the plate-shaped hydrogel dried product or the plate-shaped hydrogel dried product irradiated with ultraviolet rays to obtain a plate-shaped Vitrigel, and drying and revitrifying the plate-shaped Vitrigel to obtain a plate-shaped Vitrigel. The method for producing a thread according to [10] or [11], which comprises a step of obtaining a dried body.
[13] The method for producing a yarn according to [12], which comprises a step of irradiating the dried plate-shaped Vitrigel with ultraviolet rays.
[14] A thread made of a dried Vitrigel and having elasticity when hydrated.
[15] The thread according to [14], which has a spiral structure.
[16] The thread according to [14] or [15], wherein the dried Vitrigel is an atelocollagen dried Vitrigel.

According to the present invention, it is possible to provide an easy manufacturing technique for an infinite length filamentous atelocollagen vitrigel and a dried product thereof.

This is an example of a plate-shaped Vitrigel dried product used in the present embodiment. This is an example of a manufacturing process of the plate-shaped Vitrigel dried product used in the present embodiment. It is an example of the manufacturing method of the yarn of this embodiment. It is an example of the manufacturing method of the yarn of this embodiment. It is a photograph of the yarn manufactured in Production Example 1. It is a test result in Example 1. It is a test result in Example 1. It is a test result in Example 1. It is a test result in Example 1.

Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings as the case may be. The dimensional ratio in each figure is exaggerated for the sake of explanation and does not necessarily match the actual dimensional ratio.

≪Yarn manufacturing method≫
In one embodiment, the present invention comprises a step E of obtaining a thread-like vitrigel by twisting the first plate-shaped hydrogel dried body or the plate-shaped vitrigel dried body while moistening it with the first aqueous solution to form a thread. , Provide a method for manufacturing a yarn.

First, a method for producing a plate-shaped hydrogel dried body will be described.
The method for producing a dried plate-shaped hydrogel is a step A of injecting a sol into a mold, gelling the sol, and then removing the mold to obtain a plate-shaped hydrogel, and drying and vitrifying the plate-shaped hydrogel. , A step B for obtaining a plate-shaped hydrogel dried sol.

FIG. 1 shows an example of a plate-shaped hydrogel dried body. As shown in FIG. 1, it is preferable that the end of the dried plate-shaped hydrogel is a protrusion for ease of twisting. Further, in FIG. 1, the plate-shaped hydrogel dried body has a strip shape and a narrow width, but the width is not limited to this and can be appropriately adjusted. The wider the width of the plate-shaped hydrogel dried body, the thicker the thread, and the narrower the width of the plate-shaped hydrogel dried body, the thinner the thread. In this way, the thickness of the yarn can be controlled by adjusting the width of the plate-shaped hydrogel dried body.
Further, the width of the plate-shaped hydrogel dried body does not have to be uniform, the width may be appropriately different as in the gourd type, and the width may be gradually different as in the triangular shape. May be. Further, the dried plate-shaped hydrogel may be a perforated, for example, a dried porous plate-shaped hydrogel.

[Step A]
Step A is a step of injecting the sol into the mold, gelling the sol, and then removing the mold to obtain a plate-shaped hydrogel (see FIGS. 2 (a) to 2 (d)).
The mold is not particularly limited as long as the desired plate-shaped hydrogel shape is hollowed out, and PET in which a plurality of plate-shaped hydrogel shapes are hollowed out, for example, as shown in FIG. 2A. The film is mentioned.
As used herein, the term "sol" means that the dispersoidal colloidal particles (size: about 1 to several hundred nm) using a liquid as a dispersion medium are particularly composed of a polymer compound. More specific examples of the sol include an aqueous solution of a natural product polymer compound or a synthetic polymer compound. When these polymer compounds are crosslinked by chemical bonds to form a network structure, they are transferred to "hydrogel", which is a semi-solid substance having a large amount of water in the network. That is, "hydrogel" means a gelled sol.

The sol used as a raw material for the plate-shaped hydrogel may be any material having biocompatibility, for example, gelling extracellular matrix-derived components, natural polymer compounds such as fibrin, agar, agarose, and cellulose, and poly. Examples thereof include synthetic polymer compounds such as acrylamide, polyvinyl alcohol, polyethylene oxide, and poly (II-hydroxlythylmethacrylate) / polycaprolactone.

Examples of the extracellular matrix-derived component that gels include collagen (type I, type II, type III, type V, type XI, etc.), mouse EHS tumor extract (type IV collagen, laminin, heparan sulfate proteoglycan, etc.). ), Basement membrane components (trade name: Matrigel), glycosaminoglycan, hyaluronic acid, proteoglycan, gelatin and the like, and are not limited thereto. It is possible to produce a desired plate-shaped hydrogel by selecting a component such as a salt most suitable for each gelation, its concentration, pH and the like. In addition, by combining raw materials, it is possible to obtain a plate-shaped hydrogel that imitates various in-vivo tissues.

Among them, as the sol, an extracellular matrix-derived component that gels is preferable, and collagen is more preferable. Further, as a more preferable raw material among collagens, native collagen or atelocollagen can be exemplified, and atelocollagen is further preferable.

The term "vitrigel" refers to a gel in a stable state obtained by rehydration of a conventional hydrogel after vitrification, and is "vitrigel" (registered) by the present inventor. It is named "Trademark)".
Further, in the present specification, in explaining the manufacturing process of the present embodiment in detail, the dried hydrogel immediately after the vitrification step and not undergoing the rehydration step is simply referred to as “hydro”. It was called "gel dried product". Then, the gel obtained through the rehydration step after the vitrification step was distinguished and represented as "Vitrigel", and the dried product obtained by vitrifying the Vitrigel was referred to as "Vitrigel dried product". Further, what was obtained by subjecting the dried Vitrigel body to a step of irradiating it with ultraviolet rays was referred to as a "dried Vitrigel body irradiated with ultraviolet rays". Therefore, "Vitrigel" is a hydrate.
Further, in the present specification, when the term "Vitrigel" is used, the term "(registered trademark)" may be omitted.

In step A, when the sol is injected into the mold, if the amount of the sol is increased, a thick plate-like hydrogel can be obtained, and as a result, a thick thread can be obtained. Further, if the amount of sol is reduced, a thin plate-shaped hydrogel can be obtained, and as a result, fine threads can be obtained. In this way, the thickness of the yarn can be controlled by adjusting the amount of the sol to be injected.
The thickness of the plate-shaped hydrogel is preferably 0.1 mm to 20 mm, more preferably 0.5 mm to 4 mm, still more preferably 1 mm to 2 mm.

In step A, the temperature at which the sol is kept warm during gelation may be appropriately adjusted according to the type of sol to be used. For example, when the sol is a collagen sol, the heat retention during gelation may be a temperature lower than the denaturation temperature of collagen depending on the animal species of collagen used, and is generally a temperature of 20 ° C. or higher and 37 ° C. or lower. By keeping warm in, gelation can be performed in a few minutes to a few hours.

[Step B]
Step B is a step of drying and vitrifying the plate-shaped hydrogel to obtain a plate-shaped hydrogel dried product (see FIG. 2 (e)). By drying the plate-shaped hydrogel, the free water in the plate-shaped hydrogel can be completely removed, and the partial removal of the bound water can be further promoted.
The longer the period of this vitrification step (the step of completely removing the free water in the plate-shaped hydrogel and then proceeding with the partial removal of the bound water), the better the transparency and strength when rehydrated. A plate-shaped vitrigel can be obtained. If necessary, the plate-shaped vitrigel obtained by rehydration after short-term vitrification can be washed with PBS or the like and vitrified again.

As a drying method, various methods can be used, for example, air drying, drying in a closed container (circulating the air in the container and constantly supplying dry air), drying in an environment in which silica gel is placed, and the like. .. For example, as an air-drying method, a method of drying in an incubator kept sterile at 10 ° C. and 40% humidity for 2 days, or drying in a sterile clean bench all day and night at room temperature can be exemplified. can.
Further, by irradiating the dried plate-shaped hydrogel with ultraviolet rays, it is possible to obtain a plate-shaped vitrigel having excellent transparency and strength when rehydrated.

Subsequently, a method for producing a plate-shaped Vitrigel dried product will be described.
The method for producing the plate-shaped hydrogel dried product is a step C of rehydrating the plate-shaped hydrogel dried product or the plate-shaped hydrogel dried product irradiated with ultraviolet rays to obtain a plate-shaped vitrigel, and drying the plate-shaped vitrigel. It has a step D, which revitrifies and obtains a plate-shaped Vitrigel dried product.
[Process C]
Step C is a step of rehydration after vitrification (see FIGS. 2 (f) to (g)). By step C, a plate-shaped Vitrigel is obtained.
Examples of the aqueous solution used for rehydration include sterile water, physiological saline, PBS and the like.

[Step D]
Step D is a step of drying and revitrifying the plate-shaped Vitrigel to obtain a plate-shaped Vitrigel dried product. Further, by irradiating the dried plate-shaped Vitrigel body with ultraviolet rays, the strength of the dried plate-shaped Vitrigel body can be increased (see FIG. 2 (h)).
The above-mentioned ultraviolet irradiation energy may be appropriately adjusted according to the composition and content of the plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body. The irradiation energy of ultraviolet rays may be, for example, 0.1 mJ / cm ² or more and 6000 mJ / cm ² or less, for example, 10 mJ / cm ² or more and 4000 mJ / cm ² or less, for example 20 mJ / cm ² or more and 500 mJ / cm ² . It may be as follows.

A yarn is produced using the plate-shaped hydrogel dried product or the plate-shaped Vitrigel dried product thus produced. The plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body may be hollowed out using a cylindrical blade or the like to form a through hole. When a yarn is produced using a plate-shaped hydrogel dried product having such through holes, the yarn having fine pores can be obtained. Examples of the cylindrical blade include a medical device such as a trepan. The shape of the blade may be appropriately changed according to the cross-sectional shape of the through hole, and is not limited to the cylindrical shape.

[Step E]
Step E is a step of obtaining a filamentous Vitrigel by twisting the first plate-shaped hydrogel dried product or the plate-shaped Vitrigel dried product while moistening it with the first aqueous solution to form a thread.
More specifically, as shown in FIG. 3, the obtained plate-shaped hydrogel dried product or plate-shaped Vitrigel dried product is cut in half in a horizontal direction, and one of the two halves is used as the first plate-shaped hydrogel. It is used as a dried body or a plate-shaped Vitrigel dried body (the rest is used as a second plate-shaped hydrogel dried body or a plate-shaped Vitrigel dried body in step F). First, the end of the first plate-shaped hydrogel dried body or plate-shaped Vitrigel dried body without protrusions is fixed to a support such as a pipette. Next, the fixed first plate-shaped hydrogel dried body or plate-shaped Vitrigel dried body is twisted in the same direction while being moistened with the first aqueous solution to form a thread (FIGS. 4A to 4B). )reference.).
The first aqueous solution is not particularly limited, and examples thereof include sterile water, physiological saline, PBS, and atelocollagen sol, and sterile water and atelocollagen sol are preferable.

When it is desired to make the filamentous vitrigel longer, it is preferable that the production method of the present embodiment has a step F.

[Step F]
In step F, the end portion of the first plate-shaped hydrogel dried product or the plate-shaped Vitrigel dried product and the end portion of the second plate-shaped hydrogel dried product or the plate-shaped Vitrigel dried product left without being threaded are formed. The joint portion is formed by superimposing the joint portion, and the joint portion and the second plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body are twisted while being moistened with the first aqueous solution to form a thread-like vitrigel. Is the process of obtaining.
More specifically, as shown in FIG. 3, the protrusions of the first plate-shaped hydrogel dried body or plate-shaped Vitrigel dried body left without being threaded, and the second plate-shaped hydrogel dried body or plate. A joint is formed by superimposing the protrusions of the dry Vitrigel (see FIGS. 4 (c) to 4 (d)). Then, while moistening with the first aqueous solution, the joint and the lower end of the second plate-shaped hydrogel dried body or the plate-shaped Vitrigel dried body are moistened and twisted in the same direction to form a thread (FIG. 4 (FIG. 4). e) See (g).).
By repeating step F, an infinite length yarn can be manufactured. Further, the manufacturing method of the embodiment may include the following steps GI.

[Process G]
Step G is a step of drying the filamentous Vitrigel obtained in Step F to obtain a dried filamentous Vitrigel (see FIG. 4 (h)).

[Step H]
Step H is a step of moistening the filamentous Vitrigel dried product obtained in Step G with a second aqueous solution and then drying the dried product.
The second aqueous solution is not particularly limited, and examples thereof include sterile water, physiological saline, PBS, and atelocollagen sol, and atelocollagen sol is preferable. By coating the dried filamentous Vitrigel with atelocollagen sol, the strength of the yarn can be increased.

[Step I]
Step I is a step of irradiating the dried filamentous Vitrigel obtained in Step H or G with ultraviolet rays.
The intensity of ultraviolet rays is the same as that listed in step D. In order to uniformly irradiate the dried filamentous Vitrigel with ultraviolet rays, it is preferable to irradiate the dried product in a symmetrical direction multiple times.
By irradiating the dried filamentous Vitrigel with ultraviolet rays, a crosslinked structure can be formed in the molecule and the strength of the yarn can be increased.

<Use>
The thread obtained by the production method of the present embodiment can be used, for example, as a tissue regeneration thread, a carrier for cell transplantation, or the like. As described above, according to the present embodiment, since an infinite length yarn can be produced, the yarn can be knitted into an organ shape and can be suitably used as a carrier for cell transplantation.

≪Thread≫
In one embodiment, the present invention provides a thread comprising a dried Vitrigel and having elasticity when hydrated. In the manufacturing method described above, the yarn has elasticity by having a twisting step. Further, by having a twisting process, the yarn has a spiral structure.
Examples of the sol as a raw material for the dried Vitrigel include the same as those exemplified in the above-mentioned method for producing a yarn. Among them, the dried Vitrigel body constituting the thread of the present embodiment is preferably a dried Atelocollagen Vitrigel because it is a biocompatible material.

Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited to the following examples.

[Manufacturing Example 1] Manufacture of yarn 1. Place a mold of 8 strips of 10 mm x 200 mm (cut 2.5 mm x 20 mm at both ends: see Fig. 1) on an A4 size PET film (thickness 75 μm) on a Teflon (registered trademark) plate. (See FIG. 2 (a)).
2. 2. 8 mL of 1% atelocollagen solution was poured into 8 mL of serum-free culture medium on ice, and pipetting was performed 3 times to prepare a uniform atelocollagen sol.
3. 3. 3.6 mL of atelocollagen sol was poured into the inside of each strip-shaped mold so as to spread throughout (4 places).
4. The above steps 2 and 3 were repeated, and the atelocollagen sol was poured into a total of 8 molds (see FIG. 2 (b)).
A template infused with atelocollagen sol was left for 2 hours in a 5% CO ₂ incubator at 5.37 ° C. to gel (see FIG. 2 (c)).
6. After gelation, the PET film was removed (see FIG. 2 (d)) and vitrified in a constant temperature and humidity chamber with a humidity of 40% and a temperature of 10 ° C. (see FIG. 2 (e)).
7. After vitrification, sterile water was added, washed 3 times, and rehydrated (see FIG. 2 (f)). After rehydration (see FIG. 2 (g)), revitrification was performed in a constant temperature and humidity chamber with a humidity of 40% and a temperature of 10 ° C.
8. After revitrification, the dried atelocollagen Vitrigel membrane was irradiated with UV at 50 mJ / cm ² (see FIG. 2 (h)).

9. After UV irradiation, strip-shaped Atelocollagen Vitrigel membrane dried product was peeled off from the Teflon (registered trademark) plate, cut in half, and attached to a pipette with one end prepared and hung with scotch mending tape (Fig. 3). , See FIG. 4 (a)).
10. The strip-shaped atelocollagen Vitrigel membrane dried product was moistened with sterile water and twisted in the same direction to form a thread. The tip (5 mm × 20 mm portion) of the strip was left without being threaded (see FIG. 4 (b)).
11. Overlay the tip (5 mm x 20 mm) of another strip-shaped atelocollagen Vitrigel membrane dried body on the remaining lower end (see FIGS. 3, 4 (c) to (d)), and moisten it with sterile water. Twisted in the same direction. Subsequently, the clips were twisted in the same direction while being moistened with sterile water to the lower end (see FIGS. 4 (e) to 4 (h)), and then the clips were made into heavy stones and dried.
12. By repeating the above operations 9 to 11, four filamentous atelocollagen Vitrigel dried products (twisting method: sterile water only) were prepared.
13. Two of the four prepared were twisted in the same direction while being further moistened with atelocollagen sol, and then the clips were made into heavy stones and dried (twisting method: sterile water + atelocollagen sol coat).
14. The above 10 and 11 were newly carried out using atelocollagen sol instead of sterile water to prepare two filamentous atelocollagen vitrigel dried products (twisting method: atelocollagen sol).
15. Each one prepared in 12 to 14 above was irradiated with 400 mJ / cm ² UV twice in the symmetrical direction.

[Example 1] Strength confirmation test of the dried filamentous atelocollagen Vitrigel Each sample of the dried filamentous atelocollagen Vitrigel produced in Production Example 1 was placed in a 50 mL conical tube containing sterile water and rehydrated. One day after rehydration, each sample was taken out and both ends of the thread were pulled to observe the degree of unraveling (see FIG. 6). Each sample has the following six types. (1) -1: Yarn (twisting method: sterilized water), (1) -2: UV irradiation sample of yarn (twisting method: sterilized water), (2) -1: Yarn (twisting method: sterilized water) + Atelocollagen sol coat), (2) -2: UV irradiation sample of yarn (twisting method: sterilized water + atelocollagen solcoat), (3) -1: Thread (twisting method: atelocollagen sol), (3)- 2: Sample by UV irradiation of yarn (twisting method: atelocollagen sol) (see FIG. 5).

The results are shown in FIGS. 7-9.
As shown in FIG. 7 (a), in the yarn produced by twisting with sterile water, a large unraveling was observed, and when both ends were pulled, the yarn was cut at the joint. As shown in FIG. 7 (b), in the yarn produced by twisting with sterile water and then irradiating with UV, moderate unraveling was observed, and when both ends were pulled, the yarn was cut at the joint, but its strength was UV. It was stronger than the one without irradiation.

As shown in FIG. 8A, in the yarn produced by twisting with sterile water and then atelocollagen sol coating, a large unraveling was observed, and when both ends were pulled, the yarn was cut at the joint, but its strength was sterilized. It was stronger than the yarn made only by twisting with water. As shown in FIG. 8 (b), in the yarn produced by twisting with sterile water, coating with atelocollagen sol, and irradiating with UV, moderate unraveling was observed, and when both ends were pulled, it was cut at the joint. However, its intensity was stronger than that without UV irradiation.

As shown in FIG. 9 (a), in the yarn produced by twisting with atelocollagen sol, a slight unraveling was observed, and when both ends were pulled, the yarn was cut at the joint, but its strength was twisted with sterile water. Threads made by sterilizing water, threads made by UV irradiation after twisting with sterilized water, threads made by twisting with sterilized water and then with atelocollagen sol coating, and twisting with sterilized water and then atelocollagen. It was stronger than the yarn produced by sol coating and UV irradiation. As shown in FIG. 9B, the yarn produced by twisting with atelocollagen sol and then irradiating with UV does not unravel, has elasticity like rubber, and does not easily break even if both ends are pulled. Its intensity was stronger than that without UV irradiation.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a technique for easily producing an infinite length filamentous atelocollagen vitrigel whose strength can be adjusted according to an application and a dried product thereof.

Claims (15)

A method for producing a yarn, comprising a step of obtaining a thread-like vitrigel by twisting a first plate-shaped hydrogel dried body or a film-shaped vitrigel dried body while moistening it with the first aqueous solution to form a thread. The end of the first plate-shaped hydrogel dried body or the film-shaped Vitrigel dried body left without being threaded and the end of the second plate-shaped hydrogel dried body or the film-shaped Vitrigel dried body are overlapped and joined. A step of forming a portion and twisting the joint portion and the second plate-shaped hydrogel dried body or plate-shaped Vitrigel dried body while moistening with the first aqueous solution to form a thread-like vitrigel is obtained. The method for producing a thread according to claim 1. The method for producing a yarn according to claim 1 or 2, further comprising a step of drying the filamentous Vitrigel to obtain a dried filamentous Vitrigel. The method for producing a yarn according to claim 3, further comprising a step of moistening the dried filamentous Vitrigel with a second aqueous solution and then drying the dried yarn. The method for producing a yarn according to claim 3 or 4, which comprises a step of irradiating the dried filamentous Vitrigel with ultraviolet rays. The method for producing a yarn according to any one of claims 1 to 5, wherein the hydrogel is a native collagen gel. The method for producing a yarn according to any one of claims 1 to 6, wherein the first aqueous solution is water, a native collagen sol or an atelocollagen sol. The method for manufacturing a yarn according to any one of claims 2 to 6, wherein the end portion is a protrusion. The method for producing a yarn according to any one of claims 4 to 8, wherein the second aqueous solution is a native collagen sol or an atelocollagen sol. The process of injecting the sol into the mold, gelling the sol, and then removing the mold to obtain a plate-shaped hydrogel,
The step of drying and vitrifying the plate-shaped hydrogel to obtain a plate-shaped hydrogel dried product, and
The method for producing a yarn according to any one of claims 1 to 9. The method for producing a yarn according to claim 10, further comprising a step of irradiating the dried plate-shaped hydrogel with ultraviolet rays. The step of rehydrating the plate-shaped hydrogel dried product or the plate-shaped hydrogel dried product irradiated with ultraviolet rays to obtain a film-shaped Vitrigel, and drying and re-glazing the film-shaped Vitrigel to obtain a film-shaped Vitrigel dried product. The method for producing a yarn according to claim 10 or 11, further comprising a step of obtaining the yarn. The method for producing a yarn according to claim 12, further comprising a step of irradiating the dried film-like Vitrigel with ultraviolet rays. Vitrigel A thread made of dried material, having a spiral structure, and having elasticity when hydrated. The thread according to claim 14, wherein the dried Vitrigel is a dry native collagen Vitrigel.

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