JP7094537B2 - Yuzu (Yuzu) -containing composition - Google Patents

Description

The present invention relates to a composition containing Yukou or an extract thereof as an active ingredient. The present invention is also useful as an antibacterial composition, an intestinal regulating composition, a composition for preventing / ameliorating periodontal disease and / or halitosis, and a composition for preventing / ameliorating diabetes. It may also be useful as food and drink, cosmetics, personal care products and home care products, including.

Yuko (Citrus Yuko) is a citrus citrus fruit of the Rutaceae family, a natural hybrid of Yuzu and Daidai , and is known as the original species of Sudachi. Yuzu has poor cold resistance compared to Yuzu and Sudachi, and its production is limited to a specific area centered on Tokushima Prefecture, so its production is low and its name is low nationwide. Therefore, little nutritional and food science research on Yukou has been conducted so far.

Although the composition and active ingredients of the natural material Yuzu have not been clearly elucidated, it is believed that Yuzu has characteristics not found in other citrus fruits such as Yuzu and Sudachi. Therefore, it is considered that Yukou has a different use from other citrus fruits.

It has been reported that Yukou can be used as a hair restorer and cosmetics, but there is no particular report on other uses except that it is used for component comparison with other citrus fruits (Non-Patent Document 1). -2 and Patent Documents 1-2).

Japanese Unexamined Patent Publication No. 07-126129 Japanese Unexamined Patent Publication No. 07-126142

Yoshiaki Miyake, Journal of the Japanese Society of Food Life, Vol. 26, No. 2, 71-78 (2015) Masao Tsukazan et al., Journal of Japanese Food Science and Engineering, Vol. 56, No. 6, 359-362 (2009)

The present invention finds new uses for Yukou, which is not well known nationwide and is mostly treated as waste, and provides compositions that can be used in a wide range of fields such as pharmaceuticals, foods and drinks, cosmetics, and daily necessities. The purpose is.

As a result of diligent studies to solve the above problems, the present inventors have discovered that Yukou, particularly Yukou juice, has antibacterial activity, and as an accompanying effect,
(1) Improve the disorder of the intestinal flora caused by a high-fat diet, specifically, decrease lactic acid bacteria and bifidobacteria, which are said to be good bacteria, and increase Clostridium, which is said to be bad bacteria. To improve the intestinal flora,
(2) Increases Akkermansia, which has anti-diabetes and anti-obesity effects.
It has been found that it has various excellent effects that can be practically applied, such as (3) suppressing the growth of oral bacteria and the concentration of volatile sulfide in the oral cavity, and (4) improving impaired glucose tolerance induced by a high-fat diet. , The present invention has been completed.

That is, the present invention provides the following aspects.
[1] An antibacterial composition containing Yukou or an extract thereof as an active ingredient.
[2] The antibacterial composition according to [1], wherein the yukou or an extract thereof is a fruit juice, a peel, a peel extract or a fruit juice squeezed extract of the yukou.
[3] The antibacterial composition according to [2], wherein the fruit juice is a juice obtained by squeezing a fruit (raw juice), a juice obtained by diluting the raw juice, a concentrated juice, or a concentrated reduced fruit juice.
[4] An intestinal regulating composition containing Yukou or an extract thereof as an active ingredient.
[5] The composition for intestinal regulation according to [4], which improves the deterioration of the intestinal bacterial flora due to a high-fat diet.
[6] The composition for intestinal regulation according to [4] or [5], which is characterized by increasing Akkermansia in the intestine.
[7] A composition for preventing and / or ameliorating periodontal disease and / or halitosis, which comprises Yukou or an extract thereof as an active ingredient.
[8] A composition for preventing and / or ameliorating diabetes, which comprises Yukou or an extract thereof as an active ingredient.
[9] The composition for preventing and / or ameliorating diabetes according to [8], which is characterized by ameliorating impaired glucose tolerance induced by a high-fat diet.

[10] A food composition containing Yukou or an extract thereof as an active ingredient.
[11] The food composition according to [10], wherein the yukou or an extract thereof is a yukou fruit juice, a pericarp, a pericarp extract or a fruit juice squeezed slag extract.
[12] The food composition according to [11], wherein the fruit juice is a juice obtained by squeezing a fruit (raw juice), a juice obtained by diluting the raw juice, a concentrated juice, or a concentrated reduced fruit juice.
[13] The food composition according to any one of [10] to [12], for improving the deterioration of the intestinal bacterial flora due to a high-fat diet.
[14] The food composition according to [13], which is characterized by increasing Akkermansia in the intestine.
[15] The food composition according to any one of [10] to [12] for preventing and / or ameliorating periodontal disease and / or bad breath.
[16] The food composition according to any one of [10] to [12] for preventing and / or ameliorating diabetes.
[17] The food composition according to [16], which is characterized by improving high-fat diet-induced glucose intolerance.

Furthermore, the present invention provides the following aspects.
[18] A food or drink, a cosmetic product, a personal care product or a household care product containing the antibacterial composition according to any one of [1] to [3].
[19] A method for preserving foods and drinks, which comprises blending the food and drink with the antibacterial composition according to any one of [1] to [3].
[20] A method for preserving cosmetics, which comprises blending the antibacterial composition according to any one of [1] to [3] into cosmetics.
[21] A method for improving deterioration of the intestinal flora due to a high-fat diet, which comprises administering a therapeutically effective amount of Yukou or an extract thereof to a patient in need of treatment.
[22] A method for treating periodontal disease and / or halitosis, which comprises administering a therapeutically effective amount of Yukou or an extract thereof to a patient in need of treatment.
[23] A method for treating diabetes, which comprises administering a therapeutically effective amount of Yukou or an extract thereof to a patient in need of treatment.
[24] Use of Yukou or an extract thereof in the manufacture of a pharmaceutical to improve the deterioration of the intestinal flora due to a high-fat diet.
[25] Use of Yukou or its extracts in the manufacture of pharmaceuticals for the prevention and / or treatment of periodontal disease and / or halitosis.
[26] Use of Yukou or its extracts in the manufacture of pharmaceuticals to prevent and / or treat diabetes.

According to the present invention, since Yukou or an extract thereof effectively suppresses the growth of bacteria and fungi such as Escherichia coli and Staphylococcus aureus, it can be used for pharmaceuticals, foods, household care products and the like utilizing its antibacterial action. In addition, the effects associated therewith, that is, the effects of intestinal regulation, periodontal disease and / or prevention and / or improvement of mouth odor, prevention and / or improvement of diabetes, to various pharmaceuticals, foods, etc. You can expect to use it. In particular, since Yukou is a fruit of natural material, it is highly safe and widely applicable.

FIG. 1 shows each group (normal diet + 100% yukou juice administration group, normal diet + physiological saline administration group, high-fat diet (HFD-60%) + 100% yukou juice administration group, high-fat diet + saline solution). The composition of the intestinal flora in the administration group) is shown. FIG. 2 shows (A) blocking circles for Escherichia coli strain K12 (30%, 60% and 100% yuzu juice), and (B) blocking circles for yellow staphylococcus (100% yuko juice, 100% sudachi juice and yuzu juice). 100%), (C) Blocking circles (100% yuzu juice and 100% yuzu juice) for bread yeast (fungus) are shown. FIG. 3 shows (A) the number of E. coli colonies formed in various concentrations of Yukou juice, benzoic acid and acetic acid, and (B) the number of colonies formed by Alicyclobacillus acidteresteris in various concentrations of Yukou juice, benzoic acid and acetic acid. Is shown. FIG. 4 shows (A) the number of oral bacteria (number of periodontal disease bacteria) before and after ingestion of Yukou fruit juice, and (B) human β, which is an antibacterial peptide before and after ingestion of Yukou fruit juice. -Indicates the amount of defensin 1 (pg / ml). FIG. 5 shows the ratio of the number of bacteria after addition of Yukou soap solution (0.1 g / 100 μL) when the normal number of bacteria is 100%. FIG. 6 shows the blood glucose levels in the Yukou-administered group and the physiological saline-administered group (control group) after 0 to 120 minutes of glucose loading.

Hereinafter, the present invention will be described in detail.

In the present invention, "Yuko" refers to fruit juice, pericarp or lyophilized powder thereof derived from Citrus Yuko (Yuzuka). Juice, pericarp or freeze-dried powders thereof can be appropriately used depending on the intended purpose.

The juice of Yukou is not particularly limited as long as it is a juice obtained from Yukou. Further, the juice of Yukou may be diluted with a solvent such as water or ethanol before use. The fruit juice can be used, for example, as a juice obtained by squeezing a fruit (raw fruit juice), a diluted raw fruit juice, a concentrated fruit juice, and a concentrated reduced fruit juice.
Concentrated fruit juice refers to raw fruit juice from which the water content in the fruit juice has been removed by a heat concentration method or a frozen concentration method to increase the concentration of the fruit juice. A fruit juice diluted with a solvent such as water so that it has the same concentration as the original fruit juice.

The juice of Yukou may be obtained by squeezing a fruit or by squeezing a juice containing a pericarp. Examples of the squeezing method include, but are not limited to, a method of squeezing by hand and a method of squeezing by a device generally used in the art.

The "Yuko extract" in the present invention is obtained by extracting Yukou with various solvents as a raw material. Examples of the Yukou extract include a pericarp extract, a fruit juice squeezed residue extract, and the like.
Examples of the extraction method include, but are not limited to, a hot water extraction method, a boiling extraction method, a dipping extraction method, a shaking extraction method, a Soxhlet extraction method, and a steam distillation method.

As the extraction solvent, for example, water, an organic solvent or a mixed solvent thereof can be used. Examples of the organic solvent include lower alcohols such as ethanol, chloroform, ethyl acetate, and n-hexane. Water and ethanol are preferred. Further, two or more kinds of these solvents may be mixed and used.

The amount of the extraction solvent used can be appropriately determined depending on the site of the yukou, the type of the extraction solvent, and the like. The extraction time is, for example, 1 minute to 50 days, preferably 1 hour to 30 days, and the extraction temperature is, for example, 0 ° C to 100 ° C, preferably 10 ° C to 80 ° C.

The Yukou extract can be used as it is in the extracted solution, and further purification treatment can be added if necessary. The purification treatment may be carried out by a usual method, and can be purified by, for example, filtration, chromatography or the like. Then, the obtained filtrate can be concentrated under reduced pressure, freeze-dried, or the like to obtain a dried product of Yukou.

The "antibacterial composition" in the present invention refers to a composition having effects such as bacteriostatic (suppression of bacterial growth), sterilization, sterilization, disinfection, sterilization, antiseptic, and antifungal. Targets include, but are not limited to, bacteria and fungi.

Examples of bacteria include Gram-negative bacteria such as Escherichia coli, Pseudomonas aeruginosa, Salmonella, Moraxella, and Regionella; and Gram-positive bacteria such as Staphylococcus aureus and Clostridium. Examples of fungi include yeasts such as Candida, Rhodotorula, baker's yeast; molds such as red mold and black mold.

The "composition for intestinal regulation" in the present invention means a composition for improving the function of the intestine to a normal state. For example, it refers to a composition that improves the composition of the intestinal flora (a state in which bad bacteria are increasing) deteriorated by ingesting a high-fat diet to the composition of a normal intestinal flora.

The "deterioration of the intestinal flora" in the present invention refers to a state in which lactic acid bacteria and bifidobacteria belonging to good bacteria are decreasing, and Clostridium perfringens and staphylococci belonging to bad bacteria are increasing. It has been reported that lifestyle disorders such as excessive energy intake, increased lipid intake, and lack of exercise, especially the intestinal environment changes depending on the dietary content, and a diet centered on animal foods (animal protein and saturated fat). Is known to increase bad bacteria and decrease good bacteria as compared with vegetable foods.

The "composition for preventing and / or ameliorating periodontal disease and / or bad breath" in the present invention refers to oral bacteria (periodontal disease bacteria) that cause periodontal disease, for example, Streptococcus, Veillonella, Prevotella, Actinomyces. A composition that suppresses the increase of the genus, Rothia, and Granulicatella, and reduces the concentration of hydrogen sulfide and methyl mercaptan that cause bad breath. Also included are compositions that increase antimicrobial peptides (eg, human β-defensin 1) in the oral cavity.

The "composition for preventing and / or ameliorating diabetes" in the present invention refers to a composition for preventing, ameliorating and / or treating a disease causing diabetes and the subjective symptoms associated therewith. For example, it refers to a composition that improves glucose intolerance caused by continuous intake of a high-fat diet.

The "high-fat diet-induced glucose intolerance abnormality" in the present invention refers to insulin resistance caused by continuing a high-fat diet. As insulin resistance progresses, it becomes type 2 diabetes.

"Improvement" in the present invention means improvement of symptoms / conditions of a disease, prevention / delay of deterioration of symptoms / conditions of a disease, reversal of progression of symptoms of a disease, prevention / delay or treatment of a disease, and "prevention". "" Means to prevent or delay the onset of the disease in the subject or reduce the risk of developing the disease in the subject. "Treatment" in the present invention means any treatment of a disease (eg, improvement, alleviation, suppression of progression, etc.). It may also include blocking the onset and / or progression of the disease.

The "patient" in the present invention means humans and other animals such as dogs, cats, horses and the like. The patient is preferably a mammal, more preferably a human.

As used in the present invention, "therapeutically effective amount" means the amount of an active ingredient in a tissue, system, animal or human that induces a biological or pharmaceutical response required by a researcher or physician. Also, "therapeutically effective amount" includes the meaning of the amount of active ingredient sufficient to bring about such treatment of the disease when administered to a mammal to treat the disease. do. The "therapeutically effective amount" varies depending on the active ingredient, the disease and its severity, and the age, weight, etc. of the mammal to be treated.

Further, the "effective amount" includes the meaning of the amount of the active ingredient that produces an acute or chronic therapeutic effect when administered at an appropriate dose. This therapeutic effect includes to a detectable degree of prevention, correction, inhibition, or reversal of the symptoms, signs, and underlying pathology of the disease / condition and associated complications.

The "food composition" in the present invention is anything other than a pharmaceutical composition and may be in a form that can be orally ingested, such as a solution, suspension, emulsion, powder, or solid molded product, and is particularly limited. It is not something that is done.

Examples of the food composition include instant foods such as instant noodles, retort foods, canned foods, instant soups / miso juices, and freeze-dried foods; beverages such as soft drinks, vegetable drinks, powdered drinks, concentrated drinks, and alcoholic drinks; Wheat flour products such as bread, noodles and bread flour; sweets such as candy, caramel, chewing gum, chocolate, cookies, biscuits, cakes, crackers, Japanese sweets and dessert sweets; seasonings such as sauces, dressings and soups; butter, margarine , Oils and fats such as mayonnaise; dairy products such as dairy beverages, yogurts, ice creams and creams; processed agricultural products such as jams and marmalades and cereals; frozen foods, etc., but are limited to these. is not.

The antibacterial composition of the present invention may be blended and used in personal care products and home care products. That is, it is also possible to provide personal care products and home care products containing the antibacterial composition of the present invention.

Personal care products containing the antibacterial composition of the present invention include, for example, soaps, hand soaps, body cleansers, oral cleansers, toothpastes, shampoos, conditioners, hair conditioners, antiperspirants, body lotions, deodorants and the like. However, it is not limited to these.

Household care products containing the antibacterial composition of the present invention include, for example, laundry detergents, softeners, deodorants, fabric cleaning compositions, dishwashing detergents, automatic dishwashing detergents, floor care compositions, kitchen cleaners. , Kitchen disinfectants, bathroom cleaners, bathroom disinfectants, etc., but are not limited to these.

Further, since the yukou used in the antibacterial composition of the present invention is a natural material, it may be blended with foods and drinks or cosmetics and used as a preservative.

The method of using the antibacterial composition of the present invention as a preservative may be added to foods and drinks and cosmetics by an appropriate method, and is not particularly limited.

Examples of foods and drinks containing the antibacterial composition of the present invention include, but are not limited to, sprinkles added to rice and toppings and jams added to breads. Examples of cosmetics containing the antibacterial composition of the present invention include, but are limited to, basic cosmetics such as creams, milky lotions, lotions, facial cleansers, and facial masks, and make-up cosmetics such as lipsticks and foundations. It's not a thing.

The blending amount of the antibacterial composition of the present invention into foods and drinks or cosmetics varies depending on the type of foods and drinks or cosmetics, and an amount suitable for the purpose may be added.

The composition of the present invention may contain Yukou or an extract thereof as an active ingredient, and may further contain an excipient or the like as long as the effect of Yukou or an extract thereof is not impaired. Therefore, the ratio of Yukou or its extract in the composition of the present invention is not particularly limited. Alternatively, the composition of the present invention may consist only of Yukou or an extract thereof.

The compositions of the present invention can be administered orally or parenterally (eg, transgastric, transintestinal). The composition of the present invention can be used as a pharmaceutical or food composition.

In the present invention, when the composition of the present invention is prepared as a pharmaceutical composition (pharmaceutical product, quasi-drug, etc.), Yukou or an extract thereof, other medicinal ingredients, a pharmaceutically acceptable carrier and an addition thereof. The agent can be blended. For example, as a pharmaceutically acceptable carrier and additive, a binder, a disintegrant, a lubricant, a wetting agent, a buffering agent, a fragrance and the like can be mixed to prepare a desired dosage form. The dosage form of the drug is not particularly limited, but is an injection, an external preparation, an inhaler, a suppository, a film, a troche, a liquid, a powder, a tablet, a granule, a capsule, a syrup, or an eye drop. Examples include agents, eye wash agents, and nasal drops.

In the present invention, the composition of the present invention is prepared as a food composition (health food, functional food, dietary supplement, supplement, health functional food, specified health food, nutritional functional food, functionally labeled food, etc.). If you use Yukou or its extract, sweeteners, colorants, thickeners, stabilizers, gelling agents, pastes, antioxidants, color formers, bleachers, yeast foods, gum bases, fragrances, acidulants, Seasonings, emulsifiers, pH adjusters, pans, swelling agents, nutritional enhancers or other food and beverage materials can be mixed to prepare the desired form.

Hereinafter, the present invention will be described in more detail with reference to examples, but the present invention is not limited thereto.

Example 1: Examination of the effect of suppressing the deterioration of the intestinal bacterial flora by a high-fat diet with yukou juice (1) Method 6-week-old male C57B16 mice (Japan SLC Co., Ltd .; n = 20) were subjected to 2 in an SPF environment. Raised for a week. Eight-week-old C57B16 mice were randomly administered in 4 groups (normal diet group (100% saline juice administration), normal diet group (physiological saline administration), high-fat diet (HFD-60%) group (100% yukou juice administration). ), The high-fat diet group (physiological saline administration)) (n = 5 per experimental group), and 200 μL of 100% Yukou juice or physiological saline was orally administered daily for 7 days to the mice in each group. After 7 days, feces were collected, DNA was extracted, and intestinal flora analysis was performed by the T-RFLP method at ICLAS (Central Institute for Experimental Animals) to analyze the intestinal flora.

(2) Results The results are shown in FIG. FIG. 1 shows the composition of the intestinal flora in each group.
In the high-fat diet group, lactic acid bacteria and bifidobacteria are reduced, Clostridium bacteria are increased, and the composition of the intestinal flora is deteriorated as compared with the normal diet group. In the normal diet group and the high-fat diet group, administration of Yukou juice improved the deterioration of the composition of the intestinal flora. Therefore, it was proved that Yukou juice has an effect of improving intestinal function.
In addition, in the Yukou juice-administered group, the appearance of Akkermansia bacterium, which is said to have an anti-diabetic effect in both the normal diet group and the high-fat diet group, was observed.

Example 2: Examination of antibacterial effect of Yukou fruit juice For Escherichia coli strain K12, Staphylococcus aureus and bread yeast (fungus), growth inhibition circle formation on ordinary agar medium was observed, and the antibacterial activity of Yukou fruit juice was evaluated.
(1) Method
(1-i) Soak circular filter paper with Yukou juice (30%, 60% or 100%) to make Yukou juice-containing discs, place each disc on a plate seeded with Escherichia coli strain K12, and place each disc at 37 ° C. 24. After culturing for hours, the formation of blocking circles was observed.
(1-ii) Place each disc impregnated with 100% fruit juice of citrus, sudachi or yuzu on a plate inoculated with Staphylococcus aureus, incubate at 37 ° C for 24 hours, observe the formed blocking circle, and citrus. And other citrus fruits were compared in antibacterial activity. In addition, each disc impregnated with 100% fruit juice of Yuzu or Yuzu was placed on a plate seeded with baker's yeast (fungus) and cultured at 37 ° C. for 24 hours, and the formation of blocking circles was observed.

(2) Results The results are shown in FIG. FIG. 2 shows (A) a circle of inhibition for Escherichia coli strain K12, (B) a circle of inhibition for Staphylococcus aureus, and (C) a circle of inhibition for baker's yeast (fungus).
Yukou juice was observed to have a clear inhibition circle against Escherichia coli strain K12, Staphylococcus aureus and fungi. On the other hand, Sudachi juice and Yuzu juice did not show a blocking circle against Staphylococcus aureus and fungi. That is, it is recognized that Sudachi juice and Yuzu juice cannot sufficiently suppress the growth of bacteria and fungi.

From the above results, it was proved that Yukou juice has an excellent antibacterial effect and further has an excellent antibacterial effect as compared with other citrus fruits.

Example 3: Comparative study of antibacterial effect between Yukou juice and existing preservatives For Escherichia coli (E. coli) and resistant thermoacidic bacteria (AAT; Alicyclobacillus acidterestris), Yukou juice (1%, 5%) , 25%, 50%, 75% and 100%), benzoic acid (60ppm, 125ppm, 190ppm and 250ppm) and acetic acid (0.15%, 0.23% and 0.30%) were used to compare antibacterial activity.
(1) Method 100% of Yukou juice was diluted with distilled water to prepare diluted solutions of 1%, 5%, 25%, 50% and 75% of Yukou juice. Benzoic acid and acetic acid were used as controls. Various concentrations of Yukou juice, benzoic acid and acetic acid were cultured with 103 Escherichia coli or AAT at ³⁷ ° C for 16 hours, and the number of colonies formed on the plate was counted.

(2) Results The results are shown in FIG. FIG. 3 shows (A) the number of colonies formed by Yukou juice, benzoic acid and acetic acid against Escherichia coli, and (B) the number of colonies formed by Yukou juice, benzoic acid and acetic acid against AAT bacteria.
50% diluted solution of Yukou juice, 75% diluted solution and 100% of Yukou juice, like benzoic acid and acetic acid used as preservatives for food and drink, show almost no colony formation and kill Escherichia coli and AAT bacteria. It is recognized that there is. In other words, when the concentration of Yukou juice was used at 50% to 100%, it exerted an antibacterial effect, suggesting that Yukou juice with a concentration of 50% to 100% can be used as a preservative for food and drink.

Example 4: Examination of the effect of improving the oral environment by Yukou juice <br /> Method: A healthy person is allowed to continuously ingest 50 cc of 100% Yukou juice for one week, and oral volatile sulfides (hydrogen sulfide and methyl) before and after ingestion. The concentration of mercaptan) was measured with an oral chroma (manufactured by Nippon Dental Trading Co., Ltd.), which is a halitosis measuring device (VSC monitor). Similar measurements were made 7 times.
Furthermore, saliva was collected from a healthy person, DNA was extracted from the collected saliva sample, and the number of oral bacteria (number of periodontal disease bacteria) was analyzed by the T-RFLP method at Biological Giken Co., Ltd.
In addition, the amount of human β-defensin 1 which is an antibacterial peptide was measured by a human beta defensin 1 ELISA kit (manufactured by Signal Way Antibody).

上記の結果から、ユコウ果汁を摂取することにより、口臭の原因である硫化水素およびメチルメルカプタンの濃度が有意に低下した。そのため、ユコウ果汁には口臭抑制効果があると認められる。 (2) Results The average values of the concentrations of oral volatile sulfides (hydrogen sulfide and methyl mercaptan) before and after ingestion of Yukou fruit juice are shown in Table 1 below.

From the above results, ingestion of Yukou juice significantly reduced the concentrations of hydrogen sulfide and methyl mercaptan, which are the causes of bad breath. Therefore, it is recognized that Yukou juice has an effect of suppressing bad breath.

The results regarding the improvement effect of periodontal disease are shown in FIG. FIG. 4 shows (A) the number of oral bacteria (number of periodontal disease bacteria) before and after ingestion of Yukou fruit juice, and (B) human β, which is an antibacterial peptide before and after ingestion of Yukou fruit juice. -The amount of defensin 1 (pg / ml) is shown.
Continued ingestion of Yukou juice for one week significantly reduced the periodontal bacteria Streptococcus, Veillonella, Prevotella, Actinomyces, Rothia and Granulicatella, and the antibacterial peptide human β-defensin 1. The amount of was significantly increased. That is, it was proved that Yukou juice has an effect of improving periodontal disease.

From the above results, it is recognized that Yukou juice can improve the oral environment by improving bad breath and periodontal disease.

Example 5: Examination of bacteriostatic effect of Yukou soap (1) Method Dissolve a natural ingredient solid soap containing 3% of Yukou juice provided by Clean Maker Co., Ltd. in distilled water and dissolve Yukou soap solution (0.1 g). / 100 μL) was prepared. The prepared Yukou soap solution and 103 E. coli ^were mixed and cultured with shaking at 37 ° C for 1 hour, and the number of E. coli was measured.

(2) Results The results are shown in FIG. FIG. 5 shows the ratio of the number of bacteria after addition of Yukou soap solution (0.1 g / 100 μL) when the normal number of bacteria is 100%.
It was confirmed that the number of bacteria was reduced by about 20% by adding the Yukou soap solution.

Example 6: Examination of improvement effect of high-fat diet-induced glucose intolerance by yukou juice (1) Method A 6-week-old C57B16 mouse (purchased from Japan SLC) was given a high-fat diet (HFD-60) for 28 days. At the same time, 200 μL of 100% Yukou juice was orally administered once a day for 28 days (n = 6). In the control group, saline was administered instead of 100% Yukou juice (n = 6). After 28 days, the patient was fasted overnight and an oral glucose tolerance test was performed.

(2) Results The results are shown in FIG. FIG. 6 shows the blood glucose levels in the Yukou-administered group and the physiological saline-administered group (control group) after 0 to 120 minutes of glucose loading.
In the oral glucose tolerance test, the blood glucose level 30 minutes after glucose loading was significantly lower in the Yukou-administered group than in the control group. This result indicates that Yukou juice has an effect of improving high-fat diet-induced glucose intolerance.
There was no significant difference in body weight between the Yukou juice-administered group and the physiological saline-administered group for 4 weeks.

Since the present invention uses Yukou or an extract thereof as an active ingredient, it is useful not only as a pharmaceutical composition but also as a food composition such as foods and drinks and supplements, cosmetics, and care products, and in a wide range of fields. It is possible to provide a highly safe composition.

Claims (2)

An antibacterial composition against Staphylococcus aureus or baker's yeast (fungus) containing Staphylococcus aureus or an extract thereof as an active ingredient.
An antibacterial composition, wherein the yukou or an extract thereof is a fruit juice of yukou . The antibacterial composition according to claim 1, wherein the fruit juice is a juice obtained by squeezing a fruit (raw fruit juice), a diluted raw fruit juice, a concentrated fruit juice, or a concentrated reduced fruit juice . ..

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