JP7106345B2 - Periodontal disease inhibitor and oral composition using the same - Google Patents

Description

TECHNICAL FIELD The present invention relates to novel periodontal bacteria inhibitors and oral compositions.

An extremely large variety of bacteria exceeding 500 to 700 species have been found in the human oral cavity, and they constantly form aggregates, ie, bacterial flora, on the mucosal surface. The balance of this oral flora is maintained by complex interactions, and by antagonizing foreign bacteria, it prevents their invading infection, that is, it also has a function of conferring resistance.

On the other hand, however, periodontal disease-causing bacteria also exist in the oral cavity, and the periodontal disease caused by these bacteria is said to be the intraoral infectious disease that causes the largest number of infected people.

Periodontal diseases caused by periodontal bacteria include gingivitis, periodontitis and occlusal trauma. In addition, chronic periodontal disease is known to cause infectious diseases such as respiratory disease, heart disease, diabetes, premature birth, etc., caused by periodontal disease bacteria.

The main cause of periodontal disease is bacteria in dental plaque that accumulates in periodontal pockets. Gram-positive bacteria usually occupy most of the periodontal pockets of healthy individuals, but as periodontal disease progresses, Porphyromonas gingivalis, Fusobacterium nucleatum, etc. Gram-negative bacteria called periodontal disease bacteria are known to increase.

Numerous studies have been conducted so far on the suppression of periodontal disease bacteria, and toothpastes, mouthwashes and the like containing antibacterial substances effective against these bacteria have been developed. For example, as an antibacterial agent against periodontal disease bacteria, cocoa contained in cacao mass is used as an active ingredient (Patent Document 1), Scutellaria baicalensis extract or the like is used as an anti-caries agent (Patent Document 2), and allyl isothiocyanate. An anti-periodontal disease agent containing an isothiocyanate compound such as a mint essential oil or its essential oil component (Patent Document 3), an anti-periodontal disease agent containing cinnamon bark extract as an active ingredient (Patent Document 5), thyme extract A composition for preventing periodontal disease (Patent Document 6) containing a substance, essential oil and one or more of the oleoresin group is known.

However, few products developed so far are generally satisfactory in terms of efficacy, safety, feeling of use, etc. As the population ages, prevention of oral diseases has become an important issue. Therefore, development of a more excellent inhibitor of periodontal disease bacteria is required.

On the other hand, there are reports that sesquiterpenes contained in coniferous trees have an antibacterial effect against Streptococcus mutans and suppress biofilm formation (Patent Document 4 and Non-Patent Document 1). It has also been reported that 1,8-cineole is effective against Streptococcus mutans (Non-Patent Document 2). However, these are not about periodontal disease bacteria, and do not suggest application to periodontal disease bacteria.

WO 2003/99304 pamphlet JP-A-2001-97835 Japanese Patent Application Laid-Open No. 2007-308378 JP 2012-229191 A JP-A-4-29933 JP-A-3-255031

2017 44th Annual Meeting of Japan Anti-Mold Society P185 Abstracts of the 57th Annual Meeting of the Japanese Society of Chemotherapy P164

Accordingly, an object of the present invention is to provide a highly practical agent for suppressing periodontal disease bacteria in terms of efficacy, safety, feeling of use, etc., and an oral composition using the same.

In order to solve the above problems, the present inventors have been searching for antibacterial components in natural products, and found that there is a substance that has the effect of significantly suppressing periodontal disease bacteria in the branches and leaves of specific conifers. The discovery led to the completion of the present invention.

That is, the present invention is a periodontal bacteria inhibitor characterized by containing, as an active ingredient, a pressed liquid, extract or distillate of a plant belonging to the genus Fir of the family Pinaceae.

The present invention also provides an oral composition comprising, as active ingredients, a pressed liquid, extract or distillate of a plant belonging to the genus Fir of the family Pinaceae, and an oral composition base.

Furthermore, the present invention is a method for suppressing periodontal disease bacteria, which comprises applying the above periodontal disease inhibitor or oral composition to the oral cavity.

The agent for suppressing periodontal disease of the present invention can remarkably suppress the growth of periodontal disease bacteria in the oral cavity, and therefore is effective for the treatment and prevention of periodontal disease.

In addition, the oral composition of the present invention, which is in the form of a dosage form for daily use, contains a component that inhibits periodontal disease bacteria, so that it is possible to easily prevent periodontal disease without being particularly conscious of it. It is.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a diagram schematically showing the configuration of a microwave distillation apparatus used to obtain a distillate of a plant belonging to the genus Fir of the family Pinaceae, which is used as an active ingredient of the periodontal bacteria inhibitor of the present invention.

The active ingredient of the agent for inhibiting periodontal disease bacteria of the present invention is a pressurized liquid, extract or distillate of a plant belonging to the genus Fir of the family Pinaceae (hereinafter sometimes referred to as "periodontal disease inhibitory ingredient").

Plants belonging to the genus Fir of the Pinaceae family, which are raw materials for this periodontal disease-suppressing ingredient, include Sakhalin fir, fir, fir fir, white fir, white fir, white fir, white fir, balsam fir, honeysuckle fir, white fir, amabilis fir, Japanese fir, California red fir, and ground. Fur, noble fur and the like can be mentioned. Among these, Sakhalin fir is preferably used because of its excellent effect on periodontal disease bacteria.

For the preparation of the periodontal disease-suppressing component of the present invention, the whole plant body or a part thereof (for example, fruit, seed, leaf, bark, rhizome, flower, etc.) of the plant belonging to the genus Fir of the Pinaceae family is used. Among these, the leaf portion is preferably used from the viewpoint of effective utilization of trees to be thinned and pruned and from the viewpoint of a high suppressing effect. Although the leaf portion may be used as it is, it is preferably used after being pulverized and crushed by a pulverizer, a crusher, or the like.

The periodontal disease-inhibiting component is obtained as a juice, extract, or distillate from the whole or part of a plant belonging to the genus Fir of the family Pinaceae. Among these, the squeezed liquid can be produced using a known press. The extract can also be produced according to a conventional method, but it is preferable to use a polar solvent as the extraction solvent. Specifically, water, ethanol, methanol, butyl alcohol, ethylene glycol, propylene glycol, glycerin. halogen-containing solvents such as chloroform; ether solvents such as dioxane; carbonyl solvents such as acetone and ethyl acetate; and mixtures thereof. A distillate can be obtained by atmospheric distillation, vacuum distillation, steam distillation, or the like.

Among these, the active ingredient of the agent for suppressing periodontal disease bacteria of the present invention is preferably a distillate, and particularly preferably one obtained by distillation by heating under reduced pressure. This product contains monoterpenes at a high concentration and has a refreshing aroma without the need for concentration, purification, etc., so that the aroma is highly palatable and has a high inhibitory effect on periodontal disease bacteria. Therefore, it is preferable.

Heating for the distillation may be performed by heating with a general heater or the like, but heating with microwaves is preferable from the viewpoint of heating efficiency, and it is particularly preferable to heat by irradiating microwaves without adding water. By irradiating microwaves, the water molecules contained in the plant are directly heated to generate water vapor, which acts as a mobile phase to distill the volatile components in the plant. It is believed to include vacuum distillation elements and steam distillation elements depending on the boiling points of the components.

Such a microwave-irradiated distillate can be obtained, for example, by using the apparatus shown in FIG. In the figure, 1 is a microwave distillation device, 2 is a distillation tank, 3 is a microwave heating device, 4 is a stirring splash, 5 is an air flow inlet pipe, 6 is a distillate outlet pipe, 7 is a cooling device, and 8 is a heating control. An apparatus, 9 is a decompression pump, 10 is a pressure regulating valve, 11 is a pressure control device, 12 is an object to be distilled, and 13 is a distillate.

In this apparatus 1, a plant belonging to the genus Fir of the Pinaceae family, which is a raw material to be distilled 12, is placed in a distillation tank 2, and stirred with a stirring blade 4. A microwave heating device is provided on the upper surface of the distillation tank 2. Microwaves are emitted from 3 to heat the raw material. The distillation tank 2 communicates with an air flow inlet 5 and a distillate outlet pipe 6 . The airflow inlet pipe 5 introduces air or an inert gas such as nitrogen gas into the distillation tank 2 , and this airflow is introduced from the bottom of the distillation tank 2 . Further, the distillate outflow pipe 6 discharges the distillate from the raw material to the outside from the upper part of the distillation tank 2 .

Inside the distillation tank 2, the temperature and pressure are measured by a temperature sensor and a pressure sensor (both not shown) attached thereto. 10 to be adjusted respectively.

Further, the gaseous distillate discharged from the distillation tank 2 through the distillate outlet pipe 6 is converted into a liquid by the cooling device 7 and obtained as the distillate 13 . The distillate 13 contains an oily fraction 13a and an aqueous fraction 13b, of which the oily fraction 13a is preferably used.

Distillation may be carried out at a pressure of 3 to 95 kilopascals, preferably 3 to 40 kilopascals, more preferably 3 to 20 kilopascals, and the steam temperature is 40°C. to 100°C. When the pressure is 3 kpa or less, the increase in vapor pressure of volatile components in the plant is suppressed, and the boiling point of each component becomes more important than the steam distillation factor, and the components with the lowest boiling point flow out in order. Therefore, it is not preferable in that the extraction of components having a boiling point higher than that of water is not efficiently performed, and that a large amount of high boiling point components with low repelling efficiency and poor odor preference are extracted. On the other hand, if the temperature is 95 kilopascals or more, the temperature of the raw material becomes high, which is not preferable in that the energy loss is large and the oxidation of the raw material is accelerated.

Also, the distillation time should be about 0.2 to 8 hours, preferably about 0.4 to 6 hours. If it is less than 0.2 hours, many unextracted components in the plant will remain, and if it is more than 8 hours, the raw material will be in a state close to dryness, which is not preferable in that the extraction efficiency is lowered.

Furthermore, the gas introduced into the distillation tank 2 may be air, but inert gases such as nitrogen gas, helium gas, and argon gas are preferable. 0.001 to 0.1 times the capacity.

As the component for inhibiting periodontal disease bacteria of the present invention, the juice, extract or distillate of the plant belonging to the genus Fir of the family Pinaceae can be used as it is. , may be used after further concentration or purification.

In the periodontal disease inhibitor of the present invention, the above periodontal disease bacteria inhibiting component may be used as it is, or may be used in combination with a suitable solvent.

Examples of the solvent used in the periodontal disease inhibitor of the present invention include water, alcohol solvents such as ethanol, isopropyl alcohol and propyl alcohol, glycol solvents such as propylene glycol and ethylene glycol, ethylene glycol monomethyl ether, and propylene. Glycol ether solvents such as glycol monomethyl ether, propylene alcohol monoethyl ether, and 3-methoxy-3-methyl-1-butanol can be used.

When the periodontal disease inhibitor is prepared using a solvent, the amount of the periodontal disease inhibitor is preferably 0.01% by mass (hereinafter simply referred to as "%") to about 99%. is between 0.1% and 20%.

The amount of the periodontal disease-suppressing component in the periodontal disease-suppressing agent of the present invention is not particularly limited as long as the effect can be confirmed. is set in the formulation in the range of 0.001 mg/g to 200 mg/g, preferably in the range of 0.05 mg/g to 20 mg/g.

The agent for inhibiting periodontal disease bacteria of the present invention may be blended with other conventionally known ingredients for inhibiting periodontal disease bacteria, thereby exhibiting a more excellent effect of inhibiting periodontal disease bacteria. Examples of these other periodontal bacteria inhibiting ingredients include cetylpyridinium chloride, isopropylmethylphenol, lauroyl sarcosine salt, chlorhexidine, tranexamic acid, tetracycline, minocycline, lemongrass mastic oil, chamomile tincture, ratania tincture, hinokitiol, sandalwood. , cassia, cinnamon, guaiac, patchouli essential oil, gallic acid, catechins, red reishi, black reishi, yellow lily leaf, green tea, mugwort, Chinese quince, prickly pear, gymnema, rooibos tea, hawthorn, turmeric, licorice, silymarin, cinnamon , purple brown rice, eleuthero cock, shell ginger leaf, dokudami, large jujube, reishi mushroom, garlic, chimp, licorice, chamomile, chazenwort, bellflower, cinnamon, fennel, hops, valerian, scutellaria root, Chinese radish, sage, ginkgo, bamboo, sorghum , reishi, red ginger, rice extract, etc., and these can be used singly or in combination of two or more.

The agent for suppressing periodontal disease bacteria of the present invention can also be used as an oral composition in combination with a known oral composition base. The composition for oral cavity may be in any form such as liquid, solid, powder, paste, etc., and it is possible to arbitrarily select the optimum dosage form for carrying out the present invention.

Specific oral compositions include dentifrices such as powdered toothpaste, toothpaste, wet toothpaste, and liquid toothpaste, mouthwashes, mouth fresheners, gargling tablets, denture cleansers, gargles, lozenges, and chewing gums. etc. various dosage forms can be mentioned.

The oral composition base used in the preparation of the oral composition of the present invention includes known abrasives, surfactants, and thickeners as long as they are of a type and within a range that does not impair the effects of the present invention. , sweeteners and other components and additives, and these can be selected and used in combination.

Among these, as abrasives, for example, silica-based abrasives such as anhydrous silicic acid, aluminum hydroxide, aluminum oxide, aluminosilicate, calcium hydrogen phosphate, dicalcium phosphate, insoluble sodium metaphosphate, insoluble potassium metaphosphate, titanium oxide , synthetic resins, etc. are preferably used as necessary. These abrasives can be blended singly or in combination of two or more.

Examples of surfactants include water-soluble salts of higher alkyl sulfate esters in which the alkyl group has 8 to 18 carbon atoms, such as sodium lauryl sulfate and sodium myristyl sulfate, sodium lauryl monoglyceride sulfonate, sodium coconut monoglyceride sulfonate, and the like. Water-soluble salts of higher fatty acid monoglyceride sulfonic acid, olefin sulfonic acid, paraffin sulfonic acid, other anionic active agents, stearyl monoglyceride, sucrose mono and dilaurate, etc. Sucrose fatty acid ester having 12 to 18 carbon atoms, lactose fatty acid ester, lactitol fatty acid ester, maltitol fatty acid ester, stearic acid monoglyceride, polyoxyethylene sorbitan monolaurate, polyoxyethylene hydrogenated castor oil, ethylene glycol about 60 Interfaces such as nonionic active agents such as sorbitan monostearate condensates with molar additions, polymers of ethylene oxide and propylene oxide, and derivatives such as polyoxyethylene polyoxypropylene monolauryl ester, amphoteric active agents such as betaine type and amino acid type. Active agents may be mentioned, and one or two or more of these may be mixed and blended.

Furthermore, thickening agents include, for example, carrageenan, cellulose derivatives such as sodium carboxymethylcellulose, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, sodium carboxymethylhydroxyethylcellulose, alkali metal alginates such as sodium alginate, propylene glycol alginate, xanthan gum, tragacanth gum, Gums such as karaya gum and gum arabic, synthetic thickeners such as polyvinyl alcohol, sodium polyacrylate, carboxyvinyl polymer and polyvinylpyrrolidone, inorganic thickeners such as gelling silica, gelling aluminum silica, veegum and laponite etc., and one or more of these can be blended.

Furthermore, as a thickening agent, one or more of sorbit, glycerin, ethylene glycol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, polypropylene glycol, xylit, maltit, lactit and the like can be blended.

Furthermore, sweeteners such as sodium saccharin, stepioside, xylitol, neohesperidyl dihydrochalcone, glycyrrhizin, perillartine, thaumatin, aspartylphenylalanine methyl ester, p-methoxycinnamic aldehyde, sodium cyclamate, etc., and preservatives. , for example, p-hydroxymethylbenzoic acid, p-hydroxyethylbenzoic acid, p-hydroxypropylbenzoic acid, p-hydroxybutylbenzoic acid, sodium benzoate, lower fatty acid monoglyceride, etc., as fragrances, for example, winter Green oil, spearmint oil, peppermint oil, sassafras oil, clove oil, and eucalyptus oil can be mentioned, respectively, and these can be blended as needed.

In addition, other ingredients such as gelatin, peptone, albumin, brighteners, silicones, dyes, fluorides such as sodium fluoride, sodium monofluorophosphate, tin fluoride, epsilon aminocaproic acid, dextranase, Enzymes such as protease, mutanase, lysozyme, lytic enzyme, lytech enzyme, chlorhexidine salts, dihydrocholesterol, glycyrrhetin salts, glycyrrhetinic acid, caropeptides, vitamins, antibacterial agents, and other medicinal ingredients such as desensitizing agents for hypersensitivity such as potassium nitrate. One or more kinds can also be blended.

The pH of the composition for oral use of the present invention is not particularly limited as long as it is within a range that poses no problem in terms of safety in the oral cavity and the human body. be. In addition, if necessary, as a pH adjuster, acetic acid, hydrochloric acid, sulfuric acid, nitric acid, citric acid, phosphoric acid, sodium hydroxide, potassium hydroxide, sodium acetate, sodium carbonate, sodium citrate, sodium hydrogen citrate, phosphorus An appropriate amount of sodium acid, sodium hydrogen phosphate, or the like can be added.

A preferred embodiment of the oral composition of the present invention is a toothpaste formulation containing calcium hydrogen phosphate, glycerin and water as the oral composition base.

Another preferred embodiment includes a mouthwash dosage form containing ethanol and water as oral composition bases.

Further, another preferred embodiment includes an oral moisturizer dosage form containing glycerin and water as oral composition bases.

For the periodontal disease inhibitor described above, for example, a liquid preparation containing an effective amount of the periodontal disease inhibitor may be put in the mouth about once to three times a day, followed by rinsing and gargling. As for the oral composition, a composition containing an effective amount of a periodontal disease-suppressing component may be used in a usual manner corresponding to the dosage form.

The present invention will be described in more detail below with reference to Production Examples, Examples and Formulation Examples, but the present invention is not limited to these.

Manufacturing example 1
Sakhalin fir leaves were used as a raw material, and Sakhalin fir essential oil was obtained in the following manner. That is, about 50 kg of Sakhalin fir leaves pulverized by a crushing type pulverizer (manufactured by KYB Seisakusho) is put into a distillation tank of a microwave distillation apparatus as shown in FIG. It was held under reduced pressure conditions of 20 KPa (vapor temperature was about 67° C.) and irradiated with microwaves for 1 hour to distill the essential oil. The amount of essential oil obtained was 180 mL and the ratio of essential oil to input sample was 0.34%.

Manufacturing example 2
Using the same Sakhalin fir leaves as in Production Example 1, Sakhalin fir essential oil was obtained by the following steam distillation method. That is, about 101 g of Abies sachalinensis leaves pulverized by a compression type pulverizer (manufactured by KYB Manufacturing) are placed in a Pyrex (registered trademark) glass flask, 5 to 8 times the amount of water is added, and then the flask is placed in a hot water bath at 90°C. Heat to ~100°C and bring to a boil. The essential oil collection tube was filled with water to the baseline prior to heating. Boiling was continued for 6 hours to distill the essential oil, yielding 0.8 mL of the essential oil. The percentage of essential oil to input sample was 0.79%.

Example 1
Preparation of periodontal bacteria inhibitor:
Each component was taken at the ratio shown in the formulation in Table 1 below and mixed to prepare the periodontal disease inhibitor of the present invention. In addition, as comparative products 1 to 3, eucalyptus oil, tea tree oil, or tea extract, which is generally said to be effective against periodontal disease bacteria, was added instead of Sakhalin fir essential oil. , a surfactant dissolved in a water-ethanol mixture (control product 1) and a water-ethanol mixture alone (control product 2) were prepared.

<Prescription>

Example 2
Periodontal bacteria inhibitory effect test:
Using 10 g of each sample prepared with the composition of Example 1, the periodontal disease inhibitor of the present invention (the product of the present invention), each comparative product, and oral microorganisms containing periodontal bacteria of the control product were tested by the following test method. was investigated for its antibacterial effect on

<Test method>
A specified medium corresponding to the microorganism to be tested was placed in each well of a 96-well plate, and solutions of the present invention product, each comparative product and each control product diluted to 31.25 to 4000 ppm were added thereto.

Then, each precultured bacterium shown in Table 2 below was inoculated into each well, and cultured for 48 hours at culture temperature and culture conditions corresponding to the microorganism to be tested. After completion of culturing, the presence or absence of bacterial growth was visually confirmed, and the minimum concentration at which no growth was observed was defined as the minimum inhibitory concentration (MIC). The results are shown in Table 3.

From the above results, it was confirmed that the product of the present invention has a remarkable inhibitory effect on two types of periodontal disease bacteria (P.gingivalis and F.nucleatum). This was a stronger antibacterial activity than eucalyptus oil, tea tree oil, and tea extract, which are conventionally believed to be effective against periodontal disease bacteria. On the other hand, the product of the present invention showed no effect against fungi such as caries-causing bacteria (Gram-positive bacteria) and Candida.

Formulation example 1
Toothpaste:
(Component) Mass %
1. Calcium hydrogen phosphate 40.0
2. Glycerin 20.0
3. Carrageenan 1.0
4. Sodium Lauryl Sulfate 1.5
5. Saccharin 0.2
6. Abies sachalinensis extract of Production Example 1 1.0
7. Chlorhexidine digluconate 0.1
8. Preservative (Butyl parahydroxybenzoate) Appropriate amount 9. Perfume (Lemon water) Appropriate amount 10. Colorant Appropriate amount 11. Purified water Balance

Formulation example 2
toothpaste
(Component) Mass %
1. Calcium hydrogen phosphate 30.0
2. Glycerin 20.0
3. Cetylpyridinium chloride 0.2
4. Sodium Lauryl Sulfate 1.5
5. Saccharin sodium 0.1
6. Abies sachalinensis extract of Production Example 2 2.0
7. Sodium monofluorophosphate 4.0
8. Preservative (Methylparaben) Appropriate amount 9. Perfume (Orange water) Appropriate amount 10. Colorant Appropriate amount 11. Purified water Balance

Formulation example 3
Mouthwash:
(Component) Mass %
1. Ethanol 20.0
2. Saccharin sodium 0.1
3. Sodium fluoride 0.1
4. Ascorbic acid 0.1
5. Sodium pyrithione 0.5
6. Benzethonium chloride 0.1
7. Surfactant (sucrose fatty acid ester) 3.0
8. Abies sachalinensis extract of Production Example 1 1.0
9. Preservative (Methylparaben) Appropriate amount 10. Perfume (mint water) Appropriate amount 11. Colorant Appropriate amount 12. Purified water Balance

Formulation example 4
Mouth freshener:
(Component) Mass %
1. Ethanol 20.0
2. Glycerin 15.0
3. Polyoxyethylene hydrogenated castor oil 1.0
4. Saccharin 0.2
5. Chlorhexidine 0.01
6. Abies sachalinensis extract of Production Example 1 1.0
7. Surfactant (Neugen HC-600) 3.0
8. Preservative (Methylparaben) Appropriate amount 9. Coloring agent Appropriate amount 10. Purified water Balance

Formulation example 5
chewing gum:
(Component) Mass %
1. Plate gum base 24.0
2. Starch syrup 13.0
3. Powdered sugar 60.0
4. Todomatsu extract of Production Example 1 1.0
5. Preservative (Methylparaben) Appropriate amount 6. Perfume (Lemon water) Appropriate amount 7. Purified water Balance

Formulation example 6
Candy:
(Component) Mass %
1. Granulated sugar 45.0
2. Abies sachalinensis extract of Production Example 1 1.0
3. Preservative (Methylparaben) Appropriate amount 4. Perfume (Apple water) Appropriate amount 5. Purified water Balance

Formulation example 7
Oral moisturizer:
(Component) Mass %
1. Glycerin 30.0
2. Abies sachalinensis extract of Production Example 1 1.0
3. Xanthan gum Appropriate amount 4. Xylitol Appropriate amount 5. Fragrance (mint) Appropriate amount 6. Purified water Remainder

The present invention is a periodontal disease bacterium inhibitor and an oral composition using a substance that remarkably inhibits periodontal disease bacteria contained in the branches and leaves of a specific conifer. It is highly safe.

Since this component is highly effective against various periodontal disease bacteria, it can be widely used as a quasi-drug, or as a daily toothpaste, mouthwash, gargle, etc. be.

1 ... Microwave distillation device 2 ... Distillation tank 3 ... ... Microwave heating device 4 ... ... Stirring splash 5 ... Air flow inflow pipe 6 ... Distillate outflow pipe 7 ... Cooling device 8 ... Heating control device 9 ... Decompression pump 10 ... Pressure regulating valve 11 ... Pressure control device 12 ... Distillation object

Claims (9)

An agent for suppressing periodontal disease bacteria, characterized by containing, as an active ingredient , an oily fraction of a distillate obtained by heating and distilling Sakhalin fir under reduced pressure . 2. The agent for suppressing periodontal disease bacteria according to claim 1 , wherein the heating is performed by microwave heating. 3. The agent for suppressing periodontal disease according to claim 1 or 2 , which has an inhibitory effect on at least one of Porphylomonas gingivalis and Fusobacterium nucleatum. An oral composition comprising an oily fraction of a distillate obtained by heating and distilling Sakhalin fir as an active ingredient under reduced pressure and an oral composition base. 5. The oral composition according to claim 4 , which is in the form of a toothpaste and contains calcium hydrogen phosphate, glycerin and water as oral composition bases. 5. The oral composition according to claim 4 , which is in the form of a mouthwash and contains ethanol and water as oral composition bases. 5. The oral composition according to claim 4 , which is in the form of an oral moisturizing agent and contains glycerin and water as oral composition bases. A method for inhibiting periodontal disease bacteria, which comprises applying the agent for inhibiting periodontal disease according to any one of claims 1 to 3 in the oral cavity. A method for suppressing periodontal disease bacteria, which comprises applying the oral composition according to any one of claims 4 to 7 to the oral cavity.

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