JP7173817B2 - Cultivation device having a bubble generator for removing carbon dioxide gas - Google Patents

Description

TECHNICAL FIELD The present invention relates to a culture apparatus having a bubble generator for removing carbon dioxide that generates gas such as air for removing carbon dioxide.

In a culture container, for example, when cultures such as animal and plant cells, fungi, and microorganisms are cultured in a medium such as a culture solution, carbon dioxide is generated during the growth of the culture, and this carbon dioxide is When it accumulates in the medium and the carbon dioxide gas concentration in the medium increases, it may adversely affect the growth of the culture.

Therefore, conventionally, a gas for removing carbon dioxide (for moving carbon dioxide) such as air is generated in the contents such as the culture and medium in the culture vessel, and the gas is bubbled. Carbon dioxide gas in the contents is moved (adsorbed) into the gas while the contents are rising, and the carbon dioxide gas is released together with the gas from the liquid surface to a space above the liquid surface, and then cultured. I was trying to drain it out of the container.

FIG. 2 shows a culture apparatus 1 having the conventional air bubble generating section, and 2 is a culture vessel of the culture apparatus 1. The culture vessel 2 is, for example, a bottomed cylindrical vessel that is vertically installed. The culture container 2 contains cultures such as animal and plant cells, bacteria, and microorganisms, and a medium such as a culture solution.

Reference numeral 3 denotes a gas generator. The gas generator 3 includes a bubble generating portion (sparger) 3a made of, for example, sintered metal or the like, which releases a bubble-like gas, and air ( and a tubular gas supply passage 3c connecting the bubble generating portion 3a and the gas supply portion 3b. , The bubble generating part 3a is provided near the bottom of the culture container 2, for example.

In order to discharge the carbon dioxide gas contained in the contents of the culture vessel 2, the gas from the gas supply part 3b is supplied to the bubble generating part through the gas supply channel 3c. 3a, and bubble-like gas is released into the contents from the bubble generating portion 3a. As a result, while the gas rises in the contents, the carbon dioxide gas in the contents moves to the gas, and the carbon dioxide gas is released from the liquid surface of the contents into the upper space along with the gas. Then, the gas is discharged to the outside of the culture container 2 from the gas discharge port 4 formed in the ceiling portion 2a of the culture container 2. As shown in FIG.

In addition, 5 indicates stirring means such as a vertical motion stirring device for stirring the contents in the culture container 2 . Incidentally, the stirring means 5 may be omitted.

Japanese Unexamined Patent Publication No. 56-92783

However, when air bubbles are released into the contents of the culture vessel 2, there is a risk that the culture in the contents may be damaged when the air bubbles burst.

The present invention is intended to provide a culture apparatus that solves these problems.

In order to achieve the above object, the culture apparatus having an air bubble generating section for carbon dioxide removal according to the present invention comprises a culture vessel for containing a culture and a medium, and a carbon dioxide removal device inserted into the culture vessel. and a desired shape into which the bubble-generating part is inserted from the content consisting of the culture and the medium provided in the culture container, which is provided in the culture container. The space is partitioned by a partition wall configured entirely or partially by a mesh-like body through which only the culture medium can pass , the partition wall extending in the vertical direction and having an upper part It is characterized by comprising an open bottomed cylindrical body, and the upper part of the cylindrical body is provided at a position higher than the liquid surface in the culture vessel.

Further, the culture apparatus having an air bubble generating section for carbon dioxide removal of the present invention includes a culture container for containing a culture and a medium, and a culture container inserted into the culture container for generating gas for carbon dioxide removal. and a space part of a desired shape into which the bubble generating part is inserted from the content consisting of the culture and the medium provided in the culture container. Alternatively, a part of the partition wall is composed of a mesh-like body through which the culture medium does not pass, and the partition wall is composed of a vertical planar body, and the planar body is provided at a position higher than the liquid surface in the culture vessel .

According to the present invention, carbon dioxide gas can be removed without damaging the culture in the culture vessel.

1 is a vertical cross-sectional view of a culture apparatus having a gas generating section for carbon dioxide removal according to the present invention. FIG. FIG. 2 is a longitudinal sectional view of a conventional culture apparatus having a gas generating section for removing carbon dioxide.

An example of the mode for carrying out the present invention is shown below.

Embodiment 1 of the present invention will be described with reference to FIG.

In the present invention, as shown in FIG. 1, in the culture container 2 of the conventional culture apparatus 1, from the contents consisting of the culture and medium contained in the culture container 2, A partition wall 6 is provided for partitioning a space portion of a desired shape (size) into which the bubble generating part 3a is inserted, and the partition wall 6 divides the inside of the culture container 2 into a culture and a medium. It is partitioned into one chamber 7 in which objects are contained and the other chamber 8 forming the space portion of the desired size, and the culture passes through the whole or part of the partition wall 6. It is formed by, for example, a mesh-like body (filter) 9 made of stainless steel through which only the medium can pass.

In addition, the partition wall 6 separates the culture container 2 from the other chamber 8 with or without a space 10 above the liquid surface of the contents contained in the culture container 2 . , so that the gas generated in the other chamber 8 is discharged to the outside of the culture container 2 through the gas discharge port 4 .

For example, as shown in FIG. 1, the partition wall 6 is formed of a bottomed cylinder extending vertically and having an open top or an upper end. For example, it is fixed to and supported by the culture container 2 or the gas supply channel 3c fixed to the culture container 2 so as to be higher than the liquid level of the contents contained in the culture container 2 .

The upper opening of the cylindrical body may be airtightly connected to the gas outlet 4 of the culture container 2 .

Then, the bubble generating part 3a of the gas generator 3 is inserted into the other room 8 partitioned by the partition wall 6. As shown in FIG.

For example, the stirring means 5 is provided in one room 7 partitioned by the partition wall 6 . Note that the stirring means 5 may be omitted.

In addition to forming the partition wall 6 by a cylindrical body with a bottom extending in the vertical direction, for example, the partition wall 6 may be formed by a planar body (not shown) such as a vertically installed plate, and the vertically installed planar body The inside of the culture container 2 may be partitioned into one chamber 7 and the other chamber 8 in the left-right direction by the body.

Further, the partition wall 6 is formed by a planar body (not shown) such as a horizontally disposed plate body, and the horizontally disposed planar body vertically separates one room 7 from the other room. It may be divided into eight. In such a case, it is preferable to make the upper room the other room 8 so that the gas generated in the other room is discharged, for example, from the gas discharge port 4 provided in the ceiling part 2a of the culture vessel 2. preferable.

Next, the operation and effects of the culture apparatus of the present invention will be described.

For example, a desired amount of a content consisting of a culture and a medium is supplied into the one chamber 7 from a content supply pipe (not shown). At this time, the liquid surface of the contained contents should not exceed the upper end of the partition wall 6 .

As a result, only the medium among the contents in the one chamber 7 enters the other chamber 8 through the mesh-like body 9, and the culture medium in the one chamber 7 is A content consisting of a substance and a culture medium is contained, and the said other chamber 8 is adapted to contain only a culture medium.

Then, only the culture medium comes and goes between the one chamber 7 and the other chamber 8 via the mesh-like body 9 .

Then, while stirring the content in one chamber 7 of the culture container 2 with the stirring means 5, the culture is cultured for a desired time.

Then, while stirring the contents in the culture vessel 2 with the vertical motion stirring device 5 or after stopping the stirring, in order to remove the carbon dioxide gas accumulated in the medium in the culture vessel 2, the The gas from the gas supply unit 3b is supplied to the bubble generation unit 3a through the gas supply flow path 3c, and bubbles are generated from the bubble generation unit 3a into the contents of the other chamber 8. gas is released.

While the gas rises in the contents, the carbon dioxide gas in the contents moves to the gas, and the carbon dioxide gas is released from the liquid surface of the contents into the upper space part 10 together with the gas. Then, the gas is discharged to the outside of the culture container 2 from the gas discharge port 4 formed in the ceiling portion 2a of the culture container 2. As shown in FIG.

Then, the culture medium from which the carbon dioxide gas has been removed in the other room 8 enters the one room 7, and the culture medium in which the carbon dioxide gas is accumulated in the one room 7 flows into the other room. 8, the carbon dioxide gas in the medium is removed, and the medium from which the carbon dioxide gas has been removed enters the one chamber 7, and by repeating this, the culture container 2 Carbon dioxide in the medium will be removed.

Since the bubbles for carbon dioxide removal are generated only in the other chamber 8 and there are no bubbles for carbon dioxide removal in the one chamber 7, the bubbles do not come into contact with the culture. , the culture will not be damaged.

INDUSTRIAL APPLICABILITY The culture apparatus of the present invention is used as a culture apparatus for medical products, food, and the like.

1 culture device 2 culture vessel 2a ceiling 3 gas generator 3a bubble generator 3b gas supply unit 3c gas supply channel 4 gas outlet 5 stirring means 6 partition wall 7 one room 8 the other room 9 mesh body 10 space Department

Claims (2)

a culture vessel containing a culture and a medium;
a bubble generator for generating gas for removing carbon dioxide, which is inserted into the culture vessel;
Whole or part of a space portion having a desired shape into which the air bubble generating section is inserted is partitioned from a content consisting of a culture and a medium provided in the culture vessel It consists of a partition wall made of a mesh-like body that allows only the medium to pass through, without allowing the culture to pass through,
The partition wall consists of a bottomed cylindrical body with an open top that extends in the vertical direction,
A culture apparatus having an air bubble generator for removing carbon dioxide gas , wherein the upper portion of the cylindrical body is provided at a position higher than the liquid surface in the culture vessel . a culture vessel containing a culture and a medium;
a bubble generator for generating gas for removing carbon dioxide, which is inserted into the culture vessel;
Whole or part of a space portion having a desired shape into which the air bubble generating section is inserted is partitioned from a content consisting of a culture and a medium provided in the culture vessel It consists of a partition wall made of a mesh-like body that allows only the medium to pass through, without allowing the culture to pass through,
The partition wall is composed of a vertical planar body,
A culture apparatus having an air bubble generator for removing carbon dioxide gas , wherein the upper portion of the planar body is provided at a position higher than the liquid surface in the culture vessel .

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