JP7201480B2 - Physical enhancer for plants - Google Patents

Description

TECHNICAL FIELD The present invention relates to a plant body physical strengthening agent containing acetic acid or a salt thereof as an active ingredient.

"Vinegar" is a control material designated as a specific control material (commonly known as "specific agricultural chemicals") stipulated in the Agricultural Chemicals Regulation Law. It is known to have a bactericidal effect. It is believed that the effect against plant diseases is due to the drastic decrease in the pH of the plant caused by the treatment, resulting in the death of pathogenic fungi. In addition, since vinegar has a strong penetrating power, it exerts a control effect against pathogenic bacteria existing in plant tissues. has been found to be effective against
In recent years, as users have become more safety conscious, the need for agricultural chemical materials derived from natural products has increased, and the use of "vinegar" as a control material has attracted attention. For example, it has been proposed to use a post-harvest disease control agent containing vinegar and phosphoric acid as active ingredients to prevent post-harvest spoilage of agricultural products (Patent Document 1). In addition, it has been proposed to attract flying pests such as flies by using vinegar and fragrance as attracting ingredients (Patent Document 2).
On the other hand, since "vinegar" is designated as a specific control material, it is publicly known that there is no risk of harming humans, livestock and aquatic animals and plants. It has not yet been clarified how this will affect

JP 2017-165654 A JP 2013-151470 A

An object of the present invention is to clarify the effect of acetic acid, which is the main component of "vinegar," on plant bodies, and to provide an agricultural chemical material that utilizes this effect.

There has been no attempt to focus on the effect of acetic acid, which is the main component of "vinegar", on plants. The inventors have found that the plant body is physically strengthened by applying it, and have completed the present invention.

In detail, the gist of the present invention is as follows.
1. A physical strengthening agent for plants containing acetic acid or a salt thereof as an active ingredient.
2. 1. Physically reinforcing the epidermis of plant foliage and/or roots; Physical strengthening agent for the plant according to 1.
3.1. A method for physically strengthening a plant, which comprises applying the physical plant strengthening agent described above to the plant.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to physically strengthen the epidermis of a plant, particularly the foliage and roots of the plant.
Due to the physical strengthening action of the plant body in the present invention, the amount of food intake by pests such as sucking and root mastication can be reduced, and the number of offspring of pests can be reduced, so that the damage caused by pests can be greatly suppressed. Furthermore, pathogenic bacteria and nematode infections can also be controlled.
INDUSTRIAL APPLICABILITY According to the present invention, it is possible to obtain a new control effect of suppressing damage to pests without using chemically synthesized agricultural chemicals, which is useful.

It is a graph which shows the result of the "confirmation test of the number of pest broods" in an Example. It is a graph which shows the result of the "confirmation test of the pest colonization inhibitory effect" in an Example.

The present invention will be described in detail below.
<About physical strengthening of the plant body>
The physical strengthening of the plant body of the present invention means that the epidermis of the foliage and root parts of the plant body is strengthened, and the whole plant body is physically strengthened. In this specification, the physical strength of the present invention is expressed in terms of physical measurements such as tensile strength and punch strength of plant leaves, as will be described in detail with test examples below.

<About acetic acid or its salt>
The active ingredient of the physical strengthening agent for plants of the present invention is acetic acid or a salt thereof.
Acetic acid is a carboxylic acid represented by the molecular formula C ₂ H ₄ O ₂ , a weak acid contained in vinegar, and a compound with a strong sour taste and a pungent odor. Acetic acid that can be used in the present invention includes not only pure acetic acid, but also brewed vinegar and synthetic vinegar, which are table vinegars. These are commercially available, and for example, grain vinegar, special vinegar, highly concentrated brewed vinegar, and powdered vinegar (a mixture of acetic acid and dextrin, etc.) can be used. Fruit vinegars such as wine vinegar and apple vinegar are also available.
Examples of acetic acid salts include sodium salts, potassium salts, calcium salts, magnesium salts, ammonium salts, ethanolamine salts, triethanolamine salts, etc. As the active ingredient of the plant physical strengthening agent of the present invention, acetic acid When salts are used, sodium, triethanolamine, ammonium and potassium salts are preferred. These salts may be added as a single substance into the physical enhancer for the plant body, but acetic acid and the corresponding neutralizing agent may be added separately to form the salts during preparation of the formulation. For example, acetic acid and sodium hydroxide as a neutralizing agent can be added separately and used as the sodium salt. The neutralizing agent is not particularly limited as long as it neutralizes the acidity of acetic acid, and examples thereof include sodium hydroxide and potassium hydroxide. As for the physical strengthening agent for plants of the present invention, those containing acetic acid or a salt thereof may be used singly or in combination of two or more.

The plant physical strengthening agent of the present invention contains acetic acid or a salt thereof as an active ingredient, preferably 0.01% by weight or more, more preferably 0.05% by weight or more, of the total plant body physical strengthening agent, More preferably, it can be used in an amount of 0.08% by weight or more. In addition, if too much acetic acid or a salt thereof is used, some users may be concerned about the acetic acid odor, so the content is preferably 10% by weight or less, more preferably 5% by weight or less, and 3% by weight or less. is more preferred.
The plant body physical enhancer of the present invention can be applied to the plant body as it is, but the plant body can also be treated by diluting a formulation containing a predetermined active ingredient with water at the time of use. In this case, the content of the active ingredient acetic acid or a salt thereof in the preparation diluted with water is preferably 0.01% by weight or more, more preferably 0.05% by weight or more, and still more preferably 0.08%. It can be prepared and used so that it becomes more than weight%.

The physical strengthening agent for plants of the present invention can be used as various formulations.
Examples of formulations include oils, emulsions, wettable powders, flowables (suspensions in water, emulsions in water, etc.), microcapsules, powders, granules, tablets, liquids, sprays, aerosols, and the like. be done. Among them, formulations for atomization such as sprays and aerosols, dusting agents in which a liquid agent is filled in a container with a watering can head, etc., can maximize the performance of the physical strengthening agent for plants of the present invention. It is suitable as a formulation type that can be used. In order to prepare a spray or aerosol, an aerosol can or a drug bottle having a predetermined spray pattern and a spray device for supplying spray particles can be used.
The physical strengthening agent for plants of the present invention is not limited to liquid formulations, and can also be used as solid formulations such as powders, granules, and fine particles as long as the effects of the present invention are exhibited.
As one production example of the above formulation, acetic acid and, if necessary, a surfactant are used to dissolve in a solvent to prepare a solution (A solution), and this A solution is mixed with an appropriate amount of water and stirred to form a formulation. can be used as a physical strengthening agent for plants that does not need to be diluted at the time of use.
As water, tap water, ion-exchanged water, distilled water, filtered water, sterilized water, underground water, or the like can be used.

Examples of liquid carriers used for formulation include alcohols (methanol, ethanol, isopropyl alcohol, butanol, hexanol, benzyl alcohol, ethylene glycol, etc.), ethers (diethyl ether, ethylene glycol dimethyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, propylene glycol monomethyl ether, tetrahydrofuran, dioxane, etc.), esters (ethyl acetate, butyl acetate, isopropyl myristate, ethyl lactate, etc.), ketones (acetone, methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone, etc.), aromatic or fatty Group hydrocarbons (xylene, toluene, alkylnaphthalene, phenylxylylethane, kerosene, light oil, hexane, cyclohexane, etc.), halogenated hydrocarbons (chlorobenzene, dichloromethane, dichloroethane, trichloroethane, etc.), nitriles (acetonitrile, isobutylene, etc.) ronitrile, etc.), sulfoxides (dimethylsulfoxide, etc.), heterocyclic solvents (sulfolane, γ-butyrolactone, N-methyl-2-pyrrolidone, N-ethyl-2-pyrrolidone, N-octyl-2-pyrrolidone, 1, 3-dimethyl-2-imidazolidinone), acid amides (N,N-dimethylformamide, N,N-dimethylacetamide, etc.), alkylidene carbonates (propylene carbonate, etc.), vegetable oils (soybean oil, cottonseed oil, etc.), plants Essential oils (orange oil, hyssop oil, peppermint oil, lemon oil, etc.) and water.

Surfactants used in formulation include nonionic surfactants, anionic surfactants, cationic surfactants, and amphoteric surfactants. Nonionic surfactants include, for example, polyoxyalkylene allylphenyl ether, polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene allylphenyl ether, polyoxyethylene styrylphenyl ether, polyoxyethylene alkylphenyl Ether formaldehyde condensates, polyoxyethylene-polyoxypropylene block polymers, polyoxyethylene-polyoxypropylene block polymer alkylphenyl ethers, sorbitan fatty acid esters (e.g., sorbitan monooleate, sorbitan laurate), polyoxyethylene fatty acid esters, Glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene hydrogenated castor oil, polyethylene glycol fatty acid ether, and the like. Anionic surfactants include, for example, alkyl sulfates, polyoxyethylene alkyl ether sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene benzyl (or styryl)phenyl ether sulfates or polyoxyethylene-polyoxypropylene block polymers. Sodium, calcium or ammonium sulfate salts; alkyl sulfonates, dialkyl sulfosuccinates, alkyl benzene sulfonates (e.g. calcium dodecylbenzene sulfonate), mono- or di-alkyl naphthalene sulfonates, naphthalene sulfonate formaldehyde condensation sodium, calcium, ammonium or alkanolamine salts of lignosulfonic acid, polyoxyethylene alkylphenyl ether sulfonic acid or polyoxyethylene alkyl ether sulfosuccinate; polyoxyethylene alkyl ether phosphate, polyoxyethylene, mono- or salts such as sodium or calcium salts of di-alkylphenyl ether phosphate, polyoxyethylene benzyl (or styryl) phenyl ether phosphate, polyoxyethylene-polyoxypropylene block polymer phosphate. Examples of cationic surfactants include quaternary ammonium salts, alkylamine salts, alkylpyridinium salts, alkyl oxides and the like. Amphoteric surfactants include, for example, alkylbetaines and amine oxides.

Examples of the propellant used when forming an aerosol include butane gas, freon gas, substitute freon (HFO, HFC, etc.), liquefied petroleum gas (LPG), dimethyl ether, and carbon dioxide gas.
Examples of solid carriers include clays (kaolin, diatomaceous earth, bentonite, clay, acid clay, etc.), synthetic hydrated silicon oxide, talc, zeolite, ceramics, and other inorganic minerals (sericite, quartz, sulfur, activated carbon, carbonic acid, etc.). calcium, hydrated silica, etc.), porous bodies, and the like.

Antifoaming agents, preservatives, antioxidants, thickeners, and the like can be added to the physical strengthening agent for the plant body of the present invention, if necessary, at the time of preparation of the formulation.
Examples of antifoaming agents include silicone antifoaming agents and fluorine antifoaming agents.
Examples of antiseptics include organic nitrogen sulfur compounds, organic bromine compounds, isothiazoline compounds, benzyl alcohol mono(poly)hemiformal, 1,2-benzisothiazolin-3-one, 5-chloro-2-methyl- 4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one, 2-bromo-2-nitropropane-1,3-diol, potassium sorbate, sodium dehydroacetate and the like.
Antioxidants include, for example, tetrakis[methylene-3-(3,5-di-t-butyl-4-hydroxyphenyl)propionate]methane, butylated hydroxytoluene (BHT), butylhydroxyanisole (BHA), gallic propyl acid, and vitamin E, mixed tocopherols, α-tocopherols, ethoxyquin and ascorbic acid, and the like.
Examples of thickeners include polyvinylpyrrolidone, xanthan gum, polyvinyl alcohol, guar gum, carboxyvinyl polymer and the like.

<Regarding pest control effect>
The physical strengthening agent for plants of the present invention exerts an effect of physically strengthening the epidermis of stems, leaves and roots of plants, as described in detail in the following examples.
Here, the feeding behavior of sap-sucking pests such as aphids (1) settles on plants. (2) Probing a pod-like proboscis, touching the surface of a leaf with the proboscis and inserting the proboscis into the proboscis to search for a localization point. (3) Fix the proboscis at the stereotactic site and insert the stylus into the sieve canal inside the plant body. (4) It is generally recognized that it initiates sucking behavior.
For example, when the epidermis of the foliage of the plant body is physically strengthened by the physical strengthening agent for the plant body of the present invention, sucking pests such as aphids can try inserting the needle of (2) above. , or (3) insertion of the mouthpiece becomes difficult, it is considered that feeding by sucking juice becomes difficult, and as a result, the effect of suppressing feeding damage by pests is exhibited. In addition, when the epidermis of foliage and roots of plants is physically strengthened, for example, larvae of scarab beetles chew roots and damage plants. also has a suppressive effect.
Such chewing insect pests include, for example, Coleoptera such as weevils, leaf beetles, scarab beetles, scarab beetles, and Japanese beetles; Thrips, Spodoptera, Aphids, Uwaba, Helicopter moth, Poison moth, Brown moth, Mei moth, Corbicula spp., Noctuid moth, Inchworm, Lepidoptera such as black moth, Chickbug, Sparrow moth, Diamondback moth, Hair beetle, Diptera such as leafminer, fruit fly, seed fly, spider mites, acarids , Acarina such as rust mites and dust mites, Hymenoptera such as sawflies, and their larvae.
In addition, although different from pests, plant parasitic nematodes such as root-knot nematode, Negusare nematode, and cyst nematode, which are parasitic on the roots of crops, also spread infection by inserting the needle into the root of the plant body. The plant body physical strengthening agent also exerts an effect of suppressing nematode damage.
It is known that pathogenic fungi invade the plant body by forming appressorium that attaches to the plant by means of germ tubes generated from spores and inserting invasive hyphae into the plant from the tip of this appressorium. When the epidermis of foliage and roots of a plant is physically strengthened by the physical strengthening agent of the present invention, it is difficult to either form an appressorium that attaches to the plant or insert an invading hypha. As a result, it is considered that pathogenic fungi are difficult to enter the plant body, and as a result, the effect of suppressing plant diseases is exhibited. Such plant diseases include those caused by filamentous fungi, such as powdery mildew, gray mold, scab, downy mildew, blast, white rust, and brown spot.

<About plants>
The plant body in the present invention is not limited as long as it can be physically strengthened. Specifically, for example, crops whose leaves are used (cabbage, tea, tobacco, herbs such as mint and basil, etc.), crops whose stems are used (asparagus, udo, etc.), roots or rhizomes are used crops (sweet potatoes, potatoes, etc.), crops whose flowers are used (roses, pansies, chrysanthemums and other flowering plants, hops, saffron, etc.), crops whose seeds are used (wheat, corn, soybeans, etc.), fruits are used (cucumbers, green peppers, tomatoes, grapes, bananas, mandarin oranges, apples, blueberries and other fruit trees).
As long as the physical strengthening agent of the present invention can be attached to the plant body, there is no restriction on the part of the plant body to which it is attached. Application is preferred, and application to the surface of plant leaves is particularly preferred. Specifically, the plant only needs to be sufficiently wetted, and for example, it is preferable to treat a plant body of 10 to 60 cm with 10 mL or more. The timing of application may be appropriately selected according to the growing conditions of the plant. The frequency of application is once every 1 to 10 days, preferably once every 1 to 7 days, and more preferably once every 1 to 4 days. Application means are not particularly limited.
The treatment amount of the physical strengthening agent for the plant body of the present invention is 0.005 g/week as an integrated treatment amount for the plant body, based on the active ingredient acetic acid or a salt thereof, regardless of the frequency of application. Above, preferably 0.01 g/week or more, more preferably 0.02 g/week or more is applied. In particular, for plants with a height of 10 to 60 cm above the ground, the cumulative treatment amount may be applied in the range of 0.02 g/week or more and 10 g/week or less, preferably 0.05 g/week or more and 7 g/week or less. range, more preferably 0.1 g/week or more and 5 g/week or less.

In addition, depending on the purpose, for example, bactericides, antifungal agents, insecticidal and acaricidal agents, repellents, fragrances, essential oils and the like may be used in combination. Bactericides such as bitertanol, bromconazole, cyproconazole, difenoconazole, hexaconazole, imazalil, microbutanil, simeconazole, tetraconazole, thiabendazole, penthiopyrad, mancozeb; benzethonium chloride, benzalkonium chloride, chlorhexidine hydrochloride, glucon Antifungal agents such as chlorhexidine acid, hinokitiol, phenoxyethanol, isopropylmethylphenol; pyrethrum extract, natural pyrethrin, prallethrin, imiprothrin, phthalthrin, allethrin, bifenthrin, resmethrin, phenothrin, cyphenothrin, permethrin, cypermethrin, etofenprox, cyfluthrin, deltamethrin , Bifenthrin, Fenvalerate, Fenpropathrin, Empentrin, Silafluofen, Transfluthrin, Metofluthrin, Profluthrin and other pyrethroid compounds; Carbaryl, Propoxur, Methomyl, Thiodicarb and other carbamate compounds; Methoxadiazone and other oxadiazole compounds; Fipronil Phenylpyrazole compounds such as phenylpyrazole compounds, sulfonamide compounds such as amidoflumet, neonicotinoid compounds such as dinotefuran and imidacloprid, pyrrole compounds such as chlorphenapyr, fenitrothion, diazinon, marathon, pyridafenthion, prothiophos, phoxim, chlorpyrifos, dichlorvos, etc. Insecticides and acaricides such as organophosphorus compounds; DEET, di-n-butylsuccinate, hydroxyanisole, rotenone, ethyl-butylacetylaminopropionate, icaridin (picaridin), 3-(Nn-butyl- One or more repellents such as N-acetyl)aminopropionic acid ethyl ester (IR3535) can be used. As fragrances and essential oils, one or a combination of two or more selected appropriately from the group consisting of natural fragrances, synthetic fragrances, natural extracts and the like can be used depending on the application.

EXAMPLES The present invention will be described in more detail with reference to examples below, but the present invention is not limited to these.
In the examples, parts means parts by weight unless otherwise specified.

<Confirmation test 1 of the physical strengthening action of the plant body>
(1) Test sample Example 1
Using 0.25 parts by weight of acetic acid, 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monooleate) and purified water, a total amount of 100 parts by weight was prepared as a physical strengthening agent for plants.
Comparative example 1
A comparative agent was prepared using 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monooleate) and purified water to bring the total amount to 100 parts by weight.

(2) Confirmation test method for physical strengthening action of plant body As a test plant, a No. 3 pot was used, and a cabbage that had been sown for 30 days and a above-ground part grown in a No. 10 pot were about 60 cm. I used roses.
For 2 weeks, the test specimen (Example 1, Comparative Example 1) was applied to the test plant 13 times/2 weeks for cabbage and 3 times/2 weeks for roses, and the sprayer was applied to the extent that the entire test plant was sufficiently wet. was sprayed using
Two weeks after the end of spraying treatment, leaf blade portions of each test plant (cabbage: approximately 30 cm ² , rose: approximately 50 cm ² ) were used to measure tensile strength and punch strength with a force gauge.
The tensile strength in this test means the tensile strength in the main vein direction of the leaf veins. A force gauge measuring device was clamped at a position 1 cm from both ends of the leaf segment (distance between clamps: about 3 to 7 cm) to measure the cutting strength. The test was carried out 4 times on cabbage and 5 times on roses, each using leaf blade parts taken from different test plants.
The punch strength in this test was determined by placing a force gauge at the center of the leaf section and measuring the strength when the leaf penetrated. The test was carried out 3 times on cabbage and 5 times on roses, each using leaf blade parts taken from different test plants.
The average tensile strength and punch strength measurements are listed in Table 1 along with the composition of the test specimens.

(3) Results As shown in Table 1, the plant physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention gave approximately It was confirmed that it was improved by about 1.4 times. From this test result, it was clarified that the agent for physically strengthening plants of the present invention exerts the effect of physically strengthening plants.
In addition, when the tensile strength in the direction orthogonal to the main veins of cabbage was confirmed, it was confirmed that there was a tendency to strengthen by a maximum of 1.5 times.

<Confirmation test 2 of the physical strengthening action of the plant body>
Two weeks after spraying the test specimens (Example 1, Comparative Example 1) of the above "Confirmation Test 1 for Physical Strengthening of Plant Body", leaf blade portions of cabbage (approximately 30 cm ² ) were used, A confirmatory test was performed on the epidermis.
The epidermis in this test was measured with a digital microscope (VHX-900 manufactured by KEYENCE), and the ratio of the thickness of the epidermis to the thickness of the cross section of the section was calculated by the following formula.
Calculation formula: Epidermis ratio (%) = thickness of epidermis / thickness of cross section of section x 100
The test was performed three times, and Table 2 shows the average values of the cross-sectional thickness (μm) of the section, the epidermis thickness (μm) and the epidermis ratio (%). In Table 2, "cross-section thickness" is the thickness of the cross section of the section (μm), "skin thickness" is the thickness of the epidermis (μm), and "percentage of epidermis" is the epidermis percentage (%) calculated from the above formula. means

As shown in Table 2, the plant body treated with the physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention is compared with the plant body treated with Comparative Example 1 containing no acetic acid. Although the thickness (μm) of the cross section of the section is almost the same, the thickness (μm) of the epidermis is dramatically increased, and it is clear that the epidermis ratio is improved by about 1.7 times. became.

<Confirmation test of pest feeding behavior>
Using a cabbage leaf portion (approximately 30 cm ² ) after spraying the test specimen (Example 1, Comparative Example 1) of the above “Confirmation Test 1 for Physical Strengthening of Plant Body” using a sprayer , confirmed the feeding behavior of pests.
After the cabbage leaves were cut from the seedlings, one test insect (green peach aphid) was released on the leaf blade of the cabbage, and the time during which it took a "probing behavior" with its proboscis raised was called "exploration time (seconds)." , the time during which the sucking position was localized and sucked was defined as "sucking time (seconds)", and each time was measured, and the sum was calculated as "feeding time (seconds)". If the time to stay in one place with the proboscis upright was less than 1 minute, it was defined as "probing behavior", and if it was longer than 1 minute, it was defined as "sucking behavior". Each time was observed and measured with a digital microscope. Observations were made for about 20 minutes.
"Foraging time (seconds)" = "Search time (seconds)" + "Sucking time (seconds)"
In addition, on the center of the top surface of a plastic cup (hereinafter referred to as "KP cup") of 130 mm in diameter and 100 mm in height, on which a filter paper (9 cm in diameter) was laid, was placed a cabbage leaf in which one of the above-mentioned test insects had been released. After 12 hours from attaching the leaf blade, the filter paper was taken out and sprayed with a ninhydrin reagent, and the number of red-colored portions was confirmed. This is a known test method that utilizes the fact that excreta (honeydew) of test insects turns red with a ninhydrin reagent.
The "feeding time (seconds)" on the section was measured twice, and the average value and the number of excrement colored red by the ninhydrin reagent are shown in Table 3 as the "honeydew number" (pieces).

As shown in Table 3, compared with the plant body treated with the plant body physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention, compared with the plant body treated with Comparative Example 1 containing no acetic acid, The ``exploration time (seconds)'' for performing ``probing behavior'' on the leaf blade of cabbage is 4 times longer, and the ``sucking time (seconds)'' is 2 times longer. It was confirmed to be about four times longer. This is because, in the plant body treated with the plant body physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention, it is difficult to secure a place where the juice can be sucked, and it takes time to suck the juice. It was found that the time required to feed the volume was greatly increased.
In addition, in the plant body treated with the plant body physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention, the honeydew number was 1 in 12 hours, and only one excretion was confirmed. Met. From this, it was revealed that the physical enhancer for plant bodies of Example 1 greatly inhibits the feeding behavior of pests compared to Comparative Example 1.
These facts confirm that the plant epidermis was physically strengthened by the plant physical strengthening agent of the present invention, which was confirmed in "Confirmation Tests 1 and 2 of Physical Strengthening of Plants". This is probably due to the fact that it was difficult to pierce with a needle, or that it was difficult to suck the juice itself.

<Confirmation test for the number of offspring produced by pests>
Using a cabbage petiole and leaf blade portion (approximately 35 cm ² ) after spraying the test specimen (Example 1, Comparative Example 1) of the above “Confirmation Test 1 for Physical Strengthening of Plant Body” using a sprayer Then, a confirmatory test was conducted on the number of offspring produced by pest insects.
The petiole was placed in a water-filled container so that the leaf blade portion of the cabbage would not dry out in a KP cup, and one winged test insect (green peach aphid) was released on the leaf blade. The number of green peach aphids on the leaf blade of cabbage was counted every number of days shown in FIG. The test was conducted twice using petioles and leaf blades collected from different test plants, respectively, and the average cumulative head count is shown in FIG. 1 as the litter size.

As shown in FIG. 1, the cumulative number of offspring on the plant body treated with the physical strengthening agent of Example 1 containing acetic acid as an active ingredient of the present invention is the same as that of Comparative Example 1 that does not contain acetic acid. Compared to the cumulative number of offspring produced on the plant body tested, it was found to be greatly suppressed, and on the 19th day from the start of the test, the number was about one-third.
As is clear from the above-mentioned "confirmation test of feeding behavior of insect pests", the plant body treated with the physical strengthening agent of the present invention has its epidermis physically strengthened, making feeding difficult. , which is thought to have induced a decrease in litter size.

<Confirmation test of pest colonization inhibitory effect>
(1) Test sample Example 2
Using 0.125 parts by weight of acetic acid, 0.08 parts by weight of a spreading agent (polyoxyethylene sorbitan monooleate) and purified water, a total amount of 100 parts by weight was prepared as a physical strengthening agent for plants.
In Examples 3 and 4 and Comparative Example 1, each test sample was obtained in the same manner as in Example 2 with the formulation shown in Table 4 below.

(2) Confirmation Test Method for Effect of Inhibiting Pest Settlement As test plants, green peach aphids were released on miniature roses with a height of about 20 cm grown in a No. 3.5 pot.
Test specimens (Examples 2 to 4, Comparative Example 1) were applied to the test plants for 2 weeks at two different treatment frequencies (treatment a twice every 2 weeks, treatment b every 2 weeks). The treatment frequency was 7 times), and the spray was applied using a sprayer to such an extent that the entire test plant was sufficiently wet. The number of green peach aphids on the test plant was counted every number of days elapsed from the first day of spraying shown in FIG. Each test was conducted twice, and the average cumulative number of insect pests is shown in FIG. For example, "Example 2a" in FIG. 2 and Table 5 means test data obtained by using Example 2 as a test sample and dispersing it at the treatment frequency of treatment a.

(3) Results As shown in FIG. 2 and Table 5, in treatment b, which is a treatment frequency of 7 times in 2 weeks, the physical strengthening agent for plants of Examples 2 to 4 containing acetic acid of the present invention as an active ingredient When treated (Examples 2b, 3b, 4b), compared to the case of Comparative Example 1 containing no acetic acid (Comparative Example 1b), it is clear that the cumulative number of pests on the plant is greatly suppressed. became. Examples 2 and 3, which contain 0.125% by weight and 0.1% by weight of acetic acid as an active ingredient, exhibit extremely excellent pest control effects ( Examples 2a, 3a) were confirmed.
In particular, as shown in FIG. 2, it became clear that the insect pest colonization inhibitory effect of the physical strengthening agent for the plant of the present invention is extremely excellent after 15 days have passed since the treatment of the test specimen was stopped. rice field.

<Confirmation test of antibacterial effect>
As a test plant, a miniature rose grown in a No. 3.5 pot with a height of 20 cm above the ground was used.
Using the test specimens (Examples 2 to 4, Comparative Example 1) of the above-mentioned "confirmation test of pest colonization inhibitory effect", a test was conducted to confirm the antibacterial effect.
The test specimens (Examples 2 to 4, Comparative Example 1) were applied to the test plants for 2 weeks at a treatment frequency of 7 times in 2 weeks using a sprayer to the extent that the entire test plants were sufficiently wet. bottom. After completion of the treatment, powdery mildew fungus spores collected from the lesions of separately prepared powdery mildew-infected strains were applied to the test plants, and the presence or absence of disease onset was confirmed 2 weeks later. Table 6 shows the test results. "-" in Table 6 means that the disease did not occur, and "+" means that the disease occurred.

As shown in Table 6, the physical strengthening agents for plants of Examples 2 to 4 containing acetic acid as an active ingredient of the present invention did not develop disease despite being inoculated with powdery mildew. In contrast, powdery mildew was confirmed in the plants treated with Comparative Example 1 containing no acetic acid.
As is clear from the above-mentioned "confirmation test of feeding behavior of insect pests", this result shows that the epidermis of plants treated with the physical strengthening agent of the present invention was physically strengthened, so that the epidermis adhered to the plants. It is considered that either the formation of the vessel or the insertion of the invading mycelium became difficult, and pathogenic fungi could not invade the plant body, and as a result, the onset of plant diseases was suppressed.

The physical strengthening agent for plants of the present invention can strengthen the epidermis of plants, particularly stems and leaves and roots of plants. This strengthening of the epidermis is thought to be due to an increase in the amount of the components that make up the epidermis in the plant body, since the thickness of the epidermis is increased and both the tensile strength and punch strength are improved.
As a result, when the plant body is applied with the plant body physical strengthening agent of the present invention, the epidermis of the foliage part and the root part of the plant body is particularly physically strengthened, and the amount of food intake by pests such as sucking juice and root mastication is reduced. In addition, since the number of offspring of pests can also be reduced, pest damage can be greatly suppressed. Furthermore, pathogenic bacteria and nematode infections can also be prevented.
INDUSTRIAL APPLICABILITY According to the present invention, it is possible to obtain a new control effect of suppressing damage to pests without using agricultural chemicals, which is extremely useful.

Claims (2)

Physical strengthening of plants (excluding rice, cut flowers and cherry tomatoes) characterized by physically strengthening the epidermis of plant foliage and/or roots using acetic acid or a salt thereof as an active ingredient. agent. The physical strengthening agent for plant bodies according to claim 1 is applied to plants (excluding rice, cut flowers or cherry tomatoes) , and the epidermis of plant stems and leaves and / or roots is physically How to strengthen.

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