JP7207435B2 - Filters and liquid chromatographs used in liquid chromatographs - Google Patents

Description

The present invention relates to filters and liquid chromatographs used in liquid chromatographs.

2. Description of the Related Art In liquid chromatographs, filters such as suction filters and line filters are used to remove foreign matter from an eluent flowing through a channel. These filters remove micron-order-sized contaminants mixed in the eluent. These filters are provided at a location where the eluent is sucked from the eluent tank, a liquid feed pump, a mixer, an autosampler, a column, or the like.

A sintered body of metal, ceramics, or the like is used as the filter. In the manufacturing process of sintered bodies such as metals and ceramics, sintering aids called binders are used for the purpose of improving the adhesion between materials, or for the purpose of adjusting filter characteristics such as pore size or shape. . Patent Document 1 listed below discloses a sintered metal filter obtained by sintering a mixture of a metal powder and an organic binder. As the binder, for example, an organic material such as polyethylene or polypropylene is used. Alternatively, an inorganic material is used as the binder. Further, Patent Document 2 below discloses a column filter. Metal spheres, glass spheres, ceramic spheres, or synthetic resin spheres are used as the material constituting this column filter.
JP 2011-179077 A JP 2018-4489 A

The binder is removed during the manufacturing process of the sintered body filter. However, it is difficult to completely remove the binder, and the unremoved binder remains in the sintered filter. Therefore, when a sintered filter is used in a liquid chromatograph, the binder remaining in the filter is gradually eluted. Then, the binder eluted from the filter may reach the downstream detector.

As a liquid chromatograph detector, for example, an absorbance detector having a quartz flow cell is used. If a quartz flow cell is used in the detector, the inflowing binder will contaminate the quartz flow cell. Contamination of the quartz flow cell causes baseline drift in the chromatogram. Alternatively, contamination of the quartz flow cell causes noise in the chromatogram to be exacerbated. Although it is possible to remove the binder by washing, it is difficult to specify the washing time and the like due to manufacturing variations and the like. Also, analysis processing cannot be performed until the binder is removed by cleaning. Alternatively, if the eluted binder is retained on the column, unintended peaks called ghosts appear in the chromatograph.

A primary object of the present invention is to prevent the binder from flowing into the liquid chromatograph.

The filter used in the liquid chromatograph according to one aspect of the present invention is a filter provided in the flow path of the liquid chromatograph, the filter is composed of a sintered body of a fibrous material that does not use a binder, The body is formed into sheets.

A filter used in this liquid chromatograph is formed by sintering a fibrous material without using a binder. Since the binder does not elute from the filter, it is possible to prevent the binder from flowing into the detector. This prevents baseline drift or ghosting in the chromatogram.

According to the present invention, it is possible to prevent the binder from flowing into the liquid chromatograph.

FIG. 1 is an overall view of a liquid chromatograph according to this embodiment. FIG. 2 is a side view of the suction port and suction filter according to this embodiment. FIG. 3 is a side cross-sectional view of a suction port and a suction filter according to this embodiment. FIG. 4 shows a chromatogram obtained by using the suction filter of the present embodiment and a chromatogram obtained by using the suction filter of the comparative example.

Next, the configuration of a liquid chromatograph according to an embodiment of the present invention will be described with reference to the accompanying drawings.

(1) Overall Configuration of Liquid Chromatograph FIG. 1 is an overall configuration diagram of a liquid chromatograph 10 according to the present embodiment. The liquid chromatograph 10 comprises an eluent tank 1A, an eluent tank 1B, a pump 2A, a pump 2B, a mixer 3, an autosampler 4, a separation column 5 and a detector 6.

The eluent tank 1A contains an eluent 11A. The eluent tank 1B contains an eluent 11B. The liquid chromatograph 10 of the present embodiment can perform gradient elution by supplying two types of eluents 11A and 11B to the separation column 5 while adjusting the mixing ratio thereof.

One end of a channel tube 71A is connected to the eluent tank 1A. The other end of the flow pipe 71A is connected to the pump 2A. A suction filter F1A is provided at one end of the flow pipe 71A connected to the eluent tank 1A. By driving the pump 2A, the eluent 11A in the eluent tank 1A is sent to the flow pipe 72A downstream of the pump 2A through the suction filter F1A and the flow pipe 71A. One end of a channel pipe 71B is connected to the eluent tank 1B. The other end of the flow pipe 71B is connected to the pump 2B. A suction filter F1B is provided at one end of the flow tube 71B connected to the eluent tank 1B. By driving the pump 2B, the eluent 11B in the eluent tank 1B is sent through the suction filter F1B and the flow pipe 71B to the flow pipe 72B downstream of the pump 2B.

The pump 2A is provided with a line filter F2A1 at a location where the flow pipe 71A is connected. Further, the pump 2A is provided with a line filter F2A2 at a location where the flow pipe 72A is connected. By driving the pump 2A, the eluent 11A sucked from the eluent tank 1A flows into the pump 2A via the line filter F2A1. The eluent 11A discharged from the pump 2A is delivered to the flow tube 72A via the line filter F2A2.

The pump 2B is provided with a line filter F2B1 at a location where the flow pipe 71B is connected. Further, the pump 2B is provided with a line filter F2B2 at a location where the flow pipe 72B is connected. By driving the pump 2B, the eluent 11B sucked from the eluent tank 1B flows into the pump 2B via the line filter F2B1. The eluent 11B discharged from the pump 2B is delivered to the flow pipe 72B via the line filter F2B2.

As described above, the liquid chromatograph 10 of this embodiment can perform gradient elution. When performing gradient elution, the liquid feeding amounts of the eluent 11A and the eluent 11B are adjusted by adjusting the liquid feeding patterns (for example, pump pressure or liquid feeding flow rate) of the pumps 2A and 2B. Thereby, the mixed eluent can be supplied to the separation column 5 while changing the mixing ratio of the eluent 11A and the eluent 11B flowing into the mixer 3 with time.

For example, eluent 11A and eluent 11B are eluents containing water, acetonitrile, methanol, and the like. However, the eluent 11A and the eluent 11B differ in the mixing ratio of water, acetonitrile, and methanol. Therefore, by adjusting the liquid feeding pattern (for example, pump pressure or liquid feeding flow rate) of the pump 2A and the pump 2B, the mixing ratio of the eluent 11A and the eluent 11B is changed, so that the mixed eluent contains The proportion of each component can change over time.

Flow pipes 72A and 72B are connected to the mixer 3 . The mixer 3 is provided with line filters F3A and F3B at locations where the flow pipes 72A and 72B are connected. By driving the pumps 2A and 2B, the eluents 11A and 11B sent through the flow pipes 72A and 72B flow into the mixer 3 through the line filters F3A and F3B. The eluents 11A and 11B that have flowed into the mixer 3 pass through the mixing section within the mixer 3 . The eluents 11A and 11B that have passed through the mixing section flow out of the mixer 3 .

The mixer 3 is provided with a line filter F30 at a location where the flow pipe 73 is connected. A mixture of the eluent 11A and the eluent 11B flowing out of the mixer 3 (hereinafter referred to as a mixed eluent) flows out to the flow pipe 73 via the line filter F30. The mixed eluent flowing through the flow tube 73 is supplied to the autosampler 4 .

In the autosampler 4, the injector 41 drops the sample into the mixed eluent in the analysis channel. A line filter F4 is provided inside the injector 41 of the autosampler 4 . A sample delivered from the injector 41 is dropped into the mixed eluent via the line filter F4. The mixed eluent to which the sample has been dropped in the autosampler 4 is sent to the separation column 5 through the channel tube 74 .

A mixed eluent into which a sample is injected is supplied to the separation column 5 . The separation column 5 is provided with a line filter F51 at a location where the flow pipe 74 is connected. The mixed eluent flowing into the separation column 5 through the flow tube 74 passes through the line filter F51. In the separation column 5 , sample separation occurs while the mixed eluent passes through the stationary phase within the separation column 5 . The mixed eluent in which the sample dissolved from the separation column 5 flows out to the channel tube 75 . The separation column 5 is provided with a line filter F52 at a location where the flow pipe 75 is connected. The mixed eluent in which the sample is dissolved flows out to the flow pipe 75 via the line filter F52. The mixed eluent in which the sample is dissolved and flowed out to the channel tube 75 is sent to the detector 6 .

A mixed eluent from which the sample is separated in the separation column 5 is supplied to the detector 6 . The detector 6 is provided with a line filter F6 at a location where the flow pipe 75 is connected. The mixed eluent flowing into the detector 6 through the flow tube 75 passes through the line filter F6. In this embodiment, an absorbance detector is used as the detector 6 . The detector 6 is equipped with a quartz flow cell, and when the mixed eluent containing the sample passes through the flow cell having quartz in the wetted part, the quartz flow cell is irradiated with light of a predetermined wavelength. Then, the sample is detected by measuring the intensity change of the light before and after the quartz flow cell.

(2) Structure of sheet-like filter Next, the structure of the sheet-like filter used in the liquid chromatograph 10 will be described. Here, the suction filter F1A connected to one end of the flow pipe 71A connected to the eluent tank 1A will be described as an example. FIG. 2 is a side view showing the suction port 8 and the suction filter F1A. FIG. 2 is a side sectional view showing the suction port 8 and the suction filter F1A. The suction port 8 is connected to one end of the flow pipe 71A. A suction filter F1A is attached to the end of the suction port 8 .

The suction port 8 is composed of two cylindrical members 81 and 82, as shown in FIGS. A channel pipe insertion hole 811 is formed in the axial center of the cylindrical member 81 . The channel tube insertion hole 811 is a substantially cylindrical hole. The flow pipe 71A is inserted into the flow pipe insertion hole 811. As shown in FIG.

A flow path 821 is formed in the axial center of the cylindrical member 82 . The channel 821 is a channel through which the eluent 11A flows, and has a substantially cylindrical shape. One end of the channel 821 is connected to the channel tube insertion hole 811 . The other end of channel 821 is connected to open hole 822 . The open hole 822 is a substantially cylindrical space. The diameter of the open hole 822 is larger than the diameter of the channel 821 .

A suction filter F1A is attached to the open portion of the open hole 822 . The suction filter F1A is a thin sheet filter. In this embodiment, the suction filter F1A is a sheet-like filter formed by sintering metal fibers without using a binder. Specifically, the suction filter F1A of the present embodiment is made of a sintered metal fiber of SUS316L stainless steel. The SUS316L metal fibers are intertwined to form a thin sheet, and the SUS316L metal fibers are sintered to produce a sheet-like filter.

Metal fibers of SUS316L are, for example, very long fibers with a fiber diameter of about 100 nm to 20 μm and a fiber length of about 1 μm to 15 mm. In this embodiment, SUS316L metal fibers having a fiber diameter of about 8 μm and a fiber length of about 3 mm are used. Since SUS316L metal fibers are very long and slender fibers, the number of contact points between the fibers increases when they are entangled. A sintered body is formed without using a binder by sintering a large number of SUS316L metal fibers in an intertwined state. Thus, the suction filter F1A of this embodiment is constructed without using a binder. As the metal fiber, various metal materials or metal oxides such as silicon oxide, pure titanium, or titanium oxide can be used in addition to SUS316L.

The suction filter F1A of this embodiment is a thin sheet filter. Therefore, as shown in FIGS. 2 and 3, the space required for installing the suction filter F1A can be greatly reduced. The suction filter F1A is, for example, a circular sheet having a thickness of 0.05 mm to 2 mm and an outer diameter of about 1 mm to 25 mm. Of course, the suction filter F1A may be formed in various shapes other than circular.

In FIG. 2, the eluent 11A sucked from the bottom of the drawing passes through the suction filter F1A and flows through the open hole 822 and the flow path 821 into the flow path tube 71A. As a result, foreign matter is removed from the eluent 11A sucked from the eluent tank 1A by the suction filter F1A.

As described above, the suction filter F1A of the present embodiment is a filter provided in the flow path of the liquid chromatograph 10, and is composed of a sintered fiber material that does not use a binder. Also, the sintered body is formed in a sheet shape. Since the binder is not eluted from the suction filter F1A, it is possible to prevent the binder from flowing into the detector 6. Therefore, the flow cell of the detector 6, which has quartz in the wetted part, is prevented from being contaminated with the binder. In the chromatogram obtained from the detection result of the detector 6, occurrence of baseline drift or ghost can be prevented.

In the above embodiment, the suction filter F1A is made of a sintered metal fiber. The suction filter F1A is not limited to metal fibers, and may be made of resin fibers, glass fibers, or carbon-based materials.

Even when resin fibers are used, the suction filter F1A is composed of a sheet-like filter made by sintering resin fibers without using a binder. Examples of resin fibers include fluororesins such as PTFE (Poly Tetra Fluoro Ethylene), PCTFE (Poly Chloro Tri Fluoro Ethylene), PFA (Perfluoroalkoxy Alkane), and PEEK. Aromatic polyether ketone such as (Poly Ether Ether Ketone) or polyimide, which is a polymer having imide bonds, is used. Since the resin fibers are also very long and slender fibers, the number of points of contact between the fibers increases when they are entangled. Resin fibers are very long fibers, for example, with a fiber diameter of about 100 nm to 20 μm and a fiber length of about 1 μm to 15 mm. As a result, a sintered body is formed without using a binder by sintering a large number of resin fibers in an intertwined state.

Even when glass fibers are used, the suction filter F1A is composed of a sheet-like filter made by sintering glass fibers without using a binder. As a material used for the glass fiber, for example, quartz glass or the like is used. Since glass fibers are also very long and thin fibers, the number of points of contact between the fibers becomes large when they are entangled. Glass fibers are very long fibers, for example, with a fiber diameter of about 100 nm to 20 μm and a fiber length of about 1 μm to 15 mm. As a result, a sintered body is formed without using a binder by sintering a large number of glass fibers while being entangled with each other.

Even when carbon-based fibers are used, the suction filter F1A is composed of a sheet-like filter made by sintering carbon-based fibers without using a binder. Since carbon-based fibers are also very long and thin fibers, the number of contact points between the fibers becomes large when they are entangled. Carbon-based fibers are very long and slender fibers, for example, with a fiber diameter of about 100 nm to 20 μm and a fiber length of about 1 μm to 15 mm. As a result, a sintered body is formed without using a binder by sintering a large number of carbon-based fibers while being entangled with each other.

In the embodiment described above, the suction filter F1A has been described as an example. For the suction filter F1B, similarly, a sheet-like filter made by sintering a fiber material without using a binder can be used. Similarly, for the line filters F2A1, F2A2, F2B1, F2B2, F3A, F3B, F30, F4, F51, F52 and F6, sheet filters made by sintering fiber materials without using binders are used. be able to. That is, the suction filter F1B, the line filters F2A1, F2A2, F2B1, F2B2, F3A, F3B, F30, F4, F51, F52, and F6 are also made of metal fiber, resin fiber, glass fiber, or carbon-based fiber, and a binder is used. A sheet-like filter that has been sintered without sintering can be used. This can prevent the binder from flowing into the detector 6 in the liquid chromatograph 10 .

A filter obtained by sintering a fibrous material without using the binder of the present embodiment is formed into a thin sheet. When the filters of the present embodiment are used as the suction filters F1A and F1B, the filters are circular sheets having a thickness of, for example, 0.05 mm to 2 mm and an outer diameter of about 1 mm to 25 mm. Of course, the shape of the suction filter F1B is not limited to circular and may be configured in various shapes.

When the filters obtained by sintering the fiber material without using the binder of the present embodiment are used as the line filters F2A1, F2A2, F2B1, F2B2, F4 and F6, the thickness is, for example, 0.05 mm to 20 mm, and the outer It is composed of a circular sheet with a diameter of about 1 mm to 25 mm. Of course, the shape of the line filter is not limited to circular and may be configured in various shapes.

When the filters obtained by sintering the fiber material without using the binder of the present embodiment are used as line filters F3A, F3B and F30 for mixers, for example, the thickness is 0.1 mm to 10 mm and the outer diameter is 1 mm. It consists of a circular sheet of about 10 mm. Of course, the shape of the mixer line filter is not limited to a circle, and may be configured in various shapes.

When the filters in which the fiber material is sintered without using the binder of the present embodiment are used as the column line filters F51 and F52, the thickness is, for example, 0.05 mm to 20 mm, and the outer diameter is 1 mm to 25 mm. Consists of circular sheets of about . Of course, the shape of the column line filter is not limited to circular, and may be configured in various shapes.

In the above embodiment, the names "suction filter" and "line filter" are used, but these are names according to the application of the filter, and the structure and features of the filter used in this embodiment are the same. is.

In the present embodiment, the term "sheet-like" refers to a paper-like shape. The sheet-like filter of the present embodiment can be manufactured, for example, using the same technology (papermaking technology) as that used for manufacturing paper. A sheet of fiber material is formed by thinly spreading metal fibers, resin fibers, glass fibers, or carbon-based fibers dispersed in a liquid on a slatted surface. The sintered body of the fiber material sheet thus formed is used as the suction filter or line filter of the present embodiment. Alternatively, a laminate of sintered sheets of fiber material thus formed may be used as the suction filter or line filter of the present embodiment.

The filter obtained by sintering the fiber material without using the binder of the present embodiment may have plate-like rigidity or flexibility depending on the thickness of the sheet. .

In the above embodiment, the case where the filter formed by sintering the fibrous material without using a binder is formed into a sheet has been described as an example. A filter obtained by sintering a fibrous material without using the binder of the present embodiment may be formed in a mesh shape. For example, fibrous materials formed in a thread shape are woven as warp and weft threads on a lattice to form a mesh sheet, and the mesh sheet is sintered to form a filter. good.

In the embodiment described above, an elongated fiber material having a fiber diameter of 100 nm to 20 μm and a fiber length of 1 μm to 15 mm, for example, is used as the fiber material. Instead of using the binder of the present embodiment, the fiber material may be used as the material for the sintered filter, and chatter fibers may also be used. Chatter fibers are metal short fibers produced by direct cutting from a metal round bar by the chatter vibration cutting method. Chatter fibers are, for example, thin triangular small fibers. In addition, chatter fibers of various shapes can be used as the fiber material of the present embodiment.

(3) Experimental Results FIG. 4 shows chromatograms obtained by using the suction filters F1A and F1B of the present embodiment and chromatograms obtained by using the suction filter of the comparative example. FIG. 4(A) shows comparative example 1, FIG. 4(B) shows comparative example 2, and FIG. 4(C) shows experimental results based on the example.

4A shows a chromatogram according to Comparative Example 1. FIG. In Comparative Example 1, no filter is provided at the connecting portion between the channel pipe 71A and the eluent tank 1A and at the connecting portion between the channel pipe 71B and the eluent tank 1B. In other words, no filter is provided at a location where the eluent 11A is sucked from the eluent tank 1A and at a location where the eluent 11B is sucked from the eluent tank 1B.

4B shows a chromatogram according to Comparative Example 2. FIG. In Comparative Example 2, a sheet-like suction filter made of a sintered body using a binder was used for the connecting portion between the flow pipe 71A and the eluent tank 1A and the connecting portion between the flow pipe 71B and the eluent tank 1B. is provided. That is, a filter using a binder is provided at a location where the eluent 11A is sucked from the eluent tank 1A and at a location where the eluent 11B is sucked from the eluent tank 1B.

4(C) shows a chromatogram according to Example 1. FIG. In Example 1, a sheet-like suction filter composed of a sintered body that does not use a binder is used for the connecting portion between the flow pipe 71A and the eluent tank 1A and the connecting portion between the flow pipe 71B and the eluent tank 1B. F1A and F1B are provided. That is, it has the same configuration as the above embodiment.

The experimental conditions in FIGS. 4A to 4C are as follows. As pumps 2A and 2B, autosampler 4 and separation column 5, two pumps LC-30AD, autosampler SIL-30AC and column Shimpack VP-ODS manufactured by Shimadzu Corporation were used. The Shippack VP-ODS has a column length of 150 mm, a column diameter of 4.6 mm, and a packing material particle size of 5 μm. CTO-20AC manufactured by Shimadzu Corporation was used as a column oven for accommodating the separation column 5, and the column temperature was kept at 40°C. As the detector 6, a photodiode array detector SPD-M30A manufactured by Shimadzu Corporation was used. The measurement wavelength of SPD-M30A was set to 215 nm. A degassing unit (DGU-20A5 manufactured by Shimadzu Corporation) was provided between each of the eluent tanks 1A and 1B and the pumps 2A and 2B.

A mixture of water, acetonitrile, methanol and TFA (trifluoroacetic acid) was used as the eluent 11A. The mixing ratio is water:acetonitrile:methanol:TFA=720:196:84:1. A mixture of water, acetonitrile, methanol and TFA (trifluoroacetic acid) was used as the eluent 11B. The mixing ratio is water:acetonitrile:methanol:TFA=500:350:150:1. The mixing ratio of eluent 11A and eluent 11B was adjusted by a gradient eluent. The mixing ratio was increased to 10% after 35 minutes and 41% after 60 minutes, starting from 0% of the eluent 11B. Furthermore, the percentage of eluent 11B was increased to 100% after 70 minutes. After 70 minutes, the proportion of the eluent 11B was decreased to 0% after 95 minutes. During this period, the flow rates of the eluent 11A and the eluent 11B were each adjusted to 1.0 mL/min.

The chromatogram of the example using the suction filters F1A and F1B that do not use the binder in FIG. 4(C) is not significantly different from the chromatogram of Comparative Example 1 in which no filter is provided in FIG. 4(A). On the other hand, the chromatogram of Comparative Example 2 using the suction filter using the binder in FIG. It can be seen that there is a peak. In Comparative Example 2 of FIG. 4(B), ghost peaks are generated in the chromatogram due to the elution of the binder from the suction filter. On the other hand, in the example of FIG. 4(C), it can be seen that no ghost peak occurs in the chromatogram.

(4) Correspondence between each constituent element of the claims and each element of the embodiment An example of correspondence between each constituent element of the claim and each element of the embodiment will be described below. is not limited to In the above embodiment, the suction filters F1A and F1B and the line filters F2A1, F2A2, F2B1, F2B2, F3A, F3B, F30, F4, F51, F52 and F6 are examples of filters.

Various elements having the configuration or function described in the claims can also be used as each component of the claims.

(5) Other Embodiments As described above, glass fiber can be used as the fiber material used for the filter of this embodiment. When glass fiber is used, the filter of this embodiment is effective when used in a bio-inert analyzer. Samples such as macromolecular chemicals and proteins tend to adsorb to metals, so glass fibers are more convenient than metal fibers as the filter material. Further, since resin materials have water repellency, glass fibers are more convenient than resin fibers as materials for filters through which samples such as macromolecular chemicals and proteins pass.

Although the liquid chromatograph 10 of the above embodiment includes two eluent tanks and a mixer and performs gradient elution, this is just an example. The filter of this embodiment is of course applicable to a liquid chromatograph having one eluent tank.

In the above embodiment, the case where the suction filter F1A is attached to the suction port 8 has been described as an example, but this is only an example. The sheet-like filter of this embodiment can be attached to various members.

The specific configuration of the present invention is not limited to the above-described embodiment, and various changes and modifications are possible without departing from the gist of the invention.

(6) Aspects It will be appreciated by those skilled in the art that the exemplary embodiments described above are specific examples of the following aspects.

(Section 1) The filter used in the liquid chromatograph according to one aspect is
A filter provided in a channel of a liquid chromatograph,
The filter is composed of a sintered body of a fibrous material that does not use a binder, and the sintered body is formed into a sheet.

A filter used in this liquid chromatograph is constructed without using a binder. In a liquid chromatograph, it is possible to prevent the binder from flowing into the detector.

(Section 2) A filter used in the liquid chromatograph according to Section 1, wherein the fiber material may include a metal material.

A filter is constructed by sintering a metal material without using a binder. In a liquid chromatograph, it is possible to prevent the binder from flowing into the detector.

(Item 3) A filter used in the liquid chromatograph according to Item 1, wherein the fiber material may include a resin material.

A filter is constructed by sintering a resin material without using a binder. In a liquid chromatograph, it is possible to prevent the binder from flowing into the detector.

(Item 4) A filter used in the liquid chromatograph according to Item 1, wherein the fiber material may include a glass material.

A filter is constructed by sintering a glass material without using a binder. In a liquid chromatograph, it is possible to prevent the binder from flowing into the detector. Since a glass material is used, it is also convenient when used for analysis of pharmaceuticals, proteins, or the like.

(Item 5) A filter used in the liquid chromatograph according to Item 1, wherein the fiber material may include a carbonaceous material.

(Item 6) The filter used in the liquid chromatograph according to any one of items 1 to 5, wherein the filter may have a thickness of 0.05 mm to 20 mm.

Since the filter used in this liquid chromatograph has a thin sheet shape, the filter can be attached even in a narrow space.

(Item 7) A filter used in the liquid chromatograph according to any one of items 1 to 5, wherein the filter sucks the eluent from the eluent tank of the liquid chromatograph , pumps, mixers, autosamplers, separation columns or detectors.

(Section 8) A liquid chromatograph according to one aspect includes the filter according to any one of the first to fifth items.

Claims (9)

a detector having a flow cell;
a filter provided in the channel of the liquid chromatograph;
with
The filter is composed of a sintered body of a fibrous material that does not use a binder, and the sintered body is formed in a sheet shape in which the fibrous material is entangled, so that the binder flows into the flow cell and contaminates it. A liquid chromatograph configured to prevent 2. The liquid chromatograph of claim 1, wherein said fibrous material comprises metallic material. 2. The liquid chromatograph according to claim 1, wherein said fibrous material comprises a resin material. 2. The liquid chromatograph of claim 1, wherein said fibrous material comprises a glass material. 2. The liquid chromatograph according to claim 1, wherein said fibrous material comprises a carbonaceous material. The liquid chromatograph according to any one of claims 1 to 5, wherein the filter has a thickness of 0.05 mm to 20 mm. a detector having a flow cell;
a filter provided in the channel of the liquid chromatograph;
with
The filter is composed of a sintered body of a fibrous material that does not use a binder, and the sintered body is formed in a sheet shape so as to prevent the flow cell from being contaminated by the flow of the binder. ,
The liquid chromatograph, wherein the fiber material has a fiber diameter of 100 nm to 20 μm and a fiber length of 1 μm to 15 mm. The flow cell is a quartz flow cell, the filter is composed of a sintered body of a fibrous material that does not use a binder, and the sintered body is formed into a sheet so that the binder is attached to the quartz flow cell. 8. The liquid chromatograph according to any one of claims 1 to 7, configured to prevent inflow and contamination. Claims 1 to 8, wherein the filter is used in a portion for sucking an eluent from an eluent tank of a liquid chromatograph, a pump, a mixer, an autosampler, a separation column or a detector.
The liquid chromatograph according to any one of .

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