JP7215726B2 - ACTIVE SPECIES-CONTAINING LIQUID INJECTION DEVICE AND STERILIZATION AND EXTERMINATION METHOD - Google Patents

Description

TECHNICAL FIELD The present invention relates to an active species-containing liquid injection device and a sterilization and extermination method.

Conventionally, as a device for sterilizing and exterminating pathogens and insect pests using plasma, a reaction vessel, an injection port communicating with the inside of the reaction vessel, a liquid supply unit for supplying liquid to the inside of the reaction vessel, and plasma. and a plasma generating means configured to generate plasma in the vicinity of the liquid supplied to the reaction vessel, wherein the liquid and the liquid are inside the reaction vessel. When the gas is supplied, the plasma generating means generates plasma in the vicinity of the liquid to generate active species of the gas and/or the liquid, and the active species is configured to inject the liquid containing the active species from the injection port. A seed-containing liquid injection device has been developed by the present inventors (see, for example, Non-Patent Document 1).

In addition, as a method of sterilizing medical instruments, living bodies, etc. using plasma, active species generated by plasma are brought into contact with a liquid, and the active species are diffused in the liquid to generate a plasma-treated liquid. There is a method of applying a plasma-treated liquid to an object (see, for example, Patent Document 1). In this method, the generated plasma-treated liquid is stored at a temperature of 10° C. or lower or frozen so that the active species diffused in the plasma-treated liquid are not lost as much as possible.

Kenji Nihei, Keisuke Takashima, Toshiro Kaneko, "Generation of short-lived active species and inhibition of conidial germination of strawberry anthracnose fungus by plasma irradiation solution direct spray device", The 65th Japan Society of Applied Physics Spring Conference Proceedings, 2018 , 19p-C201-17

International publication WO2013/161327

The device described in Non-Patent Document 1 generates active species of a supplied gas or liquid in a reaction vessel, and by incorporating the active species into the liquid, the sterilization effect is greater than when it exists in the gas. can be increased, and an excellent bactericidal effect can be obtained. However, since the temperature of the liquid containing the active species rises due to the heat generated when the plasma is generated in the reactor, if the plasma discharge time is extended, there is a risk that the short-lived active species will be deactivated. There is a problem that the sterilization effect may decrease.

In addition, in the method described in Patent Document 1, active species diffused in the plasma-treated liquid are prevented from disappearing by storing the plasma-treated liquid at a temperature of 10° C. or lower or by freezing it. Another problem is that spraying a cold plasma-treated liquid or an ice-like plasma-treated liquid on a plant, a living body, or the like poses a high risk of damaging them.

The present invention has been made by paying attention to such problems, and can maintain a high sterilization effect even if the plasma discharge time is extended, and can safely sterilize plants and the like. An object of the present invention is to provide an active species-containing liquid injection device and a sterilization and extermination method.

In order to achieve the above object, an active species-containing liquid injection device according to the present invention is provided so as to generate plasma using a gas that becomes plasma, and to generate a plasma active gas containing the active species of the gas. a plasma generating portion, a cooling means provided to cool the generated plasma active gas, and a solution containing the active species by dissolving the plasma active gas cooled by the cooling means in a liquid. and a dissolution injection means provided to inject the solution, the dissolution injection means being a dissolution vessel provided to introduce the plasma active gas cooled by the cooling means into the interior thereof; A liquid supply unit that supplies the liquid to the interior of the dissolution vessel and communicates with the interior of the dissolution vessel so that the plasma-activated gas and the liquid come into contact with each other inside the dissolution vessel to generate the dissolution liquid. and an injection port provided to inject the solution generated inside the dissolution container, and the liquid supply unit is a cylinder inserted into the dissolution container and through which the liquid flows. A cylindrical tube is characterized in that it has one or more holes in its side surface .

The active species-containing liquid injection device according to the present invention dissolves a plasma active gas containing gas active species in a liquid to generate a solution containing gas active species, thereby producing peroxynitrite in the solution. By generating active species with high sterilizing ability such as (HOONO) and injecting the solution thereof onto the object, the object can be sterilized. In the active species-containing liquid injection device according to the present invention, the plasma active gas generated in the plasma generating section is cooled by the cooling means and then dissolved in the liquid to generate the dissolved liquid, so that the temperature of the dissolved liquid is suppressed from rising. can do. Further, even when the plasma discharge time in the plasma generating section is extended, the plasma generating gas is cooled by the cooling means, so that the temperature rise of the solution can be suppressed. Therefore, it is possible to prevent deactivation of the short-lived active species in the solution due to temperature rise, and maintain a high sterilization effect.

In addition, the active species-containing liquid injection device according to the present invention does not store the solution, and the plasma active gas can be cooled by the cooling means to room temperature or about the outside temperature. can be sprayed, and sterilization and the like can be safely performed on plants and the like. Since the active-species-containing liquid injection device according to the present invention sprays the solution, it is difficult to spread due to wind or the like compared to the case of injecting gas. Therefore, even when used for outdoor plants, etc., a high bactericidal effect can be maintained. In addition, by injecting the solution containing active species immediately after generation, even active species with a short life can be attached to the object before they are deactivated. In addition, since the sterilization effect is enhanced when the active species are present in the liquid rather than in the gas, a more excellent sterilization effect can be obtained as compared with the case where the plasma active gas is directly injected.

INDUSTRIAL APPLICABILITY The active species-containing liquid injection device according to the present invention can be used, for example, in the field of agriculture to sterilize and exterminate pathogens and insect pests adhering to plants, soil, fertilizers, and the like. Pathogens that are sterilized or exterminated are mainly organisms that cause plant diseases, such as pathogens such as filamentous fungi (mainly molds) and bacteria (bacteria), viruses, and the like. Pathogenic fungi include, for example, rice blast, wheat powdery mildew, soybean purpura, strawberry gray mold, cucumber gray mold, tomato gray mold, lily leaf blight, cucumber powdery mildew, strawberry powdery mildew, tomato leaf These include mold, leek rust, chrysanthemum white rust, leek black spot, apple spot leaf spot, cucumber brown spot, chrysanthemum anthracnose, leaf blight of Japanese parsley, apple brown spot, and bakanae disease. Pests are pests that primarily damage plants, such as mites and aphids.

In the active species-containing liquid injection device according to the present invention, the gas that becomes plasma may be any gas such as air that can generate plasma. Further, it is preferable that the cooling means is configured to cool the plasma active gas by air cooling and/or water cooling while the plasma active gas moves from the plasma generating portion to the dissolution injection means. Further, the liquid that dissolves the plasma active gas is preferably a liquid such as water that can dissolve the active species of the gas and generate active species in the solution such as nitrous oxide (HOONO).

In the active species-containing liquid injection apparatus according to the present invention, the dissolving and injecting means includes a dissolving container provided to introduce the plasma active gas cooled by the cooling means into the interior, and the plasma in the dissolving container. a liquid supply unit that supplies the liquid to the interior of the dissolution vessel so that the active gas and the liquid come into contact with each other to generate the dissolution liquid; Since the injection port is provided so as to inject the dissolved solution, the dissolved solution can be easily generated and injected by the dissolution injection means.

Further, in the active species-containing liquid injection device according to the present invention, the liquid supply unit has a cylindrical tube inserted into the dissolution container through which the liquid flows, and the cylindrical tube has one side surface. Alternatively, since it has a plurality of holes, the plasma-activated gas introduced into the dissolution container is brought into contact with the liquid flowing through the cylindrical tube through the holes on the side surface of the cylindrical tube to generate a dissolved liquid. can be done. The cylindrical tube may, for example, be coiled, have a net-like, mesh-like, or grid-like side surface, or may have one or more slits in the side surface so as to increase the chances of contact between the liquid and the plasma-activated gas. may have

Further, when the cylindrical tube is provided, the liquid supply section is provided so as to be able to supply the liquid to the inside of the dissolution container at a predetermined pressure higher than 1 atm, and the plasma generation section generates It is preferable that the plasma active gas be supplied to the interior of the dissolution vessel at a pressure higher than the predetermined pressure, and that the cylindrical tube be provided to communicate with the injection port. At this time, the pressure of the plasma active gas can prevent the liquid flowing through the tubular tube from leaking from the side holes, and the plasma active gas is forced through the tubular tube through the side holes of the tubular tube. It can be permeated by flowing liquids. Thereby, the solution can be efficiently generated. In addition, the pressure of the liquid flowing through the cylindrical tube can force the generated dissolution liquid to be ejected from the ejection port.

A sterilization and extermination method according to the present invention is a sterilization and extermination method using the active species-containing liquid injection device according to the present invention, wherein plasma is generated using a gas that becomes plasma, and the active species of the gas is included. A plasma active gas is generated, the generated plasma active gas is cooled, the plasma active gas is dissolved in a liquid to generate a solution containing the active species, and the generated solution is sprayed onto an object. to sterilize or exterminate pathogenic bacteria, viruses or pests adhering to the object.

The sterilization extermination method according to the present invention can be suitably carried out by the active species-containing liquid injection device according to the present invention. The sterilization and extermination method according to the present invention can suppress the temperature rise of the solution even when the plasma discharge time is extended, so that the short-lived active species in the solution can be prevented from being deactivated due to the temperature rise. It is possible to maintain a high sterilization effect. In addition, in the sterilization and extermination method according to the present invention, by cooling the plasma active gas to room temperature or about the outside temperature, it is possible to spray the solution at an appropriate temperature to the plants, etc., and it is safe for the plants. sterilization etc. can be performed.

According to the present invention, there is provided an active species-containing liquid injection device and a sterilization and extermination method that can maintain a high sterilization effect even if the plasma discharge time is extended, and can safely sterilize plants and the like. can provide.

BRIEF DESCRIPTION OF THE DRAWINGS It is a whole block diagram which shows the active-species containing liquid injection apparatus of embodiment of this invention. 2 is a graph showing the relationship between the discharge time in the plasma generating part and the germination rate of Colletotrichum gloeosporioides (C.glo) conidia, which is a kind of strawberry anthracnose fungus, in a sterilization test using the active species-containing liquid injection device shown in FIG. . In the sterilization test using the active species-containing liquid injection device shown in FIG. It is a graph showing. Fig. 2 is a graph showing the relationship between the injection distance and the germination rate of C.glo conidia in a sterilization test using the active species-containing liquid injection device shown in Fig. 1; Changes in the germination rate of C.glo conidia in the sterilization test using the active species-containing liquid injection device shown in FIG. is a graph showing (a) Discharge time and discharge time in the plasma generation part in a sterilization test using the active species-containing liquid injection device shown in FIG. 1 and the device using an external mixing nozzle that mixes the plasma active gas and liquid outside the injection port Graph showing the relationship with the germination rate of C. glo conidia, (b) A longitudinal sectional view showing the external mixing nozzle used in (a).

BEST MODE FOR CARRYING OUT THE INVENTION An embodiment of the present invention will be described below based on the drawings.
1 to 6 show an active species-containing liquid injection device and a sterilization and extermination method according to an embodiment of the present invention.
As shown in FIG. 1, the active species-containing liquid injection device 10 has a plasma generating section 11, a cooling means 12, and a dissolution injection means 13. As shown in FIG.

The plasma generation section 11 has a reaction vessel 21 , a gas supply section 22 , a pair of electrodes 23 a and 23 b and a power supply section 24 . The reaction vessel 21 consists of an elongated tubular quartz tube. The gas supply unit 22 is composed of a gas cylinder filled with a plasma gas, and is connected to an opening at one end of the reaction vessel 21 . The gas supply unit 22 can supply gas to the inside of the reaction vessel 21 at a predetermined pressure. In the specific example shown in FIG. 1, the gas consists of air, but it may be nitrogen, oxygen, helium, argon, or any other gas capable of generating plasma.

One electrode 23 a is inserted into the reaction container 21 and arranged to extend along the length direction of the reaction container 21 . The other electrode 23b is made of copper foil or the like and has a thin sheet shape. The other electrode 23b is attached by wrapping around the outer surface of the reaction container 21 over a predetermined range in the vertical direction of the reaction container 21 so as to face the one electrode 23a. Also, the other electrode 23b is grounded. The power supply unit 24 is connected to one electrode 23a and can apply a voltage between the electrodes 23a and 23b. In the specific example shown in FIG. 1, the power supply unit 24 is an AC power supply, but may be a DC power supply or a pulse power supply.

The plasma generator 11 applies a voltage between the electrodes 23a and 23b from the power source 24 in a state in which a gas to be plasma is supplied from the gas supply unit 22 to the inside of the reaction container 21, whereby the reaction container 21 is Plasma can be generated inside. Thereby, the plasma generation unit 11 can generate a plasma active gas containing gas active species. The plasma generation unit 11 can discharge the generated plasma active gas from the opening of the other end of the reaction vessel 21 by the pressure of the gas from the gas supply unit 22 and supply it to the dissolution injection means 13 . .

The cooling means 12 has an air cooling fan 25 and a cooling circuit 26 . The cooling means 12 is provided in the middle of the gas flow path 14 for flowing the plasma active gas from the plasma generating section 11 to the dissolution injection means 13 so as to cool the moving plasma active gas. The air-cooling fan 25 is provided to blow air toward the gas flow path 14 and is configured to cool the plasma active gas in an air-cooling manner. The cooling circuit 26 is provided so as to surround the gas flow path 14 and is configured to cool the plasma active gas by water cooling.

The dissolution injection means 13 has a dissolution container 27 , a liquid supply section 28 and an injection port 29 . The melting container 27 has an elongated cylindrical shape, and is provided so that the plasma active gas cooled by the cooling means 12 can be introduced into the inside through an opening on one end side.

The liquid supply section 28 has a liquid pump 31 and a tubular tube 32 . The liquid pump 31 is connected to an opening on one end side of the dissolving container 27 and is capable of supplying liquid such as water into the dissolving container 27 at a predetermined pressure. A tubular tube 32 is inserted inside the dissolution vessel 27 and connected to a liquid pump to flow the liquid supplied by the liquid pump 31 . The cylindrical tube 32 has a coil shape and has a plurality of holes on its side surface. The liquid supply unit 28 brings the plasma active gas into contact with the liquid flowing through the tubular tube 32 through the hole in the side surface of the tubular tube 32 inside the dissolution vessel 27 to produce a dissolved liquid containing gas active species. It is designed to generate In the specific example shown in FIG. 1, the liquid supplied by the liquid pump 31 is water. Any material can be used as long as it can generate an active species.

The injection port 29 is provided at an opening on the other end side of the dissolving container 27 and communicates with the tubular tube 32 inside the dissolving container 27 . The dissolution injection means 13 injects the solution generated inside the dissolution container 27 from the injection port 29 by the pressure of the plasma active gas or the pressure of the liquid from the liquid pump 31 .

The sterilization and extermination method of the embodiment of the present invention can be suitably carried out by the active species-containing liquid injection device 10. That is, in the sterilization and extermination method according to the embodiment of the present invention, plasma is generated by the plasma generator 11 using gas that becomes plasma, and plasma active gas containing active species of the gas is generated. Next, after the generated plasma active gas is cooled by the cooling means 12, the plasma active gas is dissolved in a liquid by the dissolution injection means 13 to generate a solution containing active species, and the generated solution is used as a target. spray on things. As a result, it is possible to sterilize or exterminate pathogenic bacteria, viruses, or pests adhering to the object.

In the activated species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention, the plasma activated gas containing the gas activated species is dissolved in the liquid to generate the solution containing the gas activated species, An object can be sterilized by generating active species with high sterilizing ability such as peroxynitrite (HOONO) in the solution and injecting the solution to the object. In the active species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention, after cooling the plasma active gas generated in the plasma generation unit 11 by the cooling means 12, it is dissolved in the liquid to generate the solution. , it is possible to suppress the temperature rise of the solution. Further, even when the plasma discharge time in the plasma generating section 11 is extended, the plasma generating gas is cooled by the cooling means 12, so that the temperature rise of the solution can be suppressed. Therefore, it is possible to prevent deactivation of the short-lived active species in the solution due to temperature rise, and maintain a high sterilization effect.

In addition, the activated species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention do not store the dissolved liquid, and the plasma active gas may be cooled to room temperature or about the outside temperature by the cooling means 12. , it is possible to spray the solution at an appropriate temperature onto the plants or the like, and to safely perform sterilization or the like on the plants or the like. Since the active species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention spray the solution, it is difficult to spread due to wind or the like compared to the case of injecting gas. Therefore, even when used for outdoor plants, etc., a high bactericidal effect can be maintained. In addition, by injecting the solution containing active species immediately after generation, even active species with a short life can be attached to the object before they are deactivated. In addition, since the sterilization effect is enhanced when the active species are present in the liquid rather than in the gas, a more excellent sterilization effect can be obtained as compared with the case where the plasma active gas is directly injected.

The active species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention can be used, for example, in the agricultural field to sterilize and exterminate pathogens and pests adhering to plants, soil, fertilizers, etc. can. Pathogens that are sterilized or exterminated are mainly organisms that cause plant diseases, such as pathogens such as filamentous fungi (mainly molds) and bacteria (bacteria), viruses, and the like. Pathogenic fungi include, for example, rice blast, wheat powdery mildew, soybean purpura, strawberry gray mold, cucumber gray mold, tomato gray mold, lily leaf blight, cucumber powdery mildew, strawberry powdery mildew, tomato leaf These include mold, leek rust, chrysanthemum white rust, leek black spot, apple spot leaf spot, cucumber brown spot, chrysanthemum anthracnose, leaf blight of Japanese parsley, apple brown spot, and bakanae disease. Pests are pests that primarily damage plants, such as mites and aphids.

In addition, in the active species-containing liquid injection device 10 and the sterilization and extermination method of the embodiment of the present invention, the liquid supply unit 28 is provided so as to be able to supply the liquid into the dissolution container 27 at a predetermined pressure higher than 1 atm. The plasma generating section 11 may be provided so as to be able to supply the generated plasma active gas into the melting vessel 27 at a pressure higher than a predetermined pressure. At this time, the pressure of the plasma active gas can prevent the liquid flowing through the tubular tube 32 from leaking from the hole in the side surface of the tubular tube 32, and the plasma active gas is forced through the hole in the side surface of the tubular tube 32 into the tubular shape. It can be permeated by liquid flowing through the tube 32 . Thereby, the solution can be efficiently generated. In addition, the pressure of the liquid flowing through the tubular tube 32 allows the generated solution to be vigorously jetted from the jet port 29 .

A sterilization test was conducted on Colletotrichum gloeosporioides (C.glo), which is a kind of strawberry anthracnose fungus, using the active species-containing liquid injection device 10 shown in FIG. Since C.glo conidia germinate in water, in the test, a dissolution solution containing active species was sprayed toward the suspension in a petri dish containing pre-germinated C.glo conidia, and after a predetermined period of time, The germination status of C.glo conidia was investigated.

In the test, a suspension of pre-germinated C.glo conidia in distilled water was used at a concentration of 1.0×10 ⁷ conidia/mL. Also, the voltage applied to the power supply unit 24 of the plasma generation unit 11 was set to 15.5 kV, and the frequency was set to 8 to 8.1 kHz. Below, the amount of gas (air) supplied from the gas supply unit 22, the amount of liquid (water) supplied from the liquid pump 31, the injection time of the solution containing the active species, the injection port 29 and the surface of the suspension The test was conducted by changing the distance (injection distance) and the amount of suspension, etc., and the germination state of C. glo conidia 30 minutes after the injection was stopped was observed.

First, the discharge between the electrodes 23a and 23b in the plasma generating section 11 was continued, and the solution was injected every predetermined elapsed time (discharge time) from the start of the discharge to measure the germination rate. In this test, the air supply rate was 4 L/min, the water supply rate was 11 mL/min, the solution injection time was 10 seconds, the injection distance was 10 cm, and the suspension amount was 50 μL. As test results, the relationship between discharge time and germination rate is shown in FIG.

As shown in FIG. 2, even if the discharge was continued for one hour, the germination rate remained at a small value of 10% to several % or less, and was almost constant, confirming that the sterilization effect was obtained. From this, it can be said that a high sterilization effect is maintained even if the plasma discharge time is extended.

Next, the germination rate was measured when the amount of suspension was 25 μL, 50 μL, and 100 μL, and the injection time of the solution was set to 5 seconds, 10 seconds, and 20 seconds. In this test, the air supply rate was 4 L/min, the water supply rate was 11 mL/min, and the injection distance was 10 cm. The test results are shown in FIG. FIG. 3 also shows the measurement result (w/o plasma) when a mixture of water and air is injected for 10 seconds without plasma.

As shown in FIG. 3, it was confirmed that when the amount of suspension was as small as 25 μL and 50 μL, a sterilization effect was obtained even with an injection time of 5 seconds or 10 seconds. Moreover, it was confirmed that when the amount of the suspension was as large as 100 μL, the sterilization effect was obtained by extending the injection time to 20 seconds. From this result, it can be said that the sterilization ability is maintained even if the ejection time of the solution is extended, and that the longer the ejection time is, the higher the sterilization effect is.

Next, the germination rate was measured when the injection distance was 10 cm, 15 cm, and 20 cm. At this time, for each injection distance, the injection time was changed so that the injection amount of the dissolution liquid in a petri dish with a diameter of 1.5 cm was constant at 0.85 mL. Specifically, the ejection time of the solution is 10 seconds when the ejection distance is 10 cm, 18 seconds when the ejection distance is 15 cm, and 25 seconds when the ejection distance is 20 cm. In this test, the air supply rate was 4 L/min, the water supply rate was 11 mL/min, and the suspension amount was 50 μL. The test results are shown in FIG. FIG. 4 also shows the measurement results (w/o plasma) when a mixture of water and air is injected for 10 seconds without plasma.

As shown in FIG. 4, it was confirmed that the same degree of sterilization effect can be obtained if the injection amount of the solution is the same even if the injection distance changes. From this, it is considered that the active species in the solution are not deactivated while the solution is scattered from the injection port 29 to the suspension. From this result, when sterilizing or exterminating a plant, by injecting the solution from a predetermined height, even a leaf near the injection port 29 (a leaf at a high position) can be removed. It can be said that the same degree of effect can be obtained by adjusting the injection time even in the case of the leaves of .

Next, the germination rate was measured by changing the amount of air supplied and the amount of water supplied. In this test, the injection time of the solution was 10 seconds, the injection distance was 10 cm, and the amount of suspension was 50 μL. FIG. 5(a) shows the test results when the air supply rate is changed (water supply rate is 11 mL/min) and when the water supply rate is changed (air supply rate is 4 L/min). and (b).

As shown in FIG. 5(a), it was confirmed that the sterilization effect decreased as the amount of air supplied increased. Moreover, it was confirmed that the germination rate was 5% or less when the supply amount was about 4 L/min or less, and a very high bactericidal effect was obtained. As shown in FIG. 5(b), it was confirmed that the sterilization effect was enhanced by increasing the amount of water supplied. Moreover, it was confirmed that when the supply amount was about 3.3 mL/min or more, the germination rate was 5% or less, and a very high bactericidal effect was obtained.

Next, the active species containing liquid injection device 10 that mixes the plasma active gas and the liquid inside the dissolution container 27 and injects it from the injection port 29, and the plasma active gas and the liquid instead of the dissolution injection means 13 A device using an external mixing nozzle for mixing outside the injection port was tested for germination inhibition effect. In the test, discharge was continued in the plasma generating part 11, and the germination rate was measured every predetermined discharge time. In the test, the air supply rate was 4 L/min, the water supply rate was 11 mL/min, the solution injection time was 10 seconds, the injection distance was 10 cm, and the suspension amount was 50 μL. The test results are shown in FIG. 6(a).

Note that the dissolution injection means 13 of the active species-containing liquid injection device 10 supplies the plasma active gas to the dissolution container 27 at a pressure higher than 1 atm, dissolves it in the liquid from the liquid supply part 28, and generates a solution, It is injected from the injection port 29 and is hereinafter referred to as an internal mixing nozzle. Further, as shown in FIG. 6B, the used external mixing nozzle 40 injects the liquid as it is from the injection port 41, and the plasma active gas from the gas injection port 42 provided around the injection port 41. The liquid and the plasma active gas are mixed under the atmospheric pressure outside the injection port 41 by jetting toward the liquid.

As shown in FIG. 6(a), even if the internal mixing nozzle continues discharging for 1 hour, the germination rate is almost constant at a small value of 10% to several % or less, indicating that the sterilization effect is obtained. was confirmed. On the other hand, the external mixing nozzle 40 has a high germination rate of about 70% or more regardless of the discharge time, confirming that the sterilization effect is hardly obtained. From this, it can be said that by mixing the liquid and the plasma-activated gas under high pressure, an excellent germination suppressing effect can be obtained. The test results of the internal mixing nozzle shown in FIG. 6(a) are the same as the test results shown in FIG.

REFERENCE SIGNS LIST 10 Active species-containing liquid injection device 11 Plasma generation unit 21 Reaction vessel 22 Gas supply unit 23a, 23b Electrode 24 Power supply unit 12 Cooling means 25 Air cooling fan 26 Cooling circuit 13 Dissolution injection means 27 Dissolution container 28 Liquid supply unit 31 Liquid pump 32 Cylinder shaped tube 29 injection port 14 gas flow path

40 External Mixing Nozzle 41 Jet Orifice 42 Gas Jet Orifice

Claims (4)

a plasma generation unit capable of generating plasma by using a gas to be plasma and generating a plasma active gas containing active species of the gas;
a cooling means capable of cooling the generated plasma active gas;
dissolving and injecting means for dissolving the plasma active gas cooled by the cooling means into a liquid to generate a solution containing the active species, and for injecting the solution ;
The dissolution injection means includes a dissolution container provided to introduce the plasma active gas cooled by the cooling means into the inside, and the plasma active gas and the liquid come into contact with each other inside the dissolution container to cause the dissolution. a liquid supply unit for supplying the liquid to the inside of the dissolution container so as to generate a liquid; The liquid supply part has a cylindrical tube inserted into the dissolution container and through which the liquid flows, and the cylindrical tube has one or more holes on its side surface. have
An active species-containing liquid injection device characterized by: 3. The cooling means is configured to cool the plasma active gas by air cooling and/or water cooling while the plasma active gas moves from the plasma generating portion to the dissolution injection means. 2. The active species-containing liquid injection device according to 1. The liquid supply unit is provided so as to be able to supply the liquid to the inside of the dissolution container at a predetermined pressure higher than 1 atmosphere,
The plasma generation unit is provided so as to be able to supply the generated plasma active gas to the interior of the melting vessel at a pressure higher than the predetermined pressure,
3. The active species-containing liquid injection device according to claim 1, wherein the cylindrical pipe is provided so as to communicate with the injection port. A sterilization and extermination method using the active species-containing liquid injection device according to any one of claims 1 to 3,
generating a plasma using a plasma-forming gas to generate a plasma active gas containing active species of the gas;
After cooling the generated plasma active gas, dissolving the plasma active gas in a liquid to produce a solution containing the active species,
A sterilization and extermination method, comprising spraying the generated dissolution liquid onto an object to sterilize or exterminate pathogenic bacteria, viruses, or pests adhering to the object.

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