JP7216486B2 - Yeast extract with skin moisturizing effect - Google Patents

Description

The present invention provides a yeast extract that exerts a moisturizing effect and an effect of reducing wrinkles and color unevenness on human skin by oral ingestion. The present invention also provides a supplement containing the yeast extract as an active ingredient.

It has long been desired by many people to keep the skin beautiful against deterioration of skin symptoms such as dryness, spots, wrinkles, and sagging caused by factors such as aging, stress, and exposure to ultraviolet rays. For this reason, various studies are still being conducted to find means for preventing or improving the deterioration of skin symptoms. Polyamine is known as one of the ingredients that are suggested to prevent or improve the deterioration of these skin symptoms and contribute greatly to beauty.

Polyamines (putrescine (PUT), spermidine (SPD), spermine (SPM)) are present in the cells of many organisms, including human cells, and mainly interact with RNA to promote the synthesis of proteins and nucleic acids. It is said to be a growth factor (Non-Patent Document 1). In fact, the cell activating effect of plant-derived polyamine-containing extracts has been confirmed in human skin fibroblasts, and the activating effect has been accompanied by collagen production promoting effects and hyaluronic acid production promoting effects (Patent Document 1). ).

Nucleotides, like polyamines, are also widely present in living organisms and are substances that play a fundamental role in life activities. Currently, studies have reported that dietary nucleotides restore the intestinal morphology (villus height, crypt depth, and intestinal wall thickness) in mice with damaged intestinal mucosa. The importance of sex nucleotides has been suggested (Non-Patent Document 2).

However, it has not been known that yeast extracts containing nucleotides and polyamines are cosmetically effective.

JP 2012-46544 A

Igarashi k, Kashiwagi k. Polyamines: Mysterious Modulators of Cellular Functions. Biochemical and Biophysical Research Communications 2000 ; 271 : 559-564 Adjei AA, Ohshiro Y, Yamaguchi K, Nakasone Y, Shimada K, Iwanaga M, et al. Intraperitoneal administration of nucleoside-nucleotide mixture inhibits endotoxin-induced bacterial translocation in mice fed protein-deficient diet. Tohoku J. Exp. Med. 1994 ; 174 : 1

An object of the present invention is to provide a yeast extract that, when orally ingested, exerts a moisturizing effect on human skin and an effect of reducing wrinkles and color unevenness. Another object of the present invention is to provide a supplement containing the yeast extract as an active ingredient.

In the present invention, it was found that ingestion of a yeast extract containing nucleotides and polyamines exerts a moisturizing effect on human skin.

The skin-moisturizing yeast extract containing nucleotides and polyamines of the present invention can be orally ingested because it is a general food, and has a moisturizing effect that increases the moisture content of the stratum corneum and an effect that reduces wrinkles and uneven color. Therefore, it can be effectively used for beauty purposes.

The present invention will be described in detail below.
The yeast extract for skin moisturizing or for reducing wrinkles and uneven coloring of the present invention is not particularly limited as long as it contains nucleotides and polyamines, but nucleotides are guanylic acid, adenylic acid, cytidylic acid, uridylic acid and nucleotides thereof Yeast extracts containing salts such as sodium salts and potassium salts are preferred.
_The yeast extract is not particularly limited as long as it contains these compounds. %, preferably 3-15% by weight each.

Polyamine in the present invention refers to spermine, spermidine, putrescine, or cadaverine, and the yeast extract of the present invention contains one or more of them. In particular, it preferably contains three polyamines, spermine, spermidine, and putrescine. The total content of polyamines in the yeast extract is preferably 0.1% by weight or more, more preferably 0.2% by weight or more. The composition ratio of the polyamine is particularly preferably 10 to 90% by weight of spermine, 10 to 90% of spermidine, 0 to 90% of putrescine, and/or 0 to 20% of cadaverine.

The yeast extract of the present invention can be prepared by a known method, for example, a method of extracting yeast with hot water of pH 5 to 12, a method of extracting yeast by adding a proteolytic enzyme, a cell wall degrading enzyme, or the like, and a high pressure such as high pressure dispersion treatment. Among these, yeast extract obtained by extracting yeast with hot water, and yeast extract obtained by allowing a nucleolytic enzyme to act on the extracted yeast extract are the nucleotides and high polyamine content. In addition, commonly available yeast extracts include, for example, the yeast extract “Nucleamine (registered trademark)” manufactured by Kohjin Life Sciences.

The yeast extract may be liquid or powdery, but powdery is preferred. A powdery yeast extract can be easily produced from an extract by a conventional method such as concentration or spray drying.

As the yeast used for producing the yeast extract of the present invention, any commonly used yeast can be used. Specific examples include baker's yeast, brewer's yeast (Saccharomyces cerevisiae), torula yeast (Candida utilis), and the like. Among these, torula yeast is particularly desirable.

The yeast extract of the present invention can be ingested as it is, or mixed with other materials and ingested as foods, beverages, luxury goods, supplements, pharmaceuticals, and the like. The form is not particularly limited. Moreover, the forms of supplements and pharmaceuticals are not particularly limited, and tablets, capsules, soft capsules, and nutritional drinks may be used.

The amount of the yeast extract in the supplement is not particularly limited as long as it has a skin moisturizing effect. It is more desirable to blend to ~500 mg.

EXAMPLES The present invention will be described in more detail with reference to examples below, but the present invention is not limited to these examples.

(test food)
Nucleamine (registered trademark) (manufactured by Kohjin Life Sciences Co., Ltd.) was used as the nucleotide- and polyamine-containing torula yeast extract. The yeast extract "Nucleamine (registered trademark)" used this time contains 10.1% adenylic acid, 10.5% guanylic acid, 7.6% cytidylic acid and uridylic acid per 100 weight g of yeast extract. contained 8.6% each (all as 2Na.7H ₂ O products). Also, spermidine, spermine and putrescine were contained in amounts of 200 mg, 98 mg and 65 mg, respectively, per 100 g of yeast extract weight.
As test foods, a hard capsule containing 60 mg of yeast extract Nucleamine (registered trademark) and 340 mg of dextrin (hereinafter referred to as test food) and a hard capsule containing 400 mg of dextrin (hereinafter referred to as placebo) were used.

(Test design and intake method)
The study was conducted as a randomized, double-blind, parallel-group study. For 30 women aged 20 to 60 (average age 44.6 ± 8.0 years old), "keratin moisture content" at the time of the following <preliminary examination> was used as the main stratification factor, and clinical examination "T-CHO" was performed. After allocating into 2 groups (15 people/group) by stratification considering the value of "LDL-CHO", the test food was randomly assigned, and it was confirmed that there was no significant difference between the test food groups. bottom. Five tablets of the test food were ingested daily after dinner.

(Inspection item)
<Preliminary inspection>
Selected study participants were tested within 4 weeks prior to the study. In the examination, keratin moisture content and VISIA (wrinkles, uneven color) were measured.
<Main test (4 weeks, 8 weeks later)>
In this test, the test was conducted twice after 4 weeks and 8 weeks after the start of intake of the test food. In the examination, keratin moisture content and VISIA (wrinkles, uneven color) were measured.

(Statistical analysis)
All data are shown as mean ± standard deviation. Paired t-tests were used for before and after group comparisons, corrected with Bonferroni. The statistical significance level was set at 5%. An unpaired t-test was used to compare the amount of change between the placebo group and the test diet group. The statistical significance level was set at 5%.

Table 1 shows the results of the stratum corneum water content (actually measured value) in the skin test.

(Time comparison)
Comparison was made over time with the values before ingestion of the test food using actual measurements. As a result, compared with 49.70 ± 10.63 before intake in the test diet group, a statistically significant increase was observed at 58.97 ± 11.80 after 4 weeks and 59.47 ± 10.06 after 8 weeks (both p < 0.01 ).

Table 2 shows the results of the stratum corneum water content (variation) in the skin test.

(Comparison between groups)
A comparison between groups was performed using the amount of change from before ingestion. As a result, after 4 weeks of intake, a statistically significant difference was observed in the test diet group (9.26±5.55) compared with the placebo group (3.09±10.04) (p<0.05). Moreover, after 8 weeks of ingestion, a statistically significant difference was observed in the test diet group (9.77±6.30) compared with the placebo group (4.21±6.95) (p<0.05).

Table 3 shows the results of wrinkles and color unevenness (measured values) in skin measurement (VISIA-number).

(Time comparison)
As a result of successive comparison with before intake using the measured values, significant differences were observed in the following items.
Color unevenness: A statistically significant decrease was observed at 267±123 after 8 weeks of ingestion compared to 352±180 before ingestion in the test diet group (p<0.05).

Table 4 shows the results of wrinkles and color unevenness (variation) in skin measurement (VISIA-number).

(Comparison between groups)
As a result of inter-group comparison using the amount of change from before ingestion, significant differences were observed in the following items.
Wrinkles: After 8 weeks of ingestion, a significant difference was observed in the test diet group -2.40±3.91 compared to the placebo group 0.07±2.02 (p<0.05).
Color unevenness: After 8 weeks of ingestion, a significant difference was observed in the test diet group -85.3.±98.0 compared to the placebo group -7.00±55.9 (p<0.05).

This study confirmed that continuous ingestion of the yeast extract containing nucleotides and polyamines for 8 weeks increased the moisture content of the skin and reduced wrinkles and color unevenness.

Claims (2)

A skin yeast extract for oral use, containing 1 to 15% by weight of at least one nucleotide selected from nucleotides consisting of cytidylic acid, uridylic acid, guanylic acid, or adenylic acid, and a polyamine. Yeast extract for moisturizing or reducing wrinkles and uneven color. 2. The yeast extract for moisturizing skin or reducing wrinkles and uneven color according to claim 1, wherein the yeast is Candida utilis or Saccharomyces cerevisiae.