JP7253283B2 - Cosmetics and Food and Beverage Compositions - Google Patents

Description

TECHNICAL FIELD The present invention relates to novel compositions obtained by fermentation, anti-inflammatory agents, anti-aging agents, whitening agents, hair growth agents, cosmetics, and food and beverage compositions using the same.

Causes and development of inflammatory diseases such as contact dermatitis (rash), psoriasis, pemphigus vulgaris, atopic dermatitis, various skin inflammatory diseases accompanied by rough skin, rheumatoid arthritis, osteoarthritis, asthma, etc. Mechanisms vary widely. It is known that the cause is mainly due to enhancement of hyaluronidase activity, tumor necrosis factor (TNF-α) produced from macrophages, and the like.

Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. Hyaluronate, which maintains affinity for body tissue, is decomposed by ultraviolet light, oxygen, etc. in a water-containing system, and its water-retaining effect decreases as its molecular weight decreases. In addition, hyaluronic acid exists as intercellular tissue in vivo and is also involved in vascular permeability. Furthermore, hyaluronidase, which is present in mast cells, is released by degranulation caused by its activation and acts as an inflammatory chemical mediator. Therefore, inhibition of hyaluronidase activity is expected to enhance moisturization and prevent/reduce inflammation. As substances having hyaluronidase activity inhibitory action, for example, extracts from plants of the genus Osbechia (see Patent Document 1) are known.

TNF-α was found as a factor causing tumor necrosis, but recently it is considered to be a cytokine that plays a mediator role in regulating not only tumor but also normal cell functions. TNF-α plays an important role in the process from the onset to the end of inflammation, but its persistent and excessive production causes damage to tissues including the skin, and systemically causes fever and chalkia. resulting in exacerbation of inflammation. Therefore, suppression of excessive production of TNF-α is important in pathological inflammation. For example, extracts from mother earthen shells (see Patent Document 2) are known to have a TNF-α production inhibitory effect.

The epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as collagen, elastin, and hyaluronic acid that are outside these cells and support the skin structure. In young skin, the proliferation of fibroblasts is active, and the interaction of skin tissue such as fibroblasts and extracellular matrix components maintains homeostasis, ensuring moisture retention, flexibility, elasticity, etc. The appearance is maintained in a fresh state with tension and luster.

However, under the influence of certain external factors, such as exposure to ultraviolet rays, extreme dryness of the air, and excessive skin washing, or with advanced aging, collagen, elastin, and hyaluron, which are the main constituents of the extracellular matrix, As the amount of acid produced decreases, it causes decomposition and alteration. As a result, the moisturizing function and elasticity of the skin are reduced, and abnormal exfoliation of keratin occurs, so that the skin loses its firmness and luster, and exhibits aging symptoms such as rough skin and wrinkles. Thus, changes associated with aging of the skin, ie, wrinkles, dullness, changes in texture, reduction in elasticity, etc., are associated with reduction or denaturation of matrix components such as collagen, elastin, and hyaluronic acid. Therefore, promoting the production of collagen, elastin or hyaluronic acid is important in preventing, treating or improving skin aging.

Here, among these extracellular matrix components, elastin is a fiber that gives elasticity to skin tissue. If the production of elastin can be promoted, wrinkles and sagging are less likely to occur, and it is thought possible to prevent, treat, or ameliorate skin aging symptoms such as loss of tension and loss of elasticity.

In addition to skin tissue, elastin is widely expressed in tissues that require elasticity in the body, such as lungs and blood vessels. It is known that the decrease in normal elastin from these tissues with aging reduces the elasticity of the lungs and blood vessels, leading to pulmonary diseases such as emphysema, hypertension, and vascular diseases such as aneurysms. ing. Therefore, if the production of elastin can be promoted, the elasticity of the lungs and blood vessels is less likely to decrease, and pulmonary diseases such as emphysema, hypertension, and vascular diseases such as aneurysms can be prevented and treated. . For example, extracts from plants belonging to the genus Hippophae of the family Gummiaceae are known to have elastin production-promoting activity (see Patent Document 3).

On the other hand, among the extracellular matrix components described above, hyaluronic acid is a type of mucopolysaccharide, and has the function of retaining cells by being filled in the intercellular spaces, and further retaining water in the intercellular spaces. It has many functions such as lubricity and flexibility to tissues, and resistance to external forces such as mechanical damage. If the production of hyaluronic acid can be promoted, skin aging symptoms such as rough skin, wrinkles, dullness, changes in texture, loss of elasticity and loss of moisturizing function can be prevented, treated or improved. In addition, by promoting the expression of hyaluronic acid synthase 3 (HAS3) involved in promoting the synthesis of epidermal hyaluronic acid, it is believed that skin aging can be prevented, treated or improved.

In addition to skin tissue, hyaluronic acid is present in cartilage, synovial fluid, umbilical cord, vitreous body of the eye, and other connective tissues. Among them, hyaluronic acid contained in synovial fluid covers the surface of articular cartilage and contributes to the smooth operation of joints due to its lubricating function, covering and protecting cartilage, and the like. On the other hand, in arthritis such as rheumatoid arthritis, it is known that the concentration of hyaluronic acid in synovial fluid is decreased. Therefore, by promoting the production of hyaluronic acid, arthritis such as rheumatoid arthritis, osteoarthritis, septic arthritis, gouty arthritis, traumatic arthritis, or osteoarthritis can be prevented or treated. Furthermore, it is known that granulation (tissue) is formed during the healing process of wounds or burns, and hyaluronic acid is significantly increased in the granulation. Therefore, it is believed that promoting the production of hyaluronic acid can promote healing of wounds or burns. As a substance having a hyaluronic acid production-promoting effect, an extract from Camphorella camphorata (see Patent Document 4) and the like are known. In addition, licorice leaf extract (see Patent Document 5) and the like are known to have a hyaluronic acid synthase 3 (HAS3) mRNA expression promoting effect.

On the other hand, among the aforementioned extracellular matrix components, collagen is a fibrous protein that contributes to maintaining the structure and mechanical strength of skin tissue. If the production of collagen can be promoted, it is possible to prevent, treat, or improve aging symptoms such as wrinkles and sagging, rough texture, loss of tension, and decreased elasticity. Conceivable.

Also, collagen is abundantly present in bones, tendons, ligaments, corneas, blood vessels, etc., and it is known that a decrease in collagen production due to aging causes osteoporosis and the like. Furthermore, it is known that during the wound healing process, the amount of collagen produced is increased, and it serves as a scaffold for fibroblasts and the like, thereby promoting wound healing. Therefore, promotion of collagen production is also important from the viewpoint of prevention or treatment of osteoporosis and the like and promotion of wound healing. For example, an extract from Camphorella camphorata (Patent Document 4 mentioned above) is known as a substance having a collagen production-promoting action.

The epidermis has the function of relieving external stimuli and controlling the loss of body components such as water. It has a four-layer structure starting from the basal layer, which is the lowest layer, followed by the stratum spinosum, the stratum granulosum, and the stratum corneum. consists of Most of the cells present in each layer are keratinocytes differentiated from the basal layer. The keratinocytes, which divide and proliferate in the basal layer, differentiate while passing through the stratum spinosum and the stratum granulosum to become keratinocytes. Eventually, it falls off from the stratum corneum as dirt.

The stratum corneum is the outermost layer of the skin and serves as a physical barrier against external stimuli. In order for the skin to have this barrier function, the cycle (keratinization) from the production of keratinocytes in the basal layer to the exfoliation of the skin as dirt (keratinization) is repeated in a cycle of 4 weeks, and the metabolism of the epidermis is carried out. . However, this stratum corneum also loses its metabolic function with aging, and causes skin troubles such as wrinkles, dullness, pigmentation, and rough skin. Therefore, it is thought that skin aging such as wrinkles, dullness and pigmentation can be improved by promoting the proliferation of keratinocytes and restoring the metabolic function of the skin. Conventionally, an extract of mother earthen shell (see Patent Literature 2 mentioned above) and the like have been known as substances having an effect of promoting proliferation of epidermal keratinocytes.

Among the stratum basale, stratum spinosum, stratum granulosum, and stratum corneum, which constitute the epidermis, the cell membrane of the stratum granulosum in particular thickens to form a thickened cell membrane. Intermolecular glutamyl-lysine cross-links form tough keratin protein fibers. Furthermore, ceramide or the like is covalently bonded to a part of it to form a hydrophobic structure, which provides a base for the lamellar structure of intercellular lipids and forms the basis for the stratum corneum barrier function.

However, when the amount of transglutaminase-1 produced in the epidermis decreases with aging, the keratin barrier function and the moisturizing function of the skin decrease, resulting in skin aging symptoms such as rough skin and dry skin, and dry skin diseases ( For example, atopic dermatitis, psoriasis, ichthyosis, etc.) will develop. Therefore, it is thought that aging symptoms of the skin, dry skin diseases, etc. can be prevented, treated or improved by promoting the production of transglutaminase-1 in the epidermis. An extract from Hunan sweet tea (see Patent Document 6) and the like are known to have a transglutaminase-1 production-promoting effect.

Ceramide is produced from serine and palmitoyl-CoA in the process of keratinization of epidermal cells through the actions of enzymes including serine palmitoyltransferase (SPT) known as the rate-limiting enzyme for ceramide synthesis. Ceramide exists specifically as a major component of intercorneocyte lipids that cover the outermost layer of the skin, and plays an important role in maintaining the skin's original function as a barrier film between the living body and the outside world.

The structure of the stratum corneum is likened to a brick and a mortar, and forms a strong barrier film in the form of intercellular lipids connecting corneocytes, which are piled up in about 15 layers. Corneocytes retain moisture by containing natural moisturizing factors whose main components are amino acids, while intercorneocyte lipids are mainly composed of about 50% ceramide, and contain cholesterol, fatty acids, and other parent substances. It is composed of lipophilic lipids and is characterized by a so-called lamellar structure in which hydrophobic and hydrophilic portions are alternately repeated.

Deterioration of the skin barrier function due to various internal and external factors increases transepidermal water loss, causing dry skin, desquamation, itching, and the like, resulting in so-called dry skin. In addition, the deterioration of the skin barrier function leads to a vicious cycle in which skin inflammation increases and the defense function against various external stimuli decreases. In recent studies, it has been reported that keratin ceramide components (so-called intercellular lipids) decrease or change in composition due to aging or in patients with atopic dermatitis known as barrier disorder (see Non-Patent Document 1). It has become widely known that ceramide is important for maintaining and improving the barrier function of the skin. Known methods for improving the barrier function of the skin include a method of supplementing ceramide from the outside (see Non-Patent Document 2) and a method of increasing ceramide-producing ability inside the skin (see Non-Patent Document 3).

It is known that in skin cells, aquaporins, known as water channels, are expressed on the cell membrane and play a role in the uptake of low-molecular-weight substances, including intercellular water, into the cells. In humans, 13 kinds of aquaporins (AQP0 to AQP12) are known to exist. Epidermal cells mainly contain AQP3, which is thought to play a role in taking up not only water but also low-molecular-weight compounds such as glycerol and urea that are involved in water retention.

However, AQP3 decreases with age, and it has been suggested that this is one of the reasons for the decline in water retention function. can be controlled (see Non-Patent Document 4). For example, an extract from star fruit leaves (see Patent Document 7) is known to have an AQP3 expression promoting effect.

Glutathione is a tripeptide consisting of three amino acids, glutamic acid, cysteine and glycine, and is the compound with the major cysteine residue in cells. Glutathione in cells functions as a radical scavenger, regulates cell functions by oxidation-reduction, metabolizes foreign substances, and acts as an SH donor for various enzymes, and is also known as an antioxidant component against active oxygen and the like. Its action is believed to be derived from cysteine residues. However, it has been reported that the amount of intracellular glutathione is depleted or decreased due to excessive oxidative stress, addition of foreign substances, aging, etc. This reduces the defense ability of cells against oxidative stress, and increases the DNA content of cells. and is considered to be one of the causes of damage to constituents such as proteins.

Diseases in which a decrease or deficiency in the amount of intracellular glutathione is known to be associated with pathological conditions include pigmentation such as skin spots, aging of the skin, and oxidative stress. In addition to the group of diseases listed above, liver disorders (caused by excessive alcohol consumption or ingestion of foreign substances such as heavy metals and chemical substances) are known. That is, promoting the production of glutathione is thought to increase the ability of cells to defend against oxidative stress, and prevent and treat the above-mentioned groups of diseases caused by a decrease or deficiency in the amount of intracellular glutathione. . Amber hot water extract (see Patent Document 8) and the like are known to have a glutathione production promoting effect.

Hair repeats growth and shedding according to a periodic hair cycle (hair cycle) consisting of a growth phase, a regression phase and a telogen phase. Within this hair cycle, the stage from the resting phase to the anagen phase, in which new hair follicles are formed, is considered to be the most important for hair growth. Dermal papilla cells are thought to play an important role. Dermal papilla cells are cells located inside hair follicle epithelial cells consisting of outer root sheath cells and matrix cells in the vicinity of the hair root, and located at the root portion of the hair root surrounded by the basement membrane. It plays an important role in proliferation/differentiation of hair follicle epithelial cells and hair formation, such as acting on epithelial cells to promote their proliferation (see Non-Patent Document 5).

Thus, dermal papilla cells play an important role in the proliferation and differentiation of hair follicle epithelial cells and hair formation, and promoting the proliferation of dermal papilla cells can prevent and improve alopecia. It is possible. So far, for example, wild thyme extract (see Patent Document 9) and the like are known to have an effect of promoting the proliferation of dermal papilla cells.

Problems related to hair include those related to the state of hair roots and hair follicles such as hair loss and thinning hair, as well as those related to hair quality such as hard, soft, thin, lack of elasticity, split ends, and frizzy hair. . Furthermore, problems related to the quality of hair are caused by hair damage caused by daily hair care, hair makeup, exposure to ultraviolet rays, etc., and by problems in hair shaft formation. ing.

Hair is composed of a cuticle covering its surface, a cortex inside it, and a medulla occupying the center of the hair. Among these, it is known that the cuticle that covers the surface of the hair is most susceptible to damage to the hair, and that the cuticle plays an important role in the hardness, stiffness, stiffness, etc. of the hair (for example, , Non-Patent Document 6). Therefore, if the cuticle can be effectively regenerated, it is considered possible to improve the hair quality such as hardness, elasticity, and stiffness of the hair.

Here, recently, among keratin-associated proteins (KAP), it has been clarified that there is a correlation between the mRNA expression level of the KAP5 family gene and the strength of hair elasticity and stiffness (for example, , see Patent Document 10). It has also been reported that KAP5.1, one of the KAP5 families, is localized in the growing cuticle (see, for example, Non-Patent Document 7). Therefore, if the expression of the KAP5 family, especially KAP5.1 mRNA can be promoted, it is expected to lead to regeneration of the cuticle and, in turn, improvement of hair quality such as hardness, elasticity, and stiffness of the hair.

Under such circumstances, a licorice leaf extract or the like has been reported as a substance that promotes the expression of KAP5.1 mRNA (see Patent Document 11).

JP 2003-055242 A JP 2006-056854 A JP-A-2005-022993 JP-A-2003-146837 JP 2010-090035 A JP 2007-099698 A JP 2009-191039 A JP 2010-235551 A JP 2006-219407 A JP 2006-014721 A JP 2013-193959 A

J. Dermatol., 1993, Vol.20, No.1, p.1-6 "Fragrance Journal", 2004, Vol.32, No.11, p.23-32 Br. J. Dermatol., 2000, Vol.143, Issue 3, p.524-531 "Fragrance Journal", 2006, Vol.34, No.10, p.19-23 Trends Genet., 1992, Vol.8, Issue 2, p.55-61 Atsushi Sogabe et al., Journal of the Society of Cosmetic Engineers of Japan, 2002, Vol.36, No.3, p.207-216 Jones LN. et al., Int J Trichology, 2010, Vol.2, No.2, p.89-95

An object of the present invention is to provide a novel composition derived from a natural product and having excellent anti-inflammatory, anti-aging, whitening or hair-restoring effects. In addition, the present invention provides an anti-inflammatory agent, an anti-aging agent, a whitening agent, and a hair restorer containing the novel composition as an active ingredient, as well as cosmetic and food and beverage compositions containing the novel composition. is a further object.

In order to solve the above problems, the present invention provides a fermented extract of Tremella fungus as a natural product-derived component. Further, the anti-inflammatory agent, anti-aging agent, whitening agent and hair restorer of the present invention are characterized by containing a fermented extract of Tremella fungus as an active ingredient. Furthermore, the cosmetic and food/drink compositions of the present invention are characterized by blending a fermented extract of Tremella fungus.

INDUSTRIAL APPLICABILITY According to the present invention, it is possible to provide a fermented Tremella fungus extract as a natural product-derived component excellent in anti-inflammatory action, anti-aging action, whitening action or hair growth action. Moreover, by using the fermented extract of Tremella as an active ingredient, it is possible to provide an anti-inflammatory agent, an anti-aging agent, a whitening agent and a hair restorer with excellent effects. Furthermore, by blending the fermented extract of Tremella fungus, it is possible to provide cosmetics and food and beverage compositions that are excellent in the above effects.

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described below.
[Fermented tremella extract]
The fermented extract of Tremella Tremella according to the present embodiment is a fermented product of an extract from Tremella tremella by microorganisms.

Here, unless otherwise specified, the term “tremella fungus extract fermented product” in the present specification includes a fermentation broth obtained by using a tremella fungus extract as a fermentation raw material, a diluted or concentrated solution of the fermentation broth, and the fermentation broth. Dried products obtained by drying, or crudely purified products or purified products thereof are included. In addition, the "white fungus extract" in this specification includes an extract obtained from white fungus as an extraction raw material, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, or a crude Both purified and purified products are included.

The raw material used in this embodiment is white fungus (scientific name: Tremella fuciformis).

Tremella fuciformis (scientific name: Tremella fuciformis) is a basidiomycete (mushroom) belonging to the genus Tremella of the family Tremella, is edible in Japan and China, and can be easily obtained from these regions. The part used as a raw material is not particularly limited and can be appropriately selected according to the purpose, but the fruiting body is preferred.

The extract from the white fungus can be obtained by drying the raw material for extraction, either as it is or pulverizing it using a coarse crusher, and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. In addition, it may be used as an extraction raw material after being subjected to pretreatment such as degreasing with a non-polar solvent such as hexane. By performing pretreatment such as degreasing, the extraction treatment with a polar solvent can be efficiently performed.

As the extraction solvent, it is preferable to use a polar solvent, for example, water, hydrophilic organic solvents, etc., which are used alone or in combination of two or more at room temperature or at a temperature below the boiling point of the solvent. is preferred.

Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Treatments applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmotic adjustment, buffering, and the like. Therefore, water that can be used as an extraction solvent in the present embodiment includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered physiological saline, and the like.

Hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

When using a mixture of two or more polar solvents as the extraction solvent, the mixture ratio can be adjusted as appropriate. For example, when a mixture of water and a lower aliphatic alcohol is used as the extraction solvent, the mixing ratio of water and the lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), More preferably, it is 7:3 to 2:8 (volume ratio). When using a mixture of water and lower aliphatic ketone, the mixing ratio of water and lower aliphatic ketone is preferably 9:1 to 2:8 (volume ratio). When using a mixed solution with a hydric alcohol, the mixing ratio of water and polyhydric alcohol is preferably 7:3 to 1:9 (volume ratio).

The extraction treatment is not particularly limited as long as the soluble components contained in the raw material for extraction can be eluted into the extraction solvent, and can be carried out according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent of 5 to 15 times the amount (mass ratio) of the extraction raw material, and the soluble components are extracted at room temperature, under reflux heating or under pressure, and then filtered to remove the extraction residue. Thus, an extract can be obtained. A paste-like concentrate is obtained by distilling off the solvent from the obtained extract, and a dried product is obtained by further drying the concentrate.

The white fungus extract obtained as described above is subjected to fermentation by microorganisms. Fermentation treatment can be carried out, for example, by dissolving a concentrated and dried white fungus extract in water, or by using the white fungus extract as it is without concentrating and drying, and inoculating the fermenting microorganism.

Microorganisms that perform fermentation are not particularly limited, and examples thereof include lactic acid bacteria, yeast, and koji mold. Specifically, the lactic acid bacteria include Lactobacillus plantarum, Lactobacillus casei and other genus Lactobacillus; Examples include lactic acid bacteria belonging to the genus Bifidobacterium such as Bifidobacterium breve. Examples of yeast include Saccharomyces genus such as Saccharomyces cerevisiae (baker's yeast) and Saccharomyces veronae; and Schizosaccharomyces genus such as Schizosaccharomyces pombe Kluyveromyces genus such as Kluyveromyces thermotolerans; Zygosaccharomyces genus such as Zygosaccharomyces thermotolerans; Lacanthea such as Lachancea thermotolerans and the like are preferably exemplified. Examples of koji mold include Aspergillus oryzae, Aspergillus sojae and other Aspergillus species; Monascus purpureus and other Monascus species, and the like. In this embodiment, these microorganisms can be used individually by 1 type or in combination of 2 or more types. When two or more kinds of microorganisms are used in combination, fermentation may be carried out simultaneously with two or more kinds of microorganisms, or primary fermentation with koji mold is first carried out, and then secondary fermentation with lactic acid bacteria and/or yeast is carried out. good too.

Among these, the microorganism used in the present embodiment is one of Lactobacillus plantarum and Saccharomyces veronae from the viewpoint of safety and the viewpoint of the action exhibited by the fermented product extracted from white fungus described later. Seeds or more are preferred.

Here, Lactobacillus plantarum is a kind of lactic acid bacteria, but it is mainly isolated from fermented vegetable foods such as vegetable pickles, kimchi, and miso, so it is included in vegetable lactic acid bacteria, and fermented milk such as yogurt and lactic acid bacteria. It is different from animal lactic acid bacteria used in the production of beverages. Saccharomyces veronae (also known as Lachancea thermotolerans) differs from other yeasts such as Saccharomyces cerevisiae in that it does not substantially undergo alcoholic fermentation.

Furthermore, when using Lactobacillus plantarum, Lactobacillus plantarum 22A-1 (FERM P-21409), Lactobacillus plantarum 22A-3 (FERM P-21411), and Lactobacillus plantarum 22B-2 (FERM P- 21410), and particularly preferably Lactobacillus plantarum 22A-3 (FERM P-21411). Here, the three strains of Lactobacillus plantarum 22A-1 (FERM P-21409), Lactobacillus plantarum 22A-3 (FERM P-21411), and Lactobacillus plantarum 22B-2 (FERM P-21410) are , is a strain isolated and identified from pickles by the applicant of the present application, and has been deposited at the Independent Administrative Institution National Institute of Advanced Industrial Science and Technology Patent Organism Depositary.

Fermentation treatment using the above-mentioned microorganisms is carried out, for example, by placing an aqueous solution of Tremella fungus extract and the above-mentioned microorganisms in a fermenter at a temperature range of 25 to 35 ° C., preferably 28 to 32 ° C., for 12 to 72 hours, preferably 18 to 18. It can be done by treating for 48 hours. Further, when the pH of the reaction solution is adjusted to 5.0 to 7.0, preferably 5.5 to 6.5 at the start of the fermentation process, the fermentation process proceeds favorably. Such fermentation treatment is completed by subjecting the fermented liquid to desired means such as cooling, heat sterilization, and filtration to inactivate the fermenting bacteria.

The fermented liquid thus obtained may be used as it is as a fermented product, or may be diluted or concentrated as appropriate and used as a fermented product. Furthermore, the concentrate may be further dried, and crude purification and the like may be performed.

In addition, before the extraction process and fermentation process described above, the raw material for extraction or the extract (raw material for fermentation) may be subjected to a process for increasing the efficiency of extraction or fermentation. Preferred examples of such treatment include enzymatic treatment using glucanase, cellulase, and the like. For example, it is particularly preferable to subject the extract obtained by the extraction treatment to an enzyme treatment, since the efficiency of the subsequent fermentation treatment is increased.

The fermented extract of Tremella fungus obtained in this manner exhibits excellent anti-inflammatory, anti-aging, whitening and hair growth effects, and therefore the active ingredient of the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer described below. as a component of cosmetics or food and drink compositions.

[Anti-inflammatory agent, anti-aging agent, whitening agent, hair restorer]
The anti-inflammatory agent, anti-aging agent, whitening agent and hair restorer according to the present embodiment contain the fermented extract of Tremella fungus obtained as described above as an active ingredient. The anti-inflammatory agent, anti-aging agent, whitening agent and hair restorer of this embodiment can be used in a wide range of applications such as pharmaceuticals, quasi-drugs and cosmetics.

Here, the anti-inflammatory effect of the fermented extract of white fungus is exerted based on, for example, hyaluronidase activity inhibitory effect and/or tumor necrosis factor (TNF-α) production inhibitory effect. However, the anti-inflammatory action of the fermented product of Tremella fungus extract is not limited to the anti-inflammatory action exhibited based on the above action. In addition, the fermented extract of white fungus may be used as an active ingredient of a hyaluronidase activity inhibitor or a TNF-α production inhibitor, respectively, by utilizing its hyaluronidase activity inhibitory action or TNF-α production inhibitory action.

The anti-aging effect of the fermented product of Tremella fungus extract includes, for example, elastin production promoting action, hyaluronic acid production promoting action, type IV collagen production promoting action, epidermal keratinocyte proliferation action, and transglutaminase-1 (TG-1) production promoting action. , profilaggrin mRNA expression promoting action, serine palmitoyltransferase (SPT) mRNA expression promoting action, aquaporin 3 (AQP3) mRNA expression promoting action and hyaluronic acid synthase 3 (HAS3) mRNA expression promoting action. Alternatively, it is exerted based on two or more types of action. However, the anti-aging action of the fermented product of the fungus extract is not limited to the anti-aging action exhibited based on the above action.

In addition, the fermented tremella fungus extract has elastin production-promoting action, hyaluronic acid production-promoting action, type IV collagen production-promoting action, epidermal keratinocyte proliferation action, TG-1 production-promoting action, profilaggrin mRNA expression-promoting action, and SPT mRNA expression. Elastin production promoter, hyaluronic acid production promoter, type IV collagen production promoter, epidermal keratinocyte proliferator, TG-1 production promoter, and It may be used as an active ingredient of profilaggrin mRNA expression promoter, SPT mRNA expression promoter, AQP3 mRNA expression promoter or HAS3 mRNA expression promoter.

The whitening effect of the fermented product of the fungus extract is exhibited based on, for example, the effect of promoting glutathione production. However, the whitening action of the fermented product of the fungus extract is not limited to the whitening action exhibited based on the above action. Moreover, the fermented extract of Tremella fungus may be used as an active ingredient of a glutathione production promoter by utilizing its glutathione production promoting action.

The hair-restoring action of the fermented extract of Tremella fungus is exerted based on, for example, dermal papilla cell proliferation-promoting action and/or keratin-associated protein 5.1 (KAP5.1) mRNA expression-promoting action. However, the hair-restoring action of the fermented extract of white fungus is not limited to the hair-restoring action exhibited based on the above action. In addition, the fermented extract of Tremella fungus may be used as an active ingredient of a dermal papilla cell proliferation-promoting agent or a KAP5.1 mRNA expression-promoting agent, respectively, by utilizing its dermal papilla cell proliferation-promoting action or KAP5.1 mRNA expression-promoting action. .

The anti-inflammatory agent, anti-aging agent, whitening agent, or hair restorer of the present embodiment may consist of only the fermented extract of Tremella fungus, or may be a formulation of the fermented extract of Tremella fungus.

The anti-inflammatory agent, anti-aging agent, whitening agent and hair restorer of the present embodiment can be prepared in the form of powder or granules using a pharmaceutically acceptable carrier such as dextrin, cyclodextrin or any other adjuvant according to a conventional method. , tablets, and liquids. At this time, as auxiliary agents, for example, excipients, binders, disintegrants, lubricants, stabilizers, flavoring agents and the like can be used. Anti-inflammatory agents, anti-aging agents, whitening agents and hair restorers can be used by blending with other compositions (for example, cosmetics, food and drink compositions described later), ointments, external liquids, It can be used as a patch or the like.

When the anti-inflammatory agent, anti-aging agent, whitening agent, or hair restorer of the present embodiment is formulated, the content of the fermented extract of Tremella fungus is not particularly limited, and can be appropriately set according to the purpose. .

It should be noted that the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer of the present embodiment may, if necessary, contain other natural extracts having anti-inflammatory, anti-aging, whitening or hair-restoring effects. It can be blended with the fermented extract and used as an active ingredient.

Methods of administering the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer of the present embodiment to patients include transdermal administration, oral administration and the like. A suitable method may be selected as appropriate. In addition, the dosage of the anti-inflammatory agent, anti-aging agent, whitening agent, or hair restorer of the present embodiment may also be appropriately increased or decreased depending on the type and severity of disease, individual patient differences, administration method, administration period, and the like.

The anti-inflammatory agent of the present embodiment has contact dermatitis (rash), psoriasis, pemphigus vulgaris, atopic dermatitis, psoriasis, pemphigus vulgaris, and atopic dermatitis through hyaluronidase activity inhibitory action and/or TNF-α production inhibitory action possessed by the fermented extract of white fungus, which is an active ingredient. It is possible to prevent, treat or ameliorate various skin inflammatory diseases associated with dermatitis and other rough skin. However, in addition to these uses, the anti-inflammatory agent of the present embodiment can be used for all uses that are significant in exhibiting hyaluronidase activity inhibitory action or TNF-α production inhibitory action.

For example, the anti-inflammatory agent of the present embodiment or the hyaluronidase activity inhibitor or TNF-α production inhibitor described above is effective for rheumatoid arthritis and osteoarthritis through the hyaluronidase activity inhibitory action or TNF-α production inhibitory action possessed by the fermented product of the white fungus extract. disease, asthma, etc., can be prevented, treated or ameliorated.

The anti-aging agent of the present embodiment has an elastin production-promoting action, a hyaluronic acid production-promoting action, a type IV collagen production-promoting action, an epidermal keratinocyte proliferation action, and a TG-1 production-promoting action possessed by the fermented extract of Tremella fungus, which is an active ingredient. , profilaggrin mRNA expression promoting action, SPT mRNA expression promoting action, AQP3 mRNA expression promoting action and HAS3 mRNA expression promoting action, through one or more actions selected from the group consisting of skin wrinkle formation, skin elasticity reduction, moisturizing function It can prevent, treat or improve skin aging symptoms such as a decrease in However, in addition to these uses, the anti-aging agent of the present embodiment has elastin production-promoting action, hyaluronic acid production-promoting action, type IV collagen production-promoting action, epidermal keratinocyte proliferation action, TG-1 production-promoting action, It can be used for all applications that are significant in exhibiting filaggrin mRNA expression-enhancing action, SPT mRNA expression-enhancing action, AQP3 mRNA expression-enhancing action, or HAS3 mRNA expression-enhancing action.

For example, the anti-aging agent of the present embodiment or the elastin production-enhancing agent described above is used for the prevention, treatment, or improvement of pulmonary diseases such as emphysema; vascular diseases such as hypertension and aneurysm; can be used for In addition, the anti-aging agent of the present embodiment or the hyaluronic acid production promoter described above is effective for rheumatoid arthritis, osteoarthritis, septic arthritis, gouty arthritis, traumatic arthritis, osteoarthritis, etc., due to its hyaluronic acid production promoting action. prevention, treatment or amelioration of arthritis; promotion of healing of wounds or burns; Furthermore, the anti-aging agent of the present embodiment or the collagen production promoter described above prevents, treats, or improves diseases caused by decreased collagen production such as osteoporosis through the type IV collagen production-promoting action of the fermented extract of white fungus; It can be used for applications such as promotion of regeneration of injured tendons and ligaments; promotion of healing of wounds or burns.

In addition to the above-mentioned uses, the anti-aging agent of the present embodiment or the above-mentioned epidermal keratinocyte proliferation-promoting agent restores skin metabolism through the epidermal keratinocyte proliferation-promoting action possessed by the fermented product of the Tremella fungus extract, It can be used for purposes such as prevention, treatment or improvement of dullness and pigmentation; regenerative medicine;

In addition to the above-mentioned uses, the anti-aging agent of the present embodiment or the above-mentioned TG-1 production promoter or SPTmRNA expression promoter has the effect of promoting TG-1 production or SPTmRNA expression, which is possessed by the fermented product of the white fungus extract. It can strengthen the barrier function and prevent, treat, or improve rough skin, dry skin, and dry skin diseases (eg, atopic dermatitis, psoriasis, ichthyosis, etc.). In addition, the anti-aging agent of the present embodiment or the AQP3 mRNA expression promoter described above can improve age-related water retention capacity, barrier function, etc. through the AQP3 mRNA expression promoting action of the fermented product of the Tremella fungus extract.

The whitening agent of the present embodiment can prevent and improve pigmentation such as darkening of the skin, dark spots, and freckles through the glutathione production-promoting action of the fermented extract of white fungus, which is the active ingredient. However, the whitening agent of the present embodiment can be used for all uses other than these uses that are significant in exhibiting the action of promoting glutathione production.

For example, the whitening agent of the present embodiment or the glutathione production enhancer described above can be used to promote glutathione production of the fermented extract of the white fungus extract, through liver damage (drinking alcohol excessively, or intake of foreign substances such as heavy metals and chemicals). It is possible to prevent, treat, or ameliorate diseases in which a decrease or deficiency of intracellular glutathione is known to be associated with pathology, such as

The hair restorer of the present embodiment prevents, treats, or improves alopecia such as androgenic alopecia, alopecia areata, and trichotillomania through the dermal papilla cell proliferation-promoting action of the fermented extract of Tremella fungus, which is an active ingredient. It is particularly suitable for preventing, treating or improving male pattern baldness. In addition, the hair restorer of the present embodiment can improve the quality of hair, particularly the elasticity and stiffness of hair, through the KAP5.1 mRNA expression-enhancing action of the fermented extract of white fungus, which is an active ingredient. However, the hair restorer of the present invention can be used for all uses other than these uses that are significant in exhibiting the dermal papilla cell proliferation-promoting action or the KAP5.1 mRNA expression-promoting action.

For example, the hair restorer of the present embodiment or the aforementioned dermal papilla cell proliferation-promoting agent can be applied to the field of regenerative medicine such as hair regeneration using dermal papilla cells through the dermal papilla cell proliferation-promoting action of the fermented extract of Tremella fungus. can also be used.

In addition, since the anti-inflammatory agent, anti-aging agent, whitening agent, or hair restorer of the present embodiment has excellent anti-inflammatory, anti-aging, whitening, or hair-growth effects, it can be blended, for example, in external preparations for skin. preferred. In this case, the fermented product of the fungus extract may be blended as it is, or an anti-inflammatory agent, an anti-aging agent, a whitening agent, or a hair restorer formulated from the fermented product of the fungus extract may be blended.

Here, the category of external preparations for skin is not limited, and includes a wide range of skin cosmetics, quasi-drugs used transdermally, pharmaceuticals, and the like, which will be described later.

In addition, since the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer of the present embodiment has excellent anti-inflammatory action, anti-aging action, whitening action or hair growth action, reagents for research on these mechanisms of action It can also be suitably used as

[Cosmetics]
The cosmetic of the present embodiment contains the above-described fermented extract of Tremella fungus. The cosmetic according to the present embodiment may contain the fermented extract of Tremella as it is, or may contain an anti-inflammatory agent, an anti-aging agent, a whitening agent, or a hair restorer formulated from the fermented extract of Tremella. .

The fermented product of Tremella fungus extract, or the type of cosmetics in which the above anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer can be blended is not particularly limited, but the action of the fermented product of Tremella fungus extract is exhibited more effectively. It is preferably a skin cosmetic or a hair cosmetic, from the viewpoint of making the skin smoother. The form of skin cosmetics or hair cosmetics is not particularly limited. include, for example, hair tonics, hair creams, hair liquids, shampoos, pomades, rinses and the like.

When the fermented tremella fungus extract, or the above anti-inflammatory agent, anti-aging agent, whitening agent, or hair restorer is blended into the cosmetic, the blending amount can be appropriately adjusted according to the type of the cosmetic, but is preferably The blending ratio is about 0.0001 to 10% by mass in terms of the solid content of the fermented extract of the fungus extract, and a particularly preferred blending ratio is about 0.001 to 1% by mass.

The cosmetic of this embodiment has an anti-inflammatory action, TNF-α production inhibitory action, hyaluronidase activity inhibitory action, anti-aging action, elastin production promotion action, hyaluronic acid production promotion action, and type IV collagen production promotion that the fermented product of Tremella fungus extract has. action, epidermal keratinocyte proliferation action, transglutaminase-1 production promotion action, profilaggrin mRNA expression promotion action, SPT mRNA expression promotion action, AQP3 mRNA expression promotion action, HAS3 mRNA expression promotion action, whitening action, glutathione production promotion action, hair growth action, Main ingredients, auxiliaries or other ingredients used in the production of ordinary cosmetics, such as astringents, bactericidal / antibacterial agents, whitening agents, as long as they do not interfere with the action of promoting dermal papilla cell proliferation or the action of promoting KAP5.1 mRNA expression. UV absorbers, moisturizers, cell activators, anti-inflammatory/anti-allergic agents, antioxidants/active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, etc. can be used together. Such combination may result in a more generalized product and may provide greater benefits than would normally be expected due to synergy between the other active ingredients used in combination.

When the fermented white fungus extract or the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer is incorporated in cosmetics, it exhibits anti-inflammatory action, TNF-α production inhibitory action, hyaluronidase activity inhibitory action, anti-aging action, promotion of elastin production, promotion of hyaluronic acid production, promotion of type IV collagen production, proliferation of epidermal keratinocytes, promotion of transglutaminase-1 production, promotion of profilaggrin mRNA expression, promotion of SPT mRNA expression, promotion of AQP3 mRNA expression, HAS3 mRNA expression promoting action, whitening action, glutathione production promoting action, hair growth action, dermal papilla cell proliferation promoting action, or KAP5.1 mRNA expression promoting action can be imparted to cosmetics.

All of these actions exhibit favorable actions when applied to cosmetics. Among them, when the cosmetic is a skin cosmetic, anti-inflammatory action, hyaluronidase activity inhibitory action, and TNF-α Production inhibitory action, anti-aging action, elastin production promotion action, hyaluronic acid production promotion action, type IV collagen production promotion action, epidermal keratinocyte proliferation action, transglutaminase-1 production promotion action, profilaggrin mRNA expression promotion action, SPT mRNA expression It is particularly preferable to impart a stimulating action, AQP3 mRNA expression promoting action, HAS3 mRNA expression promoting action, whitening action, or glutathione production promoting action to the skin cosmetic, since these actions are likely to be exhibited.

In addition, when the cosmetic is a hair cosmetic, hair growth action, dermal papilla cell proliferation promotion action, KAP5.1 mRNA expression promotion action, anti-inflammatory action, hyaluronidase activity inhibitory action, TNF-α production inhibitory action, and anti-aging action. , elastin production-promoting action, hyaluronic acid production-promoting action, type IV collagen production-promoting action, or HAS3 mRNA expression-promoting action is imparted to the hair cosmetic, since these actions are easily exhibited, and thus it is particularly preferable.

The cosmetic of this embodiment has an anti-inflammatory action, TNF-α production inhibitory action, hyaluronidase activity inhibitory action, anti-aging action, elastin production promotion action, hyaluronic acid production promotion action, and type IV collagen production promotion that the fermented product of Tremella fungus extract has. action, epidermal keratinocyte proliferation action, transglutaminase-1 production promotion action, profilaggrin mRNA expression promotion action, SPT mRNA expression promotion action, AQP3 mRNA expression promotion action, HAS3 mRNA expression promotion action, whitening action, glutathione production promotion action, hair growth action, Contact dermatitis (rash), psoriasis, pemphigus vulgaris, various types of skin associated with rough skin through one or more actions selected from the group consisting of dermal papilla cell proliferation promoting action and KAP5.1 mRNA expression promoting action Prevention, treatment, or improvement of inflammatory diseases; prevention, treatment, or improvement of skin aging symptoms such as wrinkle formation, decreased elasticity, and decreased moisturizing function; rough skin, dry skin, etc., and dry skin diseases Prevention, treatment or improvement of (e.g., atopic dermatitis, psoriasis, ichthyosis, etc.); Prevention, treatment or improvement of pigmentation such as skin darkening, age spots, freckles; Androgenetic alopecia, alopecia areata, trichoderma Prevention, treatment or improvement of alopecia such as tiromania; improvement of hair elasticity and elasticity; and the like.

[Food and drink composition]
The food and drink composition of the present embodiment contains the aforementioned fermented extract of Tremella fungus. The food and drink composition according to the present embodiment may contain the fermented extract of Tremella as it is, or may contain an anti-inflammatory agent, an anti-aging agent, a whitening agent, or a hair restorer formulated from the fermented extract of Tremella fungus. good too.

Here, “food and drink” refers to those that are less likely to harm human health and are taken orally or through the gastrointestinal tract in normal social life. It is not limited to categories such as Therefore, the "food and beverage composition" in the present embodiment includes orally ingested general foods, health foods (functional foods and drinks), and foods with health claims (foods for specified health uses, foods with nutrient claims, foods with functional claims). , quasi-drugs, pharmaceuticals, etc. The food and drink composition according to the present embodiment is preferably a food and drink made of the composition or a composition that constitutes a food and drink in which the preferred effects of the fermented product of the fungus extract are indicated on the package. It is particularly preferable that the product is a food with health claims (food for specified health use, food with function claims, food with nutrient function claims), quasi-drugs, and pharmaceuticals.

When the fermented product of Tremella fungus extract, or the anti-inflammatory agent, anti-aging agent, whitening agent or hair restorer formulated from the fermented product of Tremella fungus extract is blended into food and drink compositions, the amount of the active ingredient in them depends on the purpose of use, symptoms , Although it can be changed as appropriate in consideration of gender, etc., considering the general intake of the food and drink composition to be added, the extract intake per day for adults will be about 1 to 1000 mg. It is preferable to In addition, when the food and drink composition to be added is a granular, tablet or capsule-shaped food and drink composition, an anti-inflammatory agent, anti-aging agent, whitening agent or The amount of the hair restorer to be added is usually 0.1 to 100% by mass, preferably 5 to 100% by mass, relative to the food or drink composition to which it is added.

The food and drink composition of the present embodiment may be any food or drink composition that does not interfere with the activity of the fermented Tremella fungus extract, or may be a nutritional supplement containing the fermented Tremella fungus extract as a main ingredient. Food may be used.

When producing the food and drink composition of the present embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; polysaccharides of; soybean oil, oils and fats such as medium-chain fatty acid triglycerides can be added to make food and drink compositions of any shape.

The food and drink composition in which the fermented product of the fungus extract can be blended is not particularly limited, but specific examples include beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (concentrated undiluted solutions and preparations of these drinks). frozen desserts such as ice cream, ice sherbet, and shaved ice; noodles such as buckwheat, udon, vermicelli, gyoza skin, shumai skin, Chinese noodles, instant noodles; candy, chewing gum, candy, gum, chocolate, Confectionery such as tablets, snacks, biscuits, jellies, jams, creams, and baked goods; processed marine and livestock foods such as fish cakes, hams, and sausages; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, Oils and fats such as mayonnaise, shortening, whipped cream, and dressings; seasonings such as sauces and sauces; soups, stews, salads, side dishes, and pickles; health and nutritional supplements in various forms; tablets, capsules, drinks and the like. When the fermented extract of Tremella fungus is incorporated into these food and drink compositions, commonly used supplementary raw materials and additives can be used together.

The anti-inflammatory agent, anti-aging agent, whitening agent, hair restorer, cosmetic, and food and drink composition of the present embodiment are preferably applied to humans, but their respective effects are achieved. It can also be applied to non-human animals (eg, mice, rats, hamsters, dogs, cats, cows, pigs, monkeys, etc.) as long as they are available.

The present invention will be specifically described below with reference to Production Examples and Test Examples, but the present invention is not limited to the following examples.

[Production Example 1] Production of fermented product of lactic acid bacteria extracted from Tremella fungus Water (300 mL) is added to dried Tremella fungus (9 g), extraction is performed at 80 to 90 ° C. for 2 hours using a reflux condenser, and then sterilization is performed. to obtain a white fungus extract (300 g).

Lactobacillus plantarum 22A-3 strain (accession number: FERM P-21411) was inoculated into the resulting white fungus extract (100 g) and fermented at 30° C. for 24 hours. The resulting fermented liquid was filtered, and the filtrate was concentrated to dryness to obtain a fermented product of lactic acid bacteria extracted from white fungus (1.6 g, sample 1).

[Production Example 2] Production of fermented product with yeast extract from Tremella fungus Using the extract obtained in the process of Production Example 1, yeast for fermentation (Saccharomyces verona, manufactured by Akita Konno Shoten Co., Ltd.) was added to the extract (100 g) of Tremella japonicum. Inoculated and fermented at 30° C. for 24 hours. The resulting fermented liquid was filtered, and the filtrate was concentrated to dryness to obtain a fermented product of white fungus extract yeast (1.5 g, sample 2).

[Test Example 1] Hyaluronidase activity inhibitory action test The lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of yeast extract of Tremella tremosa obtained in Production Example 2 (Sample 2) were treated as follows. Hyaluronidase activity inhibitory action was tested.

Hyaluronidase solution (manufactured by SIGMA, Type IV-S, from bovine tests, 400 NF units/mL) was added and allowed to stand at 37°C for 20 minutes. Furthermore, 0.2 mL of 2.5 mmol/L calcium chloride was added as an activator, and the mixture was allowed to stand at 37° C. for 20 minutes. To this, 0.5 mL of 0.8 mg/mL sodium hyaluronate solution (from rooster comb) was added and reacted at 37° C. for 40 minutes. Thereafter, 0.2 mL of 0.4 mol/L sodium hydroxide was added to stop the reaction, and after cooling, 0.2 mL of boric acid solution was added to each reaction solution and boiled for 3 minutes. After cooling with ice, 6 mL of p-DABA reagent was added and reacted at 37° C. for 20 minutes. After that, absorbance at a wavelength of 585 nm was measured.

In addition, as a blank, the same operation and absorbance measurement were performed in the case where no enzyme solution was added. Furthermore, as a control, the same operation and measurement were performed using 0.1 mol/L acetate buffer (pH 3.5) to which no sample was added.
From the obtained results, the hyaluronidase activity inhibition rate (%) was calculated according to the following formula.
Hyaluronidase activity inhibition rate (%) = {1-(A-B) / (C-D)} × 100
Each term in the formula represents the following.
A: Absorbance at wavelength 585 nm with test sample added/enzyme added B: Absorbance at wavelength 585 nm with test sample added/enzyme added C: Absorbance at wavelength 585 nm with sample not added/enzyme added D: Sample not added/enzyme Table 1 shows the absorbance results at a wavelength of 585 nm without addition.

As shown in Table 1, it was confirmed that both the lactic acid bacterium fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella fungus (Sample 2) had excellent hyaluronidase activity inhibitory action.

[Test Example 2] Tumor necrosis factor (TNF-α) production inhibitory action test The lactic acid bacteria fermented product from the Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product from the yeast extract of Tremella tremosa obtained in Production Example 2 (Sample 2) was tested for TNF-α production inhibitory action as follows.

After culturing mouse macrophage cells (RAW264.7) using 10% FBS-containing Dulbecco's MEM medium, the cells were collected with a cell scraper. After diluting the collected cells with the above medium to a cell density of 1.0×10 ⁶ cells/mL, 100 μL of each well was seeded in a 96-well microplate and cultured for 4 hours.

After the culture was completed, the medium was removed, and 100 μL of test samples (Samples 1 and 2, see Table 2 below for sample concentrations) dissolved in Dulbecco's MEM medium containing 10% FBS with a final concentration of 0.5% DMSO was added to each well. 100 μL of lipopolysaccharide (LPS) (DIFCO, E. coli 0111; B4) dissolved in Dulbecco's MEM containing 10% FBS at a final concentration of 1 μg/mL was added and cultured for 24 hours. As a control, Dulbecco's MEM medium containing 10% FBS containing a final concentration of 0.5% DMSO to which no sample was added was used in the same manner. After completion of the culture, the amount of TNF-α in the culture supernatant of each well was measured using the sandwich ELISA method. From the obtained results, the TNF-α production inhibition rate (%) was calculated by the following formula.

TNF-α production suppression rate (%) = {(B-A) / B} × 100
Each term in the formula represents the following.
A: Amount of TNF-α with addition of test sample B: Amount of TNF-α with no addition of sample Table 2 shows the results.

As shown in Table 2, it was confirmed that both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) had an excellent TNF-α production inhibitory effect.

[Test Example 3] Elastin production promoting effect test The lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2) were treated as follows. Elastin production promoting action was tested.

Normal human skin fibroblasts (NB1RGB) were cultured using 10% FBS-containing Dulbecco's MEM medium, and then the cells were collected by trypsinization. After diluting the recovered cells with the above medium to a cell density of 2.2×10 ⁵ cells/mL, 100 μL of each well was seeded in a 96-well microplate and cultured overnight.

After the culture was completed, the medium was removed, and 150 μL of test samples dissolved in 0.25% FBS-containing Dulbecco's MEM medium (Samples 1 and 2, see Table 3 below for sample concentrations) were added to each well and cultured for 5 days. . As a control, Dulbecco's MEM medium containing 0.25% FBS to which no sample was added was used and cultured in the same manner. After the culture was completed, the supernatant was recovered, and the amount of elastin released in the culture supernatant was measured by ELISA. From the measurement results, the elastin production promotion rate (%) was calculated by the following formula.

Elastin production promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Amount of elastin with addition of test sample B: Amount of elastin with no addition of sample Table 3 shows the results.

As shown in Table 3, it was confirmed that both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) had an excellent elastin production promoting effect.

[Test Example 4] Epidermal hyaluronic acid production promotion test Regarding the lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2), the following was performed. The effect of promoting epidermal hyaluronic acid production was tested.

After culturing normal human neonatal epidermal keratinocytes (NHEK) using normal human epidermal keratinocyte growth medium (KGM), the cells were collected by trypsinization. After diluting the collected cells with the above medium to a cell density of 1×10 ⁵ cells/mL, 100 μL of each well was seeded in a 96-well plate and cultured for 24 hours.

After the culture was completed, the medium was removed, and 100 μL of test samples (Samples 1 and 2, see Table 4 below for sample concentrations) dissolved in KGM medium were added to each well and cultured for 7 days. As a control, a KGM medium containing no sample was cultured in the same manner. After culturing, the amount of hyaluronic acid in the medium of each well was measured by the sandwich method using hyaluronic acid binding protein (HABP). From the measurement results, the epidermal hyaluronic acid production promotion rate (%) was calculated by the following formula.

Epidermal hyaluronic acid production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Amount of hyaluronic acid with addition of test sample B: Amount of hyaluronic acid with no addition of sample Table 4 shows the results.

As shown in Table 4, both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) were found to have excellent epidermal hyaluronic acid production-promoting action.

[Test Example 5] Type IV Collagen Production Promotion Effect Test The fermented product of white fungus extract yeast (Sample 2) obtained in Production Example 2 was tested for type IV collagen production promotion effect in the following manner.

Normal human skin fibroblasts (NB1RGB) were cultured using 10% FBS-containing Dulbecco's MEM medium, and then the cells were collected by trypsinization. After diluting the recovered cells with the above medium to a cell density of 1.6×10 ⁵ cells/mL, 100 μL of each well was seeded in a 96-well microplate and cultured overnight.

After the culture was completed, the medium was removed, and 150 μL of test samples dissolved in 0.25% FBS-containing Dulbecco's MEM medium (Samples 1 and 2, see Table 5 below for sample concentrations) were added to each well and cultured for 3 days. bottom. As a control, Dulbecco's MEM medium containing 0.25% FBS to which no sample was added was used and cultured in the same manner. After culturing, the amount of type IV collagen in the medium of each well was measured by ELISA. From the measurement results, the promotion rate (%) of type IV collagen production was calculated according to the following formula.

Promotion rate of type IV collagen production (%) = A/B x 100
Each term in the formula represents the following.
A: Amount of type IV collagen with test sample added B: Amount of type IV collagen with no sample added Table 5 shows the results.

As shown in Table 5, the fermented yeast extract from Tremella tremella (Sample 2) had an excellent type IV collagen production promoting effect.

[Test Example 6] Epidermal Keratinocyte Proliferation Activation Test Regarding the lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2), the following Epidermal keratinocyte proliferation-promoting action was tested in this manner.

After culturing normal human neonatal epidermal keratinocytes (NHEK) using a normal human epidermal keratinocyte growth medium (KGM), the cells were collected by trypsinization. After diluting the collected cells with KGM medium to a cell density of 3.0×10 ⁴ cells/mL, 100 μL of each well was seeded on a collagen-coated 96-well plate and cultured overnight. After completion of the culture, 100 μL of test samples (Samples 1 and 2, see Table 6 below for sample concentrations) dissolved in KGM medium were added to each well and cultured for 3 days. As a control, KMG medium containing no sample was cultured in the same manner.

Epidermal keratinocyte proliferation-promoting activity was measured using the MTT assay method. That is, after culturing for 3 days, the medium was removed, and 100 μL of MTT dissolved in PBS(−) buffer at a final concentration of 0.4 mg/mL was added to each well. After culturing for 2 hours, intracellularly produced blue formazan was extracted with 100 μL of 2-propanol. After extraction, absorbance was measured at a wavelength of 570 nm. At the same time, absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was taken as the amount of blue formazan produced. From the obtained results, the epidermal keratinocyte proliferation promotion rate (%) was calculated by the following formula.

Epidermal keratinocyte proliferation promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Amount of blue formazan produced with addition of test sample B: Amount of blue formazan produced with no addition of sample Table 6 shows the results.

As shown in Table 6, it was confirmed that both the lactic acid bacteria fermented product of Tremella fungus (Sample 1) and the fermented product of yeast extract of Tremella tremella (Sample 2) had an excellent epidermal keratinocyte proliferation-promoting effect.

[Test Example 7] Transglutaminase-1 (TG-1) production promoting effect test The lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella japonicum extract obtained in Production Example 2 (Sample 2 ) was tested for TG-1 production promoting effect as follows.

After culturing normal human neonatal epidermal keratinocytes (NHEK) using a normal human epidermal keratinocyte growth medium (KGM), the cells were collected by trypsinization. After diluting the collected cells with the above medium to a cell density of 1×10 ⁵ cells/mL, 100 μL of each well was seeded in a 96-well plate and cultured for 2 days.

After completion of the culture, 100 μL of test samples (Samples 1 and 2, see Table 7 below for sample concentrations) dissolved in KGM medium were added to each well and cultured for 24 hours. As a control, a KGM medium containing no sample was cultured in the same manner. After culturing, the medium was removed, the cells were fixed on a plate, and the amount of transglutaminase-1 expressed on the cell surface was measured by ELISA using a monoclonal anti-human transglutaminase-1 antibody (manufactured by Biomedical Technologies Inc.). Measured by From the obtained measurement results, the promotion rate (%) of transglutaminase-1 production was calculated according to the following formula.

Transglutaminase-1 production promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: amount of transglutaminase-1 with addition of test sample B: amount of transglutaminase-1 with no addition of sample Table 7 shows the results.

As shown in Table 7, both the lactic acid bacteria fermented product of Tremella fungus (Sample 1) and the fermented product of yeast extract of Tremella tremella (Sample 2) were found to have an excellent TG-1 production promoting effect.

[Test Example 8] Profilaggrin mRNA expression promoting effect test The lactic acid bacterium fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2) were tested as follows. Then, profilaggrin mRNA expression promoting action was tested.

Normal human neonatal epidermal keratinocytes (NHEK) were pre-cultured using normal human epidermal keratinocyte growth medium (KGM), and the cells were collected by trypsinization. After diluting the collected cells with the above medium to a cell density of 15×10 ⁴ cells/mL, 2 mL of each was seeded in a 35 mm petri dish (30×10 ⁴ cells/dish) and cultured overnight.

After culturing, the medium was removed and the test samples (samples 1 and 2, sample See Table 8 below for the concentration) was added to each petri dish by 2 mL, and cultured for 24 hours under conditions of 37° C. and 5% CO ₂ . As a control, a KBM medium to which no sample was added was used and cultured in the same manner. After culturing, the medium was removed, total RNA was extracted with ISOGEN II (manufactured by Nippon Gene, Cat. No. 311-07361), and the amount of each RNA was measured with a spectrophotometer so as to reach 200 ng/μL. Total RNA was prepared at .

Using this total RNA as a template, the mRNA expression levels of profilaggrin and GAPDH as an internal standard were measured. Detection was performed by real-time 2-Step RT-PCR reaction using a real-time PCR device Smart Cycler (manufactured by Cepheid) and TaKaRa SYBR Prime Script RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio Inc., code No. RR063A). . The expression level of profilaggrin mRNA was corrected with the value of GAPDH based on total RNA preparations prepared from cells cultured with "test sample added" and "sample not added". From the obtained values, profilaggrin mRNA expression promotion rate (%) was calculated by the following formula.

Profilaggrin mRNA expression promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Correction value with addition of test sample B: Correction value with no addition of sample Table 8 shows the results.

As shown in Table 8, both the fermented product of lactic acid bacteria extracted from Tremella fungus (Sample 1) and the fermented product of yeast extract from Tremella Tremella (Sample 2) had an excellent profilaggrin mRNA expression promoting effect.

[Test Example 9] Serine palmitoyltransferase (SPT) mRNA expression promoting effect test Regarding the lactic acid bacteria fermented product of Tremella lucidum extract obtained in Production Example 1 (Sample 1) and the fermented product of yeast extract of Tremella tremosa obtained in Production Example 2 (Sample 2) , the SPT mRNA expression promoting effect was tested as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were pre-cultured using normal human epidermal keratinocyte growth medium (KGM), and the cells were collected by trypsinization. After diluting the collected cells with KGM medium to a cell density of 15 × 10 ⁴ cells / mL, seeding 2 mL each in a 35 mm petri dish (30 × 10 ⁴ cells / petri dish), 37 ° C., 5% CO ₂ conditioned overnight. After culturing, the medium was replaced with a normal human epidermal keratinocyte basal medium (KBM, the above KGM medium without addition of growth factors (hEGF, BPE, insulin)), and the cells were further cultured for 24 hours.

After culturing for 24 hours, the medium was removed, and 2 mL of test samples dissolved in KBM medium (Samples 1 and 2, see Table 9 below for sample concentrations) were added to each petri dish and incubated at 37°C and 5% CO ₂ conditions. It was cultured for 24 hours in the bottom. As a control, a KBM medium to which no sample was added was used and cultured in the same manner. After culturing, the medium was removed, total RNA was extracted with ISOGEN II (manufactured by Nippon Gene, Cat. No. 311-07361), and the amount of each RNA was measured with a spectrophotometer so as to reach 200 ng/μL. Total RNA was prepared at .

Using this total RNA as a template, the expression level of mRNA was measured for SPT and GAPDH as an internal standard. Detection was performed by real-time 2-Step RT-PCR reaction using a real-time PCR device Smart Cycler (manufactured by Cepheid) and TaKaRa SYBR Prime Script RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio, code No. RR063A). The expression level of SPT was corrected with the value of GAPDH based on total RNA preparations prepared from cells cultured with "test sample added" and "sample not added". From the obtained values, the SPT mRNA expression promotion rate (%) was calculated according to the following formula.

SPT mRNA expression promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Correction value with addition of test sample B: Correction value with no addition of sample Table 9 shows the results.

As shown in Table 9, it was confirmed that both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella tremella (Sample 2) had an excellent SPT mRNA expression promoting effect.

[Test Example 10] Aquaporin 3 (AQP3) mRNA expression promoting effect test Regarding the lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2), AQP3 mRNA expression promoting action was tested as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were pre-cultured using normal human epidermal keratinocyte growth medium (KGM), and the cells were collected by trypsinization. After diluting the collected cells with KGM medium to a cell density of 15 × 10 ⁴ cells / mL, seeding 2 mL each in a 35 mm petri dish (30 × 10 ⁴ cells / petri dish), 37 ° C., 5% CO ₂ conditioned overnight. After culturing, the medium was replaced with a normal human epidermal keratinocyte basal medium (KBM, the above KGM medium without addition of growth factors (hEGF, BPE, insulin)), and the cells were further cultured for 24 hours.

After culturing for 24 hours, the medium was removed, and 2 mL of test samples (Samples 1 and 2, see Table 10 below for sample concentrations) dissolved in KBM medium were added to each petri dish, and the conditions were 37°C and 5% CO2 _. It was cultured for 24 hours in the bottom. As a control, a KBM medium to which no sample was added was used and cultured in the same manner. After culturing, the medium was removed, total RNA was extracted with ISOGEN II (manufactured by Nippon Gene, Cat. No. 311-07361), and the amount of each RNA was measured with a spectrophotometer so as to reach 200 ng/μL. Total RNA was prepared at .

Using this total RNA as a template, the mRNA expression levels of AQP3 and GAPDH as an internal standard were measured. Detection was performed by real-time 2-Step RT-PCR reaction using a real-time PCR device Smart Cycler (manufactured by Cepheid) and TaKaRa SYBR Prime Script RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio Inc., code No. RR063A). . The expression level of AQP3 mRNA was corrected with the value of GAPDH based on total RNA preparations prepared from cells cultured with "test sample added" and "sample not added". From the obtained values, the AQP3 mRNA expression promotion rate (%) was calculated according to the following formula.

AQP3 mRNA expression promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Correction value with addition of test sample B: Correction value with no addition of sample Table 10 shows the results.

As shown in Table 10, it was confirmed that both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) had an excellent AQP3 mRNA expression promoting effect.

[Test Example 11] Hyaluronic acid synthase 3 (HAS3) mRNA expression promoting action test The lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of Tremella fungus extract obtained in Production Example 2 (Sample 2 ) was tested for the HAS3 mRNA expression promoting effect as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were pre-cultured using normal human epidermal keratinocyte growth medium (KGM), and the cells were collected by trypsinization. After diluting the collected cells with the above medium to a cell density of 15×10 ⁴ cells/mL, seeding 2 mL each on a 35 mm petri dish (30×10 ⁴ cells/dish), 37° C., 5% CO ₂ conditioned overnight. After culturing, the medium was replaced with a normal human epidermal keratinocyte basal medium (KBM, the above KGM medium without addition of growth factors (hEGF, BPE, insulin)), and the cells were further cultured for 24 hours.

After culturing for 24 hours, the medium was removed, and 2 mL of test samples dissolved in KBM medium (samples 1 and 2, see Table 11 below for sample concentrations) were added to each petri dish, and the conditions were 37°C and 5% _CO2. It was cultured for 24 hours in the bottom. As a control, a KBM medium to which no sample was added was used and cultured in the same manner. After culturing, the medium was removed, total RNA was extracted with ISOGEN II (manufactured by Nippon Gene, Cat. No. 311-07361), and the amount of each RNA was measured with a spectrophotometer so as to reach 200 ng/μL. Total RNA was prepared at .

Using this total RNA as a template, expression levels of mRNA were measured for HAS3 and GAPDH as an internal standard. Detection was performed by real-time 2-Step RT-PCR reaction using a real-time PCR device Smart Cycler (manufactured by Cepheid) and TaKaRa SYBR Prime Script RT-PCR kit (Perfect Real Time) (manufactured by Takara Bio Inc., code No. RR063A). . The expression level of HAS3 mRNA was corrected with the value of GAPDH based on total RNA preparations prepared from cells cultured with "test sample added" and "sample not added". From the obtained values, the HAS3 mRNA expression promotion rate (%) was calculated according to the following formula.

HAS3 mRNA expression promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Correction value with addition of test sample B: Correction value with no addition of sample Table 11 shows the results.

As shown in Table 11, both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) had an excellent HAS3 mRNA expression promoting effect.

[Test Example 12] Glutathione production promoting effect test The white fungus extract lactic acid bacterium fermented product (sample 1) obtained in Production Example 1 and the white fungus extract yeast fermented product (sample 2) obtained in Production Example 2 were treated as follows. The glutathione production promoting effect on B16 melanoma cells was tested.

B16 melanoma cells were precultured using 10% FBS-containing Dulbecco's MEM medium, and then the cells were harvested by trypsinization. The collected cells were diluted with 10% FBS-containing Dulbecco's MEM medium to a cell density of 10×10 ⁴ cells/mL, then seeded in 48-well plates at 200 μL per well and cultured overnight.

After culturing, the medium was removed, and 200 μL of test samples (Samples 1 and 2, see Table 12 below for sample concentrations) dissolved in Dulbecco's MEM medium containing 1% FBS was added to each well and cultured for 24 hours. As a control, Dulbecco's MEM medium containing 1% FBS to which no sample was added was used and cultured in the same manner. After the culture was completed, the medium was removed from each well, washed with 400 μL of PBS(-) buffer, and the cells were lysed using 150 μL of M-PER (manufactured by PIERCE).

100 μL of this was used to quantify total glutathione. That is, 100 μL of cell extract dissolved in a 96-well plate, 50 μL of 0.1 mmol/L phosphate buffer, 25 μL of 2 mmol/L NADPH and 25 μL of glutathione reductase (final concentration 17.5 units/mL) were added and heated at 37° C. for 10 minutes. After that, 25 μL of 10 mM 5,5′-dithiobis(2-nitrobenzoic acid) was added, and the absorbance at a wavelength of 412 nm was measured up to 5 minutes later to obtain ΔOD/min. The total glutathione concentration was calculated based on a calibration curve prepared using oxidized glutathione (manufactured by Wako Pure Chemical Industries, Ltd.). After correcting the obtained value to the amount of glutathione per total protein amount, the glutathione production acceleration rate (%) was calculated by the following formula.

Glutathione production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Glutathione amount per total protein amount in cells added with test sample B: Glutathione amount per total protein amount in cells without sample addition (control)
Table 12 shows the results.

As shown in Table 12, both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) were confirmed to have an excellent glutathione production-promoting effect on B16 melanoma cells.

[Test Example 13] Dermal Papilla Cell Proliferation Activation Test The lactic acid bacteria fermented product of Tremella fungus extract obtained in Production Example 1 (Sample 1) and the fermented product of yeast extract of Tremella Tremoli obtained in Production Example 2 (Sample 2) were tested as follows. The dermal papilla cell growth promoting action was tested.

Normal human hair dermal papilla cells (HFDPC, derived from male vertex) were cultured using a growth medium for dermal papilla cells (PCGM, manufactured by Toyobo Co., Ltd.) containing 1% FCS and growth additives, and then treated with trypsin. recovered. The collected cells were diluted with 10% FBS-containing DMEM medium to a cell density of 1.0×10 ⁴ cells/mL, and then seeded at 200 μL per well on a collagen-coated 96-well plate. cultured for days.

Thereafter, the medium was removed, and 200 μL of test samples (Samples 1 and 2, see Table 13 below for sample concentrations) dissolved in serum-free DMEM medium were added to each well, and the cells were further cultured for 4 days. As a control, cells were cultured in the same manner using a serum-free DMEM medium to which no sample was added. After completion of the culture, the dermal papilla cell proliferation-promoting action was measured by MTT assay. Specifically, the medium was removed, 200 μL of 0.4 mg/mL MTT prepared in serum-free DMEM medium was added, and the cells were further cultured for 2 hours, after which blue formazan produced in cells was extracted with 100 μL of 2-propanol. The absorbance of this extract was measured at 570 nm where the absorption maximum of blue formazan exists. At the same time, absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was taken as the amount of blue formazan produced. From the measurement results, the dermal papilla cell proliferation promotion rate (%) was calculated based on the following formula.

Dermal papilla cell proliferation promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Amount of blue formazan produced with test sample added B: Amount of blue formazan produced with no sample added Table 13 shows the results.

As shown in Table 13, both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) were found to have an excellent dermal papilla cell proliferation promoting effect.

[Test Example 14] Keratin-related protein 5.1 (KAP5.1) mRNA expression promoting action test Tremella fungus extract lactic acid bacterium fermented product obtained in Production Example 1 (Sample 1) and Tremella fungus extract yeast fermented product obtained in Production Example 2 (Sample 2) was tested for the KAP5.1 mRNA expression promoting effect as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were precultured using normal human epidermal keratinocyte growth medium (KGM) under conditions of 37°C and 5% CO ₂ , and the cells were collected by trypsinization. After diluting the collected cells with the above medium to a cell density of 15×10 ⁴ cells/mL, seeding 2 mL each on a 35 mm petri dish (30×10 ⁴ cells/dish), 37° C., 5% CO ₂ It was cultured under these conditions for 24 hours. After culturing, the medium was replaced with a normal human epidermal keratinocyte basal medium (KBM, the above KGM medium without addition of growth factors (hEGF, BPE, insulin)), and the cells were further cultured for 24 hours.

After culturing for 24 hours, the medium was removed, and 2 mL of test samples dissolved in KBM medium (Samples 1 and 2, see Table 14 below for sample concentrations) were added to each petri dish, and the conditions were 37°C and 5% _CO2. It was cultured for 24 hours in the bottom. As a control, a KBM medium to which no sample was added was used and cultured in the same manner. After culturing, the medium was removed, total RNA was extracted with ISOGEN II (manufactured by Nippon Gene, Cat. No. 311-07361), and the amount of each RNA was measured with a spectrophotometer so as to reach 200 ng/μL. Total RNA was prepared at .

Using this total RNA as a template, the expression levels of KAP5.1 and internal standard GAPDH mRNA were measured. Detection was performed by real-time 2-Step RT-PCR reaction using a real-time PCR device Smart Cycler (manufactured by Cepheid) and TaKaRa SYBR PrimeScript RT-PCR Kit (Perfect Real Time) (manufactured by Takara Bio Inc., code No. RR063A). . The expression level of KAP5.1 mRNA was corrected with the value of GAPDH based on total RNA preparations prepared from cells cultured with "test sample added" and "sample not added". From the obtained results, the KAP5.1 mRNA expression promotion rate (%) was calculated according to the following formula.

KAP5.1 mRNA expression promotion rate (%) = A/B x 100
Each term in the formula represents the following.
A: Correction value when test sample was added B: Correction value when no sample was added Table 14 shows the results.

As shown in Table 14, both the lactic acid bacteria fermented product of Tremella fungus extract (Sample 1) and the fermented product of yeast extract of Tremella Tremella (Sample 2) were found to have a KAP5.1 mRNA expression promoting effect.

[Formulation Example 1]
A milky lotion was prepared by a conventional method according to the following composition.
White fungus extract lactic acid fermented product (Production Example 1) 0.01 g
Jojoba oil 4.00g
1,3-butylene glycol 3.00 g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
2.00 g olive oil
2.00 g of squalane
Cetanol 2.00g
Glyceryl monostearate 2.00g
Polyoxyethylene sorbitan oleate (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10 g
Huangji extract 0.10g
Dipotassium glycyrrhizinate 0.10 g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Phellodendron bark extract 0.10g
Chamomile extract 0.10g
Perfume 0.05g
Remainder of purified water (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Tremella fungus extract yeast fermented product (manufacturing example 2) 0.05 g
Kujin extract 0.1g
Scutellaria root extract 0.1g
Liquid paraffin 5.0g
Bleached beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
1.0 g of stearic acid
Polyoxyethylene sorbitan oleate (20E.O.) 1.5g
Glyceryl monostearate 3.0g
Oil-soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
Methyl paraoxybenzoate 1.5g
Perfume 0.1g
Remainder of purified water (total amount is 100 g)

[Formulation Example 3]
A beauty essence having the following composition was prepared by a conventional method.
Tremella fungus extract yeast fermented product (manufacturing example 2) 0.01 g
Chamomile extract 0.1g
carrot extract 0.1g
xanthan gum 0.3g
0.1 g of hydroxyethyl cellulose
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
Dipotassium glycyrrhizinate 0.1g
2.0 g of glycerin
Potassium hydroxide 0.25g
Perfume 0.01g
Preservative (methyl paraoxybenzoate) 0.15g
2.0 g of ethanol
Remainder of purified water (total amount is 100 g)

[Formulation Example 4]
A hair tonic having the following composition was produced by a conventional method.
Tremella fungus extract lactic acid fermented product (Production Example 1) 0.4 g
Tocopherol acetate Appropriate amount Cephalatine 0.002g
Isopropylmethylphenol 0.1g
Sodium hyaluronate 0.15g
15.0 g of glycerin
Ethanol 15.0g
Perfume Appropriate amount Chelating agent (edetate sodium) Appropriate amount Preservative (hinokitiol) Appropriate amount Solubilizer (polyoxyethylene cetyl ether) Appropriate amount Purified water Balance

[Formulation Example 5]
A shampoo having the following composition was produced by a conventional method.
Tremella fungus extract yeast fermented product (manufacturing example 2) 0.5 g
Marjoram extract 1.0g
Plum fruit part extract 0.2g
Coconut fatty acid methyl taurate sodium 10.0 g
Coconut fatty acid amidopropyl betaine 10.0g
Sodium polyoxyethylene alkyl ether sulfate 20.0 g
Coconut fatty acid diethanolamide 4.0 g
Propylene glycol 2.0 g
Fragrance Appropriate amount Purified water Remainder (Total amount is 100g)

[Formulation Example 6]
A tablet having the following composition was produced by a conventional method.
Tremella fungus extract lactic acid fermented product (manufacturing example 1) 5.0 mg
Dolomite (containing 20% calcium and 10% magnesium) 83.4mg
Casein phosphopeptide 16.7mg
Vitamin C 33.4mg
Maltitol 136.8mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg

[Formulation Example 7]
An oral liquid preparation having the following composition was prepared by a conventional method.
<Composition in 1 ampoule (100 mL per bottle)>
Tremella fungus extract yeast fermented product (Production Example 2) 0.3% by mass
Sorbit 12.0% by mass
Sodium benzoate 0.1% by mass
Perfume 1.0% by mass
Calcium sulfate 0.5% by mass
Remainder of purified water (100% by mass)

The anti-inflammatory agent, anti-aging agent, whitening agent and hair restorer of the present invention are useful for preventing, treating or improving various skin inflammatory diseases; preventing, treating or improving skin aging symptoms; promoting healing of wounds or burns; prevention, treatment or improvement of sexual skin diseases; prevention, treatment or improvement of pigmentation such as blackening of the skin, age spots and freckles; prevention, treatment or improvement of alopecia, especially male pattern baldness; improvement; and so on.

Claims (4)

heat sterilizing and/or filtering a fermented product of a white fungus extract with Lactobacillus plantarum to obtain a white fungus extract fermented product,
A method for producing an anti-aging agent, characterized by using the obtained fermented extract of white fungus as an active ingredient. heat sterilizing and/or filtering a fermented product of a white fungus extract with Lactobacillus plantarum to obtain a white fungus extract fermented product,
A method for producing a whitening agent, characterized by using the obtained fermented extract of Tremella as an active ingredient. A method for producing a cosmetic used for anti-aging applications and/or whitening applications,
heat sterilizing and/or filtering a fermented product of a white fungus extract with Lactobacillus plantarum to obtain a white fungus extract fermented product,
A method for producing a cosmetic, comprising blending the obtained fermented extract of Tremella fungus. A method for producing a food and drink composition for use in anti-aging applications and/or whitening applications,
heat sterilizing and/or filtering a fermented product of a white fungus extract with Lactobacillus plantarum to obtain a white fungus extract fermented product,
A method for producing a food or drink composition, comprising blending the obtained fermented extract of Tremella fungus.