JP7262848B2 - Methods of preparing personal care products - Google Patents

Description

The present invention relates generally to methods for formulating substances for antimicrobial cleansing of the human body.

Various parts of the human body are home to many microbial species, primarily bacteria and fungi. The human body is protected by the outermost layer of skin, which contains not only beneficial but also pathogenic microorganisms. Acne (such as caused by Cutibacterium acnes), rashes (usually caused by yeast), (caused by Streptococcus or Staphylococcus) if pathogenic organisms invade skin conditions such as cellulitis, leprosy (caused by Mycobacterium leprae), candidiasis (caused by the yeast Candida), ringworm (caused by Tinea spp.) It can also cause disability. Therefore, care must be taken to maintain personal hygiene, and particular attention to genital hygiene is usually of paramount importance.

Current formulations for washing or bathing the human body generally intentionally kill nearly all microorganisms present on the skin with 90-99% efficiency on contact (bactericidal or fungicidal), or Contains inactivating (bacteriostatic or fungistatic) antibacterial substances. However, these antimicrobial products do not have the ability to distinguish between beneficial and non-beneficial microorganisms for the human body. As such, abuse of such antimicrobial products, in turn, can cause discomfort and even skin damage due to the loss of beneficial microbes that act as a natural barrier against attack by pathogenic microbes. Beneficial skin microbes such as lactobacilli actually constitute the first line of defense in the human immune system.

The current market for personal care products would benefit from an antimicrobial formulation that could selectively inactivate or eliminate pathogenic microorganisms on human skin rather than beneficial lactic acid bacteria. .

Wood vinegar generally contains various chemical constituents such as acetic acid, methanol, esters, acetals, ketones, and formic acid, which have antimicrobial properties suitable for use in personal care products, as well as strong antioxidant properties. Contains more than one type of organic compound. However, wood vinegars derived from different wood plant species contain different compositions of such compounds and therefore each require different formulation strategies before being applied to achieve its selective antimicrobial properties. I need. In addition, a large amount of guaiacol, which causes an unpleasant pungent odor, was also detected in wood vinegar derived from various plant species. Therefore, it is a further advantage to utilize optimal concentrations of plant species-derived wood vinegar without the presence of guaiacol.

The present invention features wood vinegar (also known as wood distillate), which is an antimicrobial agent.

In particular, the present invention relates to the preparation of a personal care product comprising the steps of obtaining wood vinegar and adding the wood vinegar into the personal care product such that the wood vinegar comprises 18-22% of the total weight or volume of the personal care product. Regarding the method.

Wood vinegar, obtained by pyrolysis of Rhizophora apiculata, inactivates or kills microbes that cause skin and urinary tract infections, but contains substantial amounts of beneficial microbes that help defend against attack by pathogenic microbes. keep

Preferably, wood vinegar is obtained without guaiacol.

Preferably, the retained beneficial microorganisms are lactic acid bacteria.

Preferably, wood vinegar makes up 20% of the total weight or volume of the personal care product.

Further, in the proposed method, wood vinegar is allowed to age for at least three months before being refined and added into personal care products. Wood vinegar is then purified by filtration or distillation to remove impurities.

The parts of Futaba mangrove used for pyrolysis include bark, stems, branches, roots, leaves or any combination thereof.

Refined wood vinegar contains, but is not limited to, syringol, benzoic acid, maltol, catechol and vanillin.

In some embodiments, personal care products include, for example, feminine genital washes, shower gels, soaps, facial cleansers, cosmetics, perfumes, body lotions, hair shampoos and conditioners, hair styling products, ointments, disinfectants, and other skin care products. and oral care products.

Antidegradation stabilizers are preferably added in personal care products.

Preferably, surfactants or detergents are added in personal care products that reduce the surface tension of liquid-liquid or liquid-solid interfaces.

Gelling agents and/or emulsifying agents are preferably added in personal care products.

In another embodiment, the present invention describes a personal care product that includes refined wood vinegar incorporated into the personal care product to account for 18-22% of the total weight or volume of the personal care product.

Wood vinegar is obtained by pyrolysis of Futaba mangrove and inactivates or kills the microbes that cause skin and urinary tract infections, but retains significant amounts of beneficial microbes.

The present invention comprises the features and combinations of parts hereinafter fully described and illustrated in the accompanying drawings without departing from the scope of the invention or sacrificing any of its advantages. It should be understood that various changes in detail are possible in scope.

In order to further clarify various aspects of some embodiments of the invention, the invention will now be described in more detail based on specific embodiments illustrated in the accompanying drawings. It is appreciated that these drawings depict only typical embodiments of the invention and are therefore not to be considered limiting of its scope. The invention will be described and explained with additional specificity and detail through the accompanying drawings, in which: FIG.
FIG. 1 shows the process flow from pyrolysis of the mangrove tree and leaves to condensation of the smoke into wood vinegar, and then the wood vinegar is purified and added into personal care products. FIG. 2 is a dot plot histogram showing the antibacterial activity (minimum inhibitory concentration and minimum bactericidal concentration) of test extracts against 28 microbial strains. Figure 3 shows 20 against E. coli ATCC25922, Staphylococcus aureus LCUM0005, Candida albicans ATCC90028, Lactobacillus pentosus LCUM1001 measured in percent reduction. Fig. 10 is a bar graph showing the reduction effect of percent concentrations of pyroligneous extract (all data points with negative percent measurements were recorded as zero percent reduction).

The general principles of the present invention relate to methods of making wood vinegar, an antibacterial extract, and incorporating it into personal care product formulations.

Referring to FIG. 1, wood vinegar (203, 301) is obtained from pyrolysis (100) of mangrove plant Futaba mangrove (101) and leaves (102) (also known as mangrove oil (bakau minyak)). . Pyrolysis (100) is heating to an elevated temperature above the ceiling temperature of the polymer (the temperature at which the polymer tends to revert to monomer) and can be in the form of dry distillation, hydrothermal pyrolysis or vacuum pyrolysis. In the absence of air and water, carbonization is carried out. Hydrous pyrolysis takes place in the presence of superheated water or steam, whereas vacuum pyrolysis takes place in a closed vacuum environment.

The pyrolysis (100) process produces smoke (103) and charred solids called char (104). The smoke (103) is condensed (200) in a condenser where cooling water is passed into one end of the condenser and out the other end to cool the smoke (103). . Excess unwanted gas (201) is released and condensate (202) is collected at the bottom of the condenser. The condensate (202) is further separated into freshly extracted wood vinegar (203), bio-oil and bitumen. Freshly extracted wood vinegar (203) is aged for three months or more before purification (300) by filtration or distillation to remove impurities.

The purified wood vinegar (301) is added into the personal care product (302) at a final concentration of 18-22%, preferably 20% of the total weight or volume of the personal care product (302). This suggests that the use of a product containing 20% purified pyroligneous acid (301) from Futaba mangrove (101) for 3 minutes has been associated with protection of human digestive, urinary and genital tracts such as Lactobacillus. It works efficiently and selectively against common skin bacteria, especially enterobacteria and staphylococci, which can be harmful to humans and cause bad body odor, but not against lactobacilli ( inactivating or killing).

The use of pyroligneous acid (203, 301) derived from Futaba mangrove (101) is proposed to replace common antimicrobial agents such as triclosan, triclocarban, benzalkonium chloride, benzethonium chloride, chloroxylenol.

In Example 1, the main compounds in wood vinegar (203, 301) are described.

Each of the various compounds in wood vinegar (203, 301) have different microbial inactivation mechanisms and efficiencies. Therefore, the ideal concentration of wood vinegar (203, 301) applied to selectively inactivate non-beneficial and harmful skin microorganisms while retaining beneficial microorganisms was optimized.

Based on specific examples (Examples 2-4) demonstrating efficacy studies conducted against 27 strains of pathogenic microorganisms (various species) and 1 strain of beneficial bacteria, the antimicrobial efficacy and Selection effects are further discussed.

Example 1
The major active antimicrobial compound in wood vinegar (203, 301) derived from the pyrolysis (100) of Futaba mangrove (101) is the syringol (International Union of Pure and Applied Chemistry (IUPAC) chemical Name: 2,6-dimethoxyphenol). Benzoic acid (constituting 4.8468%), maltol (IUPAC chemical name: 3-hydroxy-2-methylpyran-4-one, constituting 4.1448%), catechol (IUPAC chemical name: benzene-1,2-diol , 2.7173%), vanillin (IUPAC chemical name: 4-hydroxy-3-methoxybenzaldehyde, 1.3902%) are also present in wood vinegar (203, 301) and their antibacterial Contributes to activity. All of these compounds have been reported to be commonly present in wood vinegar from other species. However, a large amount of guaiacol, which causes an unpleasant pungent odor, was also detected in wood vinegar derived from other plant species. Guaiacol was not detected in the wood vinegar derived from Futaba mangrove. In fact, syringol and its derivatives accounted for about 30% of the chemical constituents detected by gas chromatography-mass spectrometry (GCMS) analysis, and were found to be the major constituents of pyroligneous acid derived from Futaba mangrove. Therefore, the aroma of wood vinegar derived from Futaba mangrove has a strong woody and smoky flavor provided by syringol.

Table 1 shows the library/ID of major compounds in wood vinegar from Futaba mangrove (101), their corresponding Chemical Abstract Service (CAS) registry numbers, and concentrations (R1) detected by GCMS. .

Example 2
The antibacterial activity of wood vinegar (as described in Table 2) is against E. coli, Enterobacter spp., Enterococcus faecalis, Enterococcus faecium, Acinetobacter spp., Green Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Bacillus cereus, Candida A wide range of bacterial species, including Candida glabrata, Candida tropicalis, Candida albicans, Candida kefyr, Candida parapsilosis, and Lactobacillus pentosus and fungal species and strains.

Example 3
The antibacterial activity of the wood vinegar extract, measured in terms of minimum inhibitory concentration and minimum bactericidal concentration, was measured against 28 microbial strains: Enteroinvasive Escherichia coli (EIEC) LCUM0013, Enterohemorrhagic Escherichia coli (EHEC) LC0014, Enteropathogenic Escherichia coli (EPEC) LCUM0015, Enterobacter sp. LCUM0105, Enterococcus faecalis ATCC33186, Enterococcus faecium ATCC700221, Acinetobacter sp. , Pseudomonas aeruginosa LCUM0112, Proteus mirabilis ATCC7002, Staphylococcus aureus LCUM0005, Staphylococcus epidermidis ATCC14990, Staphylococcus epidermidis ATCC35984, Staphylococcus saprophyticus ATCC15305, Bacillus cereus LCUM0001, Bacillus cereus LCUM0002, Candida glabrata RJ4 , Candida tropicalis RJ8, Candida albicans ATCC90028, Candida albicans ATCC90029, Candida kefir ATCC204093, Candida parapsilosis ATC90018 and Lactobacillus pentosus LCUM1001.

Referring to Figure 2 , the bactericidal effect generally occurred at extract concentrations higher than those required to achieve an inhibitory effect. Above 20% concentration, all tested strains were killed except for the beneficial bacterial strain Lactobacillus pentosus LCUM1001.

Example 4
E. coli ATCC25922, Staphylococcus aureus LCUM0005, Candida albicans ATCC90028 and Lactobacillus pentosus LCUM1001 by 20% concentration of wood vinegar extract reduced reduction rate (%) after exposure to wood vinegar for 1, 3 and 5 minutes ) (as shown in FIG. 2). A 3-minute exposure to wood vinegar produced the highest average reduction of harmful microorganisms (E. coli ATCC25922, Staphylococcus aureus LCUM0005 and Candida albicans ATCC90028) and a much lower reduction of beneficial bacteria (Lactobacillus pentosus). rice field. Its optimal concentration of wood vinegar selectively kills 99.99% of Gram-negative enteric bacteria, 99.40% of Gram-positive staphylococci and 73.47% of Candida, all of which cause skin and urinary tract infections. deactivated. However, the product had no effect on lactic acid bacteria as only 26.03% of the lactic acid bacteria were inactivated during 3 minutes of contact with the product. Other than the species investigated here, beneficial lactic acid bacteria that can be stored include L. acidophilus, L. brevis, L. casei, Lactobacillus casei, L. Bacillus fermentum, L. paraplantarum, L. plantarum and many others, plus others such as Bifidobacterium bifidum Also included are the common probiotic bacteria genera of

Examples of personal care products include genital washes, shower gels, soaps, facial cleansers, cosmetics, perfumes, body lotions, hair shampoos and conditioners, hair products, ointments, disinfectants, or other skin and oral care products. is mentioned.

Methods of preparing such personal care products may include blending other compounds in addition to wood vinegar to enhance the characteristics of the personal care products. Among the many compounds are those that act as surfactants, such as:
Anionic surfactants classified as sulfates, sulfonates, phosphates, carboxylates: famous alkyl sulfates such as ammonium lauryl sulfate, sodium lauryl sulfate, sodium dodecyl sulfate and their related sodium laureth sulfate, Alkyl carboxylates (soaps), e.g. sodium stearate, sodium lauroyl sarcosinate, as a more specialized class of carboxylic acids, carboxylic acid-based salts, such as alkyl ether sulfates such as sodium myreth sulfate, such as a fluorosurfactant;
Cationic surfactants classified as pH-dependent primary, secondary or tertiary amines, such as octenidine dihydrochloride: as cationic surfactants, also cetrimonium bromide (CTAB), such as permanently charged quaternary ammonium salts such as cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride or dioctadecyldimethylammonium bromide (DODAB);
CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), sultaines such as cocamidopropyl hydroxysultaine, cocamidopropyl betaine (having a carboxylic acid with ammonium) such as zwitterionic surfactants such as betaine;
There are nonionic surfactants classified as ethoxylates, fatty acid esters, alkylpolyglucosides, amine oxides, lauryldimethylamine oxide, sulfoxides, dimethylsulfoxides and phosphine oxides.

Antidegradation stabilizers (e.g. phosphites, alkylated phenols such as In addition to antioxidants, sequestering agents and chelating agents such as disodium ethylenediaminetetraacetic acid, ammonium polyaspartate, tetrasodium etidronate) can be added to personal care product formulations. Stabilizers also help maintain clarity, protect aromatic compounds, and prevent rancidity.

In addition, thickeners or gelling agents (e.g., polyethylene glycol, polyacrylic acid, vegetable gums) are used to promote intimate mixing and thickening of substances in personal care products to achieve stability and ease of application. , waxes) and emulsifiers (common examples being emulsifying waxes, cetearyl alcohol, polysorbate 20, ceteareth-20, etc.) can additionally be added to personal care product formulations, some of which also act as stabilizers. .

The invention may be embodied in other specific forms without departing from its essential characteristics. The described embodiments are to be considered in all respects only as illustrative and non-limiting. The scope of the invention is, therefore, indicated by the appended claims rather than by the foregoing description. All changes that come within the meaning and range of equivalency of the claims are to be embraced within their scope.

Claims (11)

a process of obtaining wood vinegar;
adding said wood vinegar into said personal care product such that said wood vinegar comprises 18-22% of the total weight or volume of said personal care product;
The wood vinegar is obtained by condensing smoke generated by pyrolysis of Futaba mangrove into a condensate and separating and purifying wood vinegar from the condensate,
The process of pyrolysis produces smoke and char, and the smoke is condensed in a condenser in which cooling water is passed through one end of the condenser to cool the smoke. out the other end to release excess unwanted gas, said condensate being collected at the bottom of said condenser,
The condensate is further separated into the freshly extracted wood vinegar, bio-oil and bitumen, and after aging the freshly extracted wood vinegar for at least 3 months, the wood vinegar is cleaned of impurities. purified by filtration or distillation for
The wood vinegar, which contains syringol, benzoic acid, maltol, catechol, and vanillin, inactivates or kills microbes that cause skin and urinary tract infections, but retains significant amounts of beneficial microbes. Methods of preparing personal care products. 2. The method of preparing a personal care product according to claim 1, wherein said wood vinegar is obtained without guaiacol. 2. The method of preparing a personal care product of claim 1, wherein said substantial amount of beneficial microorganisms helps protect against attack by pathogenic microorganisms. 4. A method for preparing a personal care product according to claim 1 or 3, wherein said retained beneficial microorganisms are lactic acid bacteria. 2. The method of preparing a personal care product according to claim 1, wherein the wood vinegar comprises 20% of the total weight or volume of the personal care product . 2. The method of preparing a personal care product according to claim 1, wherein the parts of Futaba mangrove used for pyrolysis are bark, stems, branches, roots, leaves or any combination thereof. 2. The personal care product of claim 1, wherein the personal care product is a feminine wash, shower gel, soap, facial cleanser, cosmetic, perfume, hair product, ointment, antiseptic, or skin and oral care product. Method of preparation. 2. The method of preparing a personal care product according to claim 1, wherein an anti-degradation stabilizer is added into said personal care product. 2. The method of preparing a personal care product according to claim 1, wherein a surfactant or detergent is added in the personal care product to reduce the surface tension of the liquid-liquid or liquid-solid interface. 2. The method of preparing a personal care product according to claim 1, wherein a gelling agent is added into said personal care product. 2. The method of preparing a personal care product according to Claim 1, wherein an emulsifier is added into the personal care product .

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