JP7291369B2 - Visceral fat reducing agent and food composition for reducing visceral fat - Google Patents

Description

NPMD NITE BP-02800

TECHNICAL FIELD The present invention relates to a visceral fat-reducing agent and a visceral fat-reducing food composition containing a grain culture of Cordyceps militaris mycelium as an active ingredient.

Since obesity is involved in the onset of various diseases, since April 2008, in Japan, specific medical examinations and specific health guidance (so-called "metabolism examinations") for measuring the waist circumference and the like have been implemented. In particular, visceral fat-type obesity is considered to be one of the causes of lifestyle-related diseases such as diabetes, arteriosclerosis, hyperlipidemia, hypertension, cardiovascular disease, and stroke. In some cases, it is important to carry out treatment to reduce visceral fat.

Treatment methods for visceral fat-type obesity mainly include diet therapy that restricts calorie intake and exercise therapy that aims to burn fat through exercise. Visceral fat-reducing agents are used to enhance the effects of these therapies. have been proposed. For example, Patent Document 1 discloses a visceral fat-reducing agent containing a horse chestnut bark extract as an active ingredient, and Patent Document 2 discloses that an enzyme-treated product of squeezed lees of Satsuma mandarin orange has a visceral fat-reducing effect. It is described to have

On the other hand, the genus Cordyceps, which is called thoracic fungus, is a peculiar fungus that parasitizes the larvae of insects such as bat moths, cicadas, and silkworms and forms mushrooms, which are fruiting bodies. For example, Cordyceps sinensis is a type of parasitic fungus that parasitizes bat moth larvae, and is famous as a herbal medicine. Cordyceps militaris is a fungus parasitic on silkworm larvae.

Patent No. 4415063 Patent No. 5830214

As described above, in order to eliminate metabolic syndrome, it is necessary to develop and provide materials such as medicines and foods that can effectively and quickly reduce visceral fat, are highly safe, and can be obtained at low cost. is still expected.

On the other hand, the use of Cordyceps militaris, a fungus, as a treatment for visceral fat-type obesity or as a visceral fat-reducing agent has not been studied so far, and its efficacy has been completely unknown.

Therefore, the present invention has been made in view of the above points, and an object of the present invention is to provide a visceral fat-reducing agent and a visceral fat-reducing agent that are excellent in safety, can be produced at low cost, and exhibit a high visceral fat-reducing effect. It is to provide a food composition for

In order to solve the above problems, the visceral fat-reducing agent of the present invention contains a grain culture of Cordyceps militaris mycelium.

This cereal culture can be used as a visceral fat-reducing agent because it rapidly reduces the amount of visceral fat, as shown in the examples below. Furthermore, it is highly safe because it contains, as active ingredients, cultures of grains, which are food materials, and the mycelium of Chrysalis chrysalis, a herb that has been used as a crude drug in Chinese herbal medicine.

Moreover, it is also preferable that the grain in the visceral fat-reducing agent of the present invention is rice. This allows selection of suitable raw materials for the grain culture.

Moreover, it is also preferable that the above-mentioned pupa mushroom (Cordyceps militaris) is Cordyceps militaris KT16514 strain identified by accession number: NITE BP -02800. This selects suitable pupa strains that yield cereal cultures that are the active ingredient of the present invention.

Also, the food composition for reducing visceral fat of the present invention contains a grain culture of Cordyceps militaris mycelium. Since this grain culture rapidly reduces the amount of visceral fat, it can be used as a food composition for reducing visceral fat. Furthermore, since the active ingredient is a culture of grains, which is a food material and the mycelia of the thrombi fungus that has been used as a crude drug in Chinese herbal medicine, it is highly safe and can be easily taken orally as a food. can be done.

Also, the grain in the food composition for reducing visceral fat of the present invention is preferably rice. This allows selection of suitable raw materials for the grain culture.

Moreover, it is also preferable that the above-mentioned pupa mushroom (Cordyceps militaris) is Cordyceps militaris KT16514 strain identified by accession number: NITE BP -02800. This selects suitable pupa strains that yield cereal cultures that are the active ingredient of the present invention.

In addition, the method for producing a food composition for reducing visceral fat of the present invention is produced by inoculating a cereal raw material with Cordyceps militaris mycelium and culturing to obtain a cereal culture. Since it is obtained by culturing the pupa mycelium with grain raw materials, it is possible to produce it at a relatively low cost without the need for complicated processes.

According to the present invention, it is possible to provide a visceral fat-reducing agent and a visceral fat-reducing food composition having the following excellent effects.
(1) Excellent effect of reducing visceral fat.
(2) It is highly safe and can be taken orally because it contains, as active ingredients, a culture of the mycelium of pupa pupa, a fungus that has been used as a crude drug in Chinese herbal medicine, and cereals, which is a food material.
(3) Since it is produced by culturing the mycelium of pupa mushrooms with grain raw materials, it can be obtained simply and at low cost without the need for complicated processes such as chemical synthesis, extraction and purification.

1 is a flow chart that schematically illustrates a method for producing a cereal culture of Cordyceps militaris mycelium according to an embodiment of the present invention. (a) Graph showing changes in body weight of mice in the control group and group administered CM-R daily for 3 days, and (b) change in body weight of mice in the control group and group administered CM-R daily for 6 days, in Example 3. is a graph showing 10 is a graph showing changes in visceral fat mass of mice in the control group and each administration group in Example 4. FIG. 10 is a graph showing relative changes in visceral fat mass per 10 g body weight of mice in the control group and each administration group in Example 4. FIG.

First, referring to FIG. 1, a method for producing a grain culture of Cordyceps militaris mycelium, which is contained as an active ingredient in the visceral fat-reducing agent and visceral fat-reducing food composition of the present invention, will be described.

As shown in FIG. 1, the method for producing a grain culture of Cordyceps militaris mycelia according to the embodiment of the present invention includes step S0 of preparing grain raw materials, step S1 of adding water to the grain raw materials to absorb water, grain Step S2 of steaming and sterilizing the raw material, Step S3 of inoculating the steamed grain raw material with pupa mycelium, Step S4 of performing solid culture, Step S5 of obtaining a grain culture, and Processing the grain culture into a freeze-dried powder. It is roughly configured from step S6.

[Preparation of grain raw materials]
First, step S0 of preparing grain raw materials shown in FIG. 1 will be described. The cereal raw material in the present invention refers to a seed raw material mainly composed of starch, and specifically, seeds of gramineous, leguminous or other cereals are used. Plants belonging to the Gramineae family include barley such as rice, corn, wheat, barley, oat and rye, millet, millet, sorghum, barnyard millet and the like. Examples of leguminous plants include soybeans, adzuki beans, peas, peanuts, mung beans and the like, and examples of other millet plants include buckwheat. Among these, as the grain raw material according to the present invention, the seeds of plants belonging to the family Gramineae are preferable, and from the viewpoint of the effect of reducing visceral fat and the efficiency of cultivation, rice, barley, and wheat are more preferably used, and rice is particularly used. It is preferably used.

When rice is used as a cereal raw material, rice from which the husk has been removed, that is, unpolished rice is used from the viewpoint of culture efficiency in the culture step S4 described later. In addition, from the viewpoint of improving the culturing speed in the early stage of culturing, it is preferable to use polished rice, which is obtained by further polishing brown rice, in combination with brown rice. In polished rice, the starch is broken down into its constituent units, glucose, more quickly than in brown rice. Therefore, it is possible to activate the microorganisms that have just been inoculated into the raw rice material in the early stage of cultivation, and to improve the cultivation efficiency. If only polished rice is used as a cereal raw material, the nutrient components are removed too much, and the cultivation efficiency tends to decrease after the middle stage of cultivation. Therefore, it is preferable to use a combination of brown rice and polished rice. As for the mixing ratio of brown rice and polished rice, it is preferable to mix 1 to 19 parts by weight of brown rice with 1 part by weight of polished rice (that is, the weight ratio of brown rice/polished rice = 1 to 19). It is more preferable to blend 1 to 9 parts by weight of brown rice with respect to parts by weight (that is, the weight ratio of brown rice/polished rice=1 to 9). Polished rice also includes further polished rice such as germ rice and rinse-free rice. Brown rice and polished rice can be used in the form of rice grains as they are, but it is also possible to use powdered rice obtained by pulverizing at least a portion of the rice. In addition, the rice raw material can be blended with grain raw materials other than rice, and bran, glucose, inorganic salts, vitamins, etc. can be added as necessary.

[Water absorption treatment]
Next, the water absorption treatment step S1 will be described. In this step, water is added to the above grain raw material, or the grain raw material is immersed in water to absorb water. For example, when rice is used as a cereal raw material, the water absorption is adjusted so that the water content is 20 to 60%, preferably 30 to 45%. When soybeans are used as grain raw materials, the water content is adjusted to 30 to 70%, preferably 40 to 60%.

[Steaming process]
Next, the steaming treatment step S2 will be described. Using a high-pressure steam sterilizer or the like, steam is applied to the grain raw material that has been absorbed in the above-described process, and the raw material is steamed. The temperature and time of the steaming treatment are preferably 100 to 121° C. for about 15 minutes to 60 minutes, more preferably 15 minutes to 30 minutes, and particularly preferably 121° C. for about 20 minutes. By performing this step, various bacteria in the grain raw material are sterilized, and in the subsequent culture step, the grain raw material is decomposed, digested and absorbed by the pupa mycelium. In addition, the steaming process converts the starch in the grain raw material into gelatin and makes it easier to decompose, so that the pupal mushroom mycelium can be efficiently propagated in the grain raw material in the subsequent culture step. Grain raw materials that have been steamed are allowed to cool to about 30 to 40°C. This makes it possible to inoculate cereal raw materials with the pupa mycelium.

[Inoculation of pupa mycelium]
Next, the step S3 of inoculating the cereal raw material with the pupa mycelium will be described. In this step, the cereal raw material cooled through the steaming and sterilization steps described above is inoculated with Cordyceps militaris mycelium. Sanagitake is a type of ascomycete fungi belonging to the genus Chrysomeliaceae of the family Chrysomeliaceae, which parasitizes the pupae of silkworms and the like, and is a kind of ascomycete that is widely distributed in China, Korea, Japan, and the like. When inoculating the pupa mycelium, it is preferable to prepare a liquid spawn in advance and use it as a starter. The liquid spawn is not particularly limited, but can be prepared as follows as an example. 120 mL of a liquid medium for an inoculum containing 3% by weight of glucose, 2% by weight of red rice bran, 0.5% by weight of soybean flour, 0.5% by weight of yeast extract, and 0.2% by weight of sodium aspartate was added to 500 mL. Place in an Erlenmeyer flask, sterilize at 121° C. for 20 minutes, and allow to cool. Thereafter, a liquid spawn can be obtained by inoculating a 1 cm×1 cm mycelium disc of pupa mushroom and culturing at about 24° C. for about 7 days.

In the present invention, Cordyceps militaris KT16514 strain (accession number: NITE BP -02800) is preferably used as the pupa mushroom. This makes it possible to obtain a cereal culture that is excellent in reducing visceral fat. The Cordyceps militaris KT16514 strain is a mycelium of pupa mushroom that was purely isolated from the fruiting body found in the inner forest of Tochigi Prefecture. at a culture temperature of 23 to 27° C. and a culture period of 25 to 35 days. In addition, when the base sequence of the ITS-5.8S rDNA region of this Cordyceps militaris KT16514 strain was analyzed, a BLAST search against Apollon DB-FU revealed that it was identical to the Cordyceps militaris IFO30377 strain (Accession No. AB070375). It has been found to have 100% sex and 99.8% identity to Cordyceps militaris strain IFO9787 (Accession No. AB070374). Moreover, the result of BLAST search against the international nucleotide sequence database also revealed that the homology with Cordyceps militaris species was 99.6% or more. From these facts, the taxonomic group to which the Cordyceps militaris strain KT16514 belongs is Cordyceps militaris (L.) Fr. has been identified.

For the inoculation of the pupa mushroom mycelium, the liquid inoculum of pupa mushroom in an amount of 1 to 5% by weight based on the cereal material is uniformly dispersed and mixed with the cereal material. After inoculating the pupa mycelium into the cereal material, it is preferable to stir well to disperse the pupa mycelium throughout the cereal material.

[culture]
Next, the step S4 of culturing with the pupa mycelium inoculated into the grain raw material will be described. In this step, the pupa pupa mycelium is propagated on the cereal raw material inoculated with the pupa pupa mycelium. The culture temperature of the pupa mycelium is preferably 20 to 30°C, more preferably 23 to 25°C. The culture period is preferably about 2 weeks to 2 months, and 7 to 10 days after the inoculum is inoculated, a mixing operation is performed so that the mycelium spreads uniformly throughout, and the culture period is 21 days to 21 days. About 40 days is more preferable. Cultivation of the cereal raw material of the pupa mycelium is completed when a predetermined culture period has passed and the mycelium has spread sufficiently.

[Grain culture]
In the obtained grain culture S5 of the pupa mycelium, the pupa mycelium propagates over the entire grain raw material. When using this grain culture as a visceral fat-reducing agent and a food composition for reducing visceral fat, it is preferable to sterilize the entire grain culture. The sterilization treatment is carried out by a known method, for example, sterilization is performed by heating at 121° C. for 20 minutes using a high-pressure steam sterilizer or the like. Moreover, by performing this heat sterilization treatment, the cereal culture of the pupal mushroom mycelium is sterilized and at the same time heat-treated to obtain the hot water extraction effect.

[Lyophilized powder]
Next, the step S6 of processing the grain culture of the pupa mycelium into a freeze-dried powder will be described. The sterilized grain culture contains a certain amount of water, but the water is removed by freeze-drying to obtain a dried grain culture. A dried product may be obtained by natural drying, hot air drying, low-temperature vacuum drying, or the like. A freeze-dried powder that is easy to handle can be obtained by pulverizing the dried product of the obtained grain culture. It is also possible to make the obtained powder into granules or tablets. Even after heat sterilization, moisture removal by heating, or freezing and pulverization, the function of the pupa mycelium as a grain culture is effectively maintained. This cereal culture of pupa mycelium has a sufficient effect of reducing visceral fat even as it is. Therefore, complicated steps such as further extraction and separation and purification operations are unnecessary, and the finished product can be obtained simply and at low cost.

The visceral fat-reducing agent and visceral fat-reducing food composition of the present invention contain, as an active ingredient, the grain culture cultured with the above-described pupa mushroom mycelium, and are orally administered or ingested to reduce visceral fat. It has a rapid reducing action. The dosage of the visceral fat-reducing agent of the present invention varies depending on the target reduction amount or other therapy such as exercise or calorie restriction, administration method, age, etc., so it cannot be categorically defined, but it is usually a daily dose. The oral dose is about 10 to 1000 mg/kg body weight, preferably about 25 to 500 mg/kg body weight, more preferably about 50 to 300 mg/kg body weight as a grain culture of pupa mycelium, It may be administered in 1 to 3 divided doses from the start of administration.

The visceral fat-reducing agent of the present invention can be prepared in various forms by conventional methods. In this case, it can be formulated using additives that are generally accepted as pharmaceutical additives, such as carriers and excipients for formulations. In addition, in order to improve the bioavailability and stability of the grain culture of the pupa mycelium according to the present invention, drug delivery including formulation techniques such as microcapsules, micronization, clathration using cyclodextrin, etc. system can also be used.

Furthermore, the visceral fat-reducing agent can be used in the form of tablets, granules, capsules, internal liquid preparations, etc., and is preferably used in a form suitable for absorption from the gastrointestinal tract. Conventional formulation techniques can also be used to provide formulations in desired forms for reasons such as distribution and storage stability.

In addition, the food composition for reducing visceral fat of the present invention can be used in the form of supplements such as tablets, capsules, granules, and syrups, beverages, sweets such as candy, gum, and chocolate, bread, porridge, cereals, noodles, jelly, It can be used as a food composition in all forms such as soups, dairy products and seasonings. When used as a food composition in this way, it is possible to combine various other active ingredients, nutrients such as vitamins, minerals, amino acids, etc. within the range that does not affect the efficacy of the active ingredients of the present invention. . Foods developed from the food composition of the present invention include supplements, health foods, functional foods, foods for specified health uses, and the like. In addition, the daily intake of the food composition of the present invention is preferably about 10 to 1000 mg/kg body weight, and about 25 to 500 mg/kg body weight, as the grain culture of the above-mentioned pupa mushroom mycelium. is more preferable, and about 50 to 300 mg/kg body weight is more preferable, and it is preferable to divide this into 1 to 3 times.

Next, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by these Examples.

[Example 1]
1. Preparation of Liquid Inoculum of Cordyceps militaris 120 mL of the liquid inoculum medium shown in Table 1 was placed in a 500 mL Erlenmeyer flask and sterilized by heating at 121° C. for 20 minutes. After standing to cool, a 1 cm×1 cm storage seed (Cordyceps militaris strain KT16514) mycelium disc was inoculated and cultured at 24° C. for 7 days to obtain a liquid seed. This Cordyceps militaris KT16514 strain is a pupa mushroom strain purely isolated by the present inventor (Fumio Kobayashi), and has been deposited with the National Institute of Technology and Evaluation, Patent Microorganisms Depositary under the accession number: NITE BP -02800. . In addition, the ITS-5.8S rDNA region of this Cordyceps militaris KT16514 strain was subjected to nucleotide sequence analysis, and as a result, the belonging taxonomic group was Cordyceps militaris (L.) Fr. It has been confirmed that

[Example 2]
2. Preparation of rice culture of Cordyceps militaris mycelium In this example, a rice raw material obtained by mixing brown rice and polished rice was used as the grain of the culture raw material. The mixing ratio of the brown rice and the polished rice was 9:1 by weight. Since the moisture content of commercially available rice is about 12%, water was added to make the rice raw material absorb water so that the moisture content was about 40%. Specifically, 1000 g of raw material, which was made to absorb water by adding water at a rate of 400 mL per 1000 g of rice raw material, was weighed and filled in a heat-resistant culture bag. Thereafter, the raw rice material was steamed and sterilized by heating at 121° C. for 20 minutes. After the raw rice material was allowed to cool, the liquid inoculum of the Cordyceps militaris KT16514 strain obtained in Example 1 was aseptically mixed and inoculated into the raw rice material so as to be 3% by weight of the raw rice material. After solid culture at 24° C. for 30 days, extraction sterilization was performed by heating at 121° C. for 20 minutes, followed by freeze-drying and crushing into powder. Thus, a rice culture of the pupa mycelium of the present invention was obtained (hereinafter also referred to as "CM-R"). This rice culture was analyzed for nutritional labeling components, heavy metals and microorganisms. The results are shown in Table 2 below.

[Example 3]
3. Examination of safety by administration of rice culture of pupa mycelium In order to verify the safety of the rice culture of pupa mycelium (CM-R), the CM-R obtained in Example 2 was administered to mice every day, Body weight was measured and conditions such as organs were investigated. Specifically, the test was conducted as follows. Fifty 7-week-old ICR male mice (Japan SLC, Inc.) were obtained, acclimatized for about one week, and divided into 10 groups of 5 mice each as shown in Table 3 below. The CM-R obtained in Example 2 was adjusted to 25 mg, 50 mg, 100 mg and 200 mg/kg of mouse body weight for 8-week-old ICR male mice with distilled water. was orally administered to mice in each administration group using a gastric probe. The total amount administered was 0.2 mL/time, and the same amount of distilled water was administered to the control group. The administration period was once a day for 3 consecutive days and 6 consecutive days. During the administration period, the mice were fasted from 2:00 pm to 4:00 pm and weighed before administration. In addition, 24 hours after the final administration, euthanasia was performed by excessive inhalation of an inhalation anesthetic for animals (Isoflur: product of Zoetis Japan Co., Ltd.), and the appearance of the mice was examined. Subsequently, dissection was performed, and the position and shape of each organ, and the presence or absence of bleeding and adhesion were examined.

FIG. 2 shows changes in body weight of mice during oral administration of CM-R. FIG. 2(a) is a graph for the 3-day administration group, and FIG. 2(b) is a graph for the 6-day administration group. No significant increase or decrease in body weight was observed due to continuous administration for 3 to 6 days, and a spontaneous increase in body weight (1.8% to 4.7%) was observed in each administration group. Specifically, in the CM-R administration group for 3 days, the control group showed a weight gain of 1.8%, except for the 100 mg/kg administration group, and 4.1 in the 25 mg/kg to 200 mg/kg administration group. A body weight gain of ~4.7% was observed. In the CM-R administration group for 6 days, a 1.8% weight gain was observed in the control group, and 0.9 to 3.0% weight gain was observed in each of the 25 mg/kg to 200 mg/kg administration groups. . On the other hand, no abnormalities in motility, behavior and appetite were observed in mice in both the administration group and the control group during the administration period, and no abnormalities such as staining of body hair and diarrhea were observed even after daily administration. In addition, when dissection was performed and the position and shape of each organ and the presence or absence of bleeding and adhesion were examined, no abnormalities such as the position, shape, bleeding and adhesion of each organ were observed in both the administration group and the control group. I didn't.

From these results, no notable abnormalities were observed in 3-day administration and 6-day administration of 25 mg/kg, 50 mg/kg, 100 mg/kg and 200 mg/kg of CM-R. This confirmed that the rice culture of the pupa mycelium is highly safe.

[Example 4]
4. Reduction of visceral fat by rice culture of pupa mycelium Peri-testicular fat was collected, and the average value for each test group and control group was determined. Peri-testicular fat is a representative of intra-abdominal fat, and can be collected as an adipose tissue independent of other tissues. The results are shown in FIGS. 3 and 4. FIG.

The vertical axis of the graph in FIG. 3 is the measured adipose tissue weight (mg), and each bar in the bar graph indicates, from the left, the control group (0 mg/kg), the CM-R 25 mg/kg administration group, and the CM-R 50 mg/kg. A kg administration group, a CM-R 100 mg/kg administration group, and a CM-R 200 mg/kg administration group are shown. As shown in FIG. 3, the results showed that the visceral fat mass was reduced in the CM-R-administered group compared to the control group. Specifically, while the average peri-testicular fat weight in the control group was 805 mg, the average per-testicular fat weight in the test group to which CM-R was administered at 25 mg/kg was only 790 mg. , the mean value of the CM-R 50 mg/kg administration group decreased to 632 mg (78.5% of the control group weight), and the CM-R 100 mg/kg administration decreased to 698 mg (86% of the control group weight). 7%), and decreased to 564 mg in the CM-R 200 mg/kg administration group (reduced to 70.1% of the weight of the control group). Among them, the CM-R 200 mg/kg administration group showed a significant decrease (P<0.05). Thus, it was found that administration of CM-R, a rice culture of pupa mycelium, reduced visceral fat.

The graph in FIG. 4 shows the relative weight change per 10 g of mouse body weight in each test group and control group. Generally, the weight of the peri-testicular adipose tissue correlates with the age and weight of the mice. The vertical axis of the graph is the weight (mg) of testicular adipose tissue per 10 g of mouse body weight, and each bar in the bar graph indicates, from the left, the control group (0 mg/kg), the CM-R 25 mg/kg administration group, and the CM-R 50 mg. /kg administration group, CM-R 100 mg/kg administration group and CM-R 200 mg/kg administration group. According to the results, it was shown that the amount of visceral fat in the body weight was reduced in the CM-R-administered group as compared with the control group. Specifically, the average peri-testicular fat weight of the control group per 10 g of mouse body weight was 207 mg, while the average value of the test group administered 25 mg/kg of CM-R remained at 193 mg. , the mean value of the CM-R 50 mg/kg administration group decreased to 156 mg (75.4% of the weight of the control group), and that of the CM-R 100 mg/kg administration decreased to 179 mg (87% of the weight of the control group). 3%), and decreased to 143 mg in the CM-R 200 mg/kg administration group (reduced to 69.1% of the weight of the control group). Among them, the CM-R 50 mg/kg administration group and the CM-R 200 mg/kg administration group showed a significant decrease (P<0.05). From this result, it was found that administration of CM-R selectively reduced visceral fat rather than overall body weight. Thus, it was found that administration of CM-R, a rice culture of pupa mycelium, reduced visceral fat.

The present invention is not limited to the above-described embodiments or examples, and includes various design changes within the scope of the invention within the scope of the invention described in the claims. .

NITE B P-02800

Claims (4)

containing a solid rice culture of Cordyceps militaris mycelium ;
The visceral fat-reducing agent , wherein the rice comprises 1 to 19 parts by weight of brown rice per 1 part by weight of polished rice . 2. The visceral fat reducing agent according to claim 1 , wherein the pupa mushroom (Cordyceps militaris) is Cordyceps militaris KT16514 strain identified by accession number: NITE BP-02800. containing a solid rice culture of Cordyceps militaris mycelium ;
A food composition for reducing visceral fat , wherein the rice is a mixture of 1 to 19 parts by weight of brown rice per 1 part by weight of polished rice . The food composition for reducing visceral fat according to claim 3 , wherein the pupa mushroom (Cordyceps militaris) is Cordyceps militaris KT16514 strain identified by accession number: NITE BP-02800.

Images