JP7329474B2 - Anti-aging cosmetic composition - Google Patents

Description

The present invention relates to concentrated birch sap and anti-aging cosmetic compositions comprising concentrated birch sap.

Birch is a deciduous tree belonging to the Betulaceae family, and currently there are about 100 species in the world, which are mainly distributed in the northern temperate zone and the cool temperate zone. Among them, there are about 29 species in China, mainly distributed in the northeast, northwest and southwest. Birch trees grow mainly in remote mountainous areas with little human intervention and no industrial pollution. Birch sap (also called birch juice) is the fresh sap from the cut birch bark or pierced trunk. Birch sap is colorless or pale yellow with no sediment or impurities and has a faint birch fragrance. Birch sap contains numerous compounds such as sugars, amino acids, vitamins, biotin, cytokinins, trace mineral elements, aromatic oils, betulin and saponins, and has excellent anti-aging effects.

Aging of the skin means a reduction in the ability of the skin to protect and regulate the body, thus being unable to adapt to changes in the internal and external environment and exhibiting some changes in overall appearance such as skin color and condition. , resulting in reduced or lost skin elasticity, sagging skin, increased wrinkling, dry skin, thinning, easy scaling, itching, dull complexion, dullness, and concomitant dark spots and warts. As the population ages and living standards continue to improve, consumer awareness of anti-wrinkle continues to grow, and more and more consumers want to use cosmetics to maintain a youthful appearance. Statistics show that anti-aging cosmetics have become the fastest growing and most promising category in China's cosmetics market.

In one aspect, the present invention provides a concentrated birch sap having a degree of enrichment of 1.05-8 fold, preferably 1.1-4 fold, more preferably 1.2-2 fold. Concentrated birch sap has significant anti-aging effects.

The birch sap involved in the present invention is of the genus Betulaceae of the family Betulaceae, which includes four varieties: Betalaalba, Betula pubescens, Betula Pendula and Betula platyphylla. ula) obtained from Birch sap is obtained by artificially drilling holes in the base of birch tree trunks from the time the snow begins to melt until the trees begin to put out leaves. is high in birch sap. Birch sap is commercially available and used as is (in which case it is also called unconcentrated birch sap, or birch sap undiluted solution), for example from Daxinganling Chaoyue Wild Berry Development Co. , Ltd.

The concentrated birch sap used in the present invention is obtained by concentrating the commercially available birch sap stock product described above. Concentration methods such as thermal concentration, cryogenic and vacuum concentration, and membrane concentration are known in the art. In the present invention, concentration is preferably performed by a cryogenic freeze concentration or membrane concentration process. For example, a commercially available birch sap stock solution is introduced into a low temperature drying device, cooled to -40 to -70°C, and vacuumed to 0.1 to 30 Pa for the low temperature and vacuum concentration process, with various degrees of concentration. Obtain concentrated birch sap.

The present inventors have surprisingly found that concentrated birch sap has significantly better moisturizing and anti-aging effects compared to unconcentrated birch sap stock solution, demonstrating that type I, III, IV Promotes synthesis of type, VII, XVII collagen, elastin and laminin, reduces expression of matrix metalloproteinase MMP-1, promotes production of hyaluronic acid, structures of the epidermis, dermis, and dermal-epidermal junction enhance skin integrity and functionality, improve skin elasticity, reduce wrinkles while improving skin barrier, reducing transepidermal water loss, anti-inflammatory and soothing I discovered that it can have a function.

Furthermore, we also show that the anti-aging effect of concentrated birch sap is not simply linearly related to its concentration, but tends to first increase and then decrease with increasing concentration. That is, when the concentration of birch sap reaches a certain level, the concentrated birch sap suppresses cell proliferation and viability to some extent, and suppresses the expression of senescence-related genes inside and outside cells. Therefore, it is important to control the concentration of birch sap. In the present invention, the concentration of birch sap is controlled to about 1.05 to 8 times, preferably about 1.1 to 4 times, more preferably about 1.2 to 2 times.

In another aspect, the present invention relates to the use of concentrated birch sap in anti-aging cosmetic compositions.

In yet another aspect, the present invention relates to an anti-aging cosmetic composition comprising (A) the concentrated birch sap described above.

In a preferred embodiment, the anti-aging cosmetic composition is free of chelating agents such as EDTA salts, sodium polyphosphate, sodium metaphosphate and gluconic acid.

The anti-aging cosmetic composition does not contain added water, but does not exclude the water inherent in each of the composition's components.

The content of concentrated birch sap is about 18-98% by weight, preferably 20-95% by weight, more preferably 22-90% by weight, most preferably 30-90% by weight, based on the total weight of the anti-aging cosmetic composition. is.

Component (A) In addition to the concentrated birch sap described above, the anti-aging cosmetic composition optionally further comprises components commonly used in skin care cosmetic compositions, including (B) a vehicle, an active ingredient, and an excipient. may contain. Component (B) is known in the art, and its type and amount can be selected by those skilled in the art according to need. For example, the content of component (B) is about 2-82% by weight based on the total weight of the anti-aging cosmetic composition.

Vehicles include, for example, diluents, dispersants, or carriers. Examples include, but are not limited to, ethanol, dipropylene glycol, and butanediol. The content of vehicles in cosmetic compositions is known in the art, eg typically 0.5-20% based on the total weight of component (B).

Active ingredients include, for example, emollients, moisturizers, anti-aging active ingredients, and the like.

Examples of emollients include, but are not limited to, olive oil, macadamia nut oil, sweet almond oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, meadowfoam seed oil, safflower seed oil, Rosa canina fruit oil, Argania spinosa kernel oil, Simmondsia chinensis seed oil, sunflower seed oil, Mauritia flexuosa fruit oil, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated Polyisobutylene, isocetane, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosine, isononyl isononanoate, polydecene hydrogenation, triethylhexanoin, cetyl ethyl hexanoate, bis-diethoxydiglycol cyclohexane 1,4-dicarboxylate, capryl acid/capric acid triglyceride, oleyl erucate, octyldodecanol myristate, octyldodecanol, polydimethylsiloxane, octylpolymethylsiloxane, cetyldimethicone, cyclopentadimethylsiloxane, and the like. Examples of solid emollients include, but are not limited to, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, squalyl alcohol, lauric acid, myristic acid, palmitic acid, stearic acid, beeswax, candelilla wax, carnauba wax, Lanolin, ozokerite, jojoba seed wax, paraffin wax, microcrystalline wax, hydrogenated rice bran wax, hydrogenated cocoglyceryl, glyceryl behenate/eicosanedioate, myristyl myristate, bis-diglyceryl polyacyl adipate-2, butyrospermum perky (butyrospermum parkii) (shea butter), and astrocaryum murumuru seed oil. The content of emollients in cosmetic compositions is known in the art, eg typically 1-50% based on the total weight of component (B).

Examples of humectants include, but are not limited to, glycerol, diglycerol, butanediol, propylene glycol, 1,3-propanediol, dipropylene glycol, 1,2-pentanediol, polyethylene glycol-8, polyethylene glycol-32. , methylgluceth-10, methylgluceth-20, PEG/PPG-17/6 copolymer, glycereth-7, glycereth-26, glyceryl glucoside, PPG-10 methylglucose ether, PPG-20 methylglucose ether, PEG/PPG/polybutylene glycol - 8/5/3 Glycerol, Sucrose, Trehalose, Rhamnose, Mannose, Raffinose, Betaine, Erythritol, Xylitol, Urea, Glycereth-5 Lactate, Sodium Hyaluronate, Sodium Hydrolyzed Hyaluronate, Sodium Acetylated Hyaluronate, Sodium Polyglutamate , hydrolyzed sclerotium gum, pullulan, tremellam, and tamarindus indica seed polysaccharides. The content of humectants in cosmetic compositions is known in the art, eg typically 1-30% based on the total weight of component (B).

Examples of anti-aging active ingredients include, but are not limited to, tocopherol (vitamin E), retinol, retinyl palmitate, hydrolyzed collagen, hydrolyzed elastin, allantoin, yeast extract, oryzanol, tetrahydrocurcumin, ellagic acid, ubiquinone, milk. Pure protein, acetyl hexapeptide-8, palmitoyl pentapeptide-4, salicylophytosphingosine, birch sap concentrate, silymarin, sericin, sodium tocopheryl phosphate, ribonucleic acid (RNA), dipeptide diaminobutyroylbenzylamide diacetate , palmitoyl tripeptide-5, oligopeptide-1, hexapeptide-9, palmitoyl oligopeptide, palmitoyl tetrapeptide-7, grape (Vitis vinifera) seed extract, protecarpus (Pterocarpus marsupium) bark extract, tea tree (Camellia sinensis) ) polyphenols, wine extract, apple seed extract, Fagus sylvatica bud extract, African baobab (Adansonia digitata) extract, Artemia extract, Iris florentina root extract, hesperidin, ginsenosides, Salvia miltiorrhiza extract, nicotinamide, ursolic acid, sodium hyaluronate, acetylated sodium hyaluronate, hydrolyzed sodium hyaluronate, lycopene, Coffea arabica extract, dipeptide-2, lactic acid, Superoxide dismutase (SOD), evening primrose (Oenothera biennis) oil, ceramide, dipalmitoyl hydroxyproline, hydroxystearic acid, salicylic acid, ergothioneine, lysolecithin, carnosine, decarboxycarnosine HCL, lipoic acid, adenosine, glycogen, resveratrol, ferula One or more of acid, bifidobacterium culture lysis buffer and lactococcus culture lysis buffer. The content of anti-aging active ingredients in cosmetic compositions is known in the art, eg typically 0.01-10% based on the total weight of component (B).

Excipients include, for example, emulsifiers, thickeners, preservatives, flavoring agents, and the like.

Examples of emulsifiers include, but are not limited to, cetearyl oleate, sorbitan oleate, polysorbate-60, polysorbate-80, methylglucose sesquistearate, PEG-20 methylglucose sesquistearate, PEG-40 hydrogenated castor oil, PPG -26-buteth-26, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, steareth-2, steareth-21, PPG-13-decyltetradeceth-24, cetearyl glucoside, PEG- stearate 100, glyceryl stearate, glyceryl stearate SE, cocoglucoside, ceteareth-25, PEG-40 stearate, polyglyceryl-3 methylglucose distearate, glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, Polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose stearate, and polystearic acid one or more of sucrose. The content of emulsifiers in cosmetic compositions is known in the art, eg typically 0.5-10% based on the total weight of component (B).

Examples of thickening agents include, but are not limited to, carbomer, acrylates and derivatives thereof, xanthan gum, gum arabic, polyethylene glycol-14M, polyethylene glycol-90M, succinyl polysaccharides, hydroxyethylcellulose, hydroxypropylcellulose, and hydroxypropylmethylcellulose. and one or more of the macromolecular polymers of The content of thickeners in cosmetic compositions is known in the art, eg typically 0.1-10% based on the total weight of component (B).

Examples of preservatives include, but are not limited to, methyl hydroxybenzoate, propyl hydroxybenzoate, phenoxyethanol, benzyl alcohol, phenyl ethanol, bis(hydroxymethyl) imidazolidinyl urea, potassium sorbate, sodium benzoate, chlorphenesin ( chlorophenesin), sodium dehydroacetate, caprylhydroxamic acid, 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, caprylyl glycol, glyceryl caprylate, glyceryl undecylenate, sorbitan caprylate, ethylhexylglycerol, and One or more of peony root extracts may be included. The content of preservatives in cosmetic compositions is known in the art, eg typically 0.01-2% based on the total weight of component (B).

Anti-aging cosmetic compositions according to the present invention can be prepared by any suitable method known in the art. For example, the composition can be prepared using equipment commonly used in the cosmetic field, such as dissolving tanks, emulsifying pots, dispersers, transfer pumps, and the like. During preparation, the water-soluble substance is charged into the aqueous phase dissolution kettle, the oil-soluble substance is charged into the oil phase dissolution kettle, and the two kettles are each heated to about 80°C; Can be pre-dispersed using Once dissolution is complete, the oil and water phases are transferred to an emulsifying pot and homogenized and emulsified for about 5-15 minutes. Once emulsification is complete, the bulk is cooled to room temperature, optionally adding fragrances, preservatives, etc. and adjusting the pH of the product as necessary.

The above preparation method can be changed or adjusted according to the requirements of the product form. Anti-aging cosmetic compositions can be prepared into various product forms such as ointments, creams, milks, lotions, essences and the like.

Examples The invention is explained in further detail below with reference to examples. However, these examples and comparative examples are used only to illustrate the invention and should not be construed as limiting the scope of the appended claims of the invention in any way.

Example 1: Effect of undiluted birch sap and concentrated birch sap on the expression of senescence-related genes In this example, the effects of undiluted birch sap and concentrated birch sap with varying degrees of concentration were tested and compared on the expression of senescence-related genes. .

1. Birch Sap Concentration Daxinganling Chaoyue Wild Berry Development Co.; , Ltd. Fresh birch sap (Betula Alba sap) stock solution, purchased from They were concentrated to 1.2-fold, 1.5-fold, 2-fold, 4-fold and 8-fold, respectively.

2. Testing Experimental equipment: Fluorescent Quantitative PCR (Roche), Clean Bench (Su Jing), _CO2 Incubator (Binder), Microplate Reader (BIO-TEK), Micro Oscillator.
Experimental reagents and materials: human primary fibroblasts, 6-well culture plate, fibroblast culture medium, RNA extraction kit, reverse transcription kit, Trizol lysis buffer, etc.

The steps of fibroblast-based gene expression analysis were as follows:
(1) Seeding: Cells were seeded into 6-well culture plates at a density of 5E5 cells/well and incubated overnight at 37°C in an incubator containing 5% _CO2 ;
(2) Administration: when the cell seeding rate of the 6-well culture plate reached about 60%, each group of test substances was added thereto, providing 6 replicate wells for each group of test substances;
(3) Sample collection: After inoculation in an incubator containing 5% _CO2 for 24 hours at 37°C, the culture medium was discarded, 1 mL of Trizol was added to each well to lyse the cells, and then the samples were collected. ;
(4) PCR detection: RNA was extracted, reverse transcribed into cDNA and detected by fluorescent quantitative PCR;
(5) Analysis: The 2 ^-ΔΔCT method was used to calculate the results, and the T-test method was used for statistical analysis.

The test results are shown in the table below.

The above results show that compared to birch sap stock solution, birch sap concentrate with 1.1- to 4-fold enrichment significantly increased the expression level of fibroblast-based anti-aging related genes, especially enriched Gene expression levels of type I collagen, type III collagen, type IV collagen, type VII collagen, elastin and laminin are all significantly higher than those of birch sap undiluted solution when the degree is 1.2-2 fold. showed that

Example 2: Effect of undiluted birch sap and concentrated birch sap on the expression of anti-aging related proteins In this example, the effect of undiluted birch sap and concentrated birch sap with varying degrees of concentration on the expression of anti-aging related proteins was tested, compared.

1. Birch Sap Concentration Daxinganling Chaoyue Wild Berry Development Co.; , Ltd. Fresh birch sap (Betula Alba sap) stock solution, purchased from They were concentrated to 1.2-fold, 1.5-fold, 2-fold, 4-fold and 8-fold, respectively.

2. Testing Laboratory equipment: clean bench (Su Jing), immunowash microplate washer (BIO-RAD), microplate reader (BIO-TEK), CO ₂ incubator (Binder).
Experimental reagents and materials: human primary fibroblasts, 12-well culture plates, fibroblast culture medium, ELISA kits for various indicators, etc.

The test procedure was as follows:
(1) Inoculation: Cells were seeded into 12-well culture plates at a density of 2E5 cells/well, incubated at 37°C in an incubator containing 5% _CO2 , and the culture medium was changed every two days;
(2) Administration: When the cell seeding rate of the 12-well culture plate reached about 60%, each group of test substance was added thereto, providing 6 replicate wells for each group of test substance;
(3) Sample collection: After inoculation in an incubator containing 5% _CO2 for 48 hours at 37°C, the culture medium was discarded, 1 mL of Trizol was added to each well to lyse the cells, and then the samples were collected. ;
(4) Detection: Indicators were determined according to the determination method of the ELISA kit.
(5) Analysis: T-test method was used for statistical analysis.

The test results are shown in the table below.

The above results show that compared to birch sap stock solution, birch sap concentrate with 1.1- to 4-fold enrichment significantly increased the expression level of fibroblast-based anti-aging related proteins, especially enriched The protein expression levels of collagen type I, collagen type III, type IV, type VII, elastin and laminin are all significantly higher than the gene expression level of the birch sap undiluted solution when the degree is 1.2-2 fold. showed that

Example 3: Effect of Birch Sap Stock Solution and Concentrated Birch Sap on Tissue (Recombinant 3D Full Thickness Skin Model) Effects were tested and compared.

1. Birch Sap Concentration Daxinganling Chaoyue Wild Berry Development Co.; , Ltd. A fresh birch sap (Betula Alba sap) stock solution purchased from PT. Circulated until concentrated to the extent of .1, 1.2, 1.5, 2 and 4 times respectively.

2. Test method Laboratory equipment: clean bench (Su Jing), incubator (Binder), UVA and UVB irradiator, microplate reader (BIO-TEK), freezing microtome (Leica).
Experimental reagents and materials: 3D full-thickness skin model (homemade in the lab), ELISA kits for various indicators, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) kit etc.

The test procedure was as follows:
(1) Construction of 3D full-thickness skin model: A 3D full-thickness skin model was constructed using keratinocytes and fibroblasts;
(2) Modeling and dosing: When the model leaves the factory, i.e. day 0, SSUV irradiation treatment (UVA: 30 J/cm ² ; UVB: 50 mJ/cm ² ) is performed daily, and then a constant amount of sample is applied to the corresponding model control, model culture medium alone was applied, providing 6 replicate wells for each group of test substances, applications were performed once daily for a total of 4 days;
(3) Sample collection and detection: After modeling and dosing, culture medium was collected and MMP-1 (matrix metalloproteinase 1) was determined using an ELISA kit; 3 models were obtained from each group of test substances. , MTT kit was used to determine cell proliferation viability to obtain MTT values; determined the content;
(4) Analysis: T-test method was used for statistical analysis.

The test results are shown in the table below.

The MTT value results showed that birch sap concentrates with 1.1- to 4-fold concentration significantly increased the tissue vitality of the 3D full-thickness skin model compared to the birch sap undiluted solution. Similarly, the relative expression levels of type IV collagen at the junction between epidermis and dermis using birch sap concentrates with 1.1- to 4-fold enrichments were significantly higher than those of birch sap undiluted solution. was expensive. The MMP-1 results showed that birch sap concentrates with 1.1- to 4-fold enrichment were significantly superior to birch sap stock solutions. The above results also show that the tissue vitality of the recombinant 3D full-thickness skin model is significantly improved when the concentration of birch sap is 1.1- to 2-fold, with type IV We have shown that the relative expression levels of collagen can be enhanced and the expression of MMP-1 in the extracellular matrix can be decreased.

Example 4: Preparation of anti-aging face cream composition The formulation of the anti-aging face cream composition is shown in the table below:

An anti-aging face cream composition was prepared as follows:
1. Oil phase: Raw materials 4, 6, 7, 8, 9, 11, 12, 13, 15 and 21 were added to the oil phase pot, heated to 80°C, dissolved and mixed well;
2. Raw materials 2, 16 and 18 were mixed well at room temperature;
3. Raw materials 10, 14, 17, 20 were mixed well at room temperature;
4. Aqueous phase: Raw materials 1, 3 and 5 were heated to 80°C and the mixture from step 2 was added, dissolved and mixed well;
5. Emulsification: Add water phase and oil phase to emulsification tank, hold at 80°C, homogenize and emulsify at 3000 rpm for 5 minutes, add raw material 19 when emulsification is completed;
6. Upon cooling to 40° C. with stirring, the mixture from step 3 was added, stirred well and discharged to obtain an anti-aging face cream composition.

In this example, the effect of an anti-aging cream composition on a recombinant 3D full thickness skin model was tested.

The results show that the anti-aging cream composition very significantly increased the tissue vitality of the recombinant 3D full-thickness skin model, promoted the relative expression levels of type IV collagen at the junction between the epidermis and dermis, and the cell We have shown that the expression of MMP-1 in the outer matrix can be decreased.

Example 5: Preparation of anti-aging eye cream composition The formulation of the anti-aging eye cream composition is shown in the table below:

An anti-aging eye cream composition was prepared as follows:
1. Oil phase: Raw materials 3, 5, 6, 7, 9 and 11 were added to the oil phase pot, heated to 80°C, dissolved and mixed well;
2. Ingredients 2 and 16 were mixed well at room temperature;
3. Raw materials 10, 14 and 17 were mixed well at room temperature;
4. Aqueous phase: Raw materials 1, 4, 8, 13, 18, 19 and 20 were heated to 80°C and the mixture from step 2 was added, dissolved and mixed well;
5. Emulsification: Add water phase and oil phase to emulsification tank, hold at 80°C, homogenize and emulsify at 3000 rpm for 5 minutes, add ingredients 12 and 15 when emulsification is completed;
6. Upon cooling to 40° C. with stirring, the mixture from step 3 was added, stirred well and discharged to obtain an anti-aging eye cream composition.

Using a half-face control test method, 20 subjects were tested before using the product and after 4 weeks of using the product as follows:
1) Primos was used to photograph the subject's left and right outer corners of the eye, and software was used to calculate wrinkle parameters, including wrinkle volume, wrinkle area, wrinkle depth, etc.; and 2) using Corneometer. and tested the skin moisture content of the left and right corners of the eyes;

The results showed that 18 out of 20 subjects had significantly increased skin hydration in the outer corner of the eye, and 15 of these had significantly shallower and lighter eye wrinkles, decreased wrinkle area, and decreased wrinkle volume. Ta.

Example 6: Preparation of anti-aging emulsion composition The formulation of the anti-aging emulsion composition was as follows:

An anti-aging emulsion composition was prepared as follows:
1. Oil phase: Raw materials 4, 5, 6, 7, 12 and 13 were added to the oil phase pot, heated to 80°C, dissolved and mixed well;
2. Raw materials 2, 8 and 11 were mixed well at room temperature;
3. Ingredients 9, 10 and 16 were mixed well at room temperature;
4. Aqueous Phase: Raw materials 1, 3 and 14 were heated to 80° C. and the mixture from step 2 was added, dissolved and mixed well;
5. Emulsification: Add water phase and oil phase to emulsification tank, hold at 80°C, homogenize and emulsify at 3000 rpm for 5 minutes, add raw material 15 when emulsification is completed;
6. Upon cooling to 40° C. with stirring, the mixture from step 3 was added, stirred well and discharged to obtain an anti-aging emulsion composition.

Twenty subjects were treated with the emulsion composition for four weeks and subjected to subjective evaluation. Among them, 18 reported a significant increase in skin hydration and smooth, delicate and elastic skin; 16 reported significant improvement in nasolabial folds and eye wrinkles.

Example 7: Anti-Aging Essence Composition The formulation of the anti-aging essence composition is shown in the table below:

An anti-aging essence composition was prepared as follows:
1. Raw materials 1, 2, 3, 4, 6, 12 and 13 were added to the emulsification tank and held at 80°C;
2. Raw materials 5, 10 and 11 were mixed well at room temperature;
3. Ingredients 7, 8 and 9 were mixed well at room temperature;
4. Raw material 14 was added and the pH was adjusted;
5. Upon cooling to 40° C. with stirring, raw materials 15 and 16 and the mixture from step 3 were added, stirred well and discharged to obtain an anti-aging essence composition.

Using a half-face control test method, 20 subjects were tested before using the product and after 8 weeks of using the product as follows:
1) Primos was used to photograph the subject's left and right outer corners of the eye, and software was used to calculate wrinkle parameters, including wrinkle amount, wrinkle area, wrinkle depth, etc.;
2) The corneometer was used to test the skin hydration of the left and right outer corners of the eyes;
3) The transepidermal water loss (TEWL value) of the left and right cheeks was tested using a Tewameter.

The results showed that 18 out of 20 subjects had significantly increased skin hydration in the outer corner of the eye, and 15 of these had significantly shallower and lighter eye wrinkles, decreased wrinkle area, and decreased wrinkle volume. Ta. Eighteen of the 20 subjects had significantly increased skin hydration and significantly decreased TEWL values, of which 16 had significantly shallower and lighter nasolabial folds and eye wrinkles, decreased wrinkle area, and It showed a reduction in the amount of wrinkles.

The technical solutions in the above examples are preferred embodiments of the present invention. Various modifications and variations may be made without departing from the spirit and principles of the invention, and these modifications and variations should also be considered within the scope of the invention.

Claims (3)

Use of concentrated birch sap in an anti-aging cosmetic composition, said concentrated birch sap having a concentration of 1.1 to 4 times,
Use , wherein said anti-aging cosmetic composition further comprises no added water . An anti-aging cosmetic composition comprising concentrated birch sap, wherein said concentrated birch sap has a concentration of 1.1 to 4 times,
An anti-aging cosmetic composition, wherein said anti-aging cosmetic composition further comprises no added water . 3. The anti-aging cosmetic composition according to claim 2 , comprising 18-98% by weight of concentrated birch sap, based on the total weight of the anti-aging cosmetic composition.