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JP7330095B2 - Method for producing softened food - Google Patents
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JP7330095B2 - Method for producing softened food - Google Patents

Method for producing softened food Download PDF

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JP7330095B2
JP7330095B2 JP2019236311A JP2019236311A JP7330095B2 JP 7330095 B2 JP7330095 B2 JP 7330095B2 JP 2019236311 A JP2019236311 A JP 2019236311A JP 2019236311 A JP2019236311 A JP 2019236311A JP 7330095 B2 JP7330095 B2 JP 7330095B2
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enzyme
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晴雄 冨田
理奈 中嶋
章 宮藤
圭一 荒木
瞳 三木
秀和 羽山
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Osaka Gas Co Ltd
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Description

本発明は、肉類からなる軟化食品を製造する方法に関する。 TECHNICAL FIELD The present invention relates to a method for producing a softened meat product.

近年の高齢化に伴い、嚥下障害を有する者や咀嚼力が低下した者が増加していることから、歯茎や舌で容易に潰すことができる軟化食品(所謂、やわらか食)の開発が求められている。従来の軟化食品は、食材を細かく刻む、ミキサー等ですり潰すといった手法を用いて製造されていた。しかしながら、これらの手法で製造した軟化食品は、食材が原形をとどめておらず、見た目や食味が元の食材と比較して悪化していることで、軟化食品を食する者の食欲の減退を招いていた。 With the aging population in recent years, the number of people with dysphagia and decreased chewing power is increasing, so there is a demand for the development of soft food that can be easily crushed with the gums or tongue (so-called soft food). ing. Conventional softened foods have been produced using methods such as finely chopping ingredients or grinding them with a mixer or the like. However, the softened food produced by these methods does not retain its original shape, and its appearance and taste are worse than those of the original food. was inviting

そこで、近年、食品業界では、食材の形状をできる限り維持したまま食材を軟化させる方法の一つとして、酵素によりタンパク質などの基質を分解して食材を軟化させる方法に注目が集まっている。 Therefore, in recent years, in the food industry, as one of the methods of softening the food while maintaining the shape of the food as much as possible, a method of softening the food by decomposing substrates such as proteins with enzymes is attracting attention.

例えば、特許文献1には、魚介類を原材料とする加工食品を製造する方法が開示されており、この特許文献1に開示された方法によれば、魚介類中の酵素基質を所定の分解酵素の作用によって分解させる工程を行うことで、形状を保持した柔軟な食感を有する加工食品を製造することができる。 For example, Patent Document 1 discloses a method for producing processed foods using fish and shellfish as raw materials. By performing the step of decomposing by the action of , it is possible to produce a processed food that retains its shape and has a soft texture.

特許第4986188号公報Japanese Patent No. 4986188

ところで、肉類は、酵素処理によって基質の分解が進み過ぎると、未処理の肉類と異なった味や食感を伴う食味となり、軟化食品を食する者は、未処理の肉類の食味との違いに違和感を抱くことになる。また、単純に酵素を浸透させただけの肉類を酵素の活性温度まで加熱して軟化食品を製造した場合、酵素処理後の肉類の見た目や形状が悪化し、軟化食品を食する者は、未処理の肉類との見た目や形状の違いにも違和感を抱くことになる。そして、このような違和感が原因で軟化食品を食する者の食欲が減退するという問題がある。 By the way, if the substrate of meat is degraded too much by enzymatic treatment, the taste and texture of meat will differ from those of untreated meat. You will feel uncomfortable. In addition, when meat that has simply been impregnated with enzymes is heated to the enzyme activation temperature to produce softened food, the appearance and shape of the meat after the enzyme treatment deteriorates, and those who eat the softened food may not The difference in appearance and shape from processed meat is also uncomfortable. In addition, there is a problem that the appetite of the person who eats the softened food is reduced due to such discomfort.

それゆえ、酵素処理によって基質を分解し、肉類を軟化させて製造する軟化食品は、食味の悪化が抑えられ、軟化食品を食するものが違和感を抱き難い形状を有しており、適度な硬さに軟化したものであることが望まれるが、そのような軟化食品の製造方法は依然として確立されていないのが現状である。 Therefore, the softened food produced by decomposing the substrate by enzymatic treatment and softening the meat can suppress the deterioration of the taste, has a shape that makes it difficult for those who eat the softened food to feel uncomfortable, and has a moderate hardness. Although it is desired that the food is softened to a certain extent, the current situation is that a method for producing such a softened food has not yet been established.

本発明は以上の実情に鑑みなされたものであり、肉類を原材料とする軟化食品であって、十分な柔らかさを備えつつ、任意の形状を有し、食味の悪化が抑えられた軟化食品を製造できる方法の提供を、その目的とする。 The present invention has been made in view of the above circumstances, and provides a softened food made of meat as a raw material, which has sufficient softness, has an arbitrary shape, and has suppressed deterioration in taste. The purpose is to provide a method that can be manufactured.

上記目的を達成するための本発明に係る軟化食品製造方法の特徴構成は、肉類からなる軟化食品を製造する方法であって、
前記肉類を所定温度まで加熱する前処理工程と、
加熱した前記肉類を凍結乾燥する凍結乾燥工程と、
凍結乾燥した前記肉類を粉砕する粉砕工程と、
粉砕した前記肉類からなる粉粒体の少なくとも一部に、前記肉類中の所定の基質を分解する酵素を含む処理液を浸透させて、前記処理液が浸透した部位をペースト状にする浸透工程と、
前記処理液を浸透させた粉粒体を、前記酵素の活性温度まで加熱する加熱工程とを実施する点にある。
The characteristic configuration of the method for producing a softened food product according to the present invention for achieving the above object is a method for producing a softened food product made of meat,
A pretreatment step of heating the meat to a predetermined temperature;
a freeze-drying step of freeze-drying the heated meat;
A pulverizing step of pulverizing the freeze-dried meat;
A permeation step of infiltrating at least a part of the granules made of the pulverized meat with a treatment liquid containing an enzyme that decomposes a predetermined substrate in the meat , and turning the portion infiltrated with the treatment liquid into a paste. and,
and a heating step of heating the granules infiltrated with the treatment liquid to the activation temperature of the enzyme.

上記特徴構成によれば、前処理工程において肉類を所定温度まで加熱することによって、肉類中の所定の基質が適度に分解する。このとき、基質がタンパク質であれば、前処理工程においてタンパク質が適度に変性するとともに、タンパク質複合体が適度に複数のサブユニットに分解する。その後、基質が適度に分解した肉類(基質がタンパク質であれば、適度に変性、分解した肉類)を凍結乾燥することにより、基質が適度に分解等した状態を維持しつつ、肉類を乾燥させる。ついで、凍結乾燥によって乾燥させた肉類を粉砕し、その後、粉砕した肉類からなる粉粒体の少なくとも任意の一部に酵素を含む処理液を浸透させ、この処理液を浸透させた粉粒体を酵素の活性温度まで加熱する。これにより、酵素による分解作用によって肉類中の基質が分解されて肉類が軟化するとともに、粉砕した肉類のうち処理液が浸透していた部分の肉類同士が結着する。つまり、浸透工程において、粉砕した肉類からなる粉粒体に処理液を浸透させる際に、処理液を浸透させる部分の大きさや形を調整することで、加熱工程後に得られる軟化食品が十分な柔らかさを備えつつ、任意の形状を有したものとなる。 According to the above characteristic configuration, by heating the meat to a predetermined temperature in the pretreatment step, the predetermined substrate in the meat is appropriately decomposed. At this time, if the substrate is protein, the protein is moderately denatured and the protein complex is moderately decomposed into a plurality of subunits in the pretreatment step. After that, the meat whose substrate is moderately decomposed (if the substrate is protein, the meat which is moderately denatured and decomposed) is freeze-dried to dry the meat while maintaining the state of moderately decomposed substrate. Next, the meat dried by freeze-drying is pulverized, and then at least an arbitrary part of the granules made of the pulverized meat is permeated with a treatment liquid containing an enzyme, and the granules permeated with the treatment liquid are Heat up to enzyme activity temperature. As a result, the substrate in the meat is decomposed by the decomposing action of the enzyme, the meat is softened, and the portions of the pulverized meat that have been permeated with the treatment liquid bind to each other. In other words, in the impregnation step, when the treatment liquid is impregnated into the granular material made of pulverized meat, by adjusting the size and shape of the portion to be impregnated with the treatment liquid, the softened food obtained after the heating process is sufficiently soft. It has an arbitrary shape while being equipped with a sturdiness.

また、本発明に係る軟化食品製造方法では、上記のように、前処理工程後に凍結乾燥工程を実施することで、肉類中の基質が分解された状態(基質がタンパク質であれば、タンパク質複合体が適度に複数のサブユニットに分解し、且つ、タンパク質が適度に変性した状態)が維持される。ここで、基質が適度に分解した状態であると、酵素による基質の分解が進み易くなる。基質がタンパク質である場合、タンパク質複合体がサブユニットに分解した状態であると、酵素によるタンパク質の分解が進み易くなり、また、タンパク質が適度に変性した状態であると、変性したタンパク質に酵素が作用しないため、変性していないタンパク質が酵素によって分解されることになる。したがって、基質が適度に分解した状態が維持された肉類を粉砕し、この粉砕した肉類に対して酵素を作用させることで、基質が適度に分解されるため、加熱工程後に得られる軟化食品は、食味の悪化が抑えられつつ、十分な柔らかさを備えたものとなる。特に、基質がタンパク質である場合には、タンパク質複合体がサブユニットに分解した状態、且つ、タンパク質が適度に変性した状態が維持された肉類を粉砕し、この粉砕した肉類に対して酵素を作用させることになる。そのため、肉類中に残存するタンパク質複合体の量が減少するとともに、肉類中のタンパク質が適度に分解される。したがって、得られる軟化食品は、単に肉類を酵素で処理したものと比較して、食味の悪化が抑えられ、十分な柔らかさを備えたものとなる。 In addition, in the softened food production method according to the present invention, as described above, the freeze-drying step is performed after the pretreatment step, so that the substrate in the meat is decomposed (if the substrate is protein, the protein complex is moderately degraded into a plurality of subunits, and the protein is moderately denatured) is maintained. Here, when the substrate is in a moderately decomposed state, the decomposition of the substrate by the enzyme is facilitated. When the substrate is a protein, when the protein complex is decomposed into subunits, the protein is easily decomposed by the enzyme. Since it does not act, the undenatured protein will be degraded by the enzyme. Therefore, by pulverizing the meat in which the substrate is maintained in a moderately decomposed state and allowing the enzyme to act on the pulverized meat, the substrate is moderately decomposed, so the softened food obtained after the heating process is It has sufficient softness while suppressing deterioration of taste. In particular, when the substrate is a protein, the meat in which the protein complex is decomposed into subunits and the protein is maintained in a moderately denatured state is pulverized, and the enzyme is applied to the pulverized meat. will let you Therefore, the amount of protein complexes remaining in the meat is reduced, and the protein in the meat is moderately decomposed. Therefore, the resulting softened food is less spoiled in taste and has sufficient softness as compared to meat simply treated with enzymes.

このように、本発明に係る軟化食品製造方法によれば、肉類を原材料とする軟化食品であって、十分な柔らかさを備えつつ、任意の形状を有し、食味の悪化が抑えられた軟化食品を製造することができる。 As described above, according to the method for producing a softened food product according to the present invention, a softened food product using meat as a raw material has sufficient softness, has an arbitrary shape, and is softened so that deterioration in taste is suppressed. Food can be manufactured.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記浸透工程において、前記粉粒体における任意の位置に前記処理液を微滴化して吹き付けて、前記粉粒体における任意の位置に前記処理液を浸透させる点にある。 Further, a further characteristic configuration of the softened food manufacturing method according to the present invention is that, in the permeation step, the treatment liquid is formed into fine droplets and sprayed onto an arbitrary position in the granular material, The point is that the treatment liquid is permeated into the substrate.

上記特徴構成を備えた軟化食品製造方法によれば、所謂インクジェット方式によって、粉砕した肉類からなる粉粒体の任意の位置に処理液を微滴化して吹き付けて、任意の位置に処理液を浸透させることができる。そして、この軟化食品製造方法では、任意の位置に処理液が浸透した状態の肉類に対して加熱工程を実施することで、処理液が浸透した部分が結着した状態の十分に柔らかい軟化食品を得ることができる。このように、上記特徴構成を備えた軟化食品製造方法では、粉砕した肉類の任意の位置に処理液を浸透させる際に、所謂インクジェット方式を用いていることで、単に処理液を滴下させるような場合と比較して、処理液を浸透させる位置を細かく調整することができる。したがって、バリエーション豊かな形状の軟化食品を容易に製造することができる。 According to the softened food manufacturing method having the above-described characteristic configuration, the treatment liquid is formed into fine droplets and sprayed onto an arbitrary position of the granules made of pulverized meat by a so-called inkjet method, and the treatment liquid permeates the arbitrary position. can be made In this method for producing a softened food product, a heating step is performed on the meat in which the treatment liquid has permeated at an arbitrary position, thereby producing a sufficiently soft softened food in a state where the portion permeated with the treatment liquid is bound. Obtainable. As described above, in the softened food manufacturing method having the above-described characteristic configuration, when the treatment liquid is permeated into an arbitrary position of the pulverized meat, the so-called inkjet method is used, so that the treatment liquid is simply dripped. Compared with the case, the position to be permeated with the treatment liquid can be finely adjusted. Therefore, it is possible to easily produce softened foods with a wide variety of shapes.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記粉砕工程において、前記凍結乾燥した肉類を、平均粒子径が1μm以上1mm以下となるように粉砕する点にある。 A further characteristic configuration of the softened food production method according to the present invention is that the freeze-dried meat is pulverized in the pulverization step so that the average particle size is 1 μm or more and 1 mm or less.

粉砕した肉類の平均粒子径が小さすぎたり、大きすぎたりする場合、粉砕した肉類の間への処理液が浸透しすぎたり、逆に浸透しなかったりする虞がある。そのため、粉砕工程においては、凍結乾燥した肉類を平均粒子径が1μm以上1mm以下となるように粉砕することが好ましい。尚、本願発明者は、平均粒子径が1μm以上1mm以下であるように粉砕した肉類に対して浸透工程及び加熱工程を実施することで、十分な柔らかさを備えつつ、所望の形状を有し、食味の悪化が抑えられた軟化食品を製造できることを実験的に確認している。 If the average particle size of the pulverized meat is too small or too large, there is a risk that the treatment liquid may penetrate too much or not permeate the pulverized meat. Therefore, in the pulverization step, it is preferable to pulverize the freeze-dried meat so that the average particle size is 1 μm or more and 1 mm or less. In addition, the inventor of the present application has a desired shape while having sufficient softness by performing a permeation step and a heating step on meat that has been pulverized so that the average particle size is 1 μm or more and 1 mm or less. , confirmed experimentally that it is possible to produce a softened food in which the deterioration of taste is suppressed.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記前処理工程において、前記肉類を70℃以上に加熱する点にある。 A further characteristic configuration of the softened food manufacturing method according to the present invention is that the meat is heated to 70° C. or higher in the pretreatment step.

前処理工程における肉類の加熱が不十分であると、基質が適度に分解した状態とならない場合がある。このような場合、十分な柔らかさを備えた軟化食品を得るために、酵素の濃度を高くする、或いは、加熱工程の時間を長くするといった対応が必要となる。特に、酵素の濃度を高くしなければならない場合、酵素自身の味が軟化食品の味に強く現れ、食味が悪化し易くなる。また、基質がタンパク質である場合には、タンパク質が変性しないか或いは適度に変性した状態とならない場合や、肉類中のタンパク質複合体の分解が不十分になる場合がある。このような場合、酵素による分解作用によってタンパク質が必要以上に分解された状態となったり、タンパク質複合体の残存量が多くなったりすることで、食味が悪化したり、十分な柔らかさを有したものにならなかったりする虞がある。 If the meat is insufficiently heated in the pretreatment step, the substrate may not be appropriately decomposed. In such a case, in order to obtain softened food with sufficient softness, measures such as increasing the concentration of the enzyme or extending the time of the heating process are required. In particular, when the concentration of the enzyme must be increased, the taste of the enzyme itself strongly appears in the taste of the softened food, and the taste tends to deteriorate. Moreover, when the substrate is protein, the protein may not be denatured or may not be appropriately denatured, or the protein complex in meat may not be sufficiently decomposed. In such a case, the protein is degraded more than necessary due to the decomposition action of the enzyme, or the remaining amount of the protein complex increases, which deteriorates the taste or causes the product to have insufficient softness. There is a fear that it will not become a thing.

しかしながら、上記特徴構成によれば、前処理工程において、肉類を70℃以上に加熱することで、基質が適度に分解した状態となるため、酵素の濃度を高くする、或いは、加熱工程の時間を長くするといった対応が不要となる。また、基質がタンパク質である場合には、酵素による分解作用を受けるタンパク質(変性していないタンパク質)の量を適度な量にすることができるため、酵素によって肉類中のタンパク質を適度に分解することができる。また、タンパク質複合体を適度に分解することができる。 However, according to the above characteristic configuration, in the pretreatment step, by heating the meat to 70 ° C. or higher, the substrate is in a state of being appropriately decomposed. There is no need to deal with lengthening the time. In addition, when the substrate is protein, the amount of protein (undenatured protein) subjected to enzymatic decomposition can be reduced to an appropriate amount. can be done. In addition, protein complexes can be moderately decomposed.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記酵素を含む処理液は、前記酵素の濃度が0.001質量%以上1質量%以下である点にある。 A further characteristic configuration of the softened food manufacturing method according to the present invention is that the concentration of the enzyme in the treatment liquid containing the enzyme is 0.001% by mass or more and 1% by mass or less.

肉類に浸透させる処理液中の酵素濃度が高すぎると、酵素自身の味が軟化食品の味に強く現れすぎたり、基質が分解され過ぎたりすることで食味が悪化する。一方、処理液中の酵素濃度が低すぎると、分解作用が弱くなり過ぎて処理に要する時間が増大する。上記特徴構成を備えた軟化食品製造方法によれば、処理液中の酵素の濃度が0.001質量%以上1質量%以下であることで、酵素自身の味が軟化食品の味に強く現れることもなく、肉類が分解され過ぎるということもない。そのため、得られる軟化食品は、十分な柔らかさを備えつつ、所望の形状を有し、食味の悪化が抑えられたものとなる。また、製造に要する時間が極端に増大するといった問題も生じない。 If the concentration of the enzyme in the treatment solution to be permeated into the meat is too high, the taste of the enzyme itself appears too strongly in the taste of the softened food, or the substrate is decomposed too much, resulting in poor taste. On the other hand, if the enzyme concentration in the treatment solution is too low, the decomposition action will be too weak and the time required for the treatment will increase. According to the method for producing a softened food product having the above characteristic configuration, the concentration of the enzyme in the treatment liquid is 0.001% by mass or more and 1% by mass or less, so that the taste of the enzyme itself appears strongly in the taste of the softened food product. No meat is overly decomposed. Therefore, the obtained softened food has a desired shape while being sufficiently soft, and the deterioration of the taste is suppressed. In addition, there is no problem that the time required for manufacturing is extremely increased.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記酵素は、プロテアーゼである点にある。 A further characteristic configuration of the softened food manufacturing method according to the present invention is that the enzyme is a protease.

上記特徴構成によれば、肉類中のタンパク質を分解して当該肉類を柔らかくでき、得られる軟化食品は、十分な柔らかさを備えたものとなる。 According to the above characteristic configuration, the protein in the meat can be decomposed to soften the meat, and the obtained softened food has sufficient softness.

また、本発明に係る軟化食品製造方法の更なる特徴構成は、前記前処理工程において、前記肉類を蒸すことで所定温度まで加熱する点にある。 A further characteristic configuration of the softened food manufacturing method according to the present invention is that the meat is heated to a predetermined temperature by steaming in the pretreatment step.

上記特徴構成によれば、前処理工程において肉類を蒸すようにしていることで、調理中のドリップの量が少なくなり、得られる軟化食品の食味が悪化し難くなる。 According to the above characteristic configuration, by steaming the meat in the pretreatment step, the amount of dripping during cooking is reduced, and the resulting softened food is less likely to deteriorate in taste.

以下、本発明の実施形態に係る軟化食品製造方法について説明する。尚、以下に好適な実施例を記すが、これら実施例はそれぞれ、本発明をより具体的に例示するために記載されたものであって、本発明の趣旨を逸脱しない範囲において種々変更が可能であり、本発明は、以下の記載に限定されるものではない。 Hereinafter, a softened food manufacturing method according to an embodiment of the present invention will be described. Although preferred examples are described below, each of these examples is described in order to illustrate the present invention more specifically, and various changes can be made without departing from the scope of the present invention. and the present invention is not limited to the following description.

[軟化食品製造方法]
実施形態に係る軟化食品製造方法は、肉類からなる軟化食品を製造する方法であって、肉類を所定温度まで加熱する前処理工程と、加熱した肉類を凍結乾燥する凍結乾燥工程と、凍結乾燥した肉類を粉砕する粉砕工程と、粉砕した肉類からなる粉粒体の少なくとも一部に、当該肉類中の所定の基質を分解する酵素を含む処理液を浸透させる浸透工程と、処理液を浸透させた粉粒体を酵素の活性温度まで加熱する加熱工程とを実施する。尚、以下においては、基質がタンパク質である場合を例にとって説明するが、基質はタンパク質に限られるものではない。
[Softening food manufacturing method]
A method for producing a softened food according to an embodiment is a method for producing a softened food made of meat, and includes a pretreatment step of heating the meat to a predetermined temperature, a freeze-drying step of freeze-drying the heated meat, and a freeze-dried A pulverizing step of pulverizing meat, a permeating step of infiltrating at least part of the granules made of the pulverized meat with a treatment liquid containing an enzyme that decomposes a predetermined substrate in the meat, and permeating with the treatment liquid. and a heating step of heating the granules to the activity temperature of the enzyme. In the following description, the case where the substrate is protein will be described as an example, but the substrate is not limited to protein.

この軟化食品製造方法においては、前処理工程において肉類を所定温度(例えば70℃)まで加熱することによって、肉類中のタンパク質が適度に変性、分解する。その後、このタンパク質が適度に変性、分解した肉類を凍結乾燥することにより、タンパク質が変性、分解した状態を維持しつつ、肉類を乾燥させる。しかる後、凍結乾燥により乾燥させた肉類を粉砕し、粉砕した肉類からなる粉粒体における任意の部分に酵素を含む処理液を浸透させ、当該酵素を浸透させた粉粒体を酵素の活性温度まで加熱する。これにより、酵素による分解作用によって肉類中のタンパク質が分解されて肉類が軟化するとともに、粉砕した肉類のうち処理液が浸透していた任意の部分の肉類同士が結着する。したがって、この軟化食品製造方法によれば、得られる軟化食品は、十分な柔らかさを備えつつ、任意の形状を有したものとなる。 In this softened food production method, the meat is heated to a predetermined temperature (for example, 70° C.) in the pretreatment step, thereby moderately denaturing and decomposing the proteins in the meat. After that, the meat in which the protein is moderately denatured and decomposed is freeze-dried, thereby drying the meat while maintaining the denatured and decomposed state of the protein. Thereafter, the meat dried by freeze-drying is pulverized, an arbitrary part of the pulverized meat is impregnated with an enzyme-containing treatment liquid, and the enzyme-permeated powder is heated to the activity temperature of the enzyme. heat to As a result, the protein in the meat is decomposed by the decomposing action of the enzyme, the meat is softened, and arbitrary portions of the pulverized meat that have been permeated with the treatment liquid bind to each other. Therefore, according to this softened food manufacturing method, the softened food obtained has an arbitrary shape while being sufficiently soft.

前処理工程においては、具体的に、蒸す、焼く、煮る、炒めるといった加熱方法を用いて肉類を加熱し、肉類中のタンパク質を適度に変性、分解する。尚、肉類を蒸して加熱することで、調理中のドリップが少なくなり、軟化食品の食味が悪化し難くなる。したがって、前処理工程では、肉類を蒸すことで所定温度まで加熱することが好ましい。尚、所定温度としては、タンパク質を適度に変性、分解するという点で、70℃以上120℃以下とすることが好ましい。 In the pretreatment step, specifically, the meat is heated using a heating method such as steaming, grilling, boiling, or frying, and the proteins in the meat are appropriately denatured and decomposed. By steaming and heating the meat, dripping during cooking is reduced, and the taste of the softened food is less likely to deteriorate. Therefore, in the pretreatment step, it is preferable to heat the meat to a predetermined temperature by steaming. The predetermined temperature is preferably 70° C. or higher and 120° C. or lower from the viewpoint of appropriately denaturing and decomposing the protein.

ここで、この軟化食品製造方法では、前処理工程を実施することで、以下のような利点がある。即ち、前処理工程を実施することによって、肉類中のタンパク質複合体を予め適度に複数のサブユニットに分離した状態にできるため、軟化食品中にタンパク質複合体が残存し難くなり、また、後工程で酵素によるタンパク質の分解が進み易くなる。更に、前処理工程を実施することで、肉類中のタンパク質が予め適度に変性した状態となり、この変性したタンパク質には酵素が作用しないため、変性していないタンパク質が酵素によって分解されることになる。このように、この軟化食品製造方法によれば、前処理工程を実施することによって、肉類中に残存するタンパク質複合体の量が減少するとともに、タンパク質が適度に分解されるようになる。そのため、得られる軟化食品は、十分な柔らかさを備えつつ、食味の悪化が抑えられたものとなる。 Here, in this softened food manufacturing method, the following advantages are obtained by carrying out the pretreatment step. That is, by performing the pretreatment step, the protein complexes in the meat can be appropriately separated into a plurality of subunits in advance, so that the protein complexes are less likely to remain in the softened food, and the post-treatment step is performed. Enzymatic degradation of proteins is facilitated. Furthermore, by performing the pretreatment step, the proteins in the meat are in a state of being appropriately denatured in advance, and the enzymes do not act on the denatured proteins, so the undenatured proteins are decomposed by the enzymes. . Thus, according to this softened food production method, the pretreatment step reduces the amount of protein complexes remaining in the meat and moderately decomposes the protein. Therefore, the obtained softened food has sufficient softness while suppressing the deterioration of taste.

凍結乾燥工程においては、例えば、超低温冷凍機や液体窒素などを用いて前処理工程後の肉類を凍結した後、凍結後の肉類を既知の凍結乾燥装置を用いて乾燥させる。これにより、肉類は、タンパク質が適度に変性、分解した状態が維持されたまま乾燥される。尚、乾燥させる期間としては、例えば数時間~3日程度である。 In the freeze-drying step, for example, the meat after the pretreatment step is frozen using an ultra-low temperature refrigerator, liquid nitrogen, or the like, and then the frozen meat is dried using a known freeze-drying apparatus. As a result, the meat is dried while the protein is maintained in a moderately denatured and decomposed state. The drying period is, for example, several hours to three days.

粉砕工程においては、例えば、凍結乾燥後の肉類を乳鉢等で粉砕して粉粒体とする。尚、粉砕した肉類の平均粒子径は、特に限定されるものではないが、1μmを下回っていたり、1mmを上回っていたりすると、粉砕した肉類の間への処理液が浸透しすぎたり、逆に浸透しなかったりする虞があるため、1μm以上1mm以下であることが好ましい。 In the pulverizing step, for example, the meat after freeze-drying is pulverized in a mortar or the like to form powdery grains. The average particle size of the pulverized meat is not particularly limited, but if it is less than 1 μm or more than 1 mm, the treatment liquid may penetrate too much into the pulverized meat. It is preferable that the thickness is 1 μm or more and 1 mm or less because there is a possibility that the penetration may not occur.

浸透工程においては、具体的に、粉砕した肉類からなる粉粒体の任意の位置(例えば、粉粒体の全体又は一部、或いは表面全体又は表面の一部)に処理液を滴下して処理液を浸透させることで、処理液が浸透した部分を液状又はペースト状にする。粉粒体に処理液を滴下して浸透させる方法としては、所謂インクジェット方式を用いた方法を採用することが好ましい。即ち、浸透工程においては、処理液を微滴化(例えば1滴の体積が1pL以上1000pL以下)して粉粒体の任意の位置(例えば、全体又は一部、或いは表面全体又は表面の一部)に吹き付けて処理液を浸透させ、処理液が吹き付けられた部分を液状又はペースト状にすることが好ましい。この方法によれば、粉粒体の任意の位置に処理液を浸透させる際に、単に微滴化していない処理液を滴下するような場合と比較して、処理液を浸透させる位置を細かく調整できる。したがって、バリエーション豊かな形状の軟化食品を容易に製造することが可能になっている。尚、浸透工程に供する粉粒体は、無造作に置かれた状態であってもよいし、所定形状の型(容器)などの中に収容・充填された状態であってもよく、例えば、所定形状の型に収容・充填された状態であれば、粉粒体全体に処理液を浸透させて液状又はペースト状にした上で加熱工程を行うことにより、型通りの形状を有した軟化食品が得られる。 In the infiltration step, specifically, the treatment liquid is dripped onto an arbitrary position of the granules made of pulverized meat (for example, the whole or a part of the granules, or the whole surface or a part of the surface). By permeating the liquid, the portion permeated with the processing liquid becomes liquid or pasty. As a method of dripping and permeating the treatment liquid into the powder, it is preferable to adopt a method using a so-called ink-jet method. That is, in the permeation step, the treatment liquid is made into fine droplets (for example, the volume of one droplet is 1 pL or more and 1000 pL or less), and any position (for example, the whole or a part, or the whole surface or a part of the surface) of the powder or grain ) to allow the treatment liquid to permeate the surface, and the portion to which the treatment liquid has been sprayed is preferably liquid or pasty. According to this method, when the treatment liquid is permeated into an arbitrary position of the powder, the position to be permeated with the treatment liquid is finely adjusted compared to the case of simply dropping the treatment liquid that is not atomized. can. Therefore, it is possible to easily produce softened foods with a wide variety of shapes. In addition, the granular material to be subjected to the permeation step may be in a state of being placed casually, or may be in a state of being accommodated and filled in a mold (container) having a predetermined shape. If it is in a state of being accommodated and filled in a shape mold, the softened food having the shape of the mold is obtained by permeating the entire powder or granular material with the treatment liquid to make it liquid or pasty and then performing the heating process. can get.

また、浸透工程において使用する処理液としては、酵素を水やアルコールなどを含む水系の溶媒に分散させたものを用いることができる。処理液は、酵素の他、pH調整剤や調味料(塩、醤油、酒類、みりん、砂糖、ソース等)を含有していてもよいし、インクジェット方式を採用する場合には、ある程度の粘度が必要となることから、結着剤や界面活性剤を含有させることが好ましい。 As the treatment liquid used in the permeation step, a solution obtained by dispersing an enzyme in an aqueous solvent containing water or alcohol can be used. In addition to enzymes, the treatment liquid may contain pH adjusters and seasonings (salt, soy sauce, alcoholic beverages, mirin, sugar, sauce, etc.). Since it becomes necessary, it is preferable to contain a binder and a surfactant.

浸透工程で用いる処理液における酵素の濃度は、特に限定されるものではないが、0.001質量%未満であると、酵素の分解作用が弱くなり過ぎて処理に要する時間が増大する一方、1質量%より高いと、酵素自身の味が製造される軟化食品の味に強く現れすぎる、或いは、酵素の分解作用が強くなり過ぎて形状の維持が困難になる虞がある。したがって、処理液における酵素の濃度は、0.001質量%以上1質量%以下であることが好ましい。 The concentration of the enzyme in the treatment liquid used in the permeation step is not particularly limited, but if it is less than 0.001% by mass, the decomposition action of the enzyme becomes too weak and the time required for the treatment increases. If it is higher than % by mass, the taste of the enzyme itself may appear too strong in the taste of the softened food to be produced, or the decomposing action of the enzyme may become too strong, making it difficult to maintain the shape. Therefore, the concentration of the enzyme in the treatment liquid is preferably 0.001% by mass or more and 1% by mass or less.

使用する酵素としては、肉類中の任意の基質を分解することができるものであれば、特に限定されないが、基質がタンパク質である場合には、プロテアーゼを使用する。尚、プロテアーゼとしては、ブロメラインF(活性温度65~75℃)、パパインW-40(活性温度70~90℃)、アロアーゼ(登録商標)XA-10(活性温度60~75℃)、アロアーゼAP-10(活性温度50~65℃)、プロチンNY100(活性温度50~55℃)、プロチンSD-AY10(活性温度70~80℃)、ヌクレイシン(登録商標)(活性温度55~65℃)、アルカラーゼ(登録商標)、ビオプラーゼ(登録商標)OP(活性温度50~60℃)、ビオプラーゼSP-20(活性温度60~70℃)、オリエンターゼ(登録商標)22BF(活性温度60~70℃)、オリエンターゼAY(活性温度50~60℃)、オリエンターゼOP、プロテアーゼA「アマノ」SD(活性温度40~50℃)、プロテアーゼP「アマノ」SD(活性温度40~50℃)、プロテアーゼM「アマノ」G、スミチーム(登録商標)AP、スミチームACP-G、スミチームLP-G、スミチームFP-G、スミチームFLAP-G、スミチームDPP-G、デナプシン2P、デナチーム(登録商標)プロテアーゼYP-SS、デナチームAP(活性温度45~55℃)、デナチームPMC SOFTER(活性温度40~60℃)、パンチダーゼ(登録商標)NP-2(活性温度50~60℃)、サモアーゼ(活性温度50~60℃)、コラゲナーゼ(活性温度30~50℃)、ニューラーゼF3G(活性温度40~50℃)を例示することができる。また、酵素は、1種又は相互に阻害しないものを2種以上組み合わせて使用してもよい。更に、酵素は、植物由来であっても微生物由来であってもよく、植物由来のものと微生物由来のものとを組み合わせて使用してもよい。 The enzyme to be used is not particularly limited as long as it can decompose any substrate in meat, but protease is used when the substrate is protein. As the protease, Bromelain F (activity temperature 65-75°C), Papain W-40 (activity temperature 70-90°C), Alloase (registered trademark) XA-10 (activity temperature 60-75°C), Alloase AP- 10 (activity temperature 50-65°C), protin NY100 (activity temperature 50-55°C), protin SD-AY10 (activity temperature 70-80°C), Nucleisin (registered trademark) (activity temperature 55-65°C), Alcalase ( (registered trademark), Bioplase (registered trademark) OP (activity temperature 50-60°C), Biopase SP-20 (activity temperature 60-70°C), Orientase (registered trademark) 22BF (activity temperature 60-70°C), orientase AY (activity temperature 50-60°C), orientase OP, protease A "Amano" SD (activity temperature 40-50°C), protease P "Amano" SD (activity temperature 40-50°C), protease M "Amano" G , Sumizyme AP, Sumizyme ACP-G, Sumizyme LP-G, Sumizyme FP-G, Sumizyme FLAP-G, Sumizyme DPP-G, Denapsin 2P, Denazym® Protease YP-SS, Denazym AP (activity temperature 45-55°C), Denathym PMC SOFTER (activity temperature 40-60°C), pantydase (registered trademark) NP-2 (activity temperature 50-60°C), samoase (activity temperature 50-60°C), collagenase (activity temperature 30 to 50° C.) and Neurase F3G (activity temperature of 40 to 50° C.). Also, the enzymes may be used singly or in combination of two or more that do not inhibit each other. Furthermore, the enzyme may be plant-derived or microbial-derived, or a combination of plant-derived and microbial-derived enzymes may be used.

加熱工程においては、具体的に、処理液を浸透させた粉粒体を所定温度(例えば60℃)まで加熱し、その後、所定温度を一定時間(例えば30分)維持することで、酵素による分解反応によって基質(例えばタンパク質)を分解して肉類を軟化させるとともに、粉砕した肉類同士を結着させる。尚、肉類を所定温度に加熱して所定温度を一定時間維持する方法としては、処理液を浸透させた粉粒体をオーブンに入れ、60℃で30分間加熱する方法を例示できる。 Specifically, in the heating step, the granules infiltrated with the treatment liquid are heated to a predetermined temperature (eg, 60° C.), and then maintained at the predetermined temperature for a certain period of time (eg, 30 minutes), so that the particles are decomposed by enzymes. The reaction decomposes the substrate (eg protein) to soften the meat and bind the ground meat together. In addition, as a method of heating meat to a predetermined temperature and maintaining the predetermined temperature for a certain period of time, a method of putting powdery grains impregnated with the treatment liquid into an oven and heating at 60° C. for 30 minutes can be exemplified.

尚、本願において「肉類」とは、鶏肉や鴨肉、牛肉、馬肉、羊肉、猪肉などの鳥類・獣類の肉や、魚類や貝類などの魚介類の肉も含む概念である。 In the present application, "meat" is a concept including meat of birds and animals such as chicken, duck meat, beef, horse meat, mutton and wild boar meat, and seafood meat such as fish and shellfish.

[実施例及び比較例]
以下、具体的な実施例及び比較例について説明する。
[Examples and Comparative Examples]
Specific examples and comparative examples will be described below.

[実施例1]
サンプルとして牛モモ肉100gを使用し、サンプルを圧力鍋で30分間蒸して少なくとも100℃以上に加熱した後(前処理工程)、凍結乾燥装置を用いて2日間乾燥させた(凍結乾燥工程)。乾燥後のサンプルを乳鉢を用いて粉砕した(粉砕工程)。尚、粉砕したサンプル(粉砕した肉類からなる粉粒体)の平均粒子径について、走査型電子顕微鏡(日立製作所製のSU3500)の観察像から測長によって見積もったところ200μm程度であった。その後、粉砕した粉粒体0.25gを、底面が一辺の長さ30mmの正方形で深さが0.5mmの型に充填した。ついで、パパインW-40(天野エンザイム株式会社製)を0.1質量%となるように溶かした水(処理液)をインクジェット方式によって、上記粉粒体の上面から、直径20mmの円形の領域に、0.07ml塗布して処理液を浸透させた(浸透工程)。しかる後、サンプルをオーブンに入れ、60℃で30分間加熱した(加熱工程)。加熱後のサンプルについてテクスチャーメーター(株式会社山電製のRE2 33005B)で破断試験を行って硬さを測定した。また、形状について、任意の形状を有している場合には「良い」、有していない場合には「悪い」と評価し、食味について、食味の悪化が抑えられている場合には「良い」、抑えられていない場合には「悪い」と評価した。
[実施例2~4]
実施例2~4は、サンプルとして牛肉以外の肉(実施例2ではエビの肉、実施例3ではタコの肉、実施例4では鴨肉)を使用した点を除き、実施例1と同じ要領で行った。
[Example 1]
100 g of beef thigh was used as a sample, and the sample was steamed in a pressure cooker for 30 minutes and heated to at least 100 ° C. (pretreatment step), and then dried for 2 days using a freeze-drying apparatus (freeze-drying step). The dried sample was pulverized using a mortar (pulverization step). The average particle size of the pulverized sample (granules made of pulverized meat) was about 200 μm when estimated by length measurement from the observation image of a scanning electron microscope (SU3500 manufactured by Hitachi, Ltd.). After that, 0.25 g of the pulverized granular material was filled in a mold having a square base with a side length of 30 mm and a depth of 0.5 mm. Then, water (treatment liquid) in which 0.1% by mass of Papain W-40 (manufactured by Amano Enzyme Co., Ltd.) is dissolved is applied to a circular region with a diameter of 20 mm from the upper surface of the powder or granular material by an inkjet method. , 0.07 ml to permeate the treatment liquid (permeation step). After that, the sample was placed in an oven and heated at 60° C. for 30 minutes (heating step). The sample after heating was subjected to a rupture test using a texture meter (RE2 33005B manufactured by Yamaden Co., Ltd.) to measure hardness. Regarding the shape, it was evaluated as "good" if it had an arbitrary shape, and as "bad" if it did not. ', and 'bad' if not suppressed.
[Examples 2 to 4]
Examples 2 to 4 were the same as in Example 1, except that meat other than beef (shrimp meat in Example 2, octopus meat in Example 3, and duck meat in Example 4) was used as samples. I went with

[比較例1~4]
比較例1~4は、浸透工程において、酵素(パパインW-40及びアロアーゼ)を含まない処理液を使用した点を除き、実施例1~4と同じ要領で行った。尚、比較例1は牛モモ肉、比較例2はエビの肉、比較例3はタコの肉、比較例4は鴨肉を使用した。
[Comparative Examples 1 to 4]
Comparative Examples 1-4 were carried out in the same manner as Examples 1-4, except that a treatment solution containing no enzymes (papain W-40 and alloase) was used in the permeation step. Comparative Example 1 used beef thigh meat, Comparative Example 2 used shrimp meat, Comparative Example 3 used octopus meat, and Comparative Example 4 used duck meat.

[試験結果]
実施例1~4及び比較例1~3の硬さ、形状、食味を以下の表1にまとめた。表1中の「区分1」、「区分2」、「区分3」とは、軟化食品に関する指標の一つである、日本介護食品評議会が定める区分表における4つの区分の中に含まれるものである。具体的に、「区分1」とは「容易に噛める(硬さ上限値:500kPa)」、「区分2」とは「歯茎で潰せる(硬さ上限値:50kPa)」、「区分3」とは「舌で潰せる(硬さ上限値:20kPa)」と定義されている。
[Test results]
The hardness, shape and taste of Examples 1-4 and Comparative Examples 1-3 are summarized in Table 1 below. "Category 1", "Category 2", and "Category 3" in Table 1 are included in the four categories in the category table established by the Japan Nursing Care Food Council, which is one of the indicators for softened foods. is. Specifically, "Category 1" is "easy to chew (hardness upper limit: 500 kPa)", "Category 2" is "can be crushed with gums (hardness upper limit: 50 kPa)", and "Category 3" is It is defined as "can be crushed with the tongue (hardness upper limit: 20 kPa)".

Figure 0007330095000001
Figure 0007330095000001

実施例1~4と比較例1~4とを比較すると、形状については、サンプルの種類による違いは特に見られず、いずれの実施例及び比較例においても得られた軟化食品は任意の形状を有していた。一方、硬さについては、サンプルの違いによって差異はあるが、いずれのサンプルについても実施例の方が比較例よりも柔らかくなっており、実施例において得られた軟化食品は十分な柔らかさを備えたものとなっている。また、食味については、いずれのサンプルについても実施例において得られた軟化食品は食味が良く、比較例において得られた軟化食品は食味が悪かった。 When Examples 1 to 4 and Comparative Examples 1 to 4 are compared, there is no particular difference in shape depending on the type of sample, and the softened food obtained in any of Examples and Comparative Examples has an arbitrary shape. had. On the other hand, with regard to hardness, although there are differences depending on the difference in the samples, the examples are softer than the comparative examples for all samples, and the softened foods obtained in the examples have sufficient softness. It has become a thing. As for the taste, the softened foods obtained in the examples had a good taste, and the softened foods obtained in the comparative examples had a bad taste.

これらのことから、前処理工程、凍結乾燥工程、粉砕工程、浸透工程及び加熱工程を実施することによって、肉類を原材料とする軟化食品であって、十分な柔らかさを備えつつ、任意の形状を有し、食味の悪化が抑えられた軟化食品を製造できることが確認できた。 For these reasons, by performing the pretreatment process, freeze-drying process, pulverization process, permeation process and heating process, it is possible to obtain a softened food made from meat, which has sufficient softness and can be formed into any shape. It was confirmed that a softened food with reduced taste deterioration could be produced.

本発明の軟化食品製造方法は、肉類を原材料とする軟化食品を製造する際に用いることができる。 The method for producing a softened food product of the present invention can be used when producing a softened food product using meat as a raw material.

Claims (7)

肉類からなる軟化食品を製造する方法であって、
前記肉類を所定温度まで加熱する前処理工程と、
加熱した前記肉類を凍結乾燥する凍結乾燥工程と、
凍結乾燥した前記肉類を粉砕する粉砕工程と、
粉砕した前記肉類からなる粉粒体の少なくとも一部に、前記肉類中の所定の基質を分解する酵素を含む処理液を浸透させて、前記処理液が浸透した部位をペースト状にする浸透工程と、
前記処理液を浸透させた粉粒体を、前記酵素の活性温度まで加熱する加熱工程とを実施する軟化食品製造方法。
A method for producing a softened food consisting of meat,
A pretreatment step of heating the meat to a predetermined temperature;
a freeze-drying step of freeze-drying the heated meat;
A pulverizing step of pulverizing the freeze-dried meat;
A permeation step of infiltrating at least a part of the granules made of the pulverized meat with a treatment liquid containing an enzyme that decomposes a predetermined substrate in the meat , and turning the portion infiltrated with the treatment liquid into a paste. and,
and a heating step of heating the granules infiltrated with the treatment liquid to an activation temperature of the enzyme.
前記浸透工程において、前記粉粒体における任意の位置に前記処理液を微滴化して吹き付けて、前記粉粒体における任意の位置に前記処理液を浸透させる請求項1に記載の軟化食品製造方法。 2. The softened food production method according to claim 1, wherein in the permeation step, the treatment liquid is formed into fine droplets and sprayed onto an arbitrary position of the granular material to permeate the treatment liquid into an arbitrary position of the granular material. . 前記粉砕工程において、前記凍結乾燥した肉類を、平均粒子径が1μm以上1mm以下となるように粉砕する請求項1又は2に記載の軟化食品製造方法。 3. The method for producing a softened food according to claim 1 or 2, wherein in the pulverizing step, the freeze-dried meat is pulverized to have an average particle size of 1 μm or more and 1 mm or less. 前記前処理工程において、前記肉類を70℃以上に加熱する請求項1~3のいずれか一項に記載の軟化食品製造方法。 The softened food production method according to any one of claims 1 to 3, wherein the meat is heated to 70°C or higher in the pretreatment step. 前記酵素を含む処理液は、前記酵素の濃度が0.001質量%以上1質量%以下である請求項1~4のいずれか一項に記載の軟化食品製造方法。 The softened food manufacturing method according to any one of claims 1 to 4, wherein the treatment liquid containing the enzyme has a concentration of the enzyme of 0.001% by mass or more and 1% by mass or less. 前記酵素は、プロテアーゼである請求項1~5のいずれか一項に記載の軟化食品製造方法。 The method for producing softened food according to any one of claims 1 to 5, wherein the enzyme is protease. 前記前処理工程において、前記肉類を蒸すことで所定温度まで加熱する請求項1~6のいずれか一項に記載の軟化食品製造方法。 The softened food production method according to any one of claims 1 to 6, wherein in the pretreatment step, the meat is heated to a predetermined temperature by steaming.
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