JP7340290B2 - Anti-enveloped virus neutral detergent, disinfectant composition, and method for inactivating enveloped viruses - Google Patents

Description

The present invention relates to an anti-enveloped virus neutral detergent, a disinfectant composition containing the detergent, and a method for inactivating enveloped viruses using the same.

Currently, Japan and the international community are facing a serious risk of the rapid spread of the infectious disease (COVID-19) caused by the new coronavirus (SARS-CoV-2), and there is an urgent need to establish effective infectious disease control measures. ing.

There are three principles for infectious disease control: (1) measures against infected people, (2) measures against the source of infection, and (3) measures against the route of infection. However, when an infectious disease caused by a new virus occurs, many people do not have immunity, and it takes a considerable amount of time for each person to develop immunity through vaccines, etc., so it is difficult to prevent the spread of infection. (3) Measures against the infection route are important.

Measures to reduce the risk of contact infection include (i) washing off the virus on your hands with soap, (ii) disinfecting the virus on your hands with alcohol, and (iii) touching things with the virus on your fingers. (iv) Reduce the virus concentration on environmental surfaces by removing attached viruses by wiping, (v) Actively inactivate viruses on surfaces using products with virus inactivation effect, ( vi) There are known methods to avoid touching your mouth, nose, and eyes with contaminated hands. In the following, the removal of viruses attached to contact infection routes and the inactivation of viruses are also collectively referred to as "virus decontamination."

In such virus decontamination, inactivation of the virus is important in order to prevent unintentional spread of the virus. Therefore, various products (compositions) for inactivating enveloped viruses including SARS-CoV-2 are being investigated.

For example, quaternary ammonium compounds such as alkyldimethylbenzylammonium chloride (ADBAC) and didecyldimethylammonium chloride (DDAC) are known to be useful in a wide range of applications, including household and industrial disinfectant formulations ( Patent Document 1). On the other hand, their use in indirect food contact applications is limited to low levels due to regulations on maximum permissible use levels of these compounds. At such low levels, these quaternary ammonium compounds alone are typically not effective for their intended use.

Benzalkonium chloride and didecyldimethylammonium chloride have been reported to have many virus inactivating effects, mainly based on US FIFRA regulatory review data (Non-patent Documents 1 and 2).

It has been reported that benzalkonium chloride can inactivate SARS-CoV-2 by 3.8 Log10 or more at a concentration of 0.05% to 0.09%, and didecyldimethylammonium chloride can inactivate SARS-CoV-2 by 3.8 Log10 or more. A decrease of 4 Log10 was confirmed by 0.01% and 5 minutes of contact. However, there is no description of pH (Non-Patent Document 1). pH is an important factor, and Patent Document 2 describes that a quaternary ammonium salt cannot have a virus inactivating effect unless it is alkaline (pH 11 or higher) (Patent Document 2).
As mentioned above, quaternary ammonium salts are highly toxic to the skin and irritating to the eyes, so there is concern that their use alone may affect the human body.

In addition, cetylpyridinium chloride (CPC), a quaternary pyridinium salt, has a 99.95% reduction in feline infectious peritonitis type II (FIP type II) coronavirus after 5 minutes of exposure at a concentration of 0.025%. has been confirmed (Patent Document 3), and is expected to have a virus inactivating effect on SARS-CoV-2 (Non-Patent Document 3).

Although quaternary ammonium compounds (salts) are cationic surfactants, other surfactants are also being investigated for their ability to inactivate viruses.
For example, alkylamine oxide, which is an amphoteric surfactant, has an inactivating effect of 5 log 10 on SARS-CoV-2 at a concentration of 0.1% for 20 seconds, and a final concentration of 0.045% for 1 minute. It has been confirmed that inactivation exceeding 4log10 was achieved by contact with However, with respect to other amphoteric surfactants, there has been no report on the inactivating effect on the new coronavirus or influenza virus (Non-Patent Document 1).

Patent Document 4 describes that sulfobetaine and mixtures thereof, including alkylamine oxides, have an inactivating effect on enveloped viruses, but it is not clear how they inactivate the new coronavirus. Further, Patent Document 4 does not describe the combination of quaternary ammonium salt and sulfobetaine.
Rather, it is said that the combination of a quaternary ammonium salt and a betaine-type zwitterionic surfactant may reduce the bactericidal activity of the quaternary ammonium salt due to the ionic interaction of the surfactants (Patent Document 5).

As mentioned above, many anti-virus cleaning agents (anti-enveloped virus cleaning agents) that inactivate enveloped viruses such as SARS-CoV-2 have been studied, but they have a low impact on the human body and livestock and are virus-free. The emergence of a cleaning agent with high activation ability is desired.

Special Publication No. 2012-521434 JP 2019-052118 Publication Special Publication No. 2007-515204 Special table 2015-525572 publication Japanese Patent Application Publication No. 11-035974

Risk Studies Research, 2020, Volume 30, Issue 1, p. 5-28 Environ. Sci. Technol. Lett. 2020, 7, p. 622-631 Pharm. Res. 2020, 37:104

As mentioned above, there are problems in using quaternary ammonium salts alone on the human body, so how can we reduce the concentration of quaternary ammonium salts and reduce the impact on the human body to use them as anti-envelope virus agents? The question was whether it could be done.

The present invention has been made to solve the above problems, and provides an antiviral cleaning agent that has a low impact on the human body and has an inactive effect on enveloped viruses, a disinfectant composition containing the same, and a disinfectant composition containing the same. The purpose of this invention is to provide a method for inactivating enveloped viruses.

（式中、Ｒ_１～Ｒ_４は、直鎖状、分岐状又は環状のＣ１～Ｃ１８の炭化水素基であり、Ｒ_１とＲ_２は一緒になって環状になってもよい。Ｘはフッ素原子、塩素原子、臭素原子及びヨウ素原子から選択されるハロゲン原子、ヒドロキシ基、アルコキシ基、アシルオキシ基、アルキルスルホネート基、ジアルキルホスホネート基、アジペート基、メトサルフェート基、バイカーボネート基又はカーボネート基である。）
（Ｂ）両性界面活性剤としてのスルホベタイン：０．００３～３．０質量％ In order to solve the above problems, the present invention contains the following components (A) and (B) in the following proportions to the entire composition, and also contains water as the component (C), and Provided is an anti-envelope virus cleaning agent characterized by having a pH (JIS Z 8802:2011 "pH measurement method") of 6 to 8 at 25°C.
(A) Quaternary ammonium salt represented by the following general formula (1): 0.0001 to 0.4% by mass,

(In the formula, R ₁ to R ₄ are linear, branched, or cyclic C1 to C18 hydrocarbon groups, and R ₁ and R ₂ may be combined to form a ring. X is fluorine a halogen atom selected from a chlorine atom, a bromine atom, and an iodine atom, a hydroxy group, an alkoxy group, an acyloxy group, an alkylsulfonate group, a dialkylphosphonate group, an adipate group, a methosulfate group, a bicarbonate group, or a carbonate group. )
(B) Sulfobetaine as an amphoteric surfactant: 0.003 to 3.0% by mass

Such an anti-enveloped virus cleaning agent has little effect on the human body and has an inactivation effect against enveloped viruses.

In this case, R ₁ to R ₄ are preferably linear or branched C1 to C18 alkyl groups.

An anti-enveloped virus cleaning agent containing such component (A) has a higher inactivation effect on enveloped viruses.

（式中、Ｒ_５～Ｒ_７は置換されてもよいベンジル基、又は分岐してもよいＣ１～Ｃ１８のアルキル基であり、Ｒ_５とＲ_６が一緒になって環状になってもよい。ＹはＣ１からＣ５のアルキレン基である。ただし、ＹがＣ３のアルキレン基である場合は２位に置換基が入ってもよい。） In the anti-envelope virus cleaning agent, the sulfobetaine is preferably represented by the following general formula (2).

(In the formula, R ₅ to R ₇ are optionally substituted benzyl groups or optionally branched C1 to C18 alkyl groups, and R ₅ and R ₆ may be combined to form a ring. Y is a C1 to C5 alkylene group. However, if Y is a C3 alkylene group, a substituent may be present at the 2-position.)

Such component (B) has a synergistic effect with component (A), thereby further increasing the inertness effect.

In the anti-envelope virus cleaning agent, the quaternary ammonium salt is preferably a dialkyldimethylammonium salt.

It is particularly preferable that the anti-enveloped virus cleaning agent of the present invention contains such component (A).

In the anti-envelope virus cleaning agent, the sulfobetaine is preferably lauryldimethyl-2-hydroxypropyl-3-sulfobetaine.

It is particularly preferable that the anti-enveloped virus cleaning agent of the present invention contains such component (B).

In the above-mentioned anti-enveloped virus cleaning agent, target viruses include influenza virus, new coronavirus (SARS-CoV-2) and its mutant strains, avian influenza virus, SFTS virus (severe fever thrombocytopenia syndrome), Ebola virus, coronavirus ( MERS, SARS), dengue fever, Zika fever, swine fever virus (CSFV), and ASFvirus (African swine fever).

The anti-enveloped virus cleaning agent of the present invention has a high ability to inactivate such viruses.

In this case, the target virus is the new coronavirus (SARS-CoV-2) and its mutant strains, and the component (A) is contained in a proportion of 0.0001 or more and less than 0.01% by mass based on the entire composition. It is particularly preferable that the

Such an anti-enveloped virus cleaning agent has particularly high inactivation ability against the new coronavirus and its mutant strains.

The present invention also provides a disinfectant composition comprising the above-mentioned anti-enveloped virus detergent.

Such a disinfectant composition has little effect on the human body and has an inactivating effect against enveloped viruses.

The present invention also provides a method for inactivating enveloped viruses, which comprises bringing the anti-enveloped virus cleaning agent or disinfectant composition into contact with enveloped viruses to inactivate the enveloped viruses. .

The method for inactivating enveloped viruses of the present invention has a low impact on the human body and has a high ability to inactivate enveloped viruses, so it can be used more effectively without causing unintentional spread of the virus. Efficient and sufficient virus inactivation is possible.

According to the present invention, the combination of at least two low-concentration surfactants in a neutral region (pH = 6-8) has little effect on the human body and livestock, and has a high ability to inactivate enveloped viruses. Since it is possible to provide an anti-enveloped virus cleaning agent and disinfectant composition, it is effective in inactivating enveloped viruses such as the new coronavirus and its mutant strains, and has extremely high utility value as a countermeasure against infectious diseases.

As mentioned above, there has been a need for the development of an antiviral cleaning agent that has less impact on the human body and has an inactivating effect on enveloped viruses.

As a result of intensive studies on the above-mentioned problems, the present inventors found that other surfactants (ampholytic surfactants: betaine (carbobetaine, sulfobetaine, phosphobetaine), alkyl glucosides, alkyl amine oxides, We have completed the present invention by discovering that several types of cocktail detergents that combine detergents (e.g.) at specific concentrations are effective, and among these, sulfobetaine synergistically increases the antiviral properties of ammonium cations.

（式中、Ｒ_１～Ｒ_４は、直鎖状、分岐状又は環状のＣ１～Ｃ１８の炭化水素基であり、Ｒ_１とＲ_２は一緒になって環状になってもよい。Ｘはフッ素原子、塩素原子、臭素原子及びヨウ素原子から選択されるハロゲン原子、ヒドロキシ基、アルコキシ基、アシルオキシ基、アルキルスルホネート基、ジアルキルホスホネート基、アジペート基、メトサルフェート基、バイカーボネート基又はカーボネート基である。）
（Ｂ）両性界面活性剤としてのスルホベタイン：０．００３～３．０質量％ That is, the present invention contains the following components (A) and (B) in the following proportions to the entire composition, and also contains water as the component (C), and the pH of the composition (JIS Z 8802: It is an anti-enveloped virus cleaning agent characterized by having a pH of 6 to 8 at 25°C (2011 "pH Measurement Method").
(A) Quaternary ammonium salt represented by the following general formula (1): 0.0001 to 0.4% by mass,

(In the formula, R ₁ to R ₄ are linear, branched, or cyclic C1 to C18 hydrocarbon groups, and R ₁ and R ₂ may be combined to form a ring. X is fluorine a halogen atom selected from a chlorine atom, a bromine atom, and an iodine atom, a hydroxy group, an alkoxy group, an acyloxy group, an alkylsulfonate group, a dialkylphosphonate group, an adipate group, a methosulfate group, a bicarbonate group, or a carbonate group. )
(B) Sulfobetaine as an amphoteric surfactant: 0.003 to 3.0% by mass

Hereinafter, the present invention will be explained in detail, but the present invention is not limited thereto.

<Anti-envelope virus cleaning agent>
An object of the present invention is to provide an anti-enveloped virus cleaning agent (anti-enveloped virus neutral cleaning agent) or a disinfectant composition. Furthermore, by using two or more types of surfactants as active ingredients, the diluted surfactants exhibit anti-enveloped virus activity and are environmentally friendly.

As mentioned above, the present invention has completed the present invention of an anti-enveloped virus cleaning or disinfecting composition in the neutral region.
The anti-envelope virus cleaning agent of the present invention is a composition that contains components (A) and (B), which will be described later, in a specific ratio to the entire composition, and water as the component (C). The pH of the composition is 6 to 8 at 25°C. In this specification, pH refers to a value measured according to JIS Z 8802:2011 "pH measurement method", and neutral refers to a pH range of 6 to 8.
In addition to the above-mentioned (A) to (C) components, the anti-enveloped virus cleaning agent of the present invention further contains additives such as surfactants other than the above-mentioned (A) and (B) components, if necessary. You can also do that. Each component will be explained below.

（式中、Ｒ_１～Ｒ_４は、直鎖状、分岐状又は環状のＣ１～Ｃ１８の炭化水素基であり、Ｒ_１とＲ_２は一緒になって環状になってもよい。Ｘはフッ素原子、塩素原子、臭素原子及びヨウ素原子から選択されるハロゲン原子、ヒドロキシ基、アルコキシ基、アシルオキシ基、アルキルスルホネート基、ジアルキルホスホネート基、アジペート基、メトサルフェート基、バイカーボネート基又はカーボネート基である。） [(A) Quaternary ammonium salt]
Component (A) is a quaternary ammonium salt represented by the following general formula (1).

(In the formula, R ₁ to R ₄ are linear, branched, or cyclic C1 to C18 hydrocarbon groups, and R ₁ and R ₂ may be combined to form a ring. X is fluorine a halogen atom selected from a chlorine atom, a bromine atom, and an iodine atom, a hydroxy group, an alkoxy group, an acyloxy group, an alkylsulfonate group, a dialkylphosphonate group, an adipate group, a methosulfate group, a bicarbonate group, or a carbonate group. )

In the general formula (1), R ₁ to R ₄ are linear, branched, or cyclic C1 to C18 hydrocarbon groups, and R ₁ and R ₂ may be combined to form a ring. The number of carbon atoms in the hydrocarbon group can be, for example, 8 or more and 15 or less for R ₁ and R ₂ , and 1 or more and 5 or less for R ₃ and R ₄ . Specific examples of the hydrocarbon group include an alkyl group, an alkenyl group such as an allyl group, an alkynyl group, and a cycloalkyl group.
Note that "C1" and "C18" indicate that the number of carbon atoms is 1 and 18, respectively. The same applies below.

Specific examples of C1 to C18 hydrocarbon groups include C1 to C18 straight chain alkyl groups such as methyl, ethyl, and propyl groups; C1 to C18 straight chain alkenyl groups such as vinyl and allyl groups; and ethynyl groups. Examples include straight chain alkynyl groups such as, C3 to C18 branched alkyl groups such as isopropyl groups, C3 to C18 branched alkenyl groups such as isopropenyl groups, and C3 to C18 cyclic alkyl groups such as cyclohexyl groups. Preferably, R ₁ to R ₄ are linear or branched C1 to C18 alkyl groups. More preferably, it is a linear C1 to C18 alkyl group, and particularly R ₃ and R ₄ are preferably a methyl group or an ethyl group.

X is a halogen atom selected from a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom, a hydroxy group, an alkoxy group, an acyloxy group, an alkylsulfonate group, a dialkylphosphonate group, an adipate group, a methosulfate group, a bicarbonate group, or a carbonate group It is. Although these groups are not particularly limited, examples of the hydrocarbon groups included in these groups include those similar to R ₁ to R ₄ above. Since X is not involved in virus inactivation, any X can be used as long as it forms a salt.

The quaternary ammonium salt of component (A) is not particularly limited as long as it is a quaternary ammonium salt represented by the above general formula (1), but is preferably a dialkyldimethylammonium salt, and a didecyldimethylammonium salt. It is more preferable that The dialkyldimethylammonium salt may have an alkyl group of C1 to C18, and may be a mixture of two or more different dialkyldimethylammonium salts. Such a dialkyldimethylammonium salt contains a DDAC analog and may be a commercially available product, such as Biocide ST-70H (manufactured by Taisho Technos), which is commercially available as a DDAC analog cation disinfectant.
If component (A) is a quaternary ammonium salt that is different from the quaternary ammonium salt represented by general formula (1) above, the synergistic effect of component (B) described below will not be expressed, and it will not be effective against enveloped viruses. Poor activation ability.

The concentration of component (A) is from 0.0001% by mass to 0.4% by mass, preferably from 0.0002 to 0.007% by mass. Regarding the new coronavirus (SARS-CoV-2) and its mutant strains, it can be less than 0.01% by mass. If the concentration of component (A) is less than 0.0001% by mass, the inactivating effect on enveloped viruses will be insufficient. Non-patent Document 1 states that 0.01% by mass or more is effective against the new coronavirus (SARS-CoV-2), but the environment (temperature, pH), etc. is not clear, and in some cases it may be harmful to the human body. There are concerns about the impact of Moreover, if the mass exceeds 0.4, the influence on the human body will be greater.

[(B) Amphoteric surfactant]
Component (B) is sulfobetaine as an amphoteric surfactant. In this specification, sulfobetaine is defined as having a positive charge and a negative charge at non-adjacent positions in the same molecule, and no dissociable hydrogen atom is bonded to the positively charged atom (a cation such as quaternary ammonium Structure) refers to a compound that is electrically neutral as a whole and has a sulfonate group. Although sulfobetaine can also have a quaternary ammonium structure, it is a compound clearly different from component (A) in that it has a sulfonate group.
There is no report in Non-Patent Document 1 regarding the inactivating effect of component (B) on enveloped viruses by itself, but when combined with the quaternary ammonium salt of component (A), the quaternary ammonium salt can inactivate enveloped viruses. It exhibits a synergistic effect of improving the inertness effect on. In the present invention, by combining components (A) and (B), it is possible to reduce the concentration of quaternary ammonium salts, which are problematic for use on the human body when used alone, and to reduce the effect on the human body. It can be used as a virus cleaning agent.

（式中、Ｒ_５～Ｒ_７は置換されてもよいベンジル基、又は分岐してもよいＣ１～Ｃ１８のアルキル基であり、Ｒ_５とＲ_６が一緒になって環状になってもよい。ＹはＣ１からＣ５のアルキレン基である。ただし、ＹがＣ３のアルキレン基である場合は２位に置換基が入ってもよい。） The chemical structure of the component (B), sulfobetaine, is not particularly limited, but is preferably represented by the following general formula (2).

(In the formula, R ₅ to R ₇ are optionally substituted benzyl groups or optionally branched C1 to C18 alkyl groups, and R ₅ and R ₆ may be combined to form a ring. Y is a C1 to C5 alkylene group. However, if Y is a C3 alkylene group, a substituent may be present at the 2-position.)

R ₅ to R ₇ are optionally substituted benzyl groups or optionally branched C1 to C18 alkyl groups, and R ₅ and R ₆ may be taken together to form a ring. Specific examples include the groups shown above for R ₁ to R ₄ . The benzyl group may be substituted with an alkyl group, an alkoxy group, a hydroxyl group, a cyano group, a halogen atom, or the like.

Y is a C1 to C5 alkylene group. However, when Y is a C3 alkylene group (trimethylene group), a substituent may be present at the 2-position. Examples of the substituent include those shown for the benzyl group above. Specific examples of Y include methylene group, ethylene group, trimethylene group, tetramethylene group, pentamethylene group, and 2-hydroxypropyl group.
By appropriately selecting R ₅ to R ₇ , the balance between hydrophobicity and hydrophilicity of sulfobetaine can be adjusted, and by appropriately selecting Y, the distance between positive and negative charges can be adjusted. By adjusting these according to the component (A) to be combined, a desirable synergistic effect (inactivation effect against enveloped viruses) can be exerted.

Among them, it is preferable that the sulfobetaine is lauryldimethyl-2-hydroxypropyl-3-sulfobetaine. Such component (B) has a synergistic effect with component (A), thereby further increasing the inertness effect. This is considered to be because the sulfobetaine has an appropriate hydrophobic group (lauryl group), a hydrophilic group (hydroxyl group), and an appropriate distance between positive charges and negative charges (C3 group).

The component (B), sulfobetaine, may be a commercially available product. For example, Amhitol 20HD (manufactured by Kao), which is an amphoteric surfactant with little skin irritation and is used in shampoos and the like, can be mentioned.

The concentration of component (B) is 0.003 to 3.0% by mass, preferably 0.01 to 0.3% by mass. If the concentration of component (B) is less than 0.003% by mass, the synergistic effect when combined with component (A) will be insufficient, and if it exceeds 3.0% by mass, the synergistic effect will not change but the cost will increase. This is not desirable.

[(C) Water]
Examples of the water (component (C)) used in the cleaning agent of the present invention include tap water, hard water, soft water, ion exchange water, pure water, purified water, and the like. From the viewpoint of storage stability of the composition, it is preferable to use soft water, ion-exchanged water, pure water, purified water, etc. However, as described later, the cleaning agent of the present invention has sufficient water resistance even if the dilution water is hard water. Antiviral activity can be maintained.

In the cleaning agent of the present invention, water is blended in an amount necessary for the total amount with other components to be 100% by mass (so-called balance).

[(D) Additive]
The anti-enveloped virus cleaning agent of the present invention may contain surfactants, chelating agents, water-soluble solvents, etc. other than the components (A) and (B), if necessary.

(surfactant)
The surfactant as component (D) is not particularly limited as long as it is other than components (A) and (B), but for example, cationic surfactant other than component (A), anionic surfactant Examples include surfactants, amphoteric surfactants other than component (B), and nonionic surfactants. Among these, nonionic surfactants having wetting, penetrating, cleaning, emulsifying, and dispersing functions are preferred. Examples of nonionic surfactants include polyoxyalkylene alkyl ethers such as polyoxyethylene alkyl ether and polyoxypropylene alkyl ether, copolymers such as polyoxyethylene oxypropylene block polymers, alkyl glycosides, nonylphenyl ethers, etc. Examples include alkylphenyl ethers and carboxylic acid esters. Specific examples of surfactants include those disclosed in Patent Document 2, for example. In the present invention, polyoxyethylene alkyl ether is particularly preferred. As the polyoxyethylene alkyl ether, commercially available products such as Neugen TDX80D (manufactured by Dai-ichi Kogyo Seiyaku) may be used.
The concentration of the surfactant in the detergent can be from 0.002 to less than 0.2% by mass.

(chelating agent)
As the chelating agent, those commonly used in antibacterial cleaning agents can be used, and examples thereof include at least one selected from hydroxycarboxylic acids and salts thereof, and aminocarboxylic acids and salts thereof. Examples of hydroxycarboxylic acids include citric acid, malic acid, gluconic acid, succinic acid, tartaric acid, lactic acid, etc.; examples of aminocarboxylic acids include methylglycine diacetic acid, ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid, and diethylenetriaminepentaacetic acid. , hydroxyethylethylenediaminetetraacetic acid, and the like. Examples of these salts include alkali metal salts, alkaline earth metal salts, ammonium salts, and alkanolamine salts. Aminocarboxylic acids or salts thereof are preferred from the viewpoint of availability, detergency, and storage stability. In the composition of the present invention, the chelating agents selected from the above may be used alone or in combination of two or more. For example, in the cleaning agent of the present invention, ethylenediaminetetraacetic acid (EDTA) can be suitably used.

In the cleaning agent of the present invention, the amount of the chelating agent can be set in the range of 0.1 to 5% by weight, preferably 0.5 to 3% by weight. If the amount of chelating agent is small, the cleaning effect and storage stability of the cleaning agent may be poor. On the other hand, if the amount of chelating agent is too large, the cleaning power improvement effect derived from the chelating agent will be saturated. , it may actually be economically disadvantageous.

The cleaning agent of the present invention contains a surfactant such as a nonionic polymer polyether that stabilizes cations and a chelating agent such as EDTA, so that the solution can be prevented from becoming cloudy even at low temperatures. . Furthermore, by adding nonionic polymer polyether, etc., the antiviral activity can be maintained regardless of whether the water to be diluted is soft water or hard water due to its chelating effect.

(Water-soluble solvent)
As the water-soluble solvent, those commonly used for antibacterial cleaning agents can be used, and examples thereof include at least one selected from glycol ether solvents and alcohols. Specific examples of water-soluble solvents include those disclosed in Patent Document 2, for example.

In the cleaning agent of the present invention, water-soluble solvents selected from the above may be used alone, or two or more types may be used in combination.

The cleaning agent of the present invention may contain a water-soluble solvent in an amount set in the range of 0 to 20% by weight, preferably 5 to 15% by weight, and more preferably 7 to 10% by weight.

(Other ingredients)
In addition, the cleaning agent of the present invention may further contain other additives as necessary, such as skin irritation relievers, dyes, fragrances, preservatives, metal corrosion inhibitors, hop extracts, and disinfectants such as polylysine. can do.

<Preparation of anti-envelope virus cleaning agent>
The cleaning agent of the present invention is generally prepared by mixing and stirring each component when it is a liquid, or by first dissolving it in water and then adding other liquid components and mixing and stirring when it contains solid components. However, depending on the composition, the order of addition of each component, the order of dissolution, and heating /
Manufacturing procedures such as cooling are not particularly limited.

The pH of the anti-enveloped virus cleaning agent of the present invention (JIS Z 8802:2011 "pH measurement method") is 6 to 8 (neutral range) at 25°C. In the present invention, by setting the pH of the cleaning agent within the above range, it is possible to reduce the influence on the human body, and also to have an inactivating effect on enveloped viruses.
The pH is adjusted using an alkaline agent such as an alkali metal hydroxide, carbonate, bicarbonate, or carboxylate, preferably an alkali metal hydroxide, more preferably sodium hydroxide and/or potassium hydroxide. It can be adjusted within the above range.

The anti-envelope virus cleaning agent (composition) of the present invention may be used after being appropriately diluted, for example, 2 to 1000 times. Dilution can be performed using water (hardness 0 to 1000 (unit: mg/L, same hereinafter)).

<Target virus>
The target virus of the anti-enveloped virus cleaning agent of the present invention is not particularly limited, and enveloped viruses can be targeted. For example, the new coronavirus (SARS-CoV-2) and its mutant strains, influenza viruses (types A, B, and C), avian influenza virus, SFTS virus (severe fever thrombocytopenia syndrome), Ebola virus, coronavirus (MERS), , SARS, etc.), dengue fever, Zika fever, swine fever virus (CSFV), ASFvirus (African swine fever), yellow fever virus, Japanese encephalitis virus, St. Louis encephalitis, Murray Valley encephalitis, West Nile fever, Central European encephalitis, Russian spring/summer Encephalitis, hepatitis B virus, hepatitis C virus, hepatitis D virus, rubella virus, Sindbis virus, chikungunya virus, eastern equine encephalitis virus, western equine encephalitis virus, Venezuelan equine encephalitis virus, human T lymphotropic virus, Human immunodeficiency virus (HIV), Marburg virus, rabies virus, California encephalitis virus, Hantan virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, Togoto virus, Dori virus, parainfluenza virus, mump virus, measles virus, Respiratory syncytial virus, Lassa virus, lymphocytic choriomeningitis virus, Hunni virus, Machupo virus, Guanarito virus, human herpesvirus, human herpesvirus 2 to 8, variola virus, bovine viral diarrhea virus 1 and 2, border Examples include disease viruses 1, 2, and 3, giraffe pestivirus, and the like. Among them, the target viruses are influenza virus, new coronavirus (SARS-CoV-2) and its variants, avian influenza virus, SFTS virus (severe fever thrombocytopenia syndrome), Ebola virus, coronavirus (MERS, SARS), The anti-enveloped virus cleaning agent of the present invention exhibits excellent virus inactivation ability against dengue fever, Zika fever, swine fever virus (CSFV), and ASFvirus (African swine fever).
The anti-enveloped virus cleaning agent of the present invention has a low effect on the human body and livestock, and has a high ability to inactivate enveloped viruses such as those described above. Therefore, it is effective in decontaminating enveloped viruses, and has extremely high utility value as a countermeasure against infectious diseases in humans and livestock.

<Disinfectant composition>
The disinfectant composition of the present invention contains the above-mentioned anti-enveloped virus cleaning agent.
In addition to the above-mentioned anti-enveloped virus cleaning agent, the disinfectant composition can optionally contain the above-mentioned additives and the like.

<Virus inactivation method>
The present invention also provides a method for inactivating enveloped viruses, which comprises bringing the anti-enveloped virus cleaning agent of the present invention or the disinfectant composition of the present invention into contact with enveloped viruses to inactivate the enveloped viruses. provide.
The above-mentioned anti-envelope virus cleaning agent or disinfectant composition (hereinafter also collectively referred to as "composition of the present invention") has a low concentration of the quaternary ammonium salt of the component (A), so it is harmful to the human body, etc. impact is low. On the other hand, since the coexisting component (B), sulfobetaine, synergistically improves the antiviral properties of ammonium cations, the composition of the present invention has the ability to inactivate enveloped viruses including the new coronavirus. expensive.
Since enveloped viruses are inactivated using the composition of the present invention, more efficient and sufficient virus inactivation can be achieved without causing unintentional spread of the virus.

An object to be cleaned using the composition of the present invention can be cleaned by the following method.
(1) The composition of the present invention can be impregnated into a nonwoven fabric to make an antiviral cleaning material (hereinafter sometimes referred to as "the antiviral cleaning material of the present invention"), and this can be used to inactivate viruses. By performing the wiping operation on the object to be cleaned, the virus can be inactivated and the object to be cleaned can be cleaned. Items to be cleaned include cooking tables, refrigerators, and storage areas in medical facilities, nursing care facilities, food factories, coffee shops, restaurants, hotels, bars, schools (school lunches), central kitchens, supermarket backyards, etc., as well as tables, Examples include hard surfaces such as desks, doorknobs at entrances and exits, toilet bowls and seats, water faucets, and floors and walls. Furthermore, the antiviral cleaning material of the present invention can be used as wet towels, wet tissues, and the like. In particular, according to the antiviral cleaning material of the present invention, even if the object to be cleaned is contaminated with organic dirt, the virus can be inactivated and the object to be cleaned can be inactivated by wiping without reducing or losing the virus inactivation effect. can be cleaned. The antiviral cleaning material of the present invention can be used, for example, for cleaning livestock barns or sprayed on the street.

Examples of raw materials for the nonwoven fabric constituting the antiviral cleaning material of the present invention include cellulose materials such as cotton, protein materials such as wool or silk, and chemically polymerized materials such as rayon, polyester, and acrylic. Among these, cellulose materials and chemical polymerization materials are preferred.

(2) The composition of the present invention can be used by other methods as appropriate in addition to those described above. For example, it may be sprayed onto the surface of the object to be cleaned, left for a predetermined period of time, rinsed with water as appropriate, and then dried. Specifically, a dedicated dispenser containing the composition of the present invention is used to spray the surface of the object to be cleaned each time it is used, thereby inactivating the virus and cleaning the object. be able to. The objects to be cleaned include those mentioned above.

The composition of the present invention is provided by filling a plastic container, a container with a pump, a pouch, a tube, or the like. Moreover, it can also be provided in a corresponding amount to be used each time, individually packaged and provided with portability.

Hereinafter, the present invention will be specifically explained using Examples and Comparative Examples, but the present invention is not limited thereto.
In addition, in the following, pH is a value measured at 25°C according to JIS Z 8802:2011 "pH measurement method". Furthermore, the ability to inactivate enveloped viruses was evaluated using influenza virus (H3N2) and new coronavirus as enveloped viruses.

(Example 1-2, Comparative Example 1-5)
According to the composition in Table 1 below, each component was mixed in the proportions of sulfobetaine (10 wt% as Amhitol 20HD), water (89.5 wt%), and a test sample (0.5 wt%), and the antiviral cleaning agent composition of each composition was determined. I prepared something. All were colorless and transparent. A regulator (NaOHaq) was used for pH adjustment. In the table, the amount of each component is shown in mass %. In the table, Amhitol 20HD (Kao product) is sulfobetaine, specifically lauryldimethyl-2-hydroxypropyl-3-sulfobetaine (see formula below). Amhitol 20HD contains 30% by mass of the sulfobetaine.

Each antiviral cleaning agent composition was diluted 100 times with water (hardness 70) to prepare an antienvelope virus cleaning agent, which was used in an anti-influenza test.

The anti-influenza test (plaque method) was conducted on influenza virus (H3N2) with an action time of 10 minutes. A regulator (NaOHaq) was used for pH adjustment. Regarding the effectiveness of the inactivation ability obtained by the plaque method, ◎: sufficient effect (4 Log 10 or more), ○: effective (2 Log 10 or more and less than 4 Log 10), ×: no effect (less than 2 Log 10).

表中の４級ピリジニウム塩は、塩化セチルピリジニウムである。

The quaternary pyridinium salt in the table is cetylpyridinium chloride.

As shown in Table 1, the anti-enveloped virus cleaning agent of the present invention contains a specific quaternary ammonium salt and sulfobetaine at a specific concentration, so that the two exhibit a synergistic effect, resulting in a quaternary Even at low concentrations, ammonium salts have sufficient ability to inactivate enveloped viruses. Furthermore, since the concentration of quaternary ammonium salt is low, it has little effect on the human body.
On the other hand, in Comparative Examples 1-3 containing quaternary pyridinium salts that do not correspond to component (A) of the present invention and quaternary ammonium salts having a benzyl group, and Comparative Example 4 containing no quaternary ammonium salts, enveloped viruses were inactivated. was not effective. Furthermore, although the commercially available product (Comparative Example 5) is effective in inactivating enveloped viruses, it has a high pH and has a large impact on the human body. Although the anti-enveloped virus detergent of Comparative Example 1-4 contained sulfobetaine, it was not effective in inactivating enveloped viruses, so even if sulfobetaine was present alone, it did not inactivate enveloped viruses. It can be inferred that it has no effect.

(Example 3-8)
According to the composition of Table 2 below, each component was mixed in the same manner as in Example 1, except that Neugen TDX80D (manufactured by Daiichi Kogyo Seiyaku) was used as the polyoxyethylene alkyl ether, and an antiviral cleaning composition of each composition was prepared. Prepared. All were colorless and transparent. A regulator (NaOHaq) was used for pH adjustment. In the table, the amount of each component is shown in mass %.

Each antiviral cleaning agent composition was diluted 100 times with water (hardness 70) to prepare an antienvelope virus cleaning agent, which was used in an anti-influenza test. The anti-influenza test (plaque method) was performed on influenza virus (H3N2) with an action time of 5 minutes. The results were evaluated in the same manner as above.

As shown in Table 2, the anti-enveloped virus detergent of the present invention has sufficient ability to inactivate enveloped viruses at the sulfobetaine concentration of the present invention. Further, even if the quaternary ammonium counter anion is a chloride ion or a bromide ion, it has a sufficient ability to inactivate enveloped viruses.

(Example 9)
Antiviral cleaning by mixing didecyldimethylammonium chloride (0.4g), Amhitol 20HD (10.0g), Neugen TDX80D (2.0g), EDTA (0.2g), and water (87.4g, hardness 70). A drug composition was prepared. All were colorless and transparent. A regulator (NaOHaq) was used for pH adjustment (pH=7.2).
The composition of the antiviral cleaning composition is as follows.
Part by mass Didecyldimethylammonium chloride 0.4
Sulfobetaine 3.0
Poly(oxyethylene)=alkyl ether 2.0
EDTA 0.2
water remainder
Total 100

The anti-virus cleaning composition was diluted 100 times with water (natural mineral water: Evian (registered trademark), (Kasha water source: hardness 304)) to prepare an anti-envelope virus cleaning composition, which was used in an anti-influenza test. The effectiveness of the inactivation ability obtained by the plaque method was evaluated in the same manner as above while changing the exposure time (action time). The results are shown in Table 3.

The anti-enveloped virus cleaning agent of the present invention exhibits sufficient ability to inactivate enveloped viruses with an exposure time of 1 minute even if the 100-fold concentrated solution is diluted with water with a hardness of 304, and even after that, the anti-enveloped virus cleaning agent shows sufficient ability to inactivate enveloped viruses over time. Activation ability can be maintained. This means that the compact, easy-to-transport 100-fold concentrated solution maintains sufficient antiviral activity even when diluted with highly hard water from hot spring areas or overseas (Las Vegas, USA, Munich, Germany, Beijing, China, etc.) It means that.
Dialkyl-type quaternary ammonium salts such as dialkyldimethylammonium salts are less susceptible to the effects of hard water and have superior bactericidal performance, making them more widely used than conventional cationic surfactant-type bactericidal agents (Patent Document 5). This also supports the results of the above test (effects of the present invention).

(Example 10)
A skin irritation test was conducted on the anti-envelope virus cleaning agent used in Example 9.
The test is carried out according to the protocol of the skin irritation test set (OECD TG 439; In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method) to determine the cell viability due to the anti-enveloped virus detergent (surfactant formulation).

I-1. Preparation of test solution Positive control: 500 mg of SLS (sodium lauryl sulfate) was weighed and sterile distilled water was added to make 10 mL.
Negative control; sterile distilled water, phosphate buffer; 50mM phosphate buffer (pH 7.2) was prepared and filled into a sterilized polyethylene washing bottle.
MTT medium; MTT reagent (6 mg) was dissolved in assay medium (12 mL). Note that MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] is a pale yellow substrate that is cleaved by the mitochondria of living cells to produce bright blue formazan (dead). (not cleaved in cells). The amount of formazan produced correlates with the number of living cells.
Test solution (test substance): anti-envelope virus cleaning agent prepared in Example 9

I-2. Test procedure The assay medium was warmed in a 37°C water bath. The assay medium was dispensed in 0.5 mL portions onto the first row of four assay plates (4 rows x 6 columns). The culture cup was removed from the LabCyte EPI-Model and transferred to the well of the assay plate into which the assay medium had been dispensed. The assay plate was placed in a sealed container containing a carbon dioxide gas culture pack, and cultured in a 25°C incubator for 18 hours. The assay medium, which had been heated to 37° C. in a water bath, was dispensed in 1.0 mL portions onto the third row of the assay plate taken out from the incubator. The assay plate was placed in a sealed container containing a carbon dioxide gas culture pack, and cultured for 3 hours in an incubator at 25°C. 25 μL of the test substance was pipetted and dropped onto the surface of the cultured epidermis in the first row (n=3). After 15 minutes of exposure to the test substance, the culture cup was removed with tweezers, and the inside of the cup was washed with phosphate buffer. After repeating the washing 15 times, excess phosphate buffer solution was wiped off the outside of the cup with a sterile cotton swab. The cup containing the washed cultured epidermis was transferred to the culture medium in the third row. The assay plate after culture was taken out from the incubator, and 0.5 mL of MTT medium warmed in a 37° C. water bath was dispensed into the wells in the fourth row. The assay plate was placed in a sealed container containing a carbon dioxide gas culture pack, and cultured for 42 hours in an incubator at 25°C. Cultured epidermis was removed from row 3 and transferred to wells in row 4 containing MTT medium. The assay plate was placed in a sealed container containing a carbon dioxide gas culture pack, and cultured for 3 hours in a 25°C incubator. After the reaction, the cultured epidermis in each well in the fourth row was picked up with tweezers and placed in a 1.5 mL microtube. 300 μL of isopropanol was added to completely immerse the cultured epidermis. The dye was extracted by leaving it in the refrigerator for 48 hours, and the absorbance of the MTT reaction extract at 570 nm and 650 nm was measured. The viable cell rate was calculated by applying the following formula.

Measured value = [Absorbance of sample (570 nm) - Absorbance of sample (650 nm)] - [Absorbance of blank (570 nm) - Absorbance of blank (650 nm)]

Viable cell rate = (average measurement value of test substance / average measurement value of negative control) x 100 (average value is the average of n = 3 tests)

I-3. Judgment Criteria: Viable cell rate: 50% or less... Determined to be irritating.
Viable cell rate higher than 50%: Determined as non-irritating.

Judgment: The viable cell rate was 104%, and no skin irritation was observed in the surfactant preparation prepared this time.

In Japan, solutions of didecyldimethylammonium chloride (DDAC) with a concentration of 0.4 wt% or more are designated as a deleterious substance. However, the anti-enveloped virus cleaning agent of the present invention exhibits anti-influenza activity in a 0.004 wt% aqueous solution with a concentration of 1/100 of 0.4 wt%, which is the lower limit of DDAC's designation as a deleterious substance, and does not cause any damage to the skin. (Cell survival rate: 104%) was confirmed.

(Manufacturing example)
An anti-enveloped virus detergent (DEA-171) was produced as follows.
(1) Production of 100-fold concentrated solution of DEA-171 (antiviral cleaning composition) Biocide ST-70H (manufactured by Taisho Technos): Dialkyldimethylammonium cation (1.4g, titer as didecyldimethylammonium chloride: 0. 8g), sulfobetaine (20g, Amhitol 20HD: Contains 30% lauryldimethyl-2-hydroxypropyl-3-sulfobetaine), Neugen TDX80D (Daiichi Pharmaceutical Co., Ltd.): Poly(oxyethylene) alkyl ether (4. 0g) was mixed with water (150g, Lawson natural water (hardness 59)) and made homogeneous (pH = 7.6). To this, 20 g (0.4 g as EDTA) of a pH 7.0 aqueous solution of 2% EDTA (2Na ethylenediaminetetraacetic acid) dissolved in "Lawson Tennensui" prepared in advance was slowly added. Once it became homogeneous, the total amount was made up to 200 g with water to obtain an antiviral cleaning composition (100 times concentrated solution of DEA-171). The pH of this cleaning composition was 7.1.
(2) Production of DEA-171 and DEA-172 (4-fold diluted solution of DEA-171) 20 g of the antiviral cleaning composition (100-fold concentrated solution of DEA-171) produced in (1) was added to water (Tokyo 2 kg of DEA-171 was produced by diluting it with 1.98 kg of tap water (hardness 61) from Katsushika Ward, Miyako. pH was 6-8.

The composition of DEA-171 is as follows.
Part by mass Dialkyldimethylammonium salt 0.007
(0.004 as didecyldimethylammonium chloride)
Sulfobetaine 0.030
Poly(oxyethylene) = alkyl ether 0.020
EDTA 0.002
Wednesday 99.941
Total 100

Furthermore, 300 g of water (tap water in Katsushika Ward) was added to 100 g of this DEA-171 to prepare DEA-172 (anti-envelope virus cleaning agent). pH was 6-8.

(Example 11) New coronavirus inactivation test 900 μL of anti-envelope virus detergent (DEA-171) was added to 100 μL of virus solution, and after reacting for 1 minute and 30 minutes, the reaction solution was diluted stepwise by 10 times. Immediately, the virus titer of the reaction solution was measured by the plaque method (all measurements were performed three times).
Regarding the effectiveness of inactivation ability obtained by the plaque method, ◎: Sufficient effect (3.8 Log10 or more), ○: Effective (2 Log10 or more and less than 3.8 Log10), ×: No effect (less than 2 Log10) And so.

In each strain, the virus was inactivated to below the detection limit within 1 minute of sensitization time.
Also, unlike alcohol, persistence for 30 minutes or more was observed.

Subsequently, DEA-172 was evaluated in the same manner.

This means that dialkyldimethylammonium cations are currently effective at a concentration of 0.01 wt% (contact time of 5 minutes), which is said to be effective against the new coronavirus, but it is effective at about one-tenth of the concentration and one-fifth of the time. It means that it has come out.

(Example 12) Biological safety (reverse mutation: Ames) test of DEA-171 The test method was based on OECD TG471.
Test and results:
A reverse mutation test was performed using five strains (TA100, TA98, TA1535, TA1537, WP2uvrA) described in OECD TG471. In this test, both S9Mix (-) and S9Mix (+) systems were tested using product concentrations, and as a result, no growth inhibition was observed in any of the strains, and reversion was more than twice that of the solvent control. No number of mutant colonies was observed. That is, the mutagenicity of DEA-171 is negative.

(Example 13) Biological safety (skin sensitization) test of DEA-171 A skin sensitization test was conducted as follows.
Test mouse; CBA/J (Charles River Japan)
Group 1; negative control (NC); acetone:olive oil (4:1 v/v)
Second group; DEA-171 (undiluted solution)
Third group; positive control (PC); 25% hexylcinnamaldehyde For each mouse, (1) body weight, (2) determination of erythema in the auricle, and (3) ATP concentration in the lymph nodes of the auricle were measured.

Test results:
(1) No abnormality in weight gain or loss was observed in mice coated with DEA-171. (2) No erythema was observed. (3) No change in ATP concentration was observed.
Therefore, no skin sensitization was confirmed with DEA-171.

(Example 14) Confirmation of antibacterial activity of DEA-171 and DEA-172 DEA-171 and DEA-172 exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus from 30 seconds to 10 minutes.

As described above, the anti-enveloped virus cleaning agent (antiviral cleaning agent) of the present invention can be composed of 99% or more of water, is neutral, tasteless, odorless, transparent, and does not cause skin sensitization or mutagenicity. The drug was negative in both tests, and no abnormalities were observed after a single oral administration, making it highly safe.

In the present invention, by combining components (A) and (B), we have reduced the concentration of quaternary ammonium salts, which are problematic for use on the human body when used alone, and have reduced the effect on the human body. Cleaning agents can be provided.
This is because component (B), which has not been reported to have an inactive effect on enveloped viruses when used alone, has an inactive effect on enveloped viruses when combined with a specific quaternary ammonium salt of component (A). This is based on the inventors' first discovery of an unexpected synergistic effect that improves the
In particular, component (B) can adjust the balance between hydrophobicity and hydrophilicity of sulfobetaine or adjust the distance between positive charges and negative charges by appropriately selecting the substituent. By appropriately adjusting these substituents depending on the component (A) to be combined, a desired synergistic effect (inactivation effect against enveloped viruses) can be exerted.

Furthermore, the anti-enveloped virus detergent of the present invention exhibits strong inhibitory activity not only against influenza viruses but also against the enveloped virus, the novel coronavirus, and its mutant strains. This suggests that the cations (positive charges) of components (A) and (B) attack the negatively charged (anions) phospholipids constituting the envelope, thereby inactivating the enveloped virus. In addition, even for viruses whose RNA genes are prone to mutation, such as the new coronavirus, the phospholipids in the envelope are derived from infected cells, so they are not affected by virus mutations and have strong inhibitory activity against various mutant strains of the new coronavirus. shows.

Previously, it was thought that the combination of a quaternary ammonium salt and a betaine-type zwitterionic surfactant could reduce the bactericidal activity of the quaternary ammonium salt due to the ionic interaction of the surfactants. . The present invention surprisingly overturns this conventional view and produces the unexpected and remarkable effects described above.

As described above, the anti-enveloped virus cleaning agent of the present invention has a clear mechanism of action, is neutral and highly safe, and is highly effective specifically for enveloped viruses (regardless of the virus gene type or mutation). It is a cleaning agent that deactivates viruses and can be provided at low cost. Therefore, it is highly useful as an effective infectious disease countermeasure against the serious risk of the rapid spread of infectious diseases caused by the new coronavirus, which the international community is facing.

Note that the present invention is not limited to the above embodiments. The above-mentioned embodiments are illustrative, and any embodiment that has substantially the same configuration as the technical idea stated in the claims of the present invention and has similar effects is the present invention. covered within the technical scope of.

Claims (7)

Contains the following components (A) and (B) in the following proportions to the entire composition, and also contains water as the component (C), and the pH of the composition (JIS Z 8802: 2011 "pH measurement method") ) is 6 to 8 at 25°C.
(A) Dialkyldimethylammonium salt represented by the following general formula (1): 0.0001 to 0.4% by mass,

(In the formula, R ₁ and R ₂ are linear, branched or cyclic C 8 to C18 alkyl groups, R ₃ and R ₄ are methyl groups , and X is a fluorine atom, a chlorine atom, a bromine atom, a halogen atom, a hydroxy group, an alkoxy group, an acyloxy group, an alkylsulfonate group, a dialkylphosphonate group, an adipate group, a methosulfate group, a bicarbonate group, or a carbonate group selected from atom and iodine atom).
(B) Sulfobetaine represented by the following general formula (2) as an amphoteric surfactant: 0.003 to 3.0% by mass

(In the formula, R ₅ is an optionally substituted benzyl group or an optionally branched C2 to C18 alkyl group. R 6 and _R 7 _are a methyl group. Y is a C1 to C3 alkylene group. (However, if Y is a C3 alkylene group, a substituent may be placed at the 2-position.) The anti-envelope virus cleaning agent according to claim 1 , wherein the Y is any one of a methylene group, an ethylene group, a trimethylene group, and a 2-hydroxypropyl group . The anti-envelope virus cleaning agent according to claim 1 or 2, wherein the sulfobetaine is lauryldimethyl-2-hydroxypropyl-3-sulfobetaine . Target viruses include influenza virus, new coronavirus (SARS-CoV-2) and its variants, avian influenza virus, SFTS virus (severe fever thrombocytopenia syndrome), Ebola virus, coronavirus (MERS, SARS), dengue fever, and Zika. The anti-enveloped virus cleaning agent according to any one of claims 1 to 3 , characterized in that it is against fever, swine fever virus (CSFV), and ASFvirus (African swine fever). The target virus is the new coronavirus (SARS-CoV-2) and its mutant strains, and the component (A) is contained in a proportion of 0.0001 or more and less than 0.01% by mass based on the entire composition. The anti-enveloped virus detergent according to claim 4 , characterized in that: A disinfectant composition comprising the anti-envelope virus cleaning agent according to any one of claims 1 to 5 . The anti-enveloped virus cleaning agent according to any one of claims 1 to 5 or the disinfectant composition according to claim 6 is brought into contact with enveloped viruses to inactivate the enveloped viruses. Characteristic methods for inactivating enveloped viruses (excluding methods for surgery, treatment, or diagnosis of humans) .