JP7362014B2 - Production method of Cinnamon mushrooms, antioxidants, medicines, foods, and skin external preparations - Google Patents

Description

The technical field of the present specification relates to a method for producing a mandarin mushroom, an antioxidant, a pharmaceutical, a food, and an external skin preparation.

The mantis mushroom is a basidiomycete used in the herbal medicine ``reishi''. Manga mushrooms have a wide range of physiological activities and are widely used as Chinese medicine. Bamboo shoots contain active ingredients such as polysaccharides such as β-glucan and triterpenes such as ganoderic acid. β-glucan is known to have anticancer effects, immunostimulatory effects, and the like. Furthermore, ganoderic acid has been reported to have many effects, such as anticancer effects, blood pressure lowering effects, and cholesterol lowering effects. In addition, the extract of C. chinensis is known to have medicinal effects such as moisturizing effect, cell growth promoting effect, whitening effect, and collagen synthesis promoting effect.

Currently, most of the stone mushrooms on the market are artificially cultivated. There are two main cultivation methods used: log cultivation and fungal bed cultivation. Various cultivation methods have been developed with the aim of increasing the yield and active ingredients of Cinnamon mushroom. For example, Patent Document 1 discloses a method for cultivating C. chinensis using chlorella hot water extraction residue and dry powder of Coccomyx aeruginosa as medium components (Patent Document 1).

Japanese Patent Application Publication No. 2014-158441 Unexamined Japanese Patent Publication No. 2012-54

By the way, in the cultivation of ordinary mushrooms, a technique has been developed for applying electrical stimulation to the mushrooms. For example, Patent Document 2 discloses a technique in which a cultivation body side electrode 1a is inserted into a hoda tree and a spark discharge is generated between the cultivation body side electrode 1a and a discharge electrode 20 (see FIG. 1 etc. of Patent Document 2). ). It is disclosed that this increases the yield of mushrooms (see paragraph [0024] of Patent Document 2).

However, unlike edible mushrooms, the active ingredients extracted from Cypress mushrooms are used for the aforementioned medicinal properties. Therefore, when producing Cinderella mushrooms, it is necessary to increase the yield as well as the amount of active ingredients. The active ingredient is, for example, ganoderic acid, which has anticancer effects, antihypertensive effects, and cholesterol-lowering effects. Furthermore, it is preferable that the antioxidant effect of the mushroom increases.

The technology described in this specification has been developed in order to solve the problems of the conventional technology described above. The object is to provide a method for producing C. chinensis, an antioxidant, a pharmaceutical, a food, and an external preparation for skin, which can increase the yield of C. chinensis and increase the efficacy or active ingredients.

A first aspect includes a first electrode body including a first conductor, a second electrode body having a second conductor facing the first conductor, and a first conductor and a second conductor. and a voltage applying unit that applies an alternating voltage between the plasma generator and the plasma generator. Plasma is irradiated to the culture log or the culture bed while the second conductor of the electrode body is in indirect or direct contact with the culture log or the culture bed, and the culture log or the culture bed is irradiated with plasma. This is a method for producing stone mushrooms characterized by containing water containing minerals.

With this method of producing stone mushrooms, the yield of stone mushrooms is higher than conventional methods. The extract obtained by this production method exhibits a higher antioxidant effect than the conventional extract of Stonecroceum. Moreover, the content of ganoderic acid contained in the Ganoderma mushroom obtained by this production method is higher than the content of ganoderic acid contained in the conventional Ganoderma mushroom. Thus, this method of producing mountain mushrooms increases yield and at the same time increases potency or active ingredients.

The present specification provides a method for producing Stone mushrooms, antioxidants, pharmaceuticals, foods, and skin preparations that can increase the yield of Stone mushrooms and increase the efficacy or active ingredients.

It is a schematic block diagram showing the structure of the 1st plasma generation device of a 1st embodiment. It is a schematic block diagram showing the structure of the 2nd plasma generation device of a 1st embodiment. FIG. 3 is a diagram illustrating a state in which a bacterial bed is irradiated with plasma using the first plasma generator in the first embodiment. FIG. 2 is a diagram (part 1) showing how a fungal bed is irradiated with plasma using the second plasma generator in the first embodiment. FIG. 2 is a diagram (part 2) showing how a fungal bed is irradiated with plasma using the second plasma generator in the first embodiment. It is a graph showing an HPLC chromatogram of Reishi mushroom extract.

Hereinafter, specific embodiments will be described with reference to the drawings, taking as examples a method for producing a mandarin mushroom, an antioxidant, a pharmaceutical, a food, and an external preparation for the skin. The extract of the fruiting body of C. chinensis contains one or more components. In the embodiments and examples, the components of the C. chinensis extract may be simply referred to as components.

(First embodiment)
A first embodiment will be described. The first embodiment is a method for producing stone mushrooms. The method for producing stone mushrooms according to the present embodiment is a method for producing stone mushrooms using a plasma generator.

1. Ganoderma The strain of Ganoderma according to the present embodiment is a basidiomycete used in the crude drug "Reishi", and belongs to the family Ganodermataceae and the genus Ganoderma. Mushrooms of the genus Ganoderma include red reishi (reishi), black reishi (black reishi), purple reishi (purple mushroom), and green reishi in the ancient Chinese pharmacological texts "Bencao Gangme" and "Shennong Bencao Jing". (Blue grass), Huang Lingzhi (Yellow grass), and White Lingzhi (White grass) are described as being present. Kazuno reishi is also mentioned as a type of red reishi. The scientific name of Red Reishi and Kazuno Reishi is (Ganoderma lucidum). The scientific names of black reishi are (G. atrum, G. japonicum, G. sinense).

As the strain of Cinnamon mushroom, those distributed in the Chinese and Japanese markets can be used. Alternatively, it may be produced by separating and culturing from the tissues or the like of C. chinensis.

2. Plasma generator 2-1. First plasma generator 2-1-1. Structure of the first plasma generator FIG. 1 is a schematic configuration diagram showing the structure of the first plasma generator 100 of this embodiment. The first plasma generator 100 generates atmospheric pressure plasma by dielectric barrier discharge. The first plasma generating device 100 generates plasma between the object O1 and the object O1 placed between the two electrode bodies. It is preferable that the object O1 is a conductor. Alternatively, it is preferable that the material has some degree of conductivity. The object O1 may have a thin insulating layer such as a plastic bag on the outside.

As shown in FIG. 1, the first plasma generator 100 includes a first electrode body 110, a second electrode body 120, and a voltage application section 130. The first electrode body 110 has a first electrode 111 and a dielectric 112. The first electrode 111 is a first conductor. The first electrode 111 is made of metal, for example. Examples of the material for the first electrode 111 include copper and aluminum. Of course, other metals or alloys may be used. Further, the first electrode 111 is a flat plate electrode.

The dielectric 112 has a flat plate shape. The dielectric 112 is in contact with the first electrode 111 . The dielectric 112 is provided on the surface 111a of the first electrode 111 facing the second electrode 121. That is, the dielectric 112 covers the surface of the first electrode 111. Dielectric 112 has a surface 112a. Examples of the dielectric include quartz glass and ceramics. Of course, other dielectrics may be used.

The second electrode body 120 has a second electrode 121. The second electrode 121 is a second conductor. The second electrode 121 is made of metal, for example. Examples of the material of the second electrode 121 include copper and aluminum. Of course, other metals or alloys may be used. The second electrode 121 is a flat plate electrode. The second electrode 121 has a surface 121a facing the first electrode body 110.

Further, the area of the flat plate of the dielectric 112 is larger than the area of the flat plate of the first electrode 111. Therefore, when viewed from the second electrode 121, the surface 111a of the first electrode 111 is hidden behind the dielectric 112.

The first electrode body 110 and the second electrode body 120 are opposing electrodes that face each other. A surface 112a of the dielectric 112 of the first electrode body 110 and a surface 121a of the second electrode 121 of the second electrode body 120 are opposed to each other. Furthermore, the surface 111a of the first electrode 111 of the first electrode body 110 and the surface 121a of the second electrode 121 of the second electrode body 120 are opposed to each other.

2-1-2. Plasma Emission Area of the First Plasma Generator As shown in FIG. 1, the voltage application section 130 applies an AC voltage between the first electrode 111 and the second electrode 121. Here, the AC voltage is, for example, 1 kV or more and 10 kV or less. The frequency is 5 kHz or more and 100 kHz or less. These are just examples, and other values may be used. Since the object O1 is a conductor, the second electrode 121 and the object O1 have almost the same potential. On the other hand, a dielectric 112 is arranged between the first electrode 111 and the object O1. That is, the first electrode 111 and the object O1 are insulated. A strong electric field is then formed in the space between the first electrode 111 and the object O1. When the voltage between the first electrode 111 and the object O1 caused by this electric field exceeds the discharge starting voltage, a discharge occurs between the dielectric 112 and the object O1. Then, plasma is generated in the plasma emission region P1. The plasma emission region P1 is an area that emits light due to the generation of plasma.

That is, as shown in FIG. 1, the first plasma generation device 100 generates a plasma emission region P1 at a position between the dielectric 112 and the object O1. The first electrode 111 and the second electrode 121 are flat plate electrodes. Then, due to the voltage applied by the voltage application unit 130, discharge occurs at various locations in the region where the dielectric 112 and the object O1 face each other. Therefore, the plasma emission region P1 has a planar shape corresponding to the flat plate shape of the dielectric 112. Plasma in the plasma emission region P1 is generated almost uniformly on the upper surface O1a of the object O1. Furthermore, the plasma emission region P1 is located inside a space sandwiched between the first electrode body 110 and the second electrode body 120.

2-2. Second plasma generator 2-2-1. Structure of the second plasma generator FIG. 2 is a schematic configuration diagram showing the structure of the second plasma generator 200 of this embodiment. The second plasma generator 200 generates atmospheric pressure plasma by dielectric barrier discharge. The second plasma generator 200 irradiates the object O1 with plasma generated by discharging between two electrode bodies. When using the second plasma generator 200, the object O1 is not required to have electrical conductivity.

As shown in FIG. 2, the second plasma generator 200 includes a housing 201, a first electrode body 210, a second electrode body 220, a voltage application section 230, a gas supply section 240, and an irradiation port 201a. and has.

The first electrode body 210 includes a first electrode 211 and a dielectric 212 . The first electrode 211 is a conductor. The first electrode 211 is made of metal, for example. The first electrode 211 has a cylindrical shape. Further, the dielectric body 212 also has a cylindrical shape. The dielectric 212 is placed inside the first electrode 211 .

The second electrode body 220 has a second electrode 221 . The second electrode 221 is a rod-shaped electrode. Furthermore, the dielectric 212 is arranged at a position between the first electrode 211 and the second electrode 221.

The gas supply unit 240 supplies gas to the inside of the casing 201. Therefore, the gas supplied by the gas supply unit 240 is supplied between the first electrode body 210 and the second electrode body 220. The gas supplied by the gas supply section 240 is, for example, a rare gas such as Ar or He. Alternatively, it may be the atmosphere. When the gas supply unit 240 supplies a rare gas such as Ar, the second plasma generation device 200 easily generates plasma.

2-2-2. Plasma Emission Area of Second Plasma Generator As shown in FIG. 2, the voltage application section 230 applies an AC voltage between the first electrode 211 and the second electrode 221. A strong electric field is formed in the space between the first electrode 211 and the second electrode 221. When the voltage between the first electrode 211 and the second electrode 221 caused by this electric field exceeds the discharge starting voltage, a discharge occurs between the dielectric 212 and the second electrode 221. Then, plasma is generated in the discharge region.

Since the gas supply unit 240 supplies gas to the inside of the casing 201 of the second plasma generator 200, plasma is irradiated from the irradiation port 201a. Then, as shown in FIG. 2, a plasma emission region P2 is generated outside the irradiation port 201a. The plasma light emitting region P2 is a region that emits light due to the generation of plasma.

3. Method for irradiating raw wood or fungal bed with plasma 3-1. First Plasma Generator FIG. 3 is a diagram showing a state in which a bacterial bed is irradiated with plasma using the first plasma generator 100 in this embodiment. The fungal bed B1 has an upper surface B1a. The upper surface B1a has an uneven shape. In FIG. 3, the uneven shape of the upper surface B1a is drawn in a larger size for the sake of explanation. Although FIG. 3 depicts the fungal bed B1, it can be used for logs as well. Here, the raw wood and the fungus bed contain water containing minerals. Therefore, logs and fungal beds are considered to have some degree of conductivity.

As shown in FIG. 3, the second electrode 121 of the second electrode body 120 is in contact with the lower surface B1b of the bacterial bed B1. The dielectric 112 of the first electrode body 110 is in contact with the upper surface B1a of the fungal bed B1. The first electrode 111 of the first electrode body 110 is not in contact with the upper surface B1a of the bacterial bed B1.

As shown in FIG. 3, there is a slight gap between the dielectric 112 of the first electrode body 110 and the upper surface B1a of the fungal bed B1. The gap becomes the plasma emission region P1m. The plasma emission region P1m is an area that emits light due to plasma generation.

As shown in FIG. 3, the plasma emission region P1m is in contact with the fungal bed B1.

In FIG. 3, when viewed from vertically above, the size of the dielectric 112 of the first electrode body 110 is smaller than the size of the upper surface B1a of the fungal bed B1. Therefore, the plasma emission region P1m is in contact with a part of the upper surface B1a of the fungal bed B1. The upper surface B1a of the fungal bed B1 includes a region irradiated with plasma and a region not irradiated with plasma. In other words, a part of the upper surface B1a of the bacterial bed B1 is irradiated with atmospheric pressure plasma.

3-2. Second Plasma Generator FIG. 4 is a diagram (part 1) showing how a bacterial bed is irradiated with plasma using the second plasma generator 200 in this embodiment. As shown in FIG. 4, the plasma emission region P2 is in contact with the upper surface B1a of the bacterial bed B1. Therefore, ions, radicals, etc. contained within the plasma emission region P2 are irradiated onto the fungal bed B1.

FIG. 5 is a diagram (Part 2) showing how a bacterial bed is irradiated with plasma using the second plasma generator 200 in this embodiment. As shown in FIG. 5, the plasma emission region P2 is not in contact with the upper surface B1a of the bacterial bed B1. Therefore, ions, radicals, etc. contained in the area outside the plasma emission area P2 are irradiated onto the bacterial bed B1.

In this way, the plasma emission region P2 can be brought into contact with the bacterial bed B1. Moreover, it is also possible to prevent the plasma emission region P2 from coming into contact with the fungal bed B1. Further, the gas supplied by the gas supply unit 240 can be selected. The gas is preferably Ar. This is because plasma can be suitably generated.

3-3. Differences between the first plasma generator and the second plasma generator The first plasma generator 100 can generate planar plasma. Therefore, the raw wood or the fungal bed can be efficiently irradiated with plasma. The plasma generated by the first plasma generator 100 is non-equilibrium atmospheric pressure plasma. Further, the plasma gas is the atmosphere around the first plasma generator 100. Therefore, relatively many molecules, ions, and radicals derived from nitrogen atoms and oxygen atoms are generated in the plasma.

The second plasma generator 200 can irradiate plasma using the gas supplied by the gas supply section 240. For example, if Ar gas is used, plasma can be easily generated. In this case, it is considered that there are many Ar atoms in an excited state inside the plasma emission region P2. In the area outside the plasma emission region P2, the surrounding atmosphere is involved in the plasma, and it is considered that a relatively large number of molecules, ions, and radicals derived from nitrogen atoms or oxygen atoms originating from the surrounding atmosphere are generated.

3-4. Plasma irradiation conditions The time for continuous irradiation of logs or fungal beds each time is, for example, 30 seconds or more and 5 minutes or less. Preferably, the time is 1 minute or more and 2 minutes or less. This is because if the plasma irradiation time per time is long, the surface temperature of the raw wood or the fungus bed may rise. The surface temperature of the log or fungal bed is preferably 200°C or lower. For this purpose, it is advisable to provide appropriate intervals. When the plasma irradiation time per time is 1 minute, the number of plasma irradiations is preferably 3 or more and 5 or less. The above is an example, and conditions other than the above may be used.

As described above, when the first plasma generator 100 is used, a portion of the upper surface B1a of the bacterial bed B1 is irradiated with atmospheric pressure plasma. The irradiation area is preferably as follows. The ratio of the area of the dielectric 112 to the total area of the upper surface B1a of the fungal bed B1 is, for example, 5% or more and 50% or less. Here, the total area of the upper surface B1a of the fungal bed B1 is not the surface area of the upper surface B1a, but the area of the upper surface B1a when viewed from vertically above. This area ratio may be in a numerical range other than the above. Preferably, it is 5% or more and 35% or less. More preferably, it is 5% or more and 20% or less.

4. Method for producing Stone Mountain mushrooms The method for producing Stone Mountain mushrooms according to the present embodiment includes an inoculation step of inoculating logs or fungal beds with a strain of Stone Mountain mushrooms, a culturing step of culturing the logs or fungal beds into cultured logs or cultured fungus beds, and a cultivation step of cultivating the logs or fungus beds into cultured logs or cultured fungus beds. The method includes a plasma irradiation step of irradiating a cultured fungus bed with plasma, and a cultivation step of cultivating a cultured log or a cultured fungus bed to obtain a fruiting body of a mantis mushroom.

4-1. Material Preparation Step The logs used for cultivating stone mushrooms are preferably broad-leaved trees or coniferous trees. Examples of broad-leaved trees include sawtooth oak, Quercus serrata, and beech. Examples of coniferous trees include pine, fir, and hemlock. The above-mentioned tree can be cut using a round cutter or the like, placed in a plastic bag or the like, and heat sterilized by a known method. The sterilization method may be selected as appropriate.

The fungal bed used for cultivating the mushroom is a medium that appropriately contains carbon sources, nitrogen sources, inorganic substances, and other necessary nutrients that can be assimilated by the fungal strain. Examples of the fungal bed include a mixture of sawdust (for example, made from broad-leaved trees such as sawtooth oak, Quercus oak, and beech, or from coniferous trees such as pine, fir, and hemlock), rice bran, bran, corncob meal, water, etc. It will be done. Then, these culture media can be placed in a commercially available plastic bag for bacterial bed cultivation, and heat sterilized by a known method. The sterilization method may be selected as appropriate.

4-2. Inoculation process: Inoculate the mushroom strain onto logs or fungal beds. A known method may be used for inoculation.

4-3. Cultivation process: The mushroom strains inoculated on logs or fungus beds are allowed to take hold and propagate. This culturing step is preferably carried out in the dark. Of course, it's not limited to dark places. The culture temperature is, for example, 15°C or higher and 35°C or lower. Preferably, the temperature is 20°C or higher and 30°C or lower. The culture humidity is, for example, 40% or more and 80% or less. Preferably, it is 55% or more and 65% or less. The culture period in the log is preferably, for example, 40 days or more and 90 days or less. The culture period in the fungal bed is preferably, for example, 30 days or more and 80 days or less. Of course, the culture conditions are not limited to those described above.

The cultivation process is often completed when the entire log or fungus bed is uniformly covered with white mycelia. At this stage, hyphae derived from the fungal strain are prevalent in the log or fungus bed. Logs that have been cultured and are infested with mycelia are called "cultured logs." In addition, a fungal bed in which hyphae have spread after cultivation is completed is called a "cultured fungal bed."

4-4. Plasma irradiation process The plasma irradiation process is carried out on the cultured logs or cultured fungi bed after the culture process has been completed. For example, plasma is irradiated three times or more and five times or less. For example, the irradiation time per time is 1 minute.

4-5. Cultivation process Cultivate cultured logs or cultured fungus beds that have been irradiated with plasma and grow them to fruiting bodies. The fruiting bodies of Cinnamon mushroom grow primarily from the top surface of cultured logs or cultured fungi beds. This upper surface includes a region that has been irradiated with plasma and a region that has not been irradiated with plasma. The effect of plasma irradiation also extends to unirradiated regions that are not irradiated with plasma. The cultivation temperature is, for example, 20°C or higher and 35°C or lower. Preferably, the temperature is 25°C or higher and 30°C or lower. The cultivation humidity is, for example, 70% or more. Preferably it is 90% or more. By cultivating it in this way, a mandarin mushroom can be obtained.

5. Differences from conventional technology In the conventional technology, a large current is passed through the wood, etc. It is thought that this causes some kind of effect and causes the fungi to grow actively.

In this embodiment, a culture log or a culture bed is irradiated with non-equilibrium atmospheric pressure plasma. Therefore, charged particles may reach the culture log or culture bed. Therefore, a very weak electric current may flow through the culture log or culture bed. However, unlike the conventional method, the purpose of this method is not to pass a high current through a tree or the like.

Furthermore, by irradiating the plasma, ions and radicals derived from nitrogen atoms and oxygen atoms are irradiated onto the culture log or culture bed. It is expected that changes will occur in the components of the mandarin mushroom due to stimulation from such ions and radicals. For example, it is expected that the content of antioxidants in Cinnamon mushrooms will increase when stimulated by radicals with strong oxidizing effects.

6. Effects of this embodiment 6-1. Harvest Amount The method for producing stone mushrooms according to the present embodiment increases the harvest amount of stone mushrooms. It is thought that the irradiation with plasma activates the strain of C. chinensis. Note that a dielectric insulates the first electrode and the second electrode. Further, the object O1 is mainly placed in a plastic bag or the like. Therefore, the log or the fungus bed and the second electrode are insulated. Therefore, it is thought that the current does not flow through the log or the fungus bed, or even if it does flow through the log or the fungus bed, the amount of current is very small.

6-2. Antioxidant Effect The method for producing C. chinensis according to the present embodiment enhances the antioxidant effect of C. chinensis. For example, it is thought that the concentration of antioxidants in Cinnamon mushrooms is increased.

6-3. Ganoderic acid The method for producing Ganoderic mushrooms of the present embodiment increases ganoderic acid in Ganoderic mushrooms. Ganoderic acid has anticancer effects, blood pressure lowering effects, cholesterol lowering effects, and the like.

6-4. Plasma In this embodiment, a culture log or culture bed is irradiated with non-equilibrium atmospheric pressure plasma. As a result, it is thought that a small amount of active oxygen species is irradiated to the hyphae and the like, activating the bacteria. In fact, it is observed that the hyphae are once fragmented, and when the hyphae regenerate, they spread out in a network.

7. Modification 7-1. First Electrode of First Plasma Generator The first electrode 111 may have a curved surface on the side facing the second electrode 121. That is, the first electrode 111 may have a concave surface or a convex surface relative to the second electrode 121. Further, the first electrode 111 may have a curved surface. The first electrode 111 may have an uneven shape in addition to the above.

The dielectric 112 of the first electrode body 110 may also have a curved or bent surface corresponding to the shape of the first electrode 111. Further, the first plasma generating device may include the first electrode 111 having a flat plate shape and the dielectric body 112 having an uneven shape. In this way, the dielectric 112 may have a shape other than the shape corresponding to the first electrode 111.

7-2. Second Electrode of First Plasma Generator Instead of placing the plate-shaped second electrode 121 under the object O1, another second electrode may be used. For example, a rod-shaped second electrode may be inserted into the log or the fungus bed. In this case, the second electrode is in electrical continuity with the log or the fungal bed. Alternatively, the belt-shaped second electrode may be wrapped around the raw wood or the fungus bed together with the plastic bag.

7-3. Plasma irradiation conditions The number of plasma irradiations and the continuous plasma irradiation time may of course be changed as appropriate.

7-4. Combination The above modifications may be freely combined.

8. Summary of the Present Embodiment In the method for producing Cinderella edulis according to the present embodiment, plasma is irradiated onto a culture log or a cultured bed of Physcomitrella edulis. Therefore, it is possible to increase the yield of stone mushrooms. Moreover, the content of ganoderic acid in the ganoderic acid can be increased.

(Second embodiment)
A second embodiment will be described. The second embodiment is a method for extracting components from Cinnamon mushroom.

1. BACKGROUND OF THE INVENTION The fruiting bodies of Mushroom mushrooms or their extracts are used, for example, in pharmaceuticals, quasi-drugs, cosmetics (external skin preparations), foods, and the like. In addition, the fruiting body or extract thereof also functions as an antioxidant.

The fruiting body of the mandarin mushroom itself can be chopped or crushed and used for making tea, furikake, etc.

The extract of C. chinensis can be used in pharmaceuticals, quasi-drugs, cosmetics, and foods. The dosage forms include, for example, lotions, creams, massage creams, milky lotions, gels, aerosols, essences, packs, cleaning agents, bath agents, foundations, powders, lipsticks, ointments, poultices, pastes, and plasters. , powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (including tinctures, liquid extracts, spirits, suspensions, lemonades, etc.), tablets, syrups , beverages, etc.

In the case of external use, the content of the extract of Mandarin mushroom is preferably 0.0001% by weight or more based on the total amount in terms of solid matter. Preferably, it is 0.001% by weight or more and 10% by weight or less. More preferably, it is 0.01% by weight or more and 5% by weight or less. If the amount of the extract of C. chinensis is less than 0.0001% by weight, sufficient effects cannot be expected. When the blending amount of the C. chinensis extract exceeds 10% by weight, the effect is not significantly enhanced.

For internal use, the intake amount varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc. The daily intake per adult is preferably 5 mg or more. Preferably, it is 10 mg or more and 5 g or less. More preferably, the amount is 100 mg or more and 1 g or less.

2. Method for Extracting Stone Bamboo Shoots The method for extracting Stone Mountain mushrooms of this embodiment is mainly heating extraction or normal temperature extraction. Alternatively, other known extraction methods may be used.

2-1. Heating Extraction A solvent (extraction solvent) is added to the dried material of Cinnamon mushroom to form a mixture, and the components of Cinnamon mushroom can be extracted while heating the mixture to obtain an extract.

2-2. Room Temperature Extraction A solvent (extraction solvent) is added to the dry material of Stone Mountain to make a mixture, and the components of Stone Mountain are extracted from the mixture at room temperature to obtain an extract.

Examples of extraction solvents include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol, glycerin, etc.). ), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.) Can be mentioned. Preferred are polar solvents such as water, lower alcohols, and liquid polyhydric alcohols. Particularly preferred are water, ethanol, 1,3-butylene glycol and propylene glycol. These solvents may be used alone or in combination of two or more.

3. Modification 3-1. Processing The extract of C. chinensis may be used as it is as an extracted solution. Further, the extracted solution may be subjected to treatments such as concentration, dilution, filtration, decolorization, deodorization, and drying, as necessary. Furthermore, the active ingredients may be concentrated or isolated by subjecting ganoderic acids and the like to column purification such as HPLC.

3-2. Additives Additives may be added to the C. chinensis extract to the extent that they do not impair the effects of the extract. Examples of additives include oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, ultraviolet absorbers, Examples include thickeners, pigments, antioxidants, whitening agents, chelating agents, lactose, diluents such as microcrystalline cellulose, lubricants, disintegrants, and the like.

3-3. Combination The above modifications may be freely combined.

In the examples, "%" indicates weight %. "Part" indicates part by weight.

1. Cultivation method 1-1. Log cultivation The type of log is broad-leaved tree (Quercus genus). Logs cut into pieces with a diameter of 5 to 25 cm and a height of about 13 cm were used. Absorbent paper was placed on the bottom of a polypropylene bag, and 3 kg of cut logs and 120 mL of water for moisturizing were placed in the bag. The bag was gently closed, and the logs in the bag were heat sterilized with steam and allowed to cool to room temperature in a clean space.

Next, the log was inoculated with 65 mL of a commercially available Reishi mushroom inoculum (product name: Mannentake, manufactured by Akiyama Seed Research Institute). After vaccination, the bag was closed. Thereafter, the cells were cultured in the dark at a temperature of 24 to 30°C and a humidity of 40 to 75%. The log was cultured until the entire log was covered in white with mycelia to obtain a cultured log. The culture period was 40-50 days.

The cultured logs were irradiated with atmospheric pressure plasma. The plasma irradiation conditions (AG) will be described later. After plasma irradiation, cultivation was performed at a temperature of 25 to 30°C, humidity of 90 to 100%, and illuminance of 3000 lux or less. After cultivation for 50 to 120 days, the fruiting bodies of C. chinensis were obtained.

1-2. Fungal bed cultivation The type of log used for the culture medium is broad-leaved trees (Quercus genus). The raw wood was ground into pieces with a size of 1 to 15 mm using a grinder. A culture medium was prepared by mixing 0.9 kg of crushed raw wood, 0.1 kg of wheat bran, and 1.5 kg of water, and the mixture was placed in a polypropylene bag to form a bacterial bed. The bag was gently closed, and the culture medium was heat sterilized with steam and allowed to cool to room temperature in a clean space.

Next, 54 mL of commercially available Reishi mushroom inoculum (product name: Mannentake, manufactured by Akiyama Seed Research Institute) was inoculated into the medium. After vaccination, the bag was closed. Thereafter, the cells were cultured in the dark at a temperature of 15 to 30°C and a humidity of 45 to 75%. The cells were cultured until the entire medium was covered with white hyphae to obtain a culture bed. The culture period was 60-120 days.

The culture bed was irradiated with atmospheric pressure plasma. The plasma irradiation conditions (AF) will be described later. After plasma irradiation, cultivation was performed at a temperature of 24 to 28°C, humidity of 75 to 85%, and illuminance of 3000 lux or less. After 30 days of cultivation, the fruiting bodies of C. monocytogenes were obtained.

2. Plasma irradiation conditions Tables 1 to 7 show the irradiation conditions of atmospheric pressure plasma to irradiate the culture logs or cultured fungi bed. In the irradiation conditions AC, the pen-shaped second plasma generator 200 was used, and in the irradiation conditions DG, the flat-type first plasma generator 100 was used. In addition, when using a flat type plasma generation device (first plasma generation device 100), the plasma irradiation area that occupies the entire area of the top surface of the culture log or culture bed is projected onto the top surface of the culture bed. The area ratio was about 7% to 11%. Note that the cultured logs and cultured fungi beds are cultured in a polypropylene bag. Therefore, plasma was irradiated with the cultured logs and cultured fungi bed in a bag. Therefore, under irradiation conditions E and G, plasma was irradiated in a state where polypropylene was present between the second electrode and the culture log or culture bed. In this case as well, a plasma emission region was generated between the first electrode and the culture log or the culture fungus bed.

[Table 1]
Irradiation conditions A
Plasma device pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L/min
Applied voltage 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 20mm (from the irradiation port 201a to the top surface B1a of the bacterial bed)
Light emitting area Non-contact with target

[Table 2]
Irradiation condition B
Plasma device pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L/min
Applied voltage 6.5kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 2 minutes (total irradiation time: 6 minutes)
Irradiation distance 10mm (from the irradiation port 201a to the top surface B1a of the bacterial bed)
Light emitting area Contact with target

[Table 3]
Irradiation conditions C
Plasma device pen type (second plasma generator)
Gas type Ar
Gas flow rate 3L/min
Applied voltage 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 10mm (from the irradiation port 201a to the top surface B1a of the bacterial bed)
Light emitting area Contact with target

[Table 4]
Irradiation conditions D
Plasma device Planar type (first plasma generator)
First electrode 50x50mm
Position of the first electrode: Near the edge of the top surface of the culture log or culture bed Second electrode φ1 110mm (insert the tungsten rod into the object)
Position of the second electrode: On the side surface at a height of 10 mm from the bottom surface (B1b)
Position closest to the first electrode body (100mm insertion)
Applied voltage 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 5]
Irradiation condition E
Plasma device Planar type (first plasma generator)
First electrode 50x50mm
Position of the first electrode: Near the edge of the top surface of the culture log or culture bed Second electrode: 250 x 200 mm (wider than the bottom area of the object)
Position of second electrode: Placed directly under the culture log or culture bed Applied voltage: 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 6]
Irradiation conditions F
Plasma device Planar type (first plasma generator)
First electrode 50x50mm
Position of the first electrode: Center of the top surface of the culture log or culture bed Second electrode φ1 110mm (insert the tungsten rod into the object)
Position of the second electrode: On the side surface at a height of 10 mm from the bottom surface (B1b)
Position closest to the first electrode body (100mm insertion)
Applied voltage 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

[Table 7]
Irradiation condition G
Plasma device Planar type (first plasma generator)
First electrode 50x50mm
Position of the first electrode: Near the edge of the top surface of the culture log or culture bed Second electrode: 50 x 750 mm
Position of the second electrode: Wrap it around the lower side of the culture log or culture bed Applied voltage: 5.2kV
Number of irradiations: 3 times (interval: 1 minute)
Irradiation time: 1 minute (total irradiation time: 3 minutes)
Irradiation distance 0mm
Light emitting area Contact with target

3. Harvest amount of Cinnamon mushroom Table 8 shows the yield of Cinnamon mushroom (adjusted to a moisture content of 10% by weight) obtained by log cultivation and fungal bed cultivation. In addition, in Cultivation Examples 1A-1G, the yields are shown when the yield of log cultivation without plasma irradiation is set as 100. In Cultivation Examples 2A to 2F, the yields are shown when the yield of fungal bed cultivation without plasma irradiation is set as 100.

[Table 8]
Sample Cultivation method Irradiation conditions Yield Cultivation example 1A Log cultivation Irradiation conditions A 109
Cultivation example 1B Log cultivation Irradiation conditions B 106
Cultivation example 1C Log cultivation Irradiation conditions C 97
Cultivation example 1D Log cultivation Irradiation conditions D 122
Cultivation example 1E Log cultivation Irradiation conditions E 154
Cultivation example 1F Log cultivation Irradiation conditions F 130
Cultivation example 1G Log cultivation Irradiation conditions G 130
Cultivation example 2A Fungal bed cultivation Irradiation conditions A 128
Cultivation example 2B Fungal bed cultivation Irradiation conditions B 121
Cultivation example 2C Fungal bed cultivation Irradiation conditions C 106
Cultivation example 2D Fungal bed cultivation Irradiation conditions D 106
Cultivation example 2E Fungal bed cultivation Irradiation conditions E 112
Cultivation example 2F Fungal bed cultivation Irradiation conditions F 101

As shown in Table 8, in Cultivation Examples 1D-1G and Cultivation Examples 2A-2B, the yield increased by 20% or more compared to the conventional case where plasma was not used. For log cultivation, it is preferable to use the flat type first plasma generator 100. For fungal bed cultivation, it is preferable to use a pen-shaped second plasma generator 200.

When using the pen-shaped second plasma generator 200, it is preferable to irradiate the culture log or culture bed with atmospheric pressure plasma while the light emitting region P2 is not in contact with the culture log or culture bed (cultivation). Examples 1A-1C, 2A-2C).

When using the planar first plasma generator 100, it is preferable to place the planar second electrode under the culture log or the culture fungus bed (Cultivation Examples 1E and 2E). In particular, the increase in yield was remarkable in log cultivation (Cultivation Example 1E).

4. Extract of Cinnamon mushroom The method for extracting components from Cinnamon mushroom will be explained below.

4-1. Extraction method a (hot water extract)
200 mL of purified water was added to 10 g of dry material of Cinnamon mushroom cultivated on logs or on fungi beds, and extracted at 95 to 100°C for 2 hours, filtered, and the filtrate was concentrated and freeze-dried to obtain a hot water extract. .

4-2. Extraction method b (50% ethanol extract)
200 mL of 50% ethanol was added to 10 g of dry material of Cinnamon mushroom grown on logs or in fungi beds, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain a 50% ethanol extract.

4-3. Extraction method c (ethanol extract)
200 mL of ethanol was added to 10 g of dry material of Cinnamon mushroom grown on logs or in fungi beds, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain an ethanol extract.

Table 9 shows the weight of the L. chinensis extract in each production example. In Table 9, "hot water" indicates that a hot water extraction method was used. "Water + ethanol" indicates that an extraction method using 50% ethanol was used. "Ethanol" indicates that an extraction method using ethanol was used.

Then, Cultivation Examples 1A-2F and Extraction Methods ac were combined to form Production Example 1Aa-2Fc. For example, "Manufacturing Example 1Aa" means that it was cultivated according to "Cultivation Example 1A" and extracted using "extraction method a".

5. Antioxidant effect of the extract of C. chinensis 5-1. Active Oxygen Species α,α-diphenyl-β-picrylhydrazyl (hereinafter referred to as “DPPH”), which is a type of free radical, was used as the active oxygen species.

5-2. Experimental method The extract corresponding to Production Example 1Aa-2Fc above was added to 0.4 mL of 0.1 M acetate buffer (pH 5.5) to a final concentration of 100 μg/mL, 0.4 mL of absolute ethanol, and 0.4 mL of absolute ethanol. 0.2 mL of 5 mM DPPH absolute ethanol solution was added to prepare a reaction solution. In addition, for the oil-soluble extract of Production Example 1Aa-2Fc, the sample was added to 0.4 mL of absolute ethanol to prepare a reaction solution. Thereafter, the reaction was carried out at 37° C. for 30 minutes, and the absorbance (J1) at a wavelength of 517 nm was measured using water as a control. Moreover, the absorbance (J2) was measured using purified water instead of the sample as a blank. The DPPH radical scavenging rate was calculated using the formula shown below.
DPPH radical scavenging rate (%) = (1-J1/J2) x 100

5-3. DPPH radical elimination rate Table 11 shows the DPPH radical elimination rate for each production example. As shown in Table 11, all of the extracts of C. chinensis have a DPPH radical scavenging effect. Extracts from C. chinensis , whose cultured logs or culture beds were irradiated with plasma, showed higher antioxidant activity than extracts from unirradiated C. chinensis . In particular, Production Examples 1Aa and 2Aa, in which plasma was irradiated using the pen-shaped second plasma generator 200 without bringing the light-emitting region into contact with the culture log or culture bed, showed strong antioxidant effects. In addition, Production Examples 2Da and 2Ea using the planar first plasma generator 100 also exhibited strong antioxidative effects.

[Table 11]
DPPH radical scavenging rate Production example 1Aa (log, irradiation condition A, hot water) 46
Production example 1Ca (log, irradiation condition C, hot water) 41
Comparative production example 1a (raw wood, unirradiated, hot water) 37
Production example 2Aa (bacteria bed/irradiation condition A/hot water) 45
Production example 2Ba (bacteria bed/irradiation condition B/hot water) 35
Production example 2Da (bacteria bed, irradiation conditions D, hot water) 41
Production example 2Ea (bacteria bed/irradiation condition E/hot water) 44
Production example 2Fa (bacteria bed/irradiation condition F/hot water) 35
Comparative production example 2a (bacteria bed, non-irradiated, hot water) 33

6. Ganoderic acid content Ganoderic acid has effects such as anticancer, blood pressure lowering, and cholesterol lowering effects.

6-1. Measurement method The content of ganoderic acids in the extract of Production Example 1Aa-2Fc was measured using HPLC. Table 12 shows the HPLC conditions. Furthermore, mobile phase K2 was changed linearly from 10% to 94% in 60 minutes. Further, the flow rate was adjusted so that the retention time of pyrene was about 52 minutes. For example, it is 1.0 mL/min.

[Table 12]
Column: Develosil ODS-HG-5 (manufactured by Nomura Chemical)
Column size: inner diameter 4.6mmφ x length 250mm
Column temperature: 40℃
Sample concentration: 1.0mg/mL
Injection volume: 10μL
Detection: PDA 254nm
Eluent (gradient):
Mobile phase K1 10mM sodium phosphate buffer (pH 6.9)
Mobile phase K2 Acetonitrile Internal standard solution: Pyrene ethanol solution (1 mg/mL)

6-2. Measurement Results of Ganoderic Acid Content FIG. 6 is a graph showing an HPLC chromatogram of Ganoderic acid extract. As shown in FIG. 6, many peaks derived from ganoderic acids having maximum absorption near 254 nm were confirmed within a retention time period of 15 to 25 minutes.

Table 13 shows the area values of peaks derived from ganoderic acids. Here, the area value in the case of non-irradiation was set as 1 as a reference. That is, for Production Example 1Ac-1Fc, Comparative Production Example 1c was used as the standard. Regarding Production Example 2Ac-2Fc, Comparative Production Example 2c was used as the standard. As is clear from Table 13, the content of ganoderic acid in the ganoderic acid increases by irradiating the culture logs or the culture bed with atmospheric pressure plasma. Production examples in which atmospheric pressure plasma was irradiated using the pen-shaped second plasma generator 200 contained a relatively large amount of ganoderic acid (Production Examples 1Ac and 2Ac).

Moreover, Production Example 2Ec contains a very large amount of ganoderic acid. The content of ganoderic acid in Production Example 2Ec is about 40% higher than that in the sample not irradiated with plasma. Furthermore, as described above, Production Example 2Ec exhibits high antioxidant activity.

[Table 13]
Ganoderic acid content Production example 1Ac (raw wood, irradiation conditions A, ethanol) 1.09
Production example 1Ec (log, irradiation condition E, ethanol) 1.01
Production example 1Gc (log, irradiation condition G, ethanol) 1.06
Comparative production example 1c (raw wood, unirradiated, ethanol) 1.00
Production example 2Ac (bacteria bed, irradiation conditions A, ethanol) 1.07
Production example 2Dc (bacteria bed, irradiation conditions D, ethanol) 1.05
Production example 2Ec (bacteria bed/irradiation condition E/ethanol) 1.39
Production example 2Fc (bacteria bed/irradiation condition F/ethanol) 1.05
Comparative production example 2c (bacteria bed, non-irradiated, ethanol) 1.00

7. Prescription Examples Prescription examples are shown in which extracts of C. chinensis were prepared according to Production Examples 1Aa to 2Fc. The mixed extract here refers to a mixture of equal amounts of extracts from each production example.

7-1. Formulation Example 1 Lotion Components 1 to 6 and 11 and components 7 to 10 in Table 14 are each uniformly dissolved, mixed and filtered. This yields a lotion as a product.

[Table 14]
Lotion Prescription Content (parts)
1. Production Example 1Aa, 1Da mixed extract 1.0
2.1,3-butylene glycol 8.0
3. Glycerin 2.0
4. Xanthan gum 0.02
5. Citric acid 0.01
6. Sodium citrate 0.1
7. Ethanol 5.0
8. Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance: Appropriate amount 11. Make the total volume 100 with purified water.

7-2. Formulation Example 2 Cream Ingredients 2 to 9 in Table 15 are heated and dissolved, mixed and kept at 70°C to form an oil phase. Components 1 and 11 to 13 are heated and dissolved, mixed, and kept at 75°C to form an aqueous phase. Add the water phase to the oil phase and emulsify. Thereafter, while stirring and cooling the emulsified solution, component 10 is added at 45°C, and further cooled to 30°C. This gives a cream as a product.

[Table 15]
Cream prescription content (parts)
1. Production Example 1 Mixed extract of Ab, 1Bb, 1Cb, 1Db, 1Eb 0.5
2. Squalane 5.5
3. Olive oil 3.0
4. Stearic acid 2.0
5. Beeswax 2.0
6. Octyldodecyl myristate 3.5
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8. Behenyl alcohol 1.5
9. Glyceryl monostearate 2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.25
12.1,3-butylene glycol 8.5
13. Make the total volume 100 with purified water.

7-3. Formulation Example 3 Cream The extract of Production Example 1Ab in Table 15 was replaced with the extract of Production Example 2Ab.

7-4. Formulation Example 4 Cream The extract of Production Example 1Ab in Table 15 was replaced with the extract of Production Example 2Bb.

7-5. Formulation Example 5 Cream The extract of Production Example 1Ab in Table 15 was replaced with the extract of Production Example 2Cb.

7-6. Formulation Example 6 Emulsion Components 2 to 8 in Table 16 are dissolved and mixed by heating, and kept at 70°C to form an oil phase. Components 1 and 10 to 13 are heated and dissolved, mixed, and kept at 75°C to form an aqueous phase. Add the water phase to the oil phase and emulsify. Thereafter, while stirring and cooling the emulsified solution, component 9 is added at 45°C, and the mixture is further cooled to 30°C. As a result, a milky lotion is obtained as a product.

[Table 16]
Emulsion prescription content (parts)
1. Production Example 1 Mixed extract of Ba, 1Ca, 1Ea, 1Fa, 1Ga 1.0
2. Squalane 5.0
3. Olive oil 5.0
4. Jojoba oil 5.0
5. Setanol 1.5
6. Glyceryl monostearate 2.0
7. Polyoxyethylene cetyl ether (20E.O.) 3.0
8. Polyoxyethylene sorbitan monooleate (20E.O.) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12. Methyl paraoxybenzoate 0.2
13. Make the total volume 100 with purified water.

7-7. Formulation Example 7 Gel Components 1 to 5 and components 6 to 11 in Table 17 are each uniformly dissolved and mixed. This yields a gel as a product.

[Table 17]
Gel formulation Content (parts)
1. Production Example 1 Mixed extract of Ac, 1Dc, 1Ec, 2Ac, 2Ec 0.01
2. Ethanol 5.0
3. Methyl paraoxybenzoate 0.1
4. Polyoxyethylene hydrogenated castor oil (60E.O.) 0.1
5. Fragrance Appropriate amount 6.1,3-butylene glycol 5.0
7. Glycerin 5.0
8. Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. Make the total volume 100 with purified water.

7-8. Formulation Example 8 Pack Components 1 to 11 in Table 18 are uniformly dissolved. As a result, a pack as a product is obtained.

[Table 18]
Pack Prescription Content (parts)
1. Production Example 2 Mixed extract of Aa and 2Fa 0.1
2. Production Example 2Da, 2Ea mixed extract 0.1
3. Polyvinyl alcohol 12.0
4. Ethanol 5.0
5.1,3-butylene glycol 8.0
6. Methyl paraoxybenzoate 0.2
7. Polyoxyethylene hydrogenated castor oil (20E.O.) 0.5
8. Citric acid 0.1
9. Sodium citrate 0.3
10. Fragrance: Appropriate amount 11. Make the total volume 100 with purified water.

7-9. Formulation Example 9 Foundation Components 2 to 8 in Table 19 are dissolved by heating and kept at 80°C to form an oil phase. Component 9 is sufficiently swollen in component 19, and then components 1 and 10 to 13 are added and mixed uniformly. Add components 14 to 17 that have been ground and mixed using a grinder, stir with a homomixer, and keep at 75°C to form an aqueous phase. Add the aqueous phase to the oil phase while stirring and emulsify. Then cool, add component 18 at 45°C, and cool to 30°C while stirring. As a result, a foundation as a product is obtained.

[Table 19]
Foundation prescription content (parts)
1. Production Example 1 Mixed extract of Fb, 1Gb, 2Db, 2Eb, 2Fb 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0
4. Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Setanol 1.0
6. liquid lanolin 2.0
7. Liquid paraffin 3.0
8. Isopropyl myristate 6.5
9. Carboxymethyl cellulose sodium 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12. Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14. Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Fragrance: Appropriate amount 19. Make the total volume 100 with purified water.

7-10. Formulation Example 10 Bath Agent Components 1 to 6 in Table 20 are mixed uniformly. As a result, a bath additive can be obtained as a product.

[Table 20]
Bath additive Prescription Content (parts)
1. Extract of Production Example 1Ea 5.0
2. Production Example 2 Aa extract 1.0
3. Sodium hydrogen carbonate 50.0
4. Yellow No. 202 (1) Appropriate amount 5. Fragrance (appropriate amount) 6. Make the total volume 100 with sodium sulfate.

7-11. Formulation Example 11 Ointment Components 2 to 5 in Table 21 are heated and dissolved, mixed and kept at 70°C to form an oil phase. Components 1 and 6 to 8 are heated and dissolved, mixed, and kept at 75°C to form an aqueous phase. Add the water phase to the oil phase to emulsify, and cool to 30°C while stirring. This yields an ointment as a product.

[Table 21]
Ointment Prescription Content (parts)
1. Production Example 1 Mixed extract of Ab, 1Eb, 2Ab, 2Eb 0.5
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glyceryl monostearate 10.0
4. Liquid paraffin 5.0
5. Setanol 6.0
6. Methyl paraoxybenzoate 0.1
7. Propylene glycol 10.0
8. Make the total volume 100 with purified water.

7-12. Formulation Example 12 Powder Mix ingredients 1 to 3 in Table 22. This gives a powder as a product.

[Table 22]
Powder Prescription Content (parts)
1. Production Example 1 Mixed extract of Aa, 1Ea, 2Aa, 2Ea 20.0
2. Dried cornstarch 30.0
3. Microcrystalline cellulose 50.0

7-13. Formulation Example 13 Tablet Components 1 to 4 in Table 23 are mixed, and then an aqueous solution of component 5 is added as a binder to form granules. Add component 6 to the formed granules and tablet. One tablet weighs 0.52 g. This yields tablets as a product.

[Table 23]
Tablet Prescription Content (parts)
1. Production Example 1 Mixed extract of Aa, 1Ea, 2Aa, 2Ea 3.0
2. Dried cornstarch 27.0
3. Carboxymethyl cellulose calcium 20.0
4. Microcrystalline cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6. Talc 3.0

7-14. Formulation Example 14 Tablet Confectionery Components 1 to 4 and 7 in Table 24 are mixed and formed into granules. Components 5 and 6 are added to the formed granules and compressed into tablets. One grain is 1.0g. As a result, a tablet confectionery product is obtained.

[Table 24]
Tablet confectionery prescription content (parts)
1. Production Example 1 Mixed extract of Aa, 1Ea, 2Aa, 2Ea 0.5
2. Dried cornstarch 50.0
3. Erythritol 40.0
4. Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6. Fragrance (appropriate amount) 7. Make the total volume 100 with purified water.

7-15. Formulation Example 15 Beverage Mix ingredients 1 to 5 in Table 25. As a result, a beverage as a product is obtained.

[Table 25]
Beverage Prescription Content (parts)
1. Production Example 1 Mixed extract of Aa, 1Ea, 2Aa, 2Ea 2.0
2. Fructose glucose liquid sugar 12.5
3. Citric acid 0.1
4. Fragrance 0.05
5. Make the total volume 100 with purified water.

7-16. Formulation Example 16 Powdered Beverage Mix ingredients 1 to 8 in Table 26. As a result, a powdered beverage is obtained as a product.

[Table 26]
Prescription example 16 Powdered drink Prescription Content (parts)
1. Production Example 1 Mixed extract of Aa, 1Ea, 2Aa, 2Ea 10.0
2. Powdered sugar 65.0
3. Powdered peach juice 15.0
4. L-ascorbic acid 8.0
5. Crystalline citric acid 1.2
6. Sodium citrate 0.75
7. Aspartame 0.02
8. Powdered peach flavor 0.03

A. Additional Notes In the method for producing stone mushrooms in the first aspect, a plasma generation device having a first electrode body including a first conductor is used. Plasma is irradiated onto the culture log or culture bed while the first conductor of the plasma generator is not in direct contact with the culture log or culture bed.

In the method for producing stone mushrooms in the second aspect, the first electrode body has a dielectric material. Plasma is irradiated onto the culture log or culture bed while the dielectric body is located between the first conductor and the culture log or culture bed.

In the method for producing stone mushrooms in the third aspect, the plasma generation device includes: a second electrode body having a second conductor; a voltage application unit that applies a voltage between the first conductor and the second conductor; has.

In the method for producing stone mushrooms according to the fourth aspect, the voltage application section is configured to connect the first conductor and the second conductor while the culture log or the culture fungus bed is placed on the second conductor of the second electrode body. A voltage is applied between it and the body.

In the method for producing stone mushrooms according to the fifth aspect, the plasma generating device generates a light emitting region. Plasma is irradiated onto the culture log or culture bed while the light-emitting region is not in contact with the culture log or culture bed.

The method for producing an antioxidant according to the sixth aspect includes the steps of the above-described method for producing Stone Mountain mushrooms, and an extraction step of extracting components from Stone Mountain mushrooms.

A method for producing a pharmaceutical product according to a seventh aspect includes the steps of the above-mentioned method for producing C. chinensis and an extraction step of extracting components from C. chinensis.

The food production method according to the eighth aspect includes the steps of the above-mentioned method for producing Stone Mountain mushroom, and an extraction step of extracting components from Stone Mountain mushroom.

The method for producing an external preparation for skin according to the ninth aspect includes the steps of the above-described method for producing C. chinensis and an extraction step of extracting components from C. chinensis.

100...First plasma generation device 200...Second plasma generation device 110, 210...First electrode body 111, 211...First electrode 112, 212...Dielectric body 120, 220...Second electrode body 121, 221...th 2 electrodes P1, P2, P1m...Plasma emission area B1...Bacteria bed B1a...Top surface B1b...Bottom surface

Claims (9)

A first electrode body including a first conductor, a second electrode body having a second conductor facing the first conductor, and an AC voltage applied between the first conductor and the second conductor. Using a plasma generation device having a voltage application unit that applies a voltage,
The second conductor of the second electrode body is brought into indirect contact with the culture log or the culture bed of the bamboo mushroom without directly contacting the first conductor of the plasma generating device with the culture log or the culture bed of the stone mushroom. irradiating the culture log or the culture fungus bed with plasma in a state of direct contact;
A method for producing stone mushrooms, characterized in that the culture log or the culture bed contains mineral-containing water. In the method for producing Cinderella mushrooms according to claim 1,
Cover the cultured logs or cultured beds of stone mushrooms with a layer of insulator,
irradiating the culture log or the culture fungus bed with plasma while the second conductor of the second electrode body is in indirect contact with the insulator layer from outside;
A method for producing stone mushrooms characterized by the following. In the method for producing Cinderella mushrooms according to claim 2,
The first conductor and the second conductor are flat electrodes,
arranging the culture log or the culture fungus bed on the second conductor,
The area of the surface of the second conductor including the contact surface with the insulator layer is
1. A method for producing a stone mushroom, characterized in that the area is larger than the area of the bottom surface of the culture log or the culture fungus bed. In the method for producing Cinderella mushrooms according to claim 2,
The second conductor is
A method for producing stone mushrooms, characterized in that the insulating layer is wrapped around the side surface of the layer. In the method for producing Cinderella mushrooms according to claim 1,
irradiating the culture log or the culture bed with plasma while the second conductor of the second electrode body is in direct contact with the culture log or the culture bed;
A method for producing stone mushrooms characterized by the following. The steps of the method for producing stone mushrooms according to any one of claims 1 to 5,
an extraction step of extracting components from the mandarin mushroom;
A method for producing an antioxidant, comprising: The steps of the method for producing stone mushrooms according to any one of claims 1 to 5,
an extraction step of extracting components from the mandarin mushroom;
A method for producing a pharmaceutical product characterized by having the following. The steps of the method for producing stone mushrooms according to any one of claims 1 to 5,
an extraction step of extracting components from the mandarin mushroom;
A method for producing a food product characterized by having the following. The steps of the method for producing stone mushrooms according to any one of claims 1 to 5,
an extraction step of extracting components from the mandarin mushroom;
A method for producing an external preparation for skin, characterized by having the following.

Images