JP7385243B2 - Composition for promoting amino acid absorption - Google Patents

Description

The present invention relates to a composition for promoting absorption of branched chain amino acids containing barley stems and/or leaves.

In recent years, against the background of increasing interest in health, various health foods have been developed, and various materials used in health foods are being researched.

For example, leucine, which is a type of branched chain amino acid, is known to play an important role in muscle synthesis, and is included in health foods such as supplements (see Patent Document 1). However, sufficient research has not been conducted on promoting absorption of branched chain amino acids.

International publication WO2013/021891

An object of the present invention is to provide a composition for promoting absorption of branched chain amino acids containing a substance exhibiting an excellent effect of promoting absorption of branched chain amino acids.

In order to solve the above-mentioned problem, the present applicant conducted intensive studies on various substances and surprisingly discovered that barley stems and/or leaves (hereinafter referred to as ``stems and/or leaves'') It has been found that a product that does not contain barley stems or leaves exhibits a much superior effect on promoting the absorption of branched chain amino acids. The present invention has been completed based on this knowledge.

The outline of the present invention is as follows.
<1> A composition for promoting absorption of branched chain amino acids, which contains barley stems and/or leaves.
<2> The composition for promoting absorption according to claim 1, wherein the branched chain amino acid is leucine.
<3> Oral composition containing barley stems and/or leaves and branched chain amino acids.
<4> The oral composition according to <3>, wherein the branched chain amino acid is leucine.

According to the present invention, branched chain amino acids can be efficiently absorbed into the body by containing barley stems and leaves.

The results of evaluation of leucine permeability using Caco-2 cells are shown.

The present invention will be explained in detail below.

[1. Barley stems and/or leaves]
The barley stems and leaves used in the present invention refer to stems and/or leaves obtained from barley (scientific name: Hordeum vulgare). Examples of barley that can be used include two-rowed barley, six-rowed barley, and naked barley, and these can be used alone or in combination of two or more. It is preferable that the stems and leaves of barley are those harvested before the ripening stage, that is, from the tillering start stage to before earing (barley young leaves).

The barley stems and leaves used in the present invention are preferably processed products, and specifically, crushed products and powder thereof (pulverized powder), squeezed juice and powder thereof (squeezed powder), extracts and powder thereof (extract powder), etc. In this specification, the term "powder" includes pulverized powder, squeezed powder, and extracted powder. The method for producing the crushed powder of barley stems and leaves used in the present invention is not particularly limited, but includes, for example, a method in which barley stems and leaves are cut, blanched, then dried, and then crushed. The method for producing juice from barley foliage is not particularly limited, but for example, barley foliage or its fragments are squeezed, centrifuged, or filtered, and the crude solids are removed. Here are some ways to get it. The squeezed juice may be concentrated as necessary, or it can be processed into a dry powder (squeezed powder) by freeze drying, hot air drying, spray drying, or the like. When obtaining an extract of barley stems and leaves, the extraction solvent is not particularly limited, but examples include water, alcohol (such as methanol, ethanol, n-propanol, n-butanol, etc.), and acetone. Preferably, water and/or ethanol can be used. Moreover, when obtaining the dry powder, it can be obtained by removing the solvent of the extract by a method commonly used by those skilled in the art, such as vacuum drying or spray drying.

In the present invention, barley stem and leaf powder is preferably used from the viewpoint of processability and stability, and it is particularly preferable to use pulverized powder or squeezed powder, and pulverized powder is particularly preferable. In the present invention, commercially available products may be used, or products manufactured by methods known in the art may also be used. From the viewpoint of the effect of promoting absorption of branched chain amino acids, barley stem and leaf powder preferably passes 90% or more through a sieve with an opening of 250 μm, more preferably 90% or more passes through a sieve with an opening of 150 μm. Particularly preferably, it is one that passes 90% or more through a sieve with an opening of 106 μm, and especially one that passes 90% or more through a sieve with an opening of 75 μm.

[2. Composition for promoting absorption of branched chain amino acids]
The present invention relates to a composition for promoting absorption of branched chain amino acids containing barley stems and leaves. Branched chain amino acids are amino acids that have branched aliphatic side chains. Specific examples include leucine, isoleucine, and valine. The compositions of the invention promote the absorption of branched chain amino acids, particularly leucine. Further, the composition of the present invention can exhibit excellent muscle-building effects and anti-obesity effects by promoting absorption of branched chain amino acids.

The composition for promoting absorption of branched chain amino acids of the present invention not only has an effect of promoting absorption of branched chain amino acids by containing barley stems and leaves, but also has a beautiful appearance and flavor due to its bright color. It is possible to achieve both good quality and good quality.

In the composition for promoting absorption of branched chain amino acids of the present invention, the content of barley stems and leaves is not particularly limited as long as it can solve the problems of the present invention, but for example, 0.1wt in terms of dry mass of barley stems and leaves. % or more and 100 wt% or less, preferably 1 wt% or more and 90 wt% or less, more preferably 3 wt% or more and 85 wt% or less, and even more preferably 5 wt% or more and 80 wt% or less.

The stems and leaves of barley used in the composition for promoting absorption of branched chain amino acids of the present invention have an excellent effect of promoting absorption of branched chain amino acids. For example, by using barley stems and leaves, the amount of branched chain amino acids used in combination can be absorbed into the body, and the physiological activity of the branched chain amino acids can be enhanced.

Since the composition for promoting absorption of branched chain amino acids of the present invention has an effect of promoting absorption of branched chain amino acids, it can be utilized in various forms aimed at obtaining such an effect. The composition for promoting absorption of branched chain amino acids of the present invention may be used for various purposes without special treatment.

In the solid content of the composition for promoting absorption of branched chain amino acids of the present invention, the content of barley stems and leaves is preferably 0.1% by mass or more, and 0.5% by mass or more as a lower limit in terms of dry mass. more preferably 1% by mass or more, even more preferably 10% by mass or more, particularly preferably 20% by mass or more, and the upper limit is preferably 99.9% by mass or less, more preferably 90% by mass or less, More preferably, it is 80% by mass or less. In order to fully exhibit the effect of promoting absorption of branched chain amino acids, the content of barley stems and leaves is preferably 0.1% by mass or more.

The daily usage amount of the composition for promoting absorption of branched chain amino acids of the present invention is not particularly limited, and can be set as appropriate depending on the mode of use and the content of use by the user. The amount is 1 to 6000 mg/kg, preferably 1 to 4000 mg/kg, more preferably 10 to 3000 mg/kg, and even more preferably 10 to 2000 mg/kg, based on the user's body weight. Similarly, the amount of the composition for promoting absorption of branched chain amino acids of the present invention used per time is not particularly limited, and is, for example, 0.5 to 3000 mg based on the weight of the user in terms of the mass of barley stems and leaves. /kg, preferably 1 to 2000 mg/kg, more preferably 5 to 1000 mg/kg.

Further, the amount of the branched chain amino acid absorption promoting composition of the present invention used once is, for example, 0.01 to 30 g, preferably 0.05 to 20 g, more preferably 0.01 to 30 g, preferably 0.05 to 20 g, in terms of the mass of barley stems and leaves. .1 to 10 g, particularly preferably 0.3 to 7 g. Similarly, the daily usage amount of the composition for promoting absorption of branched chain amino acids of the present invention is, for example, 0.01 to 100 g, preferably 0.05 to 70 g, more preferably The amount can be 0.5 to 50 g, particularly preferably 1 to 30 g.

The composition for promoting absorption of branched chain amino acids of the present invention may contain only barley stems and leaves, or may contain other components in addition to barley stems and leaves. Other components include, for example, various excipients, binders, lubricants, stabilizers, diluents, fillers, thickeners, emulsifiers, colorants, fragrances, additives, and the like. The content of other components can be appropriately selected depending on the usage form of the composition for promoting absorption of branched chain amino acids of the present invention.

The composition for promoting absorption of branched chain amino acids of the present invention can be utilized in various forms for the purpose of obtaining an effect of promoting absorption of branched chain amino acids. The composition for promoting absorption of branched chain amino acids of the present invention can be, for example, an oral or parenteral composition for promoting absorption of branched chain amino acids.

The form of the composition for promoting absorption of branched chain amino acids of the present invention is not particularly limited, and can be in any form. The form of the oral branched chain amino acid absorption promoting composition includes, for example, a form suitable for oral use, specifically, powder, granules, granules, tablets, liquid, paste, and hard capsules. Examples include capsule shapes such as soft capsules, caplets, and tablets.

The packaging form of the composition for promoting absorption of branched chain amino acids of the present invention is not particularly limited and can be appropriately selected depending on the dosage form, etc., but examples include blister packs such as PTP; strip packaging; heat sealing; aluminum pouches; plastic Examples include film packaging using plastic or synthetic resin; glass containers such as vials; and plastic containers such as ampoules.

The composition for promoting absorption of branched chain amino acids of the present invention is in powder form (powder form such as powder, granules, etc.), and when mixed with water and used orally, it prevents spoilage and lasts for a long time. It is preferred because it is suitable for storage. Furthermore, when the composition for promoting absorption of branched chain amino acids of the present invention is in a solid form such as a powder or a tablet, it can be mixed with water to form a liquid and used orally, as described above. However, depending on the user's preference, it may also be used orally in solid form.

Moreover, in order to fully exhibit the physiological activity of branched chain amino acids, it is preferable that the composition for promoting absorption of branched chain amino acids of the present invention contains branched chain amino acids together with barley stems and leaves. As the branched chain amino acid, the amino acid itself can be used, and proteins and peptides containing branched chain amino acids can also be used. Examples of proteins and peptides include animal proteins such as whey, casein, and silk, and vegetable proteins such as soybeans, rice, peas, almonds, cranberries, fava beans, hemp seeds, potatoes, and potato waste.

When the branched chain amino acid is derived from a protein or a peptide, its average molecular weight is not particularly limited, and is, for example, 500 to 100,000 or less, preferably 1,000 to 100,000. The concentration and amount of the branched chain amino acid added to the composition for promoting absorption of branched chain amino acids of the present invention are not particularly limited, and may vary depending on the form of the composition and the desired physiologically active effect of the branched chain amino acid. Can be set as appropriate. The recommended amount of branched chain amino acids to be used per day for an adult is, for example, 0.01 g to 100 g, preferably 0.1 g to 50 g.

When the composition for promoting absorption of branched chain amino acids of the present invention contains branched chain amino acids together with barley stems and leaves, the blending ratio of barley stems and leaves to branched chain amino acids is preferably 1:0.00001 to 50, more preferably is 1:0.00005 to 10, particularly preferably 1:0.0001 to 5.

Further, branched chain amino acids may be used in combination with the composition for promoting branched chain amino acid absorption of the present invention. In this case, the term "combined use" refers to the use of branched chain amino acids at the same time as, or before and after, the composition for promoting absorption of branched chain amino acids of the present invention. Therefore, a kit containing the composition for promoting branched-chain amino acid absorption of the present invention and a branched-chain amino acid is useful as a kit for purposes such as muscle-strengthening action and anti-obesity action. Another aspect of the present invention is a pharmaceutical composition for muscle building and anti-obesity containing barley stems and leaves. In particular, a kit containing the composition for promoting branched chain amino acid absorption of the present invention and a branched chain amino acid component is useful as a kit for the above-mentioned purposes.

The composition for promoting absorption of branched chain amino acids of the present invention, in addition to the conventional effect of promoting absorption of branched chain amino acids possessed by barley stems and leaves, contains a large amount of vitamins, minerals, etc. derived from barley stems and leaves. Using it is useful for maintaining health.

Hereinafter, the present invention will be explained in more detail with reference to Examples, but the present invention is not limited to these Examples, and the present invention can take various embodiments as long as the problems of the present invention can be solved. .

<Evaluation of leucine permeability in Caco-2 cells>
1. Sample preparation
The following were used as test substances.
(1) Ground powder of barley stems and leaves obtained by washing, cutting, drying, and crushing barley stems and leaves that are harvested before heading (more than 90% passes through a 75 μm sieve; Toyo Co., Ltd. (manufactured by Shinyakusha) was used. 24 mg of ground barley stem and leaf powder was placed in a tube, 1 mL of buffer was added, and the tube was vortexed. Thereafter, centrifugation was performed at 15,000×g for 5 minutes at room temperature, and the supernatant was collected. After filter sterilization, this was diluted and used as a test substance.
(2) L-leucine (manufactured by Nacalai Tesque: 20327-46) was used as the branched chain amino acid. 13.1 mg of L-leucine was placed in a tube, and 1 mL of buffer was added to dissolve it (100 mM). After filter sterilization, this was diluted and used as a test substance.
The final concentration of each test substance is shown below.

2. Cell culture and addition of test substance (1) Human colon cancer-derived cells (Caco-2) were cultured in a 75 cm ² flask at 37° C. in a 5% CO ₂ incubator. The medium used was 10% FBS-DMEM.
(2) 600 μl of medium was added to a 24-well plate, and the insert well was transferred.
(3) 100 μl of cells suspended by trypsin treatment were seeded into insert wells from a 75 cm ² flask at a cell density of 4.0×10 ⁴ cells/well.
(4) Preculture was carried out in a 5% CO ₂ incubator at 37° C. for 14 days, during which time the medium was replaced every 2 to 3 days. When replacing the medium before sample addition, the outside of the insert well (well on the basement membrane side) was replaced with 1000 μl, and the inside of the insert well (well on the luminal side) was replaced with 350 μl of 10% FBS-DMEM.
(5) The electrical resistance value was measured using Millicell ERS-2, and wells with a resistance of 1000Ω or more were used. Furthermore, the test substances were divided into groups such that the average electrical resistance value of each group to which the test substance was added was the same.
(6) After washing the outside of the insert well three times and the inside three times with 1000 μL of buffer each time, it was transferred to a 24-well plate containing 1000 μL of buffer, and 350 μL of buffer was added to the insert well.
(7) Cultured in a CO ₂ incubator for 30 minutes to acclimatize the cells to the buffer.
(8) After removing 175 μL of the buffer inside the insert well, 175 μL each of the test substance solution prepared at twice the concentration was added and cultured in a CO ₂ incubator for 2 hours.
(9) After culturing, the entire sample of the basement membrane side well was collected into a 1.5 mL tube.

3. Measurement of leucine concentration by high performance liquid chromatography After dispensing 500 μL of the sample from the basement membrane side well collected in 2 (9) above into a tube, the sample was dried using a centrifugal evaporator. Furthermore, after drying 400 μL of the sample in the same tube, 200 μL of 100 mM HCl was added and dissolved by applying ultrasound for 10 minutes. The leucine concentration of the obtained concentrate was measured by high performance liquid chromatography. The measurement results were applied to the following formula to calculate the leucine permeability.

The results are shown in Figure 1. In Comparative Example 1, in which only leucine was administered, the leucine permeability was 1.32%, whereas in Example 1, in which ground barley stem and leaf powder was used, it was 3.86%, about three times as high. An improvement in leucine permeability was observed. Therefore, it was shown that the composition of the present invention has an excellent effect of promoting absorption of branched chain amino acids.

Granules consisting of the following ingredients were manufactured. By ingesting the obtained granules twice a day, 3 g per time suspended in water, the absorption of branched chain amino acids is promoted by the crushed barley grass powder, which has an excellent muscle-building effect and anti-obesity effect. Effects can be obtained.

Granules consisting of the following ingredients were manufactured. By ingesting the obtained granules three times a day by suspending 3g in water each time, the crushed powder of barley grass promotes the absorption of branched chain amino acids, which has an excellent muscle-building effect and anti-obesity effect. Effects can be obtained.

Granules consisting of the following ingredients were manufactured. By ingesting the obtained granules once a day by suspending 5g in water, the crushed powder of barley grass promotes the absorption of branched chain amino acids, which has an excellent muscle-building effect and anti-obesity effect. Effects can be obtained.

Granules consisting of the following ingredients were manufactured. By ingesting the obtained granules twice a day, 3 g per time suspended in water, the absorption of branched chain amino acids is promoted by the crushed barley grass powder, which has an excellent muscle-building effect and anti-obesity effect. Effects can be obtained.

Since the oral composition of the present invention has an excellent effect of promoting absorption of branched chain amino acids, it has high industrial utility.

Claims (2)

A composition for promoting absorption of branched chain amino acids, characterized by containing barley grass. The composition for promoting absorption according to claim 1, wherein the branched chain amino acid is leucine.