JP7407447B2 - Compound treatment system that causes less physical damage to insects - Google Patents

Description

The present invention relates to a compound treatment system that causes less physical damage to insects such as minute sap-sucking flying pests.

The tobacco whitefly is an agricultural pest that sucks the sap and transmits plant disease viruses to more than 600 types of plants, such as tomatoes and cucumbers, causing significant economic damage such as reduced yields and decreased commercial value. Particularly in recent years, due to the spread of insecticide-resistant strains, there has been an urgent need to develop new, effective and safe pest control methods to replace conventional pesticides. When screening seed compounds that can be used as new pest control agents, experiments using living insects are essential.

Patent Document 1 discloses a cylindrical body having a device connection port for connecting to a suction device at one end and a pest suction port for sucking pests at the other end, and an internal space of the cylindrical body. A pest trap has been proposed that includes a deodorizing filter attached to the inside of a cylindrical body so as to partition the device connection port side and the pest suction port side.

Patent Document 2 describes an attraction-inhibiting activity test device that can determine the effect of the airborne concentration of a drug on the attracting behavior of flying pests. The attraction/inhibition activity test device includes a drug holder installation section in which a drug holder in which a drug having attraction/inhibition activity against flying pests is held on a base material, a test section in which a flying pest can be accommodated, and a test section in which a drug holder can be installed. It has an airflow generating means capable of generating an airflow from the upstream side to the downstream side with respect to the section.

JP2008-263875A Japanese Patent Application Publication No. 2008-208060

The tobacco whitefly is tiny, less than 0.8 mm long, and can fly. For this reason, it has been difficult to handle when screening for drugs such as insecticides. For example, when transferring tobacco whiteflies from a rearing container to a sap container, giving them a drug to be evaluated, and then transferring them to a test container, the individuals may die during the experiment due to the effects of static electricity and physical damage caused by handling. There were many things that went wrong. Furthermore, due to such difficulty in handling, conducting in-vivo experiments on a large number of candidate compounds requires a great deal of time and human cost, which is a major problem in screening for new pest control agents.

SUMMARY OF THE INVENTION An object of the present invention is to provide a technique that suppresses physical damage to the insects and facilitates the transportation of the sucking flying insects.

In order to solve the above problems, the compound processing system of the present invention includes:
A hollow member having a suction port formed at one end for sucking in the succulent flying pests, and a connecting part for connecting to an airflow generation source at the other end, the hollow member having a suction port for sucking in the succulent flying pests. a nozzle section provided with a filter that prevents passage;
a cylindrical main body portion whose first end portion is connected to the suction port side end portion of the nozzle portion;
A cylindrical member connected to a second end opposite to the first end of the main body,
A film is provided at the end of the cylindrical member that closes the hollow portion of the cylindrical member, and a chemical solution containing a compound to be administered to the sap-sucking flying pest is provided in the hollow portion partitioned by the film. a compound holding part that holds;
Equipped with.

The compound processing system is configured such that the compound holding part is removably connected to the second end of the main body part,
The second end of the main body portion may include a connection portion that can be connected to a test container for transporting the sap-sucking flying pest after administering the compound when the compound holding portion is removed.

In the compound treatment system, the sap-sucking flying pest may be tobacco whitefly.

In order to solve the above problems, the compound administration method of the present invention includes:
A hollow member having a suction port formed at one end for sucking in the succulent flying pests, and a connecting part for connecting to an airflow generation source at the other end, the hollow member having a suction port for sucking in the succulent flying pests. Connecting a nozzle portion provided with a filter that prevents passage to the airflow generation source, generating an airflow from the suction port toward the filter side, and sucking in the sap-sucking flying pest;
Inserting the suction port side end of the nozzle part into the hollow part of the first end of the cylindrical main body part, and connecting the first end of the main body part and the nozzle part;
A cylindrical compound holding part connected to a second end of the main body opposite to the first end, the cylindrical member being connected to the end connected to the main body. By holding a chemical solution containing a compound to be administered to the sap-sucking flying pests in the hollow part partitioned by the film of the compound holding part provided with a film that closes the hollow part, a step of causing a sap-sucking flying pest to sap the chemical solution;
including.

In the method for administering a compound, after the step of causing the suction-sucking flying pest to suck the chemical solution, the compound holding part is removed from the second end of the main body, and the second end of the main body is attached to a test container. The method may further include the step of connecting the sap-sucking flying pest to the test container.

In the method for administering a compound, the sap-sucking flying pest may be the tobacco whitefly.

INDUSTRIAL APPLICATION This invention can provide the technique which suppresses the physical damage to the said insect, and facilitates transport, when transporting a sap-sucking flying pest.

FIG. 1 is a schematic configuration diagram of a compound processing system. Exploded perspective view of the nozzle part, It is an exploded perspective view showing a main body part and a compound holding part. FIG. 2 is a diagram showing the steps of a compound administration method.

DESCRIPTION OF THE PREFERRED EMBODIMENTS A compound processing system 1 according to an embodiment of the present invention (hereinafter referred to as an embodiment) will be described below with reference to the drawings. The configurations of the following embodiments are illustrative, and the present invention is not limited to the configurations of the embodiments. FIG. 1 is a schematic diagram of a compound processing system 1. As shown in FIG.

<System configuration>
As shown in FIG. 1, the compound processing system 1 includes a breeding container 2, a compound administration device 3, a vacuum pump (airflow generating section) 4, a rack 5, and a test container 6.

The rearing container 2 houses a succulent flying pest 7 and a plant 8 for the flying pest 7 to suck sap from. In this embodiment, an inverted plastic wide-mouth bottle is used as the rearing container 2. That is, the bottle body 21 of the rearing container 2 is arranged with the opening 21A facing downward, and the lid 22 that is screwed into the screw opening forming the opening 21A is used as the bottom. Note that the rearing container 2 is not limited to the configuration shown in FIG. 1, and may be any container as long as it can accommodate and rear the flying pests 7. The bottle body 21 of this embodiment is made of a transparent material, so that the flying pests 7 can be observed from the outside and the plants 8 can be illuminated with light. A hole 21B penetrating the inside and outside of the container is formed on the side surface of the bottle body 21 for use in collecting flying pests 7, and a cover 21C that can be opened and closed is attached. In addition, a hole 21D for letting air pass is formed, and this hole is covered with a mesh 21E whose opening is smaller than the size of the flying pest 7, and cannot be opened or closed. In this embodiment, the succulent flying pest 7 is, for example, the tobacco whitefly. The flying pests 7 are not limited to this, and may be whiteflies such as the whitefly, or other sap-sucking flying pests.

The compound administration device 3 has a nozzle section 31, a main body section 32, and a compound holding section 33. FIG. 2 is an exploded perspective view of the nozzle section 31. The nozzle part 31 is a hollow member having a suction port 311 formed at one end for sucking in the sap-sucking flying pests, and a connecting part 312 for connecting to an airflow generation source at the other end. Further, the nozzle portion 31 is provided with a filter 317 in the hollow portion thereof to prevent the flying pests 7 from passing through. The material of the nozzle portion 31 is, for example, plastic. The material of the nozzle part 31 is not particularly limited, but it is preferably transparent so that the flying pests 7 sucked can be visually recognized. As shown in FIG. 2, the nozzle part 31 includes a part 313 having a tapered shape on the suction port side, and a part 316 that is fitted into the hollow part 315 from an end 314 of the part 313 on the side opposite to the suction port 311. have. A filter 317 is attached to the tip side of the part 316. The end of the part 316 opposite to the filter 317 is a connecting portion 312 that is connected to the vacuum pump 4 .

The filter 317 allows the air sucked by the vacuum pump 4 to pass through, but prevents the flying pests 7 from passing through, and keeps the flying pests 7 sucked through the suction port 311 within the hollow portion 315 . The filter 317 of this embodiment is a mesh whose opening is smaller than the size of the flying pest 7. Note that the filter 317 is not limited to mesh, and may be made of cloth, lattice, nonwoven fabric, cotton, sponge, or the like. In this embodiment, the volume of the hollow portion 315 on the side of the suction port 311 partitioned by the filter 317 is 1000 μl. The volume of the hollow portion 315 is not limited to this, and may be set depending on the size and number of the flying pests 7 to be sucked.

FIG. 3 is an exploded perspective view showing the main body part 32 and the compound holding part 33. The main body part 32 is a cylindrical member whose first end 321 is connected to the suction port side end of the nozzle part 31 . The compound holding part 33 is a cylindrical member that connects to a second end 322 of the main body 32 opposite to the first end 321, and has an end 331 connected to the main body 32. A film 332 is provided to close the hollow portion of the cylindrical member. The compound holding part 33 is removably connected to the main body part 32. The compound holding section 33 holds a chemical solution containing a compound to be administered to the succulent flying pest 7 in a hollow portion partitioned by the film 332 . The film 332 is, for example, a paraffin film. However, the film 332 is not limited to this, and as described later, the film 332 may be made of any other material as long as the flying pest 7 can pierce the mouth needle and absorb the medicinal liquid, and for example, the film 332 may be formed of other materials such as polyethylene film. .

The main body portion 32 and the compound holding portion 33 have approximately the same diameter, and in this embodiment, their outer diameter is 12 mm. Further, the length of the main body portion 32 is 50 mm, and the length of the compound holding portion 33 is 10 mm. Note that these sizes are not particularly limited, and can be set depending on the size and number of flying pests 7 to be captured, the amount of chemical solution, etc.

The vacuum pump (airflow generation section) 4 is connected to the connection section 312 of the nozzle section 31 via a tube 41, and generates an airflow from the suction port 311 side to the connection section 312 side by suctioning air. The flying pest 7 is sucked. Note that the airflow generating section is not limited to the vacuum pump 4, but may be a syringe, a pipetter, or the like.

The rack 5 is a stand that holds the compound administration device 3 in an upright state, and is provided with a row of vertical holes 51 into which the compound administration device 3 is inserted.

The test container 6 is a container for accommodating the flying pest 7 after administering the compound and the plant 8 for the flying pest 7 to suck sap, and for confirming the result of administering the compound. In this embodiment, similarly to the rearing container 2, an inverted plastic wide-mouth bottle is used as the test container 6. That is, the bottle body 61 of the test container 6 is arranged with the opening 61A facing downward, and the lid 62 that is screwed into the screw opening forming the opening 61A is used as the bottom. Note that the test container 6 is not limited to the configuration shown in FIG. 1, and may be any container as long as it can accommodate and raise the flying pests 7. The bottle body 61 of this embodiment is made of a transparent material, so that the flying pests 7 can be observed from the outside, and the plants 8 can be illuminated with light. A hole 61B penetrating the inside and outside of the container is formed on the side surface of the bottle body 61 for use in collecting flying pests 7, and a cover 61C that can be opened and closed is attached. In addition, an air hole 61D for ventilation is formed, which is covered with a mesh 61E whose opening is smaller than the size of the flying pest 7. A hole 61F for inserting the compound administration device 3 is formed in the upper surface of the bottle body 61, and an openable/closeable cover 61G is attached.

<Compound administration method>
FIG. 4 is a diagram showing the steps of the compound administration method.

In step S1, a predetermined number of flying pests 7 are captured from the breeding container. In this case, the tube 41 of the vacuum pump 4 is connected to the connection part 312 of the nozzle part 31, the nozzle part 31 is inserted through the hole 21B on the side of the rearing container 2, the suction port 311 is brought close to the flying pest 7, and the flying pest 7 is removed. It is captured by suction.

In step S2, the nozzle section 31 that has captured the flying pest 7 is connected to the main body section 32, and the tube 41 is removed from the connection section 312. At this time, the flying pests 7 may come out of the nozzle part 31 more easily by cutting off the tapered tip of the suction port 311 to widen the opening and then connecting it to the main body part 32. By connecting the nozzle part 31 to the main body part 32 in this way, the flying pests 7 can be trapped in the hollow parts of the nozzle part 31 and the main body part 32, and the filter 317 of the nozzle part 31 and the film of the compound holding part 33 332.

In step S3, the connecting portion 312 of the compound administering device 3 is inserted into the vertical hole 51 of the rack 5, so that the compound administering device 3 is arranged in an upright position, and a drug solution containing a compound (for example, a sugar solution such as sucrose) is placed in the compound holding portion 33. A solution of a substance capable of attracting flying pests) is put therein, and the upper end is sealed with a film 324 or a cap. Note that the compound holding section 33 filled with a chemical solution containing a compound and having its upper end sealed with a film 324 or the like may be connected to the main body section 32 in advance. In this way, the compound administration device 3 is left standing and standing still, and after a while, the flying pests 7 naturally move to the top, insert their mouth needles into the film 332, and suck up the sucrose solution containing the compound. At this time, the flying pests 7 may be guided to the upper part by arranging a cap or a light source in a color (for example, yellow) that the flying pests 7 like.

In step S4, the sucrose solution is collected from the compound holding part 33, and the compound administration device 3 is stood upright with the compound holding part 33 facing down, and left standing. After a while, the flying pest 7 moves to the upper part. At this time, the flying pests 7 may be guided to the upper part of the main body part 32 by placing paper or a light source of a color (for example, yellow) that the flying pests 7 like in the upper part of the main body part 32 (on the side of the connecting part 312).

In step S5, the compound holding part 33 is removed from the main body part 32, and the compound administration device 3 is inserted into the hole 61F at the top of the test container 6, so that the hollow part in the compound administration device 3 and the internal space of the test container 6 are connected. The compound administration device 3 and the test container 6 are connected in a communicating state. After a while, the flying pests 7 naturally migrate from within the compound administration device 3 to the plants 8 within the test container 6. Note that when connecting the main body 32 of the compound administration device 3 to the test container 6, an O-ring or the like is provided at a predetermined distance rearward from the tip of the main body 32 to form an enlarged diameter portion 323. It may be configured such that the enlarged diameter portion 323 abuts against the top surface of the bottle body 61 at a position inserted a predetermined distance into the top surface of the bottle body 61, and the compound administration device 3 is held.

<Effects of embodiment>
As described above, according to the present embodiment, the nozzle section 31 that sucks the flying pests 7 is connected to the main body section 32, and the compound holding section 33 is left standing with the compound holding section 33 facing upward, so that the flying pests 7 are naturally absorbed. Move to the compound holding section 33 side. Previously, the collected insects were cooled in a refrigerator or anesthetized with _CO2 to suppress their movement to prevent them from escaping, and then transferred to a suction container, but this method does not require this step. Therefore, it is possible to move the flying pest 7 in a short period of time without damaging it.

Furthermore, since the compound holding part 33 into which the sucrose solution is placed and the main body part 32 in which the flying pests 7 are held are separated by a film 332, the solution can be replaced or replenished without missing the flying pests 7, and the insects can be spit out. It is possible to collect the liquid.

Furthermore, in this embodiment, in step S4, when the sucrose solution is collected from the compound holding part 33 and left standing with the compound holding part 33 facing down, the flying pests 7 move to the upper part and the film 332 of the compound holding part 33 The compound holding part 33 is removed at a distance from the test container 6, and the compound administration device 3 is connected to the test container 6. As a result, the flying pests 7 naturally move from the compound administration device 3 to the plants 8 in the test container 6. Conventionally, the flying pests 7 were sucked out from the sap container with a sucking tube and forcibly transferred onto the plant, whereas in this method the flying pests 7 are moved naturally, reducing the physical damage caused by the movement. be able to.

1 Compound treatment system 2 Breeding container 3 Compound administration device 4 Vacuum pump 5 Rack 6 Test container 7 Flying pests 8 Plant 21 Bottle body 21A Opening 21B Hole 21C Cover 21D Air hole 21E Mesh 22 Lid 31 Nozzle part 32 Main body part 33 Compound retention Part 311 Suction port 312 Connection part 313 Part 314 End part 315 Hollow part 316 Part 317 Filter 321 End part 322 End part 331 End part 332 Film 41 Tube 51 Vertical hole 61 Bottle body 61A Opening part 61B Hole 61C Cover 61D Air hole 61E Mesh 61F Hole 61G Cover 62 Lid 324 Film

Claims (6)

A hollow member having a suction port formed at one end for sucking in the succulent flying pests, and a connecting part for connecting to an airflow generation source at the other end, the hollow member having a suction port for sucking in the succulent flying pests. a nozzle section provided with a filter that prevents passage;
a cylindrical main body portion whose first end portion is connected to the suction port side end portion of the nozzle portion;
A cylindrical member connected to a second end of the main body opposite to the first end, and a hollow portion of the cylindrical member at the end connected to the main body. a compound holding part for holding a chemical solution containing a compound to be administered to the sap-sucking flying pest in the hollow part partitioned by the film;
A compound treatment system for sap-sucking flying pests. the compound holding portion is removably connected to the second end of the body portion;
2. The method according to claim 1, wherein the second end of the main body includes a connection part that can be connected to a test container for transporting the sap-sucking flying pest after administering the compound when the compound holding part is removed. The described compound processing system. The compound treatment system according to claim 1 or 2, wherein the sap-sucking flying pest is tobacco whitefly. A hollow member having a suction port formed at one end for sucking in the succulent flying pests, and a connecting part for connecting to an airflow generation source at the other end, the hollow member having a suction port for sucking in the succulent flying pests. Connecting a nozzle portion provided with a filter that prevents passage to the airflow generation source, generating an airflow from the suction port toward the filter side, and sucking in the sap-sucking flying pest;
inserting the suction port side end of the nozzle part into the hollow part of the first end of the cylindrical main body part, and connecting the first end of the main body part and the nozzle part;
A cylindrical compound holding part connected to a second end of the main body opposite to the first end, the cylindrical member being connected to the end connected to the main body. By holding a chemical solution containing a compound to be administered to the sap-sucking flying pest in the hollow part partitioned by the film of the compound holding part provided with a film that closes the hollow part, a step of causing a sap-sucking flying pest to sap the chemical solution;
A method for administering a compound to a sap-sucking flying pest containing. After the step of causing the liquid-sucking flying insect to suck the chemical solution, the compound holding part is removed from the second end of the main body, the second end of the main body is connected to the test container, and the compound holding part is removed from the second end of the main body. 5. The method for administering a compound according to claim 4, further comprising the step of transferring a sap-sucking flying pest to the test container. The method for administering a compound according to claim 4 or 5, wherein the sap-sucking flying pest is tabacum whitefly.

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