JP7541780B2 - Oral composition for improving bad breath - Google Patents

Description

The present invention relates to an oral composition, specifically an oral composition for improving bad breath that can remove bad breath components and improve bad breath.

In modern society, with industrialization and Westernization, the causes and symptoms of oral cavity-related diseases have become more diverse due to changes in lifestyle and dietary habits, and oral cavity-related diseases and problems have been reported as serious social issues.

The oral cavity is a very important organ for breathing and ingesting food, but it is also an organ that is constantly exposed to the external environment through air and food. Therefore, if you do not keep your mouth clean by brushing your teeth, plaque and tartar will form around the teeth and gums, which can cause various diseases.

The World Health Organization (WHO) recognizes oral diseases such as dental caries, periodontal disease, bad breath and oral pain as one of the major health problems that must be solved in the future. According to a WHO report on dental diseases, 60-90% of adolescents and 60% of adults worldwide suffer from dental caries and periodontal disease, and 15-20% of middle-aged adults aged 35-44 have severe periodontal disease. These oral diseases are not only the main cause of tooth loss, but are also reported to be potential risk factors for diseases such as cardiovascular disease, bacterial pneumonia, low birth weight, diabetic complications and osteoporosis.

As mentioned above, the human oral cavity is an organ that is constantly exposed to the external environment through air and food. With high humidity and a stable body temperature, it is an ideal environment for food to spoil and for bacteria to grow. More than 700 different types of bacteria are normally present in the human oral cavity, which consists of normal bacteria and pathogenic bacteria. While normal bacteria prevent the growth of pathogenic bacteria and maintain oral health, it is known that most of the bacteria that cause oral diseases exist in plaque, a bacterial film formed between the teeth and gums. Pathogenic bacteria produce toxins and acids from plaque, and the toxins produced by pathogenic bacteria invade periodontal tissues and cause inflammation of the gums, destruction of the alveolar bone, and infection, and the acids produced by pathogenic bacteria dissolve the surface of the teeth, causing caries and may also act as a cause of bad breath.

In relation to the above-mentioned cause of bad breath, awareness of bad breath is gradually increasing as the scope and activity of interpersonal relationships increases with social development and hygiene concepts develop, and as a result, the number of consumers who actively try to care for their bad breath is on the rise. In response to these changes, various research has been conducted both domestically and internationally, including the implementation of treatments that are effective in removing bad breath and the incorporation of medicines into toothpaste, mouthwash, capsules, adhesives, etc.

The causes of bad breath can be broadly divided into two categories: intraoral causes and extraoral causes. Intraoral causes typically include oral microorganisms or oral diseases such as untreated caries and periodontal disease. Extraoral causes include respiratory diseases, gastrointestinal diseases, and a variety of other causes such as hunger, menstruation, smoking, and alcohol consumption.

Among the above causes, oral causes of bad breath, which is the main cause, are known to occur when enzymes in the mouth use food debris in saliva, microorganisms, oral epithelium, detached oral mucosa and connective tissue as protein sources and break them down to produce volatile sulfides. Volatile sulfides, which are the main components that cause bad breath, include hydrogen sulfide, methyl mercaptan and dimethyl mercaptan, and in addition to these volatile sulfides, other compounds include volatile amine compounds such as triethylamine, aldehydes, fatty acids and ammonia.

Looking at conventional technologies related to removing bad breath, research has been conducted mainly on toothpaste and mouthwash that contain antibacterial agents against bacteria that cause bad breath and masking ingredients against substances that cause bad breath. However, these preparations have some limitations in terms of use in terms of expressing and maintaining the effect of removing bad breath. For example, toothpaste has the disadvantage that the active ingredients cannot be maintained after brushing the teeth. To compensate for this disadvantage, attempts have been made to increase the drug delivery effect by applying polymers, but in order to use an ointment-like dosage form such as conventional toothpaste, one must brush one's teeth with a toothbrush and rinse one's mouth with water, which still leaves the disadvantage of being limited in terms of location. In addition, research has been conducted on dosage forms such as mouth sprays and breath films. However, mouth sprays and breath films have the problem that their efficacy lasts for a short time, limiting the experience of their efficacy, and in particular, breath films have phenomena such as shrinkage and aggregation of the manufactured film due to temperature and humidity, which adds to distribution problems.

In addition, to suppress bad breath occurring in the oral cavity, research is being conducted to remove substances that cause bad breath, such as volatile sulfides such as hydrogen sulfide, methyl mercaptan, and dimethyl mercaptan, which are components of the breakdown of protein waste. This is considered to be more effective than controlling the microorganisms involved in bad breath, the effectiveness of which has been limited, and in this regard, there is an increasing need for research into methods that can improve bad breath through effective removal of bad breath.

The present invention aims to provide an oral composition that, unlike existing toothpastes, can improve bad breath by effectively removing bad breath.

As described above, the present invention provides an oral composition for improving bad breath that can effectively remove decomposition components of protein waste products that generate volatile sulfides, such as hydrogen sulfide, methyl mercaptan, and dimethyl mercaptan, which are the causes of bad breath.

Specifically, the oral composition for improving bad breath of the present invention may be an oral composition for improving bad breath that contains chitosan and green tea extract as active ingredients.

The chitosan may have a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da.

In this respect, the oral composition for improving bad breath may be an oral composition for improving bad breath that contains chitosan and green tea extract as active ingredients, and the chitosan may be characterized in that it has a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da.

The chitosan content may preferably be 0.01 to 0.05% (w/w) based on the weight of the oral composition for improving bad breath. In view of this, the oral composition for improving bad breath may have a chitosan content of 0.01 to 0.05 parts by weight based on 100 parts by weight of the oral composition for improving bad breath.

The green tea extract may impart astringency to the entire oral composition due to the content of catechins contained in the green tea extract, reducing the palatability of the entire oral composition, so the content of the green tea extract is affected by the content of catechins contained in the green tea extract.

In view of this, the content of the green tea extract can be adjusted based on the content of catechins contained in the green tea extract, and therefore the content of the green tea extract can be adjusted so that the content of catechins contained in the green tea extract is 0.1 to 0.2% (w/w) based on the weight of the oral composition for improving breath.

Therefore, the content of the green tea extract may be preferably 0.1 to 0.2 parts by weight based on the content of catechin contained in the green tea extract, based on 100 parts by weight of the oral composition for improving bad breath.

The oral composition for improving bad breath may further contain one or more additional components selected from the group consisting of polyols, polymers, bactericides, solubilizers, antibacterial components, active components for removing bad breath, components for preventing gum disease, pH regulators, preservatives, antiseptics, flavoring agents, sweeteners, flavorings, dyes, and solvents.

The oral composition for improving bad breath of the present invention uses the natural products chitosan and green tea extract as its main ingredients, minimizing the use of synthetic compounds that are harmful to the human body, making it not only safe, but also highly palatable, without causing inconvenience in use such as astringency, while still providing excellent bad breath removal effects, and thus improving the consumer's usability and satisfaction.

1 shows the results of measuring the bad breath of a subject before and after use of the present invention using an odor measuring device according to one embodiment of the present invention. 1 is a photograph showing the measurement results of the effect of removing halitosis-inducing substances for reducing bad breath of an oral composition of the present invention according to one embodiment of the present invention.

The present invention relates to an oral composition for improving bad breath, which comprises chitosan and green tea extract as active ingredients,
The chitosan has a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da;
The green tea extract contains catechins,
The content of the chitosan is 0.01 to 0.05 parts by weight based on 100 parts by weight of the oral composition for improving bad breath,
The content of the green tea extract is based on the content of catechin contained in the green tea extract, and based on 100 parts by weight of the oral composition for improving bad breath, the content of the catechin is 0.1 to 0.2
The present invention relates to an oral composition for improving bad breath, characterized in that

In order to explain the present invention in detail, preferred embodiments of the present invention will be described below. The following examples are provided to illustrate the present invention, and the scope of the present invention is not limited in any way by the following examples. Modifications that are commonly made in the industry are also included within the scope of the present invention.

The present invention relates to an oral composition for improving bad breath that contains chitosan and green tea extract as active ingredients.

The oral composition for improving bad breath contains chitosan and green tea extract as active ingredients, and the chitosan may have a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da.

The oral composition for improving bad breath may have a chitosan content of 0.01 to 0.05 parts by weight based on 100 parts by weight of the oral composition for improving bad breath.

The chitosan content may preferably be 0.01 to 0.05% (w/w) based on the weight of the oral composition for improving bad breath.

The green tea extract may contain catechin, and the content of the green tea extract may be 0.1 to 0.2 parts by weight based on the content of catechin contained in the green tea extract, based on 100 parts by weight of the oral composition for improving bad breath.

The green tea extract may contain catechin, and the content of catechin contained in the green tea extract may be preferably 0.1 to 0.2% (w/w) based on the weight of the oral composition for improving bad breath.

The oral composition for improving bad breath may further contain one or more additional components selected from the group consisting of polyols, polymers, bactericides, caries inhibitors, active ingredients for removing bad breath, pH adjusters, preservatives, antiseptics, flavoring agents, sweeteners, flavorings, dyes, and solvents.

Unless otherwise specified in this specification, the average molecular weight means the molecular weight of a polymer compound that is a mixture of multiple molecular species with different molecular weights, i.e., the average molecular weight of multiple molecular species with different molecular weights. The average molecular weight explicitly indicates the method of taking the average, such as the number average molecular weight or the weight average molecular weight.

Unless otherwise specified in this specification, a composition is meant to include not only a product containing the specified ingredients in the specified amounts, but also a product resulting directly or indirectly from the combination of the specified ingredients in the specified amounts.

Unless otherwise specified in this specification, an effective amount means an amount of the extract or composition of the present invention that is effective to produce the desired therapeutic, mitigating, suppressive or preventive effect.

Unless otherwise specified in this specification, the term "solubilizing agent" refers to a surfactant used to solubilize substances that are not soluble in water. The surfactant may be an anionic surfactant, an amphoteric surfactant, or a nonionic surfactant, and examples thereof include an anionic surfactant such as an amide-based nonionic surfactant, sodium lauryl sulfate, a polyoxyethylene-polyoxypropylene copolymer (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, or a taurate-based surfactant, or a betaine-based amphoteric surfactant.

The inventors of the present invention focused on controlling microorganisms that generate decomposition components of protein waste products in order to remove volatile sulfides such as hydrogen sulfide, methyl mercaptan, and dimethyl mercaptan, which are the causative substances of bad breath, in order to remove bad breath. They noticed that the effect of removing bad breath was limited despite the addition of antibacterial substances derived from natural products and antibacterial substances derived from chemical compounds to existing oral compositions for improving bad breath. They then conducted research into oral compositions that can effectively remove protein waste products by focusing on the protein waste products themselves rather than on controlling microorganisms.

As a result of conducting these studies based on natural products whose safety has been verified, rather than artificial compounds whose safety is an issue, it was confirmed that when chitosan and green tea extract are used in combination, protein waste products can be easily removed even by simply gargling, and that the effectiveness of removing such protein waste products is affected by the molecular weight of chitosan and that the amount of green tea extract added also affects the effectiveness of removing waste products. It was also confirmed that the amount of chitosan added and the green tea extract, specifically the amount of catechin contained in the green tea extract, affect the palatability of the entire oral composition. Based on the above experimental results, it was confirmed that the most suitable case is one in which the weight-average molecular weight (Mw) of chitosan is 11,170 Da, the chitosan content is 0.05% (w/w), and the green tea extract content is 0.1 to 0.2% (w/w) based on the catechin content of the green tea extract. The oral composition for improving bad breath, which was developed based on the above results, uses the natural products chitosan and green tea extract as its main ingredients, minimizing the use of synthetic compounds that are harmful to the human body, making it safe. It also has excellent bad breath removal effects, is not inconvenient to use due to its astringent taste, and is highly palatable, improving the consumer's usability and satisfaction, and solving the problems of existing oral compositions for improving bad breath. Based on this, the present invention was completed.

The present invention will be explained in more detail below.

The present invention relates to a paste-type oral composition for improving bad breath that maintains a constant level of viscosity and ensures an excellent feel when used, such as a smooth feel, without using synthetic surfactants, in order to solve the problems associated with existing paste-type toothpastes that use synthetic surfactants.

The oral composition for improving bad breath of the present invention relates to an oral composition for improving bad breath that contains chitosan and green tea extract as active ingredients.

The chitosan may have a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da, preferably 10,000 Da to 13,000 Da, more preferably 11,150 Da to 11,200 Da, and even more preferably 11,170 Da, from the viewpoints of the effect of removing protein waste products and the effect of inhibiting the production of volatile sulfides such as hydrogen sulfide, methyl mercaptan, and dimethyl mercaptan that cause bad breath.

In this respect, the oral composition for improving bad breath may be an oral composition for improving bad breath that contains chitosan and green tea extract as active ingredients, and the chitosan may be characterized in that it has a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da.

Chitosan is a natural polymeric substance obtained by deacetylating chitin, which is found in the shells of crustaceans such as crabs and shrimp, by treating it with high temperature and strong alkali. Chitosan is a substance that has various physiological functions such as antibacterial activity, antifungal activity, and immune activity enhancing effects.

The chitosan inhibits the growth of oral bacteria to prevent gum disease, and at the same time, the adsorption and coagulation of the chitosan can maintain oral hygiene. The chitosan has an acetylamino group with very strong hydrogen bonds in the molecule, so it is insoluble in water, but when the chitosan is reduced to a low molecular weight, for example, a molecular weight of 10,000 to 70,000 Da, it becomes water-soluble. The chitosan is preferably a water-soluble chitosan that is soluble in water, but is a high molecular weight chitosan with excellent effects, for example, carboxyethyl chitosan (N-carboxyethyl chitosan) obtained by binding a carboxyethyl group to normal chitosan (water-insoluble chitosan) obtained by deacetylating chitin.

The chitosan content may be preferably 0.01 to 0.05% (w/w) based on the weight of the oral composition for improving bad breath, in terms of the overall preference and usability of the oral composition.

In view of this, the oral composition for improving bad breath may have a chitosan content of 0.01 to 0.05 parts by weight based on 100 parts by weight of the oral composition for improving bad breath.

The green tea extract may impart astringency to the entire oral composition due to the content of catechins contained in the green tea extract, reducing the palatability of the entire oral composition, so the content of the green tea extract is affected by the content of catechins contained in the green tea extract.

In view of this, the content of the green tea extract can be adjusted based on the content of catechins contained in the green tea extract, and therefore the content of the green tea extract can be adjusted so that the content of catechins contained in the green tea extract is 0.1 to 0.2% (w/w) based on the weight of the oral composition for improving breath.

Therefore, the content of the green tea extract may be preferably 0.1 to 0.2 parts by weight based on the content of catechin contained in the green tea extract, based on 100 parts by weight of the oral composition for improving bad breath.

In the present invention, green tea extract means an extract of Camellia sinensis leaves.

The tea plant is an evergreen tree or evergreen broad-leaved shrub belonging to the family Camellia, and its scientific name is Camellia sinensis. It is known to be native to the mountainous region on the border between Tibet and Sichuan Province in China, and grows naturally in tropical, subtropical and temperate regions. It is currently found mainly in Korea, Japan, China, India and Southeast Asia. There are many varieties of the tea plant, and these varieties differ greatly in shape. The small-leaf variety, which is mainly cultivated in China and Japan, is a shrub that grows to a height of about 2m to about 3m even in the wild, and the large-leaf variety in the Assam region of India is a tall tree that reaches a height of about 15m to about 30m.

The leaves of the tea plant vary greatly depending on the variety and location, but are generally long ovals measuring 6-20cm in length and 3-4cm in width, with blunt sawtooth edges and pointed tips and bottoms. The leaves are hard and somewhat thick, with a glossy surface. The color of the leaves varies depending on the variety, and there are also variations in wrinkles. The undersides of young leaves and shoots have soft hairs. In Korea, the wild species that grows on Jiri Mountain is the Chinese small-leaf variety, and for cultivated tea, the Japanese Yabukita variety, an improved version of the Chinese small-leaf variety, is mainly cultivated.

The green tea extract, i.e., tea plant leaf extract, may be one produced according to a conventional method for producing plant extracts. More specifically, it may be one obtained by subjecting tea plant leaves from which impurities have been removed, crushed tea plant leaves, or dried crushed tea plant leaves to solvent extraction, supercritical extraction, or ultrasonic extraction. In this sense, the extract may be a solvent extract extracted with an extraction solvent, a supercritical extract, i.e., an extract obtained through supercritical extraction using a supercritical fluid, or an ultrasonic extract.

The solvent extract may be prepared according to conventional methods known in the art, using conventional solvents, specifically extraction solvents, under conventional conditions of temperature and pressure.

The extraction solvent may be any one selected from the group consisting of water, an organic solvent, or a mixture thereof that can be used for natural product extraction, preferably water, an alcohol having 1 to 5 carbon atoms, and a mixture thereof, more preferably water. The organic solvent may be a linear or branched alcohol having 1 to 5 carbon atoms such as methanol or ethanol, a polar solvent such as ethyl acetate or acetone and a non-polar solvent such as hexane or dichloromethane, a neutral solvent such as a ketone having 3 to 5 carbon atoms, or a mixture thereof, preferably a 30 to 90% aqueous solution of a linear or branched alcohol having 1 to 5 carbon atoms such as ethanol, or a 30 to 90% aqueous solution of a ketone having 3 to 5 carbon atoms, more preferably a 50 to 80% aqueous solution of ethanol or a 50 to 80% aqueous solution of acetone, and even more preferably a 60 to 75% aqueous solution of ethanol.

The extraction solvent may be replaced with other conventional solvents known to those skilled in the art that can exhibit substantially the same effect.

The supercritical extract may be extracted by supercritical extraction or supercritical fluid extraction, which uses carbon dioxide as a fluid and is performed under supercritical conditions of reduced pressure and high temperature. In general, a supercritical fluid has the properties of a liquid and a gas when a gas reaches its critical point under high temperature and pressure conditions, and has a polarity similar to that of a non-polar solvent chemically, so it is effective for extracting fat-soluble substances. Specifically, the carbon dioxide becomes a supercritical fluid that has the properties of both a liquid and a gas through a process in which the pressure and temperature reach the critical point by the operation of a supercritical fluid device, and as a result, the solubility of fat-soluble solutes increases. Therefore, when the supercritical carbon dioxide passes through an extraction vessel containing a certain amount of sample, the fat-soluble substances contained in the sample are extracted by the supercritical carbon dioxide, and after the fat-soluble substances have been extracted, if supercritical carbon dioxide containing a small amount of co-solvent is passed through the sample remaining in the extraction vessel again, components that could not be extracted with pure supercritical carbon dioxide alone can be extracted and come out.

In view of this, the supercritical fluid used in the supercritical extraction method may be supercritical carbon dioxide or a mixed fluid in which carbon dioxide is further mixed with a co-solvent. The co-solvent may be any one selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether, and mixtures thereof.

The carbon dioxide used in the extraction process volatilizes into the air at room temperature, so the extract obtained by the above method can be used as a cosmetic composition, and in the case of a co-solvent, it can be removed using a reduced pressure evaporator.

The ultrasonic extract means that it is extracted by ultrasonic extraction method. The ultrasonic extraction method is an extraction method that uses energy generated by ultrasonic vibration. When the ultrasonic waves are transmitted to a sample in an extraction solvent, the temperature rises due to the ultrasonic waves, and the high local temperature increases the kinetic energy of the reactant particles located around it, so that sufficient energy required for the reaction is obtained. The impact effect of the ultrasonic energy induces high pressure, which enhances the mixing effect of the substances contained in the sample and the extraction solvent, thereby increasing the extraction efficiency.

The extraction solvent that can be used in the ultrasonic extraction method may be any one selected from the group consisting of chloroform, ethanol, methanol, water, ethyl acetate, hexane, diethyl ether, and mixtures thereof.

The solvent extraction method can be cold extraction, hot extraction, pressure extraction or reflux extraction.

After the extraction process, the extract may be concentrated or the solvent may be removed by performing a vacuum filtration process, or by additional concentration and/or freeze-drying. Therefore, the tea leaf extract in the present invention is used to include a dried extract obtained by drying in a conventional manner, a concentrate obtained by concentrating in a conventional manner, and a dilution of the extract, the dried product, or the concentrate. The obtained tea leaf extract may be stored in a deep freezer until it is used.

The oral composition for improving bad breath may further contain one or more additional components selected from the group consisting of polyols, polymers, solubilizers, antibacterial components, active components for removing bad breath, components for preventing gum disease, pH regulators, preservatives, antiseptics, flavoring agents, sweeteners, flavorings, dyes, and solvents.

In addition to the active ingredients, the oral composition for improving bad breath of the present invention can easily be added by a person skilled in the art with auxiliary ingredients that are commonly used in the oral compositions of the present invention.

The solubilizer refers to a surfactant used to solubilize substances that are not soluble in water, and may be, for example, an anionic surfactant, an amphoteric surfactant, or a nonionic surfactant, and may be, for example, an anionic surfactant such as an amide-based nonionic surfactant, sodium lauryl sulfate, a polyoxyethylene-polyoxypropylene copolymer (poloxamer), polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, or a taurate-based anionic surfactant or a betaine-based amphoteric surfactant.

The antibacterial component is a substance having antibacterial activity, and refers to a component having antibacterial activity against bacteria causing dental caries, bacteria causing periodontitis, or bacteria causing gingivitis. Examples of the extracts include, but are not limited to, sodium chloride that can exert a strong osmotic effect, natural extracts that have antibacterial activity against the above-mentioned bacteria causing dental caries, bacteria causing periodontitis, and bacteria causing gingivitis, examples of which include grapefruit seed extract, propolis extract, dandelion extract, concentrated bellflower extract, concentrated Phellodendron Bark extract, peppermint extract, pine needle extract, willow extract, seaweed extract, and components derived from natural extracts, examples of which include triclosan, curcumazan triza oil, cetyl pyridinium chloride, benzethonium chloride, benzalconium chloride, cetyl pyridinium chloride, and chlorhexidine gluconate. The antibacterial component may be contained in an amount of 0.01 to 10 parts by weight or 0.05 to 5 parts by weight per 100 parts by weight of the oral composition for improving bad breath, but is not limited thereto.

The active ingredient for removing bad breath may be a nonfermented sugar alcohol, a natural extract, an inorganic compound, an enzyme, an enzyme inhibitor, etc.

The non-fermentable sugar alcohol may be xylitol, sorbitol, erythritol, mannitol, maltitol, lactitol, palatinitol, palatinose, oligosaccharides, etc., the natural extract may be persimmon leaf extract, sanguinaria extract, peony bark extract, etc., and the inorganic compounds may be zinc chloride, copper gluconate, sodium pyrophosphate, etc. sodium pyrophosphate (TSPP), sodium acid pyrophosphate (SAPP), sodium hexametaphosphate (SHMP), sodium tripolyphosphate (STP), sodium potassium tripolyphosphate (SKTP), potassium pyrophosphate (TKPP), sodium acid metapolyphosphate, sodium acid polyphosphate (acid The enzyme may be dextranase, glucose oxidase, glucose peroxidase, lactoperoxidase, glucanase, protease, or lysozyme, and the enzyme inhibitor may be an inhibitor of an enzyme involved in the generation of oral halitosis, such as a peptide inhibitor or a collagenase inhibitor.

The above-mentioned breath odor removing active ingredients can be used alone or in combination of two or more.

The active ingredient for removing bad breath can be added at a concentration of 0.001 to 30% by weight or 0.01 to 10% by weight based on the weight of the oral composition for improving bad breath.

The gum disease prevention ingredient may be sodium chloride, tocopherol acetate, pyridoxine hydrochloride, allantoin, aluminum chlorohydrate allantoin, tranexamic acid, or aminocaproic acid.

The gum disease prevention ingredients can be used alone or in combination of two or more types.

The gum disease prevention ingredient can be added at a concentration of 0.001 to 30% by weight or 0.01 to 10% by weight based on the weight of the oral composition for improving bad breath.

The pH regulator refers to a substance that regulates pH or buffers pH changes for a pH-sensitive active ingredient, such as an antibacterial ingredient or a medicinal substance. The pH regulator may be sodium phosphate, disodium phosphate, trisodium phosphate, sodium citrate, sodium bicarbonate, sodium pyrophosphate, citric acid, malic acid, succinic acid, tartaric acid, formic acid, or lactic acid.

The pH adjusters can be used alone or in combination of two or more.

The pH adjuster can be added at a concentration of 0.001 to 30% by weight or 0.01 to 10% by weight based on the weight of the oral composition for improving bad breath.

The flavoring agent is an ingredient added for the purpose of giving a fresh feeling to the oral composition for improving bad breath, suppressing unclean odors, and improving usability and palatability. Examples include ginseng extract, propolis extract, mandarin orange oil, L-menthol, cool mint scent (HF-61399), mint-scented flavoring agent, or compound flavor (apple scent, HF-30630), etc.

The sweetener is an ingredient added for the purpose of imparting a certain degree of sweetness to the oral composition for improving bad breath and imparting a refreshing feeling, etc., and examples thereof may be saccharin sodium, saccharin sodium hydrate, aspartame, stevioside, steviol glycoside, xylitol, D-xylose, glycyrrhizic acid, chamomile extract, sage extract, or propolis extract.

The flavoring is an edible flavoring, examples of which include menthol, anethole, eugenol, limonene, ocimene, n-dodecyl alcohol, citronellol, α-terpineol, methyl salicylate, methyl acetate, citronellyl acetate, cineol, linalool, and ethyl linalool. Linalool), vanillin, thymol, spearmint oil, sage oil, rosemary oil, cinnamon oil, peppermint oil, eucalyptol oil or fruit extracts.

The dyes used are mainly food dyes.

The solvent may be, for example, water or an organic solvent, preferably water or an aqueous ethanol solution, and more preferably water.

In addition, the oral composition for improving bad breath according to one embodiment of the present invention may also contain other additives that can be blended into the oral composition for improving bad breath.

The oral composition may be, but is not limited to, a mouthwash or gargle, and may be prepared in a liquid form to facilitate gargling.

[Production example: Production of active ingredients for oral compositions for improving bad breath]

To produce an oral composition for improving bad breath, the active ingredients chitosan and green tea extract were prepared.

First, high molecular weight chitosan was dissolved in 0.5% acetic acid, and the acetic acid chitosan solution was reacted with a chitosan decomposition enzyme, adjusting the decomposition time to obtain an appropriate molecular weight. The chitosan solutions treated with the enzyme for each time were then measured for average molecular weight using GPC, and chitosan with weight average molecular weights (MW) of 108,210 Da, 55,130 Da, 11,170 Da, 5,208 Da, and 815 Da were obtained.

In addition, because the palatability of the green tea extract is affected by the astringency of catechin, we purchased and used a powder of green tea extract with a catechin content of 20%.

The purchased green tea extract was added to the mouthwash, and the amount of green tea extract added was adjusted so that the concentrations of green tea extract were 0.1% (w/w, catechin content 0.02% w/w), 0.3% (w/w, catechin content 0.06% w/w), 0.5% (w/w, catechin content 0.1% w/w), and 1% (w/w, catechin content 0.2% w/w).

The following describes in detail the embodiments of the present invention so that those having ordinary skill in the art to which the present invention pertains can easily implement the present invention. However, the present invention can be embodied in various different forms, and the embodiments described herein are intended to illustrate the present invention, and the present invention is not limited to the following embodiments.

Each experiment was repeated three or more times, and the data obtained from each experiment was expressed as the mean and mean deviation (Mean ± SEM) using SPSS/WIN 12.0 version statistical program (Statistical Package for social Science, IBM, USA). To verify the significance between groups, one-way ANOVA was used, and the post-hoc test was performed using Duncan's multiple range test to verify the significance between each experimental group at the P < 0.05 level.

[Experimental example: Confirming the effectiveness of active ingredients in improving bad breath]

Experimental example 1-1. Confirmation of the amount of active ingredient added based on preference

For oral compositions, the effect of removing bad breath is important, but since various products can be selected based on the user's preferences and tastes, we first checked the content of the active ingredient by adding the degree of preference based on the amount of active ingredient added.

The oral composition used in the experiment was prepared by using purified water as a solvent in addition to the active ingredients, and further adding menthol (0.05% (w/w)), xylitol (3% (w/w)), and polyoxyethylene hydrogenated castor oil (0.05% (w/w)) in addition to green tea extract (Table 1) and chitosan (Table 2). The chitosan used had an average molecular weight of 11,170 Da.

To investigate the preference for the amount of active ingredient added to the prepared oral compositions, 10 adult men and women who were free of oral diseases or other illnesses that could affect the experiment and had undergone training based on sensory testing were selected. They used each oral composition and measured the overall preference using a 5-point scale. 5 points means the best, and 1 point means that the astringency was very strong and very poor. The measurement results are shown in Table 3 below.

As shown in Table 3, it was confirmed that when the content of green tea extract and chitosan increases, the astringency becomes stronger and the overall preference decreases, and in particular, when the content exceeds a certain level, the preference decreases significantly due to the strong astringency. Based on the results, it was determined that when green tea extract is added at 1.5% or more (0.3% (w/w) based on the catechin content), it is difficult to use due to the very strong astringency, and when green tea extract is added at 1.0% or less (0.2% (w/w) based on the catechin content), a certain level of preference can be secured, and it is evaluated that green tea extract should be used at 1.0% or less.

In the case of chitosan, when added at 0.15% or more, it was judged that it would be difficult to use due to its very strong astringent taste, and even when added at 0.1% or more, it was judged that there would be limitations to its sale and use as a product. However, when chitosan is added at 0.05% or less, a certain level of palatability can be secured, and it was assessed that chitosan should be used at 0.05% or less.

Experimental Example 1-2. Confirmation of the bad breath control effect by chitosan molecular weight

Based on the amount added confirmed in Experimental Example 1-1, the bad breath control effect of each active ingredient was confirmed. First, the bad breath control effect according to the molecular weight of chitosan was confirmed using the oral composition in Table 4 below.

In order to confirm the oral odor control effect of the prepared oral composition, 10 adult males and females without oral diseases or other diseases that may affect the experiment were selected, and the presence or absence of changes in oral odor was confirmed. Specifically, the oral odor value was measured using an oral odor meter (Oral Chroma CHM-2, Korea) using the substances causing oral odor (hydrogen sulfide (H _{2 S)} , methyl mercaptan (CH ₃ SH), and dimethyl sulfide ((CH ₃ ) ₂ S) as shown in FIG. 1, every 2 hours, before using the oral composition and one hour after gargling with 12 mL of the oral composition, and the results are shown in Tables 5 to 7 below. The reduction rate in Tables 5 to 7 below indicates the degree of reduction in the content of the oral odor components after use compared to before use.

As shown in Tables 5 to 7, when chitosan was used, the effect of removing bad breath was confirmed to be greater than that of the control group, but it was also confirmed that the effect of removing bad breath differed depending on the average molecular weight of chitosan. In particular, in the case of 11,170 Da, which was confirmed to be the most effective, the suppression effect was improved by about 175% or more compared to 815 Da, and by about 46% or more compared to 108,210 Da, and it was confirmed that it was improved by about 9 times compared to the control group. Therefore, it was confirmed that when using chitosan for mouthwash, it is most preferable to use chitosan with an average molecular weight of 11,170 Da.

Experimental Example 1-3. Confirmation of the effect of controlling bad breath depending on the amount of green tea extract

As in Experimental Example 1-2 above, the effect of controlling bad breath depending on the amount of green tea extract was confirmed using the oral composition in Table 8 below.

To confirm the bad breath control effect of the prepared oral composition, 10 adult men and women without oral diseases or other diseases that may affect the experiment were selected, and the presence or absence of changes in bad breath was confirmed as in Experimental Example 1-2, and the results are shown in Tables 9 to 11 below. The reduction rate in Tables 9 to 11 below indicates the degree to which the content of bad breath components was reduced after use compared to before use.

As shown in Tables 9 to 11, when green tea extract was used, the bad breath removing effect was improved compared to the control group, and the bad breath removing effect improved as the amount used increased, but the difference between the addition of 0.5% and 1.0% was limited, and it was confirmed that the bad breath removing effect was superior when 0.5% was added. In particular, unlike 0.5%, which was confirmed to be the most effective, it was confirmed that the preference also rapidly decreased at 1.0%, as shown in Experimental Example 1-1 and Table 3 above, and it was confirmed that the content of green tea extract contained in the oral composition was most preferably 0.5% (w/w).

Experimental Example 1-4. Confirmation of the bad breath control effect of green tea extract and chitosan molecular weight

Based on the results confirmed in Experimental Examples 1-2 and 1-3, it was confirmed that the combination of green tea extract and chitosan had an effect of controlling bad breath. The effect of controlling bad breath was confirmed using the oral composition in Table 12 below.

To confirm the bad breath control effect of the prepared oral composition, 10 adult men and women without any oral or other diseases that may affect the experiment were selected, and the presence or absence of changes in bad breath was confirmed as in Experimental Examples 1-2 and 1-3, and the results are shown in Tables 13 to 15 below. The reduction rate in Tables 13 to 15 below indicates the degree to which the content of bad breath components was reduced after use compared to before use.

As shown in Tables 13 to 15, when green tea extract and chitosan were used in combination, the effect of removing bad breath was generally significantly improved, but the tendency according to the molecular weight of chitosan was similar to that of Experimental Example 1-2 in which green tea extract was not used, and it was confirmed that green tea extract does not affect the effect of removing bad breath according to the molecular weight of chitosan. Specifically, in the case of 11,170 Da, which was confirmed to be the most effective, the suppression effect was improved by about 58% or more compared to 815 Da, and was improved by about 21% or more compared to 108,210 Da, and was improved by about 8 times compared to the control group. Therefore, it was confirmed that when using chitosan for mouthwash, it is most preferable to use chitosan with an average molecular weight of 11,170 Da, even when used in combination with green tea extract.

Experimental Example 1-5. Confirmation of the effect of green tea extract and chitosan in removing protein waste products

Based on the results confirmed in Experimental Examples 1-2 to 1-4, an oral composition containing 0.5% green tea extract and 0.05% chitosan with an average molecular weight of 11,170 Da was used to gargle for 30 seconds, and the effect of removing protein decomposition by-products, i.e., protein waste products, from the oral cavity was confirmed. To confirm the adsorption and discharge of protein decomposition by-products from the oral cavity, caramel coloring was dissolved in the mouthwash, and the adsorbed by-products were stained brown caramel coloring. When the mouthwash was spat out after gargling, the protein decomposition by-products were confirmed as brown by-products. The experimental results are shown in Figure 2.

As shown in Figure 2, when an oral composition containing 0.5% green tea extract and 0.05% chitosan with an average molecular weight of 11,170 Da was used for gargling for 30 seconds and then spat out, it was confirmed that significantly more protein degradation by-products were removed from the oral cavity than before use.

The above results confirm that the oral composition of the present invention can effectively control bad breath by removing protein degradation by-products in the oral cavity.

The present invention has been described above with reference to one embodiment, but this is merely an example, and a person having ordinary skill in the art will understand that various modifications and equivalent embodiments are possible.

The present invention uses the natural products chitosan and green tea extract as the main ingredients, minimizing the use of synthetic compounds that are harmful to the human body, making it not only safe, but also highly palatable and effective at eliminating bad breath, without causing inconvenience in use such as astringency, and improving the consumer's usability and satisfaction, so it can be used as an oral composition for improving bad breath in a variety of applications.

Claims (4)

An oral composition for improving bad breath, comprising chitosan and green tea extract as active ingredients,
The chitosan has a weight average molecular weight (Mw) of 10,000 Da to 57,000 Da;
The oral composition for improving bad breath has a chitosan content of 0.01 to 0.05 parts by weight based on 100 parts by weight of the oral composition for improving bad breath,
The green tea extract contains catechins,
The oral composition for improving bad breath, characterized in that the content of the green tea extract is 0.1 to 0.2 based on the content of catechin contained in the green tea extract, based on 100 parts by weight of the oral composition for improving bad breath . The oral composition for improving bad breath according to claim 1, wherein the chitosan has a weight average molecular weight (Mw) of 10,000 Da to 13,000 Da. The oral composition for improving bad breath according to claim 2, wherein the chitosan has a weight average molecular weight (Mw) of 11,150 Da to 11,200 Da. The oral composition for improving bad breath according to any one of claims 1 to 3, further comprising one or more additional components selected from the group consisting of polyols, polymers, bactericides, solubilizers, antibacterial components, active components for removing bad breath, components for preventing gum disease, pH adjusting agents, preservatives, antiseptics, flavoring agents, sweeteners, flavorings, colorants , and solvents.