JP7552066B2 - Film and transfer sheet for attaching to biological tissue - Google Patents

Description

The present invention relates to a film and a transfer sheet for application to biological tissue.

Extremely thin films with a thickness of several nm to several μm have high flexibility and therefore show high conformity to the surface shape of biological organs. Therefore, since the above-mentioned film adheres to the surface of biological organs without the use of adhesives or pressure-sensitive adhesives, attempts have been made to use the film by attaching it to an adherend such as an organ or skin. For example, Patent Document 1 describes the use of the above-mentioned film for cosmetic purposes. In cosmetic applications, the above-mentioned film is attached to the skin to assist with skin care and makeup. Patent Document 2 describes the use of the above-mentioned film for medical purposes. In medical applications, the above-mentioned film is attached to organs or skin for the purpose of closing incisions made during surgical operations, protecting areas with burns or wounds, and stopping bleeding.

International Publication No. 2014/058060 International Publication No. 2016/204266

In cosmetic and medical applications, it is desired that the film attached to the adherend can suppress the permeation of moisture, so that the film can form a closed state on the surface of the adherend.For example, in cosmetic applications, the closed state can be formed, so that moisture is retained on the surface of the skin and the stratum corneum moisture content is increased, resulting in a moisturizing effect.In addition, in medical applications, the closed state can be formed, so that a state suitable for moist therapy that improves the wound healing environment by wetting, and for the closed occlusion method that enhances the penetration of drugs into the skin can be created.
However, conventional films, which have been made thinner to improve their ability to conform to the surface shape of an adherend, are easily permeable to moisture due to their thinness, and therefore it is difficult to say that they have a sufficient moisture retention function.
An object of the present invention is to provide a film for application to biological tissue and a transfer sheet which have a high moisture retention effect and which are capable of conforming to the surface shape of an adherend.

In order to solve the above problems, one aspect of the present invention provides a biological tissue patch film including a moisture retaining layer, the moisture retaining layer including a water retaining component, a moisture absorbing component, and a polymeric material, and having a mass per unit area in the range of 0.1 g/ ^m2 to 6.0 g/ ^m2 . According to the above configuration, the moisture absorbing component can absorb water vapor generated from the skin as well as water vapor in the outside air. The moisture retaining component receives the absorbed moisture released by the moisture absorbing component, thereby maintaining moisture within the film and improving the moisture retention of the skin.

In the above configuration, the moisturizing layer may include a water retention layer and a water absorption layer in contact with the water retention layer, the water retention layer may include the water retention component and a first polymer material, and the water absorption layer may include the moisture absorption component and a second polymer material. According to the above configuration, the water absorption layer can absorb water vapor generated from the skin as well as water vapor in the outside air. The water retention layer can retain moisture within the film by receiving the absorbed moisture released by the water absorption layer, thereby improving the moisturizing properties of the skin.

In the above configuration, the moisture retention rate of the water retention component may be 35% or more, and the moisture absorption rate of the moisture absorption component may be 50% or more. According to the above configuration, the water retention component and the moisture absorption component can fully exert their functions to obtain a moisturizing effect.
In the above-mentioned configuration, the total content of the water-retaining component and the hygroscopic component in the moisturizing layer may be in the range of 1% by mass to 80% by mass. According to the above-mentioned configuration, the hygroscopic component effectively absorbs water, and the moisture-retaining function of the moisturizing component functions, thereby obtaining a good moisturizing effect.

In the above configuration, a protective layer may be provided on one or both sides of the film. According to the above configuration, the strength of the biological tissue patch film is improved, and damage when it is attached to the adherend can be prevented. In addition, by attaching the protective layer side to the adherend surface, excessive wetting of the adherend, which causes redness and itching, can be prevented.
In the above-mentioned configuration, the blockage rate in the moisturizing layer may be 10% or less. According to the above-mentioned configuration, water vapor can easily permeate through the biological tissue patch film, and the moisturizing component and the hygroscopic component can efficiently absorb and retain water vapor.

In order to solve the above problems, the transfer sheet of the present invention is characterized in that it has a support substrate on one side of the outermost layer of the film for application to biological tissue described above. According to the above configuration, since the film for application to biological tissue is supported by the support substrate, deformation of the film for application to biological tissue is suppressed, and it is also considered that the transfer sheet becomes easier to handle.

According to the present invention, a film that conforms to the surface shape of an adherend can moisten the adherend and enhance its moisturizing function.

FIG. 1 is a diagram showing a cross-sectional structure of a first form of a film to be attached to biological tissue according to one embodiment. FIG. 2 is a diagram showing a cross-sectional structure of a second form of the film for application to biological tissue according to one embodiment. FIG. 13 is a diagram showing a cross-sectional structure of a third form of the biological tissue patch film of one embodiment. FIG. 13 is a diagram showing a cross-sectional structure of a fourth form of the biological tissue patch film according to one embodiment. FIG. 2 is a diagram showing a cross-sectional structure of a first form of a biological tissue patch film transfer sheet. FIG. 13 is a diagram showing a cross-sectional structure of a second form of a biological tissue patch film transfer sheet. 1A to 1C are diagrams showing a manufacturing process of a film for application to biological tissue according to one embodiment. 1A to 1C are diagrams showing a manufacturing process of a film for application to biological tissue according to one embodiment. FIG. 2 is a diagram showing a transfer method for a film to be attached to biological tissue according to one embodiment, illustrating a step of applying a liquid material to an adherend. FIG. 2 is a diagram showing a transfer method for a film to be applied to biological tissue according to one embodiment, illustrating a step of applying a transfer sheet. FIG. 2 is a diagram showing a transfer method for a film to be applied to biological tissue according to one embodiment, illustrating a step of peeling off a supporting substrate.

An embodiment of the film for application to biological tissue and the transfer sheet of this embodiment will be described with reference to the drawings. The film for application to biological tissue of this embodiment is a film for application to biological tissue that includes a moisture-retaining layer, and the moisture-retaining layer includes a water-retaining component, a moisture-absorbing component, and a polymeric material. The adherend to which the film for application to biological tissue of this embodiment is applied is biological tissue, such as skin or an organ. The film for application to biological tissue of this embodiment may be particularly suitable for application to skin.

The film for application to biological tissue of this embodiment is not particularly limited in its application, and may be used for cosmetic applications or medical applications. When used for cosmetic applications, the film for application to biological tissue is applied to the skin to assist with skin care and makeup. When used for medical applications, the film for application to biological tissue is applied to the skin or organs to protect wounds or areas to which medicines are applied.

[Configuration of film for application to biological tissue]
As shown in Fig. 1, the biological tissue patch film 10A of the first embodiment is a film made of a polymeric material, which may be an organic polymeric material, and a film made of an organic polymeric material is called an organic polymeric material film. The film is provided with a moisture retaining layer 13 containing a water retaining component 11 and a moisture absorbing component 12.
With the configuration of the biological tissue patch film 10A, the moisture absorbing component 12 absorbs water vapor generated from the skin and water vapor in the outside air, and the moisture is received by the moisture retaining component 11, thereby maintaining moisture.

As shown in FIG. 2, the second form of biological tissue patch film 10B has a moisture retaining layer 13 which is a laminate of a moisture retaining layer 14 containing a moisture retaining component 11 in an organic polymer material film and a moisture absorbing layer 15 containing a moisture absorbing component 12 in an organic polymer material film.
With the configuration of the biological tissue patch film 10B, the moisture absorbing component 12 in the moisture absorbing layer 15 absorbs water vapor generated from the skin as well as water vapor in the outside air, and the moisture is received by the moisture retaining component 11 in the moisture retaining layer 14, which absorbs and retains the moisture, thereby maintaining moisture.
As shown in FIG. 3, the third form of biological tissue patch film 10C has a protective layer 16 made of an organic polymer material laminated on the outermost layer on the side opposite to the side of the biological tissue patch film 10A or the biological tissue patch film 10B that is to be attached to the adherend.

As shown in FIG. 4, the biological tissue patch film 10D of the fourth embodiment has protective layers 16 made of organic polymer material laminated on both outermost layers of the biological tissue patch film 10A or the biological tissue patch film 10B.
The biological tissue patch film 10C and the biological tissue patch film 10D, which have a protective layer 16 laminated on one or both sides of the biological tissue patch film 10A or the biological tissue patch film 10B, have a stronger film and are easier to handle than the biological tissue patch film 10A or 10B which does not have the protective layer 16 laminated thereon, and also have the effect of suppressing the outflow of the water-retaining component 11 and the moisture-absorbing component 12 due to excess moisture.

These biological tissue patch films 10 have a mass per unit area in the range of 0.1 g/m2 ^{or more} and 6.0 g/m2 or ^less . If the mass per unit area of the biological tissue patch film 10 is within the above range, the film can be well conformed to the surface shape of the adherend, and the film alone can exhibit adhesiveness to the adherend. If the mass per unit area is less than 0.1 g/ ^m2 , the film contains too little moisturizing ingredient and cannot exhibit sufficient effect, and if the mass per unit area is more than 6.0 g/ ^m2 , the nanofilm cannot exhibit adhesiveness to the adherend.

The mass per unit area is the mass of the biological tissue patch film 10 converted per part having an area of ^{1 m2} in a plan view. The mass per unit area can be calculated, for example, from the average value of masses measured in a plurality of measurement regions, or by multiplying the average thickness of the biological tissue patch film 10 by the density. The density of the biological tissue patch film 10 is, for example, in the range of 1 g/ ^cm3 to 3 g/ ^cm3 .

In the biological tissue patch film 10, the blocking rate may be 10% or less. If the blocking rate is within the above range, the permeation of water vapor into the organic polymer material film is not hindered, and the moisture absorbing component 12 efficiently absorbs the water vapor in the skin and in the outside air, and the moisture retaining component 11 can retain it, and the moisturizing effect is not impaired. If the blocking rate of the biological tissue patch film 10 is greater than 10%, the permeation of water vapor into the organic polymer material film is hindered, and the moisture absorbing component 12 cannot efficiently absorb the water vapor in the skin and in the outside air, and the moisture retaining component 11 cannot retain it, and the moisturizing effect is impaired.

The above-mentioned occlusion rate is the ratio at which the amount of evaporation of water having a temperature equal to human body temperature at the location where the film 10 for application to biological tissue is placed is suppressed relative to the amount of evaporation when the film 10 for application to biological tissue is not placed. The occlusion rate may be expressed as Br and may be measured by the following method.

1) Warm water from a water bath maintained at 37° C. is circulated inside a tight box. A glass sample bottle is placed in the tight box, and 37° C. warm water is poured into the glass sample bottle up to a position 4 cm vertically away from the opening of the glass sample bottle.
2) Place a plastic plate with a hole of 9 mm diameter on the opening of the glass sample bottle in 1) above, cover the hole with a PTFE membrane filter (Merck Millipore, pore size: 10 μm, diameter: 25 mm, plain white), and leave for 5 minutes.
3) Using a portable moisture evaporation meter (Delfin Technologies: VapoMeter) and a 9 mm Franz cell adapter, the amount of moisture evaporation at the position of the hole in the plastic plate after the operation in 2) above is measured on the membrane filter, and the measured value is regarded as the initial value.

4) Place the transfer sheet on the membrane filter in 2) above so that the skin contact surface of the film 10 for application to biological tissue is in contact with the membrane filter, and press the transfer sheet with fingers to attach the transfer sheet to the membrane filter. Then, peel off the support substrate from the film 10 for application to biological tissue and leave it for 5 minutes.
5) Using the portable moisture evaporation meter, the amount of moisture evaporation at the position of the hole in the plastic plate after the operation of 4) is measured on the film 10 for application to biological tissue in the same manner as in 3) above, and the measured value is taken as a sample value. The occlusion rate is calculated by the formula: occlusion rate = (initial value - sample value) / initial value x 100.

The detailed configuration of each layer constituting the film 10 for application to biological tissue will be described below.
The moisture-retaining component 11 contained in the moisturizing layer 13 is a material with a moisture retention rate of 35% or more, and is not particularly limited as long as it is within the above-mentioned moisture retention rate range, but mucopolysaccharides such as hyaluronic acid, sodium hyaluronate, chondroitin, chondroitin sulfate, heparin, heparin sulfate, carrageenan, sacran, dermatan sulfate, etc., water-absorbent polymers such as polyacrylic acid, sodium polyacrylate, polyacrylic acid copolymer, maleic acid copolymer, starch, carboxymethyl cellulose, polyvinyl alcohol, etc. are used, and sodium hyaluronate and sodium polyacrylate are particularly preferred. If the moisture retention rate is a component of the above-mentioned moisture retention rate, the moisture-retaining component 11 can fully exert its function and obtain a moisturizing effect.

The moisture retention rate is the degree to which a material retains moisture, and may be, for example, the moisture remaining rate calculated using the following formula after absorbing 20 μL of an aqueous solution with a concentration of 1% by weight of the material into a Φ40 mm filter paper and leaving it for 15 minutes in an environment of 20° C. and 65% humidity.
Moisture retention rate = 1 - (total amount of absorbed moisture / amount of moisture after 15 minutes)

The moisture absorbing component 12 contained in the moisturizing layer 13 is a material with a moisture absorption rate of 50% or more, and although not particularly limited, polyhydric alcohols such as glycerin, diglycerin, hexylene glycol, dipropylene glycol, ethylene glycol, propylene glycol, polyethylene glycol, polypropylene glycol, triethylene glycol, polyglycerin, and 1.3 butylene glycol, and inorganic salts such as sodium chloride, lithium chloride, calcium chloride, magnesium chloride, sodium sulfate, and magnesium sulfate may be used, with glycerin, diglycerin, and polyglycerin being particularly preferred. With the above moisture absorbing component 12, the water retention component can fully exert its function and a moisturizing effect can be obtained.

The moisture absorption rate is the degree to which a material absorbs water vapor in the atmosphere, and may be calculated from the following formula for 0.2 g of material after storage at a temperature of 40° C. and a humidity of 90% for 1 hour.
Moisture absorption = (weight of material after moisture absorption - initial weight of material) / initial weight of material

The organic polymer material film carrying the water-retaining component 11 and the moisture-absorbing component 12 or the protective layer 16 laminated on the moisturizing layer 13 is not particularly limited. Examples of the organic polymer material include ester resins such as polylactic acid, polyglycolic acid, and polycaprolactone, and copolymer resins thereof; acrylic resins, silicones, and copolymer resins thereof, which are resins used as film-forming agents in cosmetics; cellulose derivatives such as cellulose acetate, cellulose acetate propionate, and cellulose acetate butyrate; and resins with a strong track record of use in the medical field, such as polyethylene, polypropylene, polycarbonate, cycloolefin polymers, cycloolefin copolymers, styrene butadiene elastomers, polyvinylidene fluoride, polyimide, and polyvinyl alcohol. The organic polymer material may be hydrophobic or hydrophilic, or may contain multiple types of materials. The organic polymer material of the moisturizing layer 13 and the organic polymer material of the protective layer 16 may be the same or different. Furthermore, when protective layers 16 are provided on both sides of the moisture-retaining layer 13, the organic polymer materials used for the protective layers 16 on both sides may be the same or different. In particular, hydrophilic polymers that are highly compatible with the moisture-retaining component 11 and the moisture-absorbing component 12 are preferred.

The ratio of the water-retaining component 11 and the moisture-absorbing component 12 in the moisture-retaining layer 13 may be 1% to 80% by weight, with the combined ratio of the water-retaining component 11 and the moisture-absorbing component 12 being preferably in the range of 30% to 60% by weight and 5% to 20% by weight. With the above content, when the biological tissue patch film 10 is applied to the skin, the moisture-absorbing layer 15 can absorb a sufficient amount of water vapor generated from the skin as well as water vapor in the outside air. The moisture-absorbing layer 15 releases the absorbed moisture and the moisture-retaining layer 14 receives it, thereby maintaining moisture within the film and improving the moisture-retaining properties of the skin. If the moisture-retaining component 11 is 1% or less, the moisture-retaining function is not sufficient, and similarly, if the moisture-absorbing component 12 is 1% or less, the moisture-absorbing function is not sufficient and the moisturizing effect is not obtained. If the content of the moisture-retaining component 11, the moisture-absorbing component 12, or the combined content of the moisture-retaining component 11 and the moisture-absorbing component 12 is 80% or more, the amount of moisture-retaining component and the moisture-absorbing component is too high, and the strength and flexibility of the film are insufficient, which may result in poor operability of the biological tissue patch film 10.

With these configurations, the biological tissue patch film 10 has adhesiveness to biological tissue such as skin, and the moisture absorbing component 12 adsorbs water vapor released from biological tissue or water vapor in the atmosphere. The moisture absorbing component 12 also has a moisture retention function, but its retention capacity is low, and the moisture retention component 11 also has a moisture absorption effect, but the moisture retention function and moisture absorption function are in a trade-off relationship, so the moisture absorption function is low. By mixing the moisture retention component 11 and the moisture absorbing component 12, the water absorbed by the moisture absorbing component 12 creates an environment with a lot of water vapor around the moisture retention component 11, which increases the adsorption efficiency of the moisture retention component 11 and enables it to efficiently absorb and retain moisture.

[Configuration of transfer sheet]
Fig. 5 is a diagram showing a cross-sectional structure of a first embodiment of a biological tissue patch film transfer sheet. Fig. 6 is a diagram showing a cross-sectional structure of a second embodiment of a biological tissue patch film transfer sheet. The transfer sheet 20 is used when attaching the biological tissue patch film 10 to an adherend, and includes the biological tissue patch film 10 and a supporting substrate 21 that supports the biological tissue patch film 10. The supporting substrate 21 may be provided on one side of the biological tissue patch film 10 as shown in Fig. 5, or on both sides of the biological tissue patch film 10 as shown in Fig. 6.

The supporting substrate 21 has a function of suppressing deformation of the biological tissue patch film 10 when the biological tissue patch film 10 is moved onto an adherend during storage of the biological tissue patch film 10 or when the biological tissue patch film 10 is used. Being supported by the supporting substrate 21 makes the biological tissue patch film 10 easier to handle. When the supporting substrate 21 is provided on both sides, the biological tissue patch film 10 can be attached to the adherend by first peeling off the side to be adhered.

The support substrate 21 is preferably a porous substrate. The porous substrate is a substrate having a large number of minute gaps inside, and can allow liquid to permeate or pass through. Examples of porous substrates that can be used as the support substrate 21 include sheets made of fiber materials such as nonwoven fabric, paper, knitted fabric, and woven fabric, and resin sheets having a mesh-like structure that includes gaps. Among these substrates, nonwoven fabric is preferably used. Fibers constituting the nonwoven fabric include natural fibers such as cotton, hemp, wool, and pulp, semi-synthetic fibers such as rayon, and synthetic fibers such as polyvinyl alcohol and polyacrylic acid. Among the above fibers, natural fibers, particularly pulp, are preferably used. The nonwoven fabric may be composed of one type of fiber, or may be composed of two or more types of fibers.
The support substrate 21 is not limited to a porous substrate, and may be a substrate such as a resin sheet or metal foil having no internal gaps. In addition, a substrate having releasability, such as a resin sheet having a surface made of silicone or fluororesin, may be used as the support substrate 21.

[Method of manufacturing biological tissue patch film and transfer sheet]
The following describes a method for manufacturing the biological tissue patch film 10 and the transfer sheet 20. First, the following describes a method for manufacturing a mixture used to form the moisture-retaining layer 13 of the biological tissue patch film 10. The moisture-retaining layer 13 is formed by mixing an organic polymer material, which is a material for an organic polymer material film containing a moisture-retaining component 11 and a moisture-absorbing component 12, with the material for the moisture-retaining component 11 and the material for the moisture-absorbing component 12 described above to prepare a mixture, and then applying and drying the mixture to a film-forming substrate 30, which is a releasable substrate.

The mixed liquid is preferably produced by preparing a dispersion in which the water-retentive component 11 and the moisture-absorbing component 12 are dispersed in an appropriate dispersion medium, and then mixing the dispersion with a solution in which an organic polymer material is dissolved in an appropriate solvent. By adjusting the mixing ratio between the dispersion and the organic polymer material solution, a mixed liquid containing the water-retentive component 11, the moisture-absorbing component 12, and the organic polymer material in a desired ratio can be obtained.
The dispersion medium and the solvent preferably have compatibility with the water-retaining component 11, the moisture-absorbing component 12, and the organic polymer material. Each of the dispersion medium and the solvent may be composed of a single medium or may be a mixed solution of a plurality of types of media.

A method for manufacturing the film 10 for application to biological tissue will be described. As shown in FIG. 7, first, a moisture-retaining layer 13 is formed on a film-forming substrate 30. The moisture-retaining layer 13 is formed by mixing a water-retaining component 11, a moisture-absorbing component 12, and an organic polymer material in an appropriate solvent, applying the resulting solution to the upper surface of the film-forming substrate 30 to form a coating film, and then drying the coating film. The method for applying the solution is not particularly limited, and the solution may be applied by a known application method using, for example, a spin coater, a bar coater, a roll coater, a die coater, a gravure coater, or the like.

The film formation substrate 30 may be any substrate having releasability, and for example, a polyethylene terephthalate film or an olefin film having a surface made of silicone or fluorine resin may be suitably used.
The mixed liquid is applied to a film-formation substrate 30, which is a releasable substrate, and then dried to form a film 10A for application to biological tissue on the film-formation substrate 30. The film 10A for application to biological tissue can be obtained alone by peeling the film 10A for application to biological tissue from the film-formation substrate 30. For example, the film 10A for application to biological tissue can be peeled off from the film-formation substrate 30 by making a cut between the film-formation substrate 30 and the film 10A for application to biological tissue with a razor to create an opening for peeling the film 10A for application to biological tissue from the film-formation substrate 30.

As shown in FIG. 8, a protective layer 16 may be formed on the moisturizing layer 13 applied to the film-forming substrate 30 described above. The protective layer 16 may be formed by applying a mixed liquid in which an organic polymer material is mixed with a solvent to the upper surface of the moisturizing layer 13 to form a coating film, and then drying the coating film. The method for applying the mixed liquid is not particularly limited, and the mixed liquid may be applied by a known coating method using, for example, a spin coater, a bar coater, a roll coater, a die coater, a gravure coater, or the like.

This forms the film 10C for application to biological tissue, which is a laminate of the moisturizing layer 13 and the protective layer 16. By peeling the film 10C for application to biological tissue from the film-forming substrate 30, a single film 10C for application to biological tissue can be obtained. For example, the film 10C for application to biological tissue can be peeled off from the film-forming substrate 30 by making an incision with a razor between the film-forming substrate 30 and the film 10C for application to biological tissue to create an opening.

In addition, before or after peeling off the film 10 for application to biological tissue from the film-formation substrate 30, a support substrate 21 is laminated on the film 10 for application to biological tissue to form a transfer sheet 20. For example, the support substrate 21 is brought into contact with the upper surface of the film 10 for application to biological tissue on the film-formation substrate 30, and the film 10 for application to biological tissue is transferred from the film-formation substrate 30 to the support substrate 21 to form the transfer sheet 20. As a transfer method, a known transfer method such as a method using peeling by suction or a method using a sacrificial film may be used.
In addition, as long as it is possible to form a single-layer moisturizing layer 13, a laminated structure of the moisturizing layer 13 and the protective layer 16, and a laminated structure of the film 10 for application to biological tissue and the supporting substrate 21, the manufacturing method of the film 10 for application to biological tissue and the transfer sheet 20 may be different from the above-mentioned method.

[Method of applying a biological tissue patch film]
9, 10 and 11, a method for attaching the film 10 to an adherend, i.e., a method for transferring the film 10 to an adherend using a transfer sheet 20, will be described. In the following, as an example, a case where the film 10 to be attached to a skin as an adherend in cosmetic applications will be described.

As shown in Figure 9, first, liquid Lq, which may be water, skin lotion, lotion, beauty cream, or any other liquid, is applied to the skin Sk, and the area where the liquid Lq is applied is designated as the application area. Then, as shown in Figure 10, the film 10 for application to biological tissue is placed on the application area of the liquid Lq so that it is attached to the adherend, and the transfer sheet 20 is placed on the skin Sk. Note that in the case of a transfer sheet 20B structure in which the support substrate 21 is laminated on both sides, the support substrate 21 on the surface to be attached to the adherend is peeled off in advance.

Next, as shown in FIG. 11, the support substrate 21 is peeled off from the film 10 for application to biological tissue. This causes the film 10 for application to biological tissue to be transferred to the skin Sk. The film 10 for application to biological tissue, together with the liquid Lq, follows the surface shape of the skin Sk and adheres closely to the skin Sk. Note that as time passes, the volume of the liquid Lq located between the film 10 for application to biological tissue and the surface of the skin Sk decreases due to the liquid Lq being absorbed by the skin Sk, etc. As a result, it is believed that the adhesion between the film 10 for application to biological tissue and the skin Sk is improved.

In the film 10 for application to biological tissue of this embodiment, the moisturizing function is enhanced by the moisturizing layer 13, so in cosmetic applications, the moisturizing properties of the skin in the area where the film 10 for application to biological tissue is applied are improved. In medical applications, a moist environment is preferably created during wound healing, and when a drug is applied to the adherend, good penetration of the drug is expected to be obtained.

It is not essential to apply the liquid Lq to the adherend before applying the film 10 for application to biological tissue, and the film 10 for application to biological tissue may be applied to an unapplied adherend. On the other hand, the film 10 for application to biological tissue may be used to protect an application area of a cosmetic cream or a drug on the adherend. That is, after applying a cosmetic cream or a drug to the surface of the adherend, the film 10 for application to biological tissue is applied to the application area, thereby covering the application area with the film 10 for application to biological tissue. This makes it possible to prevent moisture and dirt from adhering to the application area, and therefore allows the cosmetic ingredients and medicinal ingredients to remain near the surface of the adherend for a long period of time.

[Example]
The above-mentioned biological tissue patch film 10 and transfer sheet 20 will be described using specific examples and comparative examples. The manufacturing methods of the examples and comparative examples will be described in detail below.
Example 1
To prepare the moisture-retaining layer 13, an aqueous solution was prepared with a total solid content of 3% by weight, a ratio of the moisture-retaining component 11 in the solid content of 40% by weight, a ratio of the moisture-absorbing component 12 of 10% by weight, and a ratio of the organic polymer material of 50% by weight. Sodium hyaluronate was used as the moisture-retaining component 11, and glycerin was used as the moisture-absorbing component 12. Polyvinyl alcohol (Mitsubishi Chemical, Gohsenol EG-40) was used as the organic polymer material. A biaxially oriented polypropylene film (Futamura Chemical: FOS#50) was used as the film-forming substrate 30, and the above solution was applied to the film-forming substrate 30 using a bar coating method to form a coating film. The coating film was dried at 90°C for 2 minutes to form a moisture-retaining layer 13 with a coating amount of about 0.8 g/ ^m2 .

Next, a solution in which cellulose acetate was dissolved in butyl acetate at a concentration of 10% by mass was applied onto the moisture-retaining layer 13 by a bar coating method to form a coating film. The coating film was dried at 90° C. for 2 minutes to form a protective layer 16 with a coating amount of about 0.2 g/ ^m2 .
As a result, the film 10 for application to biological tissue of Example 1, which is composed of the moisture retaining layer 13 and the protective layer 16, was obtained.
Next, a polyester nonwoven fabric was laminated as a support substrate 21 on the surface of the film 10 for application to biological tissue on the film-forming substrate 30, i.e., on the surface of the composite layer, and then the laminate of the film 10 for application to biological tissue and the support substrate 21 was peeled off from the film-forming substrate 30. In this way, the transfer sheet 20 of Example 1 was obtained.

Example 2
A film 10 for application to biological tissue and a transfer sheet 20 of Example 2 were obtained in the same manner as in Example 1, except that sodium polyacrylate was used as the water-retaining component 11.
Example 3
The film 10 for application to biological tissue and the transfer sheet 20 of Example 3 were obtained in the same manner as in Example 1, except that L-proline was used as the moisture absorbing component 12.
Example 4
A film 10 for application to biological tissue and a transfer sheet 20 of Example 4 were obtained in the same manner as in Example 1, except that sodium polyacrylate was used as the water-retaining component 11 and L-proline was used as the moisture-absorbing component 12.

Example 5
To prepare the water-retaining layer 14, an aqueous solution was prepared in which the total solid content was 3% by weight, the proportion of the water-retaining component 11 in the solid content was 40% by weight, and the proportion of the organic polymer material was 60% by weight. Sodium hyaluronate was used as the water-retaining component 11. Polyvinyl alcohol (Mitsubishi Chemical Co., Ltd., Gohsenol EG-40) was used as the organic polymer material. A biaxially oriented polypropylene film (Futamura Chemical Co., Ltd.: FOS#50) was used as the film-forming substrate 30, and the above solution was applied to the film-forming substrate 30 using a bar coating method to form a coating film. The coating film was dried at 90°C for 2 minutes to form a water-retaining layer 14 with a coating amount of about 0.4 g/ ^m2 .

To prepare the moisture absorbing layer 15, an aqueous solution was prepared with a total solid content of 3% by weight, a ratio of the moisture absorbing component 12 in the solid content of 10% by weight, and a ratio of the organic polymer material of 90% by weight. Glycerin was used as the moisture absorbing component 12. Polyvinyl alcohol (Gosenol EG-40, manufactured by Mitsubishi Chemical) was used as the organic polymer material. The solution was applied onto the moisture retaining layer 14 using a bar coating method to form a coating film. The coating film was dried at 90°C for 2 minutes to form the moisture absorbing layer 15 with a coating amount of approximately 0.4 g/ ^m2 , and the moisture retaining layer 13 consisting of the moisture retaining layer 14 and the moisture absorbing layer 15 was formed.

Next, a solution in which cellulose acetate was dissolved in butyl acetate at a concentration of 10% by mass was applied onto the moisture-retaining layer 13 by a bar coating method to form a coating film. The coating film was dried at 90° C. for 2 minutes to form a protective layer 16 with a coating amount of about 0.2 g/ ^m2 .
As a result, the film 10 for application to biological tissue of Example 5, which is composed of the moisture retaining layer 13 and the protective layer 16, was obtained.
Next, a polyester nonwoven fabric was laminated as a support substrate 21 on the surface of the film 10 for application to biological tissue on the film-forming substrate 30, i.e., on the surface of the composite layer, and then the laminate of the film 10 for application to biological tissue and the support substrate 21 was peeled off from the film-forming substrate 30. In this way, the transfer sheet 20 of Example 5 was obtained.

Example 6
The film 10 for application to biological tissue and the transfer sheet 20 of Example 6 were obtained in the same manner as in Example 5, except that sodium polyacrylate was used as the water-retaining component 11.
(Example 7)
The film 10 for application to biological tissue and the transfer sheet 20 of Example 7 were obtained in the same manner as in Example 5, except that L-proline was used as the moisture-absorbing component 12.
(Example 8)
The film 10 for application to biological tissue and the transfer sheet 20 of Example 8 were obtained in the same manner as in Example 5, except that sodium polyacrylate was used as the water-retaining component 11 and L-proline was used as the moisture-absorbing component 12.

(Comparative Example 1)
The film 10 for application to biological tissue and the transfer sheet 20 of Comparative Example 1 were obtained in the same manner as in Example 1, except that the coating amount of the moisturizing layer 13 ^was approximately 6.0 g/ ^m2 and the coating amount of the protective layer was approximately 4.0 g/m2.
(Comparative Example 2)
The film 10 for application to biological tissue and the transfer sheet 20 of Comparative Example 2 were obtained in the same manner as in Example 1, except that the proportion of the moisture-retaining component 11 in the solid content of the moisturizing layer 13 was 40% by weight and the proportion of the organic polymer material was 60% by weight.
(Comparative Example 3)
The film 10 for application to biological tissue and the transfer sheet 20 of Comparative Example 3 were obtained in the same manner as in Example 1, except that the proportion of the hygroscopic component 12 in the solid content of the moisturizing layer 13 was 10% by weight and the proportion of the organic polymer material was 90% by weight.
(Comparative Example 4)
A coating film was formed by applying an aqueous solution in which polyvinyl alcohol (Gohsenol EG-40, manufactured by Mitsubishi Chemical) was dissolved at a concentration of 10% by mass to the film-forming substrate 30 using a bar coating method. The coating film was dried at 90°C for 2 minutes to form a coating film with a coating amount of about 0.8 g/m2.

Next, a solution in which cellulose acetate was dissolved in butyl acetate at a concentration of 10% by mass was applied onto the moisture-retaining layer 13 using a bar coating method to form a coating film. The coating film was dried at 90° C. for 2 minutes to form a protective layer 16 with a coating amount of about 0.2 g/m ^2. Thereafter, a transfer sheet 20 of Comparative Example 4 was obtained in the same manner as in Example 1.
The moisture retention rate and moisture absorption rate of the water-retaining component 11 and the moisture-absorbing component 12 are shown in Table 1.

The combinations and application amounts of moisture-retaining component 11 and moisture-absorbing component 12 for Examples 1 to 8 and Comparative Examples 1 to 4 are shown in Table 2.

(Evaluation Method)
[Skin transfer method]
500 μl of water was supplied as a supply liquid to the human skin, which was the transfer subject, and the supplied water was lightly spread with a finger. Then, the transfer sheet 20 was placed on the skin so that the surface of the biological tissue patch film 10 was in contact with the skin. Next, the test piece was pressed with a finger from above the support substrate 21 for 3 seconds. Then, the support substrate 21 was peeled off from the end of the test piece with a finger, and the biological tissue patch film 10 was transferred from the transfer sheet 20 to the skin.

[Evaluation of adhesion]
Five minutes after peeling off the support substrate 21, the functional layer on the skin was visually observed to check whether any deformation such as chipping, tearing, wrinkling, or twisting occurred, or whether any discomfort occurred due to application. If none of the above occurred, it was rated as "good," and if at least one of the above occurred, it was rated as "bad."

[Moisturizing sensation evaluation]
After the biological tissue patch film 10 was transferred, it was left in an environment of 23±1° C. and 50±5% humidity for one hour, and then the area where the biological tissue patch film 10 was attached was evaluated as to whether it was moist, unchanged, or dry compared to bare skin. If it was moist compared to bare skin, it was evaluated as "good," and if it was unchanged or drier than bare skin, it was evaluated as "bad."
The above-mentioned evaluation of the adhesion feeling and the moisturizing feeling were carried out by seven people, and when seven out of seven people gave a good evaluation, it was rated as ⊚, when six out of seven people gave a good evaluation, it was rated as ◯, and otherwise it was rated as ×. When both the adhesion feeling and the moisturizing feeling were rated as ◯ or ◎, it was judged that there was a moisturizing effect.

(Evaluation Results)
The results of the evaluation using the above evaluation method are shown in Table 3. All of Examples 1 to 8 were judged to have a moisturizing effect, whereas Comparative Example 1 had poor skin conformability due to the high application amount, resulting in a poor adhesion feeling, and also did not provide a moist feeling due to the poor conformability. Comparative Examples 2 to 4, which did not contain the water-retaining component 11 or the moisture-absorbing component 12, provided conformability but did not provide a sufficient moist feeling, and the moisturizing effect was inferior to that of the Examples. From the above results, it can be seen that the biological tissue patch film 10 of the present invention can achieve both improved conformability to the surface shape of the adherend and moisturizing function.

10, 10A, 10B, 10C, 10D... Biological tissue patch film 11... Moisture retention component 12... Moisture absorption component 13... Moisture retention layer 14... Moisture retention layer 15... Moisture absorption layer 16... Protective layer 20, 20A, 20B... Transfer sheet 21... Support substrate 30... Film-forming substrate Lq... Liquid material SK... Adherend

Claims (4)

A biological tissue patch film for application to skin, comprising a moisturizing layer,
the moisture retaining layer contains a moisture retaining component, a moisture absorbing component and a polymer material,
The mass per unit area is in the range of 0.1 g/m2 or ^more and 6.0 g/m2 ^or less ,
The moisture retaining layer includes a water retaining layer and a water absorbing layer in contact with the water retaining layer,
the water-retaining layer includes the water-retaining component and a first polymeric material;
the water-absorbing layer includes the moisture-absorbing component and a second polymeric material;
The moisture-retaining component contains sodium hyaluronate or sodium polyacrylate,
The moisture-absorbing component contains glycerin or L-proline;
The polymer material includes polyvinyl alcohol,
the content of each of the water-retaining component and the moisture-absorbing component in the moisturizing layer is in the range of 1% by mass or more and 80% by mass or less, and the total content of the water-retaining component and the moisture-absorbing component in the moisturizing layer is in the range of 1% by mass or more and 80% by mass or less,
A biological tissue patch film, comprising a protective layer made of cellulose acetate provided on the moisture retaining layer . 2. The biological tissue patch film according to claim 1 , wherein the moisture retention rate of said water-retaining component is 35% or more, and the moisture absorption rate of said moisture-absorbing component is 50% or more. 3. The biological tissue patch film according to claim 1, wherein the occlusion rate in the moisture retaining layer is 10% or less. A transfer sheet comprising a support substrate on one side of the outermost layer of the biological tissue patch film according to claim 1 .

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