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JP7569317B2 - Food saltiness enhancer, method for enhancing the saltiness of food, oil and fat composition, and method for producing food - Google Patents
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JP7569317B2 - Food saltiness enhancer, method for enhancing the saltiness of food, oil and fat composition, and method for producing food - Google Patents

Food saltiness enhancer, method for enhancing the saltiness of food, oil and fat composition, and method for producing food Download PDF

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JP7569317B2
JP7569317B2 JP2021528101A JP2021528101A JP7569317B2 JP 7569317 B2 JP7569317 B2 JP 7569317B2 JP 2021528101 A JP2021528101 A JP 2021528101A JP 2021528101 A JP2021528101 A JP 2021528101A JP 7569317 B2 JP7569317 B2 JP 7569317B2
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hydrolysate
fat
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JPWO2020255784A1 (en
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紗希 西村
俊 松澤
遼 岡部
茉莉 西村
貴士 佐野
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J Oil Mills Inc
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Seasonings (AREA)
  • Seeds, Soups, And Other Foods (AREA)
  • Noodles (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Cereal-Derived Products (AREA)
  • Edible Oils And Fats (AREA)

Description

本発明は、食品の塩味を増強することが可能な食品の塩味増強剤、食品の塩味増強方法、油脂組成物及び食品の製造方法に関する。The present invention relates to a food saltiness enhancer capable of enhancing the saltiness of food, a method for enhancing the saltiness of food, an oil and fat composition, and a method for producing food.

食塩は、食品の風味として非常に重要な塩味を付与し、食品の美味しさを増強してくれる調味料である。しかも、食塩は、身体を正常に機能させるための重要な役割を担っている。一方で、食塩の過剰摂取は、高血圧,心疾患,脳卒中,胃がん等の誘発の原因になると考えられている。 Salt is a seasoning that imparts a very important salty taste to food and enhances its deliciousness. Moreover, salt plays an important role in keeping the body functioning normally. However, excessive salt intake is thought to cause high blood pressure, heart disease, stroke, stomach cancer, and other diseases.

そこで、食塩の使用量を低減し、更に食品の塩味を損なわない減塩食品や減塩調味料の開発が進められている。As a result, efforts are underway to develop low-salt foods and seasonings that reduce the amount of salt used without impairing the saltiness of food.

例えば、特許文献1には、アルギニン及び/又はその塩、乳酸及び/又はその塩、並びにグルコン酸及び/又はその塩を原料に所定量添加する、塩味の増強された飲食品の製造方法が開示されている。For example, Patent Document 1 discloses a method for producing food and beverages with enhanced saltiness by adding predetermined amounts of arginine and/or its salt, lactic acid and/or its salt, and gluconic acid and/or its salt to raw materials.

特開2013-208057号公報JP 2013-208057 A

このような技術により飲食品に塩味を付与することは有効である。しかしながら、近年、減塩食品への関心はますます高くなっており、食塩の使用量を減らしても食品の塩味を損なわない技術の更なる開発が望まれている。 Using such technology is effective in imparting a salty taste to food and beverages. However, in recent years, interest in reduced-salt foods has been growing, and there is a need for further development of technology that does not impair the saltiness of foods even when the amount of salt used is reduced.

本発明の目的は、食品の塩味を増強させることができ、食塩の使用量の低減を可能にする新規の食品の塩味増強剤、食品の塩味増強方法、油脂組成物及び食品の製造方法を提供することにある。The object of the present invention is to provide a novel food saltiness enhancer that can enhance the saltiness of food and enable a reduction in the amount of salt used, a method for enhancing the saltiness of food, an oil and fat composition, and a method for producing food.

本願発明者等は、鋭意検討の結果、食品中に所定の食用油脂の加水分解物を含ませると塩味が増強することを見出した。 After extensive research, the inventors of the present application discovered that adding a hydrolysate of a specific edible oil or fat to a food product enhances its salty taste.

すなわち、本発明は、全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を有効成分とする食品の塩味増強剤を提供するものである。In other words, the present invention provides a salty taste enhancer for food, which contains as an active ingredient a hydrolysate of edible oil or fat in which the total fatty acids contain linoleic acid and oleic acid in an amount of 30% by mass or more.

また、本発明は、全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を食材に含ませることを特徴とする食品の塩味増強方法を提供するものである。The present invention also provides a method for enhancing the saltiness of food, which is characterized by including in a food ingredient a hydrolysate of an edible oil or fat, the total fatty acids of which contain linoleic acid and oleic acid in an amount of 30 mass% or more.

更に、本発明は、全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を0.01質量%以上20質量%以下含む油脂組成物を提供するものである。Furthermore, the present invention provides an oil and fat composition containing 0.01% by mass or more and 20% by mass or less of a hydrolysate of an edible oil and fat having a total fatty acid content of linoleic acid and oleic acid of 30% by mass or more.

更に、本発明は、全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を食材に含ませることを特徴とする食品の製造方法を提供するものである。 Furthermore, the present invention provides a method for producing a food, which is characterized by including in an ingredient a hydrolysate of an edible oil or fat, the total fatty acids of which contain linoleic acid and oleic acid in an amount of 30 mass% or more.

本発明の食品の塩味増強剤及び食品の塩味増強方法、油脂組成物並びに食品の製造方法では、上記合計が70質量%以上であることが好ましい。また、上記全脂肪酸中に含まれるオレイン酸が15質量%以上80質量%以下であることが好ましい。In the food saltiness enhancer, the method for enhancing the saltiness of food, the oil and fat composition, and the food manufacturing method of the present invention, it is preferable that the above total is 70% by mass or more. In addition, it is preferable that the oleic acid contained in the total fatty acids is 15% by mass or more and 80% by mass or less.

上記食用油脂は、大豆油、菜種油、コーン油及びオリーブ油から選ばれる一種又は二種以上であることが好ましい。The edible oil or fat is preferably one or more selected from soybean oil, rapeseed oil, corn oil and olive oil.

上記加水分解物は、リパーゼ処理物であることが好ましい。その場合、リパーゼは微生物由来であることが好ましい。The hydrolysate is preferably a lipase-treated product. In this case, the lipase is preferably derived from a microorganism.

また、上記加水分解物は加熱処理されたものであることが好ましい。 It is also preferable that the hydrolysate is heat-treated.

本発明の油脂組成物は、炒め用及び炊飯用から選ばれる一種又は二種であることが好ましい。The oil and fat composition of the present invention is preferably one or two types selected from those for frying and those for rice cooking.

本発明の食品の製造方法は、加水分解物を加熱する工程を含むことが好ましい。その場合、上記加水分解物を加熱する工程は、上記加水分解物を上記食材に含ませる前に行うことが好ましい。The method for producing a food product of the present invention preferably includes a step of heating the hydrolysate. In this case, the step of heating the hydrolysate is preferably carried out before the hydrolysate is incorporated into the food material.

ここで、本願の食品の塩味増強剤及び油脂組成物は、製造方法によって物の発明を特定しているが、下記の通り、当該塩味増強剤及び油脂組成物をその構造又は特性により直接特定することが不可能又はおよそ非実際的である事情が存在する。Here, the food saltiness enhancer and oil and fat composition of the present application are identified as product inventions by their manufacturing methods, but as described below, there are circumstances in which it is impossible or largely impractical to directly identify the saltiness enhancer and oil and fat composition by their structure or properties.

[不可能・非実際的事情の存在]
本願発明の食品の塩味増強剤及び油脂組成物は、全脂肪酸中に含まれるリノール酸及びオレイン酸の合計量が所定の値である食用油脂を加水分解して得られる加水分解物を有効成分とするものである。この加水分解物を構成する化学物質は、極めて多数であるとともに、加水分解の方法や分解条件(例えば、温度,時間,加水分解酵素の種類,加水分解後の処理)等に依存して異なるとも考えられる。従って、本願発明の食品の塩味増強剤又は油脂組成物を構成する極めて多数の化学物質のうち、どの化学物質が本発明の効果に寄与するのかを分析,特定することは、上述したような加水分解の方法及び条件の全ての組み合わせを試行して、含まれる化学物質を調べ、逐一塩味増強効果を確認することであり、現実的ではない回数の実験等を要するものである。すなわち、これは、不可能であるか、又は著しく過大な経済的支出や時間を要するためおよそ実際的ではない。
[Existence of impossible or impractical circumstances]
The food saltiness enhancer and oil and fat composition of the present invention contain as an active ingredient a hydrolysate obtained by hydrolyzing edible oils and fats in which the total amount of linoleic acid and oleic acid contained in the total fatty acids is a predetermined value. The chemical substances constituting this hydrolysate are extremely numerous, and are considered to differ depending on the hydrolysis method and decomposition conditions (e.g., temperature, time, type of hydrolytic enzyme, treatment after hydrolysis), etc. Therefore, to analyze and identify which chemical substances among the extremely numerous chemical substances constituting the food saltiness enhancer or oil and fat composition of the present invention contribute to the effects of the present invention, it is necessary to try all combinations of the hydrolysis methods and conditions as described above, examine the chemical substances contained, and confirm the saltiness enhancement effect one by one, which requires an unrealistic number of experiments, etc. In other words, this is impossible or is not practical because it requires significantly excessive economic expenditure and time.

本発明の食品の塩味増強剤、食品の塩味増強方法及び油脂組成物によれば、適用する食品の塩味を増強させることができる。また、本発明の食品の製造方法によれば、本発明の塩味増強剤又は油脂組成物を食品に含ませて、食品の塩味を増強させることができる。よって、従来よりも食塩の使用量が低減され、さらに塩味も感じられる食品を実現することができる。 The food saltiness enhancer, method for enhancing the saltiness of food, and oil and fat composition of the present invention can enhance the saltiness of the food to which they are applied. Furthermore, according to the food production method of the present invention, the saltiness of food can be enhanced by including the saltiness enhancer or oil and fat composition of the present invention in food. This allows for a reduction in the amount of salt used compared to conventional methods, and makes it possible to produce food that still tastes salty.

以下に、本発明の一実施の形態に係る食品の塩味増強剤及び食品の塩味増強方法、油脂組成物並びに食品の製造方法について説明する。 Below, we will explain a food saltiness enhancer and a method for enhancing the saltiness of food, an oil and fat composition, and a method for producing food according to one embodiment of the present invention.

本実施の形態の食品の塩味増強剤は、食用油脂の全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上、好ましくは60質量%以上、更に好ましくは70質量%以上、更により好ましくは73質量%以上含まれる、上記食用油脂の加水分解物を有効成分とする食品の塩味増強剤である。リノール酸及びオレイン酸の合計の上限は特に限定されないが、例えば、95質量%以下、好ましくは90質量%以下、より好ましくは88質量%以下である。The food saltiness enhancer of this embodiment is a food saltiness enhancer containing, as an active ingredient, a hydrolysate of edible fats and oils, in which the total amount of linoleic acid and oleic acid in the total fatty acids of the edible fats and oils is 30% by mass or more, preferably 60% by mass or more, more preferably 70% by mass or more, and even more preferably 73% by mass or more. The upper limit of the total amount of linoleic acid and oleic acid is not particularly limited, but is, for example, 95% by mass or less, preferably 90% by mass or less, and more preferably 88% by mass or less.

上記食用油脂の全脂肪酸中に含まれるオレイン酸は、15質量%以上80質量%以下であることが好ましく、20質量%以上80質量%以下であることがより好ましく、20質量%以上70質量%以下であることが更に好ましく、20質量%以上67質量%以下であることが更により好ましく、20質量%以上25質量%以下であることが特に好ましい。The amount of oleic acid contained in the total fatty acids of the edible oil or fat is preferably 15% by mass or more and 80% by mass or less, more preferably 20% by mass or more and 80% by mass or less, even more preferably 20% by mass or more and 70% by mass or less, even more preferably 20% by mass or more and 67% by mass or less, and particularly preferably 20% by mass or more and 25% by mass or less.

また、上記食用油脂の全脂肪酸中に含まれるリノール酸は、4質量%以上60質量%以下であることが好ましく、16質量%以上60質量%以下であることがより好ましく、16質量%以上58質量%以下であることが更に好ましく、45質量%以上58質量%以下であることが更により好ましい。In addition, the amount of linoleic acid contained in the total fatty acids of the edible oil or fat is preferably 4% by mass or more and 60% by mass or less, more preferably 16% by mass or more and 60% by mass or less, even more preferably 16% by mass or more and 58% by mass or less, and even more preferably 45% by mass or more and 58% by mass or less.

本実施の形態の食品の塩味増強剤は、食品に添加して使用するのに適した形態であればよく、具体的には、食用油脂で希釈した液状形態、粉末状形態、液状形態よりも油脂成分の配合量が少ない溶液状、ゲル状、顆粒状、固形状等の種々の形態とすることができる。The food saltiness enhancer of this embodiment may be in any form suitable for use by adding it to food, and specifically may be in various forms such as a liquid form diluted with edible fats and oils, a powder form, a solution form containing a smaller amount of fat and oil components than the liquid form, a gel form, a granular form, a solid form, etc.

上記食用油脂としては、例えば、大豆油,菜種油(高オレイン酸タイプを含む),コーン油,オリーブ油,パームオレイン,グレープシード油,ゴマ油,米油,えごま油及び亜麻仁油が挙げられる。これらのなかでも、食用油脂は、大豆油,菜種油,コーン油及びオリーブ油から選ばれる一種又は二種以上であることが好ましく、大豆油,菜種油(高オレイン酸タイプを含まない)及びコーン油から選ばれる一種又は二種以上であることがより好ましく、大豆油及び菜種油(高オレイン酸タイプを含まない)から選ばれる一種又は二種であることが更に好ましく、大豆油であることが最も好ましい。食用油脂は、一種単独でも二種以上を併用した混合油であってもよい。混合油の場合、混合油全体におけるリノール酸及び/又はオレイン酸含有量が上述した範囲内であればよい。Examples of the edible oils and fats include soybean oil, rapeseed oil (including high oleic acid type), corn oil, olive oil, palm olein, grapeseed oil, sesame oil, rice oil, perilla oil and linseed oil. Among these, the edible oils and fats are preferably one or more selected from soybean oil, rapeseed oil, corn oil and olive oil, more preferably one or more selected from soybean oil, rapeseed oil (not including high oleic acid type) and corn oil, even more preferably one or two selected from soybean oil and rapeseed oil (not including high oleic acid type), and most preferably soybean oil. The edible oils and fats may be one type alone or a mixed oil containing two or more types in combination. In the case of a mixed oil, it is sufficient that the linoleic acid and/or oleic acid content in the entire mixed oil is within the above-mentioned range.

食用油脂の上昇融点は、10℃以下であることが好ましく、0℃以下であることがより好ましい。このような上昇融点とすることで、後述する加水分解を効率よく行うことができる。なお、上昇融点は、基準油脂分析試験法の3.2.2.2-2013に準拠して測定することができる。The slip melting point of edible fats and oils is preferably 10°C or lower, and more preferably 0°C or lower. By setting the slip melting point at such a level, the hydrolysis described below can be carried out efficiently. The slip melting point can be measured in accordance with 3.2.2.2-2013 of the Standard Methods for the Analysis of Fats, Oils and Oils.

上記「加水分解物」とは、食用油脂をグリセリンと脂肪酸とに加水分解して得られるもののことを指す。加水分解の方法や条件は特に限定されないが、加水分解の方法としては、例えば、Twitchell法,中圧(1~4MPa)触媒分解,連続高圧(5~6MPa)分解及び加水分解酵素を用いた方法が知られている。The above-mentioned "hydrolysate" refers to a product obtained by hydrolyzing edible oils and fats into glycerin and fatty acids. The hydrolysis method and conditions are not particularly limited, but known hydrolysis methods include, for example, the Twitchell method, medium pressure (1-4 MPa) catalytic hydrolysis, continuous high pressure (5-6 MPa) hydrolysis, and a method using a hydrolase enzyme.

上記脂肪酸の加水分解による分離率は100%に限定されない。分離率は、好ましくは60%以上100%以下であり、より好ましくは85%以上100%以下であり、更に好ましくは88%以上100%以下であり、更により好ましくは90%以上100%以下である。The separation rate by hydrolysis of the above fatty acids is not limited to 100%. The separation rate is preferably 60% or more and 100% or less, more preferably 85% or more and 100% or less, even more preferably 88% or more and 100% or less, and even more preferably 90% or more and 100% or less.

上記加水分解物の酸価は、好ましくは150以上220以下であり、より好ましくは170以上210以下である。酸価は、基準油脂分析試験法の2.3.1酸価(日本油化学会)に準拠して測定することができる。The acid value of the hydrolyzate is preferably 150 or more and 220 or less, more preferably 170 or more and 210 or less. The acid value can be measured in accordance with 2.3.1 Acid Value of the Standard Method for Analysis of Fats, Oils and Related Materials (Japan Oil Chemists' Society).

本実施の形態の塩味増強剤は、加水分解酵素としてリパーゼを用いて得られたリパーゼ処理物を含んでいることが好ましい。加水分解酵素は、一種単独でも二種以上を併用してもよい。上記リパーゼは、特に限定されるものではない。使用されるリパーゼは、例えば、微生物由来,動物由来又は植物由来のものであり、微生物由来であることが好ましい。微生物としては、例えば、糸状菌(Aspergillus awamori, Aspergillus niger, Aspergillus oryzae, Aspergillus phoenicis, Aspergillus usamii, Geotrichum candidum, Humicola, Mucor javanicus, Mucor miehei, Penicillium camembertii, Penicillium chrysogenum, Penicillum roquefortii, Rhizomucor miehei, Rhizopus delemar, Rhizopus japonicus, Rhizopus miehei, Rhizopus niveus, Rhizopus oryzae)、放線菌(Streptomyces)、細菌(Alcaligenes, Arthrobactor, Chromobacterium viscosum, Pseudomonas, Serratia marcescens)及び酵母(Candida)が挙げられ、Candida属由来のリパーゼが特に好ましい。The salty taste enhancer of the present embodiment preferably contains a lipase-treated product obtained by using lipase as a hydrolase. The hydrolase may be used alone or in combination of two or more kinds. The lipase is not particularly limited. The lipase used is, for example, derived from a microorganism, an animal, or a plant, and is preferably derived from a microorganism. Examples of microorganisms include filamentous fungi (Aspergillus awamori, Aspergillus niger, Aspergillus oryzae, Aspergillus phoenicis, Aspergillus usamii, Geotrichum candidum, Humicola, Mucor javanicus, Mucor miehei, Penicillium camembertii, Penicillium chrysogenum, Penicillum roquefortii, Rhizomucor miehei, Rhizopus delemar, Rhizopus japonicus, Rhizopus miehei, Rhizopus niveus, Rhizopus oryzae), actinomycetes (Streptomyces), bacteria (Alcaligenes, Arthrobactor, Chromobacterium viscosum, Pseudomonas, Serratia marcescens) and yeasts (Candida), and lipases derived from the genus Candida are particularly preferred.

加水分解酵素としてリパーゼを用いる場合、加水分解の温度は、リパーゼが失活しない温度であればよく、好ましくは20℃以上60℃以下、より好ましくは25℃以上55℃以下、更に好ましくは30℃以上50℃以下である。また、時間は、好ましくは0.1時間以上84時間以下、より好ましくは0.1時間以上48時間以下、更に好ましくは0.2時間以上48時間以下、更により好ましくは0.3時間以上30時間以下である。食用油脂に対する加水分解酵素の添加量は0.1~40質量%程度が好ましい。加水分解後は、遠心分離を行って加水分解物を回収することが好ましく、回収した加水分解物を水洗し、再度遠心分離することも好ましい。When lipase is used as the hydrolase, the hydrolysis temperature may be any temperature at which lipase is not inactivated, and is preferably 20°C to 60°C, more preferably 25°C to 55°C, and even more preferably 30°C to 50°C. The time is preferably 0.1 hours to 84 hours, more preferably 0.1 hours to 48 hours, even more preferably 0.2 hours to 48 hours, and even more preferably 0.3 hours to 30 hours. The amount of hydrolase added to the edible oil or fat is preferably about 0.1 to 40% by mass. After hydrolysis, it is preferable to collect the hydrolyzate by centrifugation, and it is also preferable to wash the collected hydrolyzate with water and centrifuge it again.

本実施の形態の塩味増強剤は、上記食用油脂の加水分解物を0.001質量%以上100質量%以下含有していることが好ましく、0.005質量%以上100質量%以下がより好ましく、0.01質量%以上100質量%以下が更に好ましく、0.1質量%以上100質量%以下が更により好ましい。The salty taste enhancer of this embodiment preferably contains 0.001% by mass or more and 100% by mass or less of the hydrolysate of the above-mentioned edible oil or fat, more preferably 0.005% by mass or more and 100% by mass or less, even more preferably 0.01% by mass or more and 100% by mass or less, and even more preferably 0.1% by mass or more and 100% by mass or less.

食品の塩味増強剤には、所望する塩味増強の機能性を損なわない範囲で、食用油脂に通常添加される助剤が適宜配合されていてもよい。助剤としては、酸化防止剤,消泡剤,乳化剤,香料,風味付与剤,色素,生理活性物質等が挙げられる。具体的には、例えば、アスコルビン酸脂肪酸エステル,リグナン,コエンザイムQ,γ-オリザノール,トコフェロール,シリコーンである。 The salty taste enhancer for food may contain auxiliary agents that are usually added to edible oils and fats, as long as the functionality of the desired salty taste enhancement is not impaired. Examples of auxiliary agents include antioxidants, antifoaming agents, emulsifiers, fragrances, flavoring agents, colorants, and biologically active substances. Specific examples include ascorbic acid fatty acid esters, lignans, coenzyme Q, γ-oryzanol, tocopherol, and silicones.

上述した加水分解物は、例えば食用油脂に添加して油脂組成物とすることができる。油脂組成物中の加水分解物の含有量は、好ましくは0.01質量%以上20質量%以下、より好ましくは0.05質量%以上15質量%以下、更に好ましくは0.1質量%以上10質量%以下、更により好ましくは0.2質量%以上10質量%以下である。The above-mentioned hydrolysate can be added to, for example, edible fats and oils to form an oil and fat composition. The content of the hydrolysate in the oil and fat composition is preferably 0.01% by mass or more and 20% by mass or less, more preferably 0.05% by mass or more and 15% by mass or less, even more preferably 0.1% by mass or more and 10% by mass or less, and even more preferably 0.2% by mass or more and 10% by mass or less.

上記油脂組成物の食用油脂には、例えば、菜種油(高オレイン酸タイプを含む),大豆油,パーム油,パーム核油,コーン油,オリーブ油,ゴマ油,紅花油,ヒマワリ油,綿実油,米油,落花生油,ヤシ油,カカオ脂等の植物油脂、牛脂,豚脂,鶏脂,乳脂等の動物油脂及び中鎖脂肪酸トリグリセリドが用いられる。加えて、これらの分別油(パーム油の中融点部、パーム油の軟質分別油、パーム油の硬質分別油等),エステル交換油、水素添加油等の加工油脂も使用することができる。食用油脂は、一種単独でも二種以上が混合されていてもよい。The edible fats and oils in the oil and fat composition include, for example, vegetable fats and oils such as rapeseed oil (including high oleic acid type), soybean oil, palm oil, palm kernel oil, corn oil, olive oil, sesame oil, safflower oil, sunflower oil, cottonseed oil, rice oil, peanut oil, coconut oil, and cocoa butter, animal fats and oils such as beef tallow, lard, chicken fat, and milk fat, and medium-chain fatty acid triglycerides. In addition, processed fats and oils such as fractionated oils (mid-melting point part of palm oil, soft fractionated oil of palm oil, hard fractionated oil of palm oil, etc.), transesterified oils, and hydrogenated oils can also be used. The edible fats and oils may be used alone or in a mixture of two or more types.

また、油脂組成物には、上記塩味増強剤と同様に、所望する塩味増強の機能性を損なわない範囲で、食用油脂に通常添加される助剤が適宜配合されていてもよい。In addition, the oil and fat composition may contain, as appropriate, auxiliary agents that are typically added to edible oils and fats, as long as the desired functionality of enhancing saltiness is not impaired, similar to the saltiness enhancer described above.

本実施の形態の油脂組成物は、その使い方は特に限定されないが、好ましくは炒め用及び炊飯用から選ばれる一種又は二種であり、より好ましくは炒め用である。The oil and fat composition of this embodiment is not particularly limited in its usage, but is preferably used for one or two purposes selected from stir-frying and rice cooking, and more preferably for stir-frying.

上記加水分解物は、また、食品に含ませることにより当該食品の塩味を増強することができる。すなわち、少ない食塩の使用量でも高い塩分を有しているかのように塩味を感じることができる。食品中に含まれる加水分解物の割合は、0.0001質量%以上1質量%以下であることが好ましく、0.001質量%以上1質量%以下であることがより好ましい。上記食品は、食塩又は塩味を感じる成分を含むものであれば特に限定されない。例えば、炒め調理品,焼き調理品,蒸し調理品,茹で調理品,炊き調理品,煮込み調理品又は調味料等であり、炒め調理品であることが好ましい。具体例としては、例えば、パスタ料理,炒飯,野菜炒め,焼き肉,焼き魚,温野菜,中華まん,焼売,肉団子,ハム,ソーセージ,おにぎり,炊き込みご飯,スープ,ルウ,チーズ,スナック菓子,和菓子,パンが挙げられる。The above hydrolysate can also be added to food to enhance the saltiness of the food. In other words, even if a small amount of salt is used, the saltiness of the food can be felt as if it had a high salt content. The ratio of the hydrolysate contained in the food is preferably 0.0001% by mass to 1% by mass, and more preferably 0.001% by mass to 1% by mass. The above food is not particularly limited as long as it contains salt or a component that gives a salty taste. For example, it is a stir-fried dish, a baked dish, a steamed dish, a boiled dish, a cooked dish, a stewed dish, or a seasoning, and preferably a stir-fried dish. Specific examples include pasta dishes, fried rice, stir-fried vegetables, grilled meat, grilled fish, warm vegetables, Chinese buns, shumai, meatballs, ham, sausages, rice balls, cooked rice, soup, roux, cheese, snacks, Japanese sweets, and bread.

食品に含まれる加水分解物は、加熱処理されてから喫食されることが好ましい。加熱処理については、加水分解物をそのまま加熱してもよいし、食用油脂で希釈した液状形態としてから加熱してもよいし、粉末状形態,溶液状,ゲル状,顆粒状,固形状等としてから加熱してもよい。加熱条件等は特に限定されるものではない。加熱に関しては、予め加水分解物を加熱処理し、加熱処理された加水分解物を食品又は調理する食材に加えてもよい。また、食材を加熱調理する際に食材とともに加水分解物を加熱処理し、食品に含ませるようにしてもよい。It is preferable that the hydrolysate contained in the food is heat-treated before being consumed. Regarding the heat treatment, the hydrolysate may be heated as is, or may be diluted with edible oils and fats to form a liquid form and then heated, or may be heated in a powder form, solution form, gel form, granule form, solid form, etc. The heating conditions, etc. are not particularly limited. Regarding heating, the hydrolysate may be heat-treated in advance, and the heat-treated hydrolysate may be added to the food or ingredients to be cooked. Also, when cooking ingredients, the hydrolysate may be heat-treated together with the ingredients and then included in the food.

以下に、本発明の実施例を示すことにより、本発明をより詳細に説明する。しかしながら、本発明は、以下の実施例に限定されるものではない。The present invention will be described in more detail below by showing examples of the present invention. However, the present invention is not limited to the following examples.

本実施例において使用した材料及び調理器具は、以下の通りである。
(食用油脂;記載のない食用油脂の上昇融点は0℃以下)
・大豆油:「AJINOMOTOコクとうまみの大豆の油」、株式会社J-オイルミルズ製
・菜種油:「AJINOMOTOさらさらキャノーラ油」、株式会社J-オイルミルズ製
・高オレイン酸菜種油:「社内調製品」、株式会社J-オイルミルズ製
・コーン油:「AJINOMOTO胚芽の恵みコーン油」、株式会社J-オイルミルズ製
・精製オリーブ油:「社内調製品」、株式会社J-オイルミルズ製
・乳脂:「バターオイル」、FrieslandCampina Butter社製(上昇融点15℃以上)
・中鎖脂肪酸トリグリセリド(MCT):「MT-60」、花王株式会社製
(加水分解酵素)
・リパーゼ:Lipase AY 「アマノ」30SD(Candida属微生物由来)、天野エンザイム株式会社製
(食材)
・パスタ乾麺:「マ・マーチャック付結束スパゲティ1.6mm」、日清フーズ株式会社製
(加熱調理器)
・IHヒーター:「KZ-PH33」、パナソニック株式会社製
The materials and cooking utensils used in this example are as follows:
(Edible fats and oils: The melting point of edible fats and oils not specified is below 0°C)
・Soybean oil: "AJINOMOTO Rich and Flavorful Soybean Oil", manufactured by J-Oil Mills Co., Ltd. ・Rapeseed oil: "AJINOMOTO Smooth Canola Oil", manufactured by J-Oil Mills Co., Ltd. ・High oleic acid rapeseed oil: "In-house preparation", manufactured by J-Oil Mills Co., Ltd. ・Corn oil: "AJINOMOTO Germ Blessing Corn Oil", manufactured by J-Oil Mills Co., Ltd. ・Refined olive oil: "In-house preparation", manufactured by J-Oil Mills Co., Ltd. ・Milk fat: "Butter oil", manufactured by FrieslandCampina Butter (slip melting point 15°C or higher)
・Medium-chain triglyceride (MCT): "MT-60", manufactured by Kao Corporation (hydrolase enzyme)
Lipase: Lipase AY "Amano" 30SD (derived from Candida genus microorganisms), manufactured by Amano Enzyme Co., Ltd. (food ingredient)
・Dried pasta: "Ma-Ma chuck-attached spaghetti 1.6 mm", manufactured by Nissin Foods Inc. (heat cooker)
・IH heater: "KZ-PH33", manufactured by Panasonic Corporation

<実施例1>
本実施例においては、食用油脂として大豆油(実施例1-1),菜種油(実施例1-2),高オレイン酸菜種油(実施例1-3),コーン油(実施例1-4),精製オリーブ油(実施例1-5),乳脂(比較例1-1)又は中鎖脂肪酸トリグリセリド(比較例1-2)を用い、以下の(a)~(f)の手順に従って食用油脂を加水分解し、加水分解物を作製した。
Example 1
In the present examples, soybean oil (Example 1-1), rapeseed oil (Example 1-2), high oleic acid rapeseed oil (Example 1-3), corn oil (Example 1-4), refined olive oil (Example 1-5), milk fat (Comparative Example 1-1) or medium-chain fatty acid triglyceride (Comparative Example 1-2) was used as the edible fat or oil, and the edible fat or oil was hydrolyzed to produce a hydrolysate according to the following procedures (a) to (f).

(a)食用油脂20gと、水12gと、リパーゼ0.2gとを50mLのチューブに添加して蓋を閉めた。
(b)ウォーターバス(UNI ACE SHAKER NCS-1300、東京理化器械株式会社製)を用いて、(a)のチューブを40℃で24時間振盪させた。
(c)遠心分離機(ユニバーサル遠心機5911、株式会社クボタ製)を用いて、24℃で(b)の内容物を遠心分離(3000rpm、5min)した。
(d)(c)の上清15gを採取した。
(e)(d)の上清に水6gを添加したものを、再び24℃で遠心分離(3000rpm、5min)した。
(f)(e)の上清10gを回収して加水分解物を得た。
(a) 20 g of edible oil, 12 g of water, and 0.2 g of lipase were added to a 50 mL tube and the lid was closed.
(b) The tube of (a) was shaken at 40° C. for 24 hours using a water bath (UNI ACE SHAKER NCS-1300, manufactured by Tokyo Rikakikai Co., Ltd.).
(c) Using a centrifuge (Universal Centrifuge 5911, manufactured by Kubota Corporation), the contents of (b) were centrifuged (3000 rpm, 5 min) at 24°C.
(d) 15 g of the supernatant of (c) was collected.
(e) 6 g of water was added to the supernatant of (d), and the mixture was centrifuged again at 24° C. (3,000 rpm, 5 min).
(f) 10 g of the supernatant of (e) was collected to obtain a hydrolysate.

得られた加水分解物の酸価を「基準油脂分析試験法2.3.1酸価」(日本油化学会)に準拠して測定した。また、この酸価の値を用いて脂肪酸純度(すなわち、上述した分離率)を求めた。具体的には、酸価の値より1g中の遊離脂肪酸の物質量を算出し、各食用油脂の脂肪酸組成より求めた平均分子量を用いて脂肪酸純度(w/w)を算出した。The acid value of the obtained hydrolysate was measured in accordance with "Standard Methods for the Analysis of Fats, Oils and Fat, 2.3.1 Acid Value" (Japan Oil Chemists' Society). This acid value was used to determine the fatty acid purity (i.e., the separation rate described above). Specifically, the amount of free fatty acid per gram was calculated from the acid value, and the fatty acid purity (w/w) was calculated using the average molecular weight determined from the fatty acid composition of each edible oil.

各食用油脂の脂肪酸組成、平均分子量及び得られた結果について表1に示す。The fatty acid composition, average molecular weight and results obtained for each edible oil are shown in Table 1.

Figure 0007569317000001
Figure 0007569317000001

表1から分かるように、大豆油,菜種油,高オレイン酸菜種油,コーン油及び精製オリーブ油では、リパーゼを用いて90%以上の脂肪酸純度で食用油脂から脂肪酸が遊離されることが確認された。また、比較例の乳脂及び中鎖脂肪酸トリグリセリドについても、80%以上の脂肪酸純度で食用油脂から脂肪酸が遊離されていた。As can be seen from Table 1, it was confirmed that in soybean oil, rapeseed oil, high oleic rapeseed oil, corn oil and refined olive oil, fatty acids were released from edible oils and fats using lipase with a fatty acid purity of 90% or more. In addition, in the comparative milk fat and medium-chain triglyceride, fatty acids were released from edible oils and fats with a fatty acid purity of 80% or more.

<実施例2>
本実施例では、実施例1-1~1-5及び比較例1-1,1-2の加水分解物を含ませたパスタ料理(実施例2-1~2-5及び比較例2-1,2-2)の塩味について評価した。
Example 2
In this example, the saltiness of pasta dishes containing the hydrolysates of Examples 1-1 to 1-5 and Comparative Examples 1-1 and 1-2 (Examples 2-1 to 2-5 and Comparative Examples 2-1 and 2-2) was evaluated.

パスタ料理は、以下の(a)~(d)の手順に従って調理した。
(a)菜種油99質量部に対して実施例1-1~1-5及び比較例1-1,1-2のいずれかの加水分解物1質量部を添加した7種類の油脂組成物を7.5gずつ用意した。対照として、菜種油7.5gを用意した。
(b)パスタ乾麺を1.5質量%の食塩水を用いて7分間茹で、湯切りした。
(c)(a)で用意した油脂組成物又は菜種油をフライパンに引き、みじん切りしたニンニク2.5gを加え、IHヒーターを用いて温度強度3(100~120℃)で10分間炒め、自然冷却した。
(d)(c)のフライパンに(b)で茹でたパスタ100gとエクストラバージンオリーブ油(「AJINOMOTOオリーブオイルエクストラバージン」、株式会社J-オイルミルズ製)7.5gとを加え、混ぜ合わせて(油脂組成物を用いた)実施例2-1~2-5及び比較例2-1,2-2並びに(菜種油のみを用いた)対照のパスタ料理を得た。
The pasta dishes were cooked according to the following steps (a) to (d).
(a) Seven types of oil and fat compositions were prepared by adding 1 part by mass of any of the hydrolysates of Examples 1-1 to 1-5 and Comparative Examples 1-1 and 1-2 to 99 parts by mass of rapeseed oil in an amount of 7.5 g each. As a control, 7.5 g of rapeseed oil was prepared.
(b) Dried pasta noodles were boiled in 1.5% by mass salt water for 7 minutes and then drained.
(c) The oil composition prepared in (a) or rapeseed oil was placed in a frying pan, 2.5 g of minced garlic was added, and the mixture was fried for 10 minutes at temperature intensity 3 (100-120°C) using an induction heater, and then naturally cooled.
(d) 100 g of the pasta boiled in (b) and 7.5 g of extra virgin olive oil ("AJINOMOTO Olive Oil Extra Virgin", manufactured by J-Oil Mills, Inc.) were added to the frying pan in (c) and mixed to obtain the pasta dishes of Examples 2-1 to 2-5 (which used an oil and fat composition) and Comparative Examples 2-1 and 2-2, as well as the control (which used only rapeseed oil).

得られた実施例2-1~2-5及び比較例2-1,2-2のパスタ料理について、対照のパスタ料理との比較により塩味を官能評価した。評価は、5名の専門パネルにより以下の採点基準で行い、全員の官能評価値の平均値を求めた。結果を表2に示す。The obtained pasta dishes of Examples 2-1 to 2-5 and Comparative Examples 2-1 and 2-2 were subjected to a sensory evaluation of saltiness in comparison with the control pasta dishes. The evaluation was performed by a panel of five experts using the following scoring criteria, and the average of the sensory evaluation scores of all the panelists was calculated. The results are shown in Table 2.

+6:対照よりもかなり強い
+5:対照よりも相当強い
+4:対照よりも強い
+3:対照よりも比較的強い
+2:対照よりもやや強い
+1:対照よりもわずかに強い
0:対照と同等
-1:対照よりもやや弱い
-2:対照よりも弱い
-3:対照よりもかなり弱い
+6: Much stronger than the control +5: Much stronger than the control +4: Stronger than the control +3: Relatively stronger than the control +2: Slightly stronger than the control +1: Slightly stronger than the control 0: Equivalent to the control -1: Slightly weaker than the control -2: Weaker than the control -3: Much weaker than the control

Figure 0007569317000002
Figure 0007569317000002

表2から分かるように、本発明に従うリノール酸及びオレイン酸の合計量を有する実施例1-1~1-5の加水分解物を含むパスタ料理は、加水分解物を含んでいない場合と比較して塩味が増強された。最も塩味が増強されたのは食用油脂として大豆油を用いた実施例2-1のパスタ料理であり、2番目に塩味増強効果が高いのは、食用油脂として菜種油を用いた実施例2-2のパスタ料理であり、3番目に塩味増強効果が高いのは、食用油脂としてコーン油を用いた実施例2-4のパスタ料理であった。これに対し、本発明に従うリノール酸及びオレイン酸の合計量を有していない比較例1-1,1-2の加水分解物を含むパスタ料理は、加水分解物を含んでいない場合と同等の塩味しか得られなかった。As can be seen from Table 2, the pasta dishes containing the hydrolysates of Examples 1-1 to 1-5, which have the total amount of linoleic acid and oleic acid according to the present invention, had an enhanced saltiness compared to those not containing the hydrolysate. The pasta dish with the most enhanced saltiness was the pasta dish of Example 2-1, which used soybean oil as the edible oil, the pasta dish with the second highest saltiness enhancement effect was the pasta dish of Example 2-2, which used rapeseed oil as the edible oil, and the pasta dish with the third highest saltiness enhancement effect was the pasta dish of Example 2-4, which used corn oil as the edible oil. In contrast, the pasta dishes containing the hydrolysates of Comparative Examples 1-1 and 1-2, which do not have the total amount of linoleic acid and oleic acid according to the present invention, only had the same saltiness as those not containing the hydrolysate.

<実施例3>
本実施例では、油脂組成物中における加水分解物の含有量を検討した。より詳細には、油脂組成物中における加水分解物の含有量を変化させて、加水分解物を含ませたパスタ料理(実施例3-1~3-4)の塩味について評価した。
Example 3
In this example, the content of hydrolysate in the oil and fat composition was examined. More specifically, the content of hydrolysate in the oil and fat composition was changed, and the saltiness of pasta dishes containing the hydrolysate (Examples 3-1 to 3-4) was evaluated.

パスタ料理は、以下の(a)~(e)の手順に従って調理した。各材料の使用量は表3に記載の通りである。なお、食品の総質量は、後述する茹でたパスタの質量を220gとして計算した。The pasta dishes were cooked according to the following steps (a) to (e). The amounts of each ingredient used are as shown in Table 3. The total mass of the food was calculated based on the mass of boiled pasta (described below) being 220 g.

Figure 0007569317000003
Figure 0007569317000003

(a)表3に記載の油脂組成物、油脂又は混合油脂をフライパンに添加した。
(b)(a)のフライパンにニンニクを加え、IHヒーターを用いて温度強度3(100~120℃)で10分間炒め、自然冷却した。
(c)(b)のフライパンに、エクストラバージンオリーブ油と食塩とを加えて混ぜ合わせた。
(d)パスタ乾麺を水で7分間茹で、湯切りした。
(e)(c)のフライパンに(d)で茹でたパスタを加え、混ぜ合わせて実施例3-1~3-4及び比較例3-1,3-2のパスタ料理を得た。
(a) The oil or fat composition, oil or fat mixture shown in Table 3 was added to a frying pan.
(b) Garlic was added to the frying pan (a) and fried for 10 minutes at temperature intensity 3 (100-120°C) using an induction heater, and then allowed to cool naturally.
(c) Extra virgin olive oil and salt were added to the frying pan (b) and mixed.
(d) Dried pasta was boiled in water for 7 minutes and then drained.
(e) The pasta boiled in (d) was added to the frying pan in (c) and mixed to obtain the pasta dishes of Examples 3-1 to 3-4 and Comparative Examples 3-1 and 3-2.

得られた実施例3-1~3-4のパスタ料理について、比較例3-1のパスタ料理(対照)との比較により塩味を官能評価した。評価は、2名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、2名の官能評価値の平均値を求めた。結果を表4に示す。The obtained pasta dishes of Examples 3-1 to 3-4 were subjected to a sensory evaluation of saltiness in comparison with the pasta dish (control) of Comparative Example 3-1. The evaluation was performed by two expert panelists according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the two panelists was calculated. The results are shown in Table 4.

Figure 0007569317000004
Figure 0007569317000004

表4に示したように、油脂組成物中における加水分解物の割合が0.1質量%以上10質量%以下において、加水分解物を含ませていない比較例3-1のパスタ料理よりも塩味が増強していることが確認された。また、この結果から、油脂組成物中における好ましい加水分解物の割合は0.2質量%以上10質量%以下であり、1質量%以上10質量%以下であればより好ましく、1質量%が特に好ましいことが分かる。As shown in Table 4, it was confirmed that when the proportion of hydrolysate in the oil and fat composition was 0.1% by mass or more and 10% by mass or less, the saltiness was enhanced compared to the pasta dish of Comparative Example 3-1, which did not contain any hydrolysate. Furthermore, from these results, it can be seen that the preferred proportion of hydrolysate in the oil and fat composition is 0.2% by mass or more and 10% by mass or less, more preferably 1% by mass or more and 10% by mass or less, with 1% by mass being particularly preferred.

更に、実施例3-3及び比較例3-2のパスタ料理の塩味の強さをビジュアルアナログスケール(VAS)で評価した。0を「全く塩味なし」、30を「この上なく塩味が強い」とする0~30のスケールとし、10名の専門パネルが塩味の程度を表すところに印を付け、その平均値を算出し、標準偏差を求めた。結果を表5に示す。 Furthermore, the saltiness of the pasta dishes of Example 3-3 and Comparative Example 3-2 was evaluated using a visual analog scale (VAS). The scale was 0 to 30, with 0 being "not salty at all" and 30 being "extremely salty." A panel of 10 experts marked the area that represented the degree of saltiness, calculated the average, and determined the standard deviation. The results are shown in Table 5.

Figure 0007569317000005
Figure 0007569317000005

表5から分かるように、本発明の加水分解物を含ませた実施例3-3のパスタ料理は、加水分解物を含ませていない比較例3-2のパスタ料理に比べて塩味が増強されたことを示す結果であった。なお、このデータについて有意水準10%でt検定を行った結果、有意差が認められた(有意確率p=0.082)。As can be seen from Table 5, the results show that the pasta dish of Example 3-3, which contained the hydrolysate of the present invention, had an enhanced salty taste compared to the pasta dish of Comparative Example 3-2, which did not contain the hydrolysate. Furthermore, a t-test was performed on this data at a significance level of 10%, and a significant difference was found (significance probability p=0.082).

<実施例4>
本実施例では、加水分解物を加熱した場合の効果について検討した。より詳細には、加水分解物を加熱してから含ませたパスタ料理(実施例4-1)と、加水分解物を加熱することなく含ませたパスタ料理(実施例4-2)との塩味について評価した。
Example 4
In this example, the effect of heating the hydrolysate was examined. More specifically, the saltiness of a pasta dish containing a heated hydrolysate (Example 4-1) and a pasta dish containing a hydrolysate without heating (Example 4-2) was evaluated.

パスタ料理は、以下の(a)~(e)の手順に従って調理した。各材料の使用量は表6に記載の通りである。なお、本実施例においても、食品の総質量は茹でたパスタの質量を220gとして計算した。The pasta dishes were cooked according to the following steps (a) to (e). The amounts of each ingredient used are as shown in Table 6. Note that in this example, the total mass of the food was calculated based on the mass of boiled pasta being 220 g.

Figure 0007569317000006
Figure 0007569317000006

(a)表6に記載の第1の油脂組成物又は第1の混合油脂をフライパンに添加した。
(b)(a)のフライパンにニンニクを加え、IHヒーターを用いて温度強度3(100~120℃)で10分間炒め、室温まで自然冷却した。
(c)(b)のフライパンに、表6に記載の第2の油脂組成物又は第2の混合油脂と食塩とを加えて混ぜ合わせた。
(d)パスタ乾麺を水で7分間茹で、湯切りした。
(e)(c)のフライパンに(d)で茹でたパスタを加え、混ぜ合わせて実施例4-1,4-2及び比較例4-1のパスタ料理を得た。
(a) The first oil/fat composition or the first mixed oil/fat described in Table 6 was added to a frying pan.
(b) Garlic was added to the frying pan (a) and fried for 10 minutes at temperature intensity 3 (100-120°C) using an induction heater, and then naturally cooled to room temperature.
(c) The second oil and fat composition or the second mixed oil and fat described in Table 6 and salt were added to the frying pan (b) and mixed.
(d) Dried pasta was boiled in water for 7 minutes and then drained.
(e) The pasta boiled in (d) was added to the frying pan in (c) and mixed to obtain the pasta dishes of Examples 4-1 and 4-2 and Comparative Example 4-1.

得られた実施例4-1及び4-2のパスタ料理について、比較例4-1のパスタ料理(対照)との比較により塩味を官能評価した。評価は、2名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、2名の官能評価値の平均値を求めた。結果を表7に示す。The obtained pasta dishes of Examples 4-1 and 4-2 were subjected to a sensory evaluation of saltiness in comparison with the pasta dish of Comparative Example 4-1 (control). The evaluation was performed by two expert panelists according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the two panelists was calculated. The results are shown in Table 7.

Figure 0007569317000007
Figure 0007569317000007

表7に示したように、調理中に加水分解物に加熱処理を施した実施例4-1のパスタ料理及び加水分解物に加熱処理を施していない実施例4-2のパスタ料理はともに、加水分解物を含んでいない比較例4-1のパスタ料理よりも塩味が増強されていた。また、実施例4-1と実施例4-2との対比から、加水分解物に加熱処理を施すと塩味増強効果が高くなることが分かった。As shown in Table 7, the pasta dish of Example 4-1, in which the hydrolysate was heat-treated during cooking, and the pasta dish of Example 4-2, in which the hydrolysate was not heat-treated, both had a saltier taste than the pasta dish of Comparative Example 4-1, which did not contain a hydrolysate. In addition, a comparison between Example 4-1 and Example 4-2 revealed that the saltiness-enhancing effect was greater when the hydrolysate was heat-treated.

<実施例5>
本実施例では、実施例1-1の加水分解物を含ませた炒飯の塩味について評価した。
Example 5
In this example, the saltiness of fried rice containing the hydrolysate of Example 1-1 was evaluated.

実施例5-1及び比較例5-1の炒飯は、表8に記載の材料を準備して、以下の(a)~(e)の手順に従って調理した。なお、食品中の食塩は、中華スープの素5g当たりの食塩相当量を2.0gとして計算した。The fried rice in Example 5-1 and Comparative Example 5-1 was prepared using the ingredients listed in Table 8 and cooked according to the following procedures (a) to (e). The salt content of the food was calculated based on 2.0 g of salt per 5 g of Chinese soup base.

Figure 0007569317000008
Figure 0007569317000008

(a)表8に記載の油脂組成物又は混合油脂を用意してフライパンに引き、IHヒーターを用いて温度強度5で油脂組成物又は混合油脂が200℃に達するまで加熱した。
(b)10秒後に、(a)のフライパンに、溶き卵及び600Wの電子レンジで2分間温めた米飯をこの順に加えた。
(c)60秒間混ぜながら加熱した。
(d)(c)のフライパンに長ネギを加え、30秒間炒めた。
(e)(d)のフライパンに、食塩,こしょう及び中華スープの素を加えて80秒間炒め、実施例5-1及び比較例5-1の炒飯を得た。
(a) An oil composition or mixed oil described in Table 8 was prepared and placed in a frying pan, and heated at temperature intensity 5 using an IH heater until the oil composition or mixed oil reached 200°C.
(b) After 10 seconds, beaten egg and cooked rice that had been heated in a 600 W microwave for 2 minutes were added, in that order, to the frying pan in (a).
(c) Heat with mixing for 60 seconds.
(d) Add green onions to the frying pan (c) and stir fry for 30 seconds.
(e) Salt, pepper and Chinese soup stock were added to the frying pan (d) and fried for 80 seconds to obtain the fried rice of Example 5-1 and Comparative Example 5-1.

得られた実施例5-1の炒飯について、比較例5-1の炒飯(対照)との比較により塩味を官能評価した。評価は、2名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、2名の官能評価値の平均値を求めた。結果を表9に示す。The fried rice of Example 5-1 obtained was subjected to a sensory evaluation of saltiness in comparison with the fried rice of Comparative Example 5-1 (control). The evaluation was performed by two expert panelists according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the two panelists was calculated. The results are shown in Table 9.

Figure 0007569317000009
Figure 0007569317000009

表9に示したように、本発明の加水分解物を含ませた実施例5-1の炒飯は、加水分解物を含ませていない比較例5-1の炒飯に比べて塩味が増強されていた。As shown in Table 9, the fried rice of Example 5-1, which contained the hydrolysate of the present invention, had an enhanced saltiness compared to the fried rice of Comparison Example 5-1, which did not contain the hydrolysate.

<実施例6>
本実施例では、実施例1-1の加水分解物を含ませた炊き込みご飯の塩味について評価した。
Example 6
In this example, the saltiness of cooked rice containing the hydrolysate of Example 1-1 was evaluated.

実施例6-1,6-2及び比較例6-1の炊き込みご飯は、表10に記載の材料を準備して、以下の(a)~(e)の手順に従って調理した。なお、食品中の食塩含有量は、しょう油15mL(18g)当たりの食塩相当量を2.4g、酒15mL(15g)当たりの食塩相当量を0.37gとして計算した。また、食品の総質量は、炊きあがった後の炊き込みご飯の質量を900gとして計算した。The cooked rice of Examples 6-1 and 6-2 and Comparative Example 6-1 was prepared using the ingredients listed in Table 10 and cooked according to the following procedures (a) to (e). The salt content in the food was calculated based on 2.4 g of salt per 15 mL (18 g) of soy sauce and 0.37 g of salt per 15 mL (15 g) of sake. The total mass of the food was calculated based on 900 g of the mass of cooked cooked rice.

Figure 0007569317000010
Figure 0007569317000010

(a)生米を洗い、炊飯器(「圧力IH炊飯ジャーNP-NT10」、象印マホービン株式会社製)の内釜に入れた。
(b)(a)の内釜に、表10に記載の調味料と鶏モモ肉とを加え、生米,調味料,鶏モモ肉及び水の合計質量が972gとなるように水を加えた。
(c)(b)の内釜に、表10に記載の油脂組成物又は菜種油と乳化剤とを加えた。
(d)(c)の内釜を炊飯器にセットし、「ふつう」モードで炊飯を開始した。
(e)炊飯完了後、米飯をほぐし、真空冷却器(「CMJ-20QE」、三浦工業株式会社製)にて23℃まで冷却し、実施例6-1,6-2及び比較例6-1の炊き込みご飯を得た。
(a) Raw rice was washed and placed in the inner pot of a rice cooker ("Pressure IH Rice Cooker NP-NT10", manufactured by Zojirushi Corporation).
(b) The seasonings and chicken thigh meat shown in Table 10 were added to the inner pot of (a), and water was added so that the total mass of the raw rice, seasonings, chicken thigh meat, and water was 972 g.
(c) The oil or fat composition shown in Table 10 or rapeseed oil and an emulsifier were added to the inner pot of (b).
(d) The inner pot of (c) was placed in a rice cooker, and rice cooking was started in the "normal" mode.
(e) After the rice cooking was completed, the cooked rice was loosened and cooled to 23°C in a vacuum cooler ("CMJ-20QE", manufactured by Miura Industrial Co., Ltd.) to obtain the cooked rice of Examples 6-1, 6-2 and Comparative Example 6-1.

得られた実施例6-1及び6-2の炊き込みご飯について、比較例6-1の炊き込みご飯(対照)との比較により塩味を官能評価した。評価は、3名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、3名の官能評価値の平均値を求めた。結果を表11に示す。The resulting cooked rice of Examples 6-1 and 6-2 was subjected to a sensory evaluation of saltiness in comparison with the cooked rice of Comparative Example 6-1 (control). The evaluation was performed by a panel of three experts according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the three experts was calculated. The results are shown in Table 11.

Figure 0007569317000011
Figure 0007569317000011

表11に示したように、本発明の加水分解物を含ませた実施例6-1及び6-2の炊き込みご飯は、加水分解物を含ませていない比較例6-1の炊き込みご飯に比べて塩味が増強されていた。As shown in Table 11, the cooked rice of Examples 6-1 and 6-2, which contained the hydrolysate of the present invention, had an enhanced saltiness compared to the cooked rice of Comparison Example 6-1, which did not contain the hydrolysate.

<実施例7>
本実施例では、実施例1-1の加水分解物を含ませた野菜炒めの塩味について評価した。
Example 7
In this example, the saltiness of stir-fried vegetables containing the hydrolysate of Example 1-1 was evaluated.

実施例7-1及び比較例7-1,7-2の野菜炒めは、表12に記載の材料を準備して、以下の(a)~(c)の手順に従って調理した。The stir-fried vegetables of Example 7-1 and Comparative Examples 7-1 and 7-2 were prepared by preparing the ingredients listed in Table 12 and cooking according to the steps (a) to (c) below.

Figure 0007569317000012
Figure 0007569317000012

(a)フライパンに表12に記載の油脂組成物又は菜種油を引き、IHヒーターの温度強度5で油脂組成物又は菜種油の温度が200℃に達するまで加熱した。
(b)30秒後に、(a)のフライパンに野菜を加え、混ぜ合わせながら2分間加熱した。
(c)(b)のフライパンに食塩を加えて1分間炒め、実施例7-1及び比較例7-1,7-2の野菜炒めを得た。
(a) The oil or fat composition shown in Table 12 or rapeseed oil was poured into a frying pan, and heated at temperature intensity 5 of an IH heater until the temperature of the oil or fat composition or rapeseed oil reached 200°C.
(b) After 30 seconds, the vegetables were added to the frying pan in (a) and heated for 2 minutes while mixing.
(c) Salt was added to the frying pan (b) and the vegetables were fried for 1 minute to obtain stir-fried vegetables of Example 7-1 and Comparative Examples 7-1 and 7-2.

得られた実施例7-1及び比較例7-2の野菜炒めについて、比較例7-1の野菜炒め(対照)との比較により塩味を官能評価した。評価は、3名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、3名の官能評価値の平均値を求めた。結果を表13に示す。The resulting stir-fried vegetables of Example 7-1 and Comparative Example 7-2 were subjected to a sensory evaluation of saltiness in comparison with the stir-fried vegetables of Comparative Example 7-1 (control). The evaluation was performed by a panel of three experts according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the three experts was calculated. The results are shown in Table 13.

Figure 0007569317000013
Figure 0007569317000013

表13に示したように、本発明の加水分解物を含ませた実施例7-1の野菜炒めは、加水分解物を含ませていない比較例7-1の野菜炒めに比べて塩味が増強されていた。また、実施例7-1の野菜炒めは、含まれる食塩の割合が同じである比較例7-1の野菜炒めの評価値(0)よりも、食塩の割合が高い比較例7-2の野菜炒めの評価値(+6)に近い評価値が得られた。例えば、線形補間で近似すると、実施例7-1の野菜炒めは、本発明の加水分解物を含ませていない、食品中の食塩の割合が0.58質量%相当の塩味と同等であると考えられる。As shown in Table 13, the stir-fried vegetables of Example 7-1, which contained the hydrolysate of the present invention, had an enhanced saltiness compared to the stir-fried vegetables of Comparative Example 7-1, which did not contain the hydrolysate. Furthermore, the stir-fried vegetables of Example 7-1 obtained an evaluation value closer to the evaluation value (+6) of the stir-fried vegetables of Comparative Example 7-2, which contained a higher proportion of salt, than the evaluation value (0) of the stir-fried vegetables of Comparative Example 7-1, which contained the same proportion of salt. For example, when approximating by linear interpolation, the stir-fried vegetables of Example 7-1 is considered to be equivalent to the saltiness of a food that does not contain the hydrolysate of the present invention and has a salt content of 0.58% by mass.

<実施例8>
本実施例においては、実施例1-1の加水分解物を含ませたスープの塩味について評価した。
Example 8
In this example, the saltiness of soup containing the hydrolysate of Example 1-1 was evaluated.

実施例8-1及び比較例8-1,8-2のスープは、表14に記載の材料を準備して、以下の(a)及び(b)の手順に従って調理した。なお、食品中の食塩含有量は、スープの素2.5g当たりの食塩相当量を1.2gとして計算した。The soups of Example 8-1 and Comparative Examples 8-1 and 8-2 were prepared by preparing the ingredients listed in Table 14 and cooking according to the procedures (a) and (b) below. The salt content in the food was calculated based on 1.2 g of salt per 2.5 g of soup base.

Figure 0007569317000014
Figure 0007569317000014

(a)鍋に水を入れた後、スープの素を入れ、IHヒーターを用いて加熱した。比較例8-2については、スープの素とともに食塩を入れた。
(b)沸騰後、更に5分間加熱し、実施例8-1及び比較例8-1,8-2のスープを得た。
(a) After pouring water into a pot, the soup base was added and heated using an induction heater. For Comparative Example 8-2, salt was added together with the soup base.
(b) After boiling, the mixture was heated for an additional 5 minutes to obtain the soups of Example 8-1 and Comparative Examples 8-1 and 8-2.

得られた実施例8-1及び比較例8-2のスープについて、比較例8-1のスープ(対照)との比較により塩味を官能評価した。評価は、2名の専門パネルにより、上記実施例2の評価で説明した採点基準に従って行い、2名の官能評価値の平均値を求めた。結果を表15に示す。The resulting soups of Example 8-1 and Comparative Example 8-2 were subjected to a sensory evaluation of saltiness in comparison with the soup of Comparative Example 8-1 (control). The evaluation was performed by two expert panelists according to the scoring criteria explained in the evaluation of Example 2 above, and the average of the sensory evaluation values of the two panelists was calculated. The results are shown in Table 15.

Figure 0007569317000015
Figure 0007569317000015

表15から分かるように、本発明の加水分解物を含ませた実施例8-1のスープは、加水分解物を含ませていない比較例8-1のスープに比べて塩味が増強されていた。また、実施例8-1のスープは、食品中の食塩の割合が0.63質量%である比較例8-1のスープと、0.78質量%である比較例8-2のスープとの間の評価値が得られ、線形補間で近似すると、本発明の加水分解物を含ませていない、食品中の食塩の割合が0.71質量%相当の塩味と同等であると考えられる。
As can be seen from Table 15, the soup of Example 8-1 containing the hydrolysate of the present invention had an enhanced saltiness compared to the soup of Comparative Example 8-1 not containing the hydrolysate. Furthermore, the soup of Example 8-1 obtained an evaluation value between the soup of Comparative Example 8-1, in which the salt content in the food was 0.63 mass%, and the soup of Comparative Example 8-2, in which the salt content in the food was 0.78 mass%, and when approximated by linear interpolation, it is considered to be equivalent to the saltiness of a food not containing the hydrolysate of the present invention and in which the salt content in the food was 0.71 mass%.

Claims (22)

全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を有効成分とする、食品の塩味増強剤。 A food saltiness enhancer whose active ingredient is a hydrolysate of edible oils and fats containing linoleic acid and oleic acid at a total content of 30% by mass or more of total fatty acids. 前記リノール酸及びオレイン酸の合計が70質量%以上である、請求項1に記載の食品の塩味増強剤。 The salty taste enhancer for foods according to claim 1 , wherein the total content of the linoleic acid and the oleic acid is 70% by mass or more. 前記全脂肪酸中に含まれる前記オレイン酸が15質量%以上80質量%以下である、請求項1又は2に記載の食品の塩味増強剤。 The salty taste enhancer for food according to claim 1 or 2, wherein the oleic acid contained in the total fatty acids is 15% by mass or more and 80% by mass or less. 前記食用油脂は、大豆油、菜種油、コーン油及びオリーブ油から選ばれる一種又は二種以上である、請求項1乃至3のいずれか1項に記載の食品の塩味増強剤。 The salty taste enhancer for food according to any one of claims 1 to 3, wherein the edible oil is one or more selected from the group consisting of soybean oil, rapeseed oil, corn oil and olive oil. 前記加水分解物はリパーゼ処理物である、請求項1乃至4のいずれか1項に記載の食品の塩味増強剤。 The salty taste enhancer for food according to any one of claims 1 to 4, wherein the hydrolysate is a lipase-treated product. 前記リパーゼが微生物由来である、請求項5に記載の食品の塩味増強剤。 The salty taste enhancer for food according to claim 5, wherein the lipase is derived from a microorganism. 前記加水分解物は加熱処理されたものである、請求項1乃至6のいずれか1項に記載の食品の塩味増強剤。 The salty taste enhancer for food according to any one of claims 1 to 6, wherein the hydrolysate is heat-treated. 全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物を食材に含ませることを特徴とする、食品の塩味増強方法。 A method for enhancing the saltiness of food, comprising adding to food ingredients a hydrolysate of edible oils and fats in which the total amount of linoleic acid and oleic acid in the total fatty acids is 30% by mass or more. 全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物であって、脂肪酸の加水分解による分離率が85%以上100%以下であるものを0.01質量%以上20質量%以下含む油脂組成物であり、前記食用油脂の加水分解物が加熱処理されているか、あるいは前記油脂組成物が食材と共に加熱処理されるものである食塩又は塩味を感じる成分を含む食品に適用するための油脂組成物。 An oil and fat composition for application to foods containing salt or ingredients that have a salty taste, the oil and fat composition comprising a hydrolysate of an edible oil and fat containing 30% by mass or more of linoleic acid and oleic acid in total fatty acids, and containing 0.01% by mass or more and 20% by mass or less of fatty acids having a separation rate by hydrolysis of 85% to 100%, the hydrolysate of the edible oil and fat being heat-treated, or the oil and fat composition being heat-treated together with food ingredients . 前記リノール酸及びオレイン酸の合計が70質量%以上である、請求項9に記載の油脂組成物。 The oil and fat composition according to claim 9, wherein the total content of the linoleic acid and the oleic acid is 70 mass% or more. 前記全脂肪酸中に含まれるオレイン酸が15質量%以上80質量%以下である、請求項9又は10に記載の油脂組成物。 The oil composition according to claim 9 or 10, wherein the amount of oleic acid contained in the total fatty acids is 15% by mass or more and 80% by mass or less. 前記食用油脂は、大豆油、菜種油、コーン油及びオリーブ油から選ばれる一種又は二種以上である、請求項9乃至11のいずれか1項に記載の油脂組成物。 The oil and fat composition according to any one of claims 9 to 11, wherein the edible oil and fat is one or more selected from soybean oil, rapeseed oil, corn oil, and olive oil. 前記加水分解物はリパーゼ処理物である、請求項9乃至12のいずれか1項に記載の油脂組成物。 The oil composition according to any one of claims 9 to 12, wherein the hydrolysate is a lipase-treated product. 前記リパーゼが微生物由来である、請求項13に記載の油脂組成物。 The oil composition according to claim 13, wherein the lipase is derived from a microorganism. 前記油脂組成物は、炒め用及び炊飯用から選ばれる一種又は二種である、請求項9乃至14のいずれか1項に記載の油脂組成物。 The oil and fat composition according to any one of claims 9 to 14 , wherein the oil and fat composition is one or two types selected from oil and fat compositions for frying and oil and fat compositions for rice cooking. 全脂肪酸中にリノール酸及びオレイン酸の合計が30質量%以上含まれる食用油脂の加水分解物であって、脂肪酸の加水分解による分離率が85%以上100%以下であるものを、予め加熱処理して、食塩又は塩味を感じる成分を含む食品に0.0001質量%以上1質量%以下含ませるか、あるいは、前記食用油脂の加水分解物を、前記食品に0.0001質量%以上1質量%以下含ませた後、加熱処理することを特徴とする、食品の製造方法。 A method for producing a food, comprising: preliminarily heat-treating a hydrolysate of an edible oil or fat, the total of which contains linoleic acid and oleic acid at 30% by mass or more in total fatty acids, and the separation rate of the fatty acids by hydrolysis is 85% to 100%, and then incorporating the hydrolysate in a food containing salt or a component that has a salty taste at 0.0001% by mass or more and 1% by mass or less; or incorporating the hydrolysate of the edible oil or fat in a food at 0.0001% by mass or more and 1% by mass or less, and then heat-treating the food. 前記リノール酸及びオレイン酸の合計が70質量%以上である、請求項16に記載の食品の製造方法。 The method for producing a food product according to claim 16 , wherein the total content of linoleic acid and oleic acid is 70% by mass or more. 前記全脂肪酸中に含まれるオレイン酸が15質量%以上80質量%以下である、請求項16又は17に記載の食品の製造方法。 The method for producing a food product according to claim 16 or 17 , wherein the content of oleic acid in the total fatty acids is 15% by mass or more and 80% by mass or less. 前記食用油脂は、大豆油、菜種油、コーン油及びオリーブ油から選ばれる一種又は二種以上である、請求項16乃至18のいずれか1項に記載の食品の製造方法。 The method for producing a food product according to any one of claims 16 to 18 , wherein the edible oil or fat is one or more selected from the group consisting of soybean oil, rapeseed oil, corn oil and olive oil. 前記加水分解物はリパーゼ処理物である、請求項16乃至19のいずれか1項に記載の食品の製造方法。 The method for producing a food product according to any one of claims 16 to 19 , wherein the hydrolysate is a lipase-treated product. 前記リパーゼが微生物由来である、請求項20に記載の食品の製造方法。 The method of claim 20 , wherein the lipase is derived from a microorganism. 前記加水分解物を、前記食品に含ませる前に加熱処理する、請求項16乃至21のいずれか1項に記載の食品の製造方法。 22. A method for producing a food product according to any one of claims 16 to 21 , wherein the hydrolysate is heat treated prior to inclusion in the food product .
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