JP7600398B2 - Efficacy enhancing compositions and uses thereof - Google Patents

Description

The present invention is in the field of biopharmaceuticals, and more specifically, relates to efficacy enhancing compositions and uses thereof.

The prerequisite for a cosmetic product to achieve maximum efficacy is that the active ingredients have good penetration and activity, and that the utilization rate of the active ingredients is increased.

Transdermal absorption of cosmetics refers to the process in which functional ingredients in cosmetics act on the skin surface or penetrate the epidermis or dermis depending on the effectiveness of the product, accumulating at that site and acting. The main difference between transdermal absorption of cosmetic active ingredients and drugs is that they do not need to penetrate the skin to enter the systemic circulation. For example, whitening agents in whitening products act on the basal layer of the skin to block melanin production, active ingredients in anti-aging products act on fibroblasts in the dermis layer to give the skin firmness, sunscreen agents in sunscreen products remain on the surface of the skin to absorb and reflect ultraviolet rays, while some moisturizing products act on the epidermis or dermis layer through different mechanisms of action to improve the skin's moisture content.

There are many factors that affect the percutaneous absorption of cosmetics, mainly skin factors, the structure of the active ingredient, substance concentration, cosmetic formulation, and external environment. Currently, it is common on the market to increase the permeability of cosmetics by selecting active ingredients similar to those on the skin or changing the cosmetic formulation, but the effect is uniform and there are certain limitations to the penetration-enhancing effect.

Therefore, there is an urgent need for an efficacy-promoting composition that can promote the penetration and absorption of active ingredients according to the location where each active ingredient exerts its effect, achieve accurate localization of the active ingredients, and rapidly exert skin care effects, while also improving the stability of the active ingredients, increasing the contact time of the active ingredients with the skin, and improving the utilization rate of the active ingredients.

The present invention aims to provide an efficacy-enhancing composition that can effectively improve the penetration and absorption of active ingredients and the stability of the active ingredients while providing moisturizing and hydration, thereby improving the utilization rate of the active ingredients and improving skin conditions.

Specifically, the present invention relates to the following aspects.
1. An efficacy-enhancing composition comprising high molecular weight hyaluronic acid or its salt, hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt, tetrahydropyrimidine or its derivative, ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid.
2. The composition according to item 1, characterized in that the polymeric hyaluronic acid or a salt thereof is 1 to 30% by mass, preferably 1 to 10% by mass, the hydrolyzed hyaluronic acid or a salt thereof is 2% by mass to 50% by mass, preferably 2 to 10% by mass, the acetylated hyaluronic acid or a salt thereof is 1 to 10% by mass, preferably 1 to 1.5% by mass, the tetrahydropyrimidine or a derivative thereof is 0.1% by mass to 10% by mass, preferably 0.3 to 5% by mass, the ergothioneine is 2 to 20% by mass, preferably 3 to 10% by mass, the Saccharomyces/rice fermentation liquor is 10 to 50% by mass, preferably 15 to 40% by mass, and the aminobutyric acid is 0.1 to 5% by mass, preferably 0.3 to 3% by mass.
3. The composition according to item 1 or 2, characterized in that, on a part by weight basis, the polymeric hyaluronic acid or its salt is 1 part by weight, the hydrolyzed hyaluronic acid or its salt is 0.2 to 2 parts by weight, preferably 0.5 to 1 part by weight, and the acetylated hyaluronic acid or its salt is 0.05 to 1.5 parts by weight, preferably 0.1 to 0.5 parts by weight.
4. The composition according to any one of Items 1 to 3, wherein the molecular weight of the high molecular weight hyaluronic acid or its salt is 1000 to 1800 kDa, preferably 1000 to 1500 kDa.
5. The composition according to any one of items 1 to 4, wherein the molecular weight of the hydrolyzed hyaluronic acid is 1 kDa to 10 kDa, preferably 5 kDa to 10 kDa.
6. The composition according to any one of items 1 to 5, wherein the molecular weight of the acetylated hyaluronic acid or a salt thereof is 20 kDa to 100 kDa, and the acetyl content is 20 to 40 mass %.
7. The composition according to any one of items 1 to 6, wherein the tetrahydropyrimidine or a derivative thereof is one or two selected from ectoine and hydroxyectoine , preferably ectoine .
8. The composition according to any one of Items 1 to 7, further comprising a preservative, the preservative being 0.1 to 5% by mass of one or more preservatives selected from the group consisting of pentylene glycol, hexylene glycol, butylene glycol, and ethylhexylglycerin.
9. Use of the composition according to any one of items 1 to 8 in skin care products or cosmetics.
10. A skin care product or cosmetic comprising the composition according to any one of items 1 to 8.

The present invention has the following beneficial effects:
1. Acetylated hyaluronic acid is made by combining high molecular weight hyaluronic acid with hydrolyzed hyaluronic acid of a specific molecular weight in a specific mixing ratio to construct a stable "工"-shaped channel in three-dimensional space, forming a long-term hydration channel from the moisturizing and moisture-retaining layer on the skin surface to the hydration layer deep in the skin, thereby effectively hydrating and increasing the moisture content of the skin.
2. The synergistic effect of the four components, tetrahydropyrimidine, ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid, ensures the efficient operation of the "工"-shaped structure formed by sodium hyaluronate and its derivatives, thereby achieving more efficient repair of the skin barrier, repair of damage, and improvement of skin surface condition.
3. The "工"-shaped efficacy promotion system constructed by seven components not only hydrates and repairs the skin, but also enhances the stability of high molecular weight active ingredients and increases the contact time of the active ingredients with the skin, thereby improving the utilization rate of the high molecular weight active ingredients, reducing the amount of active ingredients used, saving costs, and reducing the skin irritation caused by excessive use of some active ingredients. Meanwhile, the "工"-shaped channel can increase the transdermal absorption effect of small molecule active ingredients and increase the utilization rate of small molecule active ingredients.

The present invention will be further described below with reference to examples. However, it should be understood that the examples are used only to further describe the present invention and are not used to limit the present invention.

Unless otherwise defined, technical and scientific terms herein have the same meaning as commonly understood by one of ordinary skill in the art. Methods and materials similar or identical to those described herein may be applied in experimental or practical applications, but the materials and methods are described below. In case of conflict, the definitions contained herein shall prevail, and the materials, methods and examples are merely illustrative and not limiting. The present invention is further described below with reference to specific examples, but not intended to limit the scope of the present invention.

The present invention provides an efficacy-enhancing composition comprising high molecular weight hyaluronic acid or a salt thereof, hydrolyzed hyaluronic acid or a salt thereof, acetylated hyaluronic acid or a salt thereof, tetrahydropyrimidine or a derivative thereof, ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid.

In one specific embodiment, the polymeric hyaluronic acid or its salt may be 1-30% by weight, for example, 1%, 5%, 10%, 15%, 20%, 25%, 30%, preferably 1-10% by weight. The hydrolyzed hyaluronic acid or its salt may be 2%-50% by weight, for example, 2%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, preferably 2-10% by weight, and the acetylated hyaluronic acid or its salt may be 1-10% by weight, for example, 1%, 1.2%, 1.5%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, preferably 1-1.5% by weight. The tetrahydropyrimidine or derivative thereof may be 0.1% by mass to 10% by mass, for example, 0.1%, 0.3%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, and preferably 0.3% to 5% by mass. The ergothioneine may be 2% to 20% by mass, 2%, 3%, 5%, 10%, 15%, 20%, and preferably 3% to 10% by mass. The Saccharomyces/rice fermentation liquor may be 10% to 50% by mass, for example, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, and preferably 15% to 40% by mass. The aminobutyric acid may be 0.1 to 5% by weight, for example 0.1%, 0.3%, 0.5%, 1%, 2%, 3%, 4%, 5% by weight, preferably 0.3 to 3% by weight.

Hyaluronic acid (HA) is a polymeric polysaccharide that naturally exists in the interstitium of human tissue cells and is widely used in the fields of medicine, skin care products, food, etc. It has various molecular weights, and HA of each molecular weight has different efficacy and expression. High molecular weight HA has excellent film-forming and moisture-retaining properties, low molecular weight hydrolyzed HA can penetrate the skin and deeply hydrate the skin, and acetylated HA can function as a bridge between the skin surface and hydrolyzed HA, and is a natural and effective moisturizing and moisture-retaining factor.

By combining three different types of HA, a surface moisturizing and moisture-retaining layer made up mainly of polymeric HA is formed, a deep hydration layer made up mainly of hydrolyzed HA, and a moisture transport layer made of acetylated HA that connects the two parts is formed in space, creating a "工"-shaped long-term hydration and moisture-retaining channel in a three-dimensional space.

In one specific embodiment, the molecular weight of the high molecular weight hyaluronic acid or its salt may be 1000-1800 kDa, for example 1000 kDa, 1100 kDa, 1200 kDa, 1300 kDa, 1400 kDa, 1500 kDa, 1600 kDa, 1700 kDa, 1800 kDa, preferably 1000-1500 kDa. The molecular weight of the hydrolyzed hyaluronic acid or its salt may be 1 kDa-10 kDa, for example 1 kDa, 2 kDa, 3 kDa, 4 kDa, 5 kDa, 6 kDa, 7 kDa, 8 kDa, 9 kDa, 10 kDa, preferably 5 kDa-10 kDa. The molecular weight of the acetylated hyaluronic acid or its salt may be 20 kDa to 100 kDa, for example, 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 70 kDa, 80 kDa, 90 kDa, or 100 kDa. The acetyl group in the acetylated hyaluronic acid or its salt may be 20 to 40% by mass, for example, 20% by mass, 25% by mass, 30% by mass, 35% by mass, or 40% by mass.

In one specific embodiment, the polymeric hyaluronic acid or its salt is 1 part by weight, and the hydrolyzed hyaluronic acid or its salt is 0.2 to 2 parts by weight, for example, 0.2 parts by weight, 0.3 parts by weight, 0.4 parts by weight, 0.5 parts by weight, 0.6 parts by weight, 0.7 parts by weight, 0.8 parts by weight, 0.9 parts by weight, 1 parts by weight, 1.1 parts by weight, 1.2 parts by weight, 1.3 parts by weight, 1.4 parts by weight, 1.5 parts by weight, 1.6 parts by weight, 1.7 parts by weight, 1.8 parts by weight, 1.9 ...9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1.9 parts by weight, 1. .8 parts by weight, 1.9 parts by weight, 2 parts by weight, preferably 0.5 to 1 part by weight, and the acetylated hyaluronic acid or its salt may be 0.05 to 1.5 parts by weight, for example, 0.05 parts by weight, 0.1 parts by weight, 0.2 parts by weight, 0.3 parts by weight, 0.4 parts by weight, 0.5 parts by weight, 1 part by weight, 1.1 parts by weight, 1.2 parts by weight, 1.3 parts by weight, 1.4 parts by weight, 1.5 parts by weight, preferably 0.1 to 0.5 parts by weight.

Ectoine is the most widely compatible solute currently found in the bacterial kingdom, and is an important osmotic compensation solute that is compatible with intracellular metabolism and does not affect the function of biopolymers or physiological functions of cells. Due to the dense charge distribution on the surface of ectoine, it can further strengthen the hydrogen bond between water molecules through electrostatic interaction with water molecules, reduce water activity, complex water molecules, and have a long-term moisturizing effect. Through its own high-density charge, it can strengthen the hydrogen bond between water molecules and moisturize for a long time, and ectoine has excellent hydrophilicity, so that the above-mentioned C-shaped structure can more efficiently repair the skin barrier and repair damage.

In one specific embodiment, the tetrahydropyrimidine or derivative thereof is one or two selected from ectoine and hydroxyectoine , preferably ectoine .

Ergothioneine is a natural antioxidant that is distributed in certain tissues and organs of mammals and can be produced by fermentation. It has a protective effect on cells in the human body and has a UV protection effect, making it an important active substance in the body. Through synergistic effects with ECTOIN, it can effectively protect the skin barrier and ensure the function of the "U"-shaped structure formed by sodium hyaluronate and its derivatives.

Saccharomyces/ Rice Ferment Filtrate is a natural ingredient produced by fermentation, and has the effect of increasing cell vitality and promoting cell regeneration. It can effectively penetrate the thin film formed on the skin surface by high molecular weight HA, and remain on the skin surface for a long time, promoting the regeneration of surface damaged cells, and through a synergistic effect with ergothioneine and ectoin, it can more efficiently improve the condition of the skin surface and increase its moisturizing ability.

Aminobutyric acid promotes the proliferation of epidermal cells and the production of involucrin in keratinocytes, repairing the barrier of the stratum corneum, reducing skin damage caused by ultraviolet rays, and enhancing cell activity.

Tetrahydropyrimidine or its derivatives, ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid effectively protect the skin barrier and ensure the stability and durability of the film formed on the skin surface by high molecular weight HA, thereby ensuring the efficient operation of the "U"-shaped structure formed by sodium hyaluronate and its derivatives.

The composition further contains a preservative, and the preservative is one or more selected from pentylene glycol, hexylene glycol, butylene glycol, and ethylhexylglycerin, and the preservative is present in an amount of 0.1 to 5% by mass in the composition.

The present invention also provides an efficacy enhancing composition comprising high molecular weight hyaluronic acid or its salt, hydrolyzed hyaluronic acid or its salt, acetylated hyaluronic acid or its salt, tetrahydropyrimidine or its derivative, ergothioneine, Saccharomyces/rice fermentation liquid , aminobutyric acid, a preservative and water, the mass ratio of each component and the properties such as molecular weight are as described above.

The present invention also provides the use of the above composition in skin care products or cosmetics.

The efficacy-promoting composition of the present invention contains high molecular weight hyaluronic acid, hydrolyzed hyaluronic acid, acetylated hyaluronic acid, tetrahydropyrimidine, ergothioneine, Saccharomyces/rice fermentation liquid and aminobutyric acid in a specific mixing ratio, and thus has an effective efficacy-promoting function.For example, it can significantly promote hydration, improve the stability of active ingredients, and improve the absorption of anti-aging ingredients.This is probably because acetylated hyaluronic acid connects high molecular weight hyaluronic acid with hydrolyzed hyaluronic acid of a specific molecular weight in a specific mixing ratio, constructs a stable "W"-shaped channel in three-dimensional space, while the synergistic effect of tetrahydropyrimidine, ergothioneine, Saccharomyces/rice fermentation liquid and aminobutyric acid ensures the efficient operation of the "W"-shaped structure formed by sodium hyaluronate and its derivatives.
Working Example

The present invention will be further described below with reference to examples. However, it should be understood that the examples are used only to further describe the present invention and are not used to limit the present invention.

The experimental methods used in the following examples are conventional methods unless otherwise specified.
Materials, reagents, etc. used in the following examples are commercially available unless otherwise specified. Among them, high molecular weight sodium hyaluronate, hydrolyzed sodium hyaluronate, acetylated sodium hyaluronate, ectoine , ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid are all from Bloomage Biotechnology corporation limited.

Example 1
2.5g of high molecular weight sodium hyaluronate (1200kDa) is added to an appropriate amount of water, and stirred until completely dissolved.Then, 2.5g of hydrolyzed sodium hyaluronate (5kDa) and 1g of acetylated sodium hyaluronate (molecular weight 4kDa, acetyl content 24%) are added, and stirred uniformly until completely dissolved.3g of ectoine , 7g of ergothioneine, 20g of Saccharomyces/rice fermentation liquid , 1.5g of aminobutyric acid, and 5g of preservatives (2.5g of pentanediol, 2.5g of hexanediol) are added in order, and water is replenished to 100g, and stirred uniformly until completely dissolved, to obtain an efficacy-promoting composition.

Compared with Example 1, Examples 2 to 13 and Comparative Examples 1 to 8 have different molecular weights or contents of high molecular weight sodium hyaluronate, hydrolyzed sodium hyaluronate, and acetylated sodium hyaluronate, as well as different contents of ectoine , ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid, as shown in Table 1. Among them, the difference between Example 9 and Example 1 is only the difference in the molecular weight of high molecular weight sodium hyaluronate, the difference between Example 10 and Example 1 is only the difference in the content of high molecular weight sodium hyaluronate, the difference between Example 11 and Example 1 is only the difference in the content of hydrolyzed sodium hyaluronate, the difference between Example 12 and Example 1 is only the difference in the molecular weight of hydrolyzed sodium hyaluronate, and the difference between Example 13 and Example 1 is the difference in the content of acetylated sodium hyaluronate.

Test Example 1 Hydration Effect 1. The skin moisture content was measured in 22 different areas (area numbers 1 to 22) of the forearm, and the initial value data was recorded.
2. 1% by mass of ceramide-3 was added to each of the efficacy enhancing compositions prepared in Examples 1 to 13 and Comparative Examples 1 to 8 to obtain hydrating compositions corresponding to Examples 1 to 13 and Comparative Examples 1 to 8.
3. The hydrating compositions corresponding to Examples 1-13 and Comparative Examples 1-8 were applied in equal amounts to 21 different regions (region numbers 1-21) of the forearm, and after 30 minutes, the skin moisture content was measured and recorded using a skin moisture loss measuring device Tewameter TM300 (Courage+Khazaka, Germany). Among these, the hydrating composition of Example 1 corresponds to region 1, the hydrating composition of Example 2 corresponds to region 2, and thus the hydrating composition of Example 8 corresponds to region 8, the hydrating composition of Comparative Example 1 corresponds to region 14, the hydrating composition of Comparative Example 2 corresponds to region 15, and the hydrating composition of Comparative Example 8 corresponds to region 21.
4. The same amount of a hydrating ingredient solution (1% by weight of ceramide-3) was applied to area 22 as a control, and after 30 minutes, the skin moisture content was measured and recorded.
5. The number of subjects was 10, and the moisture content was the average value.

The hydration effects of each of the above hydration compositions are shown in Table 2.

Conclusion: From Table 2, it can be seen that the addition of the compositions prepared in the examples of the present invention significantly increases the hydrating ability of the hydrating ingredients, and in particular, the hydrating effect of the compositions obtained in Examples 1 to 4 is the highest, indicating that the efficacy-promoting composition of the present invention can effectively promote hydration.
In Comparative Examples 1 to 3, only two of the three hyaluronic acids are contained, and therefore the hydration effect is low. This is believed to be because the three hyaluronic acids of the present invention construct a stable "工"-shaped channel in three-dimensional space, forming a continuous hydration and moisture-retaining channel from the moisturizing and water-retaining layer on the skin surface to the deep skin hydration layer, effectively hydrating and increasing the moisture content of the skin. At the same time, the "工"-shaped channel can increase the transdermal absorption effect of ceramide-3, increase the utilization rate of ceramide-3, and enhance the hydration effect.

The hydration effect of Comparative Examples 4 to 8 was also low, and among them, the hydration effect of Comparative Example 8 was the lowest, with the difference in skin moisture before and after use being only 0.21.This is believed to be because the synergistic effect of the four components, tetrahydropyrimidine, ergothioneine, Saccharomyces/rice fermentation liquid , and aminobutyric acid, can maintain the stability and durability of the film formed by high molecular weight hyaluronic acid on the skin surface, and ensure the efficient operation of the "工"-shaped structure formed by sodium hyaluronate and its derivatives.

Test Example 2 Protective effect on active ingredient Astaxanthin was added to the efficacy enhancing compositions produced in Examples 1 to 13 and Comparative Examples 1 to 8 so that the mass% of astaxanthin in the composition was 0.01%, to obtain astaxanthin-containing compositions corresponding to Application Examples 1 to 16, respectively. Here, the astaxanthin composition obtained in Example 1 corresponds to Application Example 1, the astaxanthin composition obtained in Example 2 corresponds to Application Example 2, thus, the astaxanthin composition obtained in Example 8 corresponds to Application Example 8, the astaxanthin composition obtained in Example 13 corresponds to Application Example 13, the astaxanthin composition obtained in Comparative Example 1 corresponds to Application Example 14, the astaxanthin composition obtained in Comparative Example 2 corresponds to Application Example 15, and the astaxanthin composition obtained in Comparative Example 8 corresponds to Application Example 21.

The astaxanthin compositions of Application Examples 1 to 21 were placed in a transparent container and left at room temperature for 2 hours, during which time they were constantly stirred to expose them to oxygen, and the color change of the composition was observed. An appropriate amount of the composition was then taken and diluted with trichloromethane solvent to a solution with a concentration of 1 μg/mL, and the absorbance at 489 nm was measured using a UV8500 ultraviolet spectrophotometer. Astaxanthin stored in a light-shielded, sealed container at the same concentration was used as a control, and the absorbance at 489 nm was measured under the same conditions. The results are shown in Table 3.

Conclusion: Astaxanthin is easily oxidized and becomes lighter in color, and the lighter the color, the more easily astaxanthin is oxidized, indicating poor efficacy of astaxanthin. When the composition produced in the examples of the present invention is added, the fading of the color of astaxanthin becomes less noticeable, and in particular in Examples 1 to 4, the color change is the smallest, with the absorbance decreasing by only 0.0391 to 0.0969 compared to the control stored in a sealed, light-shielded container. However, in the comparative example, the color change of the astaxanthin composition was large, and the absorbance decreased by 0.3 or more. This shows that the efficacy promotion system constructed with the seven components of the present invention can increase the stability of macromolecular efficacy components such as astaxanthin, increase the contact time of astaxanthin with the skin, and thereby increase the utilization rate of astaxanthin.

Test Example 3 Promotional Effect of Anti-Aging Ingredients 1. The wrinkles around the eyes and face were photographed with a Visia camera, and data on skin age was recorded.
2. By adding 1% of an anti-wrinkle component (anti-wrinkle complex peptide) to Example 1, Comparative Example 1, and Comparative Example 8, compositions having anti-aging effects corresponding to Application Examples 22, 23, and 24, respectively, were obtained.
3. Application Examples 22, 23, and 24 were applied twice a day for 12 weeks to each area, and photos were taken with a Visia camera every two weeks to record skin age data.
4. The number of subjects was 10 for each group, and the skin age was the average value.

Conclusion: The efficacy-promoting composition prepared in the embodiment of the present invention can effectively promote the efficacy of the anti-aging ingredient. As can be seen from the above table, in application example 22, the efficacy of the anti-aging ingredient increased significantly, and after a certain period of use, the skin age decreased by 6 years, while in application examples 23 and 24, after a certain period of use, the change in skin age was relatively small or almost unchanged.

Claims (12)

An efficacy enhancing composition comprising polymeric hyaluronic acid or a salt thereof, hydrolyzed hyaluronic acid or a salt thereof, acetylated hyaluronic acid or a salt thereof, tetrahydropyrimidine or a derivative thereof, ergothioneine, Saccharomyces/Rice Ferment Filtrate , and aminobutyric acid ;
the polymeric hyaluronic acid or a salt thereof is 1 to 30% by mass, the hydrolyzed hyaluronic acid or a salt thereof is 2 to 50% by mass, the acetylated hyaluronic acid or a salt thereof is 1 to 10% by mass, the tetrahydropyrimidine or a derivative thereof is 0.1 to 10% by mass, the ergothioneine is 2 to 20% by mass, the Saccharomyces/rice fermentation broth is 10 to 50% by mass, and the aminobutyric acid is 0.1 to 5% by mass;
The molecular weight of the high molecular weight hyaluronic acid or its salt is 1000 to 1800 kDa; and
The molecular weight of the hydrolyzed hyaluronic acid or its salt is 1 to 10 kDa;
Composition. The composition according to claim 1, characterized in that the polymeric hyaluronic acid or its salt is 1 to 10% by mass, the hydrolyzed hyaluronic acid or its salt is 2 to 10% by mass, the acetylated hyaluronic acid or its salt is 1 to 1.5% by mass, the tetrahydropyrimidine or its derivative is 0.3 to 5% by mass, the ergothioneine is 3 to 10% by mass, the Saccharomyces/rice fermentation broth is 15 to 40% by mass, and the aminobutyric acid is 0.3 to 3% by mass. The composition according to claim 1 or 2, characterized in that, on a weight part basis, the polymeric hyaluronic acid or its salt is 1 part by weight, the hydrolyzed hyaluronic acid or its salt is 0.2 to 2 parts by weight, and the acetylated hyaluronic acid or its salt is 0.05 to 1.5 parts by weight. The composition according to claim 3, wherein, on a weight part basis, the polymeric hyaluronic acid or its salt is 1 part by weight, the hydrolyzed hyaluronic acid or its salt is 0.5 to 1 part by weight, and the acetylated hyaluronic acid or its salt is 0.1 to 0.5 parts by weight. The composition according to any one of claims 1 to 4 , characterized in that the molecular weight of the high molecular weight hyaluronic acid or its salt is 1,000 to 1,500 kDa. The composition according to any one of claims 1 to 5 , wherein the molecular weight of the hydrolyzed hyaluronic acid or its salt is between 5 kDa and 10 kDa. The composition according to any one of claims 1 to 6 , characterized in that the molecular weight of the acetylated hyaluronic acid or its salt is 20 kDa to 100 kDa and the acetyl group is 20 to 40 mass %. The composition according to any one of claims 1 to 7 , wherein the tetrahydropyrimidine or a derivative thereof is one or two selected from ectoine and hydroxyectoine . 9. The composition according to claim 8, wherein the tetrahydropyrimidine or derivative thereof is ectoine. The composition according to any one of claims 1 to 9, further comprising a preservative, the preservative being 0.1 to 5 mass% in the composition and being one or more preservatives selected from pentylene glycol, hexylene glycol, butylene glycol and ethylhexylglycerin. Use of the composition according to any one of claims 1 to 10 in skin care products or cosmetics. A skin care product or cosmetic comprising the composition according to any one of claims 1 to 10 .