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JP7605584B2 - Method for producing baked goods - Google Patents
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JP7605584B2 - Method for producing baked goods - Google Patents

Method for producing baked goods Download PDF

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JP7605584B2
JP7605584B2 JP2019190068A JP2019190068A JP7605584B2 JP 7605584 B2 JP7605584 B2 JP 7605584B2 JP 2019190068 A JP2019190068 A JP 2019190068A JP 2019190068 A JP2019190068 A JP 2019190068A JP 7605584 B2 JP7605584 B2 JP 7605584B2
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starter
liquid fermentation
lactic acid
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yeast
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JP2021061808A (en
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裕一 池田
美香子 中村
謙 貝沼
雅行 小倉
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Yamazaki Baking Co ltd
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Yamazaki Seipan KK
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Description

本発明は、パイ菓子などの焼き菓子の製造方法に関する。特に本発明は、液状醗酵種を用いる焼き菓子の製造方法に関する。 The present invention relates to a method for producing baked goods such as pie pastries. In particular, the present invention relates to a method for producing baked goods using a liquid fermentation starter.

一般にパン類の製造に関しては、従来から、製品の風味改善を目的として、乳酸菌等を用いて醗酵種を調製し、調製した醗酵種をパン類生地に添加して醗酵させて焼成することによるパンの製造方法が知られている。例えば、特許文献1~2には、パン類の製造に関して、乳酸菌を用いて醗酵種を調製する方法が開示されている。 In general, in the production of bread, a method has been known in which a leavening agent is prepared using lactic acid bacteria or the like, and the prepared leavening agent is added to bread dough, which is then fermented and baked in order to improve the flavor of the product. For example, Patent Documents 1 and 2 disclose methods for preparing a leavening agent using lactic acid bacteria in the production of bread.

特開平11-266775号公報Japanese Patent Application Publication No. 11-266775 特開2003-79307号公報JP 2003-79307 A

パン類とは異なり、パイ菓子などの焼き菓子の製造において、醗酵工程を採用することは一般的ではない。醗酵工程を採用しないことから、焼き菓子は、醗酵によって得られる深みのある風味(醗酵風味)に欠け、食感が硬くなる傾向がある。 Unlike breads, it is not common to use a fermentation process when making baked goods such as pies. Because no fermentation process is used, baked goods tend to lack the deep flavor (fermented flavor) that can be obtained through fermentation and have a hard texture.

醗酵工程を採らないで製造する焼き菓子に欠けている醗酵風味などを補うために、単に醗酵種を生地に添加して焼成した焼き菓子は、刺激的な酸臭・酸味が強く、マイルドで爽やかなサワー風味や香ばしさに欠けていた。また、味覚的にも、旨味・甘味(あまみ)・コク(味わい)に欠けるところがあった。 In order to compensate for the lack of fermented flavor in baked goods made without a fermentation process, baked goods that are simply made by adding fermentation starter to the dough have a strong, pungent sour smell and taste, and lack the mild, refreshing sour flavor and aroma. In terms of taste, they also lack umami, sweetness, and richness.

そこで、本発明の課題は、醗酵種を用いてパイ菓子等の焼き菓子を製造するにあたり、刺激的な強い酸味・酸臭を抑制しつつ、優れた香味を有する焼き菓子を製造することである。 The object of the present invention is to produce baked goods such as pie pastries using a fermentation starter, while suppressing the strong, irritating sour taste and smell, and producing baked goods with excellent flavor.

本発明者らは、従来の技術に関する上記課題を解決すべく鋭意研究を行った結果、乳酸菌および酵母菌を使用して調製した醗酵種を添加することにより、極めて好ましい香ばしさを有する焼き菓子を製造できることを見出し、本発明を完成した。 As a result of intensive research conducted by the inventors to solve the above problems associated with conventional technology, they discovered that by adding a fermentation agent prepared using lactic acid bacteria and yeast, baked goods with an extremely desirable fragrant flavor could be produced, and thus completed the present invention.

すなわち、これに限定されるものではないが、本発明は以下の事項に関する。
[1] 小麦粉、水、乳酸菌および酵母菌を少なくとも含む原料を醗酵させて液状醗酵種を調製する工程と、生地に使用する穀粉の全量に対して、0.5~12質量%の液状醗酵種を添加して生地を作成する工程と、生地を焼成して焼き菓子を得る工程と、を含む、焼き菓子の製造方法。
[2] 前記液状醗酵種に含まれる活性乳酸菌の菌数が、前記液状醗酵種に含まれる活性酵母菌の菌数よりも多い、[1]に記載の方法。
[3] 前記液状醗酵種に含まれる活性乳酸菌の菌数が、前記液状醗酵種に含まれる活性酵母菌の菌数よりも10~10/g以上多い、[1]または[2]に記載の方法。
[4] 前記液状醗酵種に含まれる活性乳酸菌の菌数が10~1010/gであり、前記液状醗酵種に含まれる活性酵母菌の菌数が10~10/gである、[1]~[3]のいずれかに記載の方法。
[5] 前記液状醗酵種のpHが4以下である、[1]~[4]のいずれかに記載の方法。
[6] 前記液状醗酵種の有機酸含有量が4000ppm以上である、[1]~[5]のいずれかに記載の方法。
[7] 前記液状醗酵種の乳酸含有量が3500ppm以上である、[1]~[6]のいずれかに記載の方法。
[8] 前記生地をイースト醗酵させないで焼成する、[1]~[7]のいずれかに記載の方法。
[9] 前記焼き菓子がパイ菓子である、[1]~[8]のいずれかに記載の方法。
That is, although not limited thereto, the present invention relates to the following:
[1] A method for producing baked goods, comprising the steps of: fermenting a raw material containing at least wheat flour, water, lactic acid bacteria, and yeast to prepare a liquid fermented starter; preparing dough by adding 0.5 to 12 mass% of the liquid fermented starter relative to the total amount of grain flour used in the dough; and baking the dough to obtain a baked goods.
[2] The method according to [1], wherein the number of active lactic acid bacteria contained in the liquid fermentation species is greater than the number of active yeast bacteria contained in the liquid fermentation species.
[3] The method according to [1] or [2], wherein the number of active lactic acid bacteria contained in the liquid fermentation agent is 10 8 to 10 9 /g or more higher than the number of active yeast bacteria contained in the liquid fermentation agent.
[4] The method according to any one of [1] to [3], wherein the number of active lactic acid bacteria contained in the liquid fermentation agent is 10 to 10 /g, and the number of active yeast bacteria contained in the liquid fermentation agent is 10 to 10 /g.
[5] The method according to any one of [1] to [4], wherein the pH of the liquid fermentation seed is 4 or less.
[6] The method according to any one of [1] to [5], wherein the organic acid content of the liquid fermentation species is 4000 ppm or more.
[7] The method according to any one of [1] to [6], wherein the liquid fermentation species has a lactic acid content of 3,500 ppm or more.
[8] The method according to any one of [1] to [7], wherein the dough is baked without yeast fermentation.
[9] The method according to any one of [1] to [8], wherein the baked confectionery is a pie confectionery.

本発明に基づいて、活性乳酸菌および活性酵母菌を含有する液状醗酵種を生地に添加して焼き菓子を焼成することにより、刺激的な強い酸味・酸臭を抑制しつつ、優れた香味を有する焼き菓子を製造することが可能になる。 Based on the present invention, by adding a liquid fermentation starter containing active lactic acid bacteria and active yeast to the dough and baking the baked goods, it is possible to produce baked goods with excellent flavor while suppressing the strong, irritating sour taste and smell.

図1は、実験2で焼成したパイ菓子の外観写真である(左から順に、サンプル1からサンプル7である)。FIG. 1 is a photograph showing the appearance of the pie pastries baked in Experiment 2 (from left to right, Sample 1 to Sample 7).

本発明は、少なくとも小麦粉、水、乳酸菌および酵母菌を含む原料を醗酵させて調製する液状醗酵種を使用する。本発明においては、活性乳酸菌および活性酵母菌を含有する液状醗酵種を、焼き菓子の生地に添加するが、生地の作成に使用する穀粉の全量に対し、0.5~10質量%の液状醗酵種を添加して生地を作成する。 The present invention uses a liquid fermentation starter prepared by fermenting ingredients containing at least wheat flour, water, lactic acid bacteria, and yeast. In the present invention, a liquid fermentation starter containing active lactic acid bacteria and active yeast is added to the dough of baked goods, and the dough is prepared by adding 0.5 to 10% by mass of the liquid fermentation starter to the total amount of grain flour used to prepare the dough.

本発明において、液状醗酵種とは、流動性があり、かつ保形性および可塑性に欠ける性状の醗酵種を意味し、少なくとも小麦粉、水、活性乳酸菌および活性酵母菌から構成されるものである。 In the present invention, a liquid fermentation starter means a fermentation starter that is fluid and lacks shape retention and plasticity, and is composed of at least wheat flour, water, active lactic acid bacteria, and active yeast.

本発明に係る液状醗酵種を構成する小麦粉としては、例えば、強力粉、中力粉、薄力粉、荒挽粉、全粒粉、デュラム粉などを使用することができ、複数種の小麦粉を併用することもできる。また、小麦粉以外の穀粉として、例えば、ライ麦粉、大麦粉などの穀粉が挙げられ、これらを小麦粉とともに併用することもできる。 As the wheat flour constituting the liquid fermentation starter according to the present invention, for example, strong flour, medium flour, weak flour, coarsely ground flour, whole wheat flour, durum flour, etc. can be used, and multiple types of wheat flour can also be used in combination. In addition, examples of grain flour other than wheat flour include rye flour, barley flour, etc., and these can also be used in combination with wheat flour.

本発明に係る液状醗酵種を製造する場合、小麦粉を含む穀粉と水の構成割合は特に制限されず、従来使用されてきた割合を用いることができる。具体的には、例えば、小麦粉を含む穀粉に対して50~200質量%の水を使用することが好ましく、100~150質量%の水を使用することがより好ましい。 When producing the liquid fermentation starter of the present invention, the composition ratio of the flour, including wheat flour, and water is not particularly limited, and the ratios that have been used conventionally can be used. Specifically, for example, it is preferable to use 50 to 200% by mass of water relative to the flour, including wheat flour, and it is more preferable to use 100 to 150% by mass of water.

本発明において、液状醗酵種を構成する乳酸菌および酵母菌を添加する形態は特に制限されず、人為的に培養した菌体を添加してもよいし、穀粉などに付着している自然菌を液状醗酵種中に取り込ませてもよい。スターターとして乳酸菌や酵母菌を人為的に添加する場合、例えば、菌体、醗酵液、醗酵物、これらの乾燥粉末、元種などの1種または2種以上を添加することができる。単離培養菌体は、例えば、集菌した菌を寒天培地などで培養して得ることができ、乳酸菌および酵母菌からなるものとしたり、乳酸菌および酵母菌を含むものとしたりすることができる。自然菌を利用する場合、例えば、小麦粉やライ麦粉等の小麦粉以外の穀粉を添加することに伴い、これらの穀粉に付着している自然菌を液状醗酵種中に取り込むことができる。本発明においては、人為的添加の形態と自然菌利用の形態とを併用してもよい。 In the present invention, the form in which the lactic acid bacteria and yeast that constitute the liquid fermentation starter are added is not particularly limited, and artificially cultured bacterial cells may be added, or natural bacteria attached to grain flour or the like may be incorporated into the liquid fermentation starter. When lactic acid bacteria or yeast are artificially added as a starter, for example, one or more of bacterial cells, fermentation liquid, fermented product, dried powders of these, and starting materials may be added. Isolated cultured bacterial cells can be obtained, for example, by culturing collected bacteria on an agar medium or the like, and can be made of lactic acid bacteria and yeast or contain lactic acid bacteria and yeast. When natural bacteria are used, for example, by adding grain flour other than wheat flour, such as wheat flour or rye flour, natural bacteria attached to these grain flours can be incorporated into the liquid fermentation starter. In the present invention, the form of artificial addition and the form of using natural bacteria may be used in combination.

ここで、本明細書において「種起こし」とは、最初の液状醗酵種(当業界において、初種、親種、一番種などと呼ばれることがあり、本明細書においては初種ともいう)の調製を意味する。さらに、本明細書において「種継ぎ」とは、種起こしした初種を用いた醗酵種の調製と、種継ぎした醗酵種を用いた1回又は複数回の醗酵種の調製とのそれぞれを含む意味である。具体的には、例えば、種継ぎを行わないで、醗酵種を調製する場合、初めの種起こしのみを行って醗酵種を調製することになる。また、種起こしおよび種継ぎを合計5回行って醗酵種を調製する場合、初めの種起こしを行った後に、種継ぎを4回行うことになる。さらに、種起こしおよび種継ぎを合計10回行って醗酵種を調製する場合、初めの種起こしを行った後に、種継ぎを9回行うことになる。 Here, in this specification, "starting" refers to the preparation of the first liquid fermentation starter (sometimes called the first starter, parent starter, or first starter in the industry, and also referred to as the first starter in this specification). Furthermore, in this specification, "passing" refers to both the preparation of a fermentation starter using a started first starter and the preparation of a fermentation starter once or multiple times using a passed fermentation starter. Specifically, for example, when preparing a fermentation starter without passing, the fermentation starter is prepared by only the first starter. Furthermore, when preparing a fermentation starter by performing starter raising and passing five times in total, the first starter is followed by four passes. Furthermore, when preparing a fermentation starter by performing starter raising and passing ten times in total, the first starter is followed by nine passes.

より具体的には、最初の液状醗酵種を調製する場合(すなわち、種起こしする場合)、乳酸菌および酵母菌を添加する形態としては、乳酸菌および酵母菌からなる、またはこれらを含む単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他のうちの1種または2種以上を添加してもよく、また、このような乳酸菌および酵母菌そのものを添加するとともに、または添加することなく、醗酵種を構成する小麦粉や小麦粉以外の穀粉に付着している自然菌を利用することもできる。 More specifically, when preparing the initial liquid fermentation starter (i.e., when starting), the lactic acid bacteria and yeast may be added in the form of one or more of isolated cultured cells consisting of or containing lactic acid bacteria and yeast, a fermentation liquid, a fermented product, dried powders of these, and others. It is also possible to use natural bacteria attached to the wheat flour or grain flour other than wheat flour that constitutes the fermentation starter, with or without adding such lactic acid bacteria and yeast themselves.

また、初種を基礎として(元種として)1回、または初種から出発して(初種の少なくとも一部を最初の元種として)複数回、もしくは繰り返し無数に種継ぎを行う場合、それぞれの種継ぎにおいて、乳酸菌および酵母菌を添加する形態として、元種に含有される乳酸菌および酵母菌や、種継ぎの際に添加する醗酵種を構成する小麦粉や小麦粉以外の穀粉に付着している自然菌を利用することができ、さらに必要に応じて、前記単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他のうちの1種または2種以上を添加することもできる。 In addition, when the seed is passed once based on the initial seed (as the original seed), or multiple times starting from the initial seed (using at least a part of the initial seed as the initial original seed), or repeatedly and indefinitely, the lactic acid bacteria and yeast bacteria added in each pass can be those contained in the original seed, or natural bacteria attached to wheat flour or grain flour other than wheat flour that constitutes the fermented seed added during the pass, and further, if necessary, one or more of the isolated cultured bacteria, fermentation liquid, fermented product, dried powders of these, and others can be added.

種起こしにおいても、種継ぎにおいても、前記単離培養菌体、醗酵液、醗酵物、これらの乾燥粉末、その他の乳酸菌および酵母菌そのものを添加しない場合には、通常、菌の増殖を図るため、比較的長時間の醗酵が必要とされる。 In both starter and propagation, if the isolated cultured cells, fermentation liquid, fermented product, or dried powders of these, or other lactic acid bacteria or yeasts themselves are not added, a relatively long fermentation time is usually required to promote bacterial growth.

例えば、単離培養菌体などの乳酸菌および酵母菌そのものを添加しなければ、必要時間醗酵させたとしても、必要な、または十分な菌の増殖を図ることができないことがある。この場合、単離培養菌体などの乳酸菌および酵母菌そのものを添加することにより、増殖菌数の不足分を補足することができる。この場合、通常、著しく長時間の醗酵を必要としない。 For example, if lactic acid bacteria such as isolated cultured cells and yeast cells themselves are not added, the necessary or sufficient bacterial growth may not be achieved even if fermentation is carried out for the required time. In this case, the shortage in the number of grown bacteria can be supplemented by adding lactic acid bacteria such as isolated cultured cells and yeast cells themselves. In this case, fermentation for an extremely long period of time is usually not required.

本発明において、種起こしおよび/または種継ぎで前記単離培養菌体その他の乳酸菌および酵母菌そのものを添加するとしても、液状醗酵種中の菌数、醗酵条件、菌の増殖の早さ、求める最終醗酵状態等の事情に応じて、種起こし時だけに添加してもよいし(種継ぎには添加しない)、また、種起こし時には添加せずに、種継ぎだけに添加するようにしてもよい。さらに本発明においては、種起こしおよび種継ぎの両者で添加するようにしてもよい。さらにまた、種継ぎで添加する際も、それぞれの種継ぎごとに毎回添加するようにしてもよいし、種継ぎの1回おき、2回おき、3回おき、というように間隔をあけて定期的に添加してもよく、もしくは、任意に不規則的に時々添加してもよい。 In the present invention, even if the isolated cultured bacteria and other lactic acid bacteria and yeasts themselves are added in the starter and/or the succession, they may be added only in the starter (not in the succession), or they may be added only in the succession without being added in the starter, depending on the number of bacteria in the liquid fermentation starter, the fermentation conditions, the rate of bacterial growth, the desired final fermentation state, etc. Furthermore, in the present invention, they may be added in both the starter and the succession. Furthermore, when they are added in the succession, they may be added every time after each succession, or they may be added periodically at intervals such as every other, every second, or every third succession, or they may be added occasionally at any irregular interval.

同じく乳酸菌の菌種としては、本発明の目的・効果を妨げるものでなければ、特に制限はなく、いずれのものも使用できる。好適には、グルコース等の糖類から多量の乳酸を生成する菌で、ラクトバチルス属、ロイコノストック属、ストレプトコッカス属、ペディオコッカス属等に属するものである。具体的には、例えば、ラクトバチルス・プランタラム、ラクトバチルス・アシドフィラス、ラクトバチルス・ヘルベティカス、ラクトバチルス・ブレビス、ラクトバチルス・ブルガリカス、ラクトバチルス・サンフランシスコ、ラクトバチルス・イタリカス、ラクトバチルス・ヒルガルディ、ラクトバチルス・カゼイ、ラクトバチルス・ファーメンタム、ラクトバチルス・デルブルッキー、ラクトバチルス・ライヒマニ、ラクトバチルス・パストリアヌス、ラクトバチルス・ブヒネリ、ラクトバチルス・フルクティボランス、ラクトバチルス・ルテリ、ラクトバチルス・パラアリメンタリウス、ロイコノストック・オイアノス、ロイコノストック・デキストラニクム、ロイコノストック・クレモリス、ストレプトコッカス・サーモフィラス、ストレプトコッカス・クレモリス、ストレプトコッカス・ラクティス、ストレプトコッカス・ジアセチラクチス、ストレプトコッカス・サリバリウス、ストレプトコッカス・フェカリス、ペディオコッカス・ペントサシウス、ペディオコッカス・ハロフイラス、ペディオコッカス・アシディラクティシ、ペディオコッカス・サセウス、ペディオコッカス・セレビジェ、ペディオコッカス・ダノムサス、ペディオコッカス・デキストリニカス、ペディオコッカス・ホマリ、ペディオコッカス・イノピナタス、ペディオコッカス・パルバラス、エンテロコッカス・フェカリス、テトラゲノコッカス・ハロフィラス、ラクトコッカス・ラクティス等が挙げられる。これらの乳酸菌を単独で、または複数種を組み合わせて使用することができる。 Similarly, there are no particular limitations on the species of lactic acid bacteria, and any can be used as long as it does not interfere with the objectives and effects of the present invention. Preferably, the species is a bacterium that produces a large amount of lactic acid from sugars such as glucose, and belongs to the genera Lactobacillus, Leuconostoc, Streptococcus, Pediococcus, etc. Specific examples of the lactobacillus include Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus san francisco, Lactobacillus italicus, Lactobacillus hilgardii, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus delbrueckii, Lactobacillus reichmanii, Lactobacillus pasteurianus, Lactobacillus buhineri, Lactobacillus fructivorans, Lactobacillus luteri, Lactobacillus paraalimentarius, Leuconostoc oianos, Leuconostoc dextranicum, and Leuconostoc cremoris. , Streptococcus thermophilus, Streptococcus cremoris, Streptococcus lactis, Streptococcus diacetylactis, Streptococcus salivarius, Streptococcus faecalis, Pediococcus pentosacius, Pediococcus halophilus, Pediococcus acidilactici, Pediococcus saceus, Pediococcus cerevisiae, Pediococcus danomus, Pediococcus dextrinicus, Pediococcus homari, Pediococcus inopinatus, Pediococcus parvalas, Enterococcus faecalis, Tetragenococcus halophilus, Lactococcus lactis, etc. These lactic acid bacteria can be used alone or in combination of multiple types.

同じく酵母菌の菌種としては、本発明の目的・効果を妨げるものでなければ、特に制限はなく、いずれのものも使用できる。具体的には、例えば、サッカロミセス・セレビシエ、サッカロミセス・ロゼイ、サッカロミセス・クルバータス、サッカロミセス・シュバリエリ、サッカロミセス・フロレンティナス、サッカロミセス・エグジグアス、サッカロミセス・バイリイ、クルイベロマイセス・ラクティス、トルラスポラ・デルブルッキー、キャンディダ・ユティリス、キャンディダ・ケフィア等があげられる。これらのイーストを単独で、または複数種を組み合わせて使用することができる。 Similarly, there are no particular limitations on the species of yeast fungus, and any species can be used as long as it does not interfere with the objectives and effects of the present invention. Specific examples include Saccharomyces cerevisiae, Saccharomyces rosei, Saccharomyces curvertus, Saccharomyces chevalieri, Saccharomyces florentinus, Saccharomyces exiguas, Saccharomyces baillii, Kluyveromyces lactis, Torulaspora delbrueckii, Candida utilis, Candida kefir, and the like. These yeasts can be used alone or in combination of multiple species.

本発明では、これらの酵母菌と上述の乳酸菌を併用する。本発明に係る液状醗酵種を調製するにあたり、醗酵・培養開始前における当初の該液状醗酵種中の乳酸菌および酵母菌のそれぞれは、例えば、液状醗酵種1グラム中に10~10CFU(colony forming unit)の菌が存在するように添加することができる。 In the present invention, these yeasts are used in combination with the above-mentioned lactic acid bacteria. When preparing the liquid fermentation starter according to the present invention, the lactic acid bacteria and yeasts in the initial liquid fermentation starter before the start of fermentation and culture can be added so that, for example, 10 to 10 CFU (colony forming unit) of bacteria are present in 1 gram of the liquid fermentation starter.

本発明に係る液状醗酵種には糖類を含むことが望ましく、糖類としては、添加する乳酸菌および酵母菌が資化できる糖であればよく、例えば、ブドウ糖、果糖、ショ糖、麦芽糖、乳糖、はちみつ、その他のものを用いることができる。そして、これらの糖類を1種または複数種組み合わせて用いることもできる。糖類の含有量は、該液状醗酵種を構成する小麦粉などの穀粉に対して1~10質量%が望ましい。 The liquid fermentation starter according to the present invention preferably contains sugars, and the sugars may be any sugar that can be assimilated by the added lactic acid bacteria and yeast, such as glucose, fructose, sucrose, maltose, lactose, honey, and others. These sugars may be used alone or in combination. The sugar content is preferably 1 to 10% by mass of the wheat flour or other grain flour that constitutes the liquid fermentation starter.

本発明に係る液状醗酵種を構成するその他の原料としては、従来醗酵種に使用されてきたものを用いることができる。具体的には、例えば、乳製品、油脂、塩、モルト、増粘剤等、本発明の目的・効果を妨げないものであれば、いずれのものも用いることができる。 Other raw materials constituting the liquid fermentation starter of the present invention can be those that have been used in conventional fermentation starters. Specifically, for example, dairy products, oils and fats, salt, malt, thickeners, etc., can be used as long as they do not interfere with the purpose and effect of the present invention.

本発明では、液状醗酵種として、種起しにより最初に調製して醗酵させた醗酵種(初種)を使用することができるし、または該初種から出発し、この初種の少なくとも一部を最初の元種にして種継ぎを1回または複数回もしくは無数に行って作成する醗酵種(以下継ぎ種という)を使用することができる。 In the present invention, the liquid fermentation starter can be a fermentation starter (initial starter) that is initially prepared and fermented by starting with a seed, or a fermentation starter (hereinafter referred to as a succession starter) can be prepared by starting from the initial starter and using at least a portion of this initial starter as the initial starting starter and succession once, multiple times, or an infinite number of times.

本発明に係る液状醗酵種は、初種および種継ぎともに、従来の方法によって調製することができる。具体的には、例えば、初種は原料を10~30分間攪拌した後、27~33℃で15~24時間培養することができる。そして、培養中には、定期的に間欠攪拌することが好ましい。以下の種継ぎをしないときには、攪拌後の元種を長時間、好ましくは17~21時間培養したものを使用することが望ましい。 The liquid fermentation starter according to the present invention, both the initial starter and the successive starter, can be prepared by conventional methods. Specifically, for example, the initial starter can be prepared by stirring the raw materials for 10 to 30 minutes, and then culturing at 27 to 33°C for 15 to 24 hours. It is preferable to carry out periodic intermittent stirring during culturing. When the following successive starter is not carried out, it is desirable to use the initial starter after stirring that has been cultured for a long period of time, preferably 17 to 21 hours.

種継ぎの方法としても、従来の方法を用いることができる。具体的には、例えば、前記液状醗酵種と同様の原料及び使用割合、即ち、小麦粉100質量%、水50~150質量%、前記元種10~50質量%、必要に応じて乳酸菌および酵母菌(適宜量)、糖類1~10質量%とを混合・攪拌し、27~33℃で6~12時間培養するようにする。 Conventional methods can also be used for the seed propagation method. Specifically, for example, the same raw materials and usage ratios as those for the liquid fermentation starter are mixed and stirred as follows: 100% by weight of wheat flour, 50-150% by weight of water, 10-50% by weight of the starter, and, if necessary, lactic acid bacteria and yeast (appropriate amounts), and 1-10% by weight of sugar, and then cultured at 27-33°C for 6-12 hours.

本発明の液状醗酵種は、初種でも、または初種から出発して種継ぎをした継ぎ種でも、醗酵種として直ちにパイ菓子の製造に使用することができる程度まで培養して醗酵させておくことが望ましい。このためには、焼き菓子生地に該液状醗酵種を添加する工程において、該液状醗酵種の活性乳酸菌および活性酵母菌の菌数が、それぞれ10~1010/g、10~10/gであるように調整しておくことが望ましい。また、該液状醗酵種は、活性乳酸菌の菌数が活性酵母菌の菌数よりも多く含有していることが望ましく、具体的には、例えば、該液状醗酵種が、含有する活性乳酸菌の菌数が活性酵母菌の菌数よりも10~10/g以上多くなるように調整しておくことが望ましい。これにより、酵母臭や酵母風味のない、マイルドで爽やかなサワー風味と、香ばしい香と、旨味・甘味・コク味が一層豊かな該焼き菓子を製造することが可能になる。 The liquid fermentation starter of the present invention, whether it is a primary starter or a succession starter which has been started from the primary starter and then successively propagated, is preferably cultured and fermented to an extent that it can be used immediately as a fermentation starter in the manufacture of pie pastries. For this purpose, in the step of adding the liquid fermentation starter to the baked confectionery dough, it is preferable to adjust the cell counts of the active lactic acid bacteria and active yeast bacteria in the liquid fermentation starter to 106-1010 / g and 105-109 /g , respectively. It is also preferable that the liquid fermentation starter contains more active lactic acid bacteria than active yeast bacteria, and specifically, for example, it is preferable to adjust the liquid fermentation starter so that the cell count of the active lactic acid bacteria is 108-109 /g or more more than the cell count of the active yeast bacteria . This makes it possible to produce baked goods that are free from yeast odor or yeast flavor, have a mild and refreshing sour flavor, a fragrant aroma, and are richer in umami, sweetness, and richness.

また、初種および継ぎ種ともに、パイ菓子生地の混捏工程で添加する液状醗酵種のpHを4.0以下となるまで、具体的には、例えば、3.0~4.0となるまで醗酵させておくことが望ましく、3.2~3.8となるまで醗酵させることがより望ましく、3.3~3.7となるまで醗酵させることがより一層望ましい。これにより、醗酵終了後の液状醗酵種中の活性乳酸菌および活性酵母菌の数量が著しく増大することにより、該液状醗酵種を含有する焼き菓子生地を使用して製造した焼成後の焼き菓子は、より一層良好な豊かな風味を有する。 In addition, for both the initial starter and the second starter, it is desirable to ferment the liquid fermented starter added during the kneading process of the pie pastry dough until the pH becomes 4.0 or less, specifically, for example, until it becomes 3.0 to 4.0, more preferably until it becomes 3.2 to 3.8, and even more preferably until it becomes 3.3 to 3.7. This significantly increases the number of active lactic acid bacteria and active yeast bacteria in the liquid fermented starter after fermentation is complete, and baked goods produced using the baked goods dough containing the liquid fermented starter have an even better, richer flavor after baking.

そして、本発明では、焼き菓子生地に該液状醗酵種を添加する工程において、該液状醗酵種の有機酸含有量が4000ppm以上であることが望ましい。これにより、生地の伸展性と風味の向上が図られる。特に、該液状醗酵種の乳酸含有量が3500ppm以上であることが望ましく、4000ppm以上であることがより望ましい。これにより、生地の伸展性と風味の大幅な向上が図られる。 In the present invention, in the step of adding the liquid fermentation starter to the baked confectionery dough, it is desirable that the organic acid content of the liquid fermentation starter is 4000 ppm or more. This improves the extensibility and flavor of the dough. In particular, it is desirable that the lactic acid content of the liquid fermentation starter is 3500 ppm or more, and more desirably 4000 ppm or more. This greatly improves the extensibility and flavor of the dough.

本発明の焼き菓子としてはパイ菓子が最も好適である。パイ菓子には、小麦粉などの穀粉を主体とする部分と油脂を主体とする部分があり、例えば、小麦粉及び食用油脂を原料とし、必要により澱粉、糖類、乳製品、卵製品、イースト、膨脹剤、食品添加物等の原材料を配合し、又は添加したものを混合機、成型機及びビスケットオーブンを使用して製造することができる。本発明は、小麦粉を主体とする部分と油脂とが交互に層状になった折パイに適用することもでき、油脂がまんべんなく分散した練パイに適用することもできる。 Pie confectionery is most suitable as the baked confectionery of the present invention. Pie confectionery has a part mainly made of flour such as wheat flour and a part mainly made of fats and oils. For example, it can be made using wheat flour and edible fats and oils as ingredients, with starch, sugars, dairy products, egg products, yeast, leavening agents, food additives and other ingredients mixed or added as necessary, using a mixer, molding machine and biscuit oven. The present invention can be applied to folded pies in which the flour-based part and fats and oils are layered alternately, and also to kneaded pies in which fats and oils are evenly distributed.

本発明では、前記焼き菓子の生地をイースト醗酵させないようにすることが望ましい。 In the present invention, it is desirable to avoid yeast fermentation of the dough for the baked confectionery.

以下、本発明の実施例を説明しながら本発明について詳述するが、本発明は、以下の実施例に限定されるものではない。なお、本明細書において、特に記載しない限り、数値範囲はその端点を含むものして記載されて、濃度などは重量基準である。 The present invention will be described in detail below with reference to examples of the present invention, but the present invention is not limited to the following examples. In this specification, unless otherwise specified, numerical ranges are stated including their endpoints, and concentrations, etc. are based on weight.

実験1.液状醗酵種の調製と評価
1-1.液状醗酵種の調製
小麦粉(強力粉)100質量%および水150質量%の混合物に、下表に示す元種を、小麦粉に対し0.5質量%添加し、30℃にて18時間間歇攪拌下で培養させて液状醗酵種を調製し、培養後の液状醗酵種を温度10℃以下で最長24時間まで静置条件下下で保存した。このようにして調製された醗酵種は、とろみがあり、乳酸醗酵特有の香りを有していた(pH:約3.5)。
Experiment 1. Preparation and evaluation of liquid fermentation starter 1-1. Preparation of liquid fermentation starter The starter shown in the table below was added at 0.5% by mass to a mixture of 100% by mass wheat flour (strong flour) and 150% by mass water, and the mixture was cultured at 30°C for 18 hours with intermittent stirring to prepare a liquid fermentation starter. The cultured liquid fermentation starter was stored under static conditions at a temperature of 10°C or less for up to 24 hours. The fermentation starter prepared in this way was viscous and had a unique aroma of lactic acid fermentation (pH: about 3.5).

本実験で用いた元種は、凍結乾燥された粉末であり、酵母としてサッカロミセス・シュバリエリを99質量%(酵母菌1.10cfu/g)、乳酸菌としてラクトバチラス・カゼイ(ホモ型)およびラクトバチラス ・ブレビス(ヘテロ型)をそれぞれ0.5質量%ずつ含有する。このような凍結乾燥粉末は、例えば、ルサッフル社(フランス、商品名:サフルヴァン)などから入手可能である。 The original seed used in this experiment is a freeze-dried powder, containing 99% by mass of Saccharomyces chevalieri as yeast (yeast bacteria 1.10 9 cfu/g), and 0.5% by mass each of Lactobacillus casei (homotype) and Lactobacillus brevis (heterotype) as lactic acid bacteria. Such freeze-dried powder is available, for example, from Lesaffre (France, product name: Safulvan).

Figure 0007605584000001
Figure 0007605584000001

1-2.液状醗酵種の評価
上記のようにして得られた液状醗酵種について、醗酵種に含まれる有機酸の分析を行った。結果を下表に示すが、液状醗酵種中における有機酸の合計含有量は4319ppmであった。特に、焼成した焼き菓子の香り、風味等の官能特性に寄与する乳酸の含有量は4010ppmであり、全体的な有機酸の合計含有量の約93質量%を占めるほど高含有量であった。これに対し、焼き菓子の香り、風味等の官能特性に悪影響を与えるクエン酸、リンゴ酸、コハク酸及び酢酸の液状醗酵種中における合計含有量は、僅かに309ppmであり、全体的な有機酸の合計含有量の約7.2質量%程度であった。
1-2. Evaluation of liquid fermented starter The organic acids contained in the liquid fermented starter obtained as described above were analyzed. The results are shown in the table below. The total content of organic acids in the liquid fermented starter was 4319 ppm. In particular, the content of lactic acid, which contributes to the sensory properties of baked goods such as the aroma and flavor, was 4010 ppm, which was a high content of about 93% by mass of the total organic acid content. In contrast, the total content of citric acid, malic acid, succinic acid, and acetic acid, which have a negative effect on the sensory properties of baked goods such as the aroma and flavor, in the liquid fermented starter was only 309 ppm, which was about 7.2% by mass of the total organic acid content.

有機酸、特に乳酸を多く含むことにより、本発明の醗酵種を添加することによって製品の油臭さなどがマスキングされ、切れの良い風味となる。また、有機酸の増加により、生地が適度に緩むことが、生地伸展性の向上とそれに伴う機械耐性の向上につながるものと考えられる。 By adding the fermentation starter of the present invention, which contains a large amount of organic acids, especially lactic acid, the oily smell of the product is masked, resulting in a sharp flavor. In addition, the increase in organic acids causes the dough to loosen appropriately, which is thought to lead to improved dough extensibility and therefore improved mechanical resistance.

Figure 0007605584000002
Figure 0007605584000002

実験2.液状醗酵種を使用したパイ菓子の製造と評価
2-1.パイ菓子の製造
実験1で調製した醗酵種を使用してパイ菓子を製造した。小麦粉100質量%(強力粉:70質量%、薄力粉:30質量%)に対して、練込用油脂(チップ状またはストロー状油脂)60質量%、水50質量%、塩2.5質量%、液状醗酵種0~13質量%を添加し、常温下でミキシングしてパイ生地を作成した。
Experiment 2. Production and evaluation of pie confectionery using liquid fermented starter 2-1. Production of pie confectionery Pie confectionery was produced using the fermented starter prepared in Experiment 1. To 100% by mass of wheat flour (strong flour: 70% by mass, weak flour: 30% by mass), 60% by mass of kneading oil (chip-shaped or straw-shaped oil), 50% by mass of water, 2.5% by mass of salt, and 0 to 13% by mass of liquid fermented starter were added, and the mixture was mixed at room temperature to produce a pie dough.

このパイ生地を温度2℃で24時間リタード(低温保存)した。次いで、リタードした生地を12層に折り畳んで積層生地を作成した後、4mmの厚さに圧延してから、外径の直径が80mmになるようにリングドーナツ形状に切断した。 This pie dough was retarded (stored at low temperature) for 24 hours at a temperature of 2°C. The retarded dough was then folded into 12 layers to create a laminated dough, which was then rolled to a thickness of 4 mm and cut into a ring doughnut shape with an outer diameter of 80 mm.

続いて、リングドーナツ形状に切断した積層生地を温度200℃で20分間焼成した後、130℃で30分間焼成し、パイ菓子を製造した。 Next, the laminated dough cut into ring donut shapes was baked at 200°C for 20 minutes, and then baked at 130°C for 30 minutes to produce pie pastries.

Figure 0007605584000003
Figure 0007605584000003

2-2.パイ菓子の評価
それぞれのサンプルについて、製菓性(パイ生地加工適性)、焼色、形状(ボリュームや腰持ち)、内相、食感および風味を4段階で評価した(◎:大変良好、○:良好、△:やや劣る、×劣る)。食感および風味については、8名のパネラーが試食した上で合議により評価した。また、パイ菓子の高さについても測定した。
2-2. Evaluation of pie pastries Each sample was evaluated on a four-point scale for confectionery properties (suitability for pie pastry processing), baking color, shape (volume and firmness), internal structure, texture, and flavor (◎: very good, ○: good, △: slightly poor, ×: poor). Eight panelists tasted the samples and then evaluated the texture and flavor by consensus. The height of the pie pastries was also measured.

評価結果を下表に示すが、本発明に基づいて製造したパイ菓子はすべて、製造工程におけるパイ生地の伸展性、弾性等の状態が良好であり、製菓性(パイ生地の加工適性)に優れていた。また、本発明のパイ菓子は、形状や焼色が良好であり、優れた外観を有していた。さらに、本発明のパイ菓子は、内相も膜伸びがあって良好であった。さらにまた、本発明のパイ菓子は、食感が軽く、また風味もこくがあって、酸味がなくて良好であった。特に液状醗酵種を1~10質量%添加したときにはこれらの特徴がよく現れており、液状醗酵種を3~7質量%添加したときにはこれらの特徴が著しく現れていた
製菓性(パイ生地加工適性)は、1~10質量%の液状醗酵種を添加すると製造工程におけるパイ生地の伸展性、弾性等の性状が良好であり、特に3~7質量%の液状醗酵種を添加した場合、製菓性が著しく良好であった。これに対し、醗酵種を添加しないと生地の伸展性は劣り、また醗酵種を13質量%添加すると、生地荒れがひどく、不良であった。
The evaluation results are shown in the table below. All of the pie confectioneries produced according to the present invention had good extensibility, elasticity, and other properties of the pie dough during the production process, and were excellent in confectionery properties (suitability for processing of the pie dough). The pie confectioneries of the present invention also had good shape and browning, and had an excellent appearance. Furthermore, the inner phase of the pie confectioneries of the present invention had good membrane stretchability. Furthermore, the pie confectioneries of the present invention had a light texture and a rich flavor without a sour taste, which was good. In particular, these characteristics were well expressed when 1 to 10% by mass of liquid fermentation starter was added, and these characteristics were significantly expressed when 3 to 7% by mass of liquid fermentation starter was added. Confectionery properties (suitability for processing of pie dough) were good when 1 to 10% by mass of liquid fermentation starter was added, and properties such as extensibility and elasticity of the pie dough during the production process were good, and in particular, when 3 to 7% by mass of liquid fermentation starter was added, the confectionery properties were significantly good. In contrast, when no leavening agent was added, the dough had poor extensibility, and when 13% by mass of leavening agent was added, the dough became severely rough and was unsatisfactory.

外観・形状は、1~10質量%の液状醗酵種を添加すると焼色、ボリューム(容積)および高さが良好であり、特に3~7質量%の液状醗酵種を添加した場合、ボリューム(容積)および高さが著しく良好であった。これに対し、醗酵種を添加しないとコシもちが無く、また醗酵種を13質量%添加すると焼色が薄く、腰持ちが悪くてボリューム(容積)がなく、高さも醗酵種を添加しない場合よりもむしろ著しく低くなった。特に高さについては、醗酵種を添加しないと28.4mmであったのに対し、サンプル3(高さ:33.1mm)やサンプル4(高さ:33.2mm)は著しく高く、スチューデントのt検定では99%の信頼率で有意に高く、また、サンプル2(高さ:30.5mm)およびサンプル5(高さ:32.4mm)も著しく高く、スチューデントのt検定では95%の信頼率で有意に高かった。 In terms of appearance and shape, when 1-10% by mass of liquid fermented starter was added, the browning, volume, and height were good, and when 3-7% by mass of liquid fermented starter was added, the volume and height were particularly good. In contrast, when no fermented starter was added, the bread had no chewiness, and when 13% by mass of fermented starter was added, the browning was light, the bread had poor firmness, no volume, and the height was significantly lower than when no fermented starter was added. In particular, the height was 28.4 mm when no fermented starter was added, whereas sample 3 (height: 33.1 mm) and sample 4 (height: 33.2 mm) were significantly higher, and the Student's t-test showed that it was significantly higher at a 99% confidence level. Sample 2 (height: 30.5 mm) and sample 5 (height: 32.4 mm) were also significantly higher, and the Student's t-test showed that it was significantly higher at a 95% confidence level.

内相は、1~10質量%の液状醗酵種を添加すると膜伸びがあって良好であり、特に5~7質量%の液状醗酵種を添加した場合、著しく良好であった。これに対し、醗酵種を添加しないと内相の浮きが弱く、また醗酵種を13質量%添加すると、膜伸びがなく内相の目(気泡膜)が詰んでいて不良であった。 The internal phase had good film stretching when 1-10% by mass of liquid fermentation starter was added, and was particularly good when 5-7% by mass of liquid fermentation starter was added. In contrast, the internal phase did not float well when no fermentation starter was added, and when 13% by mass of fermentation starter was added, there was no film stretching and the internal phase's mesh (air bubble film) was clogged, resulting in poor results.

食感は、1~10質量%の液状醗酵種を添加すると軽くて良好であり、特に3~7質量%の液状醗酵種を添加した場合、著しく軽くて良好であった。これに対し、醗酵種を添加しないと重く、また醗酵種を13質量%添加すると、硬くて不良であった。 The texture was light and good when 1-10% by mass of liquid fermented starter was added, and was particularly light and good when 3-7% by mass of liquid fermented starter was added. In contrast, the texture was heavy when no fermented starter was added, and hard and poor when 13% by mass of fermented starter was added.

風味は、1~10質量%の液状醗酵種を添加するとコクがあり、酸味がなくて良好であり、特に3~7質量%の液状醗酵種を添加した場合、著しくコク味があり良好であった。これに対し、醗酵種を添加しないと風味が弱く、また醗酵種を13質量%添加すると、酸味が強くてコクが感じられずに不良であった。 The flavor was good, with plenty of body and no sourness, when 1-10% by mass of liquid fermented starter was added, and in particular, when 3-7% by mass of liquid fermented starter was added, it was extremely rich and good. In contrast, the flavor was weak when no fermented starter was added, and when 13% by mass of fermented starter was added, it was poor, with a strong sour taste and no body.

Figure 0007605584000004
Figure 0007605584000004

2-3.パイ菓子の機械的特性の評価
上記のようにして製造したパイ菓子について、その機械的特性を評価した。具体的には、パイ菓子に応力をかけ、パイ菓子を破断したときの破断荷重および面積を機械的に測定した。
2-3. Evaluation of mechanical properties of pie confectionery The mechanical properties of the pie confectionery produced as described above were evaluated. Specifically, stress was applied to the pie confectionery, and the breaking load and area when the pie confectionery was broken were mechanically measured.

測定条件は以下のとおりであった。
(測定装置) テクスチャーアナライザー
(プローブ) ブレードセットHDP/BS
(進入速度) 10mm/秒
(破断位置) 製品全体を破断
下表に評価結果を示すが、醗酵種を添加しない場合と比較して、醗酵種を1~10質量%添加すると、いずれも最大荷重値および面積値ともにかなり小さく、食感の軽さが著しいことが判った。特に醗酵種を7質量%添加した場合、最大荷重値および面積値は、醗酵種を添加しない場合に対し、スチューデントのt検定で99%の信頼率で有意に小さいことが判った。なお、醗酵種を13質量%添加したパイ菓子製品は生地荒れのため十分な量の検体が得られず、物性測定を行えなかった。
The measurement conditions were as follows:
(Measuring device) Texture analyzer (probe) Blade set HDP/BS
(penetration speed) 10 mm/sec (breakage position) Breaks entire product The evaluation results are shown in the table below. Compared to when no fermentation starter was added, when 1-10% fermentation starter was added, both the maximum load value and area value were significantly smaller, and the texture was significantly lighter. In particular, when 7% fermentation starter was added, the maximum load value and area value were found to be significantly smaller with a 99% confidence level in Student's t-test compared to when no fermentation starter was added. Note that for the pie confectionery product with 13% fermentation starter added, a sufficient amount of sample could not be obtained due to roughness of the dough, and physical properties could not be measured.

Figure 0007605584000005
Figure 0007605584000005

2-4.パイ菓子の嗜好性評価
醗酵種を5質量%添加したパイ菓子(実施品)と醗酵種を添加しないパイ菓子(対照品)について、嗜好性を詳細に評価した。訓練を受けた経験豊富な21人のパネラーにより官能評価を実施し、下記の基準に基づいて、7段階で評価した。数値が大きいほど、良好である。
・サックリ感(最初に噛んだ際に感じる食感の軽さ)
1点(まったくサックリしていない)←4点(どちらともいえない)→7点(非常にサックリしている)
・食感の軽さ(咀嚼全体で感じる軽さ)
1点(非常に重い)←4点(どちらともいえない)→7点(非常に軽い)
・口溶けの良さ
1点(まったく感じない)←4点(普通)→7点(非常に口溶けが良い)
パネラーの評点の平均値を下表に示すが、本発明の実施品は、サックリ感、食感の軽さおよび口溶けの各評価項目において優れた結果だった。すなわち、いずれの評価項目においても、本発明の実施品は、液状醗酵種を添加していないサンプルと比較して点数が高く、特にサックリ感、食感の軽さおよび口溶けの各評価項目においてはスチューデントのt検定で95%の信頼率で有意に点数が高かった。
2-4. Evaluation of the palatability of pie confectionery The palatability of pie confectionery to which 5% by mass of fermented yeast was added (product) and pie confectionery to which no fermented yeast was added (control product) was evaluated in detail. Sensory evaluation was carried out by 21 trained and experienced panelists, who rated the products on a 7-point scale based on the following criteria. The higher the value, the better the product.
・Crunchy texture (light texture felt when first biting)
1 point (not at all crisp) ← 4 points (can't say either way) → 7 points (very crisp)
- Lightness of texture (lightness felt throughout chewing)
1 point (very heavy) ← 4 points (neither heavy nor heavy) → 7 points (very light)
・Melting in the mouth: 1 point (not noticeable at all) ← 4 points (normal) → 7 points (very melting in the mouth)
The average scores of the panelists are shown in the table below, and the product of the present invention achieved excellent results in the evaluation items of crispiness, light texture, and melting in the mouth. In other words, in each evaluation item, the product of the present invention scored higher than the sample to which no liquid fermentation starter was added, and in particular, the scores in the evaluation items of crispiness, light texture, and melting in the mouth were significantly higher at a 95% confidence level using Student's t-test.

Figure 0007605584000006
Figure 0007605584000006

Claims (8)

小麦粉、水、乳酸菌および酵母菌を少なくとも含む原料を醗酵させて液状醗酵種を調製する工程と、
生地に使用する穀粉の全量に対して、0.5~12質量%の液状醗酵種を添加して生地を作成する工程と、
生地をイースト醗酵させないで焼成して焼き菓子を得る工程と、
を含み、前記酵母菌がサッカロミセス・シュバリエリを含む、焼き菓子の製造方法。
A step of fermenting a raw material containing at least wheat flour, water, lactic acid bacteria, and yeast to prepare a liquid fermented starter;
A step of preparing a dough by adding 0.5 to 12% by mass of a liquid fermentation starter to the total amount of flour used in the dough;
A step of baking the dough without yeast fermentation to obtain baked goods;
wherein the yeast comprises Saccharomyces chevalieri .
前記液状醗酵種に含まれる活性乳酸菌の菌数が、前記液状醗酵種に含まれる活性酵母菌の菌数よりも多い、請求項1に記載の方法。 The method according to claim 1, wherein the number of active lactic acid bacteria contained in the liquid fermentation agent is greater than the number of active yeast bacteria contained in the liquid fermentation agent. 前記液状醗酵種に含まれる活性乳酸菌の菌数が、前記液状醗酵種に含まれる活性酵母菌の菌数よりも10~10/g以上多い、請求項1または2に記載の方法。 3. The method according to claim 1, wherein the number of active lactic acid bacteria contained in said liquid fermentation agent is 10 8 to 10 9 /g or more higher than the number of active yeast bacteria contained in said liquid fermentation agent. 前記液状醗酵種に含まれる活性乳酸菌の菌数が10~1010/gであり、前記液状醗酵種に含まれる活性酵母菌の菌数が10~10/gである、請求項1~3のいずれかに記載の方法。 The method according to any one of claims 1 to 3, wherein the number of active lactic acid bacteria contained in said liquid fermentation agent is 10 6 to 10 10 /g, and the number of active yeast bacteria contained in said liquid fermentation agent is 10 5 to 10 9 /g. 前記液状醗酵種のpHが4以下である、請求項1~4のいずれかに記載の方法。 The method according to any one of claims 1 to 4, wherein the pH of the liquid fermentation mixture is 4 or less. 前記液状醗酵種の有機酸含有量が4000ppm以上である、請求項1~5のいずれかに記載の方法。 The method according to any one of claims 1 to 5, wherein the organic acid content of the liquid fermentation species is 4000 ppm or more. 前記液状醗酵種の乳酸含有量が3500ppm以上である、請求項1~6のいずれかに記載の方法。 The method according to any one of claims 1 to 6, wherein the lactic acid content of the liquid fermentation species is 3500 ppm or more. 前記焼き菓子がパイ菓子である、請求項1~7のいずれかに記載の方法。 The method according to any one of claims 1 to 7, wherein the baked confectionery is a pie confectionery.
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JP2004321097A (en) 2003-04-25 2004-11-18 Kanegafuchi Chem Ind Co Ltd Hops yeast species
JP2004357631A (en) 2003-06-06 2004-12-24 Kazuharu Inoue Cereal fermentation product and method for producing fermentation flavor confectionery by using the same
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