JP7647340B2 - Adhesive material sampling tool, adhesive material inspection kit, and adhesive material sampling method - Google Patents

Description

The present invention relates to a material collection tool, a material inspection kit, and a material collection method.

When analyzing substances attached to the surface of an object in sanitary tests, environmental measurements, quality tests of industrial products, etc., the analysis results are greatly affected by the sampling tools and sampling methods used to collect the substances. Therefore, various sampling tools and sampling methods have been proposed to achieve the recovery rate of the substances and the reproducibility of the measurements according to the purpose. The same is true for the collection of attached bacteria for testing attached bacteria on human skin.
A representative method for collecting adherent bacteria is the cup scrub method (see, for example, Non-Patent Document 1). This method is required by the American Society for Testing and Materials (ASTM), a standard method for evaluating antibacterial effects in the United States, when evaluating skin disinfection before surgery or before and after catheter insertion.

Specifically, in the cup scrub method, adherent bacteria are collected in the following manner.
(1) A glass or metal cylinder is pressed against the surface of a target object such as the skin.
(2) Inject the bacterial collection liquid into the cylinder.
(3) The surface of the object is scrubbed with a glass or metal rod for a certain period of time to disperse the bacteria into the bacteria collection liquid.
(4) A portion of the sample liquid in which the bacteria are dispersed is sucked up with a dropper and then dropped into the testing device.
This collection method is excellent in terms of recovery rate of adherent bacteria and reproducibility of measurements for the following reasons.
・Since the bacteria are dispersed in the bacterial collection liquid, a large amount of bacteria can be collected. By simply taking out a portion of the collection liquid in which the bacteria are dispersed, the detection limit of the testing device is exceeded, making it possible to measure the amount of bacteria.
・By making the inner diameter of each cylinder constant, it is possible to always scrub a constant area of skin. Therefore, when bacteria are collected on different days, it is possible to detect changes in the number of bacteria per unit area. In other words, quantitative measurement is possible.

Apart from the cup scrub method, a common method for collecting bacteria attached to the skin is the swab method. The swab method involves contacting the skin with a sampling stick soaked in a sampling liquid, and then rubbing the skin to collect bacteria (see, for example, Patent Documents 1 and 2).

Japanese Patent Application Publication No. 10-229871 JP 2011-69778 A

Journal of Healthcare-associated Infection, 3(2), 19-23, 2010

Collecting bacteria attached to the skin and testing their condition is also important for keeping the skin healthy. By using medicines and cosmetics appropriate to the state of the bacteria, the condition of the skin can be kept optimal. Therefore, it is desirable for individuals to be able to collect bacteria attached to the skin frequently in their daily lives.

However, when an individual collects bacteria adhering to the skin, the cup scrub method has the following problems when it is performed frequently on a daily basis.
To collect attached bacteria, it is necessary to inject the bacterial collection liquid into the cylinder while pressing the cylinder against the skin. If the cylinder is not pressed against the skin, the collection liquid poured from one side of the cylinder will spill out from the other side. This requires operation with both hands. Therefore, two people, an examiner and a test subject, are required to perform a skin attached bacteria test. For example, a person living alone cannot collect bacteria by himself in his own room.
Furthermore, with the cup scrub method, only a portion of the collected fluid is used for measurement by the testing device. The cylinder cannot be removed from the skin until all unused collected fluid has been removed from the cylinder. Recovering the collected fluid requires the use of a dropper, which takes time, and during that time the cylinder must be pressed against the skin, placing a heavy burden on both the test subject and the tester.
That is, the cup scrub method has the problem that it is difficult for one person to collect the deposits present on the surface of the object.

In addition, the swab method does not have a tool that determines the area to be sampled, such as the cylinder used in the cup scrub method. The skin is simply rubbed with a stick, and the area cannot be kept constant for each test. In addition, the sampled bacteria is squeezed out of the sampling stick and transferred to the testing device, but the amount of bacteria may vary depending on the squeezing technique.
Therefore, although it can detect the presence or absence of bacteria, it is not suitable for quantitative measurement such as whether the number of bacteria has increased or decreased compared to the previous measurement. In other words, the swab method has the problem that the area on the surface of the target object from which the attached matter is collected cannot be kept constant.

The present invention has been made in consideration of the above circumstances, and provides an attachment sampling tool, attachment inspection kit, and attachment sampling method that allow one person to sample attachments on the surface of an object while keeping the area of the portion of the object surface from which attachments are sampled constant.

The present invention provides the following attachment sampling device, attachment inspection kit, and attachment sampling method.

[1]
A sampling tool for sampling adhesions present on a surface of a target object,
A container body having an opening at one end and a closing portion at the other end;
a protrusion provided on at least one of the side wall portion of the container body and the closing portion;
Equipped with
The attachment sampling tool is configured so that the protrusions can rub the surface of the object when the container body is rotated in the circumferential direction while the opening side is brought into close contact with the surface of the object.
[2]
The deposit sampling tool described in the above-mentioned [1] satisfies the relationship S1 ≧ S2 × 0.5, where _S1 is the area of a path drawn by the surface of the opening side of the protrusion when the container body is rotated _once in the circumferential direction, _{and S2} is the area of the surface of the container body on the opening side.
[3]
The matter collecting tool according to [1] or [2], wherein the protrusion has a first protrusion that protrudes toward the internal space of the container body and is supported by the side wall portion, and a second protrusion that protrudes toward the side wall portion and is supported by the closing portion.
[4]
The deposit sampling tool according to any one of the above [1] to [3], wherein the surface of the side wall portion facing the opening and the surface of the protrusion portion facing the opening are substantially flush with each other.
[5]
The deposit sampling tool according to any one of the above [1] to [4], wherein the container body is cylindrical.
[6]
The deposit sampling tool according to any one of the above [1] to [5], wherein the container body is made of resin.
[7]
The deposit sampling tool according to any one of the above [1] to [6], further comprising a lid member for closing the opening.
[8]
The deposit sampling tool according to the above [7], wherein the cover member is an easily peelable film.
[9]
The material collection tool according to any one of the above [1] to [8], wherein the object is skin.
[10]
The deposit sampling tool according to any one of the above [1] to [9], wherein the deposit contains microorganisms.
[11]
The deposit collection tool according to any one of [1] to [10] above,
A collection liquid contained inside the container body of the deposit collection tool;
An adhesion inspection kit comprising:
[12]
A method for collecting adhesion material, comprising a step of rotating the container body in a circumferential direction while closely contacting the opening side of the adhesion material collecting tool according to any one of [1] to [10] above against a surface of a target object.

The present invention provides an attachment sampling tool, an attachment inspection kit, and an attachment sampling method that allow a single person to sample attachments on the surface of an object while keeping the area of the portion of the surface of the object from which the attachments are sampled constant.

1 is a schematic diagram showing an example of an attachment sampling tool according to the present invention, in which FIG. 1(1) is a top view, FIG. 1(2) is a side perspective view, and FIG. 1(3) is a bottom perspective view. ₁ is a schematic diagram for explaining the area S1 of the trajectory drawn by the surface on the opening side of the protrusion when the container body is rotated once in the circumferential direction, and the area _S2 of the surface on the opening side of the container body. FIG. FIG. 2 is a top view showing an example of a deposit collecting tool according to the present invention. FIG. 1 is a photograph showing an example of a deposit collection tool according to the present invention. FIG. 1 is a perspective view showing an example of an adhesion inspection kit according to the present invention. FIG. 1 is a schematic diagram showing an example of a procedure for a method for collecting and inspecting a substance using a substance inspection kit according to the present invention. FIG. 13 is a graph comparing the viable cell count of Staphylococcus epidermidis obtained by a method for inspecting adhesions using an adhesion sampling tool according to the present invention with the viable cell count of Staphylococcus epidermidis obtained by a cup scrub method.

Below, an embodiment of the present invention will be described with reference to the drawings. Note that in all drawings, similar components are given the same reference numerals and descriptions will be omitted as appropriate.

1. Adhesive Material Sampling Tool Fig. 1 is a schematic diagram showing an example of an adhesive material sampling tool according to the present invention, in which Fig. 1(1) is a top view, Fig. 1(2) is a side perspective view, and Fig. 1(3) is a bottom perspective view. Fig. 4 is a photograph showing an example of an adhesive material sampling tool according to the present invention.
The deposit sampling tool of the present invention is a sampling tool for sampling deposits present on the surface of an object, and comprises a container body having an opening c at one end and a closed portion f at the other end, and protrusions a, b on at least one of the side wall e and the closed portion f of the container body. In the deposit sampling tool of the present invention, the protrusions a, b are configured to be able to rub the surface of the object when the container body is rotated in the circumferential direction while the opening c side is in close contact with the surface of the object.
According to the deposit sampling tool of the present invention, the area of the object surface from which deposits are sampled can be kept constant, and sampling of deposits present on the object surface can be performed by one person.

The deposit sampling tool according to the present invention has a structure that combines a container body for holding a sampling liquid and a protrusion that serves as a scrubbing means, so that the collection of deposits can be performed with one hand. Therefore, the collection of deposits present on the surface of an object can be performed by one person (substantially with one hand).
In addition, in the deposit sampling tool according to the present invention, the area of the portion from which deposits are sampled is determined by the inner diameter of the surface of the container body on the opening c side (hereinafter also referred to as the "upper surface"). This allows the deposit sampling tool according to the present invention to keep the area of the portion from which deposits are sampled on the surface of the object constant, and allows deposits to be sampled from a substantially constant area.
In this specification, the upper surface refers to the surface on the open side of the container body, which faces the closing portion f (hereinafter also referred to as the "bottom plate").
Furthermore, the deposit collection tool of the present invention can improve the recovery rate of deposits by rubbing the projections of the container body against the skin to lift off deposits on the surface and disperse the deposits in the collection liquid.

As mentioned above, the cylinder in the cup scrub method must be pressed firmly against the skin with the hand holding it so that the collected liquid does not spill out from between the cylinder and the skin. At this time, the scrub stick is inserted from the open end of the cylinder, so the side wall of the cylinder is held. One end of the scrub stick must be held with the other hand, and the skin must be scrubbed evenly with the opposite end of the stick. For this reason, the cylinder must be of a certain size (thickness) for ease of use.
However, as mentioned above, the cylinder needs to be resistant to deformation because a strong force is applied to the cylinder when the hand holding the side wall presses it against the skin. To achieve this, the side wall needs to be thickened, which increases the manufacturing cost for frequent use. In addition, the scrubbing wand is often fitted with a cap made of silicon rubber or the like at the tip to scrub efficiently. Therefore, the manufacturing cost of the scrubbing wand is also high for frequent use.
On the other hand, in order to collect adhesions, the adhesion collection tool of the present invention only needs to be able to be pinched and rotated between the thumb and index finger. This allows for miniaturization. The side wall can also be reinforced with protrusions. This allows for a design using a resin with low strength. Therefore, the adhesion collection tool of the present invention can reduce the manufacturing cost of the collection tool, and can also reduce the price resistance to frequent collection of adhesions.

<Container body>
The container body has an opening c at one end, a closing portion f at the other end, a side wall portion e between the opening c and the closing portion f, and an internal space surrounded by the closing portion f and the side wall portion e and capable of holding liquid.
For example, one end of the container body is closed by a bottom plate to form a closed portion f, and the other end opposite the bottom plate is open to form an opening c.
The shape of the container body is not particularly limited, and may be cylindrical, square tube, or the like. From the viewpoint of facilitating rotation of the container body, a cylindrical shape is preferred.

The material of the container body is not particularly limited, and examples thereof include resin materials, metal materials, inorganic materials, and wood.
Among these, the material of the container body is preferably a resin material, because it has excellent heat sealability, moldability, oxidation resistance, sterilization resistance, recyclability, light weight, safety to the skin, etc., of the lid member described below. That is, the container body according to the present invention is preferably made of a resin.
Examples of the resin material include polyethylene, polypropylene, polystyrene, polyethylene terephthalate (PET), and polyvinyl chloride.

<Protrusion>
The protrusions a, b are supported by at least one of the side wall portion e and the closing portion f of the container body, and are configured to be able to rub the surface of the object when the container body is rotated in the circumferential direction while the opening c side is in close contact with the surface of the object.
In addition, it is preferable that the protrusions a and b are configured so as not to divide the internal space of the container body so that the collected liquid is not divided in the internal space of the container body, thereby making it possible to prevent adhesions from being omnipresent in the internal space of the container body.

In the deposit collection device of the present invention, when the area of the path traced by the surface on the opening side of the protrusion when the container body is rotated once in the circumferential direction is taken as _S1 and the area of the surface on the opening c side of the container body is taken as _S2 , from the viewpoint of improving the amount of deposit collection and the recovery rate, it is preferable to satisfy the relationship _S1 ≧ _S2 × 0.5, it is more preferable to satisfy the relationship _S1 ≧ _S2 × 0.8, it is even more preferable to satisfy the relationship _S1 ≧ _S2 × 0.9, it is even more preferable to satisfy the relationship _S1 ≧ _S2 × 0.95, and it is even more preferable to satisfy the relationship _S1 ≧ _S2 × 0.99.
Here, with reference to Figure 2, we will explain the area S1 of the trajectory drawn by the surfaces of the protrusions a and b on the opening c side when the container body is rotated _once in the circumferential direction, and the area _S2 of the surface of the container body on the opening c side.
First, area _S2 is the area of the surface of the container body on the opening c side, which is the area of the circle on the top surface of the cylindrical container in the case of Fig. 2. In other words, area _S2 is the sum of the area of the opening c, the area of the surfaces of the protrusions a and b on the opening c side, and the area of the surface d of the side wall e on the opening c side.
Moreover, area _S1 is the area of the path traced by the surfaces of protrusions a, b on the opening c side when the container body is rotated once in the circumferential direction, and in the case of Figure 2, it is the total area of area _S1a of the path traced by the surface of protrusion a on the opening c side and area _S1b of the path traced by the surface of protrusion b on the opening c side.

The protrusion portion according to the present invention is not particularly limited as long as it has a shape capable of rubbing the surface of an object. For example, as shown in FIG. 1, it is preferable for the protrusion portion to have a first protrusion portion b that protrudes toward the internal space of the container body and is supported by the side wall portion e, and a second protrusion portion a that protrudes toward the side wall portion e and is supported by the closing portion f.
In the example of Figure 1, the first protrusion b is supported by the side wall e and protrudes toward the internal space of the container body, and the second protrusion a is attached near the center of the bottom plate and protrudes toward the side wall e.
In addition, the first protrusion b provided on the side wall portion e acts as a beam to prevent deformation of the side wall portion e, so that the thickness of the side wall portion e can be made thinner by providing the first protrusion b.

FIG. 3 is a top view showing an example of the deposit collecting tool according to the present invention.
The protrusions according to the present invention may have any shape that allows them to rub the surface of an object, and may not be cross-shaped as shown in FIG. 1. For example, they may have any of the shapes shown in (1) to (4) in FIG.

In the crud collecting tool according to the present invention, as shown in FIG. 1, for example, it is preferable that the surface d of the side wall e on the opening c side and the surfaces of the projections a, b on the opening c side are substantially on the same plane.
That is, it is preferable that the surface (top surface) of the side wall portion e on the open end side and the surfaces (top surfaces) of the protrusions a, b on the open end side are substantially on the same plane. In this specification, "substantially on the same plane" refers to surfaces whose perpendicular distance between each other is, for example, 0.5 mm or less, preferably 0.3 mm or less, more preferably 0.1 mm or less.

The material of the protrusions is not particularly limited, and examples thereof include resin materials, metal materials, inorganic materials, and wood.
Among these, resin materials are preferred as the material for the protrusions because they are excellent in moldability, oxidation resistance, sterilization resistance, recyclability, light weight, safety to the skin, etc. That is, the protrusions according to the present invention are preferably made of resin.
Examples of the resin material include polyethylene, polypropylene, polystyrene, polyethylene terephthalate (PET), and polyvinyl chloride.
Furthermore, from the viewpoint of improving the moldability of the deposit sampling tool according to the present invention, the protrusion is preferably integrated with the container body, and more preferably is integrally molded with the container body using a resin material.

<Cover member>
The deposit sampling tool according to the present invention may further include a lid member g for closing the opening c. The lid member g is for closing the opening c of the container body and sealing the internal space of the container body. By pouring a sampling liquid into the internal space of the container body and sealing the opening c with the lid member, an deposit inspection kit, which will be described later, can be obtained.
The cover member g is not particularly limited, but for example, a resin film or a metal foil can be used, and it is preferable that the cover member g is an easily peelable film because it can be easily opened when used. As the easily peelable film, for example, a variety of films using a peeling mechanism such as interfacial peeling, cohesive peeling, and interlayer peeling are known, and can be appropriately selected from known films according to the material of the container body and the desired performance. In general, a laminate film having a polymer blend fusion layer on a base film, a laminate film having a hot melt fusion layer on a base film, an interface peeling laminate film having a seal layer or a peeling layer, etc. can be used.

＜Target Object＞
The subject from which the deposit collection tool of the present invention is used to collect deposits is not particularly limited, but examples include skin, mucous membrane, feces, food, clothing, daily necessities, soil, medical instruments, industrial products, etc. Among these, the deposit collection tool of the present invention is preferably used to collect deposits present on the skin surface. That is, the subject from which the deposit collection tool of the present invention is used to collect deposits is preferably skin.

＜Attachment＞
The deposits to be collected by the deposit collection tool of the present invention are not particularly limited, but examples include microorganisms such as bacteria, fungi, viruses, microalgae, protozoa, and mold; biological substances such as proteins, amino acids, lipids, nucleic acids, carbohydrates, vitamins, hormones, peptides, minerals, sebum, triglycerides, diglycerides, fatty acids, and glycerin; metals, polymers, various fine particles, and various foreign matter.
Among these, the deposit collection tool of the present invention can be suitably used for collecting microorganisms (preferably bacteria). That is, the deposits to be collected by the deposit collection tool of the present invention are preferably microorganisms, more preferably bacteria, and more preferably bacteria present on the skin surface.
Examples of the bacteria present on the skin surface include resident skin bacteria. Resident skin bacteria refers to bacteria that grow on human skin, but the species of the bacteria is not particularly limited in the present invention. Examples of the resident skin bacteria include Staphylococcus epidermidis, Staphylococcus aureus, Propionibacterium acnes, Corynebacterium, etc.

2. Adherent Material Inspection Kit Fig. 5 is a perspective view showing an example of an adhering material inspection kit according to the present invention.
The matter inspection kit according to the present invention comprises the matter sampling tool according to the present invention described above, and a sampling liquid h contained inside the container body of the matter sampling tool.
In the matter inspection kit according to the present invention, it is preferable that the matter sampling tool further comprises a cover member g for closing the opening c.
With this, the collected liquid h can be contained within the container body by storing the collected liquid h in the internal space of the container body and sealing the opening c with the lid member g, thereby improving the storability, handling, portability, etc. of the adhesion inspection kit before use.
The preferred embodiment of the cover member g is the same as the cover member described above.
The collection liquid h can be appropriately selected from known ones depending on the object and the attached matter. When the attached matter is bacteria, for example, physiological saline can be used as the collection liquid h.

3. Method for Sampling Adhesive Matter The method for sampling an adhesive matter using the adhesive matter sampling tool according to the present invention includes, for example, a step of rotating the container body in the circumferential direction while closely contacting the opening c side of the adhesive matter sampling tool according to the present invention with the surface of the object. For example, a sampling liquid is poured into the container body, and the container body is rotated in the circumferential direction while pressing the surface of the container body on the opening c side against the surface of the object, that is, the container body is rotated around the normal line of the upper surface as an axis. This causes the protrusions to scrub the surface of the object, dispersing the adhesive matter present on the surface of the object into the sampling liquid, and the adhesive matter can be sampled.

Hereinafter, an example of the procedure for sampling and testing bacteria present on the skin surface using the substance test kit according to the present invention will be described in more detail with reference to Figs. 5 and 6.
As shown in FIG. 5, the internal space of the container body of the adhesion inspection kit is filled with a sampling liquid h, and the upper surface is sealed with a lid member g such as an easily peelable film so that it can be easily opened.
First, the cover member g is peeled off from the container body to open the adhesion inspection kit (FIG. 6(1)).
Next, while being careful not to spill the collection liquid contained inside the container body, the surface of the container body on the opening c side is pressed against the area from which bacteria are to be collected and brought into close contact (FIG. 6(2)).
Next, as shown in Fig. 6 (3), the container is turned upside down so that the collection liquid touches the skin. In this state, the container body is rotated in the circumferential direction while pressing the surface of the opening c of the container body against the skin surface. This operation causes the protrusions of the attachment collection tool to move through the collection liquid while touching the skin surface, and to stir up the bacteria adhering to the skin surface into the collection liquid. The movement of the protrusions in the collection liquid further stirs the liquid, dispersing the bacteria that have been stirred up from the skin surface into the collection liquid.
Next, the container is turned upside down again to return it to the state shown in FIG. 6(2), and the container body is removed from the skin, taking care not to spill the collected liquid inside.
Next, the sample liquid in which the bacteria are dispersed within the container body is sucked up with a dropper or the like (FIG. 6 (4)).
Next, the collected liquid is transferred to a testing device (testing tool) and measured (Figure 6 (5)).
The method for testing the collected liquid containing the attached matter can be appropriately selected from among known testing methods depending on the object and the attached matter.
In addition, the above describes an example of using an adhesion inspection kit in which the adhesion sampling device according to the present invention is filled with sampling liquid and sealed with a lid member, but a method in which the subject himself/herself pours the sampling liquid from a separately stored container into the sampling device and then uses the sampling device may also be used.

The present invention will be specifically explained below with reference to examples, but the present invention is not limited to these examples in any way.

Example 1
A 3D printer was used to create the sampling tool (bottom area 1 ^cm2 ) shown in Figure 4. The obtained sampling tool was sterilized, and 1 mL of the sterilized bacterial sampling solution was placed in the sampling tool. As the sampling solution, physiological saline containing polyoxyethylene (20) sorbitan monolaurate (manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) as a surfactant was used.
Here, the skin surface is covered with sebum, which cannot be washed off effectively with water, so a surfactant is added to the collection solution in order to dissolve the sebum in the collection solution. Note that it was confirmed in advance that the surfactant concentration used in the experiment does not lyse Staphylococcus epidermidis.
In addition, physiological saline is used to prevent bacteria from dying due to the sudden change in osmotic pressure when they move from the skin surface, which has a high salt concentration, to the collection solution, which has a low salt concentration.

Next, the sampling tool was pressed against the cheek while being careful not to spill the sampling liquid, the angle was adjusted so that the skin surface was evenly wetted with the sampling liquid, and the tool was rotated several times using the thumb and index finger around the axis normal to the contact surface. After removing the sampling tool from the skin surface while being careful not to spill the sampling liquid, the sampling liquid was collected using a micropipette, and the number of S. epidermidis colonies was measured using the plate dilution method.
As a result, the bacterial count was 3.63±0.77 Log CFU/ ^cm2 (n=3), which was slightly higher than the count of Staphylococcus epidermidis obtained from the cheeks of 80 healthy women by the cup scrub method (2.77±0.98 Log CFU/ ^cm2 ) (J. Soc. Cosmet. Chem. Jpn. 28 (1) 44-56 (1994)) (see FIG. 7).
From the above, it was found that the use of the sampling tool of the present invention can provide a high bacterial sampling rate that is comparable to that of the cup scrub method.

a Projection (second projection)
b Projection (first projection)
c Opening d Opening side surface of side wall e Side wall f Closure g Lid member h Collected liquid

Claims (11)

A sampling tool for sampling adhesions present on a surface of a target object,
A container body having an opening at one end and a closing portion at the other end;
a protrusion provided on at least one of the side wall portion of the container body and the closing portion;
Equipped with
The protrusion protrudes toward an internal space of the container body,
a surface of the side wall portion facing the opening and a surface of the protrusion portion facing the opening are substantially flush with each other;
The attachment sampling tool is configured so that the protrusions can rub the surface of the object when the container body is rotated in the circumferential direction while the opening side is brought into close contact with the surface of the object. 2. The material collecting tool according to claim 1, wherein when the area of a path drawn by the surface of the opening side of the protrusion when the container body is rotated once in the circumferential direction is _S1 and the area of the surface of the container body on the opening side is _S2 , the relationship _S1 ≧ _S2 × 0.5 is satisfied. The attachment collector according to claim 1 or 2, wherein the protrusion has a first protrusion that protrudes toward the internal space of the container body and is supported by the side wall, and a second protrusion that protrudes toward the side wall and is supported by the closing portion. The deposit collection tool according to claim 1 or 2, wherein the container body is cylindrical. The deposit collection tool according to claim 1 or 2, wherein the container body is made of resin. The attachment collection tool according to claim 1 or 2, further comprising a lid member for closing the opening. 7. The material collecting tool according to claim 6 , wherein the cover member is an easily peelable film. The attachment collection tool according to claim 1 or 2, wherein the object is skin. The deposit collector according to claim 1 or 2, wherein the deposit includes microorganisms. The deposit collector according to claim 1 or 2,
A collection liquid contained inside the container body of the deposit collection tool;
An adhesion inspection kit comprising: A method for collecting attachments, comprising a step of rotating the container body in the circumferential direction while closely contacting the opening side of the attachment collection tool described in claim 1 or 2 with the surface of the object.