JP7666933B2 - Cell Culture Systems - Google Patents

Description

The present invention relates to a system for culturing cells.

Conventionally, there is a cell culture system that controls the concentration of a target gas in a culture solution based on the dissolved gas concentration detected by a dissolved gas sensor, and controls the culture solution delivery rate by a delivery pump based on the flow rate of the culture solution measured by a flow rate sensor (see Patent Document 1). The cell culture system described in Patent Document 1 is said to be able to further improve the accuracy of control of the dissolved gas concentration.

JP 2020-89304 A

Incidentally, the cell culture system described in Patent Document 1 takes into consideration the accuracy of control of the dissolved gas concentration, but does not consider the risk of damage to cell tissue due to the flow of the culture solution. In response to this, it is possible to reduce the flow rate of the culture solution to prevent cell damage, but if the flow rate of the culture solution is made too low, the preparation process before starting cell culture will take a long time.

The present invention has been made to solve the above problems, and its main objective is to provide a cell culture system that can reduce the time required for the preparation process before starting cell culture while suppressing damage to cell tissue caused by the flow of culture medium.

A first means for solving the above problem is a cell culture system,
A culture tank for storing a culture solution;
A supply pipe for supplying the culture solution from the outside to the inside of the culture tank;
A flow rate adjusting unit that adjusts the flow rate of the culture solution flowing through the supply pipe;
A pressure sensor for detecting a pressure of the culture solution supplied to the culture tank through the supply pipe;
A control unit that executes a first mode in which the flow rate adjustment unit is controlled to supply the culture solution to the culture tank through the supply pipe at a first flow rate, and a second mode in which the pressure detected by the pressure sensor after the end of the first mode is lowered below a predetermined damage pressure at which a predetermined cell tissue begins to be damaged, and the culture solution is supplied to the culture tank through the supply pipe at a second flow rate smaller than the first flow rate,
Equipped with.

According to the above configuration, the culture tank stores culture liquid. The supply pipe supplies the culture liquid from the outside to the inside of the culture tank. The flow rate adjustment unit adjusts the flow rate of the culture liquid flowing through the supply pipe. The pressure sensor detects the pressure of the culture liquid supplied to the culture tank by the supply pipe. Therefore, when culture liquid is supplied to the inside of the culture tank by the supply pipe, the flow rate of the culture liquid flowing through the supply pipe can be adjusted by the flow rate adjustment unit while the pressure sensor detects the pressure of the culture liquid supplied to the culture tank.

Here, the control unit executes a first mode in which the flow rate adjustment unit is controlled to supply the culture solution to the culture tank at a first flow rate through the supply pipe. Therefore, the first mode is executed before the cell tissue is stored in the culture tank, i.e., in a state where there is no risk of damaging the cell tissue, and the culture solution can be supplied to the culture tank at the first flow rate through the supply pipe. Therefore, the time required for the preparation process before starting cell culture (for example, the process of storing the culture solution in the culture tank with the adjusted dissolved gas concentration) can be shortened.

The control unit then executes a second mode in which the pressure detected by the pressure sensor after the end of the first mode is lowered below a predetermined damage pressure at which the predetermined cell tissue begins to be damaged, and the flow rate adjustment unit is controlled to supply the culture fluid to the culture tank through the supply pipe at a second flow rate smaller than the first flow rate. Therefore, the second mode is executed after the cell tissue is stored in the culture tank, the pressure of the culture fluid supplied to the culture tank through the supply pipe is detected by the pressure sensor, and the pressure of the culture fluid is lowered below the damage pressure and the flow rate of the culture fluid is set to a second flow rate smaller than the first flow rate, thereby preventing the predetermined cell tissue from being damaged by the flow of the culture fluid. Moreover, by executing the second mode after the first mode, the preparation process before starting cell culture and the cell culture process can be easily executed, and the effort of the user who executes cell tissue culture can be reduced.

The second method includes a cell storage section for storing the cell tissue, the cell storage section is stored inside the culture tank, and the supply pipe is inserted from the outside to the inside of the culture tank and connected to the cell storage section.

According to the above configuration, the cell storage unit stores the cell tissue. The cell storage unit is stored inside the culture tank. Therefore, by placing the cell storage unit storing the specified cell tissue in the culture tank, the cell tissue can be easily placed in the culture tank.

Here, the supply pipe is inserted from the outside to the inside of the culture tank and connected to the cell storage section. This allows the culture medium to be directly supplied to the inside of the cell storage section, making it easier to generate a flow of culture medium inside the cellular tissue stored in the cell storage section. This makes it easier to culture the cellular tissue in an appropriate environment where a flow of culture medium is generated inside the cellular tissue, and the cultivation of the cellular tissue can be promoted. Moreover, since the pressure detected by the pressure sensor is set lower than a predetermined damage pressure at which a predetermined cellular tissue begins to be damaged, damage to the cellular tissue due to the flow of culture medium can be suppressed.

In the third means, the pressure sensor detects the pressure of the culture medium inside the cellular tissue that is supplied to the culture tank through the supply pipe. With this configuration, the pressure sensor can detect the pressure of the culture medium inside the cellular tissue, which further prevents the cellular tissue from being damaged by the flow of the culture medium. In addition, the pressure of the culture medium inside the cellular tissue as the environment in which the cellular tissue is cultured can be more accurately grasped.

The fourth means includes a pressure receiving unit that receives input of a set pressure, and the control unit executes a pressure control mode in the second mode in which the flow rate adjustment unit is controlled so that the pressure detected by the pressure sensor becomes the set pressure received by the pressure receiving unit.

According to the above configuration, the pressure receiving unit receives the input of the set pressure. Therefore, the pressure of the culture fluid in the cell culture process can be set arbitrarily. However, the set pressure must be set lower than the damage pressure and the flow rate of the culture fluid must be set to a second flow rate that is lower than the first flow rate. Then, the control unit executes, as the second mode, a pressure control mode in which the flow rate adjustment unit is controlled so that the pressure detected by the pressure sensor becomes the set pressure received by the pressure receiving unit. Therefore, by executing the pressure control mode as the second mode, cell culture can be performed in a state in which the pressure detected by the pressure sensor is maintained at the set pressure.

The fifth means includes a flow rate receiving unit that receives input of a set flow rate, and a flow rate sensor that detects the flow rate of the culture medium supplied to the culture tank through the supply pipe, and the control unit executes, as the second mode, a flow rate control mode in which the flow rate control unit is controlled to make the flow rate detected by the flow rate sensor equal to the set flow rate received by the flow rate receiving unit.

According to the above configuration, the flow rate receiving unit receives input of a set flow rate. Therefore, the flow rate of the culture medium in the cell culture process can be set arbitrarily. However, the set flow rate needs to be set smaller than the first flow rate. Then, the control unit executes a flow rate control mode as the second mode in which the flow rate detected by the flow rate sensor is controlled to the set flow rate received by the flow rate receiving unit. Therefore, by executing the flow rate control mode as the second mode, cell culture can be performed in a state in which the flow rate detected by the flow rate sensor is maintained at the set flow rate.

The sixth means includes a pressure receiving unit that receives input of a set pressure, a flow rate receiving unit that receives input of a set flow rate, and a flow rate sensor that detects the flow rate of the culture medium supplied to the culture tank through the supply piping, and the control unit selectively executes, as the second mode, a pressure control mode in which the flow rate adjustment unit is controlled to make the pressure detected by the pressure sensor the set pressure received by the pressure receiving unit, and a flow rate control mode in which the flow rate adjustment unit is controlled to make the flow rate detected by the flow sensor the set flow rate received by the flow rate receiving unit.

According to the above configuration, the control unit can selectively execute the pressure control mode of the fourth means and the flow rate control mode of the fifth means. Therefore, it is possible to select and execute a mode suitable for culturing depending on the purpose and type of cell tissue.

In the seventh method, the first flow rate is greater than five times the second flow rate. Therefore, the first flow rate of the culture medium in the first mode can be greater than five times the second flow rate of the culture medium in the second mode, and the time required for the preparation process can be significantly reduced.

The first mode is basically performed before the cell tissue is stored in the culture tank, i.e., when there is no risk of damaging the cell tissue. However, if the pressure of the culture medium in the first mode is higher than the damage pressure, there is a risk that culture medium at a pressure higher than the damage pressure will temporarily flow into the cell tissue when switching to the second mode.

In this regard, in the eighth means, the control unit makes the pressure detected by the pressure sensor lower than the damage pressure in the first mode. Therefore, when switching from the first mode to the second mode, it is possible to prevent culture medium at a pressure higher than the damage pressure from temporarily flowing into the cell tissue.

The ninth means includes a state sensor that detects the state of the culture medium, a state adjustment unit that adjusts the state of the culture medium, and a display unit that displays an image, and the control unit controls the state adjustment unit in the first mode to make the state detected by the state sensor a target state, and causes the display unit to display an image indicating that the preparation process has been completed, on condition that the state detected by the state sensor has become the target state.

According to the above configuration, the state sensor detects the state of the culture liquid. As the state of the culture liquid, for example, at least one of the pH, dissolved oxygen concentration DO, temperature T, etc. of the culture liquid can be adopted. The state adjustment unit adjusts the state of the culture liquid. For example, the state adjustment unit can adjust the pH by dissolving carbon dioxide in the culture liquid, adjust the dissolved oxygen concentration DO by dissolving oxygen in the culture liquid, and adjust the temperature T by heating the culture liquid with a heater. The display unit displays an image.

In the first mode, the control unit controls the condition adjustment unit to make the state detected by the state sensor a target state. Therefore, by executing the first mode, the actual state of the culture medium can be made the target state. Then, the control unit causes the display unit to display an image indicating that the preparation process is complete, on the condition that the state detected by the state sensor has become the target state. Therefore, the user can easily know that the preparation process is complete by confirming that the display unit has displayed an image indicating that the preparation process is complete. In addition, the user can easily know that the preparation process is not complete by confirming that the display unit has not displayed an image indicating that the preparation process is complete.

The tenth means includes a confirmation receiving unit that receives an operation that confirms that the preparation process has been completed, and when the control unit receives the operation from the confirmation receiving unit, the control unit stops the supply of the culture medium to the culture tank through the supply pipe.

According to the above configuration, the confirmation receiving unit receives an operation that confirms that the preparation process has been completed. Then, when the control unit receives the operation from the confirmation receiving unit, the control unit stops the supply of the culture medium to the culture tank through the supply pipe. Therefore, when a user performs an operation that confirms that the preparation process has been completed, the supply of culture medium to the culture tank can be stopped, and the process can safely proceed to the process of storing cell tissue in the culture tank.

In the eleventh means, the control unit causes the display unit to display an image indicating that the preparation process has been completed, and then controls the condition adjustment unit to maintain the state detected by the condition sensor at the target state until the operation is received by the confirmation receiving unit. With this configuration, the state of the culture fluid can be maintained at the target state after the preparation process is completed, until the user performs an operation that confirms that the preparation process has been completed. Therefore, the user can start cell culture at the time desired by performing the operation that confirms that the preparation process has been completed.

In the twelfth means, the state of the culture medium includes the pH, dissolved oxygen concentration, and temperature of the culture medium. With this configuration, the pH, dissolved oxygen concentration DO, and temperature T of the culture medium can be set to the target pH, target dissolved oxygen concentration, and target temperature, respectively, while the first mode is being executed. Therefore, the process can be quickly transitioned to the cell culture process after the first mode is completed, reducing the user's effort.

In the thirteenth means, the control unit stops the flow rate adjustment unit when the flow rate detected by the flow rate sensor is an abnormal flow rate. With this configuration, the flow of culture medium can be stopped when the culture medium cannot be circulated normally. Therefore, the burden on the user to monitor the cell culture system during the first and second modes can be reduced.

In the fourteenth means, the control unit stops the condition adjustment unit when the condition detected by the condition sensor is an abnormal condition. With this configuration, the adjustment of the culture fluid condition can be stopped when the culture fluid condition cannot be adjusted normally. Therefore, the burden on the user to monitor the cell culture system during the first and second modes can be reduced.

FIG. 1 is a schematic diagram showing a cell culture system. 11 is a flowchart showing a procedure in a standby mode. 11 is a flow chart showing the procedure of a culture mode. 11 is a flowchart showing a procedure for liquid transfer control.

Below, an embodiment of a cell culture system for culturing cells will be described with reference to the drawings.

As shown in FIG. 1, the cell culture system 10 includes a pressure-controlled liquid delivery unit 20, a cell culture tank 50, a mixer 60, mass flow controllers (MFCs) 61-63, a control unit 45, a display unit 70, a pump 90, etc.

The pressure-controlled liquid delivery unit 20 (flow rate adjustment unit) includes an air supply source 21, a flow rate proportional valve 24, a throttle valve 27, a sterilizing filter 31, a tank 34, a pressure sensor 37, a liquid level sensor 41, a flow rate sensor 85, etc.

The air supply source 21 (gas supply source) supplies air (gas) at a predetermined pressure. The air supply source 21 is connected to the tank 34 by pipes 22 and 23. The pipe 22 is provided with a flow proportional valve 24. The flow proportional valve 24 can adjust its opening in proportion to an input signal, and adjusts the flow rate of air supplied from the air supply source 21. The flow proportional valve 24 is controlled by the control unit 45.

Pipe 22 and pipe 23 are connected at connection part 25. Pipe 23 is provided with a sterilizing filter 31. Sterilizing filter 31 sterilizes the air flowing to tank 34 via pipe 23. Tank 34 is formed in a cylindrical shape with a bottom, is airtight, and stores (contains) culture liquid L. Pipe 23 is connected to the top of tank 34. In other words, pipe 23 is connected to a position in tank 34 that is higher than the liquid level of culture liquid L, and is not in contact with culture liquid L. The pipes 22 and 23 form a first pipe. The shape of tank 34 is not limited to a cylindrical shape with a bottom, and may be any shape.

To the culture solution L, a reagent such as bicarbonate that is free and has a pH buffering function in the pH range of 6 to 8 is added. As the bicarbonate, sodium bicarbonate (sodium hydrogen carbonate), potassium hydrogen carbonate, calcium hydrogen carbonate, etc. can be used. In addition, serum, amino acids, vitamins, sugars, etc. are added to the culture solution L as nutrients necessary for the activity of the cellular tissue B (cells) that has blood vessels. An indicator such as phenol red is added to the culture solution L to visually check the pH.

The pressure sensor 37 detects the pressure P1 of the culture solution L at the bottom of the tank 34. The liquid level sensor 41 detects the liquid level position of the culture solution L in the tank 34.

A pipe 26 is connected to the connection part 25. The connection part 25 is connected to an air exhaust part 28 by the pipe 26. A throttle valve 27 is provided in the pipe 26. The throttle valve 27 (throttle part) is, for example, a needle valve that adjusts a minute flow rate, and limits (adjusts) the flow rate of air exhausted to the exhaust part 28. The opening degree of the throttle valve 27 is set to a predetermined opening degree so that the pressure of the air in the tank 34 (pressure in the tank 34) becomes the target pressure. The opening degree of the throttle valve 27 may be changed according to the target pressure of the air in the tank 34. The air that flows to the exhaust part 28 is exhausted to the outside (atmosphere) of the pressure control liquid delivery part 20. The pipes 23 and 26 constitute a second pipe.

The cell culture tank 50 (culture tank) is formed in a hollow rectangular parallelepiped shape and stores (contains) the culture liquid L inside. Note that the shape of the cell culture tank 50 is not limited to a hollow rectangular parallelepiped shape, and may be a hollow cylindrical shape, etc.

A cell storage section 51 is installed (stored) inside the cell culture tank 50. The cell storage section 51 is formed in a hollow rectangular parallelepiped shape without an upper bottom. The above-mentioned cell tissue B is stored inside the cell storage section 51. Note that the shape of the cell storage section 51 is not limited to a hollow rectangular parallelepiped shape without an upper bottom, and may be a hollow cylindrical shape without an upper bottom, etc.

The liquid supply pipes 42A and 42B (supply pipes) connect the culture liquid L stored in the tank 34 to the cell storage section 51 (cell culture tank 50). That is, the liquid supply pipes 42A and 42B supply the culture liquid L from the outside to the inside of the cell culture tank 50. The liquid supply pipe 42A is inserted into the culture liquid L stored in the tank 34, and the lower end of the liquid supply pipe 42 is located lower than the liquid level of the culture liquid L in the tank 34.

The liquid supply pipes 42A and 42B are provided with an electromagnetic valve 46, a check valve 47, a flow rate sensor 85, etc. The electromagnetic valve 46 opens and closes the liquid supply pipe 42A. The electromagnetic valve 46 is controlled by the control unit 45. The check valve 47 (non-return valve) allows the culture liquid L to flow from the electromagnetic valve 46 (tank 34) to the cell storage unit 51 (cell culture tank 50) and prohibits the culture liquid L from the cell storage unit 51 (cell culture tank 50) to the electromagnetic valve 46 (tank 34). The flow rate sensor 85 detects the flow rate of the culture liquid L flowing through the liquid supply pipe 42A, i.e., the flow rate of the culture liquid L supplied to the cell culture tank 50 by the liquid supply pipes 42A and 42B.

Culture fluid L is delivered (supplied) to the cell culture tank 50 from the tank 34 via the delivery pipes 42A and 42B. More specifically, the culture fluid L is delivered directly to the cell storage unit 51 via the delivery pipe 42B. This forms a flow path Bp for the culture fluid L in the cell tissue B, causing a flow of the culture fluid L in the cell tissue B. The culture fluid L that flows through the flow path Bp flows out to the outside of the cell storage unit 51 as shown by the dashed arrow. The cell tissue B is, for example, an animal cell tissue having blood vessels. The cell tissue B is cultured with the culture fluid L and develops a predetermined biological function. At that time, the dissolved oxygen concentration DO and pH of the culture fluid L in the cell culture tank 50 change due to the activity of the cells.

During the cultivation of the cell tissue B, the culture fluid L is fed to the cell storage section 51 of the cell culture tank 50 at a pressure (pressurization) lower than the damage pressure Pd [mmHg] so as not to damage the blood vessels of the cell tissue B. The damage pressure Pd is a predetermined pressure (for example, 5 to 10 [mmHg]) at which the cell tissue B begins to be damaged, and varies depending on the type of cell tissue B. In addition, in order for the cell tissue B to express a predetermined biological function, it is desirable to use pressure-controlled feeding, which feeds the culture fluid L at a target pressure, rather than flow-controlled feeding, which feeds the culture fluid L at a target flow rate. The reason for this is that in the animal cell tissue of an actual animal, blood is fed by the heart, which is closer to pressure-controlled feeding than flow-controlled feeding. Furthermore, in order for the cell tissue B to express a predetermined biological function, it is desirable for the pressure acting on the cell tissue B to be stable. Note that when the culture fluid L is fed to the cell storage section 51 at a pressure (pressurization) lower than the damage pressure Pd [mmHg], the flow rate of the culture fluid L is smaller than the culture flow rate Fg [mL/min]. The culture flow rate Fg (second flow rate) is a flow rate suitable for culturing cell tissue B, for example, 1 to 3 mL/min.

An oxygen concentration sensor 81, a pH sensor 82, and a temperature sensor 83 are inserted into the culture fluid L stored in the cell culture tank 50. The oxygen concentration sensor 81 (status sensor) detects the dissolved oxygen concentration DO (status) of the culture fluid L in the cell culture tank 50. The pH sensor 82 (status sensor) detects the pH (status) of the culture fluid L in the cell culture tank 50. When detecting the pH of the culture fluid L by the pH sensor 82, a small amount of reference liquid (internal liquid) may leak from the pH sensor 82 into the culture fluid L. The temperature sensor 83 (status sensor) detects the temperature (status) of the culture fluid L in the cell culture tank 50. In addition, a pressure sensor 84 is inserted into the flow path Bp (cell tissue B) of the cell tissue B. The pressure sensor 84 detects the pressure of the culture fluid L in the flow path Bp, i.e., the pressure of the culture fluid L supplied to the cell storage section 51 (cell culture tank 50) by the liquid supply pipe 42B.

The cell culture tank 50 is provided with a heater 52. The heater 52 (condition adjustment unit) heats the culture solution L stored in the cell culture tank 50. The heater 52 is controlled by the control unit 45. The culture solution L inside the cell culture tank 50 is stirred by a stirrer (not shown).

The cell culture tank 50 is connected to the tank 34 by the return liquid pipe 43. The return liquid pipe 43 is provided with a pump 90 and a check valve 92. The pump 90 pumps the culture liquid L when the liquid level detected by the liquid level sensor 41 falls below a predetermined position (liquid level sensor 41: OFF). The pump 90 stops pumping the culture liquid L when the liquid level detected by the liquid level sensor 41 rises above the predetermined position (liquid level sensor 41: ON). The check valve 92 (non-return valve) allows the culture liquid L to flow from the pump 90 to the tank 34 and prohibits the culture liquid L from flowing from the tank 34 to the pump 90. The return liquid pipe 43 is connected to a position lower than the liquid level of the culture liquid L in the tank 34 and higher than the lower end of the liquid delivery pipe 42A.

The control unit 45 is a microcomputer equipped with a CPU, ROM, RAM, an input/output interface, etc. The control unit 45 controls the flow proportional valve 24 to send the culture fluid L to the cell culture tank 50 at the target pressure. In other words, the pressure control fluid sending unit 20 adjusts the flow rate of the culture fluid L flowing through the fluid sending pipes 42A and 42B.

When the control unit 45 controls the pressure control liquid delivery unit 20 to deliver the culture liquid L from the tank 34 to the cell culture tank 50 via the liquid delivery pipes 42A and 42B, it controls the flow proportional valve 24 so that air is supplied at a target flow rate calculated based on predetermined conditions. The control unit 45 maintains the state for a predetermined time (e.g., 3 [S]) of 5 [S] or less until the pressure Pv in the tank 34 is equilibrated. In this embodiment, the pressure Pv is equilibrated in approximately 1 [S]. As a result, the culture liquid L is delivered to the cell culture tank 50 at the target pressure.

The time from when air starts to be supplied from the air supply source 21 until the pressure Pv inside the tank 34 is equilibrated is longer the larger the volume of the tank 34 is, the larger the effective cross-sectional area S of the throttle valve 27 is, and the higher the target pressure inside the tank 34 is. In this regard, in culturing cell tissue B having blood vessels, it is necessary to send the culture fluid L to the cell culture tank 50 at a minute pressure lower than the damage pressure Pd [mmHg], so the target pressure inside the tank 34 is set very low. Therefore, the time until the pressure Pv is equilibrated is very short.

When the culture solution L is sent from the tank 34 to the cell culture tank 50 through the liquid sending pipes 42A and 42B, the control unit 45 balances the pressure inside the tank 34 in a state where the flow rate proportional valve 24 is controlled so that air is supplied at a target flow rate corresponding to the target pressure inside the tank 34 based on a predetermined correlation between the flow rate of air supplied from the air supply source 21 in a state where the pressure inside the tank 34 is balanced and the pressure inside the tank 34. In other words, the control unit 45 balances the pressure inside the tank 34 in a state where the flow rate proportional valve 24 is controlled so that air is supplied at a target flow rate corresponding to the target pressure inside the tank 34 based on a predetermined correlation between the flow rate of air supplied from the air supply source 21 in a state where the pressure inside the tank 34 is balanced and the pressure inside the tank 34, and sends the culture solution L from the tank 34 to the cell culture tank 50 through the liquid sending pipes 42A and 42B.

Oxygen, carbon dioxide, and nitrogen supplied from an oxygen cylinder (not shown), a carbon dioxide cylinder (not shown), and a nitrogen cylinder (not shown) are supplied to the mixer 60 via MFCs 61-63, respectively. The MFCs 61-63 (condition adjustment units) adjust the flow rates of oxygen, carbon dioxide, and nitrogen, respectively. The MFCs 61-63 are controlled by the control unit 45. The mixer 60 mixes these gases. The mixer 60 is connected to the cell culture tank 50. The oxygen, carbon dioxide, and nitrogen mixed in the mixer 60 are supplied to the cell culture tank 50 via gas piping 65. The gas supplied to the cell culture tank 50 is discharged to the outside of the cell culture tank 50 from a gas outlet or gap in the cell culture tank 50.

The display unit 70 is composed of a touch panel (liquid crystal panel + touch pad). The display unit 70 (pressure receiving unit, flow rate receiving unit, confirmation receiving unit) displays images and receives touch operations by the user. Touch operations include pressing a displayed button (icon), inputting numerical values, etc.

In the cell culture system 10 described above, a preparation process must be performed before starting the culture process of the cell tissue B. In the preparation process, for example, culture solution L with an adjusted dissolved gas concentration and temperature T is stored in the cell culture tank 50. The cell culture system 10 performs the preparation process in standby mode and performs the culture process in culture mode.

In the standby mode (first mode), the control unit 45 controls the pressure-controlled liquid delivery unit 20 to supply the culture fluid L to the cell culture tank 50 at a first flow rate F1 through the liquid delivery pipes 42A and 42B. In detail, the user operates the image displayed in advance by the display unit 70 to input a set pressure P1s, which is a target value of the pressure P1 detected by the pressure sensor 37 in the standby mode. The set pressure P1s is a pressure lower than the damage pressure Pd and is a pressure at which the culture fluid L can be delivered quickly to the cell culture tank 50. The display unit 70 also accepts an operation to start the standby mode by the user. The display unit 70 displays a "standby" button that instructs the start of the standby mode. The control unit 45 starts the standby mode when the "standby" button is pressed.

In the culture mode (second mode), the control unit 45 controls the pressure control liquid delivery unit 20 to lower the pressure P2 detected by the pressure sensor 84 below the damage pressure Pd after the standby mode ends and to supply the culture liquid L to the cell culture tank 50 at a second flow rate F2 lower than the first flow rate F1 through the liquid delivery pipes 42A and 42B. In detail, the first flow rate F1 is greater than five times the second flow rate F2 (the first flow rate F1 is an order greater than the second flow rate F2). When the standby (preparation process) is completed, the control unit 45 causes the display unit 70 to pop up the "Standby Completed" and "Confirm" buttons. When the "Confirm" button is pressed and held (operated) by the user (the display unit 70 accepts an operation confirming that the standby is completed), the control unit 45 determines that the user has confirmed that the standby is completed. The control unit 45 executes the culture mode on the condition that it is determined that the user has confirmed that the standby is completed.

The control unit 45 selectively executes a pressure control mode or a flow control mode as a culture mode. The display unit 70 displays a "pressure control mode" button and a "flow control mode" button. The user selects the pressure control mode or the flow control mode by pressing (operating) the "pressure control mode" button or the "flow control mode" button in advance.

In the pressure control mode, the user operates the image displayed in advance by the display unit 70 to input a set pressure P2s, which is a target value of the pressure P2 detected by the pressure sensor 84. The set pressure P2s is a pressure lower than the above-mentioned damage pressure Pd and is a pressure suitable for culturing the cell tissue B. In the pressure control mode (cultivation mode, second mode), the control unit 45 controls the pressure control liquid delivery unit 20 to set the pressure P2 detected by the pressure sensor 84 to the set pressure P2s.

In the flow control mode, the user operates the image displayed in advance on the display unit 70 to input a set flow rate Fs, which is a target value of the flow rate F detected by the flow sensor 85. The set flow rate Fs is a flow rate suitable for culturing cell tissue B. In the flow control mode (cultivation mode, second mode), the control unit 45 controls the pressure-controlled liquid delivery unit 20 to set the flow rate F detected by the flow sensor 85 to the set flow rate Fs.

Figure 2 is a flowchart showing the steps of the standby mode. This series of processes is executed by the control unit 45 when the user performs an operation to start the standby mode on the display unit 70.

First, it is determined whether the liquid level sensor 41 is ON and whether the pressure P1 is less than the threshold pressure P1r (S10). That is, it is determined whether the liquid level detected by the liquid level sensor 41 is higher than a predetermined position and whether the pressure P1 of the culture fluid L at the bottom of the tank 34 detected by the pressure sensor 37 (hereinafter referred to as "pressure P1 inside the tank 34") is lower than the threshold pressure P1r. The threshold pressure P1r is a pressure that is lower than the above-mentioned damage pressure Pd and does not prevent the culture fluid L from being rapidly sent to the cell culture tank 50. In this determination, if it is determined that the liquid level sensor 41 is not ON or that the pressure P1 is not less than the threshold pressure P1r (S10: NO), the determination of S10 is executed again.

On the other hand, if it is determined that the liquid level sensor 41 is ON and the pressure P1 is less than the threshold pressure P1r (S10: YES), the solenoid valve 46 is opened to allow the culture liquid L to be sent from the tank 34 to the cell culture tank 50 (S11).

Next, it is determined whether pressure P2<threshold pressure P2r (S12). That is, it is determined whether pressure P2 inside cell tissue B detected by pressure sensor 84 is lower than threshold pressure P2r. Threshold pressure P2r is a pressure that is lower than damage pressure Pd and does not prevent culture fluid L from being rapidly sent to cell culture tank 50. Note that threshold pressure P1r and threshold pressure P2r may be the same or different. In this determination, if it is determined that pressure P2 is not lower than threshold pressure P2r (S12: NO), pressure control fluid sending unit 20 is controlled to reduce the pressure inside tank 34 (S13). Then, the determination in S12 is executed again.

On the other hand, if it is determined that pressure P2<threshold pressure P2r (S12: YES), it is determined whether pressure P1≈set pressure P1s (S14). That is, it is determined whether pressure P1 inside tank 34 detected by pressure sensor 37 is approximately equal to the set pressure P1s. If pressure P1≈set pressure P1s, culture fluid L is supplied to cell culture tank 50 at the first flow rate F1 through fluid supply pipes 42A, 42B. If it is determined that pressure P1≈set pressure P1s is not the case (S14: NO), pressure control fluid supply unit 20 is controlled so that pressure inside tank 34 approaches set pressure P1s (S15). Then, the process is executed again from the determination in S12.

On the other hand, if it is determined in step S14 that pressure P1 is approximately equal to the set pressure P1s (S14: YES), the pressure control liquid delivery unit 20 is controlled to maintain the pressure inside the tank 34 at the set pressure P1s (S16).

Next, it is determined whether or not the dissolved oxygen concentration DO is approximately equal to the target dissolved oxygen concentration, pH is approximately equal to the target pH, and temperature T is approximately equal to the target temperature (S17). Note that in a culture solution conditioning control separate from the flowchart in FIG. 2, the control unit 45 controls the MFCs 61-63 and the heater 52 so that the dissolved oxygen concentration DO is approximately equal to the target dissolved oxygen concentration, pH is approximately equal to the target pH, and temperature T is approximately equal to the target temperature. In this determination, if it is determined that any of the pH, dissolved oxygen concentration DO, and temperature T is not approximately equal to the target value (S17: NO), the process is repeated from S12.

On the other hand, if it is determined that the dissolved oxygen concentration DO is ≈ the target dissolved oxygen concentration, the pH is ≈ the target pH, and the temperature T is ≈ the target temperature (S17: YES), the display unit 70 pops up a "Standby Completed" and "Confirm" button (S18). That is, in standby mode, the MFCs 61-63 and the heater 52 are controlled to bring the state of the culture solution L detected by the sensors 81-83 to the target state, and an image indicating that standby has been completed is displayed on the display unit 70, provided that the state of the culture solution L detected by the sensors 81-83 has reached the target state.

Then, it is determined whether the user has confirmed that the standby state has been completed (S19). In this determination, if it is determined that the user has not confirmed that the standby state has been completed (S19: NO), the process is executed again from S12. That is, after an image indicating that the standby state has been completed is displayed on the display unit 70, the MFCs 61-63 and the heater 52 are controlled to maintain the state of the culture solution L detected by the sensors 81-83 at the target state until the display unit 70 receives an operation to confirm that the standby state has been completed.

On the other hand, if it is determined in the judgment of S19 that the user has confirmed that the standby has been completed (S19: YES), the solenoid valve 46 is closed, the supply of oxygen, carbon dioxide, and nitrogen to the mixer 60 is stopped, and the pressure control inside the tank 34 by the pressure control liquid delivery unit 20 is stopped (S20). That is, when the operation confirming that the standby has been completed is received by the display unit 70, the supply of culture liquid L to the cell culture tank 50 through the liquid delivery pipes 42A and 42B and the supply of gas to the cell culture tank 50 through the gas pipe 65 are stopped. After that, this series of processes is ended (END), and the user puts the cell tissue B into the cell culture tank 50 to make it ready for culture. In detail, the cell storage unit 51 containing the cell tissue B is installed inside the cell culture tank 50, and the liquid delivery pipe 42B is connected to the cell storage unit 51.

Figure 3 is a flowchart showing the steps of the culture mode. This series of processes is executed when the user operates the display unit 70 to start the culture mode.

First, the pump 90 executes tank liquid level control (S30). In detail, the pump 90 pumps the culture liquid L when the liquid level detected by the liquid level sensor 41 falls below a predetermined position (liquid level sensor 41: OFF). Then, the pump 90 stops pumping the culture liquid L when the liquid level detected by the liquid level sensor 41 rises above the predetermined position (liquid level sensor 41: ON).

Next, the control unit 45 executes culture solution conditioning control (S31). In detail, the control unit 45 controls the MFCs 61 to 63 and the heater 52 so that the dissolved oxygen concentration DO is approximately equal to the target dissolved oxygen concentration, the pH is approximately equal to the target pH, and the temperature T is approximately equal to the target temperature.

Next, the control unit 45 executes the liquid delivery control (S32). Figure 4 is a flowchart showing the procedure for the liquid delivery control.

As shown in the figure, it is determined whether pressure P2<threshold pressure P2r (S321). In other words, it is determined whether the pressure P2 inside the cell tissue B detected by the pressure sensor 84 is lower than the threshold pressure P2r. In this determination, if it is determined that pressure P2<threshold pressure P2r is not satisfied (S321: NO), the pressure control liquid delivery unit 20 is controlled to lower the pressure inside the tank 34 (S322). After that, the process returns to the next process of S32 in FIG. 3 (RET).

On the other hand, if it is determined that the pressure P2 is less than the threshold pressure P2r (S321: YES), it is determined whether the pressure P2 is approximately equal to the set pressure P2s or whether the flow rate F is approximately equal to the set flow rate Fs (S323). That is, if the pressure control mode is being executed, it is determined whether the pressure P2 inside the cell tissue B detected by the pressure sensor 84 is approximately equal to the set pressure P2s. If it is determined that the pressure P2 is not approximately equal to the set pressure P2s (S323: NO), the pressure control liquid delivery unit 20 is controlled so as to bring the pressure P2 closer to the set pressure P2s (S324). Also, if the flow control mode is being executed, it is determined whether the flow rate of the culture liquid L detected by the flow sensor 85 is approximately equal to the set flow rate Fs. If it is determined that the flow rate F is not approximately equal to the set flow rate Fs (S323: NO), the pressure control liquid delivery unit 20 is controlled so as to bring the flow rate F closer to the set flow rate Fs (S324). Then, return to the next process at S32 in Figure 3 (RET).

On the other hand, if it is determined in S323 that the pressure P2 is approximately equal to the set pressure P2s or the flow rate F is approximately equal to the set flow rate Fs (S323: YES), the pressure control liquid delivery unit 20 is controlled to maintain the pressure inside the tank 34 at the current pressure (S325). After that, the process returns to the next process of S32 in FIG. 3 (RET).

Returning to FIG. 3, in the process of S33, the control unit 45 determines whether or not a culture stop operation has been performed by the user (S33). More specifically, it determines whether or not a culture mode stop operation has been performed by the user on the display unit 70. If it is determined in this determination that the user has not performed the culture stop operation (S33: NO), the process is executed again from S30. On the other hand, if it is determined in this determination that the user has performed the culture stop operation (S33: YES), this series of processes is terminated (END).

The present embodiment described above has the following advantages:

The control unit 45 executes a standby mode in which it controls the pressure-controlled liquid delivery unit 20 to supply the culture liquid L to the cell culture tank 50 at a first flow rate F1 via the liquid delivery pipes 42A, 42B. Therefore, before the cell tissue B is stored in the cell culture tank 50, i.e., when there is no risk of damaging the cell tissue B, the standby mode can be executed, and the culture liquid L can be supplied to the cell culture tank 50 at the first flow rate F1 via the liquid delivery pipes 42A, 42B. Therefore, the time required for the preparation process before starting cell culture (for example, the process of storing the culture liquid L with an adjusted dissolved gas concentration in the cell culture tank 50) can be shortened.

Then, the control unit 45 executes a culture mode in which the pressure P2 detected by the pressure sensor 84 after the standby mode ends is made lower than the predetermined damage pressure Pd at which the predetermined cell tissue B begins to be damaged, and the pressure control liquid supply unit 20 is controlled so as to supply the culture liquid L to the cell culture tank 50 through the liquid supply pipes 42A and 42B at a second flow rate F2 smaller than the first flow rate F1. Therefore, the culture mode is executed after the cell tissue B is stored in the cell culture tank 50, and the pressure P2 of the culture liquid L supplied to the cell culture tank 50 through the liquid supply pipes 42A and 42B is detected by the pressure sensor 84, and the pressure P2 of the culture liquid L is made lower than the damage pressure Pd and the flow rate F of the culture liquid L is set to the second flow rate F2 smaller than the first flow rate F1, thereby preventing the predetermined cell tissue B from being damaged by the flow of the culture liquid L. Moreover, by executing the culture mode after the standby mode, the preparation process (standby) and the cell culture process before starting cell culture can be easily executed, and the user's effort in executing the cell tissue B culture can be reduced.

- The cell storage section 51 stores the cell tissue B. The cell storage section 51 is stored inside the cell culture tank 50. Therefore, by placing the cell storage section 51 storing the specified cell tissue B in the cell culture tank 50, the cell tissue B can be easily placed in the cell culture tank 50.

- The liquid supply pipe 42B is inserted from the outside to the inside of the cell culture tank 50 and connected to the cell storage section 51. This allows the culture fluid L to be directly supplied to the inside of the cell storage section 51, making it easier to create a flow of culture fluid L inside the cell tissue B stored in the cell storage section 51. This makes it easier to culture the cell tissue B in an appropriate environment where a flow of culture fluid L is created inside the cell tissue B, and the culture of the cell tissue B can be promoted. Furthermore, since the pressure P2 detected by the pressure sensor 84 is set lower than a predetermined damage pressure Pd at which a predetermined cell tissue B begins to be damaged, damage to the cell tissue B due to the flow of culture fluid L can be suppressed.

The pressure sensor 84 detects the pressure P2 of the culture fluid L supplied to the cell culture tank 50 through the liquid supply pipe 42B inside the cellular tissue B. With this configuration, the pressure sensor 84 can detect the pressure P2 of the culture fluid L inside the cellular tissue B, which further prevents the cellular tissue B from being damaged by the flow of the culture fluid L. In addition, the pressure P2 of the culture fluid L inside the cellular tissue B, which serves as the environment in which the cellular tissue B is cultured, can be more accurately grasped.

The pressure receiving unit (display unit 70) receives the input of the set pressure P2s. Therefore, the pressure of the culture fluid L in the cell culture process can be set arbitrarily. However, the set pressure P2s must be set lower than the damage pressure Pd and the flow rate F of the culture fluid L must be set to a second flow rate F2 that is lower than the first flow rate F1. The control unit 45 then executes a pressure control mode as the culture mode, in which the pressure control fluid delivery unit 20 is controlled so that the pressure P2 detected by the pressure sensor 84 becomes the set pressure P2s received by the pressure receiving unit. Therefore, the pressure control mode is executed as the culture mode, and cell culture can be performed in a state in which the pressure P2 detected by the pressure sensor 84 is maintained at the set pressure P2s.

- The flow rate receiving unit (display unit 70) receives input of the set flow rate Fs. Therefore, the flow rate F of the culture fluid L in the cell culture process can be set arbitrarily. However, the set flow rate Fs needs to be set smaller than the first flow rate F1. Then, the control unit 45 executes a flow rate control mode as the culture mode, in which the pressure-controlled liquid delivery unit 20 is controlled so that the flow rate F detected by the flow rate sensor 85 becomes the set flow rate Fs received by the flow rate receiving unit. Therefore, the flow rate control mode is executed as the culture mode, and cell culture can be performed in a state in which the flow rate F detected by the flow rate sensor 85 is maintained at the set flow rate Fs.

The control unit 45 can selectively execute a pressure control mode or a flow control mode. Therefore, depending on the purpose and the type of cell tissue B, a mode suitable for culturing can be selected and executed.

- The first flow rate F1 is greater than five times the second flow rate F2. Therefore, the first flow rate F1 of the culture solution L in the standby mode can be greater than five times the second flow rate F2 of the culture solution L in the culture mode, and the time required for the preparation process can be significantly reduced.

- The standby mode is basically executed before the cell tissue B is stored in the cell culture tank 50, that is, in a state where there is no risk of damaging the cell tissue B. However, if the pressure of the culture fluid L in the standby mode is higher than the damage pressure Pd, there is a risk that the culture fluid L at a pressure higher than the damage pressure Pd will temporarily flow into the cell tissue B when switching to the culture mode. In this regard, the control unit 45 makes the pressure P1 detected by the pressure sensor 37 lower than the damage pressure Pd (threshold pressure P1r) in the standby mode. Therefore, it is possible to prevent the culture fluid L at a pressure higher than the damage pressure Pd from temporarily flowing into the cell tissue B when switching from the standby mode to the culture mode.

Sensors 81-83 detect the state of the culture solution L. The state of the culture solution L is determined by the pH, dissolved oxygen concentration DO, and temperature T of the culture solution L. MFCs 61-63 and heater 52 adjust the state of the culture solution L. Specifically, the pH can be adjusted by dissolving carbon dioxide in the culture solution L, the dissolved oxygen concentration DO can be adjusted by dissolving oxygen in the culture solution L, and the temperature T can be adjusted by heating the culture solution L with heater 52.

- In standby mode, the control unit 45 controls the MFCs 61-63 and the heater 52 to make the state detected by the sensors 81-83 the target state. Therefore, by executing the standby mode, the actual state of the culture solution L can be made the target state. Then, the control unit 45 causes the display unit 70 to display an image indicating that the preparation process is complete, on the condition that the state detected by the sensors 81-83 has become the target state. Therefore, the user can easily know that the preparation process is complete by confirming that the image indicating that the preparation process is complete is displayed by the display unit 70. In addition, the user can easily know that the preparation process is not complete by confirming that the image indicating that the preparation process is complete is not displayed by the display unit 70.

- The confirmation receiving unit (display unit 70) receives an operation that confirms that the preparation process has been completed. Then, when the control unit 45 receives the operation from the confirmation receiving unit, it stops the supply of culture fluid L to the cell culture tank 50 through the liquid delivery pipes 42A, 42B. Therefore, when the user performs an operation that confirms that the preparation process has been completed, the supply of culture fluid L to the cell culture tank 50 can be stopped, and the process of storing the cell tissue B in the cell culture tank 50 can be safely proceeded to.

- After displaying an image indicating that the preparation process is complete on the display unit 70, the control unit 45 controls the MFCs 61-63 and the heater 52 to maintain the state detected by the sensors 81-83 in the target state until an operation is received by the confirmation receiving unit. With this configuration, the state of the culture solution L can be maintained in the target state after the preparation process is completed, until the user performs an operation that confirms that the preparation process is complete. Therefore, the user can perform an operation that confirms that the preparation process is complete at a time desired by the user to start cell culture. Furthermore, since the state detected by the sensors 81-83 is maintained in the target state until an operation is received by the confirmation receiving unit, the conditions for starting cell culture can be aligned, and the reproducibility of cell culture can be improved.

- While the standby mode is in progress, the pH, dissolved oxygen concentration DO, and temperature T of the culture solution L can be set to the target pH, target dissolved oxygen concentration, and target temperature, respectively. Therefore, the cell culture process can be quickly started after the standby mode is completed, reducing the user's effort.

The above embodiment can be modified as follows. The same parts as those in the above embodiment are designated by the same reference numerals and will not be described.

- In the processes of S13 and S15 in FIG. 2 and S322 and S324 in FIG. 4, the range by which the pressure inside the tank 34 is changed and the period by which the pressure is changed may be variably set. Also, the range of pressures over which pressure P1 is considered to be ≒ P1s and the range of pressures over which pressure P2 is considered to be ≒ P2s may be variably set.

- The control unit 45 may stop the pressure-controlled liquid delivery unit 20 when the flow rate F detected by the flow rate sensor 85 is an abnormal flow rate. With this configuration, the flow of the culture liquid L can be stopped when the culture liquid L cannot be circulated normally. Therefore, the burden on the user to monitor the cell culture system 10 during the standby mode and the culture mode can be reduced. Furthermore, the control unit 45 may notify the user that the flow of the culture liquid L is abnormal by the display unit 70 or a warning light when the flow rate F detected by the flow rate sensor 85 is an abnormal flow rate.

- The control unit 45 may stop the MFCs 61-63 and the heater 52 when the state detected by the sensors 81-83 is abnormal. With this configuration, it is possible to stop the adjustment of the state of the culture solution L when the state of the culture solution L cannot be adjusted normally. This reduces the burden on the user to monitor the cell culture system 10 during the standby mode and culture mode. Furthermore, the control unit 45 may notify the user that the state of the culture solution L is abnormal by the display unit 70 or a warning light when the state detected by the sensors 81-83 is abnormal.

After the control unit 45 causes the display unit 70 to display an image indicating that the preparation process has been completed, the control unit 45 can also omit the control of maintaining the state detected by the sensors 81 to 83 at the target state until an operation is received by the confirmation receiving unit.

- When the confirmation receiving unit receives an operation confirming that the preparation process has been completed, the control unit 45 can omit the process of stopping the supply of culture fluid L to the cell culture tank 50 through the liquid supply pipes 42A and 42B. In addition, the supply of culture fluid L to the cell culture tank 50 through the liquid supply pipes 42A and 42B may be stopped manually.

-The target state of the culture medium L can also be set in advance by program.

- Adjustment of at least one of the pH, dissolved oxygen concentration DO, and temperature T of the culture liquid L can be omitted or can be performed manually. Accordingly, detection of at least one of the pH, dissolved oxygen concentration DO, and temperature T of the culture liquid L can be omitted or can be performed manually.

- In standby mode, the control unit 45 does not necessarily have to make the pressure P1 detected by the pressure sensor 37 lower than the damage pressure Pd.

- The first flow rate F1 is not limited to being set to a value greater than five times the second flow rate F2, but may be set to a value greater than twice the second flow rate F2, or may be set to a value greater than one time the second flow rate F2.

- The set pressures P1s, P2s and set flow rate Fs of the culture solution L can also be set in advance by programming.

- The control unit 45 may be configured to execute only one of the pressure control mode and the flow rate control mode.

- A pressure sensor 84 that detects the pressure inside the cell tissue B (flow path Bp) can also be inserted into the cell tissue B from the liquid supply pipe 42B. In addition, as the pressure P2 of the culture liquid L supplied to the cell culture tank 50 by the liquid supply pipes 42A and 42B, the pressure P2 of the culture liquid L just before the cell culture tank 50 in the liquid supply pipe 42B can be detected by a pressure sensor 86, as shown by the dashed line in FIG. 1.

-A variable-capacity precision pump can be used instead of the pressure-controlled liquid delivery unit 20.

The cell culture system 10 may be equipped with a storage device, and various parameters may be stored in the storage device at any time. The cell culture system 10 may also be configured to allow an external storage medium to be inserted, and various parameters may be stored in the external storage medium at any time. With these configurations, evidence of testing can be easily preserved. The time interval for storing various parameters may also be variably set.

- The cell culture system 10 may be capable of communicating with an external device, allowing the external device to acquire various data and execute various settings. The cell culture system 10 may also be capable of storing various data in the cloud. Furthermore, the communication function may enable users in remote locations to share data in real time or hold real time meetings. Furthermore, when it is difficult for a user to operate the display unit 70, similar operations may be performed using a tablet terminal or the like capable of communicating with the cell culture system 10.

10...cell culture system, 20...pressure controlled liquid delivery section (flow rate adjustment section), 34...tank, 37...pressure sensor, 42A...liquid delivery pipe (supply pipe), 42B...liquid delivery pipe (supply pipe), 45...control section, 50...cell culture tank (culture tank), 51...cell storage section, 70...display section (pressure reception section, flow rate reception section, confirmation reception section), 81...oxygen concentration sensor (status sensor), 82...pH sensor (status sensor), 83...temperature sensor (status sensor), 84...pressure sensor, 85...flow rate sensor, 86...pressure sensor.

Claims (14)

A culture tank for storing a culture solution;
A supply pipe for supplying the culture solution from the outside to the inside of the culture tank;
A flow rate adjusting unit that adjusts the flow rate of the culture solution flowing through the supply pipe;
A pressure sensor for detecting a pressure of the culture solution supplied to the culture tank through the supply pipe;
A control unit that executes a first mode in which the flow rate adjustment unit is controlled to supply the culture solution to the culture tank at a first flow rate through the supply pipe before a predetermined cell tissue is stored in the culture tank , and a second mode in which the flow rate adjustment unit is controlled to lower the pressure detected by the pressure sensor after the first mode is completed below a predetermined damage pressure at which the cell tissue begins to be damaged and to supply the culture solution to the culture tank at a second flow rate lower than the first flow rate through the supply pipe;
A cell culture system comprising: A cell storage section for storing the cell tissue,
The cell storage unit is stored inside the culture tank,
The cell culture system according to claim 1 , wherein the supply pipe is inserted from the outside to the inside of the culture tank and connected to the cell storage unit. The cell culture system according to claim 2, wherein the pressure sensor detects the pressure of the culture medium supplied to the culture tank through the supply pipe inside the cell tissue. A pressure receiving unit is provided for receiving an input of a set pressure,
The cell culture system according to any one of claims 1 to 3, wherein the control unit executes a pressure control mode as the second mode in which the control unit controls the flow rate control unit so that the pressure detected by the pressure sensor becomes the set pressure received by the pressure receiving unit. A flow rate receiving unit that receives an input of a set flow rate;
A flow rate sensor for detecting a flow rate of the culture solution supplied to the culture tank through the supply piping;
The control unit executes a flow control mode as the second mode in which the flow rate detected by the flow sensor is controlled to the set flow rate received by the flow rate receiving unit. The cell culture system according to any one of claims 1 to 4. A pressure receiving unit that receives an input of a set pressure;
A flow rate receiving unit that receives an input of a set flow rate;
A flow rate sensor for detecting a flow rate of the culture solution supplied to the culture tank through the supply piping;
The control unit selectively executes, as the second mode, a pressure control mode in which the flow rate adjustment unit is controlled so that the pressure detected by the pressure sensor becomes the set pressure received by the pressure receiving unit, and a flow rate control mode in which the flow rate adjustment unit is controlled so that the flow rate detected by the flow sensor becomes the set flow rate received by the flow rate receiving unit.The cell culture system described in any one of claims 1 to 3. The cell culture system according to any one of claims 1 to 6, wherein the first flow rate is greater than five times the second flow rate. The cell culture system according to any one of claims 1 to 7, wherein the control unit, in the first mode, makes the pressure detected by the pressure sensor lower than the damage pressure. A state sensor for detecting a state of the culture solution;
A condition adjusting unit that adjusts the condition of the culture solution;
A display unit that displays an image,
The cell culture system according to any one of claims 1 to 8, wherein in the first mode, the control unit controls the condition adjustment unit to make the condition detected by the condition sensor a target state, and causes the display unit to display an image indicating that the preparation process has been completed, on condition that the condition detected by the condition sensor has become the target state. a confirmation receiving unit that receives an operation that confirms that the preparation process has been completed,
The cell culture system according to claim 9 , wherein the control unit stops the supply of the culture medium to the culture tank through the supply pipe when the operation is accepted by the confirmation accepting unit. The cell culture system according to claim 10, wherein the control unit controls the condition adjustment unit to maintain the state detected by the condition sensor at a target state after causing the display unit to display an image indicating that the preparation process has been completed, until the confirmation reception unit receives the operation. The cell culture system according to any one of claims 9 to 11, wherein the state of the culture medium includes the pH, dissolved oxygen concentration, and temperature of the culture medium. The cell culture system according to claim 5 or 6, wherein the control unit stops the flow rate adjustment unit when the flow rate detected by the flow rate sensor is an abnormal flow rate. The cell culture system according to any one of claims 9 to 12, wherein the control unit stops the condition adjustment unit when the condition detected by the condition sensor is an abnormal condition.

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