JP7699074B2 - Fertilizer component absorption promoter for plants and fertilizer for plants containing the same - Google Patents

Description

The present invention relates to a fertilizer component absorption enhancer for plants and a plant fertilizer containing the same.

Technologies have been developed to adjust plant growth, with the aim of improving the supply efficiency of grain plants and horticultural plants. In addition to measures such as optimizing temperature and sunlight conditions and fertilization, methods have been reported to improve the absorption efficiency of applied fertilizer components.

Patent Document 1 discloses a plant activator that uses fatty acids or their derivatives to improve the absorption of at least one of the main components of plant fertilizer, nitrate ions, phosphate ions, and potassium ions, by 10% or more.

However, the plant activator described in Patent Document 1 still does not sufficiently improve the absorption efficiency of fertilizer components.

JP 2001-288011 A

The purpose of the present invention is to improve the absorption efficiency of fertilizer components (potassium, phosphorus, nitrogen) in the plant body to which the fertilizer is applied.

The present invention relates to a fertilizer component absorption promoter for plants, which contains an unsaturated oxo fatty acid or a derivative or salt thereof.

The plant fertilizer component absorption promoter preferably further contains a hydroxylated fatty acid or a derivative or a salt thereof.

It is preferable that the plant fertilizer component absorption promoter further contains an unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid).

The plant fertilizer component absorption promoter, wherein the unsaturated oxo fatty acid or a derivative or a salt thereof has the following formula (I):
HOOC-(R ¹ )-CH=CH-C(=O)-R ² (I)
(In the formula,
R ¹ : a linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms;
^R2 : an alkyl group having 2 to 8 carbon atoms, which may contain one or more branches and/or double bonds.
or a derivative or a salt thereof.

In the plant fertilizer component absorption promoter, the unsaturated oxo fatty acid is an unsaturated oxo fatty acid in which the hydrocarbon group of R ¹ has 8 to 10 carbon atoms and the alkyl group of R ² has 4 to 6 carbon atoms.

In the fertilizer component absorption promoter for plants, the unsaturated oxo fatty acid is an unsaturated oxo fatty acid in which R ¹ of the unsaturated oxo fatty acid contains a double bond that forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I).

The plant fertilizer component absorption promoter is an unsaturated oxo fatty acid in which R ¹ is a linear or branched hydrocarbon group having 9 carbon atoms and R ² is an alkyl group having 5 carbon atoms.

The plant fertilizer component absorption promoter, wherein the unsaturated oxo fatty acid is ketooctadecadienoic acid.

The plant fertilizer component absorption promoter, wherein the unsaturated oxo fatty acid is 13-oxo-9,11-octadecadienoic acid.

The plant fertilizer component absorption promoter, wherein the hydroxylated fatty acid or its derivative or salt thereof is represented by the following formula (II) and/or (III):
HOOC-(R ³ )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R ⁴ (II)
HOOC-(R ³ )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R ⁴ (III)
(In the formula,
^R3 is a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and/or OH groups, and when it contains a double bond, the position of the double bond is not limited;
^R4 is a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and/or OH groups, and when it contains a double bond, the position of the double bond is not limited.
or a derivative or a salt thereof.

In the fertilizer component absorption promoter for plants, the hydroxylated fatty acid has a hydrocarbon group of ^R3 having 6 to 8 carbon atoms and a hydrocarbon group of ^R4 having 4 to 6 carbon atoms.

In the plant fertilizer component absorption promoter, the hydroxylated fatty acid has a structure in which R ³ has a -(CH ₂ ) _n - (n is an integer from 4 to 12) structure and R ⁴ has a structure of C _n H _2n+1 - (n is an integer from 2 to 8).

In the fertilizer component absorption promoter for plants, the hydroxylated fatty acid has R ³ which is an alkylene group having 7 carbon atoms (-(CH ₂ ) ₇ --) and R ⁴ which is an alkyl group having 5 carbon atoms (CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ --).

The plant fertilizer component absorption promoter, wherein the hydroxylated fatty acid is hydroxyoctadecenoic acid.

The fertilizer component absorption promoter for plants, wherein the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecenoic acid.

The fertilizer component absorption promoter for plants, wherein the hydroxylated fatty acid is 9,12,13-trihydroxy-10-octadecenoic acid.

The fertilizer component absorption promoter for plants, wherein the unsaturated fatty acid (excluding unsaturated oxo fatty acids and unsaturated hydroxylated fatty acids) is an unsaturated fatty acid having 10 to 20 carbon atoms.

The fertilizer component absorption promoter for plants, wherein the unsaturated fatty acid (excluding unsaturated oxo fatty acids and unsaturated hydroxylated fatty acids) is a mono- or polyunsaturated fatty acid containing at least one selected from the group consisting of oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid.

The plant fertilizer component absorption promoter, wherein the unsaturated fatty acid is linoleic acid.

A plant fertilizer containing the plant fertilizer component absorption promoter and plant fertilizer components.

Note that "octadecenoic acid" is also commonly written as "octadecaenoic acid" (for example, JP-A-3-14539, etc.), and the above-mentioned "9,10,13-trihydroxy-11-octadecenoic acid" is also written as "9,10,13-trihydroxy-11-octadecenoic acid" or "9,10,13-trihydroxyoctadec-11-enoic acid". Similarly, the above-mentioned "9,12,13-trihydroxy-10-octadecenoic acid" is also written as "9,12,13-trihydroxy-10-octadecenoic acid" or "9,12,13-trihydroxyoctadec-10-enoic acid". Note that in the examples, the manufacturer's name is also written in parentheses. The above explanation applies to all "octadecenoic acid" used in this specification, claims, drawings, and abstract.

The structural formula of "9,10,13-trihydroxy-11-octadecenoic acid" is shown in the following structural formula (1).

The structural formula of "9,12,13-trihydroxy-10-octadecenoic acid" is shown in the following structural formula (2).

As the derivatives of the unsaturated oxo fatty acids and hydroxylated fatty acids, esters of the unsaturated oxo fatty acids and hydroxylated fatty acids are preferred. As salts of the unsaturated oxo fatty acids and hydroxylated fatty acids, salts such as sodium salts, potassium salts, and ammonium salts, which will be described later, can be used.

The plant fertilizer component absorption promoter is preferably used as a spray or immersion agent that is brought into contact with the roots of a plant, or as a soil drench agent, or mixed with a plant fertilizer or plant fertilizer components.

It is preferable that the plant fertilizer component absorption promoter is used for plants selected from the Brassicaceae, Poaceae, Leguminosae, Solanaceae, Rosaceae, Amaranthaceae, and Malvaceae families.

The plant fertilizer component absorption promoter of the present invention may be in liquid or powder form. When in powder form, an aqueous dispersion in which an unsaturated oxo fatty acid or a derivative or a salt thereof (which may contain a hydroxylated fatty acid or a derivative or a salt thereof and/or an unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid) as necessary) is dispersed is freeze-dried to obtain a powder form.

It is also preferable that the aqueous dispersion contains a stabilizer.

It is also preferable that the stabilizer is an emulsifier.

Furthermore, it is desirable that the emulsifier is at least one selected from potassium carbonate and dipotassium hydrogen phosphate.

The plant fertilizer component absorption promoter of the present invention can improve the absorption efficiency of the fertilizer components potassium, phosphorus, and nitrogen in plants, and promote plant growth. In addition, the plant fertilizer containing the plant fertilizer component absorption promoter of the present invention and the plant fertilizer components has a high absorption efficiency of the fertilizer components, and therefore has a high effect of promoting plant growth.

The plant fertilizer component absorption promoter of the present invention contains an unsaturated oxo fatty acid or a derivative or salt thereof.

The fertilizer component absorption promoter of the present invention contains an unsaturated oxo fatty acid or a derivative or salt thereof. By applying this fertilizer component absorption promoter to a part of the roots of a plant, adding it to the soil, or mixing it with a plant fertilizer, the absorption efficiency of potassium, phosphorus, and nitrogen in the soil or plant fertilizer can be increased, and plant growth can be promoted.

The plant fertilizer component absorption promoter of the present invention preferably further contains a hydroxylated fatty acid or a derivative or a salt thereof. Hydroxylated fatty acids or derivatives or salts thereof are recognized to have a plant growth promoting effect, and can increase the yield of plants such as vegetables, grains, and fruits.

In addition, in the case of fertilizer component absorption promoters for plants that contain unsaturated fatty acids (excluding unsaturated oxo fatty acids and unsaturated hydroxylated fatty acids) as active ingredients, the soil, fertilizer, and active ingredients may be washed away from the site of action by rain, etc. Such washing away of the active ingredients by rain, wind erosion, and other erosive forces may significantly reduce the duration of the fertilizer component absorption promoter for plants.

The plant fertilizer component absorption promoter of the present invention further contains an unsaturated fatty acid in addition to the above-mentioned active ingredients, i.e., an unsaturated oxo fatty acid or a derivative or a salt thereof, and a hydroxylated fatty acid or a derivative or a salt thereof. The unsaturated fatty acid of the present invention does not include an unsaturated oxo fatty acid or an unsaturated hydroxylated fatty acid. The unsaturated fatty acid contained in the plant fertilizer component absorption promoter of the present invention is hydrophobic and has low water solubility. As a result, the unsaturated fatty acid of the present invention can impart water-repellent properties to the plant fertilizer component absorption promoter in the soil, in the fertilizer, and at the inoculation site. In other words, the plant fertilizer component absorption promoter of the present invention is hardly washed off. Therefore, the fertilizer component absorption promotion effect lasts for a long period of time.

The unsaturated oxo fatty acid or a derivative or a salt thereof of the present invention is represented by the following formula (I):
HOOC-(R ¹ )-CH=CH-C(=O)-R ² (I)
(In the formula,
R ¹ : a linear or branched, saturated or unsaturated hydrocarbon group containing 6 to 12 carbon atoms; R ² : an alkyl group containing 2 to 8 carbon atoms, which may contain one or more branches and/or double bonds.
An unsaturated oxo fatty acid having the structural formula: or a derivative or salt thereof can be suitably used.

In one embodiment of the present invention, the hydrocarbon group of R ¹ in the unsaturated oxo fatty acid has 8 to 10 carbon atoms, and the alkyl group of R ² has 4 to 6 carbon atoms. In another embodiment, R ¹ in the unsaturated oxo fatty acid contains a double bond that forms a conjugated double bond with the double bond between the α and β carbons of the carbonyl group in formula (I). In yet another embodiment, it is preferred that R ¹ in the unsaturated oxo fatty acid is a linear or branched hydrocarbon group having 9 carbon atoms, and R ² is an alkyl group having 5 carbon atoms.

Specific examples of the unsaturated oxo fatty acids used in the present invention include ketooctadecadienoic acid. For example, ketooctadecadienoic acid includes, but is not limited to, 9-oxo-10,12-octadecadienoic acid (9-oxoODA), 13-oxo-9,11-octadecadienoic acid (13-oxoODA), 5-oxo-6,8-octadecadienoic acid, 6-oxo-9,12-octadecadienoic acid, 8-oxo-9,12-octadecadienoic acid, 10-oxo-8,12-octadecadienoic acid, 11-oxo-9,12-octadecadienoic acid, 12-oxo-9,13-octadecadienoic acid, and 14-oxo-9,12-octadecadienoic acid, as well as isomers thereof.

As the derivative of the unsaturated oxo fatty acid, an ester is preferable. Examples of the ester of the unsaturated oxo fatty acid of the present invention include, but are not limited to, methyl ester, ethyl ester, propyl ester, butyl ester, pentyl ester, isopentyl ester, octyl ester, etc. In addition, examples of the salt of the unsaturated oxo fatty acid include ammonium salts such as alkyl ammonium salts such as ammonium salts and tetramethylammonium salts, alkaline earth metal salts such as calcium salts and magnesium salts, alkali metal salts such as sodium salts, lithium salts, potassium salts, and metal salts such as cobalt salts and manganese salts, but are not particularly limited as long as they are one or more agriculturally acceptable salts such as salts contained in fertilizers, etc.

The hydroxylated fatty acid or its derivative or salt thereof used in the present invention is represented by the following formula (II) and/or (III):
HOOC-(R ³ )-CH(OH)-CH(OH)-CH=CH-CH(OH)-R ⁴ (II)
HOOC-(R ³ )-CH(OH)-CH=CH-CH(OH)-CH(OH)-R ⁴ (III)
(In the formula,
^R3 is a linear or branched hydrocarbon group having 4 to 12 carbon atoms, which may contain one or more double bonds and/or OH groups, and when it contains a double bond, the position of the double bond is not limited;
^R4 is a linear or branched hydrocarbon group having 2 to 8 carbon atoms, which may contain one or more double bonds and/or OH groups, and when it contains a double bond, the position of the double bond is not limited.
A hydroxylated fatty acid having the structural formula: or a derivative or salt thereof can be suitably used.

In one embodiment of the present invention, the hydrocarbon group of R ³ in the hydroxylated fatty acid has 6 to 8 carbon atoms, and the hydrocarbon group of R ⁴ has 4 to 6 carbon atoms. In another embodiment, R ³ in the hydroxylated fatty acid has a structure of -(CH ₂ ) _n - (n is an integer from 4 to 12), and R ⁴ has a structure of C _n H _2n+1 - (n is an integer from 2 to 8). In yet another embodiment, it is preferred that R ³ in the hydroxylated fatty acid is an alkylene group having 7 carbon atoms (-(CH ₂ ) ₇ -), and R ⁴ is an alkyl group having 5 carbon atoms (CH ₃ CH ₂ CH ₂ CH ₂ CH ₂ -).

Specific examples of hydroxylated fatty acids of the present invention include hydroxyoctadecenoic acids. For example, hydroxyoctadecenoic acids include, but are not limited to, 9,10,13-trihydroxy-11-octadecenoic acid and/or 9,12,13-trihydroxy-10-octadecenoic acid and their isomers.

As the derivative of hydroxylated fatty acid, ester is preferable. Examples of ester of hydroxylated fatty acid of the present invention include, but are not limited to, methyl ester, ethyl ester, propyl ester, butyl ester, pentyl ester, isopentyl ester, octyl ester, etc. In addition, examples of salt of hydroxylated fatty acid include ammonium salt such as alkyl ammonium salt such as ammonium salt, tetramethylammonium salt, etc., alkaline earth metal salt such as calcium salt, magnesium salt, etc., alkali metal salt such as sodium salt, lithium salt, potassium salt, etc., metal salt such as cobalt salt, manganese salt, etc. However, there is no particular limitation as long as it is one or more kinds of salt that are agriculturally acceptable, such as salts contained in fertilizers, etc.

The unsaturated fatty acid (excluding unsaturated oxo fatty acids and unsaturated hydroxylated fatty acids) of the present invention is not particularly limited, but unsaturated fatty acids having about 10 to 20 carbon atoms may be preferred from the viewpoint of low solubility in water. For example, unsaturated fatty acids having 18 carbon atoms are more preferable. In addition, the number of unsaturated bonds in the molecular structure of the unsaturated fatty acid of the present invention is not limited, and it may be a monounsaturated fatty acid or a polyunsaturated fatty acid. For example, the unsaturated fatty acid may be, but is not limited to, unsaturated fatty acids such as oleic acid, linoleic acid, oleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. In addition, the unsaturated fatty acid may be in the form of either a free fatty acid or a salt thereof (a salt that can be generally used, for example, the salts exemplified above), and one or more of these may be selected. As the unsaturated fatty acid of the present invention, a commercially available product may be used, or it may be a synthetic product from a commercially available compound. In addition, as the unsaturated fatty acid, one type of unsaturated fatty acid may be used, or two or more types may be used.

In addition, when isomers exist in the compounds exemplified in this specification, all possible isomers can be used in the present invention unless otherwise specified.

The fertilizer component absorption promoter for plants of the present invention contains at least one unsaturated oxo fatty acid or a derivative or a salt thereof, and if desired, further contains at least one compound selected from at least one hydroxylated fatty acid or a derivative or a salt thereof. In more preferred cases, the fertilizer component absorption promoter for plants of the present invention contains at least one unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid).

The plant fertilizer component absorption promoter of the present invention may contain at least one compound selected from at least one oxo fatty acid selected from 13-oxoODA and 9-oxoODA, or a derivative or a salt thereof, and at least one hydroxylated fatty acid selected from 9,10,13-trihydroxy-11-octadecenoic acid and/or 9,12,13-trihydroxy-10-octadecenoic acid, or a derivative or a salt thereof, and at least one unsaturated fatty acid selected from oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. For example, the plant fertilizer component absorption promoter of the present invention may contain at least one compound selected from ketooctadecadienoic acid, or a derivative or a salt thereof, and hydroxyoctadecenoic acid, or a derivative or a salt thereof, and at least one unsaturated fatty acid selected from oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. For example, the plant fertilizer component absorption promoter of the present invention may contain 13-oxo-9,11-octadecadienoic acid or a derivative or a salt thereof and linoleic acid, may contain 9,10,13-trihydroxy-11-octadecenoic acid and/or 9,12,13-trihydroxy-10-octadecenoic acid or a derivative or a salt thereof and linoleic acid, or may contain 13-oxo-9,11-octadecadienoic acid and/or 9,10,13-trihydroxy-11-octadecenoic acid and/or 9,12,13-trihydroxy-10-octadecenoic acid or a derivative or a salt thereof and linoleic acid.

In the present invention, when mixing at least one unsaturated oxo fatty acid or a derivative or a salt thereof with at least one hydroxylated fatty acid or a derivative or a salt thereof, which is added as necessary, and at least one unsaturated fatty acid, which is added as necessary, it is desirable to use at least one compound selected from the at least one unsaturated oxo fatty acid or a derivative or a salt thereof, the at least one hydroxylated fatty acid or a derivative or a salt thereof, and a stabilizer (emulsifier) for stably maintaining the at least one unsaturated fatty acid as an emulsion in water in order to mix them uniformly.

As the stabilizer (emulsifier), at least one selected from potassium carbonate and dipotassium hydrogen phosphate can be used.

As described above, the plant fertilizer component absorption promoter of the present invention contains an unsaturated oxo fatty acid or a derivative or a salt thereof, and optionally a hydroxylated fatty acid or a derivative or a salt thereof, and optionally an unsaturated fatty acid. These unsaturated fatty acids and hydroxylated fatty acids can also exist in nature, so the plant fertilizer component absorption promoter of the present invention is also extremely excellent in that it places little burden on the environment.

In the plant fertilizer component absorption promoter of the present invention, the content ratio of the unsaturated oxo fatty acid or its derivative or its salt, and the hydroxylated fatty acid or its derivative or its salt contained as necessary is preferably "unsaturated oxo fatty acid or its derivative or its salt"/"hydroxylated fatty acid or its derivative or its salt" = 1/5 to 5/1 by weight, and more preferably 1/3 to 3/1. This is because it has excellent fertilizer absorption promoting effects and plant growth promoting effects.

It is preferable that the unsaturated oxo fatty acid or its derivative or its salt, and the hydroxylated fatty acid or its derivative or its salt added as needed, are contained in the plant fertilizer component absorption promoter of the present invention at a weight ratio of 1% or more relative to the unsaturated fatty acid. This is because the unsaturated oxo fatty acid or its derivative or its salt, and the hydroxylated fatty acid or its derivative or its salt added as needed, in the plant fertilizer component absorption promoter, are effectively prevented from being washed off.

The plant fertilizer component absorption promoter of the present invention may contain an excipient or carrier as necessary. These added components are not particularly limited as long as they are agriculturally acceptable drugs. In addition, other than the excipient or carrier, components that are normally used in pesticide formulations may also be contained.

The plant fertilizer component absorption promoter of the present invention can be applied to plants by any method. For example, it can be used as a wettable powder suspended in water, and may be used as a spray to be brought into contact with the roots of a plant. The plant fertilizer component absorption promoter of the present invention may be used as a dipping agent or a soil irrigation agent. The specific application method can be appropriately selected depending on the cultivated plant to be applied and the form of use, and examples of the application method include ground liquid spraying, ground solid spraying, aerial liquid spraying, aerial solid spraying, liquid surface spraying, application within a facility, soil mixing application, soil irrigation application, surface treatment such as coating treatment, application in a nursery box, single flower treatment, and base treatment. The plant fertilizer component absorption promoter of the present invention may be mixed with the fertilizer components of the plant and used as a plant fertilizer.

The fertilizer components for the plant are not limited to any natural or synthetic chemicals that function as a source of macronutrients and/or micronutrients for the plant.

The macronutrients include at least one selected from the group consisting of nitrogen (N), phosphorus (P) and potassium (K). The micronutrients include at least one selected from the group consisting of calcium (Ca), magnesium (Mg), sulfur (S), zinc (Zn), iron (Fe), manganese (Mn), boron (B), Co (cobalt), molybdenum (Mo), copper (Cu) and Ni (nickel).

Specifically, the following chemicals can be used as fertilizer components for the plants:

Examples of nitrogen fertilizers that can be used include ammonium chloride, ammonium sulfate, ammonium nitrate, ammonium dihydrogen phosphate, urea, and lime nitrogen. Examples of phosphorus fertilizers that can be used include triple superphosphate (calcium dihydrogen phosphate), superphosphate (a mixture of calcium dihydrogen phosphate and calcium sulfate), dipotassium hydrogen phosphate, and fused phosphorus fertilizer. Examples of potassium fertilizers that can be used include potassium nitrate, potassium chloride, potassium sulfate, potassium carbonate, and potassium silicate.

Examples of magnesium fertilizers include magnesium sulfate and magnesium chloride, examples of calcium fertilizers include quicklime, hydrated lime, and calcium carbonate, examples of manganese fertilizers include manganese sulfate, manganese sulfate, and manganese tailings, examples of boron fertilizers include boric acid and borates, examples of iron fertilizers include steel slag, examples of copper fertilizers include copper sulfate, examples of cobalt nitrate and cobalt sulfate, examples of zinc fertilizers include zinc sulfate, zinc sulfide, and zinc oxide, examples of nickel fertilizers include nickel chloride, examples of molybdenum fertilizers include sodium molybdate, and examples of sulfur fertilizers include flowers of sulfur.

When the plant fertilizer component absorption promoter of the present invention is used in a mixture with such plant fertilizer components, it may be applied to the plant using an application method suitable for each fertilizer component.

The plants to which the plant fertilizer component absorption promoter of the present invention can be applied are not particularly limited, and can be used effectively on plants in general. For example, it can be suitably applied to plants of the Brassicaceae, Poaceae, Leguminosae, Solanaceae, Rosaceae, Amaranthaceae, or Malvaceae families. In addition, the plants to which it can be applied are not limited to wild-type plants, and may be, for example, mutants or transformants. In addition, the varieties of each plant are not particularly limited.

The present invention will be described based on examples, but the present invention is not limited to only the examples.

[Example 1]
As the oxo fatty acid or a derivative or salt thereof, 13-oxo-9,11-octadecadienoic acid (13-oxoODA) ((9Z,11E)-13-oxo-9,11-octadecadienoic acid, manufactured by Cayman Chemical Co.), which is a ketooctadecadienoic acid, was used. As the hydroxylated fatty acid or a derivative or salt thereof, a mixture of hydroxyoctadecenoic acid, 9,10,13-trihydroxy-11-octadecenoic acid (Larodan Fine Chemicals, 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid (English: 9(S),10(S),13(S)-trihydroxy-11(E)-octadecenoic acid)) and 9,12,13-trihydroxy-10-octadecenoic acid (Larodan Fine Chemicals, 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid (English: 9(S),12(S),13(S)-trihydroxy-10(E)-octadecenoic acid)) in a ratio of 2:1 was used. Linoleic acid (primary linoleic acid manufactured by Fujifilm Wako Pure Chemical Industries, Ltd.) was used as the unsaturated fatty acid. 0.09 g of 13-oxoODA, 0.02 g of 9,10,13-trihydroxy-11-octadecenoic acid, 0.01 g of 9,12,13-trihydroxy-10-octadecenoic acid, and 0.5 g of dipotassium hydrogen phosphate were added to 1 g of linoleic acid and mixed. This mixture was dispersed in water to prepare 16 L of an aqueous solution (aqueous dispersion) as a fertilizer component absorption promoter stock solution.

Rice (variety: Nipponbare) seeds were soaked in water for 5 days in a weather machine (LH-60FL3-DT: manufactured by Nippon Medical and Chemical Equipment Manufacturing Co., Ltd.) at a temperature of 15°C with the lights off, and then the temperature was raised to 30°C for half a day. The resulting pigeon-breasted seeds were sown in a 72-hole cell tray containing sterilized seed sowing soil (manufactured by Takii Seed Co., Ltd.), four per cell. They were grown in the weather machine in a daily cycle of 14 hours under fluorescent lights at a temperature of 28°C and 10 hours under lights at a temperature of 23°C until they reached the 1 to 1.5 leaf stage.

The fertilizer absorption promoter stock solution was diluted 100 times with water and sprayed on the leaf surface of rice plants 7 days after sowing using a spray bottle, at 1 mL per plant, on 20 plants.

Three hours and 24 hours after spraying, five samples (two plants per sample) were cut from the above-ground parts and weighed (fresh weight: FW) in 15 mL centrifuge tubes with lids, and immediately transferred to a -80°C freezer and frozen for 24 hours.

A mixture of ethanol:water:acetic acid = 80:20:1 was added to the frozen sample to a concentration of 0.1 g/1 mL, and the beads were crushed, followed by ultrasonic treatment for 10 minutes. This was left to stand for 1 hour, and then centrifuged at 3000 rpm for 5 minutes in a centrifuge (himac CT6E: manufactured by Eppendorf Himac Technologies Co., Ltd.). The supernatant was filtered through a membrane filter to prepare the analysis sample.

The analytical samples were analyzed using an LC-MS/MS device (LC section: DIONEX Ultimate 3000, MS/MS section: Q Exactive Focus: Thermo Fisher Scientific Co., Ltd.) under the following conditions: Column = Acclaim PR-MS 2.1 mmφ x 150 mm (Thermo Fisher Scientific Co., Ltd.), Solvent = 2% acetonitrile/acetic acid water → 95% acetonitrile/acetic acid water, Flow rate = 0.25 mL/min, Column temperature = 40°C, Detection = MS-(SIM), Introduction = 2 μL of sample liquid. Auxin was prepared using a calibration curve of the analytical value using 3-indoleacetic acid (a first-class reagent manufactured by Fujifilm Wako Pure Chemical Co., Ltd.), and quantification was performed from the peak area value of MS-. The auxin content in the rice sample was 4.7 ng/gFW 3 hours after spraying the fertilizer absorption promoter, and 5.9 ng/gFW 24 hours after spraying.

[Comparative Example 1]
Seven days after sowing, rice plants were grown in the same manner as in Example 1, and 1 mL of water was sprayed on the leaf surfaces of 20 plants using a spray bottle.

Samples were collected, extracted, and analyzed in the same manner as in Example 1, and the amount of auxin contained was quantified. As a result, the auxin content in the rice sample 3 hours after water spraying was 3.9 ng/gFW, and the auxin content in the rice sample 24 hours after water spraying was 3.8 ng/gFW.

[Example 2]
In the same manner as in Example 1, a stock solution of the fertilizer component absorption promoter was prepared.

The fertilizer component absorption promoter stock solution was diluted 50 times with water (Solution A). 21.4 g of urea, 13.4 g of potassium chloride, and 44.9 g of dipotassium hydrogen phosphate (N:P:K = 10:8:27) were dissolved in 1 L of water and further diluted 3000 times with water (Solution B). 100 mL of Solution A and 100 mL of Solution B were mixed and sprayed with a spray bottle on the leaf surface of rice plants 7 days after sowing, at 1 mL per plant, on 20 plants.

Samples were collected, extracted, and analyzed in the same manner as in Example 1, and the amount of auxin contained was quantified. As a result, the auxin content in the rice sample 3 hours after water spraying was 6.6 ng/gFW, and the auxin content in the rice sample 24 hours after water spraying was 7.6 ng/gFW.

[Comparative Example 2]
50 mg of stearic acid (manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd.) and 150 mg of polyoxyethylene (20) sorbitan monooleate (manufactured by FUJIFILM Wako Pure Chemical Industries, Ltd.) were dispersed in water to prepare 1 L of an aqueous solution (aqueous dispersion) as a comparative liquid.

Rice seeds were sown in the same manner as in Example 1, and grown in a weather chamber with a daily cycle of 14 hours at 28°C under fluorescent lights and 10 hours at 23°C with the lights off, and then the comparative liquid was sprayed.

Samples were collected, extracted, and analyzed in the same manner as in Example 1, and the amount of auxin contained was quantified. As a result, the auxin content in the rice sample 3 hours after water spraying was 3.0 ng/gFW, and the auxin content in the rice sample 24 hours after water spraying was 3.1 ng/gFW.

As shown in Table 1, it was found that spraying the fertilizer component absorption promoter of the present invention increased the auxin content in plants. Therefore, it is believed that in plants to which the fertilizer component absorption promoter has been applied, the absorption of fertilizer components such as potassium is promoted through activation of the cell membrane proton pump by auxin, and the elongation and growth of the plant is induced. On the other hand, in Comparative Example 1, in which only water was sprayed, and Comparative Example 2, in which a comparative liquid containing stearic acid, a linear saturated fatty acid, was sprayed, no increase in the auxin content in the applied plants was observed. In Example 2, in which the fertilizer component absorption promoter of the present invention was applied together with commercially available fertilizer components, the auxin content in the plant was further increased. Therefore, by using the fertilizer component absorption promoter of the present invention together with the fertilizer components of the plant, a plant fertilizer having an excellent plant growth promoting effect with improved absorption efficiency of the fertilizer components can be provided.

Claims (7)

A fertilizer component absorption promoter for plants, comprising an unsaturated oxo fatty acid or a derivative or a salt thereof ,
the unsaturated oxo fatty acid is 13-oxo-9,11-octadecadienoic acid;
A fertilizer component absorption promoter for plants, wherein the fertilizer component for plants is phosphorus or potassium . The plant fertilizer component absorption promoter further contains a hydroxylated fatty acid or a derivative or a salt thereof, and the hydroxylated fatty acid is 9,10,13-trihydroxy-11-octadecenoic acid or 9,12,13-trihydroxy-10-octadecenoic acid. The plant fertilizer component absorption promoter according to claim 1. The plant fertilizer component absorption promoter according to claim 1 or 2, further comprising an unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid). 4. The fertilizer component absorption promoter for plants according to claim 3 , wherein the unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid) is an unsaturated fatty acid having 10 to 20 carbon atoms. 5. The plant fertilizer component absorption promoter according to claim 3 or 4 , wherein the unsaturated fatty acid (excluding unsaturated oxo fatty acid and unsaturated hydroxylated fatty acid) is a mono- or polyunsaturated fatty acid including at least one selected from the group consisting of oleic acid, linoleic acid, α-linolenic acid, γ-linolenic acid, and arachidonic acid. 6. The plant fertilizer component absorption promoter according to claim 3 , wherein the unsaturated fatty acid is linoleic acid. A plant fertilizer comprising the plant fertilizer component absorption promoter according to any one of claims 1 to 6 and the plant fertilizer component.