JP7790876B2 - Emulsifying composition - Google Patents

Description

NPMD NITE P-02114 NPMD NITE P-02115

The present invention relates to an emulsification composition.

Surfactants are used in many industrial fields, including pharmaceuticals, cosmetics, and food. Many surfactants are produced industrially through chemical reactions using fatty acids produced by the hydrolysis of fats and oils as raw materials. However, there are also surfactants produced by living organisms. Among these surfactants, those produced extracellularly by microorganisms are called biosurfactants. Biosurfactants are generally known to be low in irritation to the skin, easily degradable, and have high surface activity due to their low critical micelle concentration.

For example, Patent Document 1 reports a method for producing a biosurfactant from tetrachloroethylene using microorganisms in activated sludge.

Japanese Patent Application Laid-Open No. 2016-174537

The present invention aims to provide a novel emulsifying composition derived from microorganisms.

As a result of extensive research, the inventors discovered that the above problems could be solved by the following means, leading to the completion of the present invention.

A first aspect of the present invention is a composition for emulsification, comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture,
the microorganism is Cupriavidus sp. strain NNM23 (accession number NITE P-02114) (also simply referred to as "strain NNM23" herein) and/or Cupriavidus sp. strain NNM27 (accession number NITE P-02115) (also simply referred to as "strain NNM27" herein),
The culture is a composition for emulsification obtained by culturing the microorganism in a medium containing a compound represented by the following formula (I) and/or formula (II):

In the formula, R ¹ is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, R ² is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, A ¹ and A ² are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

A second aspect of the present invention comprises culturing a microorganism of the genus Cupriavidus in a medium containing a compound represented by the following formula (I) and/or formula (II):
The method for producing an emulsifying composition is characterized in that the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and/or Cupriavidus sp. NNM27 strain (accession number NITE P-02115):

In the formula, R ¹ is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, R ² is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, A ¹ and A ² are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

The present invention provides a novel emulsifying composition derived from microorganisms.

1 shows photographs showing the state of culture solutions in Examples and Comparative Examples.

The following describes one embodiment of the present invention. The present invention is not limited to the following embodiment.

In this specification, the range "X to Y" means "X or more and Y or less." Unless otherwise specified, operations and measurements of physical properties are performed at room temperature (20-25°C) and a relative humidity of 40-50% RH.

<Emulsifying composition>
A first aspect of the present invention is a composition for emulsification, comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture,
the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and/or Cupriavidus sp. NNM27 strain (accession number NITE P-02115);
The culture is a composition for emulsification obtained by culturing the microorganism in a medium containing a compound represented by the following formula (I) and/or formula (II):

In the formula, R ¹ is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, R ² is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, A ¹ and A ² are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

In the present invention, the microorganism of the genus Cupriavidus is Cupriavidus sp. strain NNM23 (accession number NITE P-02114) and/or Cupriavidus sp. strain NNM27 (accession number NITE P-02115).

It is preferable to use at least the NNM27 strain as the microorganism used in the present invention.

The Cupriavidus sp. NNM23 strain was deposited on September 4, 2015, at the Patent Microorganisms Depositary of the National Institute of Technology and Evaluation (2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture), and its accession number is NITE P-02114.

Cupriavidus sp. NNM27 strain was deposited at the Patent Microorganisms Depositary Center, National Institute of Technology and Evaluation (2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture) on September 4, 2015, and its accession number is NITE P-02115.

In the present invention, a culture of a microorganism of the genus Capriavidus refers to a culture medium in which a microorganism of the genus Capriavidus is cultured and a supernatant obtained by centrifuging the culture medium.

The culture of the present invention is obtained by culturing the NNM23 strain and/or the NNM27 strain in a medium containing a compound represented by the following formula (I) and/or formula (II):

In the formula, R ¹ is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, R ² is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, A ¹ and A ² are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

In the above formula (I), the saturated or unsaturated hydrocarbon group preferably has 10 to 16 carbon atoms, and more preferably 10 to 14 carbon atoms.

In the above formula (II), the saturated or unsaturated hydrocarbon group preferably has 13 to 19 carbon atoms, and more preferably 15 to 19 carbon atoms.

In the above formulas (I) and (II), the saturated or unsaturated hydrocarbon group may be linear or branched.

Examples of saturated hydrocarbon groups include n-nonyl, n-decyl, n-undecyl, n-dodecyl, n-tridecyl, n-tetradecyl, n-pentadecyl, n-hexadecyl, n-heptadecyl, n-octadecyl, n-nonadecyl, and n-eicosyl groups.

Examples of unsaturated hydrocarbon groups include nonenyl, decenyl, undecenyl, dodecenyl, tridecenyl, tetradecenyl, pentadecenyl, hexadecenyl, heptadecenyl, octadecenyl, nonadecenyl, and eicosenyl groups.

When ^R2 is a saturated hydrocarbon group, examples of the group represented by ^R2 -CO- include a decanoyl group, an undecanoyl group, a dodecanoyl group (lauroyl group), a tridecanoyl group, a tetradecanoyl group (myristoyl group), a pentadecanoyl group, a hexadecanoyl group (palmitoyl group), a heptadecanoyl group, an octadecanoyl group (stearoyl group), a nonadecanoyl group, and an eicosanoyl group.

When R ² is an unsaturated hydrocarbon group, examples of the group represented by R ² —CO— include an oleoyl group, a linoleoyl group, an α-linolenoyl group, a γ-linolenoyl group, and a palmitrenoyl group.

In the above formulas (I) and (II), A ¹ and A ² are preferably ethylene groups.

In the above formula (I), when m is an integer of 2 or more, A ¹ can independently be an ethylene group, a propylene group, or a butylene group.

In the above formula (II), when n is an integer of 2 or more, ^A2 can independently be an ethylene group, a propylene group, or a butylene group.

In the above formula (I), m is preferably an integer from 1 to 50, more preferably an integer from 1 to 30, even more preferably an integer from 1 to 8, and particularly preferably an integer from 1 to 3.

In the above formula (II), n is preferably 0.

Examples of compounds represented by the above formula (I) include ethylene glycol monodecyl ether, diethylene glycol monodecyl ether, triethylene glycol monodecyl ether, propylene glycol monodecyl ether, butylene glycol monodecyl ether, ethylene glycol monoundecyl ether, diethylene glycol monoundecyl ether, triethylene glycol monoundecyl ether, propylene glycol monoundecyl ether, butylene glycol monoundecyl ether, ethylene glycol monododecyl ether, diethylene glycol monododecyl ether, triethylene glycol monododecyl ether, and propylene glycol monododecyl ether. ether, butylene glycol monododecyl ether, ethylene glycol monotridecyl ether, diethylene glycol monotridecyl ether, triethylene glycol monotridecyl ether, propylene glycol monotridecyl ether, butylene glycol monotridecyl ether, ethylene glycol monotetradecyl ether, diethylene glycol monotetradecyl ether, triethylene glycol monotetradecyl ether, propylene glycol monotetradecyl ether, butylene glycol monotetradecyl ether, ethylene glycol monopentadecyl ether, diethylene glycol monopentadecyl ether, triethylene glycol monopentadecyl ether, propylene glycol monopentadecyl ether, butylene glycol monopentadecyl ether, ethylene glycol monohexadecyl ether, diethylene glycol monohexadecyl ether, triethylene glycol monohexadecyl ether, propylene glycol monohexadecyl ether, butylene glycol monohexadecyl ether, ethylene glycol monoheptadecyl ether, diethylene glycol monoheptadecyl ether, triethylene glycol monoheptadecyl ether, propylene glycol monoheptadecyl ether, butylene glycol monoheptadecyl ether, ethylene glycol monooctadecyl ether, diethylene glycol Examples of such ethers include ethylene glycol monooctadecyl ether, triethylene glycol monooctadecyl ether, propylene glycol monooctadecyl ether, butylene glycol monooctadecyl ether, ethylene glycol monononadecyl ether, diethylene glycol monononadecyl ether, triethylene glycol monononadecyl ether, propylene glycol monononadecyl ether, butylene glycol monononadecyl ether, ethylene glycol monoeicosyl ether, diethylene glycol monoeicosyl ether, triethylene glycol monoeicosyl ether, propylene glycol monoeicosyl ether, and butylene glycol monoeicosyl ether.

Examples of compounds represented by formula (II) above include saturated fatty acids such as decanoic acid (capric acid), undecanoic acid, dodecanoic acid (lauric acid), tridecanoic acid, tetradecanoic acid (myristic acid), pentadecanoic acid (pentadecylic acid), hexadecanoic acid (palmitic acid), heptadecanoic acid (margaric acid), octadecanoic acid (stearic acid), nonadecanoic acid, and eicosanoic acid (arachidic acid); monounsaturated fatty acids such as myristoleic acid, palmitoleic acid, sapienic acid, oleic acid, elaidic acid, and vaccenic acid; diunsaturated fatty acids such as linoleic acid; and triunsaturated fatty acids such as α-linolenic acid, γ-linolenic acid, pinoleic acid, α-eleostearic acid, and β-eleostearic acid.

The medium used to culture the microorganism of the present invention is not particularly limited, as long as it essentially contains the compound represented by formula (I) and/or formula (II) above and allows the microorganism to grow and proliferate. The medium used to culture the microorganism may be a liquid medium or a solid medium. Liquid medium is preferred because it allows the components with emulsifying properties to be easily recovered.

Components contained in the medium include the compounds represented by formula (I) and/or formula (II) above, a nitrogen source, inorganic salts, and other components.

Specific examples of compounds represented by formula (I) and/or formula (II) are as described above.

The compounds represented by formula (I) and/or formula (II) above may be added alone or in the form of a mixture of two or more types.

For example, materials containing two or more compounds represented by formula (II) include oils and fats such as safflower oil, olive oil, canola oil (rapeseed oil), perilla oil, coconut oil, sesame oil, rice bran oil, rice bran oil, soybean oil, corn oil, palm oil, palm kernel oil (coconut oil), sunflower oil, cottonseed oil, and peanut oil.

In the cultivation of microorganisms according to the present invention, the compounds represented by formula (I) and/or formula (II) correspond to carbon sources. The medium used to cultivate microorganisms may contain carbon sources other than the compounds represented by formula (I) and/or formula (II), but it is preferable that the compounds represented by formula (I) and/or formula (II) are used as the only carbon source (single carbon source).

The amount of the compound represented by formula (I) and/or formula (II) is, for example, 0.02 to 20.0 w/v%, preferably 0.1 to 10.0 w/v%, more preferably 0.1 to 5.0 w/v%, and even more preferably 0.5 to 3.0 w/v%, relative to the total volume of the medium. When two or more compounds represented by formula (I) and/or formula (II) are used, this amount refers to the total amount, and in the case of the above-mentioned oils and fats, this amount refers to the mass.

Nitrogen sources include organic nitrogen sources such as meat extract, fish extract, peptone, polypeptone, tryptone, yeast extract, malt extract, soybean hydrolysate, soybean powder, casein, milk casein, casamino acids, various amino acids such as glycine, glutamic acid, and aspartic acid, corn steep liquor, and hydrolysates of other animals, plants, and microorganisms; and inorganic nitrogen sources such as ammonia, ammonium salts such as ammonium nitrate, ammonium sulfate, and ammonium chloride, nitrates such as sodium nitrate, nitrites such as sodium nitrite, and urea. One or more of the above nitrogen sources can be selected and used.

The amount of nitrogen source (or the total amount if two or more types are used) is not particularly limited, as long as it allows the microorganisms of the present invention to grow and proliferate.

Inorganic substances include magnesium, manganese, calcium, sodium, potassium, boron, cobalt, nickel, molybdenum, copper, iron, zinc, etc. There are no particular limitations on the form in which these minerals are contained in the medium. For example, for magnesium, manganese, calcium, sodium, potassium, cobalt, nickel, copper, iron, and zinc, examples include halides such as phosphate, hydrochloride, sulfate, acetate, carbonate, and chloride. Specific examples include potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium chloride, calcium chloride, iron sulfate, magnesium sulfate, copper sulfate, manganese sulfate, cobalt chloride, nickel sulfate, zinc sulfate, etc. For boron, examples include boric acid, etc. For molybdenum, examples include molybdenum oxide, etc. One or more of the above inorganic substances can be selected and used.

There are no particular restrictions on the amount of inorganic substances used (or the total amount if two or more types are used), as long as they allow the microorganisms of the present invention to grow and proliferate.

Other ingredients include solvents, pH adjusters, etc.

Water is typically used as the solvent. There are no particular limitations on the water, but examples include pure water, distilled water, deionized water, and RO water.

Examples of pH adjusters include acids such as sulfuric acid, hydrochloric acid, nitric acid, acetic acid, and citric acid; and bases such as sodium hydroxide, potassium hydroxide, sodium carbonate, and potassium carbonate.

Specific examples of media that can be used in culturing the microorganisms of the present invention include media prepared by adding the compounds represented by formula (I) and/or formula (II) described above to the inorganic salt selective media used in the examples described below.

The method for preparing the medium is not particularly limited, and any conventionally known method can be used. For example, the medium can be prepared by dissolving the compound represented by formula (I) and/or formula (II), a nitrogen source, and an inorganic substance in a solvent (e.g., water).

Culturing of the microorganisms of the present invention is not particularly limited as long as they allow the growth and proliferation of the microorganisms, and can be carried out by known methods. For example, culturing is carried out under aerobic conditions by shaking or aeration and agitation. The microorganisms may also be cultured continuously or batchwise. Culture conditions are appropriately selected depending on the composition of the medium and the culture method, and are not particularly limited as long as they allow the growth and proliferation of the microorganisms of the present invention. The culture temperature is typically 25 to 37°C. The pH of the medium suitable for culture is not particularly limited, but is preferably 5 to 11, and more preferably 6 to 10. The culture time is not particularly limited, and can be continued until emulsification of the culture solution can be confirmed. The culture time is typically about 16 to 72 hours, and preferably about 20 to 60 hours.

Prior to culturing the microorganisms of the present invention, the microorganisms may be pre-cultured. The pre-culture method is not particularly limited as long as it allows the microorganisms of the present invention to grow and proliferate, and can be carried out by known methods. For example, the method described in the Examples can be used.

The medium used for pre-culture can be any of the above-mentioned media. Carbon sources contained in the medium can be compounds other than those represented by formula (I) and formula (II). Examples of such carbon sources include organic acids and their salts such as gluconic acid, capric acid, adipic acid, malic acid, citric acid, phenyl acetate, acetic acid, lactic acid, succinic acid, glucuronic acid, and pyruvic acid; hydrocarbons such as hexadecane; natural products such as wheat and rice; alcohols such as glycerol, methanol, and ethanol; sugars; tryptone; and triethylene glycol monobutyl ether.

The above culture conditions can be referenced as appropriate for the pre-culture conditions.

In this way, a culture medium serving as the culture of the present invention can be obtained.

The resulting culture medium can be centrifuged to precipitate the bacterial cells and recover the supernatant. The recovered supernatant contains the emulsifying components produced by the microorganism of the present invention and can be used as the culture of the present invention. There are no particular limitations on the conditions for centrifugation; for example, centrifugation at 3,000 to 5,000 rpm for 3 to 10 minutes may be used.

In the present invention, the extract from a culture of a microorganism of the genus Capriavidus refers to the extract obtained by solvent extraction of the culture, its diluted solution and concentrated solution, and their dried products.

The method for extracting the extract from the culture is not particularly limited, and any conventionally known method can be used. For example, the extract can be obtained by the following method:
(Step 1) Mixing the culture with a solvent (e.g., ethyl acetate) to obtain a mixture;
(Step 2) Centrifuge the mixture (e.g., 600 rpm, 10 minutes);
(Step 3) The solvent layer is recovered, and the solvent is removed from the solvent layer using a rotary evaporator or the like to obtain an extract.

The solvent used for extraction is not particularly limited, and conventionally known solvents can be used. Examples of solvents include water; alcohols such as methanol, ethanol, propanol, and butanol; polyhydric alcohols such as propylene glycol and butylene glycol; ketones such as acetone and methyl ethyl ketone; esters such as methyl acetate and ethyl acetate; linear and cyclic ethers such as tetrahydrofuran and diethyl ether; polyethers such as polyethylene glycol; hydrocarbons such as hexane, cyclohexane, and petroleum ether; aromatic hydrocarbons such as benzene and toluene; and pyridines. The solvent is preferably an ester such as ethyl acetate, or an alcohol such as ethanol.

The amount of solvent added varies depending on the solvent used, but is typically 0.5 to 10 parts by volume per 1 part by volume of culture medium. There are no particular restrictions on the temperature during mixing, and it is typically 25 to 37°C.

The extract obtained in step 3 can be used as is. Alternatively, the extract can be diluted, concentrated, or freeze-dried using conventional methods and then used as an extract.

<Method of manufacturing emulsification composition>
A second aspect of the present invention comprises culturing a microorganism of the genus Cupriavidus in a medium containing a compound represented by the following formula (I) and/or formula (II):
The method for producing an emulsifying composition is characterized in that the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and/or Cupriavidus sp. NNM27 strain (accession number NITE P-02115):

In the formula, R ¹ is a saturated or unsaturated hydrocarbon group having 10 to 20 carbon atoms, R ² is a saturated or unsaturated hydrocarbon group having 9 to 19 carbon atoms, A ¹ and A ² are each independently an ethylene group, a propylene group, or a butylene group, and m and n are each independently an integer of 0 to 50.

In the method for producing an emulsification composition according to the present invention, the microorganisms (strains NNM23 and NNM27), the compounds represented by formula (I) and formula (II), the medium used in the culture, the method for culturing the microorganisms, and the culture and extract thereof obtained by the culture are the same as those described above, and therefore further description will be omitted.

In a preferred embodiment, the method for producing an emulsification composition according to the present invention includes removing microorganisms from the culture obtained by culturing in order to obtain the above-mentioned extract.

The method for removing microorganisms is not particularly limited, and includes conventionally known methods. For example, the culture medium is centrifuged to precipitate the bacterial cells, and the supernatant is recovered. The centrifugation conditions are as described above.

Microorganisms can also be removed by extracting an extract from the culture. The method for extracting the extract is as described above.

<Application>
The emulsifying composition according to the present invention contains an emulsifying component derived from a microorganism, and therefore can be used in the fields of cosmetics (for example, lotions, emulsions, creams, etc.), detergents, etc. Therefore, one embodiment of the present invention is a cosmetic comprising the emulsifying composition according to the present invention. Another embodiment of the present invention is a detergent comprising the emulsifying composition according to the present invention.

The present invention will be described in more detail using the following examples and comparative examples. However, the technical scope of the present invention is not limited to the following examples. Unless otherwise specified, "%" and "parts" mean "% by mass" and "parts by mass," respectively. In the following examples, unless otherwise specified, operations were performed under conditions of room temperature (20-25°C) and relative humidity of 40-50% RH.

[Preparation of preculture solution]
Cupriavidus sp. NNM27 strain was inoculated onto a solid medium (rich agar medium) from a frozen glycerol stock solution using a platinum loop, and cultured at 30°C.

The solid medium was prepared by dissolving all ingredients except agar in purified water to achieve the composition shown in Table 1 below, adding agar to a final concentration of 0.8 w/v%, sterilizing at high temperature and high pressure, and then dispensing the mixture as needed and allowing it to solidify.

After confirming growth on the solid medium, the colonies on the solid medium were inoculated into liquid medium (inorganic salt selective culture solution containing 0.5 w/v% of a single carbon source (triethylene glycol butyl ether (BTG))) and cultured under aerobic conditions at 30°C for 12 to 20 hours (shaking speed: 40 rpm) to prepare a preculture solution.

The liquid medium was prepared by dissolving each component in pure water to obtain the composition shown in Table 2 below to prepare an inorganic salt selective culture medium. BTG was then added to the inorganic salt selective culture medium to a final concentration of 0.5 w/v%, and the mixture was sterilized at high temperature and high pressure.

[Example 1]
Two mL of the preculture solution was centrifuged (3,000 rpm, 5 minutes) to collect the bacterial cells. One liter of liquid medium (an inorganic salt selective culture solution containing 0.5 w/v% of a single carbon source (diethylene glycol monododecyl ether (DDG))) was added to the collected bacterial cells, and the mixture was cultured under aerobic conditions at 30°C with shaking (shaking speed: 40 rpm).

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not observed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactants produced by the NNM27 strain.

[Example 2]
Cultivation was carried out in the same manner as in Example 1, except that the concentration of the single carbon source (DDG) was 0.1 w/v %.

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, and partial emulsification was confirmed in the upper part of the culture medium (Figure 1: Culture medium after approximately 30 hours of culturing). Since no emulsification was observed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, it is believed that the emulsification of the culture medium is due to a biosurfactant produced by the NNM27 strain.

[Example 3]
Cultivation was carried out in the same manner as in Example 1, except that safflower oil was used instead of DDG as the sole carbon source.

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not observed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactants produced by the NNM27 strain.

[Example 4]
Cultivation was carried out in the same manner as in Example 1, except that olive oil was used instead of DDG as the sole carbon source.

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not observed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactants produced by the NNM27 strain.

[Example 5]
Cultivation was carried out in the same manner as in Example 1, except that canola oil was used instead of DDG as the sole carbon source.

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked and emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Emulsification was not observed before culturing, and the liquid medium used for culturing does not contain any components with emulsifying properties, so it is thought that the emulsification of the culture medium is due to the biosurfactants produced by the NNM27 strain.

[Comparative Example 1]
Cultivation was carried out in the same manner as in Example 1, except that BTG was used instead of DDG as the sole carbon source.

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, and growth of the NNM27 strain was confirmed, but emulsification of the culture medium was not confirmed (Figure 1). Furthermore, emulsification was not confirmed before culturing either.

[Comparative Example 2]
Each component was dissolved in pure water to obtain the composition shown in Table 2 above, and then sterilized at high temperature and high pressure to prepare an inorganic salt selective culture medium.

2 mL of the preculture medium was centrifuged (3,000 rpm, 5 minutes) to collect the bacterial cells. 1 L of inorganic salt selective culture medium was added to the obtained bacterial cells, and the cells were cultured under aerobic conditions at 30°C with shaking (shaking speed: 40 rpm).

After culturing for approximately 20 to 60 hours, the state of the culture medium was checked, but no growth of the NNM27 strain or emulsification of the culture medium was confirmed (Figure 1: Culture medium after approximately 30 hours of culturing). Furthermore, emulsification was not confirmed before culturing either.

Claims (5)

1. An emulsifying composition comprising a culture of a microorganism of the genus Cupriavidus or an extract from the culture,
the microorganism is Cupriavidus sp. NNM23 strain (accession number NITE P-02114) and/or Cupriavidus sp. NNM27 strain (accession number NITE P-02115);
The culture is obtained by culturing the microorganism in a medium containing diethylene glycol monododecyl ether . A cosmetic comprising the emulsifying composition according to claim 1 . A detergent comprising the emulsifying composition according to claim 1 . The method comprises culturing a microorganism of the genus Cupriavidus in a medium containing diethylene glycol monododecyl ether ,
The method for producing an emulsification composition, wherein the microorganism is Cupriavidus sp. strain NNM23 (accession number NITE P-02114) and/or Cupriavidus sp. strain NNM27 (accession number NITE P-02115) . The method according to claim 4 , further comprising removing the microorganism from the culture obtained by the culturing.