JPS5678598A - Method for measuring enzyme immunity by simultaneous determination of two components - Google Patents
Method for measuring enzyme immunity by simultaneous determination of two componentsInfo
- Publication number
- JPS5678598A JPS5678598A JP15289579A JP15289579A JPS5678598A JP S5678598 A JPS5678598 A JP S5678598A JP 15289579 A JP15289579 A JP 15289579A JP 15289579 A JP15289579 A JP 15289579A JP S5678598 A JPS5678598 A JP S5678598A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- antigen
- beads
- enzyme
- test tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
PURPOSE: To measure the enzyme immunity in a small amount of a sample with an antigen-antibody reaction, by solidifying an antibody on the inner wall of a test tube, introducing beads in which another antibody is solidified on the surfaces thereof, a test solution and an antigen labeled with an enzyme into the test tube to carry out the antigen-antibody reaction, and removing the beads from the test tube to carry out the remaining antigen-antibody reaction.
CONSTITUTION: An antibody A is solidified on the inner wall of a test tube 1, and another antibody B is solidified on the surfaces of beads 2. The beads 2 are then introduced into the test tube 1, and a test solution 3 is added thereto. An antigen B labeled with an enzyme is then added to bond the antigen A in the test solution 3 to the antibody A on the surface of the inner wall in the test tube 1. The antigen B and the antigen B labeled with the enzyme are competitively bonded to the antibody B on the surfaces of the beads 2. The amount of the antigen B labeled with the enzyme linked to the antibody B depends upon the amount of the antibody B to be determined. After the completion of the bonding, the beads 2 are taken out and washed to measure the activity of the enzyme linked to the antibody B on the beads 2. On the other hand, the test tube 1 is washed, and the antigen A labeled with an enzyme is added and linked to the antibody A.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15289579A JPS592346B2 (en) | 1979-11-28 | 1979-11-28 | Two-component simultaneous quantitative enzyme immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15289579A JPS592346B2 (en) | 1979-11-28 | 1979-11-28 | Two-component simultaneous quantitative enzyme immunoassay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5678598A true JPS5678598A (en) | 1981-06-27 |
| JPS592346B2 JPS592346B2 (en) | 1984-01-18 |
Family
ID=15550468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP15289579A Expired JPS592346B2 (en) | 1979-11-28 | 1979-11-28 | Two-component simultaneous quantitative enzyme immunoassay |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS592346B2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5712363A (en) * | 1980-06-24 | 1982-01-22 | Daiichi Rajio Isotope Kenkyusho:Kk | Immunoassay for various simultaneous measurement |
| JPS5821565A (en) * | 1981-07-31 | 1983-02-08 | Fuji Photo Film Co Ltd | Microcapsule for detecting variety of antibody and detection method thereby |
| JPS5960260A (en) * | 1982-09-29 | 1984-04-06 | Toyobo Co Ltd | Enzyme immunological measurement |
| JPS60257363A (en) * | 1984-06-05 | 1985-12-19 | Dai Ichi Pure Chem Co Ltd | Measuring method of fdp |
-
1979
- 1979-11-28 JP JP15289579A patent/JPS592346B2/en not_active Expired
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5712363A (en) * | 1980-06-24 | 1982-01-22 | Daiichi Rajio Isotope Kenkyusho:Kk | Immunoassay for various simultaneous measurement |
| JPS5821565A (en) * | 1981-07-31 | 1983-02-08 | Fuji Photo Film Co Ltd | Microcapsule for detecting variety of antibody and detection method thereby |
| JPS5960260A (en) * | 1982-09-29 | 1984-04-06 | Toyobo Co Ltd | Enzyme immunological measurement |
| JPS60257363A (en) * | 1984-06-05 | 1985-12-19 | Dai Ichi Pure Chem Co Ltd | Measuring method of fdp |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS592346B2 (en) | 1984-01-18 |
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