JPS5811822B2 - Aquatic biological testing equipment - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an aquatic organism testing device used for toxicity tests, concentration tests, etc. by oral administration.

Currently, many methods for testing the toxicity and concentration of chemical substances, drugs, and other test substances on aquatic organisms such as fish, shellfish, frogs, and daphnia have not yet been established. When conducting various oral toxicity tests and concentration tests using aquatic organisms, for example, in addition to the oral route of ingestion of the test substance into the body, there are also other routes for the test substance to be taken into the body, such as food, feces, etc. of aquatic organisms. Various routes of uptake other than the oral route are also conceivable, such as a test substance being eluted into water, and the eluted test substance being reabsorbed through the gills, epidermis, etc., and taken into the living body.

For this reason, the relationship between the dose of the test substance administered through the feed and the amount actually taken into the body becomes unclear, and therefore the relationship between dose and effect becomes unclear, and the data obtained also becomes highly variable. .

In addition, in the multi-fish breeding method in which many aquatic organisms are kept in one test tank, it is difficult to feed each aquatic organism evenly during feeding, and therefore the amount of food consumed per fish becomes unclear. There are problems specific to aquatic organisms, such as the amount-effect relationship becoming unclear and the data becoming more dispersive as described above.

The present invention was made to improve the above-mentioned situation, and its purpose is to make the amount of food consumed per fish uniform, and to reduce the amount of test substances eluted into the water from feed and feces to aquatic organisms. By preventing reabsorption, it is possible to clarify the relationship between dose and effect, and it is also possible to reduce the variation in obtained data, making it suitable for use in toxicity tests, concentration tests, etc. using oral administration methods. Our objective is to provide aquatic biological testing equipment.

Hereinafter, one embodiment of the present invention will be described with reference to the drawings.

In the figure, 1 is a box-shaped test water tank formed by bonding thick glass plates with silicone adhesive, and the top is open.

Four right-angled triangular support plates 3 are fixed to the inner bottom wall 2 of the test water tank 1 and spaced apart from each other by a predetermined distance along the length direction, and the glass thickness is Inclined plates 4, 4 made of plates are each fixed, and an upper plate 5 made of a thick glass plate is fixed to the upper end surfaces of these inclined plates.

In addition, in this test water tank 1, two partition plates 6, 6 are provided at a predetermined distance from both walls in the width direction, with a gap 7 formed between the lower end and the inclined plate. A first stream is provided which communicates between the partition plates 6.6 and the inclined plates 4, 4 and between the partition plate 6.6 and the side wall of the water tank 1 through the gap 7. passages 8, 8 and second passages 9, 9
are formed respectively.

A glass box-shaped water supply device 10 with an open top end is fixed on the top plate 5.

A plurality of pipes (30 pipes in total in this embodiment) spaced apart from each other at equal intervals are provided on both walls 11.11 in the width direction of the water supply device 10.
A water supply pipe 12 is provided to protrude outward.

A predetermined number (six in total in this embodiment) of square frame-shaped glass rearing cages 13 are disposed above the inclined plates 4, 4 between the partition plates 6, 6 and the water supply device 10. and these baskets 1
The entire lower end opening of the baskets 13 is covered with a nylon net 14, and glass partition plates 15 are removably attached to the baskets 13 along the width direction. The interior of the breeding room 13 is further divided into a predetermined number (five in this embodiment) of breeding rooms 16.

Reference numeral 17 denotes a water tank installed above the water tank 1, in which breeding water whose temperature and amount of dissolved oxygen have been adjusted is stored, and its water level is always kept constant by adjusting it with a float valve or the like.

A rearing water outlet provided at the lower part of one side wall of the water tank 17 is connected to an inlet provided at the longitudinal side wall of the water supply device 10 by a rearing water supply pipe 18. The breeding water in the water tank 17 is supplied to the water supply device through the water tank 17, and after the water in the water supply device 10 flows to each breeding room 16 through the water supply pipe 12, it is passed from the net 14 to the first channel 8,
The liquid flows through the gap 7 and the second flow path 9 in this order.

Note that 19 is a cock that adjusts the flow rate of breeding water flowing through the feeding pipe 18, and 20 is a float meter for measuring the flow rate.

Reference numeral 21 denotes a U-shaped culture water suction pipe made of glass, which is installed above the second flow path 9 formed by the test tank 1 and the partition plate 6, and its front end is hung downward. At the same time, a breeding water suction section 22 is formed in the second flow path 9 and branched into a T-shape.

The suction pipe 21 is connected to a suction port of a pump 24 disposed in a pump storage chamber 23 formed in the test water tank 1 by a suction side pipe 25.

An arch-shaped opening is provided at the lower end of the side wall in the longitudinal direction of the water tank 1, and the suction side pipe 25
The pump 24 inside the pump storage chamber 23 passes through this opening.
It is connected with.

A discharge port of the pump 24 is connected to one end of a discharge pipe 27 which has an activated carbon column 26 filled with activated carbon interposed therein.

The other end of the discharge side pipe 27 is connected to the feed pipe 18 on the downstream side of the float meter 20 of the feed pipe 18, and the breeding water sent through the feed pipe 18 by the action of gravity and the pump The circulating water sent through the pipe 27 by the pipe 24 joins together and flows into the water supply device 10.

Note that 28 is a cook. Discharge holes 29, 29 are provided at both ends of the lower part of the other end wall of the water tank 1 in the length direction, and communicate with the second channels 9, 9.
A U-shaped connecting portion of an overflow pipe 30 is connected to this discharge hole 29, 29, and the other end almost reaches the upper end of the water tank 1.

A branch pipe 31 is installed at the other end of the overflow pipe 30 at a position slightly above the breeding water suction section 22, and the other end is connected to a wastewater treatment device (not shown) using, for example, a vinyl pipe 32. ) and is connected to tank 1.
When the water level of the rearing water in the second channel 9 becomes higher than the branch part 33 of the branch pipe 31, the rearing water in the second flow path 9 overflows through the connecting part and the overflow pipe, and then flows through the branch pipe 31 and the vinyl pipe 32. It is designed to be discharged to wastewater treatment equipment.

Next, a case will be described in which a concentration test for carp is conducted by oral administration using the aquatic organism testing device configured as described above.

First, when the cock 19 is opened and the rearing water whose temperature and dissolved oxygen amount in the water tank 1 have been adjusted is sent to the water supply device 10 via the feed pipe 18, the rearing water fills the water supply device 10 and the both sides of the rearing water are fed. The water flows out from each water supply pipe 12 provided in the water supply pipes 12, flows into each breeding chamber 16 formed below, passes through the net 14, and flows through the first flow path 8, the gap 7, and the second flow path 9. When the water level of the aquarium 1 reaches the branch part 33, the breeding water is discharged from the drain hole 2.
9. Passing through 29 is the branch part 33 of the overflow pipe 30
The water overflows and is sent to the wastewater treatment equipment through the branch pipe 31 and further through the vinyl pipe 32.

Next, when the pump 24 is activated, the breeding water in the flow path 9 passes from the breeding water suction section 22 through the suction pipe 21 to the pump 2.
4 enters the pump 24 from the suction side, and further passes from the discharge side of the pump 24 through the discharge side pipe 27 with a cock 28 interposed to reach the activated carbon column 26, and then the breeding water that has passed through the column 26 passes through the discharge side pipe 27. water flows into the feeding pipe 18 and merges with the breeding water flowing inside the pipe 18 to form the water supply device 10.
Repeat this flow.

Next, after adjusting the cock 19 to set the breeding water flow rate to a predetermined value, one test point carp 34 is placed in each breeding room 16, and the time required for the carp 34 to adapt to the new environment is determined. After a period of approximately one week, pelleted feed containing a predetermined amount of the test substance is orally administered for a predetermined period of time to study the physiological effects of the test substance on the carp. For feeding, first feed the carp 34 in the breeding room 16 with pelleted food.
After confirming that the carp 34 has ingested this pellet, the second pellet is given repeatedly, and the feeding is continued while checking the number of pellets that each carp 34 has ingested.

In this case, the food, feces, etc. left uneaten by the carp 34 are quickly moved downwards in the breeding room 16 by riding on the flow of breeding water moving from the top to the bottom in the breeding room 16, and further into the breeding room 16.
6. Breeding water passes through the net 14 stretched at the bottom and flows down along the inclined plate 4, settles on the bottom wall of the test tank 1 and is separated from the breeding water, and the uneaten food, feces, etc. are separated. Of the water, an amount of breeding water equal to the amount of breeding water sent from the water tank 17 through the feed pipe 18 is diverted to the discharge hole 2.
9 to the overflow pipe 30, and further to the branch pipe 3.
1. While being sent to the wastewater treatment device through the vinyl pipe 32, the remaining breeding water ascends through the second channels 9, 9, and is sucked from the breeding water suction section 22 by the action of the pump 24 into the suction side pipe 25; Pump 24, discharge side pipe 27, cock 2
8 and flows into the activated carbon column 26, and the activated carbon packed in this column removes various contaminants such as test substances eluted into the breeding water from the fish excrement and feed or excrement present in the breeding water. 34. After returning to the breeding water suitable for breeding, the discharge side pipe 27. The water flows into the water supply device 10 through a feed pipe 18 and is circulated therein.

The breeding water used in the apparatus of the present invention, that is, the breeding water in which aquatic organisms are raised, can be, for example, well water, river water, ion exchange water, distilled water, or seawater in some cases, but for the purpose of biological testing. The choice should be made accordingly.

In addition, it is desirable that the amount of dissolved oxygen in these rearing waters is 4 ppm or more, especially 5 ppm or more, and the COD of these waters is approximately 1 ppm or less, and the BOD is 0.5 to 1.
It is preferably about ppm or less, and needs to be substantially free of heavy metal ions and other harmful substances.

In this example, a plurality of breeding rooms are provided in the test tank 1, and one carp is bred in each of the breeding rooms, and pelleted food containing a predetermined amount of the test substance is fed to the carp. By counting the amount of pelleted food actually consumed by each carp, the oral dosage of the test substance for each carp can be determined accurately, improving the reliability of the experiment.

Since the breeding water passes through the breeding room and always flows from the top to the bottom, carp excrement, uneaten food, etc. flow out of the breeding room quickly, and the breeding room is always kept clean. This ensures the reliability of the experiment by preventing test substances that have been eluted into the rearing water from carp excrement, uneaten food, etc. from remaining in the rearing room for a long time and being reabsorbed through the carp gills, etc. to have a high level of sexuality.

In addition, since inclined plates 4, 4 are provided in the test tank 1, excrement, uneaten food, etc. that flowed out from the breeding chamber 16 are moved downward along the inclined plates 4, 4 due to the flow of breeding water and the action of gravity. It moves and collects on the lower end side of the inclined plates 4, 4, and is separated and removed from the flow of culture water and deposited on the bottom wall 2, so that the water of the pump 24 and the activated carbon column 26 does not need to be provided with a filter or the like. The device can be operated for a long period of time without clogging, and the accumulated excrement can be easily removed by periodically suctioning the vicinity of the bottom wall 2 using, for example, a siphon. It is convenient to be able to remove it.

Then, the breeding water from which the excrement etc. have been separated rises in the second flow path 9 and is sucked from the breeding water suction section 22, and the pump 2
4 to the activated carbon column 26, where the activated carbon adsorbs and removes various contaminants such as test substances dissolved in the breeding water from fish excrement and feed or excrement, and After the breeding water is returned to the water suitable for breeding, it is sent to the water supply device 10 and further supplied to each breeding room, and the test is conducted using only the inflow water from the water tank 17 without circulating the breeding water. However, by circulating the breeding water in this way and increasing the amount of breeding water flowing through the breeding room 16, the interior of the breeding room can be more reliably cleaned.

In addition, circulating the breeding water saves the breeding water.

Although a carp was used as a test point in this example, the present invention is not limited to this, and various modifications may be made without departing from the gist of the present invention.

Accordingly, the present invention provides a breeding method in which an inclined plate is disposed on the inner bottom wall of a box-shaped test tank with an open upper end, the bottom opening is covered with a net above the inclined plate, and aquatic organisms are raised inside the inclined plate. A partition plate is arranged on the side of the breeding chamber to separate it from the side wall of the aquarium by a predetermined distance so as to form a gap between the lower end of the partition plate and the inclined plate, and forming a first flow path between the inclined plate and the partition plate, and forming a second flow path communicating with the first flow through the gap between the partition plate and the side wall of the aquarium; One end of the rearing water suction pipe is connected to the upper part of the second flow path, the other end of this suction pipe is connected to the suction side of the pump, and the discharge side of the pump is connected to the water supply device via the activated carbon column. , and an overflow pipe communicating with the second flow path is provided, and the rearing water sent from the water source is supplied from the water supply device to the rearing room, and the first flow path, the gap, and the second flow path are passed through the net. Since the system is configured so that the sequentially flowing breeding water is circulated from the suction pipe through the activated carbon column to the water supply device by the operation of the pump, it is possible to orally administer the desired amount of the test substance to each aquatic organism, increasing the reliability of the test. It is of high quality.

In addition, the rearing water flows from the top to the bottom in the rearing room, keeping the inside of the rearing room clean at all times, thereby reliably preventing the test substance from being reabsorbed by the aquatic organisms through routes other than the oral route.

In addition, since the test tank is equipped with a sloped plate, uneaten food, feces, etc. do not settle along the sloped plate, but are collected on the bottom wall, separated from the rearing water, and collected. Feed etc. have the advantage of being convenient and easy to clean with a siphon or the like.

[Brief explanation of drawings]

FIG. 1 is a schematic front view showing an embodiment of the present invention, FIG. 2 is a side sectional view taken along line 1--2 in FIG. 1, and FIG. 3 is a plan view of the same side. 1... Test water tank, 4... Inclined plate, 6... Partition plate, 7...
Gap, 8...First channel, 9...Second channel, 10...Water supply device, 14...Net, 16... Breeding room, 17...Water tank,
22... Breeding water suction section, 24...-pump, 26... activated carbon column, 30... overflow pipe.

Claims (1)

[Claims] 1 A box-shaped test tank with an open top end 1 An inclined plate 4 on the inner bottom wall 2
A rearing chamber 16 is provided above the inclined plate 4, the bottom opening of which is covered with a net 14, and in which aquatic organisms 34 are reared.
A partition plate 6 is provided on the side of the rearing room 16 to be separated from the side wall of the aquarium 1 by a predetermined distance so as to form a gap 7 between the lower end of the partition plate 6 and the inclined plate 4. A first flow path 8 is formed below the chamber 16 between the inclined plate 4 and the partition plate 6, and a second flow path 9 is connected to the first flow path 8 through the gap 7. It is formed between the partition plate 6 and the aquarium side wall, and communicates one end of the breeding water suction pipe 21 with the upper part of the second flow path 9, and connects the other end of this suction pipe 21 with the suction side of the pump 24. In addition, the discharge side of the pump 24 is connected to the water supply device 10 via the activated carbon column 26, and an overflow pipe 30 communicating with the second flow path 9 is provided, so that the breeding water sent from the water source 17 is transferred to the water supply device 10. At the same time, the breeding water is supplied from the suction pipe 21 to the breeding room 16 through the activated carbon column 26 by the operation of the pump 24, and the breeding water is supplied to the breeding room 16 through the mesh 14 and sequentially through the first channel 8, the gap 7, and the second channel 9. An aquatic organism testing device characterized in that it is configured to circulate through a vessel 10.