JPS5825437B2 - Biohazard protection method using ozone - Google Patents
Biohazard protection method using ozoneInfo
- Publication number
- JPS5825437B2 JPS5825437B2 JP13384981A JP13384981A JPS5825437B2 JP S5825437 B2 JPS5825437 B2 JP S5825437B2 JP 13384981 A JP13384981 A JP 13384981A JP 13384981 A JP13384981 A JP 13384981A JP S5825437 B2 JPS5825437 B2 JP S5825437B2
- Authority
- JP
- Japan
- Prior art keywords
- ozone
- present
- safety
- biobuzzard
- sterilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 title claims description 35
- 238000000034 method Methods 0.000 title claims description 29
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 34
- 238000004659 sterilization and disinfection Methods 0.000 description 13
- 230000001954 sterilising effect Effects 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 8
- 102000053602 DNA Human genes 0.000 description 8
- 239000002351 wastewater Substances 0.000 description 7
- 238000003958 fumigation Methods 0.000 description 6
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical group O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 230000007123 defense Effects 0.000 description 4
- 238000004065 wastewater treatment Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical group NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 230000000249 desinfective effect Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229930024421 Adenine Chemical group 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical group NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010023927 Lassa fever Diseases 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000012668 chain scission Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【発明の詳細な説明】
本発明は、微生物工学、遺伝子工学などの研究に使用さ
れる安全キャビネット及び安全実験室、あるいはそれら
からの排水のバイオフ１ザードを防御するだめの新規な
方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a novel method for protecting safety cabinets and safety laboratories used in research in microbial engineering, genetic engineering, etc., or the biofusord of wastewater from them. be.
さらに詳しくいえば、本発明は、オゾンを使用してバイ
オバザード因子を滅菌、分解することにより、簡学かつ
効率よくバイオバザードを防御する方法に関するもので
ある。More specifically, the present invention relates to a simple and efficient method of protecting against biobuzzards by sterilizing and decomposing biobuzzard factors using ozone.
近年、分子生物学や遺伝子工学などの発展にともない、
遺伝子組換え技術を駆使した研究、例えば細胞内の遺伝
情報を担５ＤＮＡ（デオキシリボ核酸）を切断し、一方
別種の生物細胞のＤＮＡ（プラスミドなど）を切断して
、これらの異種のＤＮＡ同士を再結合し、この組換えＤ
ＮＡを宿主細胞（大脹菌など）に導入することにより、
自然界に存在しない遺伝子をもった生物を作るといった
研究などが盛んに行われるようになっている。In recent years, with the development of molecular biology and genetic engineering,
Research that makes full use of gene recombination technology, for example, cutting the DNA (deoxyribonucleic acid) that carries genetic information in cells, cutting the DNA (plasmid, etc.) of biological cells of different species, and recombining the DNA of these different species. and this recombinant D
By introducing NA into host cells (such as Bacillus macrophages),
Research is being actively conducted to create organisms with genes that do not exist in nature.
それに伴なって、これまで存在しなかった病原性微生物
を作り出す危険性も増大しており、そのため、この遺伝
子組換え実験には内容に応じて徹底したバイオバザード
防御対策が求められている。Along with this, the risk of creating pathogenic microorganisms that did not exist before is increasing, and therefore, thorough biobuzzard protection measures are required for genetic modification experiments depending on the content.
また、従来から実施されてきた病原虫微生物に関する研
究についても、例えばラッサ熱をひき起すような極めて
危険性の高い病原体や、あるいは未知の病原体を取り扱
う研究などがあり、バイオバザード防御対策は不可欠と
なっている。Furthermore, with regard to research on pathogenic microorganisms that has traditionally been conducted, for example, research deals with highly dangerous pathogens such as those that cause Lassa fever, or with unknown pathogens, so biobuzzard protection measures are essential. It has become.
しだがって、その対策として最近では安全キャビネット
の使用や、実験室そのものに安全対策設備を備えたＰ３
Ｐ４ レベルの実験室（組換えＤＮＡ実験指針、内閣総
理大臣、昭和５４年８月２７日付）の使用が義務づけら
れている。Therefore, recent countermeasures include the use of safety cabinets and P3 facilities equipped with safety equipment in the laboratory itself.
The use of a P4 level laboratory (recombinant DNA experiment guidelines, Prime Minister, August 27, 1978) is required.
ところで、現在バイオバザード防御施設で用いられてい
る滅菌、消毒法は、実験器具や廃棄物については高圧蒸
気法、排水については煮沸（１３０℃）法であり、また
、安全キャビネットや安全実験室の滅菌、消毒に関して
はホルマリンくん蒸法が指定されている（組換えＤＮＡ
実験指針）。By the way, the sterilization and disinfection methods currently used in biobuzzard protection facilities are the high-pressure steam method for laboratory equipment and waste, and the boiling (130°C) method for wastewater. For sterilization and disinfection, formalin fumigation is specified (recombinant DNA
experiment guidelines).
しかし々から、高圧蒸気法は小型のものには適用しうる
が、大型のものには適用が困難で、その上試験体を封じ
こめである容器の滅菌、消毒には不適当である。However, although the high-pressure steam method can be applied to small objects, it is difficult to apply to large objects, and furthermore, it is unsuitable for sterilizing and disinfecting containers in which test specimens are contained.
また排水の煮沸法についても、少量の場合にのみ使用可
能で、多量の場合は使用しにくいという欠点を有してい
る。The method of boiling waste water also has the disadvantage that it can only be used in small quantities and is difficult to use in large quantities.
一方、安全キャビネットや安全実験室内のホルマリンく
ん蒸法は、くん蒸装置を別途持ち込む必要があって簡便
ではなく、しかもホルムアルデヒドの滅菌、消毒力が比
較的強くないために、ホルムアルデヒド７０００ｐｐｍ
に６０分間さらすという高濃度、長時間の処理を要求し
ている。On the other hand, the formalin fumigation method in a safety cabinet or safety laboratory is not convenient as it requires a separate fumigation device, and the sterilizing and disinfecting power of formaldehyde is relatively weak, so the formalin fumigation method uses 7000 ppm of formaldehyde.
It requires high-concentration, long-time treatment, such as exposure to water for 60 minutes.
さらに滅菌、消毒後のホルムアルデヒドは多くの場合ハ
大気に放出されており、このことはホルムアルデヒドの
有毒性の点から問題があり、壕だ仮にホルムアルデヒド
を水に吸収させて除去するとしても、その排液の処理が
再び必要となって煩雑である。Furthermore, formaldehyde after sterilization and disinfection is often released into the atmosphere, which poses a problem in terms of the toxicity of formaldehyde. The liquid must be treated again, which is complicated.
これらの理由からこのホルマリンくん蒸法は必ずしも満
足しうる方法とはいえない。For these reasons, this formalin fumigation method cannot necessarily be said to be a satisfactory method.
また、補助的滅菌、消毒法として併用されている紫外線
照射法は、物体の陰の部分に対して有効ではなく、しか
も試験体を封じ込めている容器がガラスであれば、試験
体を破壊する恐れがあるなどの欠点を有している。In addition, ultraviolet irradiation, which is used in conjunction with supplementary sterilization and disinfection, is not effective against the shadows of objects, and if the container containing the test object is glass, there is a risk of destroying the test object. It has some disadvantages such as.
本発明者らは、このような欠点を克服すべく、操作が簡
学で効果的な新しいバイオバザード防御方法の開発につ
いて鋭意研究を重ねた結果、オゾンはバイオバザード因
子に対する滅菌、分解力が優れている上に、空気と電力
から容易に製造することができ、しかもその制御が簡学
であり、これを用いた滅菌、分解法は大気系、水系いず
れにおいても自動化が可能で操作が簡竿であること、残
留オゾンは活性炭で除去することが可能であり、大気を
汚染しないこと、及びオゾンはガス体であるため紫外線
と異なり滅菌、消毒されない部分を生ずることがなく、
また容器の中に封じ込めである試験体を損う恐れがない
ことなどの点に着目し、本発明を完成するに至った。In order to overcome these drawbacks, the present inventors have conducted extensive research into developing a new biobuzzard protection method that is simple to operate and effective, and have found that ozone has excellent sterilization and decomposition power against biobuzzard factors. In addition, it can be easily manufactured from air and electricity, and its control is simple.The sterilization and decomposition methods using it can be automated and are easy to operate in both atmospheric and water systems. , residual ozone can be removed with activated carbon and does not pollute the atmosphere, and since ozone is a gas, unlike ultraviolet rays, it does not leave any parts unsterilized or disinfected.
Furthermore, the present invention was completed by paying attention to the fact that there is no risk of damaging the test specimen, which is sealed inside the container.
、すなわち、本発明方法に従えば、安全キャビネット、
安全実験室のような密閉系空間又はそれからの排水に、
オゾンを導入し、バイオバザード因子を滅菌、分解する
ことにより、効果的なバイオバザードの防御を行うこと
ができる。, that is, according to the method of the present invention, a safety cabinet,
For closed spaces such as safety laboratories or drainage from them,
By introducing ozone to sterilize and decompose biobuzzard factors, effective biobuzzard protection can be achieved.
本発明のバイオバザード防御方法は、分子生物学や遺伝
子工学などにおける遺伝子組換え技術の駆使によってで
きた組換え体のもつ危険性や、あるいは病原性微生物特
にウィルスなどによって生ずる危険性の排除を対象とし
ており、したがって、従来の滅菌の概念を越えた強力な
滅菌、分解による防御方法である。The biobuzzard defense method of the present invention is aimed at eliminating the dangers of recombinant organisms created by making full use of genetic recombination techniques such as molecular biology and genetic engineering, or the dangers caused by pathogenic microorganisms, especially viruses. Therefore, it is a powerful sterilization and decomposition defense method that goes beyond traditional sterilization concepts.
すなわち、従来の滅菌処理においては、一定時間後の細
菌数が一定基準値以下に達しだところをもって処理終了
とみなしているが、本発明のバイオバザード防御方法に
おいてはバイオバザード因子の細胞膜やエンベロープ、
カプシドが破壊されて中にある遺伝情報を有するＤＮＡ
が破壊され、しかも破壊されたＤＮＡは再生不可能とな
る。That is, in conventional sterilization treatment, the treatment is considered to have ended when the number of bacteria reaches a certain standard value or less after a certain period of time, but in the biobuzzard protection method of the present invention, the cell membrane and envelope of the biobuzzard factor,
DNA containing genetic information found inside the capsid after it is destroyed
is destroyed, and the destroyed DNA cannot be reproduced.
本発明方法に用いるオゾンは、空気と電力から容易に製
造することができ、その制御も簡皐であり、しかもバイ
オバザード物に対して、従来用いられているホルムアル
デヒドに比べて優れた滅菌分解、不活性化力を有してい
る。The ozone used in the method of the present invention can be easily produced from air and electricity, and its control is simple. Furthermore, it has excellent sterilization and decomposition properties for biobuzzard materials compared to the conventionally used formaldehyde. It has inactivating power.
例えばタバコモザイクウィルスの核酸の場合、オゾンの
不活性化力はホルムアルデヒドのそれの約１０００倍に
近い。For example, in the case of the tobacco mosaic virus nucleic acid, the inactivating power of ozone is approximately 1000 times that of formaldehyde.
一方、ホルムアルデヒドによる遺伝子の不活性化メカニ
ズムについては、核酸の中のグアニン、シトシン、アデ
ニンなどのアミノ基がホルムアルデヒドによって、モノ
又はジメチロール誘導体に変化することが知られている
が、このように羊に核酸塩基がその類縁体に転化した程
度では、修復酵素により修復されて元の活性を再現する
可能性があることが、現在判明している。On the other hand, regarding the mechanism of gene inactivation by formaldehyde, it is known that amino groups such as guanine, cytosine, and adenine in nucleic acids are changed by formaldehyde into mono- or dimethylol derivatives; It has now been found that to the extent that a nucleobase has been converted to its analogue, it can be repaired by repair enzymes to restore its original activity.
本発明者らはオゾンによるＤＮＡ破壊メカニズムについ
て検討した結果、その破壊がグアニン塩基を中心と子る
ものであること、すなわち、グアニンは４，５位の二重
結合の開裂からさらに数種類の化合物に分解し、またチ
ミンも同程度の速さで分解し、遂には分子鎖切断に至る
との知見を得た。The present inventors investigated the mechanism of DNA destruction caused by ozone and found that the destruction occurs mainly at guanine bases, that is, guanine is further broken down into several types of compounds through the cleavage of the double bond at the 4 and 5 positions. They also found that thymine also decomposed at a similar rate, eventually leading to molecular chain scission.
このようなオゾンによるＤＮＡ破壊メカニズムではＤＮ
Ａの修復は不可能で、したがってバイオバザード防御対
策にオゾンを用いることは、殺菌不活性化の速度と効果
において極めて有効であるといえる。In this DNA destruction mechanism caused by ozone, DN
It is impossible to repair A. Therefore, the use of ozone as a biobuzzard defense measure is extremely effective in terms of the speed and effectiveness of bactericidal inactivation.
本発明方法においては、オゾンガスを密閉系の安全キャ
ビネット又は安全実験室に通ずることによって、空気中
に浮遊するバイオバザード因子や粘着、固着しているバ
イオバザード因子を容易に滅菌、分解することができ、
従来の高圧蒸気法、ホルマリンくん蒸法、紫外線照射法
による滅菌、消毒法より効果的な方法である。In the method of the present invention, by passing ozone gas through a closed safety cabinet or safety laboratory, it is possible to easily sterilize and decompose the biobuzzard factors floating in the air and the biobuzzard factors that are sticky or fixed. ,
This method is more effective than conventional sterilization and disinfection methods such as high-pressure steam, formalin fumigation, and ultraviolet irradiation.
さらに、本発明方法においては、安全キャビネットや安
全実験室からの排水にオゾンガスを通じることによって
、その中に存在しているバイオバザード物を効果的に滅
菌、分解することができる。Furthermore, in the method of the present invention, by passing ozone gas into the waste water from a safety cabinet or safety laboratory, biobuzzard substances present therein can be effectively sterilized and decomposed.
次に添付図面に従って本発明をさらに詳細に説明する。The present invention will now be described in more detail with reference to the accompanying drawings.
図面は本発明方法のフローシートの一例であって、１は
空気よりオゾンを発生させるだめのオゾン発生機、２は
安全キャビネット又は安全実験室、３はパスボックス（
安全キャビネットの場合はない）、４は安全キャビネッ
ト又は安全実験室からの排水を処理するだめの排水処理
装置及び５は排ガス中のオゾンを除去するだめの活性戻
光てん排オゾン除去装置である。The drawing is an example of a flow sheet of the method of the present invention, in which 1 is an ozone generator for generating ozone from air, 2 is a safety cabinet or safety laboratory, and 3 is a pass box (
4 is a wastewater treatment device for treating wastewater from a safety cabinet or a safety laboratory, and 5 is an activated backlight evacuation ozone removal device for removing ozone from exhaust gas.
空気をライン６よりオゾン発生機１に供給してオゾンを
発生させ、得られたオゾン含有空気はライン７を通って
安全キャビネット又は安全実験室２、パスボックス３（
安全キャビネットの場合ハない）及び排水処理装置４に
導かれ、室内や排水中に存在しているバイオバザード物
を滅菌、分解する。Air is supplied from line 6 to ozone generator 1 to generate ozone, and the ozone-containing air obtained passes through line 7 to safety cabinet or safety laboratory 2, pass box 3 (
In the case of a safety cabinet, the bio-buzzard material present in the room or in the wastewater is sterilized and decomposed by the wastewater treatment device 4.
安全キャビネット又は安全実験室、パスボックス及び排
水処理装置から出た排オゾン含有空気は、それぞれライ
ン８，９及び１０を通って排オゾン除去装置５に導かれ
、この中に充てんされている活性炭によってオゾンが除
去されたのち、無害の空気となってライン１１を通って
大気に放出される。Exhaust ozone-containing air discharged from the safety cabinet or safety laboratory, pass box, and wastewater treatment equipment is led to the exhaust ozone removal equipment 5 through lines 8, 9, and 10, respectively, and is treated with activated carbon filled therein. After the ozone is removed, it is released into the atmosphere through line 11 as harmless air.
本発明方法においては、オゾンを、密閉空間内の雰囲気
中に１１００ｐｐの濃度で導入する場合処理時間は６０
分間で十分であるが、濃度をあげれば処理時間は短縮で
きる。In the method of the present invention, when ozone is introduced into the atmosphere in a closed space at a concentration of 1100 pp, the treatment time is 60 pp.
Although one minute is sufficient, the processing time can be shortened by increasing the concentration.
本発明のオゾンによるバイオバザード防御方法は、密閉
系の安全キャビネットや安全実験室内に存在するバイオ
バザード因子や、それからの排水中に存在するバイオノ
・ザード因子を効果的に滅菌分解して完全に不活性化し
、またその防御システムは容易に自動化が可能で、操作
も極めて簡雫であるという利点がある。The bio-buzzard protection method using ozone of the present invention effectively sterilizes and decomposes the bio-buzzard factors present in closed safety cabinets and safety laboratories, as well as the bio-buzzard factors present in the waste water from them, and completely renders them ineffective. It has the advantage that the activation and defense system can be easily automated and is extremely simple to operate.
次に実施例により本発明をさらに詳細に説明する。Next, the present invention will be explained in more detail with reference to Examples.
実施例
安全キャビネット大の密閉チェンバー（１×１×１ｍ）
の底面に大腸菌を散布したシャレー（直径１２ｃｆｆＬ
）数個を静置した後、オゾン０．３ｍｇ／ｌ（１４０ｐ
ｐｍ）又は１．Ｏｍｇ／ｌ（４７０ｐｐｍ）を含む空気
を流速１．５ ｍ３／ｍ ｉ ｎで送りこみ、一定時間
後、清浄空気を１０分間通じ、チェンバー内のオゾンを
完全に追い出した後、シャーレをとり出し、３７℃、１
夜培養して、その生存数を測定した。Example Safety cabinet-sized sealed chamber (1 x 1 x 1 m)
A chalet (diameter 12cffL) with E. coli sprinkled on the bottom of the
) After leaving several pieces still, ozone 0.3mg/l (140p
pm) or 1. Air containing Omg/l (470 ppm) was sent in at a flow rate of 1.5 m3/min, and after a certain period of time, clean air was passed through the chamber for 10 minutes to completely drive out the ozone in the chamber, and then the petri dish was taken out. 37℃, 1
The cells were cultured overnight and the number of viable cells was measured.
大腸菌散布のサンプルシャーレは次のＡ、Ｂの２通りを
用いた。The following two sample petri dishes, A and B, were used for E. coli dispersion.
Ａ：寒天培地に大腸菌懸濁液１００μｌをのせ、ガラス
棒で表面−面に拡げた後、３７℃、１時間培養したもの
Ｂ：Ａと同様に大腸菌を寒天培地−面に拡げた後その上
に薄く寒天培地をのせ（厚さ０．０２ｍｍ）大腸菌をと
じこめた後、３７℃、１時間培養したもの
オゾンを１４０ｐｐｍ導入した場合に得られたオゾン接
触時間と生存率との関係を第２図に示す。A: 100 μl of E. coli suspension was placed on the agar medium, spread on the surface with a glass rod, and then cultured at 37°C for 1 hour B: E. coli was spread on the agar medium in the same manner as in A, and then placed on top of it. Figure 2 shows the relationship between ozone contact time and survival rate obtained when 140 ppm of ozone was introduced. Shown below.
この図から明らかなように、オゾンとの接触を困難にし
た極端な条件Ｂにおいても、従来用いられているホルム
アルデヒドによる滅菌よりも低濃度、短時間で滅菌しう
る。As is clear from this figure, even under extreme condition B in which contact with ozone is made difficult, sterilization can be achieved at a lower concentration and in a shorter time than the conventionally used formaldehyde sterilization.
まだ、サンプルＡについて、オゾン濃度を１４０ｐｐｍ
（実線）及び４７０ｐｐｍ（破線）において同様に試験
した結果を第３図に示す。Still, for sample A, the ozone concentration was set to 140 ppm.
(solid line) and 470 ppm (broken line). The results of the same test are shown in FIG.
この図から明らかなように、オゾン濃度の高い方が処理
時間を短縮することができる。As is clear from this figure, the higher the ozone concentration, the shorter the processing time.
第１図は本発明方法のフローシート、第２図及び第３図
は本発明の実施例におけるオゾン接触時間と大腸菌の生
存率の関係を示すグラフである。
図中符号１はオゾン発生機、２は安全キャビネット又は
安全実験室、３はパスボックス、４は排水処理装置、５
は除去装置である。FIG. 1 is a flow sheet of the method of the present invention, and FIGS. 2 and 3 are graphs showing the relationship between ozone contact time and E. coli survival rate in Examples of the present invention. In the figure, 1 is an ozone generator, 2 is a safety cabinet or safety laboratory, 3 is a pass box, 4 is a wastewater treatment device, 5
is a removal device.
Claims (1)
いて、該空間内にオゾンを導入し、バイオバザード因子
を滅菌、分解することを特徴とする方法。1. A method for protecting bio-buzzards in a closed system space, which comprises introducing ozone into the space to sterilize and decompose bio-buzzard factors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13384981A JPS5825437B2 (en) | 1981-08-25 | 1981-08-25 | Biohazard protection method using ozone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13384981A JPS5825437B2 (en) | 1981-08-25 | 1981-08-25 | Biohazard protection method using ozone |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP894584A Division JPS6037977A (en) | 1984-01-20 | 1984-01-20 | Apparatus for protecting from biohazard |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5836383A JPS5836383A (en) | 1983-03-03 |
| JPS5825437B2 true JPS5825437B2 (en) | 1983-05-27 |
Family
ID=15114464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13384981A Expired JPS5825437B2 (en) | 1981-08-25 | 1981-08-25 | Biohazard protection method using ozone |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5825437B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0652494A (en) * | 1992-07-30 | 1994-02-25 | Mitsubishi Electric Corp | Bus operation management device |
| CN101966421B (en) * | 2010-09-03 | 2012-05-23 | 清华大学 | A method for optimizing the long-term operation performance of VOC gas biological filter device by using ozone |
| US10696937B2 (en) | 2015-03-31 | 2020-06-30 | Thrive Bioscience, Inc. | Cell culture incubators with integrated cell manipulation systems |
| JP6851635B2 (en) * | 2015-03-31 | 2021-03-31 | スライブ バイオサイエンス， インコーポレイテッド | Automatic cell culture incubator |
-
1981
- 1981-08-25 JP JP13384981A patent/JPS5825437B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5836383A (en) | 1983-03-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Dahl | Physicochemical aspects of disinfection of water by means of ultrasound and ozone | |
| Laroussi | Sterilization of contaminated matter with an atmospheric pressure plasma | |
| Epelle et al. | Ozone decontamination of medical and nonmedical devices: an assessment of design and implementation considerations | |
| US6984361B2 (en) | Use of continuous flow of Ox to control biological pathogens in mail and shipping parcels | |
| FR2542619A1 (en) | METHOD, COMPOSITION AND APPARATUS FOR STERILIZING USING GASEOUS CHLORINE BIOXIDE | |
| WO2019114749A1 (en) | Method for removing antibiotic resistance gene by using ionizing radiation | |
| CN1416465A (en) | Protecting molecules in biologically derived compsns.while treating with broad-spectrum pulsed light | |
| DE59008034D1 (en) | METHOD FOR STERILIZING MEDICAL WASTE. | |
| JP2901758B2 (en) | Infectious waste treatment | |
| SE507526C2 (en) | Sterilization method for closed packages | |
| Kuliesiene et al. | TiO2 Application for the Photocatalytical Inactivation of S. enterica, E. coli and M. luteus Bacteria Mixtures | |
| Fiester et al. | Electron beam irradiation dose dependently damages the Bacillus spore coat and spore membrane | |
| Thompson et al. | Gamma irradiation for inactivation of C. parvum, E. coli, and Coliphage MS-2 | |
| US20040052680A1 (en) | Treatment of environmental unit atmosphere and/or interior with generation of radicals | |
| JPS5825437B2 (en) | Biohazard protection method using ozone | |
| JPS6125688A (en) | Sterilizing method in germfree water manufacturing apparatus | |
| Ramsay et al. | The synergistic effect of excimer and low-pressure mercury lamps on the disinfection of flowing water | |
| CN113197199A (en) | Instrument disinfectant, and preparation method and application thereof | |
| JPH11505166A (en) | Dry sterilization method and apparatus for medical equipment | |
| JP2005534490A (en) | Apparatus and method for treating chemical and biological hazards | |
| JP5758006B2 (en) | Treatment of residual nucleic acids present on the surface of expendables for testing and testing | |
| JPS627833B2 (en) | ||
| US20070110611A1 (en) | Method of Disinfecting Items In a Vacuum Using Ozone | |
| JPH066144B2 (en) | Apparatus for sterilizing infectious waste by ozone and sterilization method using this apparatus | |
| Asadollahfardi et al. | Deactivation of Escherichia coli with different volumes in drinking water using cold atmospheric plasma |