JPS5844208B2 - Test piece - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a test piece for test that can easily detect chemical substances contained in various solutions by color reaction.

Currently, the test strip method is popular as a simple analysis method for measuring components contained in the body fluids of workers (eg, urine, blood), industrial wastewater, and river water.

The test strip used in this test strip method is one in which several types of reagents are impregnated into a single absorbent carrier, which is then cut into small pieces and pasted on the tip of a belt-shaped synthetic resin film, or simply. It is commercially available cut into strips.

However, in the case of conventional test pieces as mentioned above, since several types of reagents are contained in a single absorbent carrier, chemical reactions occur between the reagents during long-term storage, leading to decomposition and deterioration. However, in many cases, it cannot function as a test piece.

For example, test strips for measuring and detecting ketone bodies in human urine contain nitroprusid soda and an alkaline buffer, and since nitroprusid soda self-decomposes in alkaline conditions, it has traditionally been used as a film-forming substance. The two are separated.

However, because there is a film-forming substance between the two, the reaction rate when using the test piece becomes slow.
This method has the disadvantage of significantly lowering detection sensitivity.

In addition, attempts have been made to use test paper to measure phosphate ions in factory wastewater and industrial water, but this is similar to ketone body test paper because the main component, ammonium molybdate, reacts with strong acidic buffers such as trichloroacetic acid. However, the reality is that there are no good film-forming substances and no test paper has been successfully produced yet.

On the other hand, catecholamines increase in the body of patients with neuroblastoma, a neonatal cancer that has recently attracted particular attention, and VMA (Vanil 1y
el Mandelio Aoid) was found to be excreted in the urine, and diagnosis and early detection of neuroblastoma are performed by measuring this VMA.

In the VMA measurement method, the pH of a test solution (human urine) is usually prepared with an alkaline buffer, a diazotized paranitroaniline solution is added, a color reaction is caused, and the pH is measured with the naked eye or with a spectrophotometer.

(vMA detection method Sato: Modern Medicine 16:371.1961
), diazotized paranitroaniline, is very unstable and decomposed in alkaline conditions, similar to the above-mentioned ketone body detection reagent nitropurgide soda, so it has not been successfully made into a test strip.

Therefore, even now, the above-mentioned VMA testing method is used, and the preparation of reagents must be carried out for each test, which is a hassle.

Neuroblastoma has recently been on the rise, and early diagnosis must be carried out at children's hospitals and clinics. For this purpose, a simple test strip is needed, and the development of such a test strip is strongly desired.

In view of the current situation, the present inventor has completed the present invention as a result of various studies, and the gist of the present invention is as follows:
A band-shaped absorption carrier made by separately impregnating each reagent component constituting a color reaction system corresponding to the test solution is sequentially adjoined from the bottom to the top on the surface of a strip-shaped or cylindrical non-water-absorbing carrier. The test piece is characterized in that it is applied in such a way that the test solution can be chromatographically increased, and coloration occurs on the uppermost absorbing carrier.

In the present invention, the color reaction system refers to a system of components of reagents that are mixed so as to exhibit a color reaction to a detection component contained in a test solution.

Since the test strip of the present invention has the above-mentioned configuration, it is possible to completely avoid reactions between the various reagents, and therefore it has the advantage of being long-storable and convenient to carry.

The method of using the test piece of the present invention is to immerse part or all of the absorbent carrier attached to the leading edge in a test liquid.

In this way, the test solution moves up the chromatography, sequentially dissolving the reagents contained in the absorption carrier, and reaches the uppermost absorption carrier, where a color reaction occurs.

The target component in the test solution is simply measured by comparing the color change with a standard color change table prepared in advance.

As the absorbent carrier of the present invention, filter paper, nonwoven fabric, silica gel, alumina, etc. are used, and as the non-water absorbing carrier,
Various synthetic resin films, wood chips, thin steel plates, etc. can be used.

The adhesive may be any adhesive that does not react with the reagent contained in the absorption carrier.

A specific example of the shape of the test piece of the present invention is shown in FIG.

Figures A, B, and 0 are strip-shaped test pieces, the first drawing.

Although E and F indicate cylindrical test pieces, the shape of the test piece of the present invention is not limited to this, and other shapes and structures can be used as long as they do not depart from the gist of the present invention. .

In the figure, reference numerals 1, 2, 3, and 4 are absorption carriers pasted on a non-water-absorbing carrier, and these absorption carriers are each impregnated with a single reagent constituting the color reaction system.

A specific example of the test piece of the present invention will be given below.

For example, when detecting VMA in a test solution (human urine) for the diagnosis or early detection of neuroblastoma, a test piece is used, as shown in Figure 1A, with an alkaline buffer (2 g of sodium carbonate , consisting of borax, 11 water, 100 (1) pH 10,
Absorbent carrier 1 made by impregnating 1) with non-water absorbing carrier 5
The absorption carrier 2 made by impregnating diazotized varanitroaniline (0.1% aqueous solution) is attached to the upper part adjacent to the absorption carrier 1.

The method of using this test piece will be explained with reference to FIG.

As shown in Fig. 2A, place the test piece in the test solution 6 (VMA-containing human urine) in the container 7 with the absorbent carrier 1 facing down, and place the test piece up to about half of the tip absorbent carrier 1 as shown in Fig. 2B. Soak.

(For about 2 seconds) When the test solution 6 chromatographically rises up the absorption carrier 1, the alkaline buffer impregnated into the carrier is dissolved.
In this way, the pH of the rising test solution 6 is adjusted (PH=1o).

This elevated test solution 6 is further chromatographed onto the absorption carrier 2,
There, it reacts with the diazotized varanitroaniline impregnated into the carrier and exhibits coloration.

Next, as shown in FIG. 2C, the test piece was taken out from the test solution 6,
Immediately, the VMA concentration in the test solution 6 is measured by comparing it with a standard color change table prepared in advance.

Although the present invention has been specifically explained with regard to MA concentration in a test solution, for example, when measuring ketone bodies in human urine, phosphate ions in waste water, industrial water, etc., the coloration corresponds to the target test solution. Absorption carriers prepared by separately impregnating the individual reagents constituting the reaction system may be attached successively adjacent to each other on a non-water-absorbing carrier so that coloration occurs on the uppermost absorption carrier. The method for using it is the same as in the case of measuring the VMA concentration described above.

As described above, according to the present invention, with an extremely simple configuration,
It is possible to provide a test specimen that does not undergo any decomposition or deterioration due to chemical reactions between reagents even during long-term storage, and therefore can perform constant test and measurement functions with sufficient sensitivity. This produces a remarkable effect.

[Brief explanation of the drawing]

FIGS. 1 A-F are explanatory diagrams showing the specific structure of the test specimen of the present invention, and FIG. 2 A-0 is an explanatory diagram showing the specific structure of the test specimen of the present invention. ) is an explanatory diagram showing a mode of measuring. 1.2, 3, 4: Absorption carrier impregnated with reagent, 5: Non-water absorbing carrier, 6: Test liquid, 7: Container.

Claims (1)

[Claims] 1. A band-shaped absorption carrier prepared by separately impregnating each single reagent constituting the color reaction system corresponding to the test solution is sequentially applied from the bottom to the top on the surface of a strip-shaped or cylindrical non-water-absorbing carrier. A testing test piece O characterized in that the test solution is applied adjacently so that it can rise upwards, and coloration occurs on the uppermost absorbent carrier.