JPS588466B2 - electrophoresis analyzer - Google Patents
electrophoresis analyzerInfo
- Publication number
- JPS588466B2 JPS588466B2 JP52087160A JP8716077A JPS588466B2 JP S588466 B2 JPS588466 B2 JP S588466B2 JP 52087160 A JP52087160 A JP 52087160A JP 8716077 A JP8716077 A JP 8716077A JP S588466 B2 JPS588466 B2 JP S588466B2
- Authority
- JP
- Japan
- Prior art keywords
- analysis
- electrode tank
- injection port
- electrophoresis
- detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】 本発明は電気泳動分析装置に関する。[Detailed description of the invention] The present invention relates to an electrophoretic analysis device.
更に詳しくは、本発明は、電極槽と試料注入口及び検出
器を介設した泳動管と、前記電極槽に対して異極の電極
槽とを備えた電気泳動分析装置において、試料注入口と
検出器との間の泳動管に液体クロマトグラフ用充填剤を
充填し、電極槽と試料注入口との間及び検出器と異極の
電極槽との間に、それぞれ展開液流入・流出口を配設し
た電気泳動分析装置に関する。More specifically, the present invention provides an electrophoresis analyzer that includes an electrode tank, a sample injection port, and an electrophoresis tube in which a detector is interposed, and an electrode tank having a different polarity with respect to the electrode tank. Fill the electrophoresis tube between the detector with a packing material for liquid chromatography, and create developer inflow and outflow ports between the electrode tank and the sample injection port and between the detector and the electrode tank of different polarity. Regarding the installed electrophoresis analyzer.
細管式等速電気泳動分析方法は、泳動細管内部において
、ターミナル電解液とリーディング電解液の二種類の電
解液を充填し、この両電解液の境界面に荷電状態になる
物質、例えばアミノ酸類、ペプチド類、生体物質等の試
料を注入して定電流による電気泳動を行い、易動度の差
によって被検出物を単一ゾーンに分離し、適宜検出器に
て定性及び／又は定量するものであり、上述のような試
料の微量分析にはなくてはならないものとなっている。In the capillary isotachophoresis analysis method, two types of electrolytes, a terminal electrolyte and a leading electrolyte, are filled inside the electrophoresis capillary, and substances that become charged at the interface between the two electrolytes, such as amino acids, A sample of peptides, biological substances, etc. is injected and subjected to constant current electrophoresis, and the target substance is separated into a single zone based on the difference in mobility, and qualitatively and/or quantitatively determined using a detector as appropriate. It is indispensable for trace analysis of samples as mentioned above.
しかしながら、先般より試料の対象が多様化し、分離で
きない試料又はイオン性・非イオン性物質の混合試料の
同時分析が要求されるに至り、更に分析精度の高い電気
泳動分析装置の開発が期待されていた。However, recently, sample targets have become more diverse, and simultaneous analysis of samples that cannot be separated or mixed samples of ionic and nonionic substances has become necessary, and the development of electrophoretic analyzers with even higher analysis accuracy is expected. Ta.
本発明はシれらの事情に鑑みなされたものであり、その
構成上の主要な特徴の一つは、電気泳動分析装置の試料
注入口と検出器との間の泳動管に液体クロマトグラフ用
充填剤を充填し、電気泳動分析と同時に液体クロマトグ
ラフ分析を行うようにしたことにある。The present invention was made in view of these circumstances, and one of its main structural features is that a liquid chromatograph is used in the electrophoresis tube between the sample inlet and the detector of an electrophoresis analyzer. The reason is that it is filled with a packing material so that liquid chromatography analysis can be performed simultaneously with electrophoretic analysis.
本発明はこの特徴によって、目的物質を易動度の差のみ
ならず、充填剤による吸着、分配、交換等の分離機能を
付加し、分析精度を上昇させることができる。Due to this feature of the present invention, it is possible to increase analysis accuracy by adding separation functions such as adsorption, distribution, and exchange using a packing material as well as differences in mobility of the target substance.
本発明に係る電気泳動分析装置は、上述のように液体ク
ロマトグラフ分析による分離機能を具備しているが、そ
の為には勿論、展開液の供給が必要であり、電極槽と試
料注入口との間に展開液流入口、異極の電極槽と検出器
との間に展開液流出口をそれぞれ付設している。The electrophoretic analyzer according to the present invention has a separation function using liquid chromatography analysis as described above, but for this purpose, of course, it is necessary to supply a developing solution, and the electrode tank and sample injection port must be supplied. A developing solution inlet is provided between the two, and a developing solution outlet is provided between the electrode tank of different polarity and the detector.
従ってこの展開液の流出口を利用して容易に分離された
目的成分の分取を行うことができる。Therefore, the separated target component can be easily fractionated using the developing solution outlet.
本発明において、使用可能な充填剤としては、通常液体
クロマトグラフで使用されるものが挙げられる。In the present invention, examples of fillers that can be used include those commonly used in liquid chromatographs.
具体的には、デュポン社製のザイパックス、パーマフエ
ーズ、ゾルバックス等の充填剤が挙げられる。Specific examples include fillers such as Zypax, Permaphase, and Zorbax manufactured by DuPont.
本発明において両電極槽の極性は分析途中において逆に
してもよい。In the present invention, the polarities of both electrode vessels may be reversed during analysis.
例えば、展開液が流れる方向と試料イオンが泳動する方
向とが一致している場合は、十分な電気泳動分析を行う
為には長い泳動管が必要となるが、電極槽の極性を切換
えることによって短い泳動管でも十分分析が可能となる
。For example, if the direction in which the developing solution flows and the direction in which sample ions migrate are the same, a long migration tube is required to perform sufficient electrophoresis analysis, but by switching the polarity of the electrode tank, Analysis can be carried out even with a short electrophoresis tube.
勿論、交互に電極を切換えれば、試料に振動を与え分離
分散効果を更に高めることができる。Of course, by alternately switching the electrodes, it is possible to apply vibration to the sample and further enhance the separation and dispersion effect.
本発明においては、電解液は一種類のみ使用される。In the present invention, only one type of electrolyte is used.
このことが上述の電極の切り換えを可能にするものであ
る。This allows the switching of the electrodes described above.
以下、図に示す実施例に基いて本発明を詳述する。Hereinafter, the present invention will be explained in detail based on embodiments shown in the figures.
なお、これによって本発明が限定されるものではない。Note that the present invention is not limited to this.
図において、電気泳動分析装置１は、一電極槽２と、試
料注入口３及び紫外線吸収検出器４を介設したキャピラ
リチューブ５と、十電極槽６とを備えると共に、キャピ
ラリチューブの試料注入口３と紫外線吸収検出器４との
間には液体クロマトグラフ用充填剤の充填部７を形成し
ている。In the figure, an electrophoresis analyzer 1 includes a one-electrode tank 2, a capillary tube 5 in which a sample injection port 3 and an ultraviolet absorption detector 4 are interposed, a ten-electrode tank 6, and a sample injection port of the capillary tube. 3 and the ultraviolet absorption detector 4 is formed with a filling part 7 containing a liquid chromatography filler.
更にこの電気泳動分析装置１は、一電極槽２点試料注入
口３との間に展開液流入口８を、＋電極槽６と紫外線吸
収検出器４との間に流出口９を′それぞれ付設している
。Furthermore, this electrophoresis analyzer 1 is provided with a developer inlet 8 between the single electrode tank 2 points and the sample injection port 3, and an outlet 9 between the positive electrode tank 6 and the ultraviolet absorption detector 4. are doing.
なお１０は両電極槽２，６に定電流を供給する為の定電
流高圧電源である。Note that 10 is a constant current high voltage power supply for supplying constant current to both electrode tanks 2 and 6.
以上のような構成からなる電気泳動分析装置１の動作を
イオンの極性が−である泳動成分（例えばフタル酸類）
の分析を例に挙げて説明する。The operation of the electrophoresis analyzer 1 having the above-mentioned configuration is performed using electrophoretic components whose ion polarity is - (for example, phthalates).
This will be explained using an example of analysis.
まず、展開液流入口８より展開液（電解液）を定常状態
に流入し流出口９より流出するよう設定する。First, the developing solution (electrolyte) is set to flow into the developing solution inlet 8 in a steady state and to flow out from the outlet 9.
そして試料を試料注入口３より注入すると同時に定電流
高圧電源１０を作動させて電気泳動分析と液体クロマト
グラフ分析を併せて行なう。Then, at the same time as the sample is injected through the sample injection port 3, the constant current high voltage power supply 10 is activated to perform both electrophoretic analysis and liquid chromatography analysis.
即ち、電気泳動分析による易動度の差による分離に加え
て充填剤による吸着、分配、交換等による分離を行ない
、高精度の分離分析が可能となる。That is, in addition to separation based on the difference in mobility by electrophoretic analysis, separation is performed by adsorption, distribution, exchange, etc. using a packing material, making it possible to perform highly accurate separation analysis.
更に、具体的に説明を加えれば、目的成分がイオン性・
非イオン性成分よりなるとき、キャピラリチューブ５の
充填剤部７では、イオン性と非イオン性成分が分離され
るが、イオン性成分相互間の分離が十分でない場合、電
気泳動分析過程を経ることによって易動度の差を与える
ことができるのでそれだけ分離が細かくなり高精度の分
析が可能になる。Furthermore, to be more specific, if the target component is ionic or
When composed of nonionic components, the ionic and nonionic components are separated in the filler section 7 of the capillary tube 5, but if the separation between the ionic components is not sufficient, an electrophoretic analysis process is performed. Since it is possible to give a difference in mobility, the separation becomes finer and more precise analysis becomes possible.
ところで、このような分析の途中において、定電流高圧
電源１０の極性を逆に切り換えた場合は、試料イオンの
泳動方向が逆になり、滞留時間が長くなって確実な分離
が可能になる。By the way, if the polarity of the constant current high voltage power supply 10 is reversed during such an analysis, the migration direction of the sample ions will be reversed, the residence time will be extended, and reliable separation will be possible.
また、電気泳動のみでは非イオン性物質の分析は行えな
いが、液体クロマトグラフにより可能となる。Furthermore, although it is not possible to analyze nonionic substances using electrophoresis alone, it is possible using liquid chromatography.
なお、流出口９は目的物質の分取に用いることもできる
。Note that the outlet 9 can also be used for fractionating the target substance.
図面は本発明に係る電気泳動分析装置の一実施例を示す
機能説明図である。
１・・・・・・電気泳動分析装置、２，６・・・・・・
電極槽、３・・・・・・試料注入口、４・・・・・・検
出器、５・・・・・・キャピラリチューブ、７・・・・
・・充填部、８・・・・・・展開液流入口、９・・・・
・・展開液流出口。The drawing is a functional explanatory diagram showing one embodiment of the electrophoresis analyzer according to the present invention. 1... Electrophoresis analyzer, 2, 6...
Electrode tank, 3...Sample injection port, 4...Detector, 5...Capillary tube, 7...
...Filling section, 8...Development liquid inlet, 9...
・Developing solution outlet.
Claims (1)
、前記電極槽に対して異極の電極槽とを備えた電気泳動
分析装置において、試料注入口と検出器との間の泳動管
に液体クロマトグラフ用充填剤を充填し、電極槽と試料
注入口との間及び検出器と異極の電極槽との間に、それ
ぞれ展開液流入・流出口を配設し、展関液をその流入口
より定常状態に流入し流出口より流出するようにすると
共に、両電極槽に定電流電源を作動させることによって
、試料注入口より注入する試料を電気泳動分析と液体ク
ロマトグラフ分析の両分析に付せるよう構成してなる電
気泳動分析装置。1. In an electrophoresis analyzer equipped with a migration tube in which an electrode tank, a sample injection port, and a detector are interposed, and an electrode tank with a different polarity from the electrode tank, the electrophoresis between the sample injection port and the detector is The tube is filled with liquid chromatography packing material, and developing solution inflow and outflow ports are provided between the electrode tank and the sample injection port and between the detector and the electrode tank of different polarity. By making the sample flow into the inlet in a steady state and flowing out from the outlet, and by activating constant current power supplies to both electrode tanks, the sample injected from the sample injection port can be used for electrophoretic analysis and liquid chromatography analysis. An electrophoresis analyzer configured to be used for both types of analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP52087160A JPS588466B2 (en) | 1977-07-19 | 1977-07-19 | electrophoresis analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP52087160A JPS588466B2 (en) | 1977-07-19 | 1977-07-19 | electrophoresis analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5421795A JPS5421795A (en) | 1979-02-19 |
| JPS588466B2 true JPS588466B2 (en) | 1983-02-16 |
Family
ID=13907225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52087160A Expired JPS588466B2 (en) | 1977-07-19 | 1977-07-19 | electrophoresis analyzer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS588466B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60142252A (en) * | 1983-12-29 | 1985-07-27 | Shimadzu Corp | Liquid chromatography |
| JP2643266B2 (en) * | 1988-03-29 | 1997-08-20 | 東ソー株式会社 | Electrophoretic separation device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49127689A (en) * | 1973-04-07 | 1974-12-06 |
-
1977
- 1977-07-19 JP JP52087160A patent/JPS588466B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5421795A (en) | 1979-02-19 |
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